Gene Reports最新文献

{"title":"The impact of hydrogen sulfide on mesenchymal stem cells in rats suffering from liver fibrosis via suppression of TGF-β signaling","authors":"Doha El-Sayed Ellakwa ,&nbsp;Seham Mohamed Saied El Nakeeb ,&nbsp;Sawsan Ahmed Abd El Mohsen","doi":"10.1016/j.genrep.2024.102056","DOIUrl":"10.1016/j.genrep.2024.102056","url":null,"abstract":"<div><div>Worldwide, liver fibrosis (LF) causes complications and has an elevated death rate. The prevalence of mesenchymal stem cell therapy (MSC) is a result of the lack of liver donors. In recent years, the study of stem cell therapy has advanced into a promising and cutting-edge field of study. The purpose of this study is to assess the possible value of in vitro preconditioning of bone marrow-derived mesenchymal stem cells (BMSCs) with sodium hydrogen sulfide (NaHS), which aims to encourage rats to benefit from stem cell therapy with carbon tetrachloride-induced liver fibrosis. Materials and Methods: Fifty male albino rats (6 weeks old &amp; 120–150 g) were divided equally into 5 groups (10 rats each); the 1st group served as a negative control, the 2nd group was a positive control, in which rats received 2 mL/kg CCl4 (1:1 corn oil) twice a week for five weeks, and the remaining three groups received, in addition to CCL4, a NaHS solution (10 μmol/kg) every 2 days for 6 weeks, one dose of BMSCs (3 × 10⁶ cells per rat) intravenously, and a single dose of BMSCs (3 × 10⁶ cells per rat) in culture with 200 μmol/L NaHS for 24 h. Quantitative gene expression of transforming growth factor-beta (TGF-β), Smad, collagen, α-SMA, MAPK, β-catenin, GSK-3B, and CBS was carried out using real-time polymerase chain reaction; whereas the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin were estimated using colorimetric analysis. Also, the relative expression of MAPK, β-catenin, and GSK-3B by Western blot was done. Histopathological analysis was used to gauge the progression of LF. Results: The liver fibrosis group exhibited significantly increased serum ALT and AST levels, along with decreased serum albumin levels, compared to controls. Additionally, compared to controls, there was a rise in the gene expression of TGF-β, Smad, collagen, α-SMA, MAPK, β-catenin, and GSK-3B, while the gene expression of CBS is decreased. The biochemical parameters indicated above were greatly improved by BMSCs pretreated with H2S, and the liver sections produced from this group demonstrated a notable improvement in histopathology. Conclusion: The study investigated and demonstrated how NaHS affected the efficacy of BMSC therapy in rats with CCl4-induced liver fibrosis.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102056"},"PeriodicalIF":1.0,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the efficacy of medicinal plants based on immunological biomarkers in the treatment of bacterial infections: Current status and future directions","authors":"Joefred Mbogho Abogo ,&nbsp;Cédric Sima Obiang ,&nbsp;Herman Begouabe ,&nbsp;Rick Léonid Ngoua Meye Misso ,&nbsp;Juliette Ornely Orango Bourdette ,&nbsp;Guy Roger Ndong Atome ,&nbsp;Louis Clément Obame Engonga ,&nbsp;Joseph Privat Ondo","doi":"10.1016/j.genrep.2024.102052","DOIUrl":"10.1016/j.genrep.2024.102052","url":null,"abstract":"<div><div>Nowadays, a variety of infectious and non-infectious disorders are treated with various plants. This paper seeks to provide a framework for assessing and tracking the management of bacterial infections using immunological indicators of inflammation (cytokines). Thus, it demonstrates the production of inflammatory markers, the regulation of these markers by various plant extracts, the elimination of the various bacteria causing bacterial infections by these plant extracts, and, of course, a current report on the consequences of bacterial infections and their resistance to antibiotics. It has been demonstrated that plant extracts have a variety of immunomodulatory effects <em>in vivo</em> in addition to having antibacterial efficacy against multiple bacterial strains. The potential efficacy of medicinal plant extracts in controlling immune system gene expression makes them a vital tool for the diagnosis, management, and prevention of bacterial infections. The beliefs provided in this study need further investigation and refinement using modern scientific and technological methods.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102052"},"PeriodicalIF":1.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of Spinal Muscular Atrophy carrier detection in Guangdong Province, China: Insights from quantitative polymerase chain reaction and multiplex ligation-dependent probe amplification analysis","authors":"Chenxia Xu ,&nbsp;Jianming Peng ,&nbsp;Xuewei Wu ,&nbsp;Shengping Xiao ,&nbsp;Sheng Zhang ,&nbsp;Miaoyuan Li","doi":"10.1016/j.genrep.2024.102055","DOIUrl":"10.1016/j.genrep.2024.102055","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to identify carriers of Spinal Muscular Atrophy (SMA) among 10,630 pregnant women in Guangdong Province and provide prenatal diagnoses for high-risk fetuses from carrier couples. The goal was to prevent the birth of children affected by SMA. We evaluated the effectiveness of quantitative PCR (qPCR) and multiplex ligation-dependent probe amplification (MLPA) in detecting deletions in the SMN1 gene, with MLPA as the reference standard.</div></div><div><h3>Methods</h3><div>Fluorescent qPCR was used for initial SMA carrier screening, followed by confirmatory testing with MLPA for all detected carriers.</div></div><div><h3>Results</h3><div>Of the 10,630 women screened, 219 were identified as carriers (2.06 % detection rate). This included 17 cases of heterozygous deletion of exon 7 (E7), 145 cases with deletions of both E7 and exon 8 (E8), and 57 cases of E8 deletion alone. The carrier rate for E7 heterozygous deletion was established at 1.5 %. Prenatal diagnosis for seven carrier couples revealed five fetuses as carriers and one affected by SMA. The diagnostic concordance between qPCR and MLPA was 100 %.</div></div><div><h3>Conclusion</h3><div>The combined use of qPCR and MLPA is vital in identifying SMA carriers, allowing for early diagnosis and informed reproductive decisions. The high sensitivity and specificity of qPCR, matching MLPA, demonstrate its value in clinical settings for SMA screening and prenatal diagnosis. Our findings emphasize the critical importance of selecting precise diagnostic methods to enhance clinical outcomes in genetic screening programs.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102055"},"PeriodicalIF":1.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of metabolomic and transcriptomic profiles elucidates the core role of glycerophospholipid metabolism in hypertension related to metabolic syndrome","authors":"Yongqiao Zhang ,&nbsp;Feng Wei ,&nbsp;Minna Tang ,&nbsp;Ningzhi Zhang ,&nbsp;Rui Ma ,&nbsp;Shan Yu ,&nbsp;Yanbin He ,&nbsp;Xuling Liu ,&nbsp;Jiaxin Gong ,&nbsp;Hongtao Shi ,&nbsp;Sisi Ning ,&nbsp;Yuhong Zhao ,&nbsp;Jialu Hu ,&nbsp;Zhifeng Yao ,&nbsp;Zhaoqiang Cui","doi":"10.1016/j.genrep.2024.102049","DOIUrl":"10.1016/j.genrep.2024.102049","url":null,"abstract":"<div><h3>Background</h3><div>Metabolic syndrome (MetS) represents a comprehensive framework that encompasses conditions such as diabetes, hypertension (HBP), obesity, and dyslipidemia. MetS without hypertension significantly contributes to the development of refractory hypertension. However, the underlying molecular regulatory mechanisms of hypertension associated with MetS remain incompletely elucidated. The objective of this study was to identify differences in plasma metabolome and leukocyte transcriptome profiles between patients with MetS comorbid HBP compared to those with MetS with hypertension and HBP alone.</div></div><div><h3>Methods</h3><div>In this study, whole blood samples were collected from 104 participants, including patients with HBP, MetS without hypertension (MS), and MetS with hypertension (MS-HBP). Ultra-high-performance liquid chromatography was employed to perform metabolomic analysis of plasma samples. Comparative analysis was conducted to identify distinct metabolites among the three groups, while MetaboAnalyst 5.0 was utilized for enrichment and pathway analysis. Clinical correlation analysis and receiver operating characteristic analyses were applied to select biomarker metabolites for MS-HBP. Transcriptomic profiling of white blood cells was performed to identify dysregulated genes and KEGG/GO pathways. Integrative analysis of metabolomics and transcriptomics was used to construct molecular interaction networks. Finally, blood pressure was monitored every 2 weeks during the treatment period for experimental validation.</div></div><div><h3>Results</h3><div>The metabolomic results indicated that lipid metabolic pathways, particularly glycerophospholipid metabolic pathways, played a crucial role in MS-HBP. The area under the curve of tryptophan-isoleucine for diagnosis in the MS-HBP group was 0.82. Tryptophyl-isoleucine demonstrated a negative correlation with both HbA1c and diastolic blood pressure. Transcriptomic analysis revealed the involvement of MS-HBP in numerous immune and inflammatory pathways. Furthermore, the integrated analysis of metabolomic and transcriptomic data emphasized the importance of glycerophospholipid metabolism in MS-HBP. PPC exhibited a comparable effect on lowering blood pressure in SHR rats.</div></div><div><h3>Conclusion</h3><div>The findings of this study demonstrated that glycerophospholipid metabolism plays a pivotal role in the pathophysiological processes of MS-HBP, providing novel insights into early clinical diagnosis and the optimization of therapeutic strategies.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102049"},"PeriodicalIF":1.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing (WGS) and genome mining of Streptomyces sp. AFD10 for antibiotics and bioactive secondary metabolites biosynthetic gene clusters (BGCs)","authors":"Adeela Fatima ,&nbsp;Muhammad Abbas ,&nbsp;Shahid Nawaz ,&nbsp;Yasir Rehman ,&nbsp;Shafiq ur Rehman ,&nbsp;Imran Sajid","doi":"10.1016/j.genrep.2024.102050","DOIUrl":"10.1016/j.genrep.2024.102050","url":null,"abstract":"<div><div>Genomic approaches are being frequently used as an advancement over the conventional drug screening strategies for exploring the medicinally useful microbial strains. This study reports the whole genome sequencing (WGS), and genome mining for antibiotics biosynthetic gene clusters (BGCs), of the desert originated <em>Actinobacteria</em> strain <em>Streptomyces</em> sp. AFD10. The genomic DNA was isolated and subjected to library preparation and sequencing on Illumina Hiseq 2500 platform. The raw sequence reads were analyzed for various quality parameters and assembled through SOAPdenovo (ver. 2). The assembled genome is composed of 467 scaffolds (<em>N</em><em>50</em> = 98,164) sizing around 7,891,201 bp with GC content of 72.6 %, and number of coding sequences 7246. The RAST (ver 2.0) annotation SEED Viewer subsystem overview of the genome showed the presence of 163 subsystems in the genome of <em>Streptomyces</em> sp. AFD10. The strain exhibited maximum ANI (average nucleotide identity) up to 97.79 % with <em>Streptomyces violaceoruber</em>. The AntiSMASH (version 4.2.0) analysis of assembled genome showed a total of 35 biosynthetic gene clusters (BGCs) related to the biosynthesis of antibiotics and secondary metabolites in the genome of <em>Streptomyces</em> sp. AFD10. The identified gene clusters were predicted to be involved in the biosynthesis of different structural classes of antibiotics such as, polyketides, tripeptides, terpenes, lantipeptides, lassopeptide and butyrolacton etc. Overall, the study revealed that the <em>Actinobacteria</em> strains originated from extreme environments such as deserts are a promising source of medicinally useful metabolites, and the genome mining approaches provide an alternative platform to explore these resources for the novel antibiotics discovery.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102050"},"PeriodicalIF":1.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association mapping and genomic prediction of water deficit stress tolerance indices in spring bread wheat","authors":"Ahmad MajidiMehr ,&nbsp;Mohammad Hadi Pahlavani ,&nbsp;Zakaria El Gataa ,&nbsp;Reza Amiri-Fahliani","doi":"10.1016/j.genrep.2024.102054","DOIUrl":"10.1016/j.genrep.2024.102054","url":null,"abstract":"<div><div>Genome-wide association mapping (GWAM) is crucial for identifying the genetic architecture of quantitative traits, such as drought tolerance indices in bread wheat. This study aims to identify Marker-Trait Associations (MTAs) and genes related to drought tolerance indices in wheat. Seven drought tolerance indices were calculated based on grain weight per spike under field drought stress (FDS) and field non-stress (FNS) conditions. These indices included mean productivity (MP), geometric mean productivity (GMP), relative drought index (RDI), stress tolerance index (STI), tolerance index (TOL), stress susceptibility index (SSI), and yield stability index (YSI). Genotyping of the samples was performed using single nucleotide polymorphism (SNP) markers. A total of 96 MTAs were identified for the studied indices and conditions FNS and FDS in this experiment, with a threshold of -log10p ≥ 3.0. These included FNS, FDS, GMP, MP, STI, SSI, RDI, and YSI, with 15, 11, 16, 16, 20, 6, 6, and 6 MTAs, respectively. The MTAs identified for the drought tolerance indices GMP, MP, and STI were located on chromosomes 2 A, 3B, and 6 A, respectively. Moreover, this study identified four genes related to the indices, namely “<em>TraesCS2B02G000100</em>,” “<em>TraesCS3A02G000100</em>,” “<em>TraesCS2B02G000200</em>,” and “T<em>raesCS4B02G000100</em>”. These genes play a crucial role in drought tolerance and can be utilized for marker-assisted selection to enhance drought tolerance wheat genotypes.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102054"},"PeriodicalIF":1.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of polymorphisms in CYP2C8 and CYP2C9 with susceptibility to type 2 diabetes mellitus in a Chinese population","authors":"Wensu Wang ,&nbsp;Li Jin ,&nbsp;Jianguo Shen ,&nbsp;Yi Zhang ,&nbsp;Rong Zhang","doi":"10.1016/j.genrep.2024.102053","DOIUrl":"10.1016/j.genrep.2024.102053","url":null,"abstract":"<div><h3>Aims</h3><div><em>CYP2C8</em> and <em>CYP2C9</em> are cytochrome P450 epoxygenases responsible for metabolizing arachidonic acid into epoxyeicosatrienoic acids (EETs). These EETs play a crucial role as lipid mediators with numerous beneficial effects in type 2 diabetes mellitus (T2DM). In this study, we aimed to investigate the association of <em>CYP2C8</em> and <em>CYP2C9</em> genetic variants with T2DM in a Chinese population.</div></div><div><h3>Methods</h3><div>We conducted genotyping for 9 tag single nucleotide polymorphisms (SNPs) in <em>CYP2C8</em> and 10 tag SNPs in <em>CYP2C9</em> based on HapMap Chinese and Japanese data. Subsequently, we genotyped these SNPs in a Chinese cohort comprising 3410 individuals with T2DM and 3401 healthy controls. Statistical analyses were performed to assess the association between these SNPs and T2DM.</div></div><div><h3>Results</h3><div>In our study population, we observed that rs1819173, located within the <em>CYP2C9</em> gene region, was significantly associated with T2DM. Notably, the presence of the A allele was found to be protective against T2DM, as indicated by an odds ratio of 0.840 (95 % confidence interval: 0.780–0.904, <em>P</em> = 3.04 × 10⁻⁶). Furthermore, specific haplotypes (GT and AT) involving rs2071426 and rs6583967 in <em>CYP2C8</em> exhibited associations with T2DM (<em>P</em> = 0.049 and 0.038, respectively). Subsequently, we conducted an analysis of the association between rs1819173 and non-alcoholic fatty liver disease (NAFLD), revealing a significant correlation (OR = 0.764, 95 % CI: 0.629–0.928, <em>P</em> = 0.007).</div></div><div><h3>Conclusion</h3><div>Our research identified a genetic variants rs1819173 within <em>CYP2C8</em> are significantly associated with T2DM susceptibility in the Chinese population.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102053"},"PeriodicalIF":1.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic diversity in Bangladeshi rice (Oryza sativa L.) varieties utilizing SSR markers","authors":"Shamsunnahar Mukta ,&nbsp;Md. Nazmul Islam Bappy ,&nbsp;Jubo Bhuiyan ,&nbsp;Fatama Tous Zohora ,&nbsp;Dilruba Afrin","doi":"10.1016/j.genrep.2024.102051","DOIUrl":"10.1016/j.genrep.2024.102051","url":null,"abstract":"<div><div>One of the most fundamental staple foods for the vast majority of people on earth is rice. Farmer-grown traditional rice types which are well-adapted to a wide range of agro-ecological environments provide rich genetic resources for future rice enhancement. The quantity of allelic and genetic variety available in germplasm is a precondition for crop development and conservation techniques under severe climate conditions. However, adoption of high-yielding cultivars has led to their fast loss. Thus, the study's objective was to use marker-assisted selection (MAS) to examine the molecular diversity of rice genotypes using Simple Sequence Repeat (SSR) markers. Eighteen elite rice (<em>Oryza sativa</em> L.) germplasms from Bangladesh were chosen for genetic diversity analysis by SSR marker analysis. PCR amplification of the alleles was carried out and Visualized by Polyacrylamide Gel Electrophoresis (PAGE) system. Four primer pairs that were dispersed across eighteen rice chromosomes were used to detect polymorphisms initially. One representative from each chromosome made up the microsatellite marker panel that was selected, which included RM60, RM163, RM259, and RM278. With an average of 4.75 alleles per locus, a total of 19 alleles were found. Among the four loci spanning eighteen rice accessions, there was a moderate degree of genetic diversity; the average value of these loci is 0.67, ranging from 0.59 to 0.77. With an average of 0.7166, the polymorphism information content (PIC) represents the allele diversity frequency among the varieties, ranging from 0.6481 to 0.8025. Based on PIC values, RM 60 was determined to be the most effective marker for genotype identification. Six clusters were found with a similarity coefficient of 48 % using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram and the broad range of dissimilarity values (0.25–0.1), indicating a high level of cultivar diversity. Using a molecular breeding program, the results of the genetic diversity will help to select parents for rice varieties that can withstand different biotic and abiotic stresses.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102051"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico identification of simple sequence repeat (SSR) markers and phylogenetic analysis from chloroplast genomes of the genus Bambusa","authors":"Swati Pandey,&nbsp;Atul Bhargava","doi":"10.1016/j.genrep.2024.102048","DOIUrl":"10.1016/j.genrep.2024.102048","url":null,"abstract":"<div><div>Microsatellites or simple sequence repeats (SSRs), are short DNA sequences composed of repetitive units typically 1 to 6 base pairs long. These highly variable sequences are found in both protein-coding and noncoding DNA regions and exhibit a high degree of polymorphism. In the present study, chloroplast genomes of five species of <em>Bambusa</em> viz. <em>B. bambos, B. multiplex, B. teres, B. vulgaris,</em> and <em>B. tulda</em>, along with three outgroup species viz. <em>Sorghum timorense, Hordeum vulgare</em>, and <em>Triticum aestivum</em> were retrieved from NCBI in FASTA and GenBank formats and screened for chloroplast SSRs (cpSSRs) using MISA Perl script. The sequence analysis of the chloroplast genomes of different species of <em>Bambusa</em> confirmed the presence of 144 cpSSRs. The number of cpSSRs varied among the five bamboo species, with <em>B. vulgaris</em> exhibiting the highest count (31) and <em>B. multiplex</em> showing the lowest (27). Tetranucleotides were the most observed repeats in <em>Bambusa</em>, followed by mono-, di- and trinucleotides. Of the 144 SSR markers identified, only 16 (11.11 %) were suitable for primer design since the remaining sequences failed to produce acceptable primer pairs. An unweighted pair-group method with an arithmetic mean (UPGMA) dendrogram was constructed to analyze the genetic relationships among the genotypes. This analysis revealed three distinct clusters among the genotypes. SSR markers were found to be informative in differentiating the species of <em>Bambusa</em> and were likely to find potential applications in genetic fingerprinting, population genetics, conservation, and phylogenetics.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102048"},"PeriodicalIF":1.0,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction of MYBL2 as a common regulator between AHR and RELA: Its relationship with lnc-UCC and lnc-HOTTIP in glioblastoma multiforme","authors":"Ramin Soltani ,&nbsp;Alireza Tabibkhooei ,&nbsp;Morteza Hadizadeh ,&nbsp;Sepideh Parvizpour ,&nbsp;Roohallah Mahdi Esferizi ,&nbsp;Sorayya Ghasemi","doi":"10.1016/j.genrep.2024.102046","DOIUrl":"10.1016/j.genrep.2024.102046","url":null,"abstract":"<div><div>This study aimed to validate the experimental gene expression level of a common gene in the Aryl Hydrocarbon Receptor (AHR) and RELA in glioblastoma multiforme (GBM) using bioinformatics.</div><div>RNAseq data was analyzed to identify differentially expressed genes (DEGs), and LncRNAs related to the regulation of the founded gene were identified by the RNA Interactome Database. Ten healthy controls and 28 GBM patients' tissue samples were gathered, and the change in gene expression levels was measured by real-time PCR.</div><div>The results showed that MYBL2 is a common regulator between the AHR and RELA, and the expression of MYBL2, lnc-UCC, and lnc-HOTTIP genes was significantly increased in the GBM group.</div><div>Lnc-UCC expression showed significant positive correlations with MYBL2 and lnc-HOTTIP expression levels. Further investigation into these genes and their regulatory mechanisms is need.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102046"},"PeriodicalIF":1.0,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}