Pub Date : 2024-03-29DOI: 10.23946/2500-0764-2024-9-1-33-41
K. V. Marochko, N. Artymuk, A. V. Breus
Aim. To compare the effectiveness of Papanicolaou test, screening for oncogenic human papillomavirus (HPV) strains, and colposcopy in diagnosis of cervical squamous intraepithelial lesions and cervical cancer.Materials and Methods. We conducted a retrospective study which included 101 patients aged 21-65 years (mean age 36.9 ± 8.2 years). All patients underwent cytological examination, screening for 14 high-risk HPV types, and colposcopy. The prognostic value of the methods was calculated using standard formulas for determining the sensitivity, specificity, and prognostic value of positive and negative results. Cervical biopsy was accepted as the gold standard.Results. Among the examined women (n = 101), high-risk HPV types were detected in 78 women (77.2%). According to the results of cytological examination, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III, and cancer were detected in 24 (23.8%), 24 (23.8%), 22 (21.7%), and 2 (2%) cases, respectively. Abnormal colposcopy results of the 1st and 2nd degree were found in 33 (32.7%) and 51 (50.5%) women, respectively. The highest sensitivity in the diagnosis of low-grade squamous intraepithelial lesion was demonstrated by colposcopy (81.3%), whilst HPV testing and cytological examination had lower sensitivity values (75.0% and 58.3%, respectively). However, HPV testing had the highest sensitivity (85.7%) in diagnosis of high-grade squamous intraepithelial lesion and cervical cancer, whereas colposcopy and Papanicolaou test were behind (82.9% and 63.6%, respectively).Conclusion. Detection of high-risk HPV types has a high sensitivity in detecting both low- and highgrade squamous intraepithelial lesions and cervical cancer.
{"title":"Cervical Squamous Intraepithelial Lesions: A Comparison of Diagnostic Approaches","authors":"K. V. Marochko, N. Artymuk, A. V. Breus","doi":"10.23946/2500-0764-2024-9-1-33-41","DOIUrl":"https://doi.org/10.23946/2500-0764-2024-9-1-33-41","url":null,"abstract":"Aim. To compare the effectiveness of Papanicolaou test, screening for oncogenic human papillomavirus (HPV) strains, and colposcopy in diagnosis of cervical squamous intraepithelial lesions and cervical cancer.Materials and Methods. We conducted a retrospective study which included 101 patients aged 21-65 years (mean age 36.9 ± 8.2 years). All patients underwent cytological examination, screening for 14 high-risk HPV types, and colposcopy. The prognostic value of the methods was calculated using standard formulas for determining the sensitivity, specificity, and prognostic value of positive and negative results. Cervical biopsy was accepted as the gold standard.Results. Among the examined women (n = 101), high-risk HPV types were detected in 78 women (77.2%). According to the results of cytological examination, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III, and cancer were detected in 24 (23.8%), 24 (23.8%), 22 (21.7%), and 2 (2%) cases, respectively. Abnormal colposcopy results of the 1st and 2nd degree were found in 33 (32.7%) and 51 (50.5%) women, respectively. The highest sensitivity in the diagnosis of low-grade squamous intraepithelial lesion was demonstrated by colposcopy (81.3%), whilst HPV testing and cytological examination had lower sensitivity values (75.0% and 58.3%, respectively). However, HPV testing had the highest sensitivity (85.7%) in diagnosis of high-grade squamous intraepithelial lesion and cervical cancer, whereas colposcopy and Papanicolaou test were behind (82.9% and 63.6%, respectively).Conclusion. Detection of high-risk HPV types has a high sensitivity in detecting both low- and highgrade squamous intraepithelial lesions and cervical cancer.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"46 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-37-53
M. Khanova, A. Kutikhin, V. Matveeva, E. Velikanova, E. O. Krivkina, L. Antonova
Aim. To validate ECFC culture as a candidate culture for vascular tissue engineering using comparative analysis of the proteomic and gene expression profiles in comparison with cultures of human umbilical vein endothelial cells (HUVEC) and human coronary artery endothelial cells (HCAEC).Materials and Methods. ECFC culture was obtained by cultivating peripheral blood mononuclear cells of patients with coronary artery disease. Commercial HCAECs produced by Cell Applications, and HUVECs cultured according to the modified protocol of Jaffe were used as controls.The cells were lysed with TRIzol, and total RNA was isolated using a Purelink RNA Micro Scale Kit with concomitant DNase treatment. Next, rRNA depletion was carried out, followed by the creation of DNA libraries. DNA libraries were quantified using quantitative polymerase chain reaction on a CFX96 Touch Bio-Rad amplifier. DNA libraries were equimolarly mixed and sequenced on HiSeq 2000 (Illumina) with a paired-end reads of 2x125 nucleotides.Conventional western blotting was performed using pan-endothelial markers CD31, vWF, VEG-FR2/KDR, marker of endothelial progenitor cells CD34, markers of epithelial-mesenchymal transition Snail and Slug, and markers of endothelial specification: arterial HEY2, venous COUP-TFII and lymphatic LYVE1, VEGFR2. Dot blotting against 55 angiogenesis-related proteins was performed using Proteome Profiler Human Angiogenesis Array Kit in accordance with the manufacturer's protocol.Results. ECFC overexpresses markers of all three endothelial lineages (KDR, VWF, CD34, NRP2, FLT4 and LYVE1 compared to HCAEC; NOTCH4, DLL2) and LYVE1 compared to HUVEC. Proteomic profiling indicated ECFC as an intermediate population between HCAEC and HU-VEC in term of the expression of HEY2, LYVE1, VEGFR3, Snail and Slug. 261 DEGs were detected between ECFC and HUVEC, and 470 DEGs between ECFC and HCAEC.Conclusion. The gene expression profile of endothelial colony-forming cells corresponds to mature endothelial cells and indicates ECFC as an intermediate population between HCAEC and HUVEC. ECFC culture can be recommended for tissue vascular engineering.
{"title":"Colony-forming endothelial cells – candidate culture for tissue vascular engineering: the gene and proteomic profile","authors":"M. Khanova, A. Kutikhin, V. Matveeva, E. Velikanova, E. O. Krivkina, L. Antonova","doi":"10.23946/2500-0764-2023-8-4-37-53","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-37-53","url":null,"abstract":"Aim. To validate ECFC culture as a candidate culture for vascular tissue engineering using comparative analysis of the proteomic and gene expression profiles in comparison with cultures of human umbilical vein endothelial cells (HUVEC) and human coronary artery endothelial cells (HCAEC).Materials and Methods. ECFC culture was obtained by cultivating peripheral blood mononuclear cells of patients with coronary artery disease. Commercial HCAECs produced by Cell Applications, and HUVECs cultured according to the modified protocol of Jaffe were used as controls.The cells were lysed with TRIzol, and total RNA was isolated using a Purelink RNA Micro Scale Kit with concomitant DNase treatment. Next, rRNA depletion was carried out, followed by the creation of DNA libraries. DNA libraries were quantified using quantitative polymerase chain reaction on a CFX96 Touch Bio-Rad amplifier. DNA libraries were equimolarly mixed and sequenced on HiSeq 2000 (Illumina) with a paired-end reads of 2x125 nucleotides.Conventional western blotting was performed using pan-endothelial markers CD31, vWF, VEG-FR2/KDR, marker of endothelial progenitor cells CD34, markers of epithelial-mesenchymal transition Snail and Slug, and markers of endothelial specification: arterial HEY2, venous COUP-TFII and lymphatic LYVE1, VEGFR2. Dot blotting against 55 angiogenesis-related proteins was performed using Proteome Profiler Human Angiogenesis Array Kit in accordance with the manufacturer's protocol.Results. ECFC overexpresses markers of all three endothelial lineages (KDR, VWF, CD34, NRP2, FLT4 and LYVE1 compared to HCAEC; NOTCH4, DLL2) and LYVE1 compared to HUVEC. Proteomic profiling indicated ECFC as an intermediate population between HCAEC and HU-VEC in term of the expression of HEY2, LYVE1, VEGFR3, Snail and Slug. 261 DEGs were detected between ECFC and HUVEC, and 470 DEGs between ECFC and HCAEC.Conclusion. The gene expression profile of endothelial colony-forming cells corresponds to mature endothelial cells and indicates ECFC as an intermediate population between HCAEC and HUVEC. ECFC culture can be recommended for tissue vascular engineering.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"142 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139453004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-73-84
E. E. Sadovnikov, N. Potseluev, O. Barbarash, E. B. Brusina
Aim. To identify the epidemiological features of HAIs in all patients admitted for surgery from 2018 to 2022. in a cardiac surgery hospital for the implementation of a risk-based prevention strategy.Materials and Methods. A descriptive retrospective epidemiological study of the HAI epidemic process was performed from 2018 to 2022. in patients of a large cardiac surgery hospital (n = 6179). Stratified indicators were calculated. To display unknown relationships and make a forecast, Fourier spectral analysis was performed, followed by the use of artificial intelligence technology - neural networks. The STATISTICA Automated Neural Networks (SANN) tool was used, as well as the StatTech v. 3.0.5.Results. The average rate of HAIs incidence over a 5-year period was 4.22 per 1000 patient days. We revealed decreasing trend of HAIs. Incidence of HCAI cardiopulmonary bypass surgery (CBS) was 3 times higher than without CBS (4.68 and 1.51 per 1000 patient-days, respectively). Fourier analysis revealed 10, 20, 30 cyclicity due to the dominant Klebsiella pneumoniae without the same time-series for other pathogens. The technology of neural network modeling did not reveal neural networks suitable for describing the forecast. Klebsiella pneumoniae showed properties typical of the hospital population and caused 35.49% of all cases of HAIs, had multidrug resistance to antibiotics in 74.45% of cases, with more than half of the strains having extended resistance, and 10.21% were pan-resistant. Acinetobacter baumanii also showed high epidemic activity, causing almost a fifth of all cases of HAIs, although its antimicrobial resistance characteristics were less pronounced than those of Klebsiella pneumoniae.Conclusion. The epidemiological characteristics of the epidemic process of HCAI is one of the mandatory components of risk identification. The identified features of the dynamics of the epidemic process of HCAI in a cardiac surgery hospital, risk groups and time, the structure and characteristics of the microbiota should be taken into account in the HCAI risk management system.
{"title":"Healthcare-associated infections in cardiac surgery: epidemiological features","authors":"E. E. Sadovnikov, N. Potseluev, O. Barbarash, E. B. Brusina","doi":"10.23946/2500-0764-2023-8-4-73-84","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-73-84","url":null,"abstract":"Aim. To identify the epidemiological features of HAIs in all patients admitted for surgery from 2018 to 2022. in a cardiac surgery hospital for the implementation of a risk-based prevention strategy.Materials and Methods. A descriptive retrospective epidemiological study of the HAI epidemic process was performed from 2018 to 2022. in patients of a large cardiac surgery hospital (n = 6179). Stratified indicators were calculated. To display unknown relationships and make a forecast, Fourier spectral analysis was performed, followed by the use of artificial intelligence technology - neural networks. The STATISTICA Automated Neural Networks (SANN) tool was used, as well as the StatTech v. 3.0.5.Results. The average rate of HAIs incidence over a 5-year period was 4.22 per 1000 patient days. We revealed decreasing trend of HAIs. Incidence of HCAI cardiopulmonary bypass surgery (CBS) was 3 times higher than without CBS (4.68 and 1.51 per 1000 patient-days, respectively). Fourier analysis revealed 10, 20, 30 cyclicity due to the dominant Klebsiella pneumoniae without the same time-series for other pathogens. The technology of neural network modeling did not reveal neural networks suitable for describing the forecast. Klebsiella pneumoniae showed properties typical of the hospital population and caused 35.49% of all cases of HAIs, had multidrug resistance to antibiotics in 74.45% of cases, with more than half of the strains having extended resistance, and 10.21% were pan-resistant. Acinetobacter baumanii also showed high epidemic activity, causing almost a fifth of all cases of HAIs, although its antimicrobial resistance characteristics were less pronounced than those of Klebsiella pneumoniae.Conclusion. The epidemiological characteristics of the epidemic process of HCAI is one of the mandatory components of risk identification. The identified features of the dynamics of the epidemic process of HCAI in a cardiac surgery hospital, risk groups and time, the structure and characteristics of the microbiota should be taken into account in the HCAI risk management system.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"131 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139453448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-24-36
I. M. Ordiyants, D. S. Novginov, Z. Zyukina, A. M. Khachatryan, S. Titov
Aim. To develop a method for noninvasive diagnosis of external genital endometriosis based on plasma microRNA concentrations.Materials and Methods. 80 women of reproductive age who were admitted to the gynecological department for routine laparoscopy were retrospectively examined, according to the results of which and histological examination, the patients were divided into 2 groups: the main group — 54 patients with laparoscopically and histologically confirmed external genital endometriosis (EGE); the control group — 26 patients without EGE. Before laparoscopy, a blood sample was taken from all patients for a molecular-biological study of the expression of 10 microRNAs: miR-183, miR-125b, miR-126, miR-16, miR-15a, miR-200a, miR-20a, miR-21, miR-222 and miR-29b. Identification of the studied and normalizing RNAs (U6 RNA and 103a microRNA) was performed according to the method of Chen et al. The presented values of the expression of the studied microRNAs are given in the form of 2-ΔCt. The expression ratio is given in the form of 2-ΔCt (main)/2-ΔCt (control), if the expression in the group of patients with endometriosis exceeded that in the control group, and in the form of 2-ΔCt (control)/2-ΔCt (main), if vice versa.Results. Comparison of the expression of 10 mi-croRNAs between the two groups revealed statistically significant differences only in miR-183: its expression in patients with EGE was statistically 1.5 times higher than that in women of the control group (p=0.017).We have not detected a difference in the expression of mir-200a, while according to other researchers, representatives of the mir-200 family are among the most frequent whose expression changes with endometriosis. MIR-16 expression also did not differ statistically among the patients we examined, whereas a group of American colleagues revealed its increase in patients with endometriosis and with endometriosis-associated ovarian tumors. We found no difference in mir-21 expression. The results of other researchers are contradictory: some found its increase in endometrioid cysts compared with eutopic endometrium, an increase in the epithelium of the fallopian tubes with their endometriosis compared with unaffected; others did not reveal a difference between the eutopic endometrium of endometriosis patients and healthy women, but showed a decrease in expression in peritoneal foci and foci of deep infiltrative endometriosis compared with eutopic endometrium.The expression of mir-222 was reduced in the patients we examined with endometriosis, which goes against the existing ideas about the pro-oncogenic role of this microRNA. An increase in its expression in cancer of the stomach, bladder, liver, lungs, breast, endometrium, ovaries is described. At the same time, the oncosuppressive effect of mir-222 is also known in prostate cancer, squamous cell carcinoma of the oral cavity.Conclusion. Taking into account the revealed statistically significant difference in microRNA expression by
{"title":"Non-invasive diagnostics of endometriosis based on plasma miRNA expression","authors":"I. M. Ordiyants, D. S. Novginov, Z. Zyukina, A. M. Khachatryan, S. Titov","doi":"10.23946/2500-0764-2023-8-4-24-36","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-24-36","url":null,"abstract":"Aim. To develop a method for noninvasive diagnosis of external genital endometriosis based on plasma microRNA concentrations.Materials and Methods. 80 women of reproductive age who were admitted to the gynecological department for routine laparoscopy were retrospectively examined, according to the results of which and histological examination, the patients were divided into 2 groups: the main group — 54 patients with laparoscopically and histologically confirmed external genital endometriosis (EGE); the control group — 26 patients without EGE. Before laparoscopy, a blood sample was taken from all patients for a molecular-biological study of the expression of 10 microRNAs: miR-183, miR-125b, miR-126, miR-16, miR-15a, miR-200a, miR-20a, miR-21, miR-222 and miR-29b. Identification of the studied and normalizing RNAs (U6 RNA and 103a microRNA) was performed according to the method of Chen et al. The presented values of the expression of the studied microRNAs are given in the form of 2-ΔCt. The expression ratio is given in the form of 2-ΔCt (main)/2-ΔCt (control), if the expression in the group of patients with endometriosis exceeded that in the control group, and in the form of 2-ΔCt (control)/2-ΔCt (main), if vice versa.Results. Comparison of the expression of 10 mi-croRNAs between the two groups revealed statistically significant differences only in miR-183: its expression in patients with EGE was statistically 1.5 times higher than that in women of the control group (p=0.017).We have not detected a difference in the expression of mir-200a, while according to other researchers, representatives of the mir-200 family are among the most frequent whose expression changes with endometriosis. MIR-16 expression also did not differ statistically among the patients we examined, whereas a group of American colleagues revealed its increase in patients with endometriosis and with endometriosis-associated ovarian tumors. We found no difference in mir-21 expression. The results of other researchers are contradictory: some found its increase in endometrioid cysts compared with eutopic endometrium, an increase in the epithelium of the fallopian tubes with their endometriosis compared with unaffected; others did not reveal a difference between the eutopic endometrium of endometriosis patients and healthy women, but showed a decrease in expression in peritoneal foci and foci of deep infiltrative endometriosis compared with eutopic endometrium.The expression of mir-222 was reduced in the patients we examined with endometriosis, which goes against the existing ideas about the pro-oncogenic role of this microRNA. An increase in its expression in cancer of the stomach, bladder, liver, lungs, breast, endometrium, ovaries is described. At the same time, the oncosuppressive effect of mir-222 is also known in prostate cancer, squamous cell carcinoma of the oral cavity.Conclusion. Taking into account the revealed statistically significant difference in microRNA expression by","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"19 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-115-123
M. A. Akimenko, O. A. Voronova, M. S. Alkhuseyn-Kuliaginova, A. B. Alnikin, N. A. Kornienko, M. Dodokhova, M. V. Gulyan, I. Kotieva
Despite the wide arsenal of chemotherapeutic agents, the search and study of new compounds with an alleged antitumor effect is relevant. Morphological diagnostics of pathological processes occurring under the action of pharmacologically active substances is the most important component of preclinical research of compounds with an alleged antitumor effect. It is advisable to use information about the possible cytotoxic effect of candidates for antitumor drugs using an immunohistochemical method for studying organs and systems of experimental animals at different stages of the development of the tumor process by indirect markers of tumor progression activity. Morphological examination of parenchymal organs and tumor tissue in the dynamics of the development of malignant neoplasm is more informative and evidence-based than biochemical research. The aim of the study is to conduct a comparative analysis of markers of tumor process activity for more effective use of morphological and immunohistochemical research methods in the preclinical study of compounds with suspected antitumor activity to assess the prospects for their use with the detection of tumor process activity. The literature search was carried out using the Scopus, Web of Science, PubMed and eLIBRARY databases. The paper presents an overview of current molecular biological markers for assessing the activity of the malignant process in the experiment: Transforming Growth Factor beta 1 (TGF-β1), Ki-67, Tumor necrosis factor alpha (TNF-α), p53, Poly-ADP-ribose polymerase 1 (PARP-1) and Anti-8-Hydroxy-2'-deoxyguanosine (8-OHdG), beta III Tubulin, p120 Catenin, Beta Actin. The listed markers are indirect and can be used in a single mode only for screening studies of antitumor and antimetastatic activity in which a large number of compounds are sorted according to the principle of effectiveness. When conducting an in-depth study of the pharmacological activity of the leader compounds it is necessary to perform a comprehensive immunohistochemical study. Our analysis of the literature data confirms the importance of selecting optimal, sensitive, economically feasible and affordable markers, which in turn leads to the improvement of diagnostic panels and their standardization to simplify their transition into clinical practice.
尽管化疗药物种类繁多，但寻找和研究据称具有抗肿瘤作用的新化合物仍然具有现实意义。对药理活性物质作用下发生的病理过程进行形态学诊断，是对据称具有抗肿瘤作用的化合物进行临床前研究的最重要组成部分。最好使用免疫组化方法，通过肿瘤进展活动的间接标志物来研究实验动物器官和系统在肿瘤进程发展的不同阶段可能产生的细胞毒性作用。在恶性肿瘤的动态发展过程中，对实质器官和肿瘤组织进行形态学检查比生化研究更有参考价值和依据。本研究的目的是对肿瘤进程活性的标志物进行比较分析，以便在具有可疑抗肿瘤活性的化合物的临床前研究中更有效地使用形态学和免疫组化研究方法，评估其在肿瘤进程活性检测中的应用前景。本文使用 Scopus、Web of Science、PubMed 和 eLIBRARY 数据库进行文献检索。本文概述了目前用于评估实验中恶性过程活性的分子生物学标记物：转化生长因子 beta 1 (TGF-β1)、Ki-67、肿瘤坏死因子α (TNF-α)、p53、聚-ADP-核糖聚合酶 1 (PARP-1) 和抗-8-羟基-2'-脱氧鸟苷 (8-OHdG)、β III 管蛋白、p120 连环素、β 肌动蛋白。所列标记物均为间接标记物，只能以单一模式用于抗肿瘤和抗转移活性的筛选研究，根据有效性原则对大量化合物进行排序。在对领军化合物的药理活性进行深入研究时，有必要进行全面的免疫组化研究。我们对文献数据的分析证实了选择最佳、灵敏、经济可行且负担得起的标记物的重要性，这反过来又会导致诊断面板的改进及其标准化，从而简化其过渡到临床实践的过程。
{"title":"The possibilities of immunohistochemistry for assessing the pathogenetic mechanisms of action of compounds with a suspected antitumor effect. Part I. General indicators of the process activity","authors":"M. A. Akimenko, O. A. Voronova, M. S. Alkhuseyn-Kuliaginova, A. B. Alnikin, N. A. Kornienko, M. Dodokhova, M. V. Gulyan, I. Kotieva","doi":"10.23946/2500-0764-2023-8-4-115-123","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-115-123","url":null,"abstract":"Despite the wide arsenal of chemotherapeutic agents, the search and study of new compounds with an alleged antitumor effect is relevant. Morphological diagnostics of pathological processes occurring under the action of pharmacologically active substances is the most important component of preclinical research of compounds with an alleged antitumor effect. It is advisable to use information about the possible cytotoxic effect of candidates for antitumor drugs using an immunohistochemical method for studying organs and systems of experimental animals at different stages of the development of the tumor process by indirect markers of tumor progression activity. Morphological examination of parenchymal organs and tumor tissue in the dynamics of the development of malignant neoplasm is more informative and evidence-based than biochemical research. The aim of the study is to conduct a comparative analysis of markers of tumor process activity for more effective use of morphological and immunohistochemical research methods in the preclinical study of compounds with suspected antitumor activity to assess the prospects for their use with the detection of tumor process activity. The literature search was carried out using the Scopus, Web of Science, PubMed and eLIBRARY databases. The paper presents an overview of current molecular biological markers for assessing the activity of the malignant process in the experiment: Transforming Growth Factor beta 1 (TGF-β1), Ki-67, Tumor necrosis factor alpha (TNF-α), p53, Poly-ADP-ribose polymerase 1 (PARP-1) and Anti-8-Hydroxy-2'-deoxyguanosine (8-OHdG), beta III Tubulin, p120 Catenin, Beta Actin. The listed markers are indirect and can be used in a single mode only for screening studies of antitumor and antimetastatic activity in which a large number of compounds are sorted according to the principle of effectiveness. When conducting an in-depth study of the pharmacological activity of the leader compounds it is necessary to perform a comprehensive immunohistochemical study. Our analysis of the literature data confirms the importance of selecting optimal, sensitive, economically feasible and affordable markers, which in turn leads to the improvement of diagnostic panels and their standardization to simplify their transition into clinical practice.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"63 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-16-23
E. N. Kravchenko, E. V. Lautenschleger
Aim. To study the clinical and anamnestic characteristics of patients with endometrial hyperplasia (EH) and endometrial polyps (PE).Material and Methods. a retrospective study was conducted, which included 267 sick women of reproductive and perimenopausal age who sought gynecological care at «Euromed» in 2022-23, who, before receiving the examination results, were diagnosed with N92 - heavy, frequent and irregular menstruation. Depending on the results of the pathomorphological examination, the patients were divided into 3 groups: group A included 89 patients with GE; group B – 99 women with PE; Group B (control) included 79 patients in whom endometrial pathology was excluded.Results. Among all women who came to the clinic with heavy menstrual bleeding, 33.3% had GE, 37.1% had PE, 29.6% had BUN-O ovulatory dysfunction. GE without atypia was detected in 72 (80.9%) cases of group A, GE with atypia – in 7 (19.1%), in group B PE was diagnosed in 91 (91.9%) cases, PE with atypia – in 28 (8.1%).In group B, in 22 (27.8%) cases, the endometrium was identified in the proliferation phase, in 57 (72.2%) cases, the endometrium was identified in the phase of incomplete secretion.Conclusion. Women with GE are characterized by early menarche, absence of pregnancies and childbirth during life associated with the woman’s choice, contraception, endocrine diseases, obesity, polycystic ovary syndrome, ovarian tumors, heavy bleeding with clots during menstruation, menstruation for more than 8 days, chronic iron deficiency anemia. Women with endometrial polyps are characterized by arterial hypertension, uterine fibroids, adenomyosis, infertility, dysmenorrhea, chronic endometritis, a history of cervicitis and vaginitis; clinical manifestations include intermenstrual bleeding.
材料和方法研究子宫内膜增生症（EH）和子宫内膜息肉（PE）患者的临床和病理特征。材料和方法：该研究是一项回顾性研究，包括 2022-23 年期间在 "Euromed "就诊的 267 名育龄和围绝经期患病妇女，她们在接受检查前被诊断为 N92--月经过多、过频和月经不调。根据病理形态学检查结果，患者被分为三组：A组包括89名GE患者；B组--99名PE妇女；B组（对照组）包括79名排除子宫内膜病变的患者。在所有因大量月经出血前来就诊的妇女中，33.3%患有子宫内膜异位症，37.1%患有子宫内膜息肉，29.6%患有BUN-O排卵功能障碍。在 A 组中，72 例（80.9%）发现子宫内膜异位症，7 例（19.1%）发现子宫内膜异位症；在 B 组中，91 例（91.9%）确诊为子宫内膜异位症，28 例（8.1%）确诊为子宫内膜异位症；在 B 组中，22 例（27.8%）发现子宫内膜处于增殖期，57 例（72.2%）发现子宫内膜处于不完全分泌期。患有子宫内膜息肉的妇女的特点是月经初潮早、一生中没有怀孕和生育，这与妇女的选择、避孕、内分泌疾病、肥胖、多囊卵巢综合征、卵巢肿瘤、月经期间大量出血并伴有血块、月经超过 8 天、慢性缺铁性贫血有关。患有子宫内膜息肉的妇女主要表现为动脉高血压、子宫肌瘤、子宫腺肌症、不孕、痛经、慢性子宫内膜炎、宫颈炎和阴道炎病史；临床表现为月经间期出血。
{"title":"Endometrial hyperplasia and endometrial polyps: clinical and anamnestic characteristics of women","authors":"E. N. Kravchenko, E. V. Lautenschleger","doi":"10.23946/2500-0764-2023-8-4-16-23","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-16-23","url":null,"abstract":"Aim. To study the clinical and anamnestic characteristics of patients with endometrial hyperplasia (EH) and endometrial polyps (PE).Material and Methods. a retrospective study was conducted, which included 267 sick women of reproductive and perimenopausal age who sought gynecological care at «Euromed» in 2022-23, who, before receiving the examination results, were diagnosed with N92 - heavy, frequent and irregular menstruation. Depending on the results of the pathomorphological examination, the patients were divided into 3 groups: group A included 89 patients with GE; group B – 99 women with PE; Group B (control) included 79 patients in whom endometrial pathology was excluded.Results. Among all women who came to the clinic with heavy menstrual bleeding, 33.3% had GE, 37.1% had PE, 29.6% had BUN-O ovulatory dysfunction. GE without atypia was detected in 72 (80.9%) cases of group A, GE with atypia – in 7 (19.1%), in group B PE was diagnosed in 91 (91.9%) cases, PE with atypia – in 28 (8.1%).In group B, in 22 (27.8%) cases, the endometrium was identified in the proliferation phase, in 57 (72.2%) cases, the endometrium was identified in the phase of incomplete secretion.Conclusion. Women with GE are characterized by early menarche, absence of pregnancies and childbirth during life associated with the woman’s choice, contraception, endocrine diseases, obesity, polycystic ovary syndrome, ovarian tumors, heavy bleeding with clots during menstruation, menstruation for more than 8 days, chronic iron deficiency anemia. Women with endometrial polyps are characterized by arterial hypertension, uterine fibroids, adenomyosis, infertility, dysmenorrhea, chronic endometritis, a history of cervicitis and vaginitis; clinical manifestations include intermenstrual bleeding.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"59 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-54-64
Y. Kudryavtseva, A. Y. Kanonykina, N. A. Efremova, V. A. Koshelev
When performing operations on the abdominal and thoracic organs, the main share of postoperative complications is represented by the formation of adhesions or the development of an infectious process, which leads to a decrease in the quality of life of patients, the need for reoperation and often causes deaths. A solution to this problem can be the use of intraoperative biodegradable anti-adhesion membranes that have their own antibacterial activity.Aim. Development of polymer anti-adhesion membranes with their own anti-inflammatory and antibacterial activity, assessment of their biocompatibility and biodegradation in in vivo experimentsMaterials and Methods. The membranes are made by electrospinning from a composition of biodegradable polymers: polylactide-co-glycolide copolymer (50:50) and polylactide-co-glycolide (85:15). To impart antibacterial properties to the membrane, the antibiotic Tigacil was added to the polymer solution in various dosages - 0.125; 0.25 and 0.5 mg/ml polymer solution. The antibacterial activity of the membranes in vivo was assessed. The physical and mechanical properties were studied and the surface structure of the membranes was assessed using a scanning electron microscope. Biocompatibility and dynamics of biodegradation were assessed in vivo by implantation into laboratory animals (rats) for periods of 14 days, 1, 2 and 3 months, followed by histological examination of explanted samples.Results. Polymer membranes made by electrospinning, without the inclusion of Tigacil, consist of threads whose thickness was 1.63 microns (1.422.85 microns); when Tigacil is included in the fiber composition, the fiber thickness decreases to 1.2 microns (0.977 - 1.89 pm), while the threads are more densely and orderly located. The strength and elasticity modulus of membranes with Tigacil are almost 2 times higher than those of samples without the inclusion of the drug. The maximum antibacterial effect was achieved at a Tigacil dosage of 0.5 mg/ml - the zone of inhibition of Staphylococcus aureus at a Tigacil concentration of 0.125 mg/ ml was 146%, 0.25 mg/ml - 152% and at a concentration of 0.5 mg/ml - 11 .5 mm 177%. The inclusion of Tigacil led to a decrease in the rate of biodegradation of samples in vivo. The samples underwent biodegradation without signs of acute and chronic inflammation.Conclusion. The inclusion of Tigacil in the membrane gives it antibacterial properties, and the optimal concentration of Tigacil was 0.5 mg/ml of the polymer solution. The inclusion of Tigacil in the polymer composition affects the morphology of the membranes, increases the strength and elastic modulus, which led to a decrease in the rate of degradation when implanted subcutaneously in rats. The absence of signs of inflammation confirms the biocompatibility of the developed membranes.
{"title":"Biocompatibility and features of degradation of polymer anti-adjection membranes with antibacterial activity","authors":"Y. Kudryavtseva, A. Y. Kanonykina, N. A. Efremova, V. A. Koshelev","doi":"10.23946/2500-0764-2023-8-4-54-64","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-54-64","url":null,"abstract":"When performing operations on the abdominal and thoracic organs, the main share of postoperative complications is represented by the formation of adhesions or the development of an infectious process, which leads to a decrease in the quality of life of patients, the need for reoperation and often causes deaths. A solution to this problem can be the use of intraoperative biodegradable anti-adhesion membranes that have their own antibacterial activity.Aim. Development of polymer anti-adhesion membranes with their own anti-inflammatory and antibacterial activity, assessment of their biocompatibility and biodegradation in in vivo experimentsMaterials and Methods. The membranes are made by electrospinning from a composition of biodegradable polymers: polylactide-co-glycolide copolymer (50:50) and polylactide-co-glycolide (85:15). To impart antibacterial properties to the membrane, the antibiotic Tigacil was added to the polymer solution in various dosages - 0.125; 0.25 and 0.5 mg/ml polymer solution. The antibacterial activity of the membranes in vivo was assessed. The physical and mechanical properties were studied and the surface structure of the membranes was assessed using a scanning electron microscope. Biocompatibility and dynamics of biodegradation were assessed in vivo by implantation into laboratory animals (rats) for periods of 14 days, 1, 2 and 3 months, followed by histological examination of explanted samples.Results. Polymer membranes made by electrospinning, without the inclusion of Tigacil, consist of threads whose thickness was 1.63 microns (1.422.85 microns); when Tigacil is included in the fiber composition, the fiber thickness decreases to 1.2 microns (0.977 - 1.89 pm), while the threads are more densely and orderly located. The strength and elasticity modulus of membranes with Tigacil are almost 2 times higher than those of samples without the inclusion of the drug. The maximum antibacterial effect was achieved at a Tigacil dosage of 0.5 mg/ml - the zone of inhibition of Staphylococcus aureus at a Tigacil concentration of 0.125 mg/ ml was 146%, 0.25 mg/ml - 152% and at a concentration of 0.5 mg/ml - 11 .5 mm 177%. The inclusion of Tigacil led to a decrease in the rate of biodegradation of samples in vivo. The samples underwent biodegradation without signs of acute and chronic inflammation.Conclusion. The inclusion of Tigacil in the membrane gives it antibacterial properties, and the optimal concentration of Tigacil was 0.5 mg/ml of the polymer solution. The inclusion of Tigacil in the polymer composition affects the morphology of the membranes, increases the strength and elastic modulus, which led to a decrease in the rate of degradation when implanted subcutaneously in rats. The absence of signs of inflammation confirms the biocompatibility of the developed membranes.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"3 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139453203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-8-15
L. M. Nebyshynets, E. N. Grudnitskaya, S. L. Voskresensky, E. N. Volkovets
Aim. To study of serum magnesium in women with a history of adverse pregnancy outcomes.Materials and Methods. 74 women of child-bearing age, subject to voluntary informed consent, participated in a prospective cohort study. The main group (n = 31) included non-pregnant women who had a history of unfavorable gestation outcomes: premature birth, spontaneous miscarriage, habitual miscarriage, undeveloped pregnancy. The comparison group (n = 43) consisted of non-pregnant women with a history of two or more urgent deliveries. In all women, in preparation for pregnancy, the content of serum magnesium was determined by colorimetric method with xylidine blue.Results. 77.6% of women with a burdened obstetric history of miscarriage (the main group) had various menstrual cycle disorders (FIGO, 2018). When analyzing the levels of magnesium in the blood serum of the examined women, it turned out that the obtained indicators in women of both groups corresponded to the established reference range of normal values of serum magnesium levels. At the same time, in the women of the main group, the average magnesium content in the blood serum was significantly lower and was closer to the lower limit of the range of normal values, and in the comparison group – in the middle of the range of normal values: 0.719 (0.672-0.767) mmol/l and 0.844 (0.778-0.922) mmol/l, respectively, p<0.001.The optimal threshold value of the serum magnesium index in the study was 0.796 mmol/l with the sensitivity and specificity of the proposed prognostic model of 80.6% and 81.4%, respectively.Conclusion. Determination of the level of magnesium in the blood serum of women at the stage of pre-pregnancy counseling is of particular importance. Despite the fact that all the examined women had serum magnesium levels within the range of its normal values, significantly lower serum magnesium levels were found in women with adverse pregnancy outcomes in the anamnesis, which necessitates prescribing magnesium-containing medications to such patients at the stage of pregnancy planning. For the prevention of miscarriage within the framework of preconception preparation with serum magnesium values ≤0.796 mmol/l, the intake of organic magnesium salts is recommended.
{"title":"Serum magnesium in women with a history of adverse pregnancy outcomes","authors":"L. M. Nebyshynets, E. N. Grudnitskaya, S. L. Voskresensky, E. N. Volkovets","doi":"10.23946/2500-0764-2023-8-4-8-15","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-8-15","url":null,"abstract":"Aim. To study of serum magnesium in women with a history of adverse pregnancy outcomes.Materials and Methods. 74 women of child-bearing age, subject to voluntary informed consent, participated in a prospective cohort study. The main group (n = 31) included non-pregnant women who had a history of unfavorable gestation outcomes: premature birth, spontaneous miscarriage, habitual miscarriage, undeveloped pregnancy. The comparison group (n = 43) consisted of non-pregnant women with a history of two or more urgent deliveries. In all women, in preparation for pregnancy, the content of serum magnesium was determined by colorimetric method with xylidine blue.Results. 77.6% of women with a burdened obstetric history of miscarriage (the main group) had various menstrual cycle disorders (FIGO, 2018). When analyzing the levels of magnesium in the blood serum of the examined women, it turned out that the obtained indicators in women of both groups corresponded to the established reference range of normal values of serum magnesium levels. At the same time, in the women of the main group, the average magnesium content in the blood serum was significantly lower and was closer to the lower limit of the range of normal values, and in the comparison group – in the middle of the range of normal values: 0.719 (0.672-0.767) mmol/l and 0.844 (0.778-0.922) mmol/l, respectively, p<0.001.The optimal threshold value of the serum magnesium index in the study was 0.796 mmol/l with the sensitivity and specificity of the proposed prognostic model of 80.6% and 81.4%, respectively.Conclusion. Determination of the level of magnesium in the blood serum of women at the stage of pre-pregnancy counseling is of particular importance. Despite the fact that all the examined women had serum magnesium levels within the range of its normal values, significantly lower serum magnesium levels were found in women with adverse pregnancy outcomes in the anamnesis, which necessitates prescribing magnesium-containing medications to such patients at the stage of pregnancy planning. For the prevention of miscarriage within the framework of preconception preparation with serum magnesium values ≤0.796 mmol/l, the intake of organic magnesium salts is recommended.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"46 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-101-114
G. R. Khasanova, M. S. Muzaffarova
The “aging” of the population increased the importance of researches in the field of the epidemiology of chronic diseases, including Alzheimer's disease (AD) -the most common cause of dementia in the population.Aim. The role of potential risk factors for AD through a systematic review and meta-analysis. The “aging” of the population has updated research in the field of the epidemiology of chronic diseases, incl. Alzheimer's disease (AD) is the most common cause of dementia in the population. The aim of the study was to assess the role of potential risk factors for AD through a systematic review and meta-analysis.Materials and Methods. Using the electronic databases PubMed, Scopus, E-library, a search was made for articles in Russian and English, published from 1995 to 2022. In accordance with the clinical question, using the PECO formula, papers were selected in which the authors investigated the role of various risk factors in groups with and without AD. The study was carried out in accordance with the international guidelines for writing systematic reviews and meta-analyses "PRISMA". Study quality was analyzed using the Newcastle-Ottawa scale for cohort and case-control studies. The degree of heterogeneity was assessed using the chi-square test and the I2 coefficient. Publication bias was analyzed using a funnel plot. We used the software Review Manager 5.3 and Microsoft Office Excel 2010.Results. Initially, 3197 articles were retrieved from the databases; After screening and eligibility analysis, 17 studies were included in the me-ta-analysis (11 case-control studies and 6 cohort studies). Totally, these publications included data from 134,732 people with a confirmed diagnosis of AD and 1,058,143 respondents without AD (control group). According to the results of the meta-analysis, significant risk factors were: heredity (odds ratio (OR) 1.82; 95% confidence interval (95% CI) 1.66-1.99), arterial hypertension (OR 1.65; 95% CI 1.29-2.13), hypercholesterolemia (OR 1.25; 95% CI 1.13-1.38), obesity (OR 1.13; 95% CI 1.09-1.17), presence of diabetes mellitus 2 type (OR 1.36; 95%; CI 1.15-1.62), low level of education (OR 1.61; 95% CI 1.18-2.18), depression (OR 1.35; 95% CI 1.03-1.76). There was no relationship with alcohol consumption, smoking, a history of myocardial infarction and / or coronary heart disease, a history of acute cerebrovascular accident, insomnia, female gender, traumatic brain injury.Conclusion. The conducted meta-analysis allowed to obtain confirmation of the role of various potential risk factors for AD; at the same time, many of them are modifiable and are associated with metabolic disorders, which can probably be involved into the process of accumulation and deposition of beta-amyloid in the cells of the nervous system. Continued research on this issue could contribute to the development of prognostic scales and personalized recommendations for the prevention of this currently incurable disease.
人口的 "老龄化 "增加了慢性疾病流行病学领域研究的重要性，其中包括阿尔茨海默病（AD）--人口中最常见的痴呆症病因。通过系统综述和荟萃分析研究阿尔茨海默病潜在风险因素的作用。人口的 "老龄化 "更新了慢性病流行病学领域的研究，其中阿尔茨海默病（AD）是人群中最常见的痴呆症病因。本研究旨在通过系统回顾和荟萃分析，评估潜在风险因素对阿尔茨海默病的作用。使用电子数据库 PubMed、Scopus 和 E-library，搜索 1995 年至 2022 年期间发表的俄文和英文文章。根据临床问题，使用 PECO 公式筛选出作者研究了各种风险因素在 AD 患者和非 AD 患者中的作用的论文。研究按照国际系统综述和荟萃分析撰写指南 "PRISMA "进行。研究质量采用纽卡斯尔-渥太华量表对队列研究和病例对照研究进行分析。异质性程度采用卡方检验和 I2 系数进行评估。采用漏斗图分析发表偏倚。我们使用了Review Manager 5.3软件和Microsoft Office Excel 2010。最初，我们从数据库中检索到了 3197 篇文章；经过筛选和资格分析，17 项研究被纳入了 me-ta 分析（11 项病例对照研究和 6 项队列研究）。这些研究共纳入了 134,732 名确诊为注意力缺失症的患者和 1,058,143 名未确诊为注意力缺失症的受访者（对照组）的数据。根据荟萃分析的结果，重要的风险因素有：遗传（几率比（OR）1.82；95% 置信区间（95% CI）1.66-1.99）、动脉高血压（OR 1.65；95% CI 1.29-2.13）、高胆固醇血症（OR 1.25；95% CI 1.13-1.38）、肥胖（OR 1.13；95% CI 1.09-1.17）、患有 2 型糖尿病（OR 1.36；95%；CI 1.15-1.62）、受教育程度低（OR 1.61；95% CI 1.18-2.18）、抑郁（OR 1.35；95% CI 1.03-1.76）。与饮酒、吸烟、心肌梗塞和/或冠心病病史、急性脑血管意外病史、失眠、女性性别、脑外伤等均无关系。所进行的荟萃分析证实了各种潜在风险因素对注意力缺失症的作用；同时，其中许多风险因素是可以改变的，并与代谢紊乱有关，而代谢紊乱很可能参与了β-淀粉样蛋白在神经系统细胞中的积累和沉积过程。对这一问题的继续研究将有助于制定预后量表和个性化建议，以预防这一目前无法治愈的疾病。
{"title":"Risk factors for the alzheimer's disease. Systematic review and meta-analysis","authors":"G. R. Khasanova, M. S. Muzaffarova","doi":"10.23946/2500-0764-2023-8-4-101-114","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-101-114","url":null,"abstract":"The “aging” of the population increased the importance of researches in the field of the epidemiology of chronic diseases, including Alzheimer's disease (AD) -the most common cause of dementia in the population.Aim. The role of potential risk factors for AD through a systematic review and meta-analysis. The “aging” of the population has updated research in the field of the epidemiology of chronic diseases, incl. Alzheimer's disease (AD) is the most common cause of dementia in the population. The aim of the study was to assess the role of potential risk factors for AD through a systematic review and meta-analysis.Materials and Methods. Using the electronic databases PubMed, Scopus, E-library, a search was made for articles in Russian and English, published from 1995 to 2022. In accordance with the clinical question, using the PECO formula, papers were selected in which the authors investigated the role of various risk factors in groups with and without AD. The study was carried out in accordance with the international guidelines for writing systematic reviews and meta-analyses \"PRISMA\". Study quality was analyzed using the Newcastle-Ottawa scale for cohort and case-control studies. The degree of heterogeneity was assessed using the chi-square test and the I2 coefficient. Publication bias was analyzed using a funnel plot. We used the software Review Manager 5.3 and Microsoft Office Excel 2010.Results. Initially, 3197 articles were retrieved from the databases; After screening and eligibility analysis, 17 studies were included in the me-ta-analysis (11 case-control studies and 6 cohort studies). Totally, these publications included data from 134,732 people with a confirmed diagnosis of AD and 1,058,143 respondents without AD (control group). According to the results of the meta-analysis, significant risk factors were: heredity (odds ratio (OR) 1.82; 95% confidence interval (95% CI) 1.66-1.99), arterial hypertension (OR 1.65; 95% CI 1.29-2.13), hypercholesterolemia (OR 1.25; 95% CI 1.13-1.38), obesity (OR 1.13; 95% CI 1.09-1.17), presence of diabetes mellitus 2 type (OR 1.36; 95%; CI 1.15-1.62), low level of education (OR 1.61; 95% CI 1.18-2.18), depression (OR 1.35; 95% CI 1.03-1.76). There was no relationship with alcohol consumption, smoking, a history of myocardial infarction and / or coronary heart disease, a history of acute cerebrovascular accident, insomnia, female gender, traumatic brain injury.Conclusion. The conducted meta-analysis allowed to obtain confirmation of the role of various potential risk factors for AD; at the same time, many of them are modifiable and are associated with metabolic disorders, which can probably be involved into the process of accumulation and deposition of beta-amyloid in the cells of the nervous system. Continued research on this issue could contribute to the development of prognostic scales and personalized recommendations for the prevention of this currently incurable disease.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23946/2500-0764-2023-8-4-65-72
A. D. Chubarova, M. S. Turchaninova, N. V. Gogadze, E. A. Vilms, E. Shcherba
Aim. Assessment of the content of trans-fatty acids in foods consumed by the population of the Omsk region and the values of their actual consumption.Materials and Methods. The results of laboratory studies of food products from the following groups were analyzed: fast food (hamburgers, french fries, chips); confectionery; oils, fats; meat products; dairy products (n=438). In product samples, the mass fraction of trans-fatty acid isomers was determined by gas chromatography. An assessment was made of the actual nutrition and consumption of trans fats by the inhabitants of the region using the method of analyzing the frequency of food consumption (n=441).Results. The median content of trans fats in the main food groups was 0.67% of total fat, which does not exceed the values recommended by WHO. There was a decrease in the content of trans-isomers in food products from 2.7% in the period 2016−2017. up to 1.0% in the period 2020−2021 However, there remains a significant health risk associated with the consumption of trans-fatty acids, due to the high proportion of samples exceeding the recommended levels (24.5±2.1%), to a greater extent the risk was determined by the consumption of confectionery. The median consumption of TFAs in the study population was 1.16 g, which is in line with the recommended norms. The proportion of people with excess recommended consumption was 10.2%.Conclusion. The results indicate an improvement in the situation related to the consumption of trans fats in 2016-2021 due to stricter requirements for their content in food products. It is necessary to conduct further research related to the assessment of the content of trans-isomers of fatty acids in the diet of various population groups to identify risk groups and priority food products - sources of trans fats.
{"title":"Hygienic assessment of the consumption of trans fatty acids by the population of the Omsk region","authors":"A. D. Chubarova, M. S. Turchaninova, N. V. Gogadze, E. A. Vilms, E. Shcherba","doi":"10.23946/2500-0764-2023-8-4-65-72","DOIUrl":"https://doi.org/10.23946/2500-0764-2023-8-4-65-72","url":null,"abstract":"Aim. Assessment of the content of trans-fatty acids in foods consumed by the population of the Omsk region and the values of their actual consumption.Materials and Methods. The results of laboratory studies of food products from the following groups were analyzed: fast food (hamburgers, french fries, chips); confectionery; oils, fats; meat products; dairy products (n=438). In product samples, the mass fraction of trans-fatty acid isomers was determined by gas chromatography. An assessment was made of the actual nutrition and consumption of trans fats by the inhabitants of the region using the method of analyzing the frequency of food consumption (n=441).Results. The median content of trans fats in the main food groups was 0.67% of total fat, which does not exceed the values recommended by WHO. There was a decrease in the content of trans-isomers in food products from 2.7% in the period 2016−2017. up to 1.0% in the period 2020−2021 However, there remains a significant health risk associated with the consumption of trans-fatty acids, due to the high proportion of samples exceeding the recommended levels (24.5±2.1%), to a greater extent the risk was determined by the consumption of confectionery. The median consumption of TFAs in the study population was 1.16 g, which is in line with the recommended norms. The proportion of people with excess recommended consumption was 10.2%.Conclusion. The results indicate an improvement in the situation related to the consumption of trans fats in 2016-2021 due to stricter requirements for their content in food products. It is necessary to conduct further research related to the assessment of the content of trans-isomers of fatty acids in the diet of various population groups to identify risk groups and priority food products - sources of trans fats.","PeriodicalId":12493,"journal":{"name":"Fundamental and Clinical Medicine","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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