Gene Reports最新文献_第6页

Molecular insights and growth type-specific expression of Lv14-3-3ζ in the Pacific white shrimp, Litopenaeus vannamei Lv14-3-3ζ在凡纳滨对虾（Litopenaeus vannamei）中的分子特征及生长类型特异性表达

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-25 DOI: 10.1016/j.genrep.2025.102386

Md Abu Hanif, Yusin Cho, Shaharior Hossen, Kang Hee Kho

The proteins of the 14-3-3 family participate in diverse biological events by regulating diverse cell signaling pathways and the formation of protein-protein interactions. To understand the potential growth regulatory role of the 14-3-3 gene, the cDNA encoding Lv14-3-3ζ was isolated and characterized, and its tissue and growth-specific expression was analyzed in the Pacific white shrimp, Litopenaeus vannamei. The Lv14-3-3ζ cDNA was 2260 bp in length, containing a 741 bp open reading frame (ORF) encoding 246 putative amino acid residues flanked by a 75 bp 5′ untranslated region (UTR) and a 1443 bp 3′ UTR. Structurally, Lv14-3-3ζ is intronless and binds C-RAF phosphopeptide and fusicoccin ligands. Although this protein is predicted to have protein-succinylysine desuccinylase activity and protein-specific binding activity based on gene ontology, Lv14-3-3ζ is a multifunctional protein that interacts with diverse known and unknown proteins. Lv14-3-3ζ showed differential expression in tissue samples of the Pacific white shrimp, with the highest abundance in the digestive gland (p < 0.001), followed by lower expression in the ovary, intestine, and muscle, and low levels in the heart, nerve cord, and gill. A decreasing expression pattern of Lv14-3-3ζ was exhibited by slow to rapid-growth individuals, whereas myosin heavy chain (MHC) showed an increasing expression trend. The results of the present study provide comprehensive information on Lv14-3-3ζ gene, its structure and highlight its potential as a growth regulator in the Pacific white shrimp.

14-3-3家族蛋白通过调节多种细胞信号通路和形成蛋白-蛋白相互作用参与多种生物事件。为了解14-3-3基因的潜在生长调控作用，分离并鉴定了Lv14-3-3ζ编码的cDNA，并对其在凡纳滨对虾（Litopenaeus vannamei）中的组织特异性和生长特异性表达进行了分析。Lv14-3-3ζ cDNA全长2260 bp，包含一个741 bp的开放阅读框（ORF），编码246个推测氨基酸残基，两侧是一个75 bp的5 ‘未翻译区（UTR）和一个1443 bp的3 ’未翻译区（UTR）。结构上，Lv14-3-3ζ是无内含子的，结合C-RAF磷酸肽和梭霉素配体。尽管基于基因本体预测该蛋白具有蛋白-琥珀酰氨酸脱琥珀酰酶活性和蛋白特异性结合活性，Lv14-3-3ζ是一种多功能蛋白，可与多种已知和未知蛋白相互作用。Lv14-3-3ζ在太平洋白虾的组织样本中表现出差异表达，在消化腺中表达量最高（p < 0.001），其次是卵巢、肠和肌肉，在心脏、神经索和鳃中表达量较低。低速到高速生长个体Lv14-3-3ζ表达呈下降趋势，而肌球蛋白重链（MHC）表达呈上升趋势。本研究结果提供了有关Lv14-3-3ζ基因及其结构的全面信息，并突出了其作为太平洋白虾生长调节剂的潜力。

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引用次数: 0

Investigating the expression of virulence genes pilA and csuD of Acinetobacter baumannii in patients' samples in Tehran, Iran 伊朗德黑兰病人样本中鲍曼不动杆菌毒力基因pilA和csuD表达的研究

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-24 DOI: 10.1016/j.genrep.2025.102392

Hadis Nazeri , Amin Talebi Bezmin Abadi , Abbas Ali Imani Fooladi , Farid Rahimi

Acinetobacter baumannii is a leading cause of hospital-acquired infections and characterized by multidrug resistance and different virulence mechanisms. We aimed to characterize the antibiotic susceptibility profiles of A. baumannii in clinical isolates collected between 2022 and 2024. We confirmed A. baumannii by polymerase chain reaction (PCR) targeting 16S rRNA in fifty samples which included tracheal aspirates, urine, blood, wound, sputum, bronchoalveolar lavage, and ascitic fluid. We tested antibiotic susceptibility according to the 2022 guidelines of the Clinical and Laboratory Standards Institute and screened for resistance genes bla_OXA-23-like, bla_OXA-24-like, and bla_OXA-58-like by PCR. We used reverse transcription quantitative PCR of 20 representative samples in duplicates to profile pilA and csuD expression, normalized to 16S rRNA. csuD expression was higher in the target group than in controls, while pilA was only slightly increased; however, neither difference was statistically significant (P = 0.123 and P = 0.554, respectively), showing no meaningful differences among groups. Our findings highlight the importance of pili and chaperone–usher genes (PilA and CsuD) as they function in biofilm formation and adherence. Continually surveying for resistance and virulence factors is essential for guiding treatment strategies against carbapenem-resistant A. baumannii.

鲍曼不动杆菌是医院获得性感染的主要原因，其特点是多重耐药和不同的毒力机制。我们的目的是表征2022年至2024年间收集的鲍曼不动杆菌临床分离株的抗生素敏感性特征。我们采用针对16S rRNA的聚合酶链反应（PCR）方法，在50份包括气管吸入物、尿液、血液、伤口、痰液、支气管肺泡灌洗液和腹水的样本中证实鲍曼不动杆菌。我们根据临床与实验室标准协会2022年指南进行抗生素敏感性检测，并通过PCR筛选耐药基因blaOXA-23-like、blaOXA-24-like和blaOXA-58-like。我们使用反转录定量PCR对20个有代表性的样本进行了重复，以分析pilA和csuD的表达，归一化为16S rRNA。靶组csuD表达高于对照组，而pilA表达仅轻微升高；但两组差异均无统计学意义（P = 0.123、P = 0.554），组间差异无统计学意义。我们的研究结果强调了菌毛和伴侣引导基因（PilA和CsuD）在生物膜形成和粘附过程中的重要性。持续调查耐药和毒力因素对指导抗碳青霉烯类鲍曼不动杆菌的治疗策略至关重要。

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引用次数: 0

A transcriptomic atlas of macauba palm reveals organ-specific gene expression and stress-related pathways 澳门棕榈的转录组图谱揭示了器官特异性基因表达和应激相关途径

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-20 DOI: 10.1016/j.genrep.2025.102390

Lucas Miguel de Carvalho , Bárbara Regina Bazzo , Camila Carlos-Shanley , Carlos Augusto Colombo , Gonçalo Amarante Guimarães Pereira , Marcelo Falsarella Carazzolle

The macauba palm (Acrocomia aculeata) is an emerging oilseed species with promising applications in biodiesel production, as well as in food and cosmetic industries. Native to the Neotropics, it is in the early stages of domestication and distributed across diverse environments and edaphoclimatic conditions. However, genomic studies of macauba are limited due to the scarcity of publicly available sequence data, as it is considered a non-model plant. In this study, we present an exploratory analysis of a transcriptome dataset comprising seven different organs (roots, bulbs, male and female flowers, leaves, leaf sheath, and fruits). A total of 22,703 transcripts were assembled into a single reference dataset. Of these, 9729 transcripts (42.85 %) were annotated using KEGG orthology. Gene expression profiling revealed 306, 32, 41, 67, 92, 158 and 916 organ-specific transcripts in leaves, leaf sheaths, bulbs, female flower, male flower, fruit and root, respectively. Comparative analysis with oil palm (Elaeis guineensis) and date palm (Phoenix dactylifera) revealed 55 gene families exclusive to macauba palm. In addition, 221 transcripts related to drought stress were identified through functional annotation and grouped into 112 gene families. Root libraries revealed 7091 fungal transcripts - approximately 3.9 % of all reads – mainly derived from arbuscular mycorrhizal fungi (AMF) Rhizophagus spp. These findings highlight the central role of signal transduction pathways in response to environmental stresses in macauba palm. The transcriptome dataset generated in this study provides a valuable genomic resource for future genotype-phenotype investigations in macauba palm. Furthermore, the presence of AMF-associated transcripts suggests a potentially important role for these symbiotic fungi in macauba palm growth and development.

macauba palm （Acrocomia aculeata）是一种新兴的油籽植物，在生物柴油生产以及食品和化妆品工业中具有广阔的应用前景。它原产于新热带地区，处于驯化的早期阶段，分布在不同的环境和气候条件下。然而，由于澳门巴被认为是一种非模式植物，因此由于缺乏公开可用的序列数据，对澳门巴的基因组研究受到限制。在这项研究中，我们对包含七个不同器官（根、球茎、雄花和雌花、叶、叶鞘和果实）的转录组数据集进行了探索性分析。总共22,703份转录本被组装成一个单一的参考数据集。其中，9729个转录本（42.85%）用KEGG同源法进行了注释。基因表达谱分析显示，叶片、叶鞘、鳞茎、雌花、雄花、果实和根中分别有306、32、41、67、92、158和916个器官特异性转录本。通过与油棕（Elaeis guineensis）和枣椰树（Phoenix dactylifera）的比较分析，发现了澳门棕榈特有的55个基因家族。此外，通过功能注释鉴定了221个与干旱胁迫相关的转录本，并将其归为112个基因家族。在澳门棕榈根库中发现7091个真菌转录本，约占所有转录本的3.9%，主要来自丛枝菌根真菌（AMF） Rhizophagus spp.这些发现强调了信号转导通路在澳门棕榈响应环境胁迫中的核心作用。本研究生成的转录组数据集为未来澳门棕的基因型-表型研究提供了宝贵的基因组资源。此外，amf相关转录本的存在表明这些共生真菌在澳门棕榈生长发育中具有潜在的重要作用。

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引用次数: 0

Whole-exome sequencing identifies a novel splicing variant within TBC1D32 causing dysmorphic features and growth hormone deficiency 全外显子组测序在TBC1D32中发现了一种新的剪接变异，导致畸形特征和生长激素缺乏

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-20 DOI: 10.1016/j.genrep.2025.102389

Milad Gholami , Mahshid Fattahi , Dorsa Rostampour , Saeedeh Sadat Mir-Abdolhagh , Maryam Maktabi , Soudeh Ghafouri-Fard

TBC1D32 gene has a role in ciliary function and Sonic Hedgehog signaling. Pathogenic variants within this gene have been associated with hypopituitarism, ciliopathy and a range of dysmorphic features. Here, we used whole exome sequencing to find the molecular basis of dysmorphic features in a patient who presented with craniofacial phenotypes, intellectual disability, and growth hormone deficiency. Our results show that the patient carried a novel variant in the intron 27 of TBC1D32 gene (c.3054-2A > T). Based on the ACMG criteria, it is likely pathogenic. Thus, the current study broadens the spectrum of TBC1D32-related phenotypes.

TBC1D32基因在纤毛功能和Sonic Hedgehog信号传导中发挥作用。该基因内的致病变异与垂体功能低下、纤毛病和一系列畸形特征有关。在这里，我们使用全外显子组测序来发现一个颅面表型、智力残疾和生长激素缺乏的患者畸形特征的分子基础。我们的研究结果表明，患者携带TBC1D32基因内含子27的新变体（c.3054-2A >； T）。根据ACMG的标准，它可能是致病性的。因此，本研究拓宽了tbc1d32相关表型的谱。

引用次数: 0

Genes to jaws: A systematic review uncovering the role of genetics in malocclusion 基因对颌骨：一个系统的回顾揭示了遗传学在错颌畸形中的作用

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-20 DOI: 10.1016/j.genrep.2025.102388

T.P. Chaturvedi , Riddhi Mishra , Vipul Kumar Sharma , A.V. Aparna , Sakshee Nagvanshi

Background

The etiology of malocclusion is multifactorial. The genetic basis of the development of malocclusion is well established in the literature. Therefore, this systematic review aims to evaluate the genetic basis underlying the development of malocclusion.

Material and methods

Several renowned electronic databases were searched with predefined eligibility criteria. Two independent reviewers performed the study selection and data extraction process. The JBI critical appraisal tool was used to evaluate the quality of the included studies.

Results

After a rigorous selection process, a total of 19 studies met all criteria and were included in the systematic review. We observed that the notable genes, such as MYO1H, ADAMTS1, DLX6, and VDR, have shown consistent associations with specific malocclusion subtypes.

Conclusion

The results of the present study pave the way for incorporating genetic insights into clinical orthodontics, particularly for conditions like primary failure of eruption and syndromic malocclusions.

背景：错牙合的病因是多因素的。在文献中，错牙合发育的遗传基础已经得到了很好的证实。因此，本系统综述旨在评估错颌畸形发展的遗传基础。材料和方法根据预先确定的合格标准检索几个著名的电子数据库。两名独立的审稿人进行了研究选择和数据提取过程。采用JBI关键评价工具评价纳入研究的质量。结果经过严格的筛选过程，共有19项研究符合所有标准，并被纳入系统评价。我们观察到值得注意的基因，如MYO1H， ADAMTS1， DLX6和VDR，已经显示出与特定错颌错亚型的一致关联。结论本研究的结果为将遗传学见解纳入临床正畸学铺平了道路，特别是对于原发性出牙失败和综合征性错牙合等情况。

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引用次数: 0

Impact of genetic variants TLR2 rs5743708 and rs3804099 on the susceptibility of individuals to severe coronavirus disease 2019 基因变异TLR2 rs5743708和rs3804099对个体对2019年严重冠状病毒病易感性的影响

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-20 DOI: 10.1016/j.genrep.2025.102384

Maryam Noroozi , Kamran Heidarnejad , Reza Shafiei , Reza Shahbazi , Kurosh Kalantar , Mona Fani

Background

Pattern recognition receptors (PRRs) of the innate immune system potentially detect pathogen-associated molecular patterns (PAMPs) following viral invasion. While some Toll-like receptors (TLRs) offer protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, various studies suggest that Toll-like Receptor 2 (TLR2) activity is linked to heightened inflammatory responses and severe disease outcomes.

Methods

This study investigated two TLR2 single-nucleotide polymorphisms (SNPs), rs5743708 and rs3804099, using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) in patients with mild and severe COVID-19.

Results

Genotypic and allelic analyses revealed no statistically significant differences between severity groups. However, rs3804099 showed a significant association with creatinine and sodium levels in severe COVID-19 patients—creatinine differed between CT and TT genotypes, and sodium between CT and CC genotypes. Previous evidence associates this polymorphism with proteinuria after kidney transplantation, suggesting a role in renal homeostasis.

Conclusion

Our findings raise the possibility that rs3804099 may serve as a genetic marker for susceptibility to renal dysfunction and electrolyte imbalance during COVID-19, extending the clinical relevance of TLR2 polymorphisms beyond inflammatory signaling.

先天免疫系统的模式识别受体（PRRs）可能在病毒入侵后检测病原体相关的分子模式（PAMPs）。虽然一些toll样受体（TLRs）可以预防严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）感染，但各种研究表明，toll样受体2 （TLR2）活性与炎症反应加剧和严重疾病结局有关。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对轻、重度COVID-19患者的TLR2单核苷酸多态性rss5743708和rs3804099进行检测。结果基因型分析和等位基因分析显示，严重程度组间差异无统计学意义。然而，rs3804099与COVID-19重症患者的肌酐和钠水平有显著相关性——CT和TT基因型之间的肌酐水平存在差异，CT和CC基因型之间的钠水平存在差异。先前的证据表明，这种多态性与肾移植后的蛋白尿有关，表明它在肾脏稳态中起作用。结论rs3804099可能作为COVID-19患者肾功能障碍和电解质失衡易感性的遗传标记，将TLR2多态性的临床意义扩展到炎症信号之外。

{"title":"Impact of genetic variants TLR2 rs5743708 and rs3804099 on the susceptibility of individuals to severe coronavirus disease 2019","authors":"Maryam Noroozi , Kamran Heidarnejad , Reza Shafiei , Reza Shahbazi , Kurosh Kalantar , Mona Fani","doi":"10.1016/j.genrep.2025.102384","DOIUrl":"10.1016/j.genrep.2025.102384","url":null,"abstract":"<div><h3>Background</h3><div>Pattern recognition receptors (PRRs) of the innate immune system potentially detect pathogen-associated molecular patterns (PAMPs) following viral invasion. While some Toll-like receptors (TLRs) offer protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, various studies suggest that Toll-like Receptor 2 (TLR2) activity is linked to heightened inflammatory responses and severe disease outcomes.</div></div><div><h3>Methods</h3><div>This study investigated two TLR2 single-nucleotide polymorphisms (SNPs), rs5743708 and rs3804099, using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) in patients with mild and severe COVID-19.</div></div><div><h3>Results</h3><div>Genotypic and allelic analyses revealed no statistically significant differences between severity groups. However, rs3804099 showed a significant association with creatinine and sodium levels in severe COVID-19 patients—creatinine differed between CT and TT genotypes, and sodium between CT and CC genotypes. Previous evidence associates this polymorphism with proteinuria after kidney transplantation, suggesting a role in renal homeostasis.</div></div><div><h3>Conclusion</h3><div>Our findings raise the possibility that rs3804099 may serve as a genetic marker for susceptibility to renal dysfunction and electrolyte imbalance during COVID-19, extending the clinical relevance of TLR2 polymorphisms beyond inflammatory signaling.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102384"},"PeriodicalIF":0.9,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acquisition of multiple drug resistance genes by a novel isolate of A. junii from plastic laden landfill soil through horizontal gene transfer 从垃圾填埋场填满塑料的土壤中通过水平基因转移获得一株新菌株的多重耐药基因

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-19 DOI: 10.1016/j.genrep.2025.102387

Kaustubh Jumle , Naveen Malik , Sudarshan Singh Lakhawat , Baljinder Singh , Himanshu Gogoi , Akhilesh Kumar , Pushpender Kumar Sharma

In the present study, we have investigated and compared metagenome sample of plastic-laden landfill soil with garden soil for the presence of antimicrobial resistance (AMR) genes. Interestingly, during the analysis, we identified several AMR genes that include sul1and sul2 encoding sulfonamide resistance, emr (A) and emr (33) encoding macrolide resistance, bla AQU-3 coding β lactam resistance, vanX (A) coding glycopeptide resistance and drfG coding diaminopyrimidine resistance in plastic-laden landfill soil, whereas no AMR genes could be annotated in the garden soil metagenome sample. Furthermore, screening of AMR phenotype from a plastic-laden landfill soil reveals a bacterium demonstrating resistance to different classes of antibiotics. The whole genome sequencing and analysis reveals its close similarity with Acinetobacter junii, the strain identified henceforth named A. junii JPR0524. Assembled genome demonstrated ∼40 % GC content, and a genome size of ∼3.5 Mbp. Interestingly, several AMR genes could be annotated in A. junii JPR0524 genome. We also predicted several horizontal gene transfer (HGT) sites in JPR0524 genome, noticeably many of these sites overlapped with the predicted AMR genes sites. In addition, insertion of phage DNA fragments was also predicted at one end of the assembled genome, presumably attributed to horizontal gene transfer. Altogether, it appears that strain A. junii JPR0524 might have acquired these multidrug resistance genes in landfill sites through the horizontal gene transfer as evident from identification of putative HGT site in its genome.

在本研究中，我们调查并比较了垃圾填埋场土壤和花园土壤的宏基因组样本中抗菌素耐药性（AMR）基因的存在。有趣的是，在分析过程中，我们发现了几个AMR基因，包括编码磺胺抗性的sul1和sul2，编码大环内酯抗性的emr (A)和emr(33)，编码β内酰胺抗性的bla aqu3，编码糖肽抗性的vanX (A)和编码二氨基嘧啶抗性的drfG，而在花园土壤宏基因组样本中没有AMR基因被注释。此外，从充满塑料的垃圾填埋场土壤中筛选AMR表型揭示了一种细菌对不同类别的抗生素表现出耐药性。全基因组测序和分析结果表明，该菌株与朱尼不动杆菌（a.j junii JPR0524）具有密切的相似性。组装的基因组显示出约40%的GC含量，基因组大小约为3.5 Mbp。有趣的是，几个AMR基因可以在刺桐JPR0524基因组中被注释。我们还预测了JPR0524基因组中的几个水平基因转移（HGT）位点，值得注意的是，这些位点中有许多与预测的AMR基因位点重叠。此外，噬菌体DNA片段的插入也被预测在组装基因组的一端，可能归因于水平基因转移。总之，juni菌株JPR0524可能通过水平基因转移在垃圾填埋场获得了这些多药耐药基因，这一点从其基因组中推定的HGT位点的鉴定中得到了证明。

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引用次数: 0

Genomic characterization and bioactive potential of a Kitasatospora sp. from the rhizosphere of Inga edulis 印加根际一株Kitasatospora sp.的基因组特征及其生物活性潜力

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-14 DOI: 10.1016/j.genrep.2025.102381

Rafael de Souza Rodrigues , Antonia Queiroz Lima de Souza , Jania Lilia da Silva Bentes , Elison de Souza Sevalho , Kamila Rangel Primo Fernandes , Anderson Nogueira Barbosa , Maria de Fátima Oliveira Almeida , Rafael Pinto e Souza , Roneres Deniz Barbosa , Jeferson Chagas da Cruz , Ivanildes dos Santos Bastos , Patrícia Puccinelli Orlandi , Gilvan Ferreira da Silva , Afonso Duarte Leão de Souza

The genus Kitasatospora (phylum Actinobacteria) includes over 44 recognized species, yet their potential to produce bioactive compounds remains largely unexplored. In this study, we report the isolation and characterization of Kitasatospora sp. LaBMicrA B282, demonstrating its distinctiveness as an actinomycete associated with the rhizosphere of Inga edulis in an Amazonian urban forest fragment. The strain is Gram-positive, aerobic, and forms brownish-white aerial and grayish-brown substrate mycelia on ISP2+starch medium. The BLAST analysis of the 16S rRNA gene (<99 %% identity) and low genomic relatedness (ANI: 82.86–83.42 %; dDDH2: 27.20–29.60 %; dDDH4: 27.1–27.2 %) support its classification as a distinct species within the genus. Genome mining via antiSMASH identified 63 putative biosynthetic gene clusters (BGCs), including clusters with 100 % similarity to those for geosmin, 2-methylisoborneol, and ε-poly-l-lysine. Bioactivity assays revealed that the aqueous supernatant fraction displayed antiplasmodial activity against Plasmodium falciparum W2 (IC₅₀ = 36.10 μg/mL), while the AcOEt/2-propanol fraction showed fungicidal activity against four pathogenic fungi. These results highlight the remarkable biosynthetic and therapeutic potential of Kitasatospora strains from underexplored environments. Our findings emphasize the importance of investigating microbial diversity in unique ecological niches like the Amazonian rhizosphere and reinforce the need to conserve these environments as reservoirs of novel bioactive compounds.

Kitasatospora属（放线菌门）包括超过44个已知的物种，但它们产生生物活性化合物的潜力在很大程度上仍未被开发。在这项研究中，我们报道了Kitasatospora sp. LaBMicrA B282的分离和鉴定，证明了它作为亚马逊城市森林片段中Inga edulis根际相关的放线菌的独特性。菌株为革兰氏阳性，需氧，在ISP2+淀粉培养基上形成褐白色和灰褐色的底物菌丝。16S rRNA基因的BLAST分析（<； 99%的同源性）和低基因组亲缘性（ANI: 82.86 - 83.42%; dDDH2: 27.20 - 29.60%; dDDH4: 27.1 - 27.2%）支持其作为属内独特物种的分类。通过反smash进行基因组挖掘，鉴定出63个假定的生物合成基因簇（bgc），其中包括与土臭素、2-甲基异龙脑和ε-聚赖氨酸具有100%相似性的簇。生物活性测定表明，水上清部分对恶性疟原虫W2 （IC₅₀= 36.10 μg/mL）具有抗疟原虫活性，而AcOEt/2-丙醇部分对四种病原真菌具有杀菌活性。这些结果突出了从未开发的环境中提取的Kitasatospora菌株具有显著的生物合成和治疗潜力。我们的研究结果强调了在亚马逊根际等独特生态位中研究微生物多样性的重要性，并强调了保护这些环境作为新型生物活性化合物储存库的必要性。

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引用次数: 0

Hyperglycemia elevates oxidative stress markers without triggering mitochondrial large-scale deletions in peripheral blood leukocytes of type 2 diabetes patients 高血糖会升高2型糖尿病患者外周血白细胞的氧化应激标志物，而不会引发线粒体大规模缺失

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-14 DOI: 10.1016/j.genrep.2025.102385

Tejas Tajane , Prafulla Ambulkar , Pranita Waghmare , Bharati Taksande , Jwalant Waghmare

Objective

This study aimed to assess whether hyperglycemia-mediated reactive oxygen species (ROS) can cause a long-range deletion in the mitochondrial genome.

Methods

We assessed oxidative damage byproducts, such as 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA), in blood serum samples using the Enzyme-linked Immunosorbent Assay (ELISA) method in diabetes and healthy cohorts. In addition, the long-range mtDNA deletion (ΔmtDNA⁴⁹⁷⁷) assay was used to evaluate mtDNA deletions in peripheral blood leucocytes of type 2 diabetes patients. The Receiver Operating Characteristic (ROC) curve was computed to analyse the relationship between oxidative markers in the study groups.

Result

Serum 8-OHdG (34.44 ± 1.17 ng/mL) and MDA (65.90 ± 3.15 μmol/L) levels were significantly higher (p < 0.0001) in the diabetic group compared to the healthy controls (18.72 ± 0.71 ng/mL and 43.60 ± 2.47 μmol/L, respectively). The prevalence of hypertension also differed significantly between groups (p < 0.0001) and appeared to be positively associated with elevated 8-OHdG levels. However, ΔmtDNA⁴⁹⁷⁷ was not detected in the peripheral blood leucocytes of either study group. The ROC curve analysis indicated that 8-OHdG exhibited greater diagnostic accuracy than MDA for distinguishing diabetic patients, demonstrating higher specificity and a larger area under the curve (AUC).

Conclusion

Hyperglycemia elevated oxidative markers but failed to induce ΔmtDNA⁴⁹⁷⁷ in peripheral blood leucocytes. There was no ostensible association between ΔmtDNA⁴⁹⁷⁷ and diabetes mellitus.

目的本研究旨在评估高血糖介导的活性氧（ROS）是否会导致线粒体基因组的远程缺失。方法采用酶联免疫吸附法（ELISA）检测糖尿病和健康人群血清中氧化损伤副产物8-羟基脱氧鸟苷（8-OHdG）和丙二醛（MDA）的含量。此外，采用远程mtDNA缺失（ΔmtDNA4977）检测方法评估2型糖尿病患者外周血白细胞mtDNA缺失情况。计算受试者工作特征（ROC）曲线，分析各研究组氧化标志物之间的关系。结果糖尿病组血清8-OHdG（34.44±1.17 ng/mL）和MDA（65.90±3.15 μmol/L）水平显著高于健康对照组（18.72±0.71 ng/mL和43.60±2.47 μmol/L）（p < 0.0001）。高血压患病率在两组之间也有显著差异（p < 0.0001），并且似乎与8-OHdG水平升高呈正相关。然而，在两个研究组的外周血白细胞中均未检测到ΔmtDNA4977。ROC曲线分析表明，8-OHdG对糖尿病患者的诊断准确率高于MDA，具有更高的特异性和更大的曲线下面积（AUC）。结论高血糖使外周血白细胞氧化标志物升高，但对ΔmtDNA4977无诱导作用。ΔmtDNA4977与糖尿病之间没有明显的联系。

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引用次数: 0

Zinc oxide nanoparticle conjugated with Lapatinib has apoptogenic effect on colon cancer cell line 氧化锌纳米颗粒结合拉帕替尼对结肠癌细胞系有凋亡作用

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports

Pub Date : 2025-11-13 DOI: 10.1016/j.genrep.2025.102383

Seyed Milad Mousavi Eshkelani , Riham Nasser , Fatemeh Ghaderibarmi , Fatemeh Mirzaee , Farideh Tahmoresi , Arash Davoudi , Hedyeh Bagheri Darestani , Nafiseh Khakzad , Diba Heidari , Sana Tarashandeh Hemmati , Shahrzad Aghajani , Taraneh Ghasemipour , Fahimeh Abedini Bajgiran , Ahmed Raheem Rayshan , Ali salehzadeh

Adjuvant chemotherapy for colon cancer in its advanced stages is not associated with much success. Nanoparticle-mediated delivery of anticancer drugs represents an innovative approach for efficient and specific targeting of cancer cells. In this work, ZnO NPs were functionalized with glucose and conjugated with Lapatinib. Next, their anticancer effects on colon cancer cells were investigated. Physicochemical properties of ZnO@Glu-Lapatinib NPs were analyzed by FT-IR, XRD, EDS, DLS, Zeta potential measurement, and SEM and TEM microscopy. Cell viability in the colon cancer (SW480) and normal (HEK293) cells treated with the NPs was determined and 50 % inhibitory concentration (IC₅₀) was calculated. Cell cycle and apoptosis/necrosis analyses in the cancer cells were performed by flow cytometry and the expression level of the CASP8 and CASP9 genes was investigated by real-time PCR. Hoechst staining was performed to see the morphology of apoptotic cells. The ZnO@Glu-Lapatinib NPs were correctly synthesized with a spherical shape, DLS of 872.4 nm, zeta potential of −39.2, without impurity and diameter of 43.7–63.8 nm in their dry form. The IC₅₀ of ZnO@Glu-Lapatinib NPs in the normal and cancer cell lines were 36.82 and 7.78 μg/mL, respectively. Treatment of cancer cells with ZnO@Glu-Lapatinib NPs increased cell apoptosis, mainly early apoptosis (74.3 %) and elevated cell cycle blockage at the G0/G1 phase (79.9 %). Alterations in nuclear morphology such as chromatin condensation and fragmentation in favor of apoptosis were evident in cancer cells treated with ZnO@Glu-Lapatinib NPs in Hoechst staining. The expression levels of the CASP8 and CASP9 in the treated cancer cells increased to 6.4 and 3.9 folds, respectively. ROS analysis showed a 50.9-fold increase in reactive oxygen species oxidative after treatment. This study exhibited that ZnO@Glu-Lapatinib is a potential anticancer candidate that reduces the viability of colon cancer cells by inducing apoptosis pathways.

晚期结肠癌的辅助化疗成功率不高。纳米颗粒介导的抗癌药物递送代表了一种有效和特异性靶向癌细胞的创新方法。在这项工作中，ZnO NPs被葡萄糖功能化并与拉帕替尼偶联。接下来，研究了它们对结肠癌细胞的抗癌作用。采用FT-IR、XRD、EDS、DLS、Zeta电位测量、SEM和TEM显微镜对ZnO@Glu-Lapatinib NPs的理化性质进行了分析。测定NPs作用于结肠癌（SW480）和正常（HEK293）细胞的细胞活力，并计算50%抑制浓度（IC50）。流式细胞术检测肿瘤细胞周期和凋亡/坏死，实时荧光定量PCR检测CASP8和CASP9基因的表达水平。采用Hoechst染色观察凋亡细胞形态。合成的ZnO@Glu-Lapatinib纳米粒子为球形，DLS为872.4 nm， zeta电位为- 39.2，无杂质，干态直径为43.7 ~ 63.8 nm。ZnO@Glu-Lapatinib NPs在正常和肿瘤细胞株中的IC50分别为36.82和7.78 μg/mL。ZnO@Glu-Lapatinib NPs治疗癌细胞增加了细胞凋亡，主要是早期凋亡（74.3%）和G0/G1期细胞周期阻滞（79.9%）。Hoechst染色显示，在ZnO@Glu-Lapatinib NPs处理的癌细胞中，核形态的改变，如染色质凝聚和碎片化，有利于细胞凋亡。CASP8和CASP9在处理后的癌细胞中的表达水平分别增加到6.4倍和3.9倍。活性氧分析显示，处理后活性氧增加50.9倍。该研究表明ZnO@Glu-Lapatinib是一种潜在的抗癌候选者，通过诱导凋亡途径降低结肠癌细胞的活力。

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