Pub Date : 2024-09-19DOI: 10.1016/j.genrep.2024.102037
Marzieh Izadifard , Mohammad Ahmadvand , Kamran Alimoghadam , Hossein Pashaiefar , Ghazal Seghatoleslami , Maryam Barkhordar , Marjan Yaghmaie
Acute graft-versus-host disease (aGVHD) is a common complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), a procedure intended to treat malignant and nonmalignant hematological illnesses. At this time, there are no laboratory tests that can accurately forecast the likelihood of developing aGVHD, a patient's response to treatment, or their chance of life. Encouragingly, specific microRNAs have demonstrated consistent connections with the onset, severity, and clinical consequences of aGVHD through their dysregulated expression patterns, providing potential advantages in patient management and individualized therapy plans. Additionally, minimally invasive diagnostic methods are made easier by identification and measurement of circulating microRNAs as biomarkers in bodily fluids such as blood or urine using high-throughput screening techniques like qPCR, microarray analysis, and next-generation sequencing. Regarding the diagnosis, prognosis, and therapeutic response, this review is concentrated on the most recent research that offer proof of the possible utility of circulating microRNAs in blood and other bodily fluids as minimally invasive biomarkers of aGVHD. In addition to being useful tools for enhancing aGVHD management and patient outcomes, microRNAs distinctive expression patterns and functions in disease biology also present prospects for personalized medicine.
{"title":"Circulating microRNAs as the achilles forte of diagnostics and prognostics in acute graft versus host disease following allogeneic hematopoietic stem cell transplantation","authors":"Marzieh Izadifard , Mohammad Ahmadvand , Kamran Alimoghadam , Hossein Pashaiefar , Ghazal Seghatoleslami , Maryam Barkhordar , Marjan Yaghmaie","doi":"10.1016/j.genrep.2024.102037","DOIUrl":"10.1016/j.genrep.2024.102037","url":null,"abstract":"<div><div>Acute graft-versus-host disease (aGVHD) is a common complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), a procedure intended to treat malignant and nonmalignant hematological illnesses. At this time, there are no laboratory tests that can accurately forecast the likelihood of developing aGVHD, a patient's response to treatment, or their chance of life. Encouragingly, specific microRNAs have demonstrated consistent connections with the onset, severity, and clinical consequences of aGVHD through their dysregulated expression patterns, providing potential advantages in patient management and individualized therapy plans. Additionally, minimally invasive diagnostic methods are made easier by identification and measurement of circulating microRNAs as biomarkers in bodily fluids such as blood or urine using high-throughput screening techniques like qPCR, microarray analysis, and next-generation sequencing. Regarding the diagnosis, prognosis, and therapeutic response, this review is concentrated on the most recent research that offer proof of the possible utility of circulating microRNAs in blood and other bodily fluids as minimally invasive biomarkers of aGVHD. In addition to being useful tools for enhancing aGVHD management and patient outcomes, microRNAs distinctive expression patterns and functions in disease biology also present prospects for personalized medicine.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102037"},"PeriodicalIF":1.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.1016/j.genrep.2024.102039
Abdullah Al Saba , Jasmin Nur , Md Sohrab Alam , Zakir Hossain Howlader , Laila N. Islam , A.H.M. Nurun Nabi
Host genetics among other factors play an important role in COVID-19 disease severity. TMPRSS2, a serine protease, facilitates the priming of the SARS-CoV-2 spike protein, which is essential for the virus to enter the host cell. Several studies had targeted the TMPRSS2 polymorphisms with respect to SARS-CoV-2 infection and COVID-19 disease severity. Initially, the Whole Exome Sequencing (WES) of 7 healthy individuals and 22 COVID-19 patients with different degrees of disease severity was conducted to find the mutational landscape in different genes. A total of 26 single nucleotide polymorphisms (SNPs) were identified of which six were within the exons of TMPRSS2 (four synonymous and two nonsynonymous variants) while the rest of the 20 SNPs were recognized within the flanking intronic regions of TMPRSS2 gene. The nonsynonymous SNPs, rs75603675 and rs12329760, were further evaluated for association with disease severity in a larger sample size of 120 individuals by PCR followed by Sanger sequencing. Neither allelic nor genotypic distributions of rs12329760 were significantly associated with COVID-19 disease severity. However, individuals harboring the A allele of rs75603675 was found to have higher risk of severe COVID-19 compared to the C allele [OR (95%CI): 1.95 (1.11–3.39), p = 0.019]. Also, the genotype A/A [OR (95%CI): 5.13 (1.00–26.38), p = 0.033] of rs75603675 was associated with increased risk of severe COVID-19 under the recessive genetic inheritance model. Although the impact of COVID-19 pandemic has waned due to vaccination and public health measures, continued research on the association of COVID-19 disease severity and infection susceptibility with host genetics is required to shed valuable insights on the future long-term health effects of COVID-19 and impact of new variants on different populations and enable implication of proper informed healthcare strategies during future public health crises.
{"title":"Missense variant rs75603675 within TMPRSS2 gene is associated with the increased risk of severe form of COVID-19","authors":"Abdullah Al Saba , Jasmin Nur , Md Sohrab Alam , Zakir Hossain Howlader , Laila N. Islam , A.H.M. Nurun Nabi","doi":"10.1016/j.genrep.2024.102039","DOIUrl":"10.1016/j.genrep.2024.102039","url":null,"abstract":"<div><div>Host genetics among other factors play an important role in COVID-19 disease severity. TMPRSS2, a serine protease, facilitates the priming of the SARS-CoV-2 spike protein, which is essential for the virus to enter the host cell. Several studies had targeted the TMPRSS2 polymorphisms with respect to SARS-CoV-2 infection and COVID-19 disease severity. Initially, the Whole Exome Sequencing (WES) of 7 healthy individuals and 22 COVID-19 patients with different degrees of disease severity was conducted to find the mutational landscape in different genes. A total of 26 single nucleotide polymorphisms (SNPs) were identified of which six were within the exons of TMPRSS2 (four synonymous and two nonsynonymous variants) while the rest of the 20 SNPs were recognized within the flanking intronic regions of TMPRSS2 gene. The nonsynonymous SNPs, rs75603675 and rs12329760, were further evaluated for association with disease severity in a larger sample size of 120 individuals by PCR followed by Sanger sequencing. Neither allelic nor genotypic distributions of rs12329760 were significantly associated with COVID-19 disease severity. However, individuals harboring the A allele of rs75603675 was found to have higher risk of severe COVID-19 compared to the C allele [OR (95%CI): 1.95 (1.11–3.39), <em>p</em> = 0.019]. Also, the genotype A/A [OR (95%CI): 5.13 (1.00–26.38), <em>p</em> = 0.033] of rs75603675 was associated with increased risk of severe COVID-19 under the recessive genetic inheritance model. Although the impact of COVID-19 pandemic has waned due to vaccination and public health measures, continued research on the association of COVID-19 disease severity and infection susceptibility with host genetics is required to shed valuable insights on the future long-term health effects of COVID-19 and impact of new variants on different populations and enable implication of proper informed healthcare strategies during future public health crises.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102039"},"PeriodicalIF":1.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-16DOI: 10.1016/j.genrep.2024.102034
Basireh Baei , Ezzat Allah Ghaemi , Naeme Javid
Background
Silver nanoparticles (AgNPs) exhibit a dose-dependent anti-bacterial effect, and it was aimed in this study to investigate the impact of sub-minimum inhibitory concentration (MIC) doses of AgNPs on the expression of virulence genes in Staphylococcus aureus (S. aureus).
Methods
Minimum inhibitory concentration (MIC) values for AgNPs were determined for 183 S. aureus isolates. Gene expression was assessed in 14 isolates with sea and seb genes treated with AgNPs at a sub-MIC dose of 1 μg/ml. Accordingly, these strains were exposed to 1 μg/ml doses of AgNPs, and gene expression levels of sea, seb, and agr were assessed using quantitative RT-PCR after 4- and 12-hour post-AgNPs inoculation at 37 °C. The impact of AgNPs on the virulence factors of S. aureus was investigated over different time points, focusing on methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolates.
Results
Analysis revealed significant reductions in gene expression levels of seb and agr after 4 h post-AgNPs treatment in the MSSA group (p < 0.05), with further decreases observed at 12 h for sea, seb, and agr genes (p < 0.0001). MRSA isolates exhibited significant declines in sea and agr gene expression levels at both time points (p < 0.0001). However, no significant changes were observed in seb gene expression among MRSA isolates. Fold-change analysis indicated time-dependent effects of AgNP treatment on gene expression, highlighting substantial alterations in gene expression levels over time, particularly in seb and agr genes.
Conclusion
These results show that sub-MIC levels of AgNPs greatly decrease the gene expression of important virulence factors in MSSA and MRSA strains, indicating their promise as treatments for S. aureus infections, particularly at 12 h post-treatment. The differential response between MSSA and MRSA isolates highlights the importance of strain variation in antimicrobial strategies.
{"title":"Modulatory effects of sub-MIC concentrations silver nanoparticles on virulence factor gene expression in Staphylococcus aureus","authors":"Basireh Baei , Ezzat Allah Ghaemi , Naeme Javid","doi":"10.1016/j.genrep.2024.102034","DOIUrl":"10.1016/j.genrep.2024.102034","url":null,"abstract":"<div><h3>Background</h3><p>Silver nanoparticles (AgNPs) exhibit a dose-dependent anti-bacterial effect, and it was aimed in this study to investigate the impact of sub-minimum inhibitory concentration (MIC) doses of AgNPs on the expression of virulence genes in <em>Staphylococcus aureus</em> (<em>S. aureus</em>).</p></div><div><h3>Methods</h3><p>Minimum inhibitory concentration (MIC) values for AgNPs were determined for 183 <em>S. aureus</em> isolates. Gene expression was assessed in 14 isolates with <em>sea</em> and <em>seb</em> genes treated with AgNPs at a sub-MIC dose of 1 μg/ml. Accordingly, these strains were exposed to 1 μg/ml doses of AgNPs, and gene expression levels of <em>sea</em>, <em>seb</em>, and <em>agr</em> were assessed using quantitative RT-PCR after 4- and 12-hour post-AgNPs inoculation at 37 °C. The impact of AgNPs on the virulence factors of <em>S. aureus</em> was investigated over different time points, focusing on methicillin-sensitive <em>S. aureus</em> (MSSA) and methicillin-resistant <em>S. aureus</em> (MRSA) isolates.</p></div><div><h3>Results</h3><p>Analysis revealed significant reductions in gene expression levels of <em>seb</em> and <em>agr</em> after 4 h post-AgNPs treatment in the MSSA group (<em>p</em> < 0.05), with further decreases observed at 12 h for <em>sea</em>, <em>seb</em>, and <em>agr</em> genes (<em>p</em> < 0.0001). MRSA isolates exhibited significant declines in <em>sea</em> and <em>agr</em> gene expression levels at both time points (<em>p</em> < 0.0001). However, no significant changes were observed in <em>seb</em> gene expression among MRSA isolates. Fold-change analysis indicated time-dependent effects of AgNP treatment on gene expression, highlighting substantial alterations in gene expression levels over time, particularly in <em>seb</em> and <em>agr</em> genes.</p></div><div><h3>Conclusion</h3><p>These results show that sub-MIC levels of AgNPs greatly decrease the gene expression of important virulence factors in MSSA and MRSA strains, indicating their promise as treatments for <em>S. aureus</em> infections, particularly at 12 h post-treatment. The differential response between MSSA and MRSA isolates highlights the importance of strain variation in antimicrobial strategies.</p></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102034"},"PeriodicalIF":1.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Epstein-Barr virus (EBV) is a human oncogenic virus that targets B lymphocytes and is associated with some malignancies, such as Burkitt's lymphoma. Curcumin, as a natural product, has anticancer, antiproliferative, and antiviral properties. The combination of curcumin with some nanoparticles, such as dendrosom, can increase its solubility and anti-cancer effects. The present research aims to investigate the antiviral effect of Dendrosomal NanoCurcumin (DNC) on Burkitt's lymphoma cells (Daudi cell line) and the expression of EBV lytic genes and apoptosis.
Materials and methods
The cytotoxicity of curcumin, DNC, and dendrosome on Daudi cells and Peripheral Blood Mononuclear Cells (PBMC) as a control was assessed using a (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) MTT assay. Cell apoptosis was evaluated by Annexin/PI flow cytometry. RNA expression of BZLF1, BHRF1, and BRLF1 was assessed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay. The t-test was used for statistical analysis.
Results
The half-minimum concentration of cytotoxicity (CC50) for nanocurcumin was 30 μg/ml, for curcumin 50 μg/ml, and for dendrosome 987 μg/ml. DNC caused time and dose-dependent death in Daudi cancer cells compared to curcumin and showed no toxicity in control cells. Quantitative RT-PCR evaluation showed a significant decrease in BZLF1, BHRF1, and BRLF1 mRNA expression at 30 μg/ml concentration of DNC compared to the control. The data obtained from the relative measurement of gene expression showed a decrease in the expression of viral lytic genes (P < 0.001).
Conclusion
In this study, it is noted that carriers, such as dendrosome, enhance the bioavailability of curcumin, thereby increasing its antitumor properties and cell apoptosis. Noteworthy, DNC nanoparticles can serve as a promising drug candidate and supplement in the treatment of Burkitt's lymphoma and in improving patient survival by reducing the expression of EBV lytic phase viral genes.
{"title":"Inhibitory effect of dendrosomal-nanocurcumin on Burkitt's lymphoma cells by reducing EBV lytic gene expression","authors":"Mahboobeh Cheragh , Masoud Parsania , Majid Sadeghizadeh , Mohammad Hassan Pouriayevali","doi":"10.1016/j.genrep.2024.102035","DOIUrl":"10.1016/j.genrep.2024.102035","url":null,"abstract":"<div><h3>Introduction</h3><div>Epstein-Barr virus (EBV) is a human oncogenic virus that targets B lymphocytes and is associated with some malignancies, such as Burkitt's lymphoma. Curcumin, as a natural product, has anticancer, antiproliferative, and antiviral properties. The combination of curcumin with some nanoparticles, such as dendrosom, can increase its solubility and anti-cancer effects. The present research aims to investigate the antiviral effect of Dendrosomal NanoCurcumin (DNC) on Burkitt's lymphoma cells (Daudi cell line) and the expression of EBV lytic genes and apoptosis.</div></div><div><h3>Materials and methods</h3><div>The cytotoxicity of curcumin, DNC, and dendrosome on Daudi cells and Peripheral Blood Mononuclear Cells (PBMC) as a control was assessed using a (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) MTT assay. Cell apoptosis was evaluated by Annexin/PI flow cytometry. RNA expression of <em>BZLF1</em>, <em>BHRF1</em>, and <em>BRLF1</em> was assessed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay. The <em>t</em>-test was used for statistical analysis.</div></div><div><h3>Results</h3><div>The half-minimum concentration of cytotoxicity (CC50) for nanocurcumin was 30 μg/ml, for curcumin 50 μg/ml, and for dendrosome 987 μg/ml. DNC caused time and dose-dependent death in Daudi cancer cells compared to curcumin and showed no toxicity in control cells. Quantitative RT-PCR evaluation showed a significant decrease in <em>BZLF1</em>, <em>BHRF1</em>, and <em>BRLF1</em> mRNA expression at 30 μg/ml concentration of DNC compared to the control. The data obtained from the relative measurement of gene expression showed a decrease in the expression of viral lytic genes (<em>P</em> < 0.001).</div></div><div><h3>Conclusion</h3><div>In this study, it is noted that carriers, such as dendrosome, enhance the bioavailability of curcumin, thereby increasing its antitumor properties and cell apoptosis. Noteworthy, DNC nanoparticles can serve as a promising drug candidate and supplement in the treatment of Burkitt's lymphoma and in improving patient survival by reducing the expression of EBV lytic phase viral genes.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102035"},"PeriodicalIF":1.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study focuses on the phytochemical composition, in vitro and in silico antioxidant and NO reduction potential of Zingiber Officinale rhizome essential oil (ZOREO). The GC–MS analysis has revealed the presence of 47 volatile constituents representing 94.35 % of the total area of EO. The major compounds identified were α-zingiberene (20.58 %), geranial (10 %), β-sesquiphellandrene (7.39 %), and neral (7.23 %). The EO was rich in sesquiterpene hydrocarbons (45.93 %) followed by monoterpene hydrocarbons (29.29 %). The EO showed moderate antioxidant activity compared to the standard (IC50 = 5.16 ± 0.4 mg/mL for DPPH assay and EC50 = 0.52 ± 0.6 mg/mL for FRAP assay). The cytotoxic analysis showed significant viability towards RAW 264.7 cell lines. Treatment with ginger EO at a concentration of 100 μg/mL showed significant anti-inflammatory activity by the significant reduction of LPS-induced nitric oxide (NO) production to 31.35 %, in comparison with the LPS-treated group. Further, the EO components were screened in order to provide mechanistic insights into Xanthine oxidase (XO) and iNOS inhibition. The docking results showed that the volatile constituents of ginger EO have substantial binding affinity with the active site residues of the receptor. The simulation conducted for the top compounds- geranyl acetate and β-eudesmol having the highest binding affinity towards both the proteins XO and iNOS respectively. Both the results showed the compounds steadily interacted with both of the proteins active site residues throughout the simulation. The current analysis showed the phytoconstituents showed better antioxidant activity than the EO. Hence these compounds could be further considered for in vitro and in vivo evaluation for the development of efficient phytopharmaceuticals.
{"title":"Bioevaluation, pharmacokinetics and molecular docking study of terpenoid-rich rhizome essential oil of Zingiber officinale","authors":"Ananya Nayak, Ayushman Gadnayak, Chiranjibi Sahoo, Sudipta Jena, Asit Ray, Pratap Chandra Panda, Sanghamitra Nayak, Ambika Sahoo","doi":"10.1016/j.genrep.2024.102027","DOIUrl":"10.1016/j.genrep.2024.102027","url":null,"abstract":"<div><div>The study focuses on the phytochemical composition, <em>in vitro</em> and <em>in silico</em> antioxidant and NO reduction potential of <em>Zingiber Officinale</em> rhizome essential oil (ZOREO). The GC–MS analysis has revealed the presence of 47 volatile constituents representing 94.35 % of the total area of EO. The major compounds identified were α-zingiberene (20.58 %), geranial (10 %), β-sesquiphellandrene (7.39 %), and neral (7.23 %). The EO was rich in sesquiterpene hydrocarbons (45.93 %) followed by monoterpene hydrocarbons (29.29 %). The EO showed moderate antioxidant activity compared to the standard (IC<sub>50</sub> = 5.16 ± 0.4 mg/mL for DPPH assay and EC<sub>50</sub> = 0.52 ± 0.6 mg/mL for FRAP assay). The cytotoxic analysis showed significant viability towards RAW 264.7 cell lines. Treatment with ginger EO at a concentration of 100 μg/mL showed significant anti-inflammatory activity by the significant reduction of LPS-induced nitric oxide (NO) production to 31.35 %, in comparison with the LPS-treated group. Further, the EO components were screened in order to provide mechanistic insights into Xanthine oxidase (XO) and iNOS inhibition. The docking results showed that the volatile constituents of ginger EO have substantial binding affinity with the active site residues of the receptor. The simulation conducted for the top compounds- geranyl acetate and β-eudesmol having the highest binding affinity towards both the proteins XO and iNOS respectively. Both the results showed the compounds steadily interacted with both of the proteins active site residues throughout the simulation. The current analysis showed the phytoconstituents showed better antioxidant activity than the EO. Hence these compounds could be further considered for <em>in vitro</em> and <em>in vivo</em> evaluation for the development of efficient phytopharmaceuticals.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102027"},"PeriodicalIF":1.0,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vitamin D deficiency can negatively impact the health and training efficiency of athletes. The rs10741657(G) allele in CYP2R1 gene has been associated with lower vitamin D status, potentially contributing to vitamin D deficiency observed across various populations, including Indians. This study aimed to investigate whether the CYP2R1 (rs10741657) polymorphism is contributing to the vitamin D status among Indian athletes. Blood samples were collected from 92 Indian elite and sub-elite athletes participating in weightlifting (29), badminton (32), athletics (14), and rowing (17) for laboratory analysis. PCR-RFLP of the CYP2R1 (rs10741657) SNP was performed, and vitamin D levels were measured. Statistical analysis was done to assess the association between genotype and vitamin D status. Vitamin D levels were comparable between genders but differed across sports, with indoor athletes, especially badminton players having higher levels. The AA genotype was associated with higher vitamin D levels compared to the GG and AG genotypes. Vitamin D deficiency is highly prevalent among the athletes studied. This deficiency may be due to the higher prevalence of the alternate allele (G) of CYP2R1 polymorphism, suggesting that genetic predisposition plays a key role in determining the vitamin D status of athletes.
维生素 D 缺乏会对运动员的健康和训练效率产生负面影响。CYP2R1基因中的rs10741657(G)等位基因与较低的维生素D状态有关，可能是包括印度人在内的不同人群维生素D缺乏的原因之一。本研究旨在调查 CYP2R1（rs10741657）多态性是否与印度运动员的维生素 D 状态有关。研究人员采集了 92 名参加举重（29 人）、羽毛球（32 人）、田径（14 人）和赛艇（17 人）的印度精英和亚精英运动员的血样进行实验室分析。对 CYP2R1 (rs10741657) SNP 进行了 PCR-RFLP，并测量了维生素 D 水平。统计分析评估了基因型与维生素 D 状态之间的关联。不同性别之间的维生素 D 水平相当，但不同运动项目之间存在差异，室内运动员，尤其是羽毛球运动员的维生素 D 水平更高。与 GG 和 AG 基因型相比，AA 基因型的维生素 D 水平更高。在所研究的运动员中，维生素 D 缺乏症非常普遍。这种缺乏可能是由于 CYP2R1 多态性的另一等位基因（G）的流行率较高，这表明遗传易感性在决定运动员的维生素 D 状态方面起着关键作用。
{"title":"Genetic predisposition to vitamin D deficiency in Indian athletes: Role of CYP2R1 rs10741657 variant","authors":"Kavanashri N.M. , Keren Harish Tiwari , Sandeep Kumar Kotturu , Dinesh Yadav D.M. , Sudip Ghosh , Venkata Ramana Yagnambhatt","doi":"10.1016/j.genrep.2024.102033","DOIUrl":"10.1016/j.genrep.2024.102033","url":null,"abstract":"<div><div>Vitamin D deficiency can negatively impact the health and training efficiency of athletes. The rs10741657(G) allele in CYP2R1 gene has been associated with lower vitamin D status, potentially contributing to vitamin D deficiency observed across various populations, including Indians. This study aimed to investigate whether the CYP2R1 (rs10741657) polymorphism is contributing to the vitamin D status among Indian athletes. Blood samples were collected from 92 Indian elite and sub-elite athletes participating in weightlifting (29), badminton (32), athletics (14), and rowing (17) for laboratory analysis. PCR-RFLP of the CYP2R1 (rs10741657) SNP was performed, and vitamin D levels were measured. Statistical analysis was done to assess the association between genotype and vitamin D status. Vitamin D levels were comparable between genders but differed across sports, with indoor athletes, especially badminton players having higher levels. The AA genotype was associated with higher vitamin D levels compared to the GG and AG genotypes. Vitamin D deficiency is highly prevalent among the athletes studied. This deficiency may be due to the higher prevalence of the alternate allele (G) of CYP2R1 polymorphism, suggesting that genetic predisposition plays a key role in determining the vitamin D status of athletes.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102033"},"PeriodicalIF":1.0,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<div><div>The application of mesenchymal stem cells (MSCs), specifically those sourced from adipose tissue, has become a focal point in regenerative medicine, showing potential for effective cell therapy. Adipose-derived stem cells (ASCs) offer hope in treating various conditions, yet their viability and integration rates are hindered by the harsh post-transplantation environment marked by inflammation and oxidative stress at the injury site. <em>Lawsonia inermis</em> (henna), <em>Zizyphus spina-christ</em>i (Christ's Thorn), and <em>Glycyrrhiza glabra</em> (licorice) are three plants known for their potent antioxidant properties primarily due to their rich content of phenolic compounds and flavonoids. Their potential health benefits make them valuable in both traditional medicine and modern therapeutic applications.</div><div>This research delves into investigating and contrasting the effects of hydroalcoholic extracts from <em>Lawsonia inermis</em>, <em>Zizyphus spina-christi</em>, and <em>Glycyrrhiza glabra</em> on human adipose tissue stem cells as a pre-conditioning method to alleviate oxidative stress in cell therapy. ASCs were isolated from the pelvic cavity and abdomen, characterized using flow cytometry, and prompted to differentiate into adipogenic and osteogenic lineages. They were then subjected to different concentrations of the aforementioned herbal extracts at varying time frames, followed by MIC and MTT analysis. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and the Minimum Inhibitory Concentration (MIC) assay are both used to evaluate the effects of substances on cell viability and cell growth receptively. RNA extraction from ASCs and evaluation of antioxidant gene expression were conducted via Real-time PCR. Flow cytometry data confirmed the presence of MSC markers in the isolated cells from ASCs. Analysis of the herbal extract concentrations revealed that 50 ng/ml had a toxic effect on ASCs, establishing a toxicity reference point for adipose-derived MSCs. Real-time PCR results showcased a notable increase in the expression of antioxidant genes post-preconditioning with hydroalcoholic extracts. This study underscores, for the first time, the safety and lack of toxicity of preconditioning with these extracts for h.ASCs, enhancing their resilience and acclimatization under oxidative stress conditions, potentially boosting the therapeutic potential of h.ASCs. The antioxidant properties of <em>Lawsonia inermis</em>, <em>Zizyphus spina-christi</em>, and <em>Glycyrrhiza glabra</em> may enhance the expression of antioxidant genes (Sod, Gpx and Cata) through several mechanisms, including the activation of transcription factors like Nrf2 (Nuclear factor erythroid 2-related factor 2), which regulates antioxidant gene expression; modulation of signaling pathways such as MAPK (Mitogen-Activated Protein Kinase) and PI3K/Akt (Phosphoinositide 3-kinase/Protein Kinase B); and influencing epigenetic modifications
{"title":"Exploring herbal preconditioning strategies to improve adipose tissue stem cell therapy efficacy","authors":"Mahintaj Dara , Zeynab Zare-Moayedi , Yaghoob Taheri , Romina Tanideh , Shahrokh Zare , Farshid Kafilzadeh","doi":"10.1016/j.genrep.2024.102030","DOIUrl":"10.1016/j.genrep.2024.102030","url":null,"abstract":"<div><div>The application of mesenchymal stem cells (MSCs), specifically those sourced from adipose tissue, has become a focal point in regenerative medicine, showing potential for effective cell therapy. Adipose-derived stem cells (ASCs) offer hope in treating various conditions, yet their viability and integration rates are hindered by the harsh post-transplantation environment marked by inflammation and oxidative stress at the injury site. <em>Lawsonia inermis</em> (henna), <em>Zizyphus spina-christ</em>i (Christ's Thorn), and <em>Glycyrrhiza glabra</em> (licorice) are three plants known for their potent antioxidant properties primarily due to their rich content of phenolic compounds and flavonoids. Their potential health benefits make them valuable in both traditional medicine and modern therapeutic applications.</div><div>This research delves into investigating and contrasting the effects of hydroalcoholic extracts from <em>Lawsonia inermis</em>, <em>Zizyphus spina-christi</em>, and <em>Glycyrrhiza glabra</em> on human adipose tissue stem cells as a pre-conditioning method to alleviate oxidative stress in cell therapy. ASCs were isolated from the pelvic cavity and abdomen, characterized using flow cytometry, and prompted to differentiate into adipogenic and osteogenic lineages. They were then subjected to different concentrations of the aforementioned herbal extracts at varying time frames, followed by MIC and MTT analysis. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and the Minimum Inhibitory Concentration (MIC) assay are both used to evaluate the effects of substances on cell viability and cell growth receptively. RNA extraction from ASCs and evaluation of antioxidant gene expression were conducted via Real-time PCR. Flow cytometry data confirmed the presence of MSC markers in the isolated cells from ASCs. Analysis of the herbal extract concentrations revealed that 50 ng/ml had a toxic effect on ASCs, establishing a toxicity reference point for adipose-derived MSCs. Real-time PCR results showcased a notable increase in the expression of antioxidant genes post-preconditioning with hydroalcoholic extracts. This study underscores, for the first time, the safety and lack of toxicity of preconditioning with these extracts for h.ASCs, enhancing their resilience and acclimatization under oxidative stress conditions, potentially boosting the therapeutic potential of h.ASCs. The antioxidant properties of <em>Lawsonia inermis</em>, <em>Zizyphus spina-christi</em>, and <em>Glycyrrhiza glabra</em> may enhance the expression of antioxidant genes (Sod, Gpx and Cata) through several mechanisms, including the activation of transcription factors like Nrf2 (Nuclear factor erythroid 2-related factor 2), which regulates antioxidant gene expression; modulation of signaling pathways such as MAPK (Mitogen-Activated Protein Kinase) and PI3K/Akt (Phosphoinositide 3-kinase/Protein Kinase B); and influencing epigenetic modifications ","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102030"},"PeriodicalIF":1.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1016/j.genrep.2024.102032
Olajumoke Olufunmilayo Joseph , Samuel Olatunde Dahunsi , Anthony Okoh
Severe acute respiratory syndrome coronavirus – 2 (SARS-CoV-2) which was responsible for the COVID-19 pandemic has now been considered an endemic virus, that will continually produce sporadic outbreaks in different communities around the globe. Although, there are several surveillance studies on SARS-CoV-2 globally, including Nigeria, there is still an important need to understand the uniqueness of the strains of the virus that was circulating immediately after the pandemic, to add to the global database of information that will aid vaccine production and future preparedness against another SARS - related CoV pandemic.
Towards the end of the pandemic, between February – August 2022, SARS-CoV-2 was detected in a surveillance project in Ota – Ogun State Nigeria. We carried out a retrospective whole genome sequencing (WGS) analysis of SARS-CoV-2 in the previously reported positive sample, at Inqaba biotech in South Africa. RNA extraction was carried out using the Quick – RNA viral kit (Zymo) and library preparation was done by NEBNext ARTIC SARS-CoV-2 FS Library Prep kit (Illumina) according to the manufacturer's instruction. The WGS was carried out on the NextSeq500 platform by Illumina. The fastq file containing the unassembled raw sequence reads were submitted to NCBI SARS-CoV-2 resources, and a BioProject accession number PRJNA1076330 was issued. The DRAGEN Targeted Microbial, GISAID-CoVsurver mutation, BLAST and MAFFT applications were used for analysis.
The spike (s) gene of the study sequence possessed seven mutations (G142D, A163V, V213G, D614G, H655Y, N679K, P681H) and shared 99.4 % identity with those of the Wuhan reference sequence WIV04. The non-structural proteins (NSP) – 7,8,9,10 and 16 shared 100 % identity with bat/Yunnan/RaTG13 (a SARS- related CoV found in bats) sequence on GISAID. Although, the study sequence was obtained from an asymptomatic individual, the S mutations observed are known to be related to virulence, antigenic shift, enhanced transmissibility and host change. Thus, upon successful exploitation of this asymptomatic variant, it may possibly be transformed to a potential candidate for SARS-CoV-2 vaccine in future.
{"title":"A retrospective whole genome sequencing of SARS-CoV-2 isolate from respiratory sample of an individual from Ota – Ogun State, Nigeria","authors":"Olajumoke Olufunmilayo Joseph , Samuel Olatunde Dahunsi , Anthony Okoh","doi":"10.1016/j.genrep.2024.102032","DOIUrl":"10.1016/j.genrep.2024.102032","url":null,"abstract":"<div><p>Severe acute respiratory syndrome coronavirus – 2 (SARS-CoV-2) which was responsible for the COVID-19 pandemic has now been considered an endemic virus, that will continually produce sporadic outbreaks in different communities around the globe. Although, there are several surveillance studies on SARS-CoV-2 globally, including Nigeria, there is still an important need to understand the uniqueness of the strains of the virus that was circulating immediately after the pandemic, to add to the global database of information that will aid vaccine production and future preparedness against another SARS - related CoV pandemic.</p><p>Towards the end of the pandemic, between February – August 2022, SARS-CoV-2 was detected in a surveillance project in Ota – Ogun State Nigeria. We carried out a retrospective whole genome sequencing (WGS) analysis of SARS-CoV-2 in the previously reported positive sample, at Inqaba biotech in South Africa. RNA extraction was carried out using the Quick – RNA viral kit (Zymo) and library preparation was done by NEBNext ARTIC SARS-CoV-2 FS Library Prep kit (Illumina) according to the manufacturer's instruction. The WGS was carried out on the NextSeq500 platform by Illumina. The fastq file containing the unassembled raw sequence reads were submitted to NCBI SARS-CoV-2 resources, and a BioProject accession number PRJNA1076330 was issued. The DRAGEN Targeted Microbial, GISAID-CoVsurver mutation, BLAST and MAFFT applications were used for analysis.</p><p>The spike (s) gene of the study sequence possessed seven mutations (G142D, A163V, V213G, D614G, H655Y, N679K, P681H) and shared 99.4 % identity with those of the Wuhan reference sequence WIV04. The non-structural proteins (NSP) – 7,8,9,10 and 16 shared 100 % identity with bat/Yunnan/RaTG13 (a SARS- related CoV found in bats) sequence on GISAID. Although, the study sequence was obtained from an asymptomatic individual, the S mutations observed are known to be related to virulence, antigenic shift, enhanced transmissibility and host change. Thus, upon successful exploitation of this asymptomatic variant, it may possibly be transformed to a potential candidate for SARS-CoV-2 vaccine in future.</p></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102032"},"PeriodicalIF":1.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1016/j.genrep.2024.102028
Faiza Jamil , Kanwal Khan , Noor ul Ain Zahra , Reaz Uddin
Klebsiella pneumoniae (Kp) is a pathogenic bacterium known for its capacity to induce severe infections in humans, posing a significant threat to public health. Its resistance profile, particularly against carbapenem antibiotics, presents formidable challenges in clinical management. In response, a research endeavor was undertaken to discern prospective therapeutic targets against this pathogen. The investigation focused on delineating pivotal proteins involved in Host-Pathogen Interactions (HPIs) essential for the survival of Kp, thereby serving as potential targets for drug intervention. Through a careful screening process encompassing 438 proteins, 16 candidates were identified, prioritized based on criteria such as non-homology, essentiality, and druggability. Among these, 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (DapD), instrumental in lysine metabolism, emerged as a promising candidate for further scrutiny as a drug target against K. pneumoniae. Subsequently, employing virtual screening and molecular docking techniques, the study evaluated the 9214-compound FDA library to pinpoint potential drug candidates targeting the DapD protein. Ultimately, 15 compounds exhibiting promise were identified, suggesting the prospect of repurposing these agents for the treatment of Kp infections. This research delineates a promising step in the quest for novel therapeutics against K. pneumoniae, signifying a potential paradigm shift in combating this resilient bacterial strain. The findings hold promise for the development of more efficacious and safer antimicrobial agents, thereby addressing the pressing clinical need posed by antibiotic-resistant pathogens.
{"title":"Lysine metabolism pathway as a target for drug repurposing: In silico approach against carbapenem-resistant Klebsiella pneumoniae","authors":"Faiza Jamil , Kanwal Khan , Noor ul Ain Zahra , Reaz Uddin","doi":"10.1016/j.genrep.2024.102028","DOIUrl":"10.1016/j.genrep.2024.102028","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> (Kp) is a pathogenic bacterium known for its capacity to induce severe infections in humans, posing a significant threat to public health. Its resistance profile, particularly against carbapenem antibiotics, presents formidable challenges in clinical management. In response, a research endeavor was undertaken to discern prospective therapeutic targets against this pathogen. The investigation focused on delineating pivotal proteins involved in Host-Pathogen Interactions (HPIs) essential for the survival of Kp, thereby serving as potential targets for drug intervention. Through a careful screening process encompassing 438 proteins, 16 candidates were identified, prioritized based on criteria such as non-homology, essentiality, and druggability. Among these, 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate <em>N</em>-succinyltransferase (DapD), instrumental in lysine metabolism, emerged as a promising candidate for further scrutiny as a drug target against <em>K. pneumoniae</em>. Subsequently, employing virtual screening and molecular docking techniques, the study evaluated the 9214-compound FDA library to pinpoint potential drug candidates targeting the DapD protein. Ultimately, 15 compounds exhibiting promise were identified, suggesting the prospect of repurposing these agents for the treatment of Kp infections. This research delineates a promising step in the quest for novel therapeutics against <em>K. pneumoniae</em>, signifying a potential paradigm shift in combating this resilient bacterial strain. The findings hold promise for the development of more efficacious and safer antimicrobial agents, thereby addressing the pressing clinical need posed by antibiotic-resistant pathogens.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102028"},"PeriodicalIF":1.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1016/j.genrep.2024.102026
Hedia Zitouni , Vera Chayeb , Nozha Raguema , Marwa Ben Ali Gannoun , Touhami Mahjoub , Jean Guibourdenche , Wassim Y. Almawi
Background
The 11β-hydroxysteroid-dehydrogenase 1 (11β-HSD1) enzyme catalyzes the interconversion of cortisone and cortisol, with mainly oxoreductive activity in intact cells due to co-expression with hexose-6-phosphate dehydrogenase (H6PD). The uterine localization of 11β-HSD1 and its reduced placental expression in women with preeclampsia (PE) suggest a role for 11β-HSD1 in PE pathogenesis. We investigated the association of rs4844880 and rs846910 variants in the HSD11B1 gene with PE in Tunisian women.
Methods
The study cases comprised 334 women who presented with PE and 314 age-matched normotensive women who served as controls. The rs4844880 and rs846910 HSD11B1 gene variants were genotyped by real-time PCR.
Results
The rs4844880 T > A and rs846910 G > A minor allele frequencies were not different between PE cases and control women, which persisted after adjusting for age, BMI, gestational age, premature delivery, and baby weight. An association was noted for rs4844880 A/A genotype with a heightened risk of PE, which persisted after controlling key covariates. The (minor) A allele of rs4844880 was linked with elevated serum ALT and higher serum AST. In contrast, carriage of the rs846910 minor (A) allele was connected with higher baby weight on delivery and serum AST levels. Setting the major allele homozygotes (T-G) as a reference, a higher prevalence of double minor allele (A-A) haplotype was seen in PE cases than in corresponding controls, which persisted after controlling for age and BMI. Controlling for gestational age and baby weight identified the T-A haplotype and confirmed the association of the A-A haplotype with a heightened risk of PE.
Conclusion
Our results support an association between HSD11B1 polymorphisms and increased risk of PE and PE-associated clinical features.
背景11β-羟类固醇脱氢酶1（11β-HSD1）催化可的松和皮质醇的相互转化，由于与己糖-6-磷酸脱氢酶（H6PD）共同表达，在完整细胞中主要具有氧化还原活性。11β-HSD1 的子宫定位及其在子痫前期（PE）妇女胎盘中的表达减少表明，11β-HSD1 在子痫前期发病机制中发挥作用。我们研究了突尼斯妇女 HSD11B1 基因中 rs4844880 和 rs846910 变体与 PE 的关系。rs4844880和rs846910 HSD11B1基因变异通过实时PCR进行基因分型。结果rs4844880 T >A和rs846910 G >A小等位基因频率在PE病例和对照组妇女之间没有差异，在调整了年龄、体重指数、胎龄、早产和婴儿体重后，差异依然存在。rs4844880的A/A基因型与PE风险升高有关，在控制了关键的协变量后，这种关系依然存在。rs4844880 的（次要）A 等位基因与血清谷丙转氨酶升高和血清谷草转氨酶升高有关。相反，携带 rs846910 小（A）等位基因与婴儿出生时体重增加和血清 AST 水平升高有关。以主要等位基因的同源染色体（T-G）为参照，PE 病例中双次要等位基因（A-A）单倍型的发生率高于相应的对照组，在控制了年龄和体重指数后，这种情况依然存在。结论：我们的研究结果支持 HSD11B1 多态性与 PE 风险增加及 PE 相关临床特征之间存在关联。
{"title":"Relation of rs846910, rs4844880 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) polymorphisms with the risk of preeclampsia: A case-control study","authors":"Hedia Zitouni , Vera Chayeb , Nozha Raguema , Marwa Ben Ali Gannoun , Touhami Mahjoub , Jean Guibourdenche , Wassim Y. Almawi","doi":"10.1016/j.genrep.2024.102026","DOIUrl":"10.1016/j.genrep.2024.102026","url":null,"abstract":"<div><h3>Background</h3><p>The 11β-hydroxysteroid-dehydrogenase 1 (11β-HSD1) enzyme catalyzes the interconversion of cortisone and cortisol, with mainly oxoreductive activity in intact cells due to co-expression with hexose-6-phosphate dehydrogenase (H6PD). The uterine localization of 11β-HSD1 and its reduced placental expression in women with preeclampsia (PE) suggest a role for 11β-HSD1 in PE pathogenesis. We investigated the association of rs4844880 and rs846910 variants in the <em>HSD11B1</em> gene with PE in Tunisian women.</p></div><div><h3>Methods</h3><p>The study cases comprised 334 women who presented with PE and 314 age-matched normotensive women who served as controls. The rs4844880 and rs846910 <em>HSD11B1</em> gene variants were genotyped by real-time PCR.</p></div><div><h3>Results</h3><p>The rs4844880 T > A and rs846910 G > A minor allele frequencies were not different between PE cases and control women, which persisted after adjusting for age, BMI, gestational age, premature delivery, and baby weight. An association was noted for rs4844880 A/A genotype with a heightened risk of PE, which persisted after controlling key covariates. The (minor) A allele of rs4844880 was linked with elevated serum ALT and higher serum AST. In contrast, carriage of the rs846910 minor (A) allele was connected with higher baby weight on delivery and serum AST levels. Setting the major allele homozygotes (T-G) as a reference, a higher prevalence of double minor allele (A-A) haplotype was seen in PE cases than in corresponding controls, which persisted after controlling for age and BMI. Controlling for gestational age and baby weight identified the T-A haplotype and confirmed the association of the A-A haplotype with a heightened risk of PE.</p></div><div><h3>Conclusion</h3><p>Our results support an association between <em>HSD11B1</em> polymorphisms and increased risk of PE and PE-associated clinical features.</p></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102026"},"PeriodicalIF":1.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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