L. Ky, P. V. Huu, N. X. Quang, T. Hai, M. Q. Hoan, N. BacHai, N. D. Phúc
{"title":"Research Article Use of transoral CO2 laser microsurgery for treatment of cancerous neoplasms of the larynx in Vietnam","authors":"L. Ky, P. V. Huu, N. X. Quang, T. Hai, M. Q. Hoan, N. BacHai, N. D. Phúc","doi":"10.4238/GMR18734","DOIUrl":"https://doi.org/10.4238/GMR18734","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"20 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70930927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Pscheidt, R. C. Lemos, J. C. Souza, V. B. Oliveira, A. M. Souza, J. Pádua
The species Nicotiana tabacum, known as tobacco, is one of the crops with the highest economic value in the world among non-food species. Since 2000, Brazil has become the world‟s second largest tobacco producer. Brazilian production is mainly concentrated in the South region. The main tobacco types are produced in Brazil, such as Dark, which, despite representing a small percentage of the total production in Brazil, is economically important because of a high added value. Until now the only available cultivar is a line that despite having a desirable alkaloid content, has low productivity. In an effort to help improve production, we estimated the heterosis of diallel crosses involving tobacco lines of the Dark tobacco type. A complete diallel cross was made involving 10 lines from the British American Tobacco company breeding program. The 45 hybrid combinations, together with the 10 parental lines and nine other commercial controls, were evaluated at four sites in Southern Brazil, using a triple lattice experimental design (8 x 8). The characteristics evaluated included: green leaf mass (Productivity YLD), and total alkaloid content (ALK), and the selection index was obtained by the sum of standardized variables (SSV), considering a weight of 70% for YLD and 30% for ALK. Estimated correlation between YLD and
{"title":"Research Article Feasibility of using tobacco hybrids of the Dark tobacco type","authors":"A. Pscheidt, R. C. Lemos, J. C. Souza, V. B. Oliveira, A. M. Souza, J. Pádua","doi":"10.4238/gmr18929","DOIUrl":"https://doi.org/10.4238/gmr18929","url":null,"abstract":"The species Nicotiana tabacum, known as tobacco, is one of the crops with the highest economic value in the world among non-food species. Since 2000, Brazil has become the world‟s second largest tobacco producer. Brazilian production is mainly concentrated in the South region. The main tobacco types are produced in Brazil, such as Dark, which, despite representing a small percentage of the total production in Brazil, is economically important because of a high added value. Until now the only available cultivar is a line that despite having a desirable alkaloid content, has low productivity. In an effort to help improve production, we estimated the heterosis of diallel crosses involving tobacco lines of the Dark tobacco type. A complete diallel cross was made involving 10 lines from the British American Tobacco company breeding program. The 45 hybrid combinations, together with the 10 parental lines and nine other commercial controls, were evaluated at four sites in Southern Brazil, using a triple lattice experimental design (8 x 8). The characteristics evaluated included: green leaf mass (Productivity YLD), and total alkaloid content (ALK), and the selection index was obtained by the sum of standardized variables (SSV), considering a weight of 70% for YLD and 30% for ALK. Estimated correlation between YLD and","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70934176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BLAST is a genomic local alignment search tool used to identify homology between genotypes and possible orthologous genes. In vitro microsatellite transferability is a strategy to enable or increase species molecular fingerprinting, but it is dependent on PCR technique. An initial in silico step using BLAST for transferability can be helpful to save resources in pre-selecting markers more likely to amplify. We aligned and transfered SSR sequences from Psidium guajava to Eucalyptus globulus using BLAST. Twenty-three SSR clone sequences from P. guajava (query) were retrieved from the NCBI website and aligned against the whole genome of E. globulus (subject) using a cut-off e-value<1.00e −20 . Another 140 loci retrieved from the GuavaMap project were analyzed using as parameters e-values<1.7 and a maximum distance of 300 nucleotides between forward and reverse sequences. All loci were analyzed using BLASTN with MEGABLAST optimization. DNA extraction of four eucalypt trees was performed with the 2x CTAB protocol containing a sorbitol initial step. Validation of the SSR selected via BLASTN was performed by PCR reactions with 12 loci (seven selected on Blast hits and five without hits) and posterior visualization on polyacrylamide gel. Nine out of 23 microsatellite loci were transferable in the in silico, with a mean identity of 87%. With regard to the GuavaMap microsatellite loci, only three showed significant
{"title":"Research Article In silico microsatellite transferability from Psidium guajava to Eucalyptus globulus validated by PCR","authors":"F. Medeiros, Cátia Santos, A. Costa","doi":"10.4238/gmr18985","DOIUrl":"https://doi.org/10.4238/gmr18985","url":null,"abstract":"BLAST is a genomic local alignment search tool used to identify homology between genotypes and possible orthologous genes. In vitro microsatellite transferability is a strategy to enable or increase species molecular fingerprinting, but it is dependent on PCR technique. An initial in silico step using BLAST for transferability can be helpful to save resources in pre-selecting markers more likely to amplify. We aligned and transfered SSR sequences from Psidium guajava to Eucalyptus globulus using BLAST. Twenty-three SSR clone sequences from P. guajava (query) were retrieved from the NCBI website and aligned against the whole genome of E. globulus (subject) using a cut-off e-value<1.00e −20 . Another 140 loci retrieved from the GuavaMap project were analyzed using as parameters e-values<1.7 and a maximum distance of 300 nucleotides between forward and reverse sequences. All loci were analyzed using BLASTN with MEGABLAST optimization. DNA extraction of four eucalypt trees was performed with the 2x CTAB protocol containing a sorbitol initial step. Validation of the SSR selected via BLASTN was performed by PCR reactions with 12 loci (seven selected on Blast hits and five without hits) and posterior visualization on polyacrylamide gel. Nine out of 23 microsatellite loci were transferable in the in silico, with a mean identity of 87%. With regard to the GuavaMap microsatellite loci, only three showed significant","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70935252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Evaluating the pain relief effects after caesarean delivery by ultrasound-guided transversus abdominis plane (tap) block in Hanoi Obstetrics and Gynecology Hospital, Vietnam","authors":"N. Lam, N. Khanh, N. D. Anh","doi":"10.4238/GMR18739","DOIUrl":"https://doi.org/10.4238/GMR18739","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"6 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70931341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Aclasta effectively treats osteoporosis in patients with femoral head fractures","authors":"V. T. Toan, B. V. Anh","doi":"10.4238/GMR18744","DOIUrl":"https://doi.org/10.4238/GMR18744","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"20 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70931724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Oro, R. M. Costa, E. A. Britta, C. V. Nakamura, L. F. Ribeiro, M. A. Fernandez
{"title":"Research Article Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) larva integument infected by Bombyx mori nucleopolyhedrovirus","authors":"A. Oro, R. M. Costa, E. A. Britta, C. V. Nakamura, L. F. Ribeiro, M. A. Fernandez","doi":"10.4238/GMR18758","DOIUrl":"https://doi.org/10.4238/GMR18758","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"20 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70932672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region","authors":"V. N. Santos, R. Gama, C. Santos","doi":"10.4238/GMR18778","DOIUrl":"https://doi.org/10.4238/GMR18778","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"20 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70932849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. C. Pereira, J. O. Costa, C. Costa, N. S. Lima, J. Barros, D. Bento, A. Reis, R. Santos
{"title":"Research Article New insights of miRNAs dysregulation in the molecular pathological basis of neurodegenerative sclerosis: A systematic review","authors":"J. C. Pereira, J. O. Costa, C. Costa, N. S. Lima, J. Barros, D. Bento, A. Reis, R. Santos","doi":"10.4238/gmr18843","DOIUrl":"https://doi.org/10.4238/gmr18843","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"42 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70933337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Ribeiro, D. Silva, S. Rovaris, J. Gonçalves, S. Carbonell, A. F. Chiorato
Common bean yield is directly related to climate conditions, and water deficit is one of the main limiting factors. One way of getting around this problem is increasing the frequency of alleles favorable to drought tolerance by the recurrent selection method. We estimated the morphophysiological and agronomic gains achieved in two recurrent selection cycles for drought tolerance and evaluated the genetic potential of the progenies obtained in each cycle. The first recurrent selection cycle was obtained by intercrossing 10 genotypes. This cycle was followed by physiological, morphophysiological, and agronomic evaluations, resulting in selection of 17 progenies. The second cycle was obtained by intercrossing the 17 selected progenies, followed by the same evaluations, resulting in 20 selected progenies. A randomized block experimental design was used for both selection cycles, with split plots and three replications. The plots consisted of two water treatments (irrigated and water deficit), and the subplots consisted of the progenies under evaluation. To select the progenies and estimate the genetic parameters, only the treatment under water deficit was considered, in randomized blocks with three replications. Irrigation was suspended at the R5 stage. Under these conditions, the following traits were evaluated: stomatal conductance, leaf temperature, relative
{"title":"Research Article Recurrent selection to obtain drought-tolerant common bean progenies","authors":"T. Ribeiro, D. Silva, S. Rovaris, J. Gonçalves, S. Carbonell, A. F. Chiorato","doi":"10.4238/gmr18902","DOIUrl":"https://doi.org/10.4238/gmr18902","url":null,"abstract":"Common bean yield is directly related to climate conditions, and water deficit is one of the main limiting factors. One way of getting around this problem is increasing the frequency of alleles favorable to drought tolerance by the recurrent selection method. We estimated the morphophysiological and agronomic gains achieved in two recurrent selection cycles for drought tolerance and evaluated the genetic potential of the progenies obtained in each cycle. The first recurrent selection cycle was obtained by intercrossing 10 genotypes. This cycle was followed by physiological, morphophysiological, and agronomic evaluations, resulting in selection of 17 progenies. The second cycle was obtained by intercrossing the 17 selected progenies, followed by the same evaluations, resulting in 20 selected progenies. A randomized block experimental design was used for both selection cycles, with split plots and three replications. The plots consisted of two water treatments (irrigated and water deficit), and the subplots consisted of the progenies under evaluation. To select the progenies and estimate the genetic parameters, only the treatment under water deficit was considered, in randomized blocks with three replications. Irrigation was suspended at the R5 stage. Under these conditions, the following traits were evaluated: stomatal conductance, leaf temperature, relative","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70933393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Selection of genomic regions and genes associated with adaptation and fertility traits in two Colombian creole cattle breeds","authors":"C. D. Leon, R. Martínez, J. F. Rocha, A. Darghan","doi":"10.4238/gmr18882","DOIUrl":"https://doi.org/10.4238/gmr18882","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70933421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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