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Using different zebrafish models to explore liver regeneration. 利用不同的斑马鱼模型探索肝脏再生。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1485773
Dashuang Mo, Mengzhu Lv, Xiaoyu Mao

The liver possesses an impressive capability to regenerate following various injuries. Given its profound implications for the treatment of liver diseases, which afflict millions globally, liver regeneration stands as a pivotal area of digestive organ research. Zebrafish (Danio rerio) has emerged as an ideal model organism in regenerative medicine, attributed to their remarkable ability to regenerate tissues and organs, including the liver. Many fantastic studies have been performed to explore the process of liver regeneration using zebrafish, especially the extreme hepatocyte injury model. Biliary-mediated liver regeneration was first discovered in the zebrafish model and then validated in mammalian models and human patients. Considering the notable expansion of biliary epithelial cells in many end-stage liver diseases, the promotion of biliary-mediated liver regeneration might be another way to treat these refractory liver diseases. To date, a comprehensive review discussing the current advancements in zebrafish liver regeneration models is lacking. Therefore, this review aims to investigate the utility of different zebrafish models in exploring liver regeneration, highlighting the genetic and cellular insights gained and discussing the potential translational impact on human health.

肝脏在受到各种损伤后具有惊人的再生能力。肝脏再生对治疗困扰全球数百万人的肝脏疾病有着深远的影响,因此肝脏再生是消化器官研究的一个关键领域。斑马鱼(Danio rerio)因其卓越的组织和器官(包括肝脏)再生能力,已成为再生医学的理想模式生物。利用斑马鱼探索肝脏再生的过程,特别是极端肝细胞损伤模型,已经进行了许多精彩的研究。胆道介导的肝脏再生首先在斑马鱼模型中被发现,随后在哺乳动物模型和人类患者中得到验证。考虑到许多终末期肝病中胆道上皮细胞的显著扩张,促进胆道介导的肝脏再生可能是治疗这些难治性肝病的另一种方法。迄今为止,还缺乏对斑马鱼肝脏再生模型研究进展的全面综述。因此,本综述旨在研究不同斑马鱼模型在探索肝脏再生方面的效用,突出所获得的遗传和细胞见解,并讨论其对人类健康的潜在转化影响。
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引用次数: 0
Regulated cell death in chronic kidney disease: current evidence and future clinical perspectives. 慢性肾脏病中的调节性细胞死亡:现有证据和未来临床前景。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1497460
Kurt T K Giuliani, Benjamin C Adams, Helen G Healy, Andrew J Kassianos

Chronic kidney disease (CKD) is the progressive loss of kidney function/structure over a period of at least 3 months. It is characterised histologically by the triad of cell loss, inflammation and fibrosis. This literature review focuses on the forms of cell death that trigger downstream inflammation and fibrosis, collectively called regulated cell death (RCD) pathways. Discrete forms of RCD have emerged as central mediators of CKD pathology. In particular, pathways of regulated necrosis - including mitochondrial permeability transition pore (mPTP)-mediated necrosis, necroptosis, ferroptosis and pyroptosis - have been shown to mediate kidney pathology directly or through the release of danger signals that trigger a pro-inflammatory response, further amplifying tissue injury in a cellular process called necroinflammation. Despite accumulating evidence in pre-clinical models, no clinical studies have yet targeted these RCD modes in human CKD. The review summarizes recent advances in our understanding of RCD pathways in CKD, looks at inter-relations between the pathways (with the emphasis on propagation of death signals) and the evidence for therapeutic targeting of molecules in the RCD pathways to prevent or treat CKD.

慢性肾脏病(CKD)是指肾脏功能/结构在至少 3 个月内逐渐丧失。它的组织学特征是细胞丢失、炎症和纤维化三位一体。这篇文献综述的重点是引发下游炎症和纤维化的细胞死亡形式,统称为调控细胞死亡(RCD)途径。不同形式的 RCD 已成为慢性肾脏病病理学的核心介质。特别是,调节性坏死途径--包括线粒体通透性转换孔(mPTP)介导的坏死、坏死凋亡、铁凋亡和热凋亡--已被证明可直接或通过释放触发促炎症反应的危险信号介导肾脏病理,在称为坏死性炎症的细胞过程中进一步扩大组织损伤。尽管在临床前模型中积累了越来越多的证据,但目前还没有针对人类慢性肾脏病的这些 RCD 模式的临床研究。这篇综述总结了我们对 CKD 中 RCD 通路理解的最新进展,探讨了这些通路之间的相互关系(重点是死亡信号的传播)以及针对 RCD 通路中的分子进行治疗以预防或治疗 CKD 的证据。
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引用次数: 0
Chemical approaches targeting the hurdles of hepatocyte transplantation: mechanisms, applications, and advances. 针对肝细胞移植障碍的化学方法:机制、应用和进展。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1480226
Huanxiao Shi, Yi Ding, Pingxin Sun, Zhuman Lv, Chunyan Wang, Haoxin Ma, Junyu Lu, Bing Yu, Wenlin Li, Chao Wang

Hepatocyte transplantation (HTx) has been a novel cell-based therapy for severe liver diseases, as the donor livers for orthotopic liver transplantation are of great shortage. However, HTx has been confronted with two main hurdles: limited high-quality hepatocyte sources and low cell engraftment and repopulation rate. To cope with, researchers have investigated on various strategies, including small molecule drugs with unique advantages. Small molecules are promising chemical tools to modulate cell fate and function for generating high quality hepatocyte sources. In addition, endothelial barrier, immune responses, and low proliferative efficiency of donor hepatocytes mainly contributes to low cell engraftment and repopulation rate. Interfering these biological processes with small molecules is beneficial for improving cell engraftment and repopulation. In this review, we will discuss the applications and advances of small molecules in modulating cell differentiation and reprogramming for hepatocyte resources and in improving cell engraftment and repopulation as well as its underlying mechanisms.

肝细胞移植(HTx)是治疗严重肝病的一种新型细胞疗法,因为用于正位肝移植的供肝非常缺乏。然而,肝细胞移植一直面临着两大障碍:优质肝细胞来源有限以及细胞移植和再填充率低。为此,研究人员研究了各种策略,包括具有独特优势的小分子药物。小分子药物是一种很有前景的化学工具,可用于调节细胞的命运和功能,从而生成高质量的肝细胞来源。此外,供体肝细胞的内皮屏障、免疫反应和低增殖效率也是造成细胞移植和再填充率低的主要原因。用小分子干扰这些生物过程有利于提高细胞移植和再植率。在这篇综述中,我们将讨论小分子在调节肝细胞资源的细胞分化和重编程以及改善细胞移植和再植方面的应用和进展及其内在机制。
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引用次数: 0
Implications of DNA damage in chronic lung disease. DNA 损伤对慢性肺病的影响。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1436767
Dingning Zhang, Tong Sun, Jiahui Bao, Jianhua Fu

DNA plays an indispensable role in ensuring the perpetuation of life and safeguarding the genetic stability of living organisms. The emergence of diseases linked to a wide spectrum of responses to DNA damage has garnered increasing attention within the scientific community. There is growing evidence that patterns of DNA damage response in the lungs are associated with the onset, progression, and treatment of chronic lung diseases such as chronic obstructive pulmonary disease (COPD), asthma, and bronchopulmonary dysplasia (BPD). Currently, some studies have analyzed the mechanisms by which environmental factors induce lung DNA damage. In this article, we summarize inducible factors of lung DNA damage, current indicators, and methods for diagnosing DNA damage in chronic lung diseases and explore repair mechanisms after DNA damage including nonhomologous end-joining and homology-directed repair end joining pathways. Additionally, drug treatments that may reduce DNA damage or promote repair after it occurs in the lungs are briefly described. In general, more accurate assessment of the degree of lung DNA damage caused by various factors is needed to further elucidate the mechanism of lung DNA damage and repair after damage, so as to search for potential therapeutic targets.

DNA 在确保生命延续和保障生物体遗传稳定性方面发挥着不可或缺的作用。与 DNA 损伤反应有关的各种疾病的出现日益引起科学界的关注。越来越多的证据表明,肺部的 DNA 损伤反应模式与慢性阻塞性肺病(COPD)、哮喘和支气管肺发育不良(BPD)等慢性肺部疾病的发生、发展和治疗有关。目前,一些研究分析了环境因素诱导肺 DNA 损伤的机制。在本文中,我们总结了肺DNA损伤的诱导因素、目前的指标以及诊断慢性肺部疾病DNA损伤的方法,并探讨了DNA损伤后的修复机制,包括非同源末端连接和同源定向修复末端连接途径。此外,还简要介绍了可减少 DNA 损伤或促进肺部损伤后修复的药物治疗方法。总之,需要更准确地评估各种因素导致的肺DNA损伤程度,以进一步阐明肺DNA损伤和损伤后修复的机制,从而寻找潜在的治疗靶点。
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引用次数: 0
Enhancing human capillary tube network assembly and maturation through upregulated expression of pericyte-derived TIMP-3. 通过上调源于周细胞的 TIMP-3 的表达,增强人体毛细管网络的组装和成熟。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1465806
Ksenia Yrigoin, Kaitlyn N Bernard, Maria A Castaño, Ondine Cleaver, Saulius Sumanas, George E Davis

In this study, we identify and characterize new molecular determinants that optimize human capillary tube network assembly. Our lab has previously reported a novel, serum free-defined 3D co-culture model using human endothelial cells (ECs) and human pericytes whereby EC-lined tubes form and co-assemble with pericytes, but when these cultures are maintained at or beyond 5 days, tubes become progressively wider and unstable. To address this issue, we generated novel human pericytes that carry a tissue inhibitor of metalloproteinase (TIMP)-3 transgene which can be upregulated following doxycycline addition. EC-pericyte co-cultures established in the presence of doxycycline demonstrated marked enhancement of capillary network assembly including dramatic narrowing of capillary tube widths to an average of 8 µm (physiologic capillary tube width), increased tube lengths, increased tube branching, and robust stimulation of basement membrane matrix assembly, particularly with collagen type IV and fibronectin deposition compared to controls. These substantial changes depend not only on induction of pericyte TIMP-3, but also on recruitment of pericytes to EC tubes. Blockade of pericyte recruitment prevents these dramatic capillary network alterations suggesting that EC-pericyte interactions and induction of pericyte TIMP-3 are necessary together to coordinate and facilitate capillary assembly and maturation. Overall, this work is critical for our basic understanding of capillary formation, but also for the ability to reproducibly generate stabilized networks of capillary tubes.

在这项研究中,我们确定并描述了优化人类毛细管网络组装的新分子决定因素。我们的实验室以前曾报道过一种使用人内皮细胞(EC)和人周细胞的新型、无血清定义的三维共培养模型,在该模型中,EC内衬管与周细胞形成并共同组装,但当这些培养物维持5天或5天以上时,毛细管会逐渐变宽且不稳定。为了解决这个问题,我们生成了携带组织金属蛋白酶抑制剂(TIMP)-3 转基因的新型人周细胞,该转基因可在添加强力霉素后上调。与对照组相比,在多西环素存在下建立的EC-周细胞共培养物显示出毛细血管网络组装的明显增强,包括毛细血管管宽度急剧缩小至平均8微米(生理毛细血管管宽度)、管长度增加、管分支增加以及基底膜基质组装的强烈刺激,尤其是IV型胶原和纤维连接蛋白的沉积。这些实质性变化不仅取决于诱导周细胞 TIMP-3,还取决于将周细胞招募到心血管细胞管。阻断周细胞的募集可防止这些毛细血管网络的剧烈改变,这表明EC-周细胞的相互作用和周细胞TIMP-3的诱导对于协调和促进毛细血管的组装和成熟是必不可少的。总之,这项工作不仅对我们基本了解毛细血管的形成至关重要,而且对再现生成稳定的毛细血管网络的能力也至关重要。
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引用次数: 0
The role of STK11/LKB1 in cancer biology: implications for ovarian tumorigenesis and progression. STK11/LKB1 在癌症生物学中的作用:对卵巢肿瘤发生和发展的影响。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1449543
Jian Kang, Stefano Gallucci, Junqi Pan, Jonathan S Oakhill, Elaine Sanij

STK11 (serine-threonine kinase 11), also known as LKB1 (liver kinase B1) is a highly conserved master kinase that regulates cellular metabolism and polarity through a complex signaling network involving AMPK and 12 other AMPK-related kinases. Germline mutations in LKB1 have been causatively linked to Peutz-Jeghers Syndrome (PJS), an autosomal dominant hereditary disease with high cancer susceptibility. The identification of inactivating somatic mutations in LKB1 in different types of cancer further supports its tumor suppressive role. Deleterious mutations in LKB1 are frequently observed in patients with epithelial ovarian cancer. However, its inconsistent effects on tumorigenesis and cancer progression suggest that its functional impact is genetic context-dependent, requiring cooperation with other oncogenic lesions. In this review, we summarize the pleiotropic functions of LKB1 and how its altered activity in cancer cells is linked to oncogenic proliferation and growth, metastasis, metabolic reprogramming, genomic instability, and immune modulation. We also review the current mechanistic understandings of this master kinase as well as therapeutic implications with particular focus on the effects of LKB1 deficiency in ovarian cancer pathogenesis. Lastly, we discuss whether LKB1 deficiency can be exploited as an Achilles heel in ovarian cancer.

STK11(丝氨酸-苏氨酸激酶 11)又称 LKB1(肝激酶 B1),是一种高度保守的主激酶,它通过涉及 AMPK 和其他 12 种 AMPK 相关激酶的复杂信号网络来调节细胞的新陈代谢和极性。LKB1 基因突变与 Peutz-Jeghers 综合征(PJS)有因果关系,PJS 是一种常染色体显性遗传病,具有高癌症易感性。在不同类型的癌症中发现了 LKB1 的失活体细胞突变,这进一步证实了其抑制肿瘤的作用。在上皮性卵巢癌患者中,经常可以观察到 LKB1 的畸变突变。然而,LKB1 对肿瘤发生和癌症进展的影响并不一致,这表明它的功能影响取决于基因环境,需要与其他致癌病变合作。在这篇综述中,我们总结了 LKB1 的多效应功能,以及它在癌细胞中的活性改变是如何与致癌增殖和生长、转移、代谢重编程、基因组不稳定性和免疫调节联系在一起的。我们还回顾了目前对这种主激酶的机理认识以及治疗意义,尤其关注 LKB1 缺乏对卵巢癌发病机制的影响。最后,我们将讨论 LKB1 缺乏是否可作为卵巢癌的致命弱点加以利用。
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引用次数: 0
Editorial: Unlocking the potential of cell therapy: exploring cell types, induction methods, and culture techniques. 社论:释放细胞疗法的潜力:探索细胞类型、诱导方法和培养技术。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1515978
Viknesvaran Selvarajan, Samuel Ken-En Gan, Yuen Ling Ng
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引用次数: 0
Predicting an opaque bubble layer during small-incision lenticule extraction surgery based on deep learning. 基于深度学习预测小切口光阑摘除手术中的不透明气泡层。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1487482
Zeyu Zhu, Xiang Zhang, Qing Wang, Jian Xiong, Jingjing Xu, Kang Yu, Zheliang Guo, Shaoyang Xu, Mingyan Wang, Yifeng Yu

Aim: This study aimed to predict the formation of OBL during femtosecond laser SMILE surgery by employing deep learning technology.

Methods: This was a cross-sectional, retrospective study conducted at a university hospital. Surgical videos were randomly divided into a training (3,271 patches, 73.64%), validation (704 patches, 15.85%), and internal verification set (467 patches, 10.51%). An artificial intelligence (AI) model was developed using a SENet-based residual regression deep neural network. Model performance was assessed using the mean absolute error (E MA ), Pearson's correlation coefficient (r), and determination coefficient (R 2 ).

Results: Four distinct types of deep neural network models were established. The modified deep residual neural network prediction model with channel attention built on the PyTorch framework demonstrated the best predictive performance. The predicted OBL area values correlated well with the Photoshop-based measurements (E MA = 0.253, r = 0.831, R 2 = 0.676). The ResNet (E MA = 0.259, r = 0.798, R 2 = 0.631) and Vgg19 models (E MA = 0.31, r = 0.758, R 2 = 0.559) both displayed satisfactory predictive performance, while the U-net model (E MA = 0.605, r = 0.331, R 2 = 0.171) performed poorest.

Conclusion: We used a panoramic corneal image obtained before the SMILE laser scan to create a unique deep residual neural network prediction model to predict OBL formation during SMILE surgery. This model demonstrated exceptional predictive power, suggesting its clinical applicability across a broad field.

目的:本研究旨在利用深度学习技术预测飞秒激光SMILE手术中OBL的形成:这是一项在大学医院进行的横断面回顾性研究。手术视频被随机分为训练集(3271 个补丁,占 73.64%)、验证集(704 个补丁,占 15.85%)和内部验证集(467 个补丁,占 10.51%)。使用基于 SENet 的残差回归深度神经网络开发了一个人工智能 (AI) 模型。使用平均绝对误差(E MA)、皮尔逊相关系数(r)和判定系数(R 2)评估模型性能:结果:建立了四种不同类型的深度神经网络模型。基于 PyTorch 框架建立的具有通道关注度的改进型深度残差神经网络预测模型显示出最佳预测性能。预测的 OBL 面积值与基于 Photoshop 的测量值相关性良好(E MA = 0.253,r = 0.831,R 2 = 0.676)。ResNet 模型(E MA = 0.259,r = 0.798,R 2 = 0.631)和 Vgg19 模型(E MA = 0.31,r = 0.758,R 2 = 0.559)的预测性能都令人满意,而 U-net 模型(E MA = 0.605,r = 0.331,R 2 = 0.171)的性能最差:我们利用 SMILE 激光扫描前获得的角膜全景图像创建了一个独特的深度残差神经网络预测模型,用于预测 SMILE 手术中 OBL 的形成。该模型显示出卓越的预测能力,表明其临床应用领域广泛。
{"title":"Predicting an opaque bubble layer during small-incision lenticule extraction surgery based on deep learning.","authors":"Zeyu Zhu, Xiang Zhang, Qing Wang, Jian Xiong, Jingjing Xu, Kang Yu, Zheliang Guo, Shaoyang Xu, Mingyan Wang, Yifeng Yu","doi":"10.3389/fcell.2024.1487482","DOIUrl":"10.3389/fcell.2024.1487482","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to predict the formation of OBL during femtosecond laser SMILE surgery by employing deep learning technology.</p><p><strong>Methods: </strong>This was a cross-sectional, retrospective study conducted at a university hospital. Surgical videos were randomly divided into a training (3,271 patches, 73.64%), validation (704 patches, 15.85%), and internal verification set (467 patches, 10.51%). An artificial intelligence (AI) model was developed using a SENet-based residual regression deep neural network. Model performance was assessed using the mean absolute error (<i>E</i> <sub><i>MA</i></sub> ), Pearson's correlation coefficient (<i>r</i>), and determination coefficient (<i>R</i> <sup><i>2</i></sup> ).</p><p><strong>Results: </strong>Four distinct types of deep neural network models were established. The modified deep residual neural network prediction model with channel attention built on the PyTorch framework demonstrated the best predictive performance. The predicted OBL area values correlated well with the Photoshop-based measurements (<i>E</i> <sub><i>MA</i></sub> = 0.253, <i>r</i> = 0.831, <i>R</i> <sup><i>2</i></sup> = 0.676). The ResNet (<i>E</i> <sub><i>MA</i></sub> = 0.259, <i>r</i> = 0.798, <i>R</i> <sup><i>2</i></sup> = 0.631) and Vgg19 models (<i>E</i> <sub><i>MA</i></sub> = 0.31, <i>r</i> = 0.758, <i>R</i> <sup><i>2</i></sup> = 0.559) both displayed satisfactory predictive performance, while the U-net model (<i>E</i> <sub><i>MA</i></sub> = 0.605, <i>r</i> = 0.331, <i>R</i> <sup><i>2</i></sup> = 0.171) performed poorest.</p><p><strong>Conclusion: </strong>We used a panoramic corneal image obtained before the SMILE laser scan to create a unique deep residual neural network prediction model to predict OBL formation during SMILE surgery. This model demonstrated exceptional predictive power, suggesting its clinical applicability across a broad field.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1487482"},"PeriodicalIF":4.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening of pathologically significant diagnostic biomarkers in tears of thyroid eye disease based on bioinformatic analysis and machine learning. 基于生物信息分析和机器学习筛选甲状腺眼病泪液中具有重要病理诊断意义的生物标记物。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1486170
Xingyi Shu, Chengcheng Zeng, Yanfei Zhu, Yuqing Chen, Xiao Huang, Ruili Wei

Background: Lacrimal gland enlargement is a common pathological change in patients with thyroid eye disease (TED). Tear fluid has emerged as a new source of diagnostic biomarkers, but tear-based diagnostic biomarkers for TED with high efficacy are still lacking.

Objective: We aim to investigate genes associated with TED-associated lacrimal gland lesions. Additionally, we seek to identify potential biomarkers for diagnosing TED in tear fluid.

Methods: We obtained two expression profiling datasets related to TED lacrimal gland samples from the Gene Expression Omnibus (GEO). Subsequently, we combined the two separate datasets and conducted differential gene expression analysis and weighted gene co-expression network analysis (WGCNA) on the obtained integrated dataset. The genes were employed for Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The genes were intersected with the secretory proteins profile to get the potential proteins in the tear fluid. Machine learning techniques were then employed to identify optimal biomarkers and develop a diagnostic nomogram for predicting TED. Finally, gene set enrichment analysis (GSEA) and immune infiltration analysis were conducted on screened hub genes to further elucidate their potential mechanisms in TED.

Results: In our analysis of the integrated TED dataset, we identified 2,918 key module genes and 157 differentially expressed genes and finally obtained 84 lacrimal-associated key genes. Enrichment analysis disclosed that these 84 genes primarily pertain to endoplasmic reticulum organization. After intersecting with the secretory proteins, 13 lacrimal gland-associated secretory protein genes (LaSGs) were identified. The results from machine learning indicated the substantial diagnostic value of dyslexia associated gene (KIAA0319) and peroxiredoxin4 (PRDX4) in TED-associated lacrimal gland lesions. The two hub genes were chosen as candidate biomarkers in tear fluid and employed to establish a diagnostic nomogram. Furthermore, single-gene GSEA results and immune cell infiltration analysis unveiled immune dysregulation in the lacrimal gland of TED, with KIAA0319 and PRDX4 showing significant associations with infiltrating immune cells.

Conclusions: We uncovered the distinct pathophysiology of TED-associated lacrimal gland enlargement compared to TED-associated orbital adipose tissue enlargement. We have demonstrated the endoplasmic reticulum-related pathways involved in TED-associated lacrimal gland lesions and established a diagnostic nomogram for TED utilizing KIAA0319 and PRDX4 through integrated bioinformatics analysis. This contribution offers novel insights for non-invasive, prospective diagnostic approaches in the context of TED.

背景:泪腺肿大是甲状腺眼病(TED)患者常见的病理变化。泪液已成为诊断生物标志物的新来源,但基于泪液的TED高效诊断生物标志物仍然缺乏:我们旨在研究与 TED 相关的泪腺病变的相关基因。此外,我们还试图确定诊断泪液中 TED 的潜在生物标志物:我们从基因表达总库(GEO)中获得了两个与 TED 泪腺样本相关的表达谱数据集。随后,我们合并了这两个独立的数据集,并对整合后的数据集进行了差异基因表达分析和加权基因共表达网络分析(WGCNA)。这些基因被用于基因本体(GO)富集分析和京都基因组百科全书(KEGG)通路分析。将这些基因与分泌蛋白图谱进行交叉,以获得泪液中的潜在蛋白。然后采用机器学习技术来确定最佳生物标志物,并开发出用于预测 TED 的诊断提名图。最后,对筛选出的枢纽基因进行了基因组富集分析(GSEA)和免疫浸润分析,以进一步阐明它们在TED中的潜在机制:结果:在对整合的 TED 数据集进行分析时,我们发现了 2,918 个关键模块基因和 157 个差异表达基因,并最终获得了 84 个泪腺相关关键基因。富集分析显示,这84个基因主要与内质网组织有关。在与分泌蛋白交叉后,确定了 13 个泪腺相关分泌蛋白基因(LaSGs)。机器学习的结果表明,阅读障碍相关基因(KIAA0319)和过氧化物歧化酶4(PRDX4)在TED相关泪腺病变中具有重要的诊断价值。这两个枢纽基因被选为泪液中的候选生物标志物,并被用于建立诊断提名图。此外,单基因GSEA结果和免疫细胞浸润分析揭示了TED泪腺中的免疫失调,其中KIAA0319和PRDX4与浸润免疫细胞有显著关联:我们发现了TED相关泪腺肿大与TED相关眼眶脂肪组织肿大不同的病理生理学。我们证明了参与 TED 相关泪腺病变的内质网相关通路,并通过综合生物信息学分析,利用 KIAA0319 和 PRDX4 建立了 TED 诊断提名图。这一贡献为 TED 的无创、前瞻性诊断方法提供了新的见解。
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引用次数: 0
Unveiling the dynamics of circulating tumor cells in colorectal cancer: from biology to clinical applications. 揭示结直肠癌循环肿瘤细胞的动态:从生物学到临床应用。
IF 4.6 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.3389/fcell.2024.1498032
Claudia Dompé, Aleksandra Chojnowska, Rodryg Ramlau, Michal Nowicki, Catherine Alix-Panabières, Joanna Budna-Tukan

This review delves into the pivotal role of circulating tumor cells (CTCs) in colorectal cancer (CRC) metastasis, focusing on their biological properties, interactions with the immune system, advanced detection techniques, and clinical implications. We explored how metastasis-competent CTCs evade immune surveillance and proliferate, utilizing cutting-edge detection and isolation technologies, such as microfluidic devices and immunological assays, to enhance sensitivity and specificity. The review highlights the significant impact of CTC interactions with immune cells on tumor progression and patient outcomes. It discusses the application of these findings in clinical settings, including non-invasive liquid biopsies for early diagnosis, prognosis, and treatment monitoring. Despite advancements, challenges remain, such as the need for standardized methods to consistently capture and analyze CTCs. Addressing these challenges through further molecular and cellular research on CTCs could lead to improved interventions and outcomes for CRC patients, underscoring the importance of unraveling the complex dynamics of CTCs in cancer progression.

这篇综述深入探讨了循环肿瘤细胞(CTC)在结直肠癌(CRC)转移中的关键作用,重点关注它们的生物特性、与免疫系统的相互作用、先进的检测技术以及临床意义。我们探讨了具有转移能力的 CTC 如何逃避免疫监视并增殖,利用微流体设备和免疫测定等尖端检测和分离技术提高灵敏度和特异性。综述强调了 CTC 与免疫细胞的相互作用对肿瘤进展和患者预后的重大影响。它讨论了这些发现在临床中的应用,包括用于早期诊断、预后判断和治疗监测的无创液体活检。尽管取得了进步,但挑战依然存在,例如需要标准化的方法来持续捕获和分析 CTCs。通过对 CTCs 的进一步分子和细胞研究来应对这些挑战,可以改善对 CRC 患者的干预和治疗效果,这凸显了揭示 CTCs 在癌症进展中的复杂动态的重要性。
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引用次数: 0
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Frontiers in Cell and Developmental Biology
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