Fungal Genetics Reports最新文献

{"title":"Perspectives on genetic resources at the Fungal Genetics Stock Center.","authors":"K. McCluskey, M. Plamann","doi":"10.4148/1941-4765.1085","DOIUrl":"https://doi.org/10.4148/1941-4765.1085","url":null,"abstract":"The Fungal Genetics Stock Center has been banking and distributing resources for work with genetically characterized fungi since 1960. While most of the collection consists of strains of Neurospora, an NIH model filamentous fungus, the past fifteen years has seen the collection expand to include plant and human pathogenic fungi. The use of the resources in the collection has grown over the last 10 years as well, reflecting both a growth in research using standardized materials as well as the development of new materials through molecular genetic technology. This growth is not limited to newly deposited materials, however, and includes renewed interest in particular classes of strains with characteristics that were not recognized when they were originally deposited. One significant example is the use of strains carrying the osmotic-2 lesion in Neurospora crassa. This, and other utilization trends, underscores the need to provide strong support to the continued and expanded biobanking effort in the US. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol55/iss1/5","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"9 1","pages":"15-17"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73667365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using the 'Buller phenomenon' in experimental evolution studies of basidiomycetes","authors":"D. Aanen","doi":"10.4148/1941-4765.1084","DOIUrl":"https://doi.org/10.4148/1941-4765.1084","url":null,"abstract":"A. The standard life cycle of a heterothallic basidiomycete fungus. Sexual spores germinate (upper left) give rise to a haploid monokaryon. Two compatible monokaryons can fuse, upon which they reciprocally exchange nuclei, without cytoplasmic mixing (down left). This leads to the formation of the dikaryon (grey, right), all cells of which have two different nuclei, and which is a cytoplasmic mosaic. The dikaryon can produce mushrooms (schematically drawn on the grey dikaryon), the sexual fruiting bodies, where a short diploid stage is immediately followed by meiosis and sexual spore formation. The insert shows how the two nuclei in a dikaryotic cell are distributed over cells during cell division via the formation of clamp connections. B. The monokaryons exhibit two clearly distinct behaviors in a mating, accepting a nucleus, and donating a nucleus, which can be considered as female and male roles, respectively.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"2016 1","pages":"13-14"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86120134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspergillus Bibliography 2008","authors":"J. Clutterbuck","doi":"10.4148/1941-4765.1092","DOIUrl":"https://doi.org/10.4148/1941-4765.1092","url":null,"abstract":"This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection. This bibliography is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol55/iss1/12 Aspergillus bib 2008 ASPERGILLUS BIBLIOGRAPHY 2008 This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection. John Clutterbuck. Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK. Email: j.clutterbuck@bio.gla.ac.uk 1. Abe, K. & Gomi, K. 2008 Food products fermented by Aspergillus oryzae. Ch 25 in The Aspergilli: genomics, medical aspects, biotechnology, and research methods, ed. G.H. Goldman and S.A. Osmani, CRC Press, Boca Raton, pp 429-439 2. Abu Seadah, A.A. & El Shikh, M.E. 2008 RAPD typing of Aspergillus chevalieri, Aspergillus nidulans, Aspergillus tetrazonus (quadrilineatus) and their teleomorphs using 5'-d[AACGCGCAAC]-3' and 5'-d[CCCGTCAGCA]-3' primers. Mol. Biol. Repts. 35:89-95 3. Ahuja, M. & Punekar, N.S. 2008 Phosphinothricin resistance in Aspergillus niger and its utility as a selectable transformation marker. Fung. Genet. Biol. 45:1103-1110 4. Amillis, S., Hamari, Z., Roumelioti, K., Scazzocchio, C. & Diallinas, G. 2007 Regulation of expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans. Mol. Memb. Biol. 24:206-214 5. Andersen, M.R., Vongsangnak, W., Panagiotou, G., Salazar, M.P., Lehmann, L. & Nielsen, J. 2008 A trispecies Aspergillus microarray: comparative transcriptomics of three Aspergillus species. Proc. Natl. Acad. Sci, USA. 105:4387-4392 6. Araújo-Bazán, L., Fernández-Martínez, J., Ríos, V.M., Etxebeste, O., Albar, J.P., Peñalva, M.A. & Espeso, E.A. 2008 NapA and NapB are the Aspergillus nidulans Nap/SET family members and NapB is a nuclear protein specifically interacting with importin a. Fungal Genet. Biol. 45:278-291 7. Araújo-Bazán, L., Peñalva, M.A. & Espeso, E.A. 2008 Preferential localization of the endocytic internalization machinery to hyphal tips underlies polarization of the actin cytoskeleton in Aspergillus nidulans. Mol. Microbiol. 67:891-905 8. Atoui, A., Bao, D., Kaur, N., Grayburn, W.S. & Calvo, A.M. 2008 Aspergillus nidulans natural product biosynthesis is regulated by MpkB, a putative pheromone response mitogen-activated protein kinase. Appl. Env. Microbiol. 74:3596-3600 9. Baker, S.E. & Bennett, J.W. 2008 An overview of the genus Aspergillus. Ch 1 in The Asp","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"33 1","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76677602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complementation of un-16 and the development of a selectable marker for transformation of Neurospora crassa","authors":"K. McCluskey, S. Walker, Rachel L. Yedlin, David Madole, M. Plamann","doi":"10.4148/1941-4765.1097","DOIUrl":"https://doi.org/10.4148/1941-4765.1097","url":null,"abstract":"Although nearly sixty temperature-sensitive lesions have been mapped in Neurospora crassa, most of their functions have not been identified. These loci are called unknown (un). As part of an effort to identify the open reading frame associated with one of these, we undertook to walk to un-16 using the complementation of temperature-sensitivity as a selection. Cosmids complementing un-16 were identified and the un-16 gene was subcloned. DNA sequence analysis of un-16 revealed that it encodes the highly conserved S9 protein of the 40S ribosomal subunit. This gene has proven useful as a selectable marker and may provide a simple mechanism for the controlled alteration of protein synthesis in N. crassa.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"11 1","pages":"9-11"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74760392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"David Dexter Perkins (1919-2007)","authors":"A. Radford, N. Raju, D. Jacobson","doi":"10.4148/1941-4765.1099","DOIUrl":"https://doi.org/10.4148/1941-4765.1099","url":null,"abstract":"Obituary of David Dexter Perkins Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This obituary is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol54/iss1/5 Fungal Genetics Newsletter 54 In Press Obituaries David Dexter Perkins (1919-2007) David Perkins died on January 2, 2007 after a short illness. Two comprehensive obituaries, documenting the lives and scientific careers of David and his wife Dorothy Newmeyer Perkins (1922-2007) have been published elsewhere, and it is not our intention to duplicate those accounts, merely to commend them (Davis 2007, Genetics 175: 1-6; Raju 2007, J. Genetics, Vol. 86, in press). This is an account focusing specifically on David’s involvement with the Fungal Genetics Stock Center, Neurospora Newsletter and its successor, the Fungal Genetics Newsletter, over the past forty-five years. David was one of the five members of the committee that organized the first Neurospora Information Conference in 1961, the predecessor of the Fungal Genetics Conferences. A direct outgrowth of this first conference was the creation of the Neurospora Newsletter. David was always a strong supporter of the Newsletter, as a journal for the fast but refereed publication of short research notes, new linkage data, maps, FGSC stock lists relating to Neurospora, and subsequently, from 1986, to fungi in general. His own first item in the Newsletter was in volume 2, with a note on the favourable nature of the asci of bis x bis crosses for meiotic cytogenetics. Over the following four decades and more, David published eighty-five items in the Newsletter, covering research reports, novel techniques, new linkage data, regular updates of the complete linkage maps of Neurospora crassa, guidelines for gene nomenclature and obituaries for fellow pioneers in the sphere of Neurospora genetics. David was a tower of strength for the three editors of the Newsletter since its inception, Barbara Bachmann, Peter Russell and Matthew Sachs, not only as a contributor and as a reviewer of contributed papers but also in providing encouragement and moral support when needed. In addition to David’s Newsletter contributions, he played a major role in establishing and nurturing the Fungal Genetics Stock Center. Kevin McCluskey, the present curator of FGSC, compiled David’s major contributions to the stock center. Beginning in 1960, David deposited 3150 strains in the FGSC collection including strains 1-192. In 1999, he deposited most of his 3900 stains from wild collection. Thus it is not unusual to see the initials DDP associated with most of the Neurospora strains in the FGSC collection. David’s natural populations of Neurospora provide a rich source of variability that contributed to several major discoveries: heterokaryon incompatibility genes, transposable elements, senescence inducing plasmids, meiotic drive causing Spore killer elements etc. David was always frugal both in priv","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"65 1","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75119460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Yeast Plasmids that Confer Nourseothricin-Dihydrogen Sulfate and Hygromycin B Resistance in Neurospora crassa and Cryphonectria parasitica","authors":"R. P. Smith, Myron L Smith","doi":"10.4148/1941-4765.1098","DOIUrl":"https://doi.org/10.4148/1941-4765.1098","url":null,"abstract":"Two plasmids that were previously used with yeast, pRS41N and pRS41H, were found to confer clonNAT and hygromycin B resistance, respectively, in the filamentous fungi Neurospora crassa and Cryphonectria parasitica. These plasmids are suitable for routine cloning and for use in forcing heterokaryons and are available through the FGSC. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol54/iss1/4 12 Fungal Genetics Newsletter Two yeast plasmids that confer nourseothricin-dihydrogen sulfate and hygromycin B resistance in Neurospora crassa and Cryphonectria parasitica. Robert Phillip Smith and Myron L. Smith Nesbitt Biology Building, Department of Biology, Carleton University, Ottawa, Ontario, Canada. Fungal Genetics Newsletter 54:12-13 Two plasmids that were previously used with yeast, pRS41N and pRS41H, were found to confer clonNAT and hygromycin B resistance, respectively, in the filamentous fungi Neurospora crassa and Cryphonectria parasitica. These plasmids are suitable for routine cloning and for use in forcing heterokaryons and are available through the FGSC. Identification of novel resistance markers is important to further our ability to manipulate and characterize genetic elements in filamentous fungi. Hygromycin B (hygB) inhibits protein translation and has been used extensively as a selectable marker in filamentous fungi (Rao et al,, 1985). Alternative, affordable selectable markers are desirable for various applications, including for forcing heterokaryons. Previously, nourseothricin-dihydrogen sulfate (clonNAT) has been used as a selectable marker in fungi (Kück and Hoff, 2006). This aminoglycoside binds to ribosomes and results in inhibition and errors in protein synthesis (Cundliffe, 1989). Here we report that two vectors, pRS41N (clonNAT resistance) and pRS41H (hygB resistance), that were previously characterized in S. cerevisiae but not in filamentous fungi, are appropriate transformation vectors and suitable for forcing heterokaryons in Neurospora crassa and Cryphonectria parasitica. Figure 1. Yeast plasmids pRS41N and pRS41H that confer clonNAT and hygB resistance, respectively, to N. crassa and C. parasitica. Unique restriction sites in the multiple cloning site are indicated. Plasmids pRS41N and pRS41H (Figure 1) were derived from pRS416 as described in Taxis and Knop (2006). Resistance genes in both plasmids are driven by the TEF promoter from Ashbya gossypii, and S. cerevisiae CYC1 and ADH1 terminators are used in pRS41H and pRS41N, respectively. This contrasts to previously characterized vectors such as pCB1004 (Carroll et al., 1994) and pD-NAT1 (Kuck and Hoff, 2006) that use Aspergillus nidulans trpC promoter and terminator. Transformations with pRS41N and pRS41H were done with a PEG-mediated transformation protocol (Smith et al., 2000). Selection of N. crassa, pRS41N transfor","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"3 1","pages":"12-13"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77630618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of physical interactions by immunoprecipitation of FLAG- and HA-tagged proteins expressed at the his-3 locus in Neurospora crassa","authors":"Tsuyoshi Kawabata, H. Inoue","doi":"10.4148/1941-4765.1096","DOIUrl":"https://doi.org/10.4148/1941-4765.1096","url":null,"abstract":"Protein function is often regulated through interactions with other protein(s) or by post-translational modifications. To understand these mechanisms, it is useful to utilize antibodies. However, it is not always certain whether a good antibody can be made for this purpose. The use of epitope tags eliminates the troubles associated with raising antibodies. In this report, we present a method to detect interactions between proteins by using two types of epitope-tagged proteins, FLAGand HA-tagged proteins in Neurospora. These constructs were introduced at and expressed from the his-3 locus in different strains. To examine protein-protein interactions, heterokaryons between these strains were constructed. We conclude that this strategy is a useful tool to investigate protein function and protein interactions.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"58 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90806694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Norman H. Giles (1915-2006)","authors":"M. Case","doi":"10.4148/1941-4765.1100","DOIUrl":"https://doi.org/10.4148/1941-4765.1100","url":null,"abstract":"Obituary of Norman H. Giles Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This obituary is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol54/iss1/6 Fungal Genetics Newsletter 54 In Press Obituary Norman H. Giles (1915-2006) Norman H. Giles, 91, died on Oct. 16, 2006 at the Dartmouth-Hitchcock Medical Center from complication relating to a fall. He had recently moved from his home in Athens, GA to Norwich, VT to live with his daughter Annette Brown and her husband Arnie. He was born in Atlanta on August 6, 1915. He obtained his undergraduate degree from Emory University in 1937 and his Ph D degree from Harvard University in 1940. He married Dorothy Lunsford in 1939; she died in 1967. He subsequently married Doris Vos in 1969. He began his academic career in Botany at Yale University in 1941 and was appointed Eugene Higgins Professor of Genetics in 1961. He interrupted his time at Yale to work as principal biologist for three years at Oak Ridge National Laboratory from 1947-1950. He was elected to the National Academy of Sciences in 1966. Norman Giles was recognized as a pioneer in the fields of radiation cytology and genetics. His early works from 1939-1955 dealt with microsporogenesis and chromosome aberration studies in Tradescantia. This work was initiated at Harvard University and continued at Oak Ridge National Laboratory and at Yale University. In the mid 1940’s, his first studies with Neurospora crassa involved a reversion analysis of inositol mutants. This work followed the studies of Beadle and Tatum who dealt with reversion of nutritional mutants. In the early 1950’s, Norman became interested in the induction of mutations by UV and X-rays and in determining the nature of mutations blocking various biochemical pathways, e.g. pantothenic acid, adenine, methionine, histidine and aromatic biosynthesis. Subsequently, a number of important papers followed including intragenic complementation, gene conversion and an analysis of gene clusters. For example, complementation analysis of purple adenine mutants by Fred de Serres, Norman’s first graduate student, indicated that these mutants could be separated into two closely linked loci, ad-3A and ad-3B. Studies of allelic recombination at the pan-2 locus by Mary Case showed that gene conversion could occur at several different sites in one locus. Studies by Norma Nelson, Dow Woodward and C. W. H Partridge provided the first evidence for “allelic complementation” maps in vivo and complementation in vitro at the ad-4 locus. Extensive genetic and biochemical studies involved the arom mutants, a complex of five enzymes in the polyaromatic biosynthetic pathway. Later studies by others showed that the arom region encoded a dimer composed of two pentafunctional polypeptide chains. In studies of arom mutants, the absence of one class of mutants in the gene encoding biosynthetic dehydroquinase was missing. Further studie","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"5 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79776392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal Genetics Newsletter #54- 2007","authors":"M. Sachs","doi":"10.4148/1941-4765.1095","DOIUrl":"https://doi.org/10.4148/1941-4765.1095","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"18 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87271740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal Genetics Stock Center Catalogue of Strains","authors":"K. McCluskey, M. Plamann","doi":"10.4148/1941-4765.1118","DOIUrl":"https://doi.org/10.4148/1941-4765.1118","url":null,"abstract":"Catalogue of Strains, 11th edition, 2006, supplement to Fungal Genetics Newsletter No. 53. This catalogue contains lists of materials held by the Fungal Genetics Stock Center.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"23 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80094661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}