Pub Date : 2018-07-05DOI: 10.1128/genomeA.00636-18
Nicolas E Gaultier, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Carmon Kee, Balakrishnan N V Premkrishnan, Cassie E Heinle, Serene B Y Lim, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster
The thermophilic bacterium Geobacillus thermoleovorans was isolated from a tropical air sample collected in Singapore. The genome was sequenced on the PacBio RS II platform and consists of one chromosome with 3.6 Mb and one plasmid with 75 kb. The genome comprises 3,509 protein-coding genes, 88 tRNAs, and 27 rRNAs.
{"title":"Genome Sequence of Geobacillus thermoleovorans SGAir0734, Isolated from Singapore Air.","authors":"Nicolas E Gaultier, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Carmon Kee, Balakrishnan N V Premkrishnan, Cassie E Heinle, Serene B Y Lim, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster","doi":"10.1128/genomeA.00636-18","DOIUrl":"https://doi.org/10.1128/genomeA.00636-18","url":null,"abstract":"<p><p>The thermophilic bacterium <i>Geobacillus thermoleovorans</i> was isolated from a tropical air sample collected in Singapore. The genome was sequenced on the PacBio RS II platform and consists of one chromosome with 3.6 Mb and one plasmid with 75 kb. The genome comprises 3,509 protein-coding genes, 88 tRNAs, and 27 rRNAs.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00636-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00643-18
Irvan Luhung, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Nicolas E Gaultier, Balakrishnan N V Premkrishnan, Cassie E Heinle, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster
Pantoea ananatis SGAir0210 was isolated from outdoor air collected in Singapore. The genome was assembled from long reads generated by single-molecule real-time sequencing complemented with short reads. The genome size was approximately 4.81 Mb, with 4,303 protein-coding genes, 80 tRNAs, and 22 rRNAs identified.
{"title":"Genome Sequence of Pantoea ananatis SGAir0210, Isolated from Outdoor Air in Singapore.","authors":"Irvan Luhung, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Nicolas E Gaultier, Balakrishnan N V Premkrishnan, Cassie E Heinle, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster","doi":"10.1128/genomeA.00643-18","DOIUrl":"https://doi.org/10.1128/genomeA.00643-18","url":null,"abstract":"<p><p><i>Pantoea ananatis</i> SGAir0210 was isolated from outdoor air collected in Singapore. The genome was assembled from long reads generated by single-molecule real-time sequencing complemented with short reads. The genome size was approximately 4.81 Mb, with 4,303 protein-coding genes, 80 tRNAs, and 22 rRNAs identified.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00643-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lactobacillus paracasei EG9 is a strain isolated from well-ripened cheese and accelerates free amino acid production during cheese ripening. Its complete genome sequence was determined using the PacBio RS II platform, revealing a single circular chromosome of 2,927,257 bp, a G+C content of 46.59%, and three plasmids.
{"title":"Complete Genome Sequence of Lactobacillus paracasei EG9, a Strain Accelerating Free Amino Acid Production during Cheese Ripening.","authors":"Yui Asahina, Akino Shiroma, Kazuma Nakano, Hinako Tamotsu, Noriko Ashimine, Misuzu Shinzato, Maiko Minami, Makiko Shimoji, Tetsuhiro Nakanishi, Shun Ohki, Kuniko Teruya, Kazuhito Satou, Miho Kobayashi, Tatsuro Hagi, Naoko Moriya, Chise Suzuki, Atsushi Tajima, Masaru Nomura, Takashi Hirano","doi":"10.1128/genomeA.00627-18","DOIUrl":"https://doi.org/10.1128/genomeA.00627-18","url":null,"abstract":"<p><p><i>Lactobacillus paracasei</i> EG9 is a strain isolated from well-ripened cheese and accelerates free amino acid production during cheese ripening. Its complete genome sequence was determined using the PacBio RS II platform, revealing a single circular chromosome of 2,927,257 bp, a G+C content of 46.59%, and three plasmids.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00627-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00546-18
Hue Thi Kim Vu, Matthew J Stasiewicz, Soottawat Benjakul, Kitiya Vongkamjan
We report here the complete genome sequences of three Listeria phages (PSU-VKH-LP019, PSU-VKH-LP040, and PSU-VKH-LP041), which were newly induced from lysogenic isolates of Listeria monocytogenes from seafood and a seafood processing environment in Thailand. The three phages show circularly permuted double-stranded DNA genomes with sizes of 38.6, 39.6, and 48.3 kb.
{"title":"Genome Sequences of Listeria Phages Induced from Lysogenic Isolates of Listeria monocytogenes from Seafood and a Seafood Processing Environment in Thailand.","authors":"Hue Thi Kim Vu, Matthew J Stasiewicz, Soottawat Benjakul, Kitiya Vongkamjan","doi":"10.1128/genomeA.00546-18","DOIUrl":"https://doi.org/10.1128/genomeA.00546-18","url":null,"abstract":"<p><p>We report here the complete genome sequences of three <i>Listeria</i> phages (PSU-VKH-LP019, PSU-VKH-LP040, and PSU-VKH-LP041), which were newly induced from lysogenic isolates of <i>Listeria monocytogenes</i> from seafood and a seafood processing environment in Thailand. The three phages show circularly permuted double-stranded DNA genomes with sizes of 38.6, 39.6, and 48.3 kb.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00546-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00572-18
Evgeniya Volkova, Andriyan Grinev, Rafaelle Fares-Gusmao, Caren Chancey, Maria Rios
We report here the complete genome sequences of two Zika virus strains (FSS13025 and PRVABC59) used for formulation of CBER/FDA RNA reference reagents and lot release panels for use with nucleic acid technology (NAT) testing.
{"title":"Complete Genome Sequences of Zika Virus Strains Used for the Formulation of CBER/FDA RNA Reference Reagents and Lot Release Panels for Nucleic Acid Technology Testing.","authors":"Evgeniya Volkova, Andriyan Grinev, Rafaelle Fares-Gusmao, Caren Chancey, Maria Rios","doi":"10.1128/genomeA.00572-18","DOIUrl":"https://doi.org/10.1128/genomeA.00572-18","url":null,"abstract":"<p><p>We report here the complete genome sequences of two Zika virus strains (FSS13025 and PRVABC59) used for formulation of CBER/FDA RNA reference reagents and lot release panels for use with nucleic acid technology (NAT) testing.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00572-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00488-18
Ana Isabela Lopes Sales, Guilherme Paier Milanez, Leandro C Nascimento, Camila Pinheiro do Carmo, Fernanda Luz Paulino da Costa, Gonçalo Amarante Guimarães Pereira, Roberto Martinez, Marcelo Brocchi
Draft genomes of three Salmonella enterica 4,[5],12:i:- (STi) strains isolated from human infections were obtained using Illumina sequencing. They were negative for the fljBA operon but positive for hin, and k-mer analyses revealed their identity as S. enterica 4,[5],12:i:- 08-1736 and S Typhimurium. A draft S Typhimurium sequence is described for comparison.
{"title":"Draft Genome Sequences of Three Salmonella enterica Serovar 4,[5],12:i:- Strains and One S. enterica Serovar Typhimurium Strain, Isolated in Brazil.","authors":"Ana Isabela Lopes Sales, Guilherme Paier Milanez, Leandro C Nascimento, Camila Pinheiro do Carmo, Fernanda Luz Paulino da Costa, Gonçalo Amarante Guimarães Pereira, Roberto Martinez, Marcelo Brocchi","doi":"10.1128/genomeA.00488-18","DOIUrl":"https://doi.org/10.1128/genomeA.00488-18","url":null,"abstract":"<p><p>Draft genomes of three <i>Salmonella enterica</i> 4,[5],12:i:- (STi) strains isolated from human infections were obtained using Illumina sequencing. They were negative for the <i>fljBA</i> operon but positive for <i>hin</i>, and k-mer analyses revealed their identity as <i>S. enterica</i> 4,[5],12:i:- 08-1736 and <i>S</i> Typhimurium. A draft <i>S</i> Typhimurium sequence is described for comparison.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00488-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00507-18
Chenyu Zhang, Hu Shan, Jianxin Wen
Porcine reproductive and respiratory syndrome virus (PRRSV), which leads to tremendous economic losses worldwide, is currently one of the most threatening viruses for the swine industry. However, PRRSV outbreaks in West China are rarely reported, even though the virus has remained active for a long time across the country. In this study, we report an outbreak of the highly pathogenic PRRSV strain QTX, isolated from a pig farm located in Ningxia, a province in West China.
{"title":"Genomic Analysis of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Circulating in Pig Farms in West China.","authors":"Chenyu Zhang, Hu Shan, Jianxin Wen","doi":"10.1128/genomeA.00507-18","DOIUrl":"https://doi.org/10.1128/genomeA.00507-18","url":null,"abstract":"<p><p>Porcine reproductive and respiratory syndrome virus (PRRSV), which leads to tremendous economic losses worldwide, is currently one of the most threatening viruses for the swine industry. However, PRRSV outbreaks in West China are rarely reported, even though the virus has remained active for a long time across the country. In this study, we report an outbreak of the highly pathogenic PRRSV strain QTX, isolated from a pig farm located in Ningxia, a province in West China.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00507-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00637-18
Nicolas E Gaultier, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Carmon Kee, Balakrishnan N V Premkrishnan, Cassie E Heinle, Serene B Y Lim, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster
Serratia marcescens strain SGAir0764 was isolated from a tropical air sample collected in Singapore. The complete genome, sequenced on the PacBio RS II platform, consists of one chromosome with 5.1 Mb and one plasmid with 76.4 kb. Genome annotation predicts 4,723 protein-coding genes, 89 tRNAs, and 22 rRNAs.
{"title":"Complete Genome Sequence of the Bacterium Serratia marcescens SGAir0764, Isolated from Singapore Air.","authors":"Nicolas E Gaultier, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Carmon Kee, Balakrishnan N V Premkrishnan, Cassie E Heinle, Serene B Y Lim, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster","doi":"10.1128/genomeA.00637-18","DOIUrl":"https://doi.org/10.1128/genomeA.00637-18","url":null,"abstract":"<p><p><i>Serratia marcescens</i> strain SGAir0764 was isolated from a tropical air sample collected in Singapore. The complete genome, sequenced on the PacBio RS II platform, consists of one chromosome with 5.1 Mb and one plasmid with 76.4 kb. Genome annotation predicts 4,723 protein-coding genes, 89 tRNAs, and 22 rRNAs.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00637-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00576-18
S V Andryuschenko, I A Zdvizhkova, N B Perunova, O V Bukharin
This report describes the draft genome sequence of Klebsiella pneumoniae strain ICIS-278_PBV, isolated from the feces of a healthy 59-year-old man from Orenburg, Russia. The size of the genome was 5,584,615 bp (57.2% G+C content). Annotation revealed 5,302 coding sequences, including 5,254 proteins, 23 rRNA genes, and 81 tRNA genes.
本报告描述了从俄罗斯奥伦堡一名59岁健康男子粪便中分离出的肺炎克雷伯菌菌株ICIS-278_PBV的基因组序列草图。基因组大小为5,584,615 bp (G+C含量为57.2%)。注释共发现5302个编码序列，其中蛋白质5254个，rRNA基因23个，tRNA基因81个。
{"title":"Draft Genome Sequence of Klebsiella pneumoniae Strain ICIS-278_PBV, Isolated from the Feces of a Healthy 59-Year-Old Man from Orenburg, Russia.","authors":"S V Andryuschenko, I A Zdvizhkova, N B Perunova, O V Bukharin","doi":"10.1128/genomeA.00576-18","DOIUrl":"https://doi.org/10.1128/genomeA.00576-18","url":null,"abstract":"<p><p>This report describes the draft genome sequence of <i>Klebsiella pneumoniae</i> strain ICIS-278_PBV, isolated from the feces of a healthy 59-year-old man from Orenburg, Russia. The size of the genome was 5,584,615 bp (57.2% G+C content). Annotation revealed 5,302 coding sequences, including 5,254 proteins, 23 rRNA genes, and 81 tRNA genes.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00576-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.1128/genomeA.00642-18
Serene B Y Lim, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Nicolas E Gaultier, Balakrishnan N V Premkrishnan, Cassie E Heinle, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster
Bacillus velezensis strain SGAir0473 (Firmicutes) was isolated from tropical air collected in Singapore. Its genome was assembled using short reads and single-molecule real-time sequencing and comprises one chromosome with 4.18 Mb. The genome consists of 3,937 protein-coding genes, 86 tRNAs, and 27 rRNAs.
{"title":"Genome Sequence of Bacillus velezensis SGAir0473, Isolated from Tropical Air Collected in Singapore.","authors":"Serene B Y Lim, Ana Carolina M Junqueira, Akira Uchida, Rikky W Purbojati, James N I Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Nicolas E Gaultier, Balakrishnan N V Premkrishnan, Cassie E Heinle, Vineeth Kodengil Vettath, Daniela I Drautz-Moses, Stephan C Schuster","doi":"10.1128/genomeA.00642-18","DOIUrl":"https://doi.org/10.1128/genomeA.00642-18","url":null,"abstract":"<p><p><i>Bacillus velezensis</i> strain SGAir0473 (<i>Firmicutes</i>) was isolated from tropical air collected in Singapore. Its genome was assembled using short reads and single-molecule real-time sequencing and comprises one chromosome with 4.18 Mb. The genome consists of 3,937 protein-coding genes, 86 tRNAs, and 27 rRNAs.</p>","PeriodicalId":12619,"journal":{"name":"Genome Announcements","volume":"6 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/genomeA.00642-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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