Pub Date : 2023-04-04DOI: 10.1080/01490451.2023.2192216
Meng Q Zhang, Liang Zhao, Gen K. Li, Chenliang Zhu, Dan Xu, Junfeng Ji, Jun Chen
Abstract CO2 mineral carbonation induced by microalgae is an emerging approach to carbon capture, utilization, and storage (CCUS). Freshwater cyanobacteria are common microalgae in nature that can raise the pH of eutrophic waters and drive the precipitation of carbonate. Still, limited studies have been conducted to evaluate and select appropriate cyanobacteria strains for CCUS. Here we present experimental investigations to compare the capacity of different freshwater cyanobacterial strains in converting CO2 to carbonates. We compare five cyanobacterial strains by monitoring their growth curves. We examine three metrics, the maximum pH of the solution, hydroxide production capacity, and extracellular polymeric substances (EPS) secretion capacity, to assess the capacity for carbon sequestration. Our results indicate that among the five strains, Microcystis aeruginosa shows the highest pH and EPS content per unit cell number, marking the most significant capacity for CO2 carbonation. We observe carbonate precipitates as hydromagnesite and dypingite. We suggest the negatively charged, hydrophilic EPS can effectively promote the precipitation of magnesium carbonate and inhibit the precipitation of calcium carbonate. Overall, our approach provides a framework for assessing and selecting cyanobacterial strains for CO2 carbonation, advancing the understanding of the mechanism of microalgae-induced CO2 Mg-carbonation from the perspective of EPS surface properties.
{"title":"Assessment and Selection of Cyanobacterial Strains for CO2 Mineral Sequestration: Implications for Carbonation Mechanism","authors":"Meng Q Zhang, Liang Zhao, Gen K. Li, Chenliang Zhu, Dan Xu, Junfeng Ji, Jun Chen","doi":"10.1080/01490451.2023.2192216","DOIUrl":"https://doi.org/10.1080/01490451.2023.2192216","url":null,"abstract":"Abstract CO2 mineral carbonation induced by microalgae is an emerging approach to carbon capture, utilization, and storage (CCUS). Freshwater cyanobacteria are common microalgae in nature that can raise the pH of eutrophic waters and drive the precipitation of carbonate. Still, limited studies have been conducted to evaluate and select appropriate cyanobacteria strains for CCUS. Here we present experimental investigations to compare the capacity of different freshwater cyanobacterial strains in converting CO2 to carbonates. We compare five cyanobacterial strains by monitoring their growth curves. We examine three metrics, the maximum pH of the solution, hydroxide production capacity, and extracellular polymeric substances (EPS) secretion capacity, to assess the capacity for carbon sequestration. Our results indicate that among the five strains, Microcystis aeruginosa shows the highest pH and EPS content per unit cell number, marking the most significant capacity for CO2 carbonation. We observe carbonate precipitates as hydromagnesite and dypingite. We suggest the negatively charged, hydrophilic EPS can effectively promote the precipitation of magnesium carbonate and inhibit the precipitation of calcium carbonate. Overall, our approach provides a framework for assessing and selecting cyanobacterial strains for CO2 carbonation, advancing the understanding of the mechanism of microalgae-induced CO2 Mg-carbonation from the perspective of EPS surface properties.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"446 - 461"},"PeriodicalIF":2.3,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48066824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.1080/01490451.2023.2192211
F. Soares, J. Trovão, I. Tiago, S. Cardoso, Francisco Gil, L. Catarino, A. Portugal
Abstract Phototrophic organisms, such as microalgae and cyanobacteria, are known to be major contributors to stone decay. The purpose of this study was to assess the dolomitic limestone colonization by phototrophic organisms, using single vs artificial multispecies, under laboratory conditions. To achieve this aim, dolomitic limestone blocks were inoculated with single phototrophic species previously collected from the Old Cathedral of Coimbra, for a period of three months. In parallel, limestone blocks were also inoculated with a mixture of the same isolated single species, in order to compare the colonization capacities of both conditions. Results were evaluated based on visual inspection, surface covered area, colorimetric and SEM analyses. Results showed that the phototrophic organisms were able to colonize the dolomitic limestone blocks in both conditions (single vs artificial multispecies), but biofilm development was more enhanced when single species, rather than multispecies, were used. The obtained results also allowed to observe the capacity for endolithic colonization and the formation of small cavities by some species.
{"title":"Phototrophic Colonization in Dolomitic Limestone: Comparison between Single vs Artificial Multispecies","authors":"F. Soares, J. Trovão, I. Tiago, S. Cardoso, Francisco Gil, L. Catarino, A. Portugal","doi":"10.1080/01490451.2023.2192211","DOIUrl":"https://doi.org/10.1080/01490451.2023.2192211","url":null,"abstract":"Abstract Phototrophic organisms, such as microalgae and cyanobacteria, are known to be major contributors to stone decay. The purpose of this study was to assess the dolomitic limestone colonization by phototrophic organisms, using single vs artificial multispecies, under laboratory conditions. To achieve this aim, dolomitic limestone blocks were inoculated with single phototrophic species previously collected from the Old Cathedral of Coimbra, for a period of three months. In parallel, limestone blocks were also inoculated with a mixture of the same isolated single species, in order to compare the colonization capacities of both conditions. Results were evaluated based on visual inspection, surface covered area, colorimetric and SEM analyses. Results showed that the phototrophic organisms were able to colonize the dolomitic limestone blocks in both conditions (single vs artificial multispecies), but biofilm development was more enhanced when single species, rather than multispecies, were used. The obtained results also allowed to observe the capacity for endolithic colonization and the formation of small cavities by some species.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"434 - 445"},"PeriodicalIF":2.3,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43670216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Although residual tetracycline, NH4 +-N, and P in aquaculture wastewater have been frequently detected, their simultaneous removal has received little attention. The simultaneous and efficient removal of tetracycline, NH4 +-N, COD, and P in aquaculture wastewater was achieved with a laboratory-scale moving bed biofilm reactor (MBBR) under different influent C/N ratios. The results suggested that tetracycline could be degraded efficiently without using an additional organic carbon source. Most of the tetracycline (>80%), NH4 +-N (>73%), TN (>68%), TP (>59%), and COD (>78%) were synchronously removed under the higher C/N ratios. The electron transport system activity (ETSA), dissolved organic matter (DOM), and extracellular polymeric substances (EPS) were evaluated for biofilm performance during aquaculture wastewater remediation. The results indicated that the protein (PN) and polysaccharide (PS) concentrations, and fluorescence intensities were significantly different because the influent C/N ratios changed the biofilm activity. The ETSA values were lowest when tetracycline was the sole carbon source, which suggested that tetracycline affected the nitrification activity of biofilms. Sphingobacteriia, Betaproteobacteria, and Alphaproteobacteria were identified as the dominant bacterial classes. This study provides a new strategy for the removal of tetracycline and nutrients from aquaculture wastewater.
{"title":"Characteristics of Tetracycline Degradation Coupled Simultaneous Nitrification-Denitrification and Phosphorus Removal in Aquaculture Wastewater","authors":"Sicheng Shao, Minghui Wang, Jinfeng Zhong, Xiangwei Wu","doi":"10.1080/01490451.2023.2182929","DOIUrl":"https://doi.org/10.1080/01490451.2023.2182929","url":null,"abstract":"Abstract Although residual tetracycline, NH4 +-N, and P in aquaculture wastewater have been frequently detected, their simultaneous removal has received little attention. The simultaneous and efficient removal of tetracycline, NH4 +-N, COD, and P in aquaculture wastewater was achieved with a laboratory-scale moving bed biofilm reactor (MBBR) under different influent C/N ratios. The results suggested that tetracycline could be degraded efficiently without using an additional organic carbon source. Most of the tetracycline (>80%), NH4 +-N (>73%), TN (>68%), TP (>59%), and COD (>78%) were synchronously removed under the higher C/N ratios. The electron transport system activity (ETSA), dissolved organic matter (DOM), and extracellular polymeric substances (EPS) were evaluated for biofilm performance during aquaculture wastewater remediation. The results indicated that the protein (PN) and polysaccharide (PS) concentrations, and fluorescence intensities were significantly different because the influent C/N ratios changed the biofilm activity. The ETSA values were lowest when tetracycline was the sole carbon source, which suggested that tetracycline affected the nitrification activity of biofilms. Sphingobacteriia, Betaproteobacteria, and Alphaproteobacteria were identified as the dominant bacterial classes. This study provides a new strategy for the removal of tetracycline and nutrients from aquaculture wastewater.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"399 - 412"},"PeriodicalIF":2.3,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49411621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-06DOI: 10.1080/01490451.2023.2184883
Sujata Dabolkar, I. Furtado, N. Kamat
Abstract Goa is situated in the northwestern part of the Western Dharwar Craton (WDC) which is Asia’s major metallogenic province. The iron ore of Goa is associated with greenstone and occurs as bands, reefs, and lenses of Banded Hematite Quartzite (BHQ) and Banded Magnetite Quartzite (BMQ) none of these are explored for geo-microbiological dimensions, such as detection of microbioform Gold. The presence of metalliferous WDC in Goa affords sampling of auriferous materials from mining area having hitherto BHQ at latitude 15°29′54″ N and longitude 74° 03′44″ E) and BMQ at latitude 15°24′12″ N and longitude 74° 09′31″ E were collected, surface sterilized and drilled with a sterile driller to get the endolithic material under sterile conditions and sent for metagenomic analysis using Oxford nanopore sequencing. Both the samples showed the presence of microorganisms with Archea, such as Haloferax, Nitrososphaera, and Eubacteria, i.e., Bacillus, Ralstonia, Cupriavidus, Burkholderia, and Acetobacter. The traditional model of Banded Iron Deposition assumes precipitation by the oxidation of hydrothermal Fe (II), either via abiotic oxidation or biotic oxidation through chemo lithotrophic Eubacteria, such as cyanobacteria which produce oxygen. This paper reports the diversity and the presumptive role of these microorganisms in the biogeochemical cycling of metals, such as Fe, Mn, and gold (Au).
{"title":"Pioneer Studies on Metagenomic Evaluation of Diversity of Microbial Community in Banded Iron Formation (BIF) from Iron Ore Mining Belt of Goa, India","authors":"Sujata Dabolkar, I. Furtado, N. Kamat","doi":"10.1080/01490451.2023.2184883","DOIUrl":"https://doi.org/10.1080/01490451.2023.2184883","url":null,"abstract":"Abstract Goa is situated in the northwestern part of the Western Dharwar Craton (WDC) which is Asia’s major metallogenic province. The iron ore of Goa is associated with greenstone and occurs as bands, reefs, and lenses of Banded Hematite Quartzite (BHQ) and Banded Magnetite Quartzite (BMQ) none of these are explored for geo-microbiological dimensions, such as detection of microbioform Gold. The presence of metalliferous WDC in Goa affords sampling of auriferous materials from mining area having hitherto BHQ at latitude 15°29′54″ N and longitude 74° 03′44″ E) and BMQ at latitude 15°24′12″ N and longitude 74° 09′31″ E were collected, surface sterilized and drilled with a sterile driller to get the endolithic material under sterile conditions and sent for metagenomic analysis using Oxford nanopore sequencing. Both the samples showed the presence of microorganisms with Archea, such as Haloferax, Nitrososphaera, and Eubacteria, i.e., Bacillus, Ralstonia, Cupriavidus, Burkholderia, and Acetobacter. The traditional model of Banded Iron Deposition assumes precipitation by the oxidation of hydrothermal Fe (II), either via abiotic oxidation or biotic oxidation through chemo lithotrophic Eubacteria, such as cyanobacteria which produce oxygen. This paper reports the diversity and the presumptive role of these microorganisms in the biogeochemical cycling of metals, such as Fe, Mn, and gold (Au).","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"427 - 433"},"PeriodicalIF":2.3,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48047614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-27DOI: 10.1080/01490451.2023.2177363
M. Hoppert, A. Reimer, K. Sauter, J. Reitner
Abstract In the present study, microbialites from a stream in the Ammergau Alps (Northern Calcareous Alps) were examined with regard to morphology, mineralogy, and colonizing microorganisms. The carbonate buildups form regular (mainly laminated) stromatolite structures, essentially due to the activity of the cyanobacterium Rivularia. The filaments of the cyanobacterium form exopolymer sheaths that prevent the precipitation of calcite from the carbonate-supersaturated stream water at cell surfaces. A pattern of non-calcified, parallel tubes surrounded by a calcified scaffold is built up. The calcification pattern may be explained by the inhibitory activity of cyanobacterial sheath exopolymers. In lower layers of the carbonate buildup, microbial diversity increases due to colonization by mainly heterotrophic bacteria. The hollow tubes hitherto exempt from calcification are gradually being filled. Calcification pattern is discussed in view of fossil Girvanella-type stromatolites.
{"title":"Modern Rivularia Freshwater Stromatolites as Models for Formation of Laminated Stromatolitic Crusts","authors":"M. Hoppert, A. Reimer, K. Sauter, J. Reitner","doi":"10.1080/01490451.2023.2177363","DOIUrl":"https://doi.org/10.1080/01490451.2023.2177363","url":null,"abstract":"Abstract In the present study, microbialites from a stream in the Ammergau Alps (Northern Calcareous Alps) were examined with regard to morphology, mineralogy, and colonizing microorganisms. The carbonate buildups form regular (mainly laminated) stromatolite structures, essentially due to the activity of the cyanobacterium Rivularia. The filaments of the cyanobacterium form exopolymer sheaths that prevent the precipitation of calcite from the carbonate-supersaturated stream water at cell surfaces. A pattern of non-calcified, parallel tubes surrounded by a calcified scaffold is built up. The calcification pattern may be explained by the inhibitory activity of cyanobacterial sheath exopolymers. In lower layers of the carbonate buildup, microbial diversity increases due to colonization by mainly heterotrophic bacteria. The hollow tubes hitherto exempt from calcification are gradually being filled. Calcification pattern is discussed in view of fossil Girvanella-type stromatolites.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"382 - 398"},"PeriodicalIF":2.3,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42837647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-23DOI: 10.1080/01490451.2023.2181469
R. Farkas, M. Toumi, Gorkhmaz Abbaszade, K. Bóka, K. Takáts, E. Tóth
Abstract In the present study, a 3-week microcosm experiment was conducted to assess the acute effect of arsenic trioxide [As(III)] at various concentrations (2.03, 4.06, 7.61, and 25.38 mM) on the bacterial communities. Water from a drinking water system (DWS) containing 6 µg/L (0.0000881 mM) of arsenic was used as a basis for microcosm experiments. Tolerance for As(III) of selectively isolated bacteria was checked based on optical density (OD) measurements which revealed As tolerance of Acidovorax facilis and Pseudomonas extremaustralis strains even at 25.38 mM of As(III). Compared to the control samples, the cell count values of the treated microcosms (DAS1_1, DAS2_1, DAS3_1 and DAS1_2, DAS2_2, DAS3_2) were higher by at least one or two orders of magnitude, even though, diversity indices, calculated from the NGS analysis, decreased. Results of NGS have shown that the structure of the bacterial community has changed as an effect of arsenic: representatives of Planctomycetes (3–6%) decreased while there was a proportional increase in the abundance of Proteobacteria (48–93%). Actinobacteria (2–8%), Bacteroidetes (4–7%), Patescibacteria (3–10%) and Verrucomicrobia (2–4%) were also abundant in the As(III)-treated microcosms. Phyla that contributes to less than 1% of the samples have disappeared already at 2.03 mM of As(III). The most frequent Archaea belonged to Nanoarchaeota (Woesearchaeia) (22–57%) and Thaumarchaeota (Nitrosopumilaceae) (9–40%) in both the control samples and microcosms with 2.03–23.38 mM of As(III). The cell structure of tested bacteria showed partial destruction after As(III) exposure. The ecotoxicological test revealed a cytotoxic effect above 6.67 mM concentration of As(III), genotoxicity could not be proven. Even though, microbes in our microcosms can be characterized by As(III) resistance and show measurable reactions to high As(III) concentrations, low concentrations of arsenic also trigger changes in the composition and diversity indices of the microbial communities. It is possible to cultivate arsenic tolerant microorganisms utilizing As(III) up to 25.38 mM to use them in bioremediation procedures contributing to water-purification processes.
{"title":"The Acute Impact of Arsenic As(III) on the Prokaryotic Community Composition and Selected Bacterial Strains Based on Microcosm Experiments","authors":"R. Farkas, M. Toumi, Gorkhmaz Abbaszade, K. Bóka, K. Takáts, E. Tóth","doi":"10.1080/01490451.2023.2181469","DOIUrl":"https://doi.org/10.1080/01490451.2023.2181469","url":null,"abstract":"Abstract In the present study, a 3-week microcosm experiment was conducted to assess the acute effect of arsenic trioxide [As(III)] at various concentrations (2.03, 4.06, 7.61, and 25.38 mM) on the bacterial communities. Water from a drinking water system (DWS) containing 6 µg/L (0.0000881 mM) of arsenic was used as a basis for microcosm experiments. Tolerance for As(III) of selectively isolated bacteria was checked based on optical density (OD) measurements which revealed As tolerance of Acidovorax facilis and Pseudomonas extremaustralis strains even at 25.38 mM of As(III). Compared to the control samples, the cell count values of the treated microcosms (DAS1_1, DAS2_1, DAS3_1 and DAS1_2, DAS2_2, DAS3_2) were higher by at least one or two orders of magnitude, even though, diversity indices, calculated from the NGS analysis, decreased. Results of NGS have shown that the structure of the bacterial community has changed as an effect of arsenic: representatives of Planctomycetes (3–6%) decreased while there was a proportional increase in the abundance of Proteobacteria (48–93%). Actinobacteria (2–8%), Bacteroidetes (4–7%), Patescibacteria (3–10%) and Verrucomicrobia (2–4%) were also abundant in the As(III)-treated microcosms. Phyla that contributes to less than 1% of the samples have disappeared already at 2.03 mM of As(III). The most frequent Archaea belonged to Nanoarchaeota (Woesearchaeia) (22–57%) and Thaumarchaeota (Nitrosopumilaceae) (9–40%) in both the control samples and microcosms with 2.03–23.38 mM of As(III). The cell structure of tested bacteria showed partial destruction after As(III) exposure. The ecotoxicological test revealed a cytotoxic effect above 6.67 mM concentration of As(III), genotoxicity could not be proven. Even though, microbes in our microcosms can be characterized by As(III) resistance and show measurable reactions to high As(III) concentrations, low concentrations of arsenic also trigger changes in the composition and diversity indices of the microbial communities. It is possible to cultivate arsenic tolerant microorganisms utilizing As(III) up to 25.38 mM to use them in bioremediation procedures contributing to water-purification processes.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"413 - 426"},"PeriodicalIF":2.3,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44645504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-16DOI: 10.1080/01490451.2023.2176574
H. Behzadipour, A. Sadrekarimi
Abstract Safe storage of mine tailings has challenged engineers as shown by numerous historical tailings dam failures. While the storing of tailings behind tailings dams is the most practical containment solution, poor mechanical characteristics (low strength and high compressibility) of these waste materials raises serious concerns regarding the stability of tailings dams. In this study, the microbially-induced calcite precipitation (MICP) technique is used to treat gold tailings. Accordingly, tailings were enriched with Sporosarcina pasteurii and flushed with different concentrations of cementation solution to find the CaCl2 amount that produces the highest shear strength in studied tailings. Their shear strength and compressibility were measured in direct shear and one-dimensional oedometer tests and compared with those of the untreated tailings. Results showed that the 50 mM CaCl2 cementation solution proved the most effective treatment solution with respect to MICP, reducing compressibility of tailings by about 300% when loaded up to 800 kPa and improving shear strength by 140%. X-Ray Diffraction (XRD) analysis and Scanning Electron Microscope (SEM) images of treated samples further illustrated the effects of MICP on the composition and structure of the tailings specimens.
{"title":"Bio-Assisted Improvement of Shear Strength and Compressibility of Gold Tailings","authors":"H. Behzadipour, A. Sadrekarimi","doi":"10.1080/01490451.2023.2176574","DOIUrl":"https://doi.org/10.1080/01490451.2023.2176574","url":null,"abstract":"Abstract Safe storage of mine tailings has challenged engineers as shown by numerous historical tailings dam failures. While the storing of tailings behind tailings dams is the most practical containment solution, poor mechanical characteristics (low strength and high compressibility) of these waste materials raises serious concerns regarding the stability of tailings dams. In this study, the microbially-induced calcite precipitation (MICP) technique is used to treat gold tailings. Accordingly, tailings were enriched with Sporosarcina pasteurii and flushed with different concentrations of cementation solution to find the CaCl2 amount that produces the highest shear strength in studied tailings. Their shear strength and compressibility were measured in direct shear and one-dimensional oedometer tests and compared with those of the untreated tailings. Results showed that the 50 mM CaCl2 cementation solution proved the most effective treatment solution with respect to MICP, reducing compressibility of tailings by about 300% when loaded up to 800 kPa and improving shear strength by 140%. X-Ray Diffraction (XRD) analysis and Scanning Electron Microscope (SEM) images of treated samples further illustrated the effects of MICP on the composition and structure of the tailings specimens.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"360 - 371"},"PeriodicalIF":2.3,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41953607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-15DOI: 10.1080/01490451.2023.2176573
P. A. Wani, Adebimpe Nusirat Ebudola, Y. K. Olusebi, N. Rafi, Sanusi Jadesola Fawzhia O, Oyelami Isaac Oluwaseun
Abstract Petroleum contamination to our biosphere particularly soil and water is due to release of petroleum products from petrochemical industries and is now a day’s biggest problem to the mankind. Present work was conducted to assess capability of hydrocarbon-utilizing and metal tolerant bacteria to degrade hydrocarbons and remediate metals in the soil contaminated with petroleum with the aim to form super bio-inoculants to restore soil to its original state. In order to achieve above goal, petroleum contaminated soil was observed for hydrocarbons using GC-MS, a total of 25 hydrocarbons were found in petroleum polluted soil with hydrocarbon concentration of 3738 mg/kg. Petroleum polluted soil was also observed for total bacterial population which was observed as 2.10 × 106 CFU/g of soil. Strains YSA-7 and YSA-8 showed highest potential for hydrocarbon-utilization with an absorbance of 0.681 and 0.614 respectively. Strains were also highly tolerant to Cu and Zn with tolerance of 600 µg/ml (Cu) and 1500 µg/ml (Zn) by strain YSA-7 and 700 µg/ml (Cu) and 1800 (Zn) µg/ml by strain YSA-8. Strains YSA-7 and YSA-8 were identified by 16 SrRNA as Lysinibacillus fusiformis and Stenotrophomonas maltophilia, respectively. After 20 days of incubation, Lysinibacillus fusiformis strain YSA-7 and Stenotrophomonas maltophilia YSA8 degraded all twenty-five (25) compounds but there was complete degradation of 13 compounds by strain YSA-7 and 17 compounds by strain YSA-8 using GC-MS. Both strains (YSA-7 and YSA8) were significant and efficient for removing Cu and Zn using mechanism of biosorption at different pH, metal concentrations and time of incubation. Remediation of hydrocarbons and metals under in vitro conditions suggests that the inoculation of such bacteria to petroleum contaminated soil possessing mixture of contaminants such as hydrocarbons and metals can remove such pollutants from contaminated environment and can restore such habitat to its original state.
{"title":"Hydrocarbon Utilizing and Metal Tolerant Bacteria Simultaneously Degrade Hydrocarbons and Detoxify Metals in Petroleum Contaminated Soil","authors":"P. A. Wani, Adebimpe Nusirat Ebudola, Y. K. Olusebi, N. Rafi, Sanusi Jadesola Fawzhia O, Oyelami Isaac Oluwaseun","doi":"10.1080/01490451.2023.2176573","DOIUrl":"https://doi.org/10.1080/01490451.2023.2176573","url":null,"abstract":"Abstract Petroleum contamination to our biosphere particularly soil and water is due to release of petroleum products from petrochemical industries and is now a day’s biggest problem to the mankind. Present work was conducted to assess capability of hydrocarbon-utilizing and metal tolerant bacteria to degrade hydrocarbons and remediate metals in the soil contaminated with petroleum with the aim to form super bio-inoculants to restore soil to its original state. In order to achieve above goal, petroleum contaminated soil was observed for hydrocarbons using GC-MS, a total of 25 hydrocarbons were found in petroleum polluted soil with hydrocarbon concentration of 3738 mg/kg. Petroleum polluted soil was also observed for total bacterial population which was observed as 2.10 × 106 CFU/g of soil. Strains YSA-7 and YSA-8 showed highest potential for hydrocarbon-utilization with an absorbance of 0.681 and 0.614 respectively. Strains were also highly tolerant to Cu and Zn with tolerance of 600 µg/ml (Cu) and 1500 µg/ml (Zn) by strain YSA-7 and 700 µg/ml (Cu) and 1800 (Zn) µg/ml by strain YSA-8. Strains YSA-7 and YSA-8 were identified by 16 SrRNA as Lysinibacillus fusiformis and Stenotrophomonas maltophilia, respectively. After 20 days of incubation, Lysinibacillus fusiformis strain YSA-7 and Stenotrophomonas maltophilia YSA8 degraded all twenty-five (25) compounds but there was complete degradation of 13 compounds by strain YSA-7 and 17 compounds by strain YSA-8 using GC-MS. Both strains (YSA-7 and YSA8) were significant and efficient for removing Cu and Zn using mechanism of biosorption at different pH, metal concentrations and time of incubation. Remediation of hydrocarbons and metals under in vitro conditions suggests that the inoculation of such bacteria to petroleum contaminated soil possessing mixture of contaminants such as hydrocarbons and metals can remove such pollutants from contaminated environment and can restore such habitat to its original state.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"372 - 381"},"PeriodicalIF":2.3,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48599684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-09DOI: 10.1080/01490451.2023.2171164
S. U. U. Jamil, Saeed Ullah, S. Zada, Muhammad Rafiq, A. A. Shah, F. Hasan
Abstract In this study, bacterial isolates form Bat guano of a lime stone cave capable of producing antibiotics are reported. Screening of bacterial strains was carried out from top, middle, and bottom layer of the guano sample. Out of 20 isolated strains, three showed antimicrobial activity against all tested ATTC strain of common bacterial (both gram positive and gram negative) and fungal species. 16s RNA gene sequencing of the strain showing maximum activity revealed the isolated strain as Bacillus subtilis. Optimum production of antimicrobial compounds took place in TSB medium after 72 h of incubation at 30 °C. FTIR of crude extract showed chemical similarity with bacitracin. GCMS analysis of the crude extract revealed a range of antimicrobial and anticancer compounds. It is concluded that Bacillus subtilis strain isolated from Bat Guano is a potential source of novel antimicrobial compounds of broad-spectrum nature and have inhibitory effects against potential pathogens.
{"title":"Isolation of Antimicrobial Active Compounds Producing Bacillus subtilis Strain from Bat Guano of Kashmir Cave, Pakistan","authors":"S. U. U. Jamil, Saeed Ullah, S. Zada, Muhammad Rafiq, A. A. Shah, F. Hasan","doi":"10.1080/01490451.2023.2171164","DOIUrl":"https://doi.org/10.1080/01490451.2023.2171164","url":null,"abstract":"Abstract In this study, bacterial isolates form Bat guano of a lime stone cave capable of producing antibiotics are reported. Screening of bacterial strains was carried out from top, middle, and bottom layer of the guano sample. Out of 20 isolated strains, three showed antimicrobial activity against all tested ATTC strain of common bacterial (both gram positive and gram negative) and fungal species. 16s RNA gene sequencing of the strain showing maximum activity revealed the isolated strain as Bacillus subtilis. Optimum production of antimicrobial compounds took place in TSB medium after 72 h of incubation at 30 °C. FTIR of crude extract showed chemical similarity with bacitracin. GCMS analysis of the crude extract revealed a range of antimicrobial and anticancer compounds. It is concluded that Bacillus subtilis strain isolated from Bat Guano is a potential source of novel antimicrobial compounds of broad-spectrum nature and have inhibitory effects against potential pathogens.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"337 - 346"},"PeriodicalIF":2.3,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43528217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.1080/01490451.2023.2171165
A. Rai, N. Sharma, V. Singh, B. S. Dwivedi, J. Singh, P. Rai
Abstract Phosphorus (P) is a key nutrient required by plants for their growth and development. A large part of applied phosphatic fertilizer becomes unavailable due to its fixation in soil. In rhizosphere, fluorescent Pseudomonads are instrumental in phosphate solubilization. We isolated Pseudomonads from the rhizosphere of Aloe barbadensis (L.), and screened them for their plant growth promoting ability such as - solubilization of phosphate, auxin production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. A total of 307 fluorescent Pseudomonas were isolated and screened, of which, 24 isolates showed a strong phosphate solubilizing ability, ranging from 69.71 to 236.73 µg ml−1 . The Indole acetic acid (IAA) production by all 24 isolates varied from 28.44 to 151.99 µg ml−1. However, 17 isolates produced ACC-deaminase ranging from 8.19 to 10.27 µmol mg−1 h−1. Analyses of 16S rRNA gene and Pseudomonas-specific genes grouped the isolates in seven different species of fluorescent Pseudomonas. Genotypic analysis based on 16S rDNA-RFLP using restriction enzymes (HaeIII, AluI and MspI) and BOX-PCR generated unique genotype specific pattern. The results suggested that Pseudomonas diversity has no association with microenvironments and the sampling site of the plants. 16S rRNA gene-based diversity indices revealed great diversity among the fluorescent Pseudomonas recovered from the rhizospheric regions of the Aloe. Due to the above plant growth-promoting traits, fluorescent Pseudomonas can be exploited as bio-inoculants for crops, with minimal damage to the environment. GRAPHICAL ABSTRACT
{"title":"Study of Phosphate Solubilizing Fluorescent Pseudomonas Recovered from Rhizosphere and Endorhizosphere of Aloe barbadensis (L.)","authors":"A. Rai, N. Sharma, V. Singh, B. S. Dwivedi, J. Singh, P. Rai","doi":"10.1080/01490451.2023.2171165","DOIUrl":"https://doi.org/10.1080/01490451.2023.2171165","url":null,"abstract":"Abstract Phosphorus (P) is a key nutrient required by plants for their growth and development. A large part of applied phosphatic fertilizer becomes unavailable due to its fixation in soil. In rhizosphere, fluorescent Pseudomonads are instrumental in phosphate solubilization. We isolated Pseudomonads from the rhizosphere of Aloe barbadensis (L.), and screened them for their plant growth promoting ability such as - solubilization of phosphate, auxin production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. A total of 307 fluorescent Pseudomonas were isolated and screened, of which, 24 isolates showed a strong phosphate solubilizing ability, ranging from 69.71 to 236.73 µg ml−1 . The Indole acetic acid (IAA) production by all 24 isolates varied from 28.44 to 151.99 µg ml−1. However, 17 isolates produced ACC-deaminase ranging from 8.19 to 10.27 µmol mg−1 h−1. Analyses of 16S rRNA gene and Pseudomonas-specific genes grouped the isolates in seven different species of fluorescent Pseudomonas. Genotypic analysis based on 16S rDNA-RFLP using restriction enzymes (HaeIII, AluI and MspI) and BOX-PCR generated unique genotype specific pattern. The results suggested that Pseudomonas diversity has no association with microenvironments and the sampling site of the plants. 16S rRNA gene-based diversity indices revealed great diversity among the fluorescent Pseudomonas recovered from the rhizospheric regions of the Aloe. Due to the above plant growth-promoting traits, fluorescent Pseudomonas can be exploited as bio-inoculants for crops, with minimal damage to the environment. GRAPHICAL ABSTRACT","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"347 - 359"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46732280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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