Bamboo resources have garnered significant global attention due to their excellent capacity for regeneration and high yield. Rhombic-spot disease, a substantial threat to fishscale bamboo (Phyllostachys heteroclada), is primarily caused by Neostagonosporella sichuanensis. This study first reported the genome assemblies and characteristics of two N. sichuanensis isolates using PacBio and Illumina sequencing platforms. The genomes of N. sichuanensis strain SICAUCC 16–0001 and strain SICAUCC 23–0140, with sizes of 48.0 Mb and 48.4 Mb, respectively, revealed 10,289 and 10,313 protein-coding genes. Additionally, they contained 34.99 and 34.46% repetitive sequences within AT-rich regions, with notable repeat-induced point mutation activity. Comparative genome analysis identified 1,049 contracted and 45 expanded gene families in the genome of N. sichuanensis, including several related to pathogenicity. Several gene families involved in mycotoxin metabolism, secondary metabolism, sterol biosynthesis and transport, and cell wall degradation were contracted. Compared to most analyzed necrotrophic, hemibiotrophic, and phaeosphaeriacous pathogens, the genomes of two N. sichuanensis isolates exhibited fewer secondary metabolite enzymes, carbohydrate-active enzymes, plant cell wall degrading enzymes, secreted proteins, and effectors. Comparative genomics analysis suggested that N. sichuanensis shares more similar characteristics with hemibiotrophic pathogens. Based on single carbon source tests, N. sichuanensis strains demonstrated a higher potential for xylan decomposition than pectin and cellulose. The proportion of cell wall-degrading enzyme effectors occupied a high proportion of the total effectors of the N. sichuanensis genomes. These findings provide valuable insights into uncovering the pathogenesis of N. sichuanensis toward the efficient management of rhombic-spot disease of fishscale bamboo.
{"title":"Genome-wide survey of the bipartite structure and pathogenesis-related genes of Neostagonosporella sichuanensis, a causal agent of Fishscale bamboo rhombic-spot disease","authors":"Lijuan Liu, Chunlin Yang, Fang Liang, Chengsong Li, Qian Zeng, Shan Han, Shujiang Li, Yinggao Liu","doi":"10.3389/fmicb.2024.1456993","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1456993","url":null,"abstract":"Bamboo resources have garnered significant global attention due to their excellent capacity for regeneration and high yield. Rhombic-spot disease, a substantial threat to fishscale bamboo (<jats:italic>Phyllostachys heteroclada</jats:italic>), is primarily caused by <jats:italic>Neostagonosporella sichuanensis</jats:italic>. This study first reported the genome assemblies and characteristics of two <jats:italic>N. sichuanensis</jats:italic> isolates using PacBio and Illumina sequencing platforms. The genomes of <jats:italic>N. sichuanensis</jats:italic> strain SICAUCC 16–0001 and strain SICAUCC 23–0140, with sizes of 48.0 Mb and 48.4 Mb, respectively, revealed 10,289 and 10,313 protein-coding genes. Additionally, they contained 34.99 and 34.46% repetitive sequences within AT-rich regions, with notable repeat-induced point mutation activity. Comparative genome analysis identified 1,049 contracted and 45 expanded gene families in the genome of <jats:italic>N. sichuanensis</jats:italic>, including several related to pathogenicity. Several gene families involved in mycotoxin metabolism, secondary metabolism, sterol biosynthesis and transport, and cell wall degradation were contracted. Compared to most analyzed necrotrophic, hemibiotrophic, and phaeosphaeriacous pathogens, the genomes of two <jats:italic>N. sichuanensis</jats:italic> isolates exhibited fewer secondary metabolite enzymes, carbohydrate-active enzymes, plant cell wall degrading enzymes, secreted proteins, and effectors. Comparative genomics analysis suggested that <jats:italic>N. sichuanensis</jats:italic> shares more similar characteristics with hemibiotrophic pathogens. Based on single carbon source tests, <jats:italic>N. sichuanensis</jats:italic> strains demonstrated a higher potential for xylan decomposition than pectin and cellulose. The proportion of cell wall-degrading enzyme effectors occupied a high proportion of the total effectors of the <jats:italic>N. sichuanensis</jats:italic> genomes. These findings provide valuable insights into uncovering the pathogenesis of <jats:italic>N. sichuanensis</jats:italic> toward the efficient management of rhombic-spot disease of fishscale bamboo.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1447735
Zhiyu Chen, Shuo Zhang, Xiaodong Sun, Duo Meng, Chencen Lai, Min Zhang, Pengjiao Wang, Xuncai Huang, Xiuli Gao
Acute lung injury (ALI) is a severe pulmonary condition with high mortality and morbidity, lacking effective pharmacotherapeutic options. Rosa roxburghii Tratt, a unique fruit from southwestern China, is valued for its rich nutritional content and functional properties. Fermentation is known to enhance the nutritional value, flavor, and shelf life of foods. In this study, we investigated the effects of fermented Rosa roxburghii juice (RRFJ) on gut microbiota, metabolites, and the levels of short-chain fatty acids in the intestines, as well as its impact on lung tissue and intestine tissue injury, inflammation, and oxidative stress in murine models. The results showed that RRFJ modulated gut microbiota and metabolites, increased short-chain fatty acid levels, and consequently reduced lung tissue injury, inflammation, and oxidative stress in mice with ALI. These findings suggest that RRFJ has the potential to serve as a functional dietary adjunct in the management of acute lung injury, providing a scientific basis for its therapeutic role.
急性肺损伤（ALI）是一种严重的肺部疾病，死亡率和发病率都很高，但缺乏有效的药物治疗方案。特拉特蔷薇（Rosa roxburghii Tratt）是一种产自中国西南部的独特水果，因其丰富的营养成分和功能特性而备受重视。众所周知，发酵可提高食品的营养价值、风味和保质期。在这项研究中，我们研究了发酵罗布麻汁（RRFJ）对肠道微生物群、代谢物和肠道短链脂肪酸水平的影响，以及对小鼠模型肺组织和肠道组织损伤、炎症和氧化应激的影响。结果表明，RRFJ 可调节肠道微生物群和代谢物，提高短链脂肪酸水平，从而减轻 ALI 小鼠的肺组织损伤、炎症和氧化应激。这些研究结果表明，RRFJ 有可能成为治疗急性肺损伤的功能性膳食辅助剂，为其治疗作用提供了科学依据。
{"title":"Rosa roxburghii fermented juice mitigates LPS-induced acute lung injury by modulation of intestinal flora and metabolites","authors":"Zhiyu Chen, Shuo Zhang, Xiaodong Sun, Duo Meng, Chencen Lai, Min Zhang, Pengjiao Wang, Xuncai Huang, Xiuli Gao","doi":"10.3389/fmicb.2024.1447735","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1447735","url":null,"abstract":"Acute lung injury (ALI) is a severe pulmonary condition with high mortality and morbidity, lacking effective pharmacotherapeutic options. <jats:italic>Rosa roxburghii</jats:italic> Tratt, a unique fruit from southwestern China, is valued for its rich nutritional content and functional properties. Fermentation is known to enhance the nutritional value, flavor, and shelf life of foods. In this study, we investigated the effects of fermented <jats:italic>Rosa roxburghii</jats:italic> juice (RRFJ) on gut microbiota, metabolites, and the levels of short-chain fatty acids in the intestines, as well as its impact on lung tissue and intestine tissue injury, inflammation, and oxidative stress in murine models. The results showed that RRFJ modulated gut microbiota and metabolites, increased short-chain fatty acid levels, and consequently reduced lung tissue injury, inflammation, and oxidative stress in mice with ALI. These findings suggest that RRFJ has the potential to serve as a functional dietary adjunct in the management of acute lung injury, providing a scientific basis for its therapeutic role.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1462749
Jiatong Lu, Qiang Tong
The intricate relationship between cancer and bacteria has garnered increasing attention in recent years. While traditional cancer research has primarily focused on tumor cells and genetic mutations, emerging evidence highlights the significant role of microbial communities within the tumor microenvironment in cancer development and progression. This review aims to provide a comprehensive overview of the current understanding of the complex interplay between cancer and bacteria. We explore the diverse ways in which bacteria influence tumorigenesis and tumor behavior, discussing direct interactions between bacteria and tumor cells, their impact on tumor immunity, and the potential modulation of the tumor microenvironment. Additionally, we delve into the mechanisms through which bacterial metabolites and extracellular products May affect cancer pathways. By conducting a thorough analysis of the existing literature, we underscore the multifaceted and intricate relationship between bacteria and cancer. Understanding this complex interplay could pave the way for novel therapeutic approaches and preventive strategies in cancer treatment.
{"title":"From pathogenesis to treatment: the impact of bacteria on cancer","authors":"Jiatong Lu, Qiang Tong","doi":"10.3389/fmicb.2024.1462749","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1462749","url":null,"abstract":"The intricate relationship between cancer and bacteria has garnered increasing attention in recent years. While traditional cancer research has primarily focused on tumor cells and genetic mutations, emerging evidence highlights the significant role of microbial communities within the tumor microenvironment in cancer development and progression. This review aims to provide a comprehensive overview of the current understanding of the complex interplay between cancer and bacteria. We explore the diverse ways in which bacteria influence tumorigenesis and tumor behavior, discussing direct interactions between bacteria and tumor cells, their impact on tumor immunity, and the potential modulation of the tumor microenvironment. Additionally, we delve into the mechanisms through which bacterial metabolites and extracellular products May affect cancer pathways. By conducting a thorough analysis of the existing literature, we underscore the multifaceted and intricate relationship between bacteria and cancer. Understanding this complex interplay could pave the way for novel therapeutic approaches and preventive strategies in cancer treatment.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Droplet microfluidic-based technology is a powerful tool for biotechnology, and it is also expected that it will be applied to culturing and screening methods. Using this technology, a new high-throughput screening method for lactic acid bacteria was developed. In this study, the conventional culture of lactic acid bacteria that form clear zones on an agar medium was reproduced in water-in-oil droplets, and only the droplets in which lactic acid bacteria grew were collected one by one. Using this method, the specific recovery of Lactiplantibacillus plantarum from a mixture of L. plantarum and Escherichia coli and the acquirement of lactic acid bacteria from an environmental sample were successful. This method could be applied to various conventional screening methods using the clear zone as a microbial growth indicator. This has expanded the possibilities of applying droplet microfluidic-based technology to microbial cultivations.
{"title":"Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria","authors":"Koji Mori, Mizuki Watanabe, Keiko Nanri, Satoko Matsukura, Yuri Ota, Nobuyuki Homma, Naohiro Noda","doi":"10.3389/fmicb.2024.1452573","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1452573","url":null,"abstract":"Droplet microfluidic-based technology is a powerful tool for biotechnology, and it is also expected that it will be applied to culturing and screening methods. Using this technology, a new high-throughput screening method for lactic acid bacteria was developed. In this study, the conventional culture of lactic acid bacteria that form clear zones on an agar medium was reproduced in water-in-oil droplets, and only the droplets in which lactic acid bacteria grew were collected one by one. Using this method, the specific recovery of <jats:italic>Lactiplantibacillus plantarum</jats:italic> from a mixture of <jats:italic>L. plantarum</jats:italic> and <jats:italic>Escherichia coli</jats:italic> and the acquirement of lactic acid bacteria from an environmental sample were successful. This method could be applied to various conventional screening methods using the clear zone as a microbial growth indicator. This has expanded the possibilities of applying droplet microfluidic-based technology to microbial cultivations.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1437036
Mengdi Zhao, Yueyao Li, Yuanyuan Zhang, Guangyu Li
Probiotics are one of the management tools to improve the host’s healthy microbiota. The positive effects of probiotics on host health are species-specific, so probiotics isolated from host’s own gut may be most beneficial. Many of the metabolites (e.g., short-chain fatty acids, bacteriocins, and hydrogen peroxide) produced by Lactobacillus johnsonii have specific inhibitory profiles against invading pathogens. In this study, we isolated L. johnsonii GJ231 from the intestinal tract of healthy female beagles. The genome size of 1.763 M encoded a total of 1,691 predicted genes. Many carbohydrate-active enzymes responsible for carbohydrate degradation and the production of short-chain fatty acids were also predicted. The metabolic profile of short-chain fatty acids in L. johnsonii GJ231 was determined using LC–MS/MS. The bacteriocin-producing gene bacteriocin (lactacin F) in L. johnsonii GJ231 was also predicted. In vitro, experiments demonstrated that GJ231 can thrive in weak acids, 0.3% bile salts, and artificial gastrointestinal fluid models. It was tolerant of to high temperatures up to 70°C, was non- hemolytic, inhibited pathogenic bacteria, and had a high antioxidant capacity. In vivo safety experiments conducted in mice revealed that oral administration of GJ231 not only had no toxic side effect but also increased their antioxidant capacity. In conclusion, combining the above test results, which collectively demonstrate that canine-derived L. johnsonii GJ231 was a non-pathogenic, acid-tolerant and bile-salt-tolerant probiotic strain that inhibits pathogenic bacteria and improves host antioxidant function. This may make it a promising candidate for the development of innovative functional foods for pets.
{"title":"Genomic analysis and functional properties of Lactobacillus johnsonii GJ231 isolated from healthy beagles","authors":"Mengdi Zhao, Yueyao Li, Yuanyuan Zhang, Guangyu Li","doi":"10.3389/fmicb.2024.1437036","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1437036","url":null,"abstract":"Probiotics are one of the management tools to improve the host’s healthy microbiota. The positive effects of probiotics on host health are species-specific, so probiotics isolated from host’s own gut may be most beneficial. Many of the metabolites (e.g., short-chain fatty acids, bacteriocins, and hydrogen peroxide) produced by <jats:italic>Lactobacillus johnsonii</jats:italic> have specific inhibitory profiles against invading pathogens. In this study, we isolated <jats:italic>L. johnsonii</jats:italic> GJ231 from the intestinal tract of healthy female beagles. The genome size of 1.763 M encoded a total of 1,691 predicted genes. Many carbohydrate-active enzymes responsible for carbohydrate degradation and the production of short-chain fatty acids were also predicted. The metabolic profile of short-chain fatty acids in <jats:italic>L. johnsonii</jats:italic> GJ231 was determined using LC–MS/MS. The bacteriocin-producing gene bacteriocin (lactacin F) in <jats:italic>L. johnsonii</jats:italic> GJ231 was also predicted. <jats:italic>In vitro</jats:italic>, experiments demonstrated that GJ231 can thrive in weak acids, 0.3% bile salts, and artificial gastrointestinal fluid models. It was tolerant of to high temperatures up to 70°C, was non- hemolytic, inhibited pathogenic bacteria, and had a high antioxidant capacity. <jats:italic>In vivo</jats:italic> safety experiments conducted in mice revealed that oral administration of GJ231 not only had no toxic side effect but also increased their antioxidant capacity. In conclusion, combining the above test results, which collectively demonstrate that canine-derived <jats:italic>L. johnsonii</jats:italic> GJ231 was a non-pathogenic, acid-tolerant and bile-salt-tolerant probiotic strain that inhibits pathogenic bacteria and improves host antioxidant function. This may make it a promising candidate for the development of innovative functional foods for pets.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1417598
Jing Yang, Yun-Ze Chen, Guo-Cai Zhang
IntroductionThe gut bacteria of insects play an important role in regulating their metabolism, immune system and metabolizing pesticides. Our previous results indicate that carvacrol has certain gastric toxic activity on Lymantria dispar larvae and affects their detoxification metabolism at the mRNA level. However, the impact of carvacrol on the gut bacteria of L. dispar larvae has been unclear.MethodsIn this study, the 16S rRNA sequencing technology was used to sequence and analyze the gut bacteria of the larvae which were exposed with sublethal concentration (0.297 mg/mL) and median lethal concentration (1.120 mg/mL), respectively.ResultsA total of 10 phyla, 16 classes, 47 orders, 72 families, 103 genera, and 135 species were obtained by using a 97% similarity cutoff level. The dominant bacterial phyla in the gut of the L. dispar larvae are Firmicutes and Proteobacteria. The treatment with carvacrol can significantly affect the structure of gut bacteria in the larvae of the L. dispar. At both doses, carvacrol can shift the dominant gut bacteria of the larvae from Proteobacteria to Firmicutes. At the genus level, two doses of carvacrol can significantly enhance the relative abundance of probiotic Lactobacillus in the gut of L. dispar larvae (p ≤ 0.01). Additionally, significant differences were observed among the five bacterial genera Burkholderia-Caballeronia-Paraburkholderia, Anoxybacillus, Pelomonas, Mesorhizobium (p ≤ 0.05). The analysis of α-diversity and β-diversity indicates that the treatment with carvacrol at two doses significantly affect the bacterial richness and diversity in the larvae. However, the results of functional classification prediction (PICRUSt) indicate that carvacrol significantly down-regulate 7 functions, including Energy metabolism, Cell growth and death, and up-regulate 2 functions, including Carbohydrate metabolism and Membrane transport. The network analysis indicates that the correlation between gut bacteria also has been changed. In addition, the insecticidal activity results of carvacrol against L. dispar larvae with gut bacteria elimination showed that gut bacteria can reduce the insecticidal activity of carvacrol against L. dispar larvae.DiscussionThis study provides a theoretical foundation for understanding the role of gut bacteria in detoxifying plant toxins and conferring pesticide resistance.
{"title":"The impact of carvacrol on the larval gut bacterial structure and function of Lymantria dispar","authors":"Jing Yang, Yun-Ze Chen, Guo-Cai Zhang","doi":"10.3389/fmicb.2024.1417598","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1417598","url":null,"abstract":"IntroductionThe gut bacteria of insects play an important role in regulating their metabolism, immune system and metabolizing pesticides. Our previous results indicate that carvacrol has certain gastric toxic activity on <jats:italic>Lymantria dispar</jats:italic> larvae and affects their detoxification metabolism at the mRNA level. However, the impact of carvacrol on the gut bacteria of <jats:italic>L. dispar</jats:italic> larvae has been unclear.MethodsIn this study, the 16S rRNA sequencing technology was used to sequence and analyze the gut bacteria of the larvae which were exposed with sublethal concentration (0.297 mg/mL) and median lethal concentration (1.120 mg/mL), respectively.ResultsA total of 10 phyla, 16 classes, 47 orders, 72 families, 103 genera, and 135 species were obtained by using a 97% similarity cutoff level. The dominant bacterial phyla in the gut of the <jats:italic>L. dispar</jats:italic> larvae are <jats:italic>Firmicutes</jats:italic> and <jats:italic>Proteobacteria</jats:italic>. The treatment with carvacrol can significantly affect the structure of gut bacteria in the larvae of the <jats:italic>L. dispar</jats:italic>. At both doses, carvacrol can shift the dominant gut bacteria of the larvae from <jats:italic>Proteobacteria</jats:italic> to <jats:italic>Firmicutes</jats:italic>. At the genus level, two doses of carvacrol can significantly enhance the relative abundance of probiotic <jats:italic>Lactobacillus</jats:italic> in the gut of <jats:italic>L. dispar</jats:italic> larvae (<jats:italic>p</jats:italic> ≤ 0.01). Additionally, significant differences were observed among the five bacterial genera <jats:italic>Burkholderia-Caballeronia-Paraburkholderia</jats:italic>, <jats:italic>Anoxybacillus</jats:italic>, <jats:italic>Pelomonas</jats:italic>, <jats:italic>Mesorhizobium</jats:italic> (<jats:italic>p</jats:italic> ≤ 0.05). The analysis of <jats:italic>α</jats:italic>-diversity and <jats:italic>β</jats:italic>-diversity indicates that the treatment with carvacrol at two doses significantly affect the bacterial richness and diversity in the larvae. However, the results of functional classification prediction (PICRUSt) indicate that carvacrol significantly down-regulate 7 functions, including Energy metabolism, Cell growth and death, and up-regulate 2 functions, including Carbohydrate metabolism and Membrane transport. The network analysis indicates that the correlation between gut bacteria also has been changed. In addition, the insecticidal activity results of carvacrol against <jats:italic>L. dispar</jats:italic> larvae with gut bacteria elimination showed that gut bacteria can reduce the insecticidal activity of carvacrol against <jats:italic>L. dispar</jats:italic> larvae.DiscussionThis study provides a theoretical foundation for understanding the role of gut bacteria in detoxifying plant toxins and conferring pesticide resistance.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Growth factor receptor bound protein 7 (GRB7) is reportedly upregulated in human gastric cancer (GC), which is closely associated with tumor progression and prognosis. However, the mechanism underlying its dysregulation in GC remains poorly understood. In this study, we found that GRB7 overexpression was associated with Helicobacter pylori (H. pylori) infection. GC cells (AGS and MGC-803) infection assays revealed that this upregulation was mediated by the transcription factor STAT3, and activation of STAT3 by H. pylori promoted GRB7 expression in infected GC cells. Moreover, CagA, the key virulence factor of H. pylori, was found involved in STAT3-mediated GRB7 overexpression. The overexpressed GRB7 further promoted GC cell proliferation, migration, and invasion by activating ERK signaling. Mice infection was further used to investigate the action of GRB7. In H. pylori infection, GRB7 expression in mice gastric mucosa was elevated, and higher STAT3 and ERK activation were also detected. These results revealed GRB7-mediated pathogenesis in H. pylori infection, in which H. pylori activates STAT3, leading to increased GRB7 expression, then promotes activation of the ERK signal, and finally enhances malignant properties of infected cells. Our findings elucidate the role of GRB7 in H. pylori-induced gastric disorders, offering new prospects for the treatment and prevention of H. pylori-associated gastric carcinogenesis by targeting GRB7.
{"title":"GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection","authors":"Huilin Zhao, Si Chen, Xinfeng Bai, Jianhui Zhang, Shuzhen Liu, Zekun Sun, Xinying Cao, Jianping Wang, Ying Zhang, Boqing Li, Xiaofei Ji","doi":"10.3389/fmicb.2024.1469953","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1469953","url":null,"abstract":"Growth factor receptor bound protein 7 (GRB7) is reportedly upregulated in human gastric cancer (GC), which is closely associated with tumor progression and prognosis. However, the mechanism underlying its dysregulation in GC remains poorly understood. In this study, we found that GRB7 overexpression was associated with <jats:italic>Helicobacter pylori</jats:italic> (<jats:italic>H. pylori</jats:italic>) infection. GC cells (AGS and MGC-803) infection assays revealed that this upregulation was mediated by the transcription factor STAT3, and activation of STAT3 by <jats:italic>H. pylori</jats:italic> promoted GRB7 expression in infected GC cells. Moreover, CagA, the key virulence factor of <jats:italic>H. pylori</jats:italic>, was found involved in STAT3-mediated GRB7 overexpression. The overexpressed GRB7 further promoted GC cell proliferation, migration, and invasion by activating ERK signaling. Mice infection was further used to investigate the action of GRB7. In <jats:italic>H. pylori</jats:italic> infection, GRB7 expression in mice gastric mucosa was elevated, and higher STAT3 and ERK activation were also detected. These results revealed GRB7-mediated pathogenesis in <jats:italic>H. pylori</jats:italic> infection, in which <jats:italic>H. pylori</jats:italic> activates STAT3, leading to increased GRB7 expression, then promotes activation of the ERK signal, and finally enhances malignant properties of infected cells. Our findings elucidate the role of GRB7 in <jats:italic>H. pylori</jats:italic>-induced gastric disorders, offering new prospects for the treatment and prevention of <jats:italic>H. pylori</jats:italic>-associated gastric carcinogenesis by targeting GRB7.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1439267
Shuaibo Shao, Zhongwei Li, Yanqi Zhu, Yi Li, Yuanping Li, Linkun Wu, Christopher Rensing, Pumo Cai, Caihao Wang, Jianmin Zhang, Qisong Li
IntroductionIn mountainous tea plantations, which are the primary mode of tea cultivation in China, issues such as soil erosion and declining soil fertility are particularly severe. Although green manure cover is an effective agricultural measure for restoring soil fertility, its application in mountainous tea plantations has been relatively understudied.MethodsThis study investigated the effects of continuous green manure cover using the slope-protecting plant Ophiopogon japonicus on tea plant growth and soil microbial community structure. We implemented three treatments: 1 year of green manure coverage, 2 years of coverage, and a control, to study their effects on tea plant growth, soil physicochemical properties, and soil bacterial and fungal communities.ResultsResults demonstrate that green manure coverage significantly promote the growth of tea plants, enhanced organic matter and pH levels in soil, and various enzyme activities, including peroxidases and cellulases. Further functional prediction results indicate that green manure coverage markedly promoted several carbon cycling functions in soil microbes, including xylanolysis, cellulolysis, degradation of aromatic compounds, and saprotrophic processes. LEfSe analysis indicated that under green manure cover, the soil tends to enrich more beneficial microbial communities with degradation functions, such as Sphingomonas, Sinomonas, and Haliangium (bacteria), and Penicillium, Apiotrichum, and Talaromyce (fungi). In addition. Random forest and structural equation models indicated that carbon cycling, as a significant differentiating factor, has a significant promoting effect on tea plant growth.DiscussionIn the management practices of mountainous tea plantations, further utilizing slope-protecting plants as green manure can significantly influence the soil microbial community structure and function, enriching microbes involved in the degradation of organic matter and aromatic compounds, thereby positively impacting tea tree growth and soil nutrient levels.
{"title":"Green manure (Ophiopogon japonicus) cover promotes tea plant growth by regulating soil carbon cycling","authors":"Shuaibo Shao, Zhongwei Li, Yanqi Zhu, Yi Li, Yuanping Li, Linkun Wu, Christopher Rensing, Pumo Cai, Caihao Wang, Jianmin Zhang, Qisong Li","doi":"10.3389/fmicb.2024.1439267","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1439267","url":null,"abstract":"IntroductionIn mountainous tea plantations, which are the primary mode of tea cultivation in China, issues such as soil erosion and declining soil fertility are particularly severe. Although green manure cover is an effective agricultural measure for restoring soil fertility, its application in mountainous tea plantations has been relatively understudied.MethodsThis study investigated the effects of continuous green manure cover using the slope-protecting plant <jats:italic>Ophiopogon japonicus</jats:italic> on tea plant growth and soil microbial community structure. We implemented three treatments: 1 year of green manure coverage, 2 years of coverage, and a control, to study their effects on tea plant growth, soil physicochemical properties, and soil bacterial and fungal communities.ResultsResults demonstrate that green manure coverage significantly promote the growth of tea plants, enhanced organic matter and pH levels in soil, and various enzyme activities, including peroxidases and cellulases. Further functional prediction results indicate that green manure coverage markedly promoted several carbon cycling functions in soil microbes, including xylanolysis, cellulolysis, degradation of aromatic compounds, and saprotrophic processes. LEfSe analysis indicated that under green manure cover, the soil tends to enrich more beneficial microbial communities with degradation functions, such as <jats:italic>Sphingomonas</jats:italic>, <jats:italic>Sinomonas</jats:italic>, and <jats:italic>Haliangium</jats:italic> (bacteria), and <jats:italic>Penicillium</jats:italic>, <jats:italic>Apiotrichum</jats:italic>, and <jats:italic>Talaromyce</jats:italic> (fungi). In addition. Random forest and structural equation models indicated that carbon cycling, as a significant differentiating factor, has a significant promoting effect on tea plant growth.DiscussionIn the management practices of mountainous tea plantations, further utilizing slope-protecting plants as green manure can significantly influence the soil microbial community structure and function, enriching microbes involved in the degradation of organic matter and aromatic compounds, thereby positively impacting tea tree growth and soil nutrient levels.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1425898
Jinglei Zhang, Bo Wu, Guoliang Wang, Jinhong Zhang, Chunlin Jia
IntroductionIntercropping and soil properties both affect soil diazotrophic communities. However, the specific effects that alfalfa-maize intercropping has on diazotrophic networks and community diversity under different soil properties remain unclear.MethodsIn this study, we investigated the soil diazotrophic communities of two crop systems, alfalfa monoculture (AA) and alfalfa-maize intercropping (A/M), in two sites with similar climates but different soil properties (poor vs. average).Results and discussionThe diazotrophic network complexity and community diversity were higher at the site with poor soil than at the site with average soil (p < 0.05). Community structure also varied significantly between the sites with poor and average soil (p < 0.05). This divergence was mainly due to the differences in soil nitrogen, phosphorus, and organic carbon contents between the two sites. At the site with poor soil, the A/M system had lower diazotrophic diversity, lower network complexity and greater competition between diazotrophs than the AA system (p < 0.05) because intercropping intensified the soil phosphorus limitation under poor soil conditions. However, in the average soil, it was the A/M system that had an altered diazotrophic structure, with an increased abundance of 11 bacterial genera and a decreased abundance of three bacterial genera (p < 0.05).ConclusionOur results indicated that the effects of alfalfa-maize intercropping on diazotrophic communities were soil property-dependent.
引言间作和土壤特性都会影响土壤重氮营养群落。方法在本研究中，我们调查了在气候相似但土壤性质不同（贫瘠与一般）的两个地点，紫花苜蓿单作（AA）和紫花苜蓿-玉米间作（A/M）两种作物系统的土壤重氮群落。结果与讨论贫瘠土壤地块的重氮营养网络复杂性和群落多样性高于普通土壤地块（p &p;lt;0.05）。土壤贫瘠和土壤一般的地点之间的群落结构也有很大差异（p&p;lt; 0.05）。这种差异主要是由于两地土壤氮、磷和有机碳含量的不同造成的。在土壤贫瘠的地点，A/M 系统的重氮营养体多样性、网络复杂性和重氮营养体之间的竞争均低于 AA 系统（p &p;lt;0.05），这是因为在土壤贫瘠的条件下，间作加剧了土壤对磷的限制。结论我们的研究结果表明，苜蓿-玉米间作对重氮营养群落的影响取决于土壤性质。
{"title":"Responses of diazotrophic network structure and community diversity to alfalfa-maize intercropping are soil property-dependent","authors":"Jinglei Zhang, Bo Wu, Guoliang Wang, Jinhong Zhang, Chunlin Jia","doi":"10.3389/fmicb.2024.1425898","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1425898","url":null,"abstract":"IntroductionIntercropping and soil properties both affect soil diazotrophic communities. However, the specific effects that alfalfa-maize intercropping has on diazotrophic networks and community diversity under different soil properties remain unclear.MethodsIn this study, we investigated the soil diazotrophic communities of two crop systems, alfalfa monoculture (AA) and alfalfa-maize intercropping (A/M), in two sites with similar climates but different soil properties (poor vs. average).Results and discussionThe diazotrophic network complexity and community diversity were higher at the site with poor soil than at the site with average soil (<jats:italic>p</jats:italic> &lt; 0.05). Community structure also varied significantly between the sites with poor and average soil (<jats:italic>p</jats:italic> &lt; 0.05). This divergence was mainly due to the differences in soil nitrogen, phosphorus, and organic carbon contents between the two sites. At the site with poor soil, the A/M system had lower diazotrophic diversity, lower network complexity and greater competition between diazotrophs than the AA system (<jats:italic>p</jats:italic> &lt; 0.05) because intercropping intensified the soil phosphorus limitation under poor soil conditions. However, in the average soil, it was the A/M system that had an altered diazotrophic structure, with an increased abundance of 11 bacterial genera and a decreased abundance of three bacterial genera (<jats:italic>p</jats:italic> &lt; 0.05).ConclusionOur results indicated that the effects of alfalfa-maize intercropping on diazotrophic communities were soil property-dependent.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.3389/fmicb.2024.1465811
Freya D. R. Wencker, Seth E. Lyon, Ronald R. Breaker
An improved approach was developed for the genetic manipulation of the Gram-positive extremophile Halalkalibacterium halodurans (formerly called Bacillus halodurans). We describe an allelic replacement method originally developed for Staphylococcus aureus that allows the deletion, mutation, or insertion of genes without leaving markers or other genetic scars. In addition, a protocol for rapid in vitro plasmid methylation and transformation is presented. The combined methods allow the routine genetic manipulation of H. halodurans from initial transformation to the desired strain in 8 days. These methods improve H. halodurans as a model organism for the study of extremophiles.
{"title":"Improved methods for genetic manipulation of the alkaliphile Halalkalibacterium halodurans","authors":"Freya D. R. Wencker, Seth E. Lyon, Ronald R. Breaker","doi":"10.3389/fmicb.2024.1465811","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1465811","url":null,"abstract":"An improved approach was developed for the genetic manipulation of the Gram-positive extremophile <jats:italic>Halalkalibacterium halodurans</jats:italic> (formerly called <jats:italic>Bacillus halodurans</jats:italic>). We describe an allelic replacement method originally developed for <jats:italic>Staphylococcus aureus</jats:italic> that allows the deletion, mutation, or insertion of genes without leaving markers or other genetic scars. In addition, a protocol for rapid <jats:italic>in vitro</jats:italic> plasmid methylation and transformation is presented. The combined methods allow the routine genetic manipulation of <jats:italic>H. halodurans</jats:italic> from initial transformation to the desired strain in 8 days. These methods improve <jats:italic>H. halodurans</jats:italic> as a model organism for the study of extremophiles.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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