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Leaf Geometric morphometrics among a natural population of Norway maple (Acer platanoidesL.) in Northern Algeria 阿尔及利亚北部挪威枫(Acer platanoidesL.)自然种群的叶片几何形态计量学
Pub Date : 2021-05-15 DOI: 10.46325/GABJ.V0I0.938
Rida Mohammed Mediouni, Sarra Said, F. Ilias, Gaouar Suheil Semir Bechir
Maple (Acer L.) is a diverse tree genera that includes more than a hundred of deciduous and evergreen species in Northern hemisphere,Acer platanoidesis a species from the maple’s genuswith an invasive aptitudes in Europe and North America,this specieshad never been recordedin North Africa andthe main aim of this work is to investigate the shape and size variability withina natural population in Northern Algeria. The study was carried out using a collection of multivariate, bivariate and univariate statistics, 303 A. platanoides leaves were includedin the analysis counting 2 taxa from8 countries. The analyzed data showssome very close results between Algerian and European A. platanoides, One Way ANOVA of size provided a significant p.value<0.001 between the three studied populations, the Bonferroni correction doesn’t show any significant p.values between Algerian and European A.platanoides but confirmed the difference of A. platanoidesssp turkestanicum from the others, linear regression of shape and size shows a significant p.value of <0.001 but a low negative coefficient of correlation r= -0.18 and a low coefficient of determination r2= 0.033, Principal component analysis (PCA)shows an inertia of 53.48% between the first two components and revealed three different forms, MANOVA based on shape data revealed a significant p.value <0.001 between groups of taxa, a Pillai trace of 1.108, and a Wilks lambda coefficient of 0.192, the closest squared Mahalanobis distance (d=8.01) was reported between Algerian and European A. platanoidespopulations while the largest (d=16.74) was scored between Algerian and Iranian populations, clustering using Kmeans was depending on both Elbow and Silhouette methods, the typical number of clusters according to the two methods was k=2,however, clustering doesn’t reveal any specific shape or group of leaves, the statistical analysis proved a small phenotypic plasticity between Algerian and European A. platanoidesleaves in terms of shape while size remain conserved between both populations, the provided statistical tools confirms the ability of A. platanoidestoshow an environmental adaptation additionally also approves A. platanoidessspturkestanicumas distinguished subspecies.
枫(Acer L.)是一种分布于北半球的多样乔木属,包括一百多种落叶和常绿树种,其中高原槭(Acer platanoidesis)是枫属的一种,在欧洲和北美具有入侵能力,在北非从未有过记录,本研究的主要目的是研究阿尔及利亚北部自然种群的形状和大小变异。采用多变量、双变量和单变量统计方法,对来自8个国家的2个类群的303片platanoides叶片进行分析。分析数据表明,阿尔及利亚和欧洲的platanoides之间的结果非常接近,单因素方差分析(One Way ANOVA)在三个研究种群之间提供了显著的p值<0.001,Bonferroni校正没有显示阿尔及利亚和欧洲的platanoides之间有显著的p值,但证实了platanoidesssp turkestanicum与其他种群之间的差异。形状和大小的线性回归显示p值<0.001,但负相关系数r= -0.18,决定系数r2= 0.033,主成分分析显示前两个成分之间的惯性为53.48%,呈现出三种不同的形式,基于形状数据的方差分析显示类群间的p值显著<0.001,Pillai迹线为1.108,Wilks λ系数为0.192。阿尔及利亚和欧洲platanoides种群之间的马氏距离平方最接近(d=8.01),而阿尔及利亚和伊朗种群之间的马氏距离平方最大(d=16.74),使用Kmeans聚类依赖于肘部和廓形方法,根据两种方法的典型聚类数为k=2,但聚类不显示任何特定的形状或组。统计分析结果表明,阿尔及利亚和欧洲的platanoidessspturkestanicuma的叶片在形状和大小方面存在着较小的表型可塑性,而在两个种群之间则保持着保守性,所提供的统计工具证实了platanoidessspturkestanicuma具有环境适应性的能力,同时也证实了platanoidessspturkestanicuma是platanoidessspturkestanicuma的区分亚种。
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引用次数: 0
Characterization and Morphological Typology Of Fig Variety (Ficus carica) In The Tlemcen Region 特莱姆森地区无花果品种(Ficus carica)的特征和形态类型
Pub Date : 2021-05-15 DOI: 10.46325/GABJ.V0I0.939
M. Ikram, Chahbar Mohammed, Ilias Faiza, S. Gaouar
The purpose of this study is to describe morpho-biometric and identify fig varieties in Tlemcen region, based on 33 morphological markers (24 qualitative and 9 quantitative traits). The samples collected are processed by the ImageJ software for taking measurements. The statistical analysis was carried out using the SAS version 9 software. The results of the descriptive analysis of morphological traits studied allowed us to describe each variety and estimated the variability associated with each character of the different varieties sampled. The results of the principal Component Analysis (PCA) allowed the distinction of nine groups. Comparison of the data collected for each two-to-two variety and the estimate of the square distance between them showed that there is a highly significant difference between all local varieties. Then the comparison between local varieties and imports allowed the population to be separated into 16 distinct groups. Statistical analysis identified skin color as the primary discriminating factor between local varieties followed by fruit length, apical branching and leaf count per shoot. Statistical analysis of qualitative and quantitative morphological data from the 33 characters in study is to be effective for the distinction of new local varieties. That said, morpho-biometric description and local variety identification must be verified by the molecular tool to embark on an effective program of management and genetic improvement of this important biological resource.
本研究利用33个形态标记(24个定性性状和9个数量性状)对特莱姆森地区无花果品种进行形态生物特征描述和鉴定。采集的样品经ImageJ软件处理后进行测量。采用SAS version 9软件进行统计分析。形态学特征的描述性分析结果使我们能够描述每个品种,并估计与不同品种的每个特征相关的变异。主成分分析(PCA)的结果允许区分9组。对各二对二品种采集的数据进行比较,并对它们之间的平方距离估计值进行比较,结果表明各地方品种之间存在极显著差异。然后将本地品种和进口品种进行比较,将种群分为16个不同的群体。统计分析表明,皮肤颜色是地方品种间的主要判别因素,其次是果实长度、顶端分枝和每枝叶数。对所研究的33个性状的定性和定量形态学资料进行统计分析,对地方新品种的鉴别是有效的。也就是说,形态生物特征描述和本地品种鉴定必须通过分子工具进行验证,以便对这一重要的生物资源进行有效的管理和遗传改良。
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引用次数: 0
Identification and characterization of Common Sage in the wilaya of Tlemcen 特莱姆森野地中普通鼠尾草的鉴定与特征
Pub Date : 2021-05-15 DOI: 10.46325/GABJ.V0I0.941
Achir Mohammed, Benserida yassine, Senhadji Alaa Abir zohor, Warda Taibi, S. Gaouar
As part of the study of the biodiversity of plant genetic resources, in general and medicinal plants, in particular, we are interested in the study of an aromatic plant of Mediterranean origin, the Salvia officinalis known under the name of Mramiya , plant used in traditional medicine. Due to the absence of ethnic data and studies of racial characterizations of this species in Algeria, it is useful to contribute to the morphometric study of the sage population in the Tlemcen region. A collection of 100 plants, spread over five localities in this wilaya, is the subject of this investigation. Twenty-three measurements and 16 phenotypic characters were selected for this study. The effect of the region was studied; this factor was found to have a significant effect on the studied traits. A principal component analysis (PCA) and multiple correspondences analysis (MCA) was performed on the phenotypic characteristics, which revealed inertias corresponding to 18. 93% and 14.439% , 14.02% and 11.59% respectively for the first two principal components. This analysis made it possible to establish remarkable phenotypic differences, which have implications to be considered in the characterization program of this plant. An extraction of essential oils was performed to get an idea of the yield potential of two regions. The hydrodistillation of the aerial part of the Officinal Sage in the two regions Marsa Ben M’hidi and Ghazaouet gives a yield of 16.42% and 14.36%, respectively.
作为植物遗传资源生物多样性研究的一部分,特别是一般和药用植物,我们对地中海起源的芳香植物——鼠尾草(Salvia officinalis)的研究很感兴趣,鼠尾草被称为Mramiya,是传统医学中使用的植物。由于阿尔及利亚缺乏该物种的种族数据和种族特征研究,因此有助于对特莱姆森地区鼠类种群的形态计量学研究。这次调查的对象是分布在这个村庄五个地方的100种植物。本研究选择了23个测量值和16个表型性状。研究了区域的影响;这一因素对所研究性状有显著影响。对表型特征进行主成分分析(PCA)和多重对应分析(MCA),发现对应于18的惯性。前两个主成分分别为93%和14.439%、14.02%和11.59%。这种分析使得建立显着的表型差异成为可能,这对该植物的表征程序具有重要意义。进行了精油的提取,以了解两个区域的产量潜力。在Marsa Ben M 'hidi和Ghazaouet两个地区对药用鼠尾草的空中部分进行加氢蒸馏,其产率分别为16.42%和14.36%。
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引用次数: 0
Effect of water stress on the behavior of six durum wheat genotypes in a semi-arid region of western Algeria 水分胁迫对阿尔及利亚西部半干旱区6个硬粒小麦基因型性状的影响
Pub Date : 2021-05-15 DOI: 10.46325/GABJ.V0I0.946
Aicha Fetat, Rida Mohammed Mediouni, Benseddik Benchohra, Abbache Abdelkader, Z. Mansour
Six genotypes of durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn.) were investigated under different watering regimes. Phenological and morphological parameters as well as some yield components were monitored. The results showed that: (1) the water deficit significantly affects the height of the plants, the length and the weight of the spikes. (2) Irrigation has a significant effect on all of the yield components. Doses of 39mm at tillering, 60mm at stem elongation and 30mm at heading stage achieved the best yields. (3) Earliness at heading and at maturity is an important factor in determining the adaptation to water stress and in obtaining a good yield. (4) Under favorable irrigation conditions precocious and semi-precocious selected varieties have been shown to be more productive and even adapted to the water stress of the region. (5) The productivity of the durum wheat crop is imperatively based on the choice of a tolerant genotype to the conditions of the region on the one hand and on the use of supplementary irrigation on the other hand, especially at the vegetative stage where the yield component is the most determining, in our case it seems to be the populating spike carried out at the booting stage.
硬粒小麦的6个基因型。硬质(Desf)。在不同的浇水制度下进行了研究。对其物候、形态参数及部分产量成分进行了监测。结果表明:(1)水分亏缺显著影响植株的株高、穗长和穗重。(2)灌溉对各产量要素均有显著影响。分蘖期用量为39mm,拔节期用量为60mm,抽穗期用量为30mm,产量最佳。(3)抽穗期早、成熟期早是决定水稻对水分胁迫的适应性和获得良好产量的重要因素。(4)在良好的灌溉条件下,早熟和半早熟品种的产量更高,甚至适应了该地区的水分胁迫。(5)硬粒小麦的产量一方面取决于对该地区条件的耐受性基因型的选择,另一方面取决于补充灌溉的使用,特别是在营养阶段,产量成分是最具决定性的,在我们的情况下,似乎是在孕穗期进行的群体抽穗。
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引用次数: 0
Phytochemical screening and evaluation of the antioxidant and antibacterial activity of ‎Atriplex halimus from two regions Algeria (El Oued and Tlemcen) 阿尔及利亚两个地区(El Oued和Tlemcen)的halimus的植物化学筛选及抗氧化和抗菌活性评价
Pub Date : 2021-05-15 DOI: 10.46325/gabj.v5i2.72
T. Chaouche, F. Haddouchi, Fayza Abbou, M. Aissaoui, Ouhiba Boudjemai, Imane Ghellai, Souad Senhadji
The purpose of this work is to study the antioxidant and antibacterial activity of two plants of the same species « Atriplex halimus » from two different regions El Oued and Tlemcen. The plant leaves were subjected to a one-hour reflux extraction in methanol/acetone (70/30: v/v). Qualitative phytochemical examination of the leaf extract showed the presence of alkaloids, tannins and flavonoids of varying intensity. Coumarins, terpenoids and saponins are absent in both plants. Qualitative analysis of total polyphenols, flavonoids and condensed tannins of extracts shows that Atriplex halimus from the El Oued region has a high content of total polyphenols (10.25 ± 1.17 mg GAE/g DW) and tannins (9.23 ±1.09 mg EC/g DW) compared to Tlemcen. However, the high flavonoid content presented by Atriplex halimus from the Tlemcen region (3.09 ± 0.13 mg EC/g DW). The evaluation of the antioxidant activity of the extracts was carried out by three methods: total antioxidant capacity, trapping of the free radical DPPH and iron reducing power, the results obtained show that the extract of Atriplex halimus from Tlemcen reveals an interesting activity compared to that of El Oued with a CI50 = 193.47 ± 1.79µg/mL. Evaluation of antibacterial activity showing that El Oued extract has activity against Salmonella typhimirium ATCC 13311, Enterobacter cloacae ATCC 13047, Staphylococcus aureus ATCC 6538 and Listeria monocytogenes ATCC 15313 with a CMI value of 5 mg/mL, 10 mg/mL and 2.5 mg/mL respectively. However, Atriplex halimus from Tlemcen does not reveal any antibacterial activity against the strains tested.
本研究的目的是研究来自El Oued和Tlemcen两个不同地区的同一物种“Atriplex halimus”的两种植物的抗氧化和抗菌活性。植物叶片在甲醇/丙酮(70/30:v/v)中回流提取1小时。叶提取物的定性植物化学检查显示存在不同强度的生物碱、单宁和黄酮类化合物。这两种植物都不含香豆素、萜类和皂甙。定性分析结果表明,El Oued地区的盐柳总多酚、总黄酮和缩合单宁含量均高于天青(10.25±1.17 mg GAE/g DW)和单宁(9.23±1.09 mg EC/g DW)。而来自特莱姆森地区的盐柳黄酮含量最高(3.09±0.13 mg EC/g DW)。通过总抗氧化能力、捕获自由基DPPH和铁还原能力三种方法对提取物的抗氧化活性进行了评价,结果表明,与El Oued相比,特勒姆森的Atriplex halimus提取物具有较好的抗氧化活性,其CI50 = 193.47±1.79µg/mL。抑菌活性评价表明,黄叶提取物对鼠伤寒沙门菌ATCC 13311、阴沟肠杆菌ATCC 13047、金黄色葡萄球菌ATCC 6538和单核增生李斯特菌ATCC 15313具有抑菌活性,CMI值分别为5 mg/mL、10 mg/mL和2.5 mg/mL。然而,来自特莱姆森的Atriplex halimus对所测试的菌株没有显示出任何抗菌活性。
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引用次数: 2
Floristic diversity and Ecology of EpilithicDiatoms of the ChottChergui wetland (North-West of Algeria) 阿尔及利亚西北部乔特切尔吉湿地表层硅藻的区系多样性和生态学
Pub Date : 2021-05-15 DOI: 10.46325/gabj.v5i2.85
M. Negadi, Ait Hammou Mohamed, M. Miara, Hamid Bendif, Petulia Blake
This study is a phytoecological approach aimed to improve the knowledge on diatoms and their ecology in the wetland of ChottChergui in the northwesternregionof Algeria.For this work, 60 samples of solid substrates containing the epilithic diatom flora were collected in the field using a subjective method. The identification of diatom taxa was carried out according to a standard protocol while using pertinentliteraturethat specialized guides. At the level of the 5 sampling sites, water physicochemical parameters that could influence the distribution of diatoms were measured. The results obtained made it possible to recognize 36 diatom taxa belonging to 12 families, of which, the most dominant were Naviculaceae, Surirellaceae and Bacillariaceae. The calculated biodiversity indices (Shannon’s H and equitability revealed a low taxonomic richness which will probably be explained by the dominance of the typical brackish water taxa such as Mastogloiabraunii, Campylodiscusclypeus ,and Navicymbulapusilla. We were also able to characterize the distribution of identified taxa according to the physicochemical parameters of each site. Furthermore, the attraction of the diatomic communities to saprobia shows the predominance of the two classes: α-mesosaprobic and β-mesosaprobe during the two seasons of fall and spring. Canonical correspondence analysis revealed that the distribution of diatom communities is positively correlated with conductivity and temperature. Some species like Navicymbulapusilla, show a significant resistance to anthropogenic eutrophication.
本研究是一项植物生态学方法,旨在提高对阿尔及利亚西北地区乔特切尔吉湿地硅藻及其生态学的认识。在这项工作中,采用主观方法在野外收集了60份含有附着硅藻菌群的固体基质样品。硅藻类群鉴定按照标准方案进行,同时参考相关文献进行专门指导。在5个采样点的水平上,测量了影响硅藻分布的水体理化参数。结果表明,鉴定出12科36个硅藻类群,其中以Naviculaceae、surirellacae和Bacillariaceae为主。计算的生物多样性指数(Shannon’s H)和平稳性显示了较低的分类丰富度,这可能是由于典型的半咸淡水类群如Mastogloiabraunii、Campylodiscusclypeus和Navicymbulapusilla占主导地位。我们还能够根据每个站点的理化参数来表征已鉴定的分类群的分布。此外,双原子群落对腐臭菌的吸引力在秋季和春季表现为α-mesosaprobic和β-mesosaprobe两类优势。典型对应分析表明,硅藻群落的分布与电导率和温度呈正相关。一些物种,如Navicymbulapusilla,对人为富营养化表现出显著的抵抗力。
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引用次数: 1
Aromatic and Medicinal plants: Virtues and development prospects 芳香与药用植物:优点与发展前景
Pub Date : 2021-05-15 DOI: 10.46325/GABJ.V0I0.927
R. Azzi, T. Chaouche, N. Belyagoubi-Benhammou, Nassim Djabou, S. Gaouar
This special issue aims to bring together knowledge related to research in the broad scientific field of plants. The aspects of medicinal and aromatic plants (MAPs) and plants in general covered include the study of the biological and chemical activities of extracts rich in phytochemicals, essential oils and secondary metabolites by in vitro and in vivo tests, ethnobotanical and ethnopharmacological studies, biodiversity, plants morphological and physiological characterizations. MAPs are defined as all raw organic materials ‎‎(trees, shrubs and herbs) whose active elements of their respective organs (fruits, ‎flowers, leaves, roots, stems, buds, bulbs) are likely to be used.‎ The range of plants applications is vast, since it affects the ‎medical sector (pharmaceutical industry, allopathy, phytotherapy, homeopathy, ‎aromatherapy), that of cosmetics and perfumes, chemistry (detergents, dyes, ‎varnishes, fireworks, etc.) and the agro-food sector (minimally processed products ‎such as infusion plants, spices and dry herbs, etc.).‎Ethnobotanical research estimates there are approximately 50 000 to 70 000 plant ‎species used in traditional and modern herbal medicine around the world (Schippmann et al., 2006). The total amount of MAPs used by the pharmaceutical, ‎cosmetic, chemical and food industries is very difficult to estimate. Globally, more ‎than 35 000 are used by the pharmaceutical, chemical or cosmetic industries according ‎to professionals.  There is growing interest by scientists and the World Health Organization in medicinal ‎plants in many countries, especially in developing countries where access to modern ‎health care is limited. This action involves evaluating their efficacy and harmlessness, ‎and ensuring the safety and standardization of their use. ‎ Plants in general arouse a growing interest of the largest research centres ‎at the international level and are increasingly sought after by the agro-forestry and pharmaceutical ‎industries at the international, regional and national levels and therefore offer real ‎opportunities for socio-economic development of countries.‎Algeria, due to its geographical location, benefits from a very diverse climate and ‎flora. The plants grow in abundance in the coastal, mountainous and also Saharan ‎regions, many of which are renowned for their agro-forestry importance, medicinal virtue and used in traditional ‎medicine in health care (human and animal) and for culinary and cosmetic purposes.‎ These plants are potential natural remedies that can be used as a curative and ‎preventive therapeutic means, but this practice remains limited to patients and ‎herbalists who still have this empirical know-how.‎ The richness of the Algerian flora is therefore indisputable. It contains a large number ‎of species classified according to their degree of rarity: 289 fairly rare species, 647 rare ‎species, 640 very rare species, 35 extremely rare species and 168 endemic species (FAO, 2012). This situation requires
本期特刊旨在汇集广泛的植物科学领域的相关研究知识。药用和芳香植物(MAPs)和一般植物的各个方面包括通过体外和体内试验研究富含植物化学物质、精油和次生代谢物的提取物的生物和化学活性、民族植物学和民族药理学研究、生物多样性、植物形态和生理特征。地图被定义为所有可能使用其各自器官(果实、花、叶、根、茎、芽、球茎)的活性成分的原始有机材料(树木、灌木和草本植物)。植物的应用范围是广泛的,因为它影响到医疗部门(制药工业、对抗疗法、植物疗法、顺势疗法、芳香疗法)、化妆品和香水、化学(洗涤剂、染料、清漆、烟花等)和农业食品部门(最低加工产品),如输液植物、香料和干草药等)。民族植物学研究估计,全世界约有5万至7万种植物用于传统和现代草药(Schippmann et al., 2006)。制药、化妆品、化工和食品行业使用的map总量很难估计。据专业人士称,在全球范围内,制药、化工或化妆品行业使用了超过35000个。科学家和世界卫生组织对许多国家的药用植物越来越感兴趣,特别是在获得现代卫生保健的机会有限的发展中国家。这一行动包括评估其有效性和无害性,并确保其使用的安全性和标准化。总的来说,植物引起了国际上最大的研究中心日益增长的兴趣,并日益受到国际、区域和国家各级农林业和制药行业的追捧,因此为各国的社会经济发展提供了真正的机会。阿尔及利亚,由于其地理位置,受益于非常多样化的气候和植物群。这些植物在沿海、山区和撒哈拉地区大量生长,其中许多因其农林业重要性、药用价值和用于(人类和动物)保健的传统医学以及烹饪和美容目的而闻名。这些植物是潜在的自然疗法,可以用作治疗和预防性治疗手段,但这种做法仍然局限于患者和仍然拥有这种经验知识的草药医生。因此,阿尔及利亚植物群的丰富是无可争辩的。它包含了大量根据其稀有程度分类的物种:相当稀有物种289种,稀有物种647种,非常稀有物种640种,极稀有物种35种和168种特有物种(FAO, 2012)。这种情况需要详细的清单,形态生物计量学,生化和遗传特征,以有效地管理这种潜力。阿尔及利亚草药医生最需要的map通常是自发的(野生的),这使得它们难以量化。植物部门的参与者非常多且多样化,他们隶属于几个部门(农业、林业、研究、工业、商业、金融等)。阿尔及利亚有近2689名草药医生在国家贸易登记中心(CNRC)注册,具有“贸易商”身份。这些专业人士在这个领域非常活跃,他们寻求客户的来源和供应。在收集和分配之间的界面,草药医生在阿尔及利亚国内市场药用植物的组织中起着核心作用。非正式用语非常普遍。在大多数情况下,出售的植物不受质量控制。其中一些即使是自然提取的,也可能是危险的;它们可能对病人构成真正的危险,因为它们可能含有卖家和病人都不知道的有毒成分。出于天真、无知或商业计算,草药销售商声称帮助治愈了一些被归类为无法治愈的疾病,包括癌症和神经系统疾病等疾病(Ilbert et al., 2016)。
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引用次数: 0
Morphometric characterization of plum (Prunus domestica.L) in the far west of Algeria 阿尔及利亚远西部李子(Prunus domestica.L)的形态计量学特征
Pub Date : 2021-05-15 DOI: 10.46325/gabj.v5i2.88
Sarra Selka, F. Ilias, M. Mahdad, Mohammed Chakib Sekkal, S. Gaouar
As part of the characterization morphometric and identification of 25 varieties of plum (Prunus domestica.L), we have undertaken prospecting and collection of plant material through five different agroecological regions from the far west of Algeria (Wilaya of Tlemcen and Wilaya of Naama). We studied 24 agromorphological characters, were the objects of a statistical study to determine phenotypic diversity of quantitative and qualitative traits. The results of the ANOVA, the Hierarchical Classification (CAH), Multiple Component Analysis (MCA) and Chi-Square Tests showed a clear distinction between the accessions, and significant phenotypic variability is recorded for the characteristics studied such as (length and width of leaves, time of start of fruit ripening, size of the fruit).
作为李(Prunus domestica.L) 25个品种的特征、形态计量学和鉴定的一部分,我们在阿尔及利亚遥远的西部5个不同的农业生态区(特莱姆森的维拉亚和纳马的维拉亚)进行了植物材料的勘探和收集。我们研究了24个农形态性状,作为统计研究的对象,以确定数量和质量性状的表型多样性。方差分析(ANOVA)、层次分类(CAH)、多组分分析(MCA)和卡方检验(Chi-Square)的结果表明,各品种间存在明显的差异,所研究的性状(叶片的长度和宽度、果实开始成熟的时间、果实的大小)存在显著的表型变异。
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引用次数: 0
Cytotoxic and antioxidant effect of the ethanolic extract of Citrullus colocynthis L. plant fruit 甜瓜植物果实乙醇提取物的细胞毒和抗氧化作用
Pub Date : 2021-05-15 DOI: 10.46325/gabj.v5i2.76
Samira Fetni, N. Bertella
Citrullus colocynthis L. of the Cucurbitaecea botanical family is a plant widely used in traditional medicine in Algeria. It seems empirically effective in the treatment of various diseases. The aim of the present research is to evaluate the antioxidant and cytotoxic effects, in vitro, of the ethanolic extract of C. colocynthis fruits. The quantitative analysis has shown that the ethanolic extract of C. colocynthis is rich in total polyphenols with a content of 443.62 ± 2.13 µg EAG/mg of extract. The results obtained showed a strong anti-free radical activity of the ethanolic extract of C.colocynthis exerted against the DPPH free radical scavenging effect (CI50 = 6.31 µg/ml) and highlighted a powerful ferric reducing antioxidant power (CI50 = 27.94 µg/ml). We should also note a good antioxidant activity against the OH radical, obtained with the concentration (IC50 = 67.13 µg/ml). Furthermore, the obtained results indicate that the treatment of the three cancer cell lines (HepG2, SH-SY5Y and Raw 264.7) with the different concentrations of the used extract reduced the number of cells in a dose-dependent way. Based on our results, we can consider that Citrullus colocynthis is a plant with a strong pharmacological power and can therefore be used in phytotherapy.
瓜科植物Citrullus colocynthis L.是阿尔及利亚传统医药中广泛使用的植物。从经验来看,它在治疗各种疾病方面似乎有效。本研究的目的是评价薏苡仁果实乙醇提取物的体外抗氧化和细胞毒作用。定量分析结果表明,薏苡米醇提物含有丰富的总多酚,含量为443.62±2.13µg EAG/mg。结果表明,草芹醇提物对DPPH自由基有较强的清除作用(CI50 = 6.31µg/ml),对铁离子有较强的还原能力(CI50 = 27.94µg/ml)。我们还应该注意到,该浓度对OH自由基具有良好的抗氧化活性(IC50 = 67.13µg/ml)。此外,所得结果表明,不同浓度的提取物对HepG2、SH-SY5Y和Raw 264.7三种癌细胞的作用均呈剂量依赖性减少细胞数量。根据我们的研究结果,我们可以认为西葫芦是一种具有很强药理作用的植物,因此可以用于植物治疗。
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引用次数: 1
Genetic diversity of Algerian sorghum (Sorghum bicolor (L.) Moench) landraces by using agro-morphological markers 阿尔及利亚高粱(sorghum bicolor, L.)的遗传多样性利用农业形态标记鉴定地方品种
Pub Date : 2021-05-15 DOI: 10.46325/GABJ.V0I0.942
Redha Ouldkiar, Kirouani Abderrezzak, Laouar Meriem, Nadjemi Boubekeur, A. Aissa
The present study was performed to assess the native Algerian sorghum germplasm,20 accessions originating from the South of Algeria and one introduced commercial hybrid were evaluated for 12quantitative agro-morphological markers. Analysis of variance revealed significant differences (p ˂ 0.05) between accessions for all characters. Comparison of means by Duncan’s least significant test separate accessions into several homogeneous groups. It was found that sorghum landrace Ai19 showed the highest scores of final height (317 cm) and biomass dry yield (38 tons), Ai13 showed shortest vegetative cycles by 75 days to 50% flowering and the highest exploration of water to accumulate dry matter. The first two-principle component showed together more than 76% of the total variation. Clustering analysis showed that the 20 accessions were divided into four groups, mainly differentiated by forage production, days to 50% of flowering, final height, tillering capacity and leaf characteristics. The current study demonstrates that the characterization of the entire collection revealed a great phenotypic variability within the accessions and showed those that have markers of agronomic interest. Agro-morphological traits were very practical in detecting variation between local and commercial hybrid sorghum. Landraces Ai19, Ai13 and Fr1, through their valuable agro-morphological markers, could be used in sorghum genetic breeding programs.
本研究对阿尔及利亚高粱本地种质资源进行了评价,对阿尔及利亚南部地区的20份材料和1份引进的商业杂交种进行了12个定量农业形态标记的评价。方差分析显示,各性状间差异显著(p小于0.05)。通过Duncan最不显著性检验将资料分成几个同质组。结果表明,高粱长白品种Ai19的终高(317 cm)和生物量干产量(38 t)得分最高,Ai13的营养循环最短,75 d至50%开花,对水分积累干物质的探索程度最高。前两个主成分加起来占总变异的76%以上。聚类分析表明,20份材料可分为4个类群,主要以产草量、开花天数至50%、末高、分蘖能力和叶片特征进行区分。目前的研究表明,整个收集的特征揭示了巨大的表型变异性,并显示了那些具有农艺兴趣标记的材料。农业形态性状在检测地方杂交高粱与商品杂交高粱之间的变异方面非常实用。地方品种Ai19、Ai13和Fr1具有重要的农业形态标记价值,可用于高粱遗传育种。
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引用次数: 0
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