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Comparison of Fungal and Bacterial Microbiomes of Bats and Their Cave Roosting Environments at El Malpais National Monument, New Mexico, USA 美国新墨西哥州El Malpais国家纪念地蝙蝠真菌和细菌微生物群及其洞穴栖息环境的比较
IF 2.3 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-11-27 DOI: 10.1080/01490451.2023.2283427
Jennifer J. M. Hathaway, Paris S. Salazar-Hamm, Nicole A. Caimi, Donald O. Natvig, Debbie C. Buecher, Diana E. Northup
The arrival in North America of white-nose syndrome (WNS), a devastating fungal disease in bats, has emphasized the necessity of a comprehensive understanding of the bats’ external skin microbiota....
白鼻综合征(WNS)是蝙蝠中一种毁灭性的真菌疾病,它在北美的出现强调了全面了解蝙蝠外部皮肤微生物群....的必要性
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引用次数: 0
Effects of Feldspathic Sandstone Addition on Microbial Biomass, Soil Enzyme Activity, and Bacterial Community Structure of Sandy Soil 添加长石砂岩对沙质土壤微生物生物量、土壤酶活性和细菌群落结构的影响
IF 2.3 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-11-16 DOI: 10.1080/01490451.2023.2272619
Jichang Han, Yang Zhang, Ting Wei
Desertification is a serious environmental problem worldwide. In this study, a long-term field experiment was conducted to investigate the effects of feldspathic sandstone on remediation of sandy s...
荒漠化是一个全球性的严重环境问题。本文通过长期的野外试验,研究了长石砂岩对沙质土壤的修复作用。
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引用次数: 0
Photocatalytic Removal of Anthracene using Zinc Oxide Nanoparticles Synthesized by Fusarium proliferatum WC416 增殖镰刀菌WC416合成氧化锌纳米颗粒光催化脱除蒽
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-11-01 DOI: 10.1080/01490451.2023.2272621
Shalini Gupta, Raman Kumar Ravi, Bhawana Pathak
AbstractRelease of complex waste in environment due to massive industrialization is a major threat globally. Anthracene is a polycyclic aromatic hydrocarbon generally released as a waste from petrochemical industries. It may bioaccumulate in food chain which results into nephrotoxicity in humans. Thus, there is a necessity to clean up the contaminated site efficiently. Bioremediation is ecofriendly technique for waste reclamation however it consumes more time. Thus, nanotechnology has exploded with unique properties of nanomaterials. Bio-based nanoparticles (NPs) synthesis is a recent and agro ecologically friendly approach for remediating environmental waste. For the first time present study reports Fusarium proliferatum WC416 isolated from refinery effluent for the synthesis of zinc oxide nanoparticles (ZnO NPs). Moreover, biosynthesized ZnO NPs used for their photocatalytic efficiency to remove anthracene at varying concentration. During synthesis of ZnO in aqueous medium showed a characteristic peak at 365 nm as analyzed by Ultraviolet–visible spectrum. The formation of NPs was further confirmed by Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and energy-dispersive x-ray (XRD). Morphologically the synthesized ZnO NPs were found to be in irregular polygonal shapes. Moreover, ZnO NPs showed photocatalytic activity for removal of anthracene upto 65 ± 0.03%, p value = 0.00032 at 100 ppm followed by 50.7% ± 0.02%, p value = 0.00023 at 250 ppm and 40.83 ± 0.03%, p value = 0.00042 at 500 ppm within 6 hr duration. Anthracene transformation during photocatlytic activity was confirmed by GC MS analysis, and the products were detected as 1,2 benzene carboxylic acid (m/z 166) and salicylic acid (m/z 138). Phytotoxicity effect of synthesized ZnO NPs on seed germination index of Phaseolus moonga and Vigina radiata was also studied to promote sustainable and biocompatible nano-agriculture.Keywords: AnthraceneF. proliferatum WC416fungal synthesiszinc oxide nanoparticles (ZnO NPs) AcknowledgementThe author is thankful to School of Environment and Sustainable development, Central University of Gujarat for providing research facilities.Author contributionsAll authors contributed to the research conception and design. Material preparation, data collection, analysis and results interpretation were performed by (Shalini Gupta), (Bhawana Pathak) and (Raman Kumar Ravi). The manuscript was written by (Shalini Gupta), correction and edited by (Raman Kumar Ravi) and finalization of manuscript was done by (Bhawana Pathak). All authors read and approved the final manuscript.Disclosure statementNo potential conflict of interest was reported by the authors.The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.Data availability statementAll data generated or analyzed during this study are included in this manuscript.
大规模工业化导致的复杂废弃物排放是全球面临的一大威胁。蒽是一种多环芳烃,通常作为石油化工废弃物排放。它可能在食物链中生物积累,导致人体肾毒性。因此,有必要对污染场地进行有效的清理。生物修复技术是一种环保的垃圾回收技术,但耗时较长。因此,纳米技术以其独特的纳米材料特性而爆发。生物基纳米颗粒(NPs)的合成是一种最新的农业生态友好的方法,用于修复环境废物。本研究首次报道了从炼油厂废水中分离得到的增殖镰刀菌WC416用于合成氧化锌纳米颗粒(ZnO NPs)。此外,生物合成的ZnO NPs在不同浓度下具有光催化脱除蒽的效率。通过紫外可见光谱分析,ZnO在水溶液中合成时在365 nm处有一个特征峰。傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)和能量色散x射线(XRD)进一步证实了NPs的形成。形貌上,合成的ZnO纳米粒子呈不规则的多边形形状。此外,ZnO NPs在6小时内对蒽的光催化活性最高可达65±0.03%,在100 ppm时p值= 0.00032,其次为50.7%±0.02%,在250 ppm时p值= 0.00023,在500 ppm时p值= 40.83±0.03%,p值= 0.00042。GC - MS分析证实了光催化过程中蒽的转化,产物为1,2苯羧酸(m/z 166)和水杨酸(m/z 138)。为了促进纳米农业的可持续发展和生物相容性,还研究了合成氧化锌NPs对菜豆和红枣种子萌发指数的植物毒性影响。关键词:AnthraceneF。作者感谢印度古吉拉特邦中央大学环境与可持续发展学院提供的研究设施。所有作者都对研究的构思和设计做出了贡献。材料准备、数据收集、分析和结果解释由Shalini Gupta、Bhawana Pathak和Raman Kumar Ravi完成。手稿由沙利尼·古普塔(Shalini Gupta)撰写,由拉曼·库马尔·拉维(Raman Kumar Ravi)修改和编辑,手稿的定稿由巴瓦纳·帕塔克(Bhawana Pathak)完成。所有作者都阅读并批准了最终的手稿。披露声明作者未报告潜在的利益冲突。作者声明在撰写本文期间未收到任何资金、资助或其他支持。数据可用性声明本研究过程中产生或分析的所有数据都包含在本文中。
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引用次数: 0
Phosphate Solubilizing Microorganisms Increase Soil Phosphorus Availability: A Review 增磷微生物提高土壤磷有效性的研究进展
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-10-24 DOI: 10.1080/01490451.2023.2272620
Feng Liu, Jin Qian, Yueming Zhu, Peifang Wang, Jing Hu, Bianhe Lu, Yuxuan He, Sijing Tang, Junwei Shen, Yin Liu, Fengduo Li
AbstractPhosphorus (P) availability in soil is often limited, posing a challenge for sustainable agricultural production. Phosphate solubilizing microorganisms (PSMs) have emerged as a promising biological approach to enhance soil P availability while minimize negative environmental impacts. Exploring the impact of PSMs on increasing soil available P is crucial for optimizing the utilization of insoluble P in soil and promoting sustainable agricultural practices. In this article, we provide a comprehensive review on the dynamics of soil available P, including its input, loss and transformation processes. Additionally, we draw a conclusion about how PSMs increase the availability of P in soil, including solubilizing inorganic P (Pi) and organic P (Po) by secreting substances such as protons, organic acids, inorganic acids, exopolysaccharides, hydrogen sulfide, siderophores and phosphatases. Notably, the factors influencing the P solubilizing activity of PSMs in soil are thoroughly discussed. Moreover, practical applications of PSMs for increasing soil P availability are summarized. Future research should focus on exploring novel PSM isolates, conducting long-term field experiments to assess the effects and potential risk of PSMs under diverse conditions, and investigating synergistic approaches with other P activators or beneficial microorganisms. This review will advance our understanding of PSMs and their practical application, enabling their widespread adoption in agricultural systems.Keywords: Potential riskpractical applicationsustainable agricultural production Author contributionsFeng Liu: Writing-original draft preparation. Jin Qian: Supervision. Yueming Zhu, Peifang Wang and Jing Hu: Reviewing and editing. Bianhe Lu, Yuxuan He, and Sijing Tang: Data Curation. Junwei Shen, Yin Liu and Fengduo Li: Visualization.Disclosure statementThe authors declare no potential conflict of interest.Additional informationFundingThis study was supported by the National Natural Science Foundation of China [No. 51779078], the Six Talent Peaks Project in Jiangsu Province [No. JNHB-012], the National Major Projects of Water Pollution Control and Management Technology [No. 2017ZX07204003], and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
摘要土壤中磷的有效性往往是有限的,这对农业可持续生产提出了挑战。磷酸盐增溶微生物(psm)已成为一种有前途的生物方法,以提高土壤磷有效性,同时尽量减少对环境的负面影响。探索土壤磷素对提高土壤速效磷的影响,对于优化土壤不溶性磷的利用和促进可持续农业实践具有重要意义。本文对土壤有效磷的输入、流失和转化过程进行了综述。此外,我们还得出了psm如何通过分泌质子、有机酸、无机酸、外多糖、硫化氢、铁载体和磷酸酶等物质来溶解无机磷(Pi)和有机磷(Po)的结论。特别讨论了影响psm在土壤中溶磷活性的因素。此外,还总结了psm在提高土壤磷素有效性方面的实际应用。未来的研究应侧重于探索新的PSM分离物,进行长期的现场实验以评估PSM在不同条件下的效果和潜在风险,并研究与其他P激活剂或有益微生物的协同方法。这一综述将促进我们对psm及其实际应用的理解,使其在农业系统中得到广泛采用。关键词:潜在风险实际应用可持续农业生产作者贡献刘峰:写作-原稿准备金谦:监管。朱跃明、王培芳、胡静:述评与编辑。陆汴河,何宇轩,唐思静:数据策展。沈俊伟,刘寅,李凤铎:可视化。作者声明没有潜在的利益冲突。本研究由国家自然科学基金资助[No. 5];51779078],江苏省六大人才高峰工程项目[No. 51779078];国家水污染控制与治理技术重大专项[JNHB-012];2017ZX07204003],江苏省高等学校重点学科建设项目(PAPD)。
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引用次数: 0
The Effect of Heavy Metals on Bacterial Community Structure and Functional Profile in Surface Soils of Baiji Oil Refinery and Baghdad City, Iraq 重金属对伊拉克白集炼油厂和巴格达市表层土壤细菌群落结构和功能的影响
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-10-18 DOI: 10.1080/01490451.2023.2270482
Ahmed Ismael Naqee Al-Bayati, Hülya Saide Özkoç, Luay Burhan Mustafa, Ibrahim Özkoç
AbstractThe increasing accumulation of heavy metals (HMs) due to various human activities is a significant concern. This study has employed 16S rRNA amplicon sequencing and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) spectrometry to investigate HMS concentrations and identify bacterial strains exhibiting resistance to HMS, and used the PICRUSt tool to assess the metabolic pathways of bacterial communities. Our primary focus was to assess HMs concentrations in the soils of Baghdad (BG) and the Baiji Oil Refinery (BOR) area while examining the bacterial diversity in these regions. The results revealed that the average levels of Cadmium (Cd) and Lead (Pb) in BOR were higher than in BG and the global average. In BOR, the most prevalent bacterial genera were Flavobacterium, Shewanella, and Pseudomonas. Using the PICRUSt tool, we identified that Cd and Pb substantially impacted the metabolic pathways of these bacterial communities. This study marks the first report in Iraq to employ 16S rRNA gene sequencing to analyze bacterial communities in Iraqi soil and investigate how HMs affect them. However, this study can serve as a reference for future research and the development of innovative strategies for the sustainable management of soil ecosystems.Keywords: Baiji oil refineryenvironmental assessmentheavy metal contaminationmetabolic pathwaysmetagenomics Disclosure statementNo potential conflict of interest was reported by the author(s).
摘要各种人类活动引起的重金属积累日益严重,已引起人们的广泛关注。本研究采用16S rRNA扩增子测序和电感耦合等离子体质谱法(ICP-MS)测定HMS浓度,鉴定对HMS耐药的菌株,并使用PICRUSt工具评估细菌群落的代谢途径。我们的主要重点是评估巴格达(BG)和Baiji炼油厂(BOR)地区土壤中的HMs浓度,同时检查这些地区的细菌多样性。结果表明,BOR中镉(Cd)和铅(Pb)的平均含量高于BG和全球平均水平。在BOR中,最常见的细菌属是黄杆菌、希瓦氏菌和假单胞菌。使用PICRUSt工具,我们发现Cd和Pb显著影响了这些细菌群落的代谢途径。这项研究标志着伊拉克首次使用16S rRNA基因测序来分析伊拉克土壤中的细菌群落,并调查HMs如何影响它们。然而,本研究可为未来土壤生态系统可持续管理的研究和创新策略的制定提供参考。关键词:白集炼油厂环境评价重金属污染代谢途径宏基因组披露声明作者未发现潜在利益冲突。
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引用次数: 0
Isolation and Characterization of Rhizospheric Soil Bacteria Present in Shivrajpur Mn Mine Site Shivrajpur锰矿根际土壤细菌的分离与鉴定
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-10-13 DOI: 10.1080/01490451.2023.2259391
Tamanna Mehta, Shahenaz Jadeja, Tarika Kumar
AbstractDue to the obvious potential hazards it poses to the environment, animals, and human health, Mn (II) contamination has become worrisome. The broad aim of the study was to examine the contamination levels of soil in the Shivrajpur Mn mine site, physicochemical tests such as pH and heavy metal analyses were done. Later 16S rRNA sequencing was used to characterize bacterial species isolated from different colonizer plant samples. The bacterial strains recovered from Mn mine rhizospheric soil exemplified morphological and molecular diversity. It was discovered that all seven bacterial isolates could survive Mn salt concentrations up to 2000 mg/l, whereas only one, Staphylococcus capitis (GenBank OP861008), could withstand concentrations up to 10,000 mg/l. The three isolates, Staphylococcus capitis, Bacillus albus (GenBank OP861099), Bacillus tequilensis (GenBank OQ135205), have the ability to sustain the Mn tolerance as the agar wells were surrounded by growth expanse. The findings imply that the Staphylococcus capitis could be used to create a biosorbent that is both effective and environmentally benign, and it can aid in the bioremediation of contaminated soil.Keywords: BacteriabioremediationMn contaminationmolecular diversity16SrRNA sequencing AcknowledgementsWe are grateful to Prof. Kauresh Vachhrajani, Former Head and Professor of Zoology, The Maharaja Sayajirao University of Baroda for his vision and guiding force for taking up this initiative. We also express our gratitude to Prof. P. Padmaja Sudhakar, Officiating Head, Department of Environmental Studies and Prof. H.R. Kataria, Dean, Faculty of Science, The Maharaja Sayajirao University of Baroda for constant support and encouragement.Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by Research and Consultancy Cell, The Maharaja Sayajirao University of Baroda.
摘要锰(II)污染对环境、动物和人体健康具有明显的潜在危害,令人担忧。该研究的主要目的是检查Shivrajpur锰矿场土壤的污染水平,进行了物理化学测试,如pH值和重金属分析。随后使用16S rRNA测序来鉴定从不同定植植物样品中分离的细菌种类。从锰矿根际土壤中恢复的菌株表现出形态和分子的多样性。结果发现,所有7株细菌都能在锰盐浓度高达2000 mg/l的情况下存活,而只有一株猪头葡萄球菌(Staphylococcus capitis, GenBank OP861008)能承受高达10,000 mg/l的浓度。头葡萄球菌、白色芽孢杆菌(GenBank OP861099)、龙舌兰芽孢杆菌(GenBank OQ135205)这3株菌株由于琼脂孔被生长空间包围,具有耐锰能力。这一发现表明,头葡萄球菌可以用来制造一种既有效又对环境无害的生物吸附剂,它可以帮助污染土壤的生物修复。感谢巴罗达Maharaja Sayajirao大学前动物学主任和教授Kauresh Vachhrajani教授的远见卓识和开展这项工作的指导力量。同时,我们也要感谢巴罗达马哈王纪酒庄大学环境研究系主任P. Padmaja Sudhakar教授和理学院院长H.R. Kataria教授一直以来的支持和鼓励。披露声明作者未报告潜在的利益冲突。本研究由巴罗达玛哈茹阿贾·Sayajirao大学研究与咨询小组支持。
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引用次数: 0
Application of Microorganisms in Biotransformation and Bioremediation of Environmental Contaminant: A Review 微生物在环境污染物生物转化与生物修复中的应用综述
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-10-06 DOI: 10.1080/01490451.2023.2261443
Moupriya Nag, Dibyajit Lahiri, Sreejita Ghosh, Tanmay Sarkar, Siddhartha Pati, Alok Prasad Das, Deo Karan Ram, Debasmita Bhattacharya, Rina Rani Ray
AbstractThe increase in population has resulted in a rapid increase in waste that is being dumped within the environment. The addition of various harmful and contaminating compounds results in the degradation of the environment and has a direct impact on human health. Various research studies that are taking place in recent times help in the enhancement of the naturally available microbes to degrade toxic compounds that are liberated into the environment. Although various types of bioremediation techniques are available, microbe-associated remediation is proven to be the best option due to its effectiveness, no generation of toxic by-products, and natural availability of microbes with the ability to take up nutrients from the toxic contaminants that exist on the surface of the earth. Both ex situ and in situ bioremediation can be accomplished with the help of these microbes. In most cases, this is done through biofilm-assisted seclusion of pollutants, more precisely by means of extracellular polymeric substances (EPSs) of biofilm matrix. In recent times, various types of techniques have been implemented like proteomics, genomics, fluxomics and transcriptomics for bringing about maximum remediation by the microbial species. In order to explore the different kinds of microbial communities involved in natural bioremediation, the strategic approach of metagenomic analysis is adopted, which has brought about a technological drift in remediation, even at a genomic level. As chemical mode of remediating wastes are associated with the release of various harmful chemicals those can be toxic to the environment, the use of biological systems can be a potential agent in the conversion of complex waste materials to simpler substances so that it mixes with the environment in easier manner. Microbes are the potential entities that can easily act on the waste materials and bring about remediation in an effective manner.Keywords: Biofilmbioremediationmicrobesomicsorganic wastepollutant Disclosure statementNo potential conflict of interest was reported by the author(s).
【摘要】人口的增长导致了向环境中倾倒垃圾的迅速增加。各种有害和污染化合物的加入导致环境退化,并对人类健康产生直接影响。最近正在进行的各种研究有助于增强自然可用的微生物来降解释放到环境中的有毒化合物。尽管有各种类型的生物修复技术可供使用,但微生物相关的修复被证明是最佳选择,因为它有效,不会产生有毒副产物,并且微生物具有从地球表面存在的有毒污染物中吸收营养物质的能力。在这些微生物的帮助下,可以完成非原位和原位生物修复。在大多数情况下,这是通过生物膜辅助的污染物隔离来实现的,更准确地说,是通过生物膜基质的细胞外聚合物物质(eps)来实现的。近年来,蛋白质组学、基因组学、通量组学和转录组学等多种技术被广泛应用,以最大限度地发挥微生物物种的修复作用。为了探索参与自然生物修复的不同种类的微生物群落,采用了宏基因组分析的战略方法,这带来了修复的技术漂移,甚至在基因组水平上。由于废物的化学修复方式与各种有害化学物质的释放有关,这些化学物质可能对环境有毒,因此使用生物系统可以将复杂的废物转化为更简单的物质,使其更容易与环境混合。微生物是一种很容易作用于废弃物并能有效修复的潜在实体。关键词:生物膜生物修复微生物组有机废物污染物披露声明作者未报告潜在利益冲突。
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引用次数: 1
Bacterial Communities Associated with Tufa Deposits and Characterization of Indigenous Calcifying Bacterial Isolates from Jiuzhaigou National Nature Reserve, Sichuan, China 九寨沟自然保护区凝灰岩沉积细菌群落特征及原生钙化细菌分离株
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-09-29 DOI: 10.1080/01490451.2023.2261437
Shida Li, Kaixin Liu, Jie Du, Cong Chen, Ningfei Lei, Xiangjun Pei, Jin Tong, Weiyang Xiao, Hongguan Xie
AbstractTufa is a common calcium carbonate deposit in karst landscapes and microbes may play an important role in its formation. As an important natural resource treasure trove, the diversity of bacteria from the Jiuzhaigou National Nature Reserve tufa deposits and their potential to deposit carbonates are still unknown. In this study, 16S rRNA gene sequencing technology was used to assess the bacterial community composition of Pearl Shoal in Jiuzhaigou National Nature Reserve. The results showed that the main dominant phyla were Proteobacteria, Cyanobacteria, Bacteroidetes and Actinobacteria. With the gradual increase of flow rate, the bacterial community structure was gradually simplified and the dominant phylum changed. The community structure of different sampling sites was significantly different. Besides, 17 strains capable of precipitating calcium carbonate were isolated using microbiological methods. The two strains with relatively strong calcium-forming properties were identified as Leucobacter sp. (J1) and Brucella sp. (J2), respectively. Both strains were able to elevate the environmental pH and produce extracellular polymeric substance (EPS). However, Leucobacter sp. (J1) formed calcite crystals with spherical structure, whereas Brucella sp. (J2) formed the rhombohedral and some spherical calcite crystals, evidence for strain-specific characteristics. It is the first research to provide information regarding the diversity of bacterial communities associated with tufa deposits and the characterization of calcifying bacteria isolated from Jiuzhaigou National Nature Reserve.Keywords: Calcifying strainshigh-throughput sequencingJiuzhaigou National Nature ReserveTufa Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by the Specialized Fund for Ecological Restoration of Jiuzhaigou [N51322112022000246].
摘要凝灰岩是岩溶景观中常见的碳酸钙沉积,微生物可能在其形成过程中起重要作用。作为重要的自然资源宝库,九寨沟国家级自然保护区凝灰岩矿床的细菌多样性及其沉积碳酸盐岩的潜力尚不清楚。本研究采用16S rRNA基因测序技术对九寨沟国家级自然保护区珍珠滩细菌群落组成进行了研究。结果表明,主要优势门为变形菌门、蓝藻门、拟杆菌门和放线菌门。随着流量的逐渐增大,细菌群落结构逐渐简化,优势门发生变化。不同样点的群落结构差异显著。此外,采用微生物学方法分离得到17株能沉淀碳酸钙的菌株。两株产钙能力较强的菌株分别为Leucobacter sp. (J1)和Brucella sp. (J2)。两株菌株均能提高环境pH值并产生胞外聚合物(EPS)。然而,白杆菌sp. (J1)形成球形结构的方解石晶体,而布鲁氏菌sp. (J2)形成菱形和一些球形方解石晶体,证明了菌株特有的特征。这是对九寨沟自然保护区凝灰岩沉积物细菌群落多样性和钙化细菌特征的首次研究。关键词:钙化菌株;高通量测序;九寨沟国家级自然保护区;项目资助:九寨沟生态修复专项基金[N51322112022000246]。
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引用次数: 0
Review on the Application of Biogeotechnology in the Restoration of Masonry Structures 生物岩土技术在砌体结构修复中的应用综述
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-09-25 DOI: 10.1080/01490451.2023.2255595
Guangning Xiao, Jiangwei Zhang, Xiaohui Cheng
AbstractBiogeotechnology is an interdisciplinary technology to serves engineering by controlling the mineralization process of microorganisms. It has become one of the hot research topics in geotechnical engineering and related disciplines in recent years. Based on the application achievements of biogeotechnology in the restoration of masonry structures, this paper reviews the microbial mineralization reaction mechanisms and influencing factors, and summarizes the research results from the two construction techniques of surface bio-deposited layer and sealing reinforcement of masonry. The results show that biogeotechnology is feasible in the restoration and protection of masonry structures, and can effectively improve the physical and mechanical properties of restorations. It has the advantages of environmental friendliness and controllable reactions. However, large-scale applications in this field are relatively rare, which need to be further discussed and investigated in combination with indoor and outdoor experiments.Keywords: BiogeotechnologybiomineralizationMICPmasonry structure Disclosure statementNo potential conflict of interest was reported by the author(s).AcknowledgementThe authors express their sincere thanks to the National Natural Science Foundation of China (52274149) for the support.
摘要生物岩土技术是一门通过控制微生物矿化过程服务于工程的交叉学科技术。近年来已成为岩土工程及相关学科的研究热点之一。基于生物土工技术在砌体结构修复中的应用成果,综述了微生物矿化反应机理及影响因素,总结了表面生物堆积层和砌体密封加固两种施工技术的研究成果。结果表明,生物土工技术在砌体结构修复与保护中是可行的,可以有效地改善修复体的物理力学性能。具有环境友好、反应可控等优点。然而,该领域的大规模应用相对较少,需要进一步结合室内和室外实验进行讨论和研究。关键词:生物地质技术生物矿化micpstone结构披露声明作者未报告潜在利益冲突。感谢国家自然科学基金(52274149)对作者的支持。
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引用次数: 0
Accelerated Degradation of four Organophosphorus Insecticides by Malathion Tolerant Aspergillus niger MRU01 a Soil Fungus 耐马拉硫磷黑曲霉MRU01加速降解4种有机磷杀虫剂的研究
4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Pub Date : 2023-09-21 DOI: 10.1080/01490451.2023.2259912
Debasish Mohapatra, Sakti Kanta Rath, Pradipta K. Mohapatra
AbstractInsecticides are widely used in agriculture, horticulture, medicine, and industry, as well as domestically. Increased insecticide application has been attributed to higher agricultural productivity and lower infield and postharvest crop loss in the twentieth century. Malathion is a very commonly used organophosphorus (OP) insecticide applied in over 100 food crops in agriculture and post-harvest storage. Different species of Aspergillus have shown their efficiency to degrade OP chemicals. A malathion tolerant Aspergillus niger MRU01, developed by prolonged malathion exposure, was tested for its efficiency to remove malathion as well as three other commonly used OP insecticides, viz., parathion, chlorpyrifos and dimethoate. The tolerant strain showed growth efficiency as well as enhanced production of phosphatases and esterase enzyme as compared to untreated and treated wild type (A. niger ATCC16404). The removal of malathion was 29% and 68%, in the wild type and the tolerant strain, respectively during five days. The tolerant strain was also able to remove dimethoate (63%) and chlorpyrifos (54%) with high efficiency while a low rate of removal of parathion (33%) was observed. The strain can be tested in the microcosms more specifically for bioremediation of other OP insecticides before its application in the field.HIGHLIGHTSThe malathion tolerant Aspergillus niger MRU01 grew efficiently in the presence of 500 μM malathion.Treatment of the fungus with malathion and other OP insecticides – parathion, chlorpyrifos, and dimethoate – caused enhanced expression of phosphatases and esterases.The tolerant A. niger MRU01 strain had significantly lower mycelia concentrations of insecticides than of the wild type A. niger ATCC16404.Degradation of insecticides by the tolerant strain was significantly higher than by the wild type indicating its broad spectrum of tolerance.Keywords: Aspergillus nigermalathion mutantOP insecticidesesterasesphosphatasesremoval AcknowledgmentsThe research was partly funded by Department of Science and Technology, Government of Odisha, and OHEPEE, Government of Odisha. The infrastructure grants of DST, Government of India under DST-FIST programme is gratefully acknowledged.Authors’ contributionsAll authors contributed to the study conception and design. Conceptualization, investigation, original draft was performed by Debasish Mohapatra. Data curation, formal analysis was done by Sakti Kanta Rath and project administration, data analysis, review and editing was performed by Pradipta K Mohapatra. All authors read and approved the final manuscript.”Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by Department of Science and Technology, Government of Odisha, and OHEPEE, Government of Odisha. The infrastructure grants of DST, Government of India under DST-FIST
摘要农药广泛应用于农业、园艺、医药、工业和家庭。杀虫剂使用量的增加归因于20世纪农业生产力的提高和田间和收获后作物损失的减少。马拉硫磷是一种非常常用的有机磷杀虫剂,应用于农业和收获后储存的100多种粮食作物中。不同种类的曲霉已经显示出它们对OP化学物质的降解效率。通过长期接触马拉硫磷而培育出的耐马拉硫磷黑曲霉MRU01,对其去除马拉硫磷以及其他三种常用的有机磷杀虫剂(对硫磷、毒死蜱和乐果)的效率进行了测试。与未经处理和处理的野生型(A. niger ATCC16404)相比,该耐药菌株表现出生长效率,并提高了磷酸酶和酯酶的产量。野生型和耐受性菌株在5 d内对马拉硫磷的去除率分别为29%和68%。耐药菌株对乐果(63%)和毒死蜱(54%)的去除率较高,对硫磷(33%)的去除率较低。该菌株在野外应用前可在微生物中进行更特异性的生物修复试验,以便对其他有机磷杀虫剂进行生物修复。耐马拉硫磷黑曲霉MRU01在500 μM马拉硫磷环境下生长良好。用马拉硫磷和其他有机磷杀虫剂(对硫磷、毒死蜱和乐果)处理真菌可增强其磷酸酶和酯酶的表达。耐药菌株MRU01菌丝体杀虫剂浓度显著低于野生型菌株ATCC16404。耐受性菌株对杀虫剂的降解率明显高于野生型,表明其耐受性谱广。本研究由奥里萨邦政府科技部和奥里萨邦政府OHEPEE部分资助。感谢印度政府DST在DST- first方案下提供的基础设施赠款。作者的贡献所有作者都对研究的构思和设计做出了贡献。概念化,调查,原稿由Debasish Mohapatra完成。数据管理、形式分析由Sakti Kanta Rath完成,项目管理、数据分析、审查和编辑由Pradipta K Mohapatra完成。所有作者都阅读并认可了最终的手稿。”披露声明作者未报告潜在的利益冲突。这项工作得到了奥里萨邦政府科技部和奥里萨邦政府OHEPEE的支持。印度政府在DST- first下的DST基础设施拨款
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引用次数: 1
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Geomicrobiology Journal
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