Pub Date : 2022-12-31DOI: 10.3126/hijost.v6i1.50599
S. R. Rai
The effect of herbal extracts of four ethnomedicinal herbs (Zanthoxylumarmatum, Litsea cubeba, Heracleum nepalense, and Evodia fraxinifolia) of culinary importance on the microbial quality of sukuti (a traditional dried meat product) was studied. Herbal extracts were prepared by grinding each herb to particle size < 250 μm, extracting in 50% (v/v) ethanol, and concentrating in a rotary vacuum evaporator at 50oC. Four of the spoilage and pathogenic microorganisms, viz., Salmonella, Staphylococcus, E. coli, and Lactobacillus were isolated from market sukuti samples and used as test organisms for the study. Herbal extracts at the concentration of 40, 20, 10, and 2 mg/ml were tested against the test organisms to determine the antimicrobial property of the extracts. The herbal extract showing the greatest antimicrobial activity was selected for use in optimized product (sukuti) development. The total phenolic content of the herbal extracts was also determined. The analyses were performed in triplicate. The data were checked for homogeneity before being analyzed with ANOVA in Genstat Release v12. The Fisher's Least Significant Difference (LSD) method was used to compare data means at a 5% level of significance. Zanthoxylum armatum at 40 mg/ml concentration showed the largest zone of inhibition (ZOI) against the test organisms and was therefore selected for final product development. Meat strips (1 cm × 1 cm × 25 cm) were marinated with Zanthoxylum armatum (40 mg/ml) extract at the rate of 2%, aged (24 h at 4 ± 2oC), and dried in a cabinet dryer for 2 days at 55oC. The total plate counts (TPCs) of control (untreated)- and herbal (treated) sukuti were carried out for 20 days at an interval of 10 days to determine the microbial stability of the final product. The TPC for the treated sample was significantly lower (p<0.05) than that of the untreated sample.
研究了四种具有烹饪价值的民族药材(花椒、山苍子、尼泊尔龙舌兰和吴茱萸)提取物对sukuti(传统肉干制品)微生物品质的影响。将各药材磨至粒径< 250 μm，用50% (v/v)乙醇提取，在50℃的旋转真空蒸发器中浓缩。四种腐败和致病微生物，即沙门氏菌、葡萄球菌、大肠杆菌和乳酸杆菌，从市场上的苏库提样品中分离出来，作为研究的试验生物。分别以40、20、10、2 mg/ml浓度的草药提取物对受试菌进行抑菌试验。筛选出抑菌活性最强的草药提取物用于优化产品(sukuti)的开发。测定了各草药提取物的总酚含量。分析一式三份。在Genstat Release v12中进行方差分析之前，检查数据的同质性。采用Fisher's Least Significant Difference (LSD)方法在5%显著性水平下比较数据均值。40 mg/ml浓度的花椒对试验生物的抑制区(ZOI)最大，因此被选中用于最终产品的开发。肉条(1 cm × 1 cm × 25 cm)用花椒(40 mg/ml)提取物以2%的比例腌制，4±20℃陈化24 h, 55℃柜式干燥机干燥2 d。对照(未处理)和草药(处理)苏库提的总平板计数(TPCs)每隔10天进行20天，以确定最终产品的微生物稳定性。处理后样品的TPC显著低于未处理样品(p<0.05)。
{"title":"Phytochemical Screening of Ethnomedicinal Herbal Extracts and their Effect on Microbial Quality of Sukuti","authors":"S. R. Rai","doi":"10.3126/hijost.v6i1.50599","DOIUrl":"https://doi.org/10.3126/hijost.v6i1.50599","url":null,"abstract":"The effect of herbal extracts of four ethnomedicinal herbs (Zanthoxylumarmatum, Litsea cubeba, Heracleum nepalense, and Evodia fraxinifolia) of culinary importance on the microbial quality of sukuti (a traditional dried meat product) was studied. Herbal extracts were prepared by grinding each herb to particle size < 250 μm, extracting in 50% (v/v) ethanol, and concentrating in a rotary vacuum evaporator at 50oC. Four of the spoilage and pathogenic microorganisms, viz., Salmonella, Staphylococcus, E. coli, and Lactobacillus were isolated from market sukuti samples and used as test organisms for the study. Herbal extracts at the concentration of 40, 20, 10, and 2 mg/ml were tested against the test organisms to determine the antimicrobial property of the extracts. The herbal extract showing the greatest antimicrobial activity was selected for use in optimized product (sukuti) development. The total phenolic content of the herbal extracts was also determined. The analyses were performed in triplicate. The data were checked for homogeneity before being analyzed with ANOVA in Genstat Release v12. The Fisher's Least Significant Difference (LSD) method was used to compare data means at a 5% level of significance. Zanthoxylum armatum at 40 mg/ml concentration showed the largest zone of inhibition (ZOI) against the test organisms and was therefore selected for final product development. Meat strips (1 cm × 1 cm × 25 cm) were marinated with Zanthoxylum armatum (40 mg/ml) extract at the rate of 2%, aged (24 h at 4 ± 2oC), and dried in a cabinet dryer for 2 days at 55oC. The total plate counts (TPCs) of control (untreated)- and herbal (treated) sukuti were carried out for 20 days at an interval of 10 days to determine the microbial stability of the final product. The TPC for the treated sample was significantly lower (p<0.05) than that of the untreated sample.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"122 7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81300354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/hijost.v6i1.50645
Peshal Pokharel, L. Shrestha
A thin-film transistor (TFT) is a special type of metal-oxide-semiconductor field-effect transistor (MOSFET) made by coating an insulating substrate with layers of an active semiconductor layer, metallic contacts, and the dielectric layer. FET transistors consist of three main components: source, gate, and drain. The main objective of the work is to fabricate the channel component by growing the ZnO nanostructure on the glass substrate using spin coating and spray pyrolysis methods. Thin films of zinc oxide (ZnO) were deposited on glass substrates by spin coating techniques from a precursor solution containing zinc acetate, ethanol and hydroxide of ammonia. After deposition, the films were centrifuged and evaporated. The application of spray pyrolysis has been used to deposit a wide variety of thin films, which are used in a variety of devices, such as solar cells, sensors and solid oxide fuel cells. It has been observed that the properties of the deposited thin films often depend on the preparation conditions; concentration levels of the precursor solution, coating time, electrical and optical properties of the glass substrate, etc. The average resistance of the sheet of samples F1, F5, F52, and F57 was 8.7 Ω, 9.14 Ω, 8.9 Ω and 9.42 Ω and of the samples, F2, F29, F39, and F53 were 9.5 Ω, 9.3 Ω, 9.9 Ω, 10.0 Ω respectively, at a growth temperature of 3400C. The thin films of ZnO were found to be highly transparent between the visible and near-infrared regions of the electromagnetic spectrum and the transmission of each sample decreases with three layers of ZnO seed layer. The decrease in the transmission of the samples confirms the coating of the ZnO seed layer on it. This work has demonstrated that transparent thin films can be fabricated using local techniques developed from locally available materials using less harmful chemical reagents such as zinc acetate. Such fabricated films are optically absorptive and inherently transmissive, further suggesting that they can be used as a channel material in thin film transistors.
{"title":"Fabrication of Transparent Thin Film for Application of Thin Film Transistor (TFT) and Microelectronics","authors":"Peshal Pokharel, L. Shrestha","doi":"10.3126/hijost.v6i1.50645","DOIUrl":"https://doi.org/10.3126/hijost.v6i1.50645","url":null,"abstract":"A thin-film transistor (TFT) is a special type of metal-oxide-semiconductor field-effect transistor (MOSFET) made by coating an insulating substrate with layers of an active semiconductor layer, metallic contacts, and the dielectric layer. FET transistors consist of three main components: source, gate, and drain. The main objective of the work is to fabricate the channel component by growing the ZnO nanostructure on the glass substrate using spin coating and spray pyrolysis methods. Thin films of zinc oxide (ZnO) were deposited on glass substrates by spin coating techniques from a precursor solution containing zinc acetate, ethanol and hydroxide of ammonia. After deposition, the films were centrifuged and evaporated. The application of spray pyrolysis has been used to deposit a wide variety of thin films, which are used in a variety of devices, such as solar cells, sensors and solid oxide fuel cells. It has been observed that the properties of the deposited thin films often depend on the preparation conditions; concentration levels of the precursor solution, coating time, electrical and optical properties of the glass substrate, etc. The average resistance of the sheet of samples F1, F5, F52, and F57 was 8.7 Ω, 9.14 Ω, 8.9 Ω and 9.42 Ω and of the samples, F2, F29, F39, and F53 were 9.5 Ω, 9.3 Ω, 9.9 Ω, 10.0 Ω respectively, at a growth temperature of 3400C. The thin films of ZnO were found to be highly transparent between the visible and near-infrared regions of the electromagnetic spectrum and the transmission of each sample decreases with three layers of ZnO seed layer. The decrease in the transmission of the samples confirms the coating of the ZnO seed layer on it. This work has demonstrated that transparent thin films can be fabricated using local techniques developed from locally available materials using less harmful chemical reagents such as zinc acetate. Such fabricated films are optically absorptive and inherently transmissive, further suggesting that they can be used as a channel material in thin film transistors.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"155 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81725263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/hijost.v6i1.50653
S. Gautam, Gyanu Thapa Magar, Mukesh Kumar Chhetri, Sujan Chaudhary
Community forest user groups (CFUGs) have been receiving different ecosystem services and facilities from the community forest, which are very crucial in their daily life activities. The present study is aimed at identifying the facilities and services received from the different plant species in the Janachana Community Forest, Rautahat district, Nepal. Altogether, 84 members of community forest groups participated in an interview using a semi-structured questionnaire. In total, 65 plants were identified in the study area for use as medicine, firewood, fodder, bedding, food, and other purposes. However, most plants were used as fodder for livestock. Altogether, fifty-eight plant species (89%) were used for multiple purposes (more than one use) and seven (11%) species were used for single purpose. Among the reported plant species, thirty species were used for medicinal purposes. Seeds and leaves were common parts of the plant used as medicine. Most of the plants were used to treat gastritis, cough, and blood clots, which seem to have high prevalence inthe study area. These results imply the various benefits taken by the CFUGs from the community forest in their daily life.
{"title":"Ethnobotanical study of Janachana community forest in Rautahat district, Nepal","authors":"S. Gautam, Gyanu Thapa Magar, Mukesh Kumar Chhetri, Sujan Chaudhary","doi":"10.3126/hijost.v6i1.50653","DOIUrl":"https://doi.org/10.3126/hijost.v6i1.50653","url":null,"abstract":"Community forest user groups (CFUGs) have been receiving different ecosystem services and facilities from the community forest, which are very crucial in their daily life activities. The present study is aimed at identifying the facilities and services received from the different plant species in the Janachana Community Forest, Rautahat district, Nepal. Altogether, 84 members of community forest groups participated in an interview using a semi-structured questionnaire. In total, 65 plants were identified in the study area for use as medicine, firewood, fodder, bedding, food, and other purposes. However, most plants were used as fodder for livestock. Altogether, fifty-eight plant species (89%) were used for multiple purposes (more than one use) and seven (11%) species were used for single purpose. Among the reported plant species, thirty species were used for medicinal purposes. Seeds and leaves were common parts of the plant used as medicine. Most of the plants were used to treat gastritis, cough, and blood clots, which seem to have high prevalence inthe study area. These results imply the various benefits taken by the CFUGs from the community forest in their daily life.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89442715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42079
Hem Shrestha, A. K. Jha, Saroj Nepal, Aatmaram Tiwari, Kamana Bantawa, Dhiren Subba Limbu
The Sky View Virtual Observatory was used for the systematic search of dust structures within the far-infrared loop G229-03. The source (object) responsible for the formation of the cavity of interest was detected by the Set of Identifications, Measurements, and Bibliography for Astronomical Data (SIMBAD) database. The total mass of the loop was 8.50031 × 1029 kg which is about 0.425 times the mass of the Sun at a distance of 1300 pc. The size of the cavity was 3.67° × 3.6°, whereas its core size was 0.531° × 0.255° located at R.A. (J2000) = 7h10m0.8s and Dec.(J2000) =15h55m30s. The minimum and maximum temperatures were between 20.24 ± 1.16 K and 18.63 ± 1.96 K respectively. In the core region, the average temperature was 19.53 K, approximately equal to Gaussian center 19.267 K with an offset temperature of 0.4 K showing that the core region of the cavity is dynamically stable. The Far-infrared loop was found to be located within a 1° radius around the high-velocity cloud HVC oriented by 45° to the plane of the sky. The inclination angle of the core of the loop was greater than 60° whereas the inclination angle for the larger structure was 13.71°. The Gaussian distribution of temperature was well fitted with the center of 19.267 K which shows that the cavity was in thermal equilibrium and the outer region with offset temperature of about 35 K suggesting that the loop was dynamically unstable possibly due to high-velocity cloud
{"title":"Dust Structure Nearby G229-03","authors":"Hem Shrestha, A. K. Jha, Saroj Nepal, Aatmaram Tiwari, Kamana Bantawa, Dhiren Subba Limbu","doi":"10.3126/hijost.v5i01.42079","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42079","url":null,"abstract":"The Sky View Virtual Observatory was used for the systematic search of dust structures within the far-infrared loop G229-03. The source (object) responsible for the formation of the cavity of interest was detected by the Set of Identifications, Measurements, and Bibliography for Astronomical Data (SIMBAD) database. The total mass of the loop was 8.50031 × 1029 kg which is about 0.425 times the mass of the Sun at a distance of 1300 pc. The size of the cavity was 3.67° × 3.6°, whereas its core size was 0.531° × 0.255° located at R.A. (J2000) = 7h10m0.8s and Dec.(J2000) =15h55m30s. The minimum and maximum temperatures were between 20.24 ± 1.16 K and 18.63 ± 1.96 K respectively. In the core region, the average temperature was 19.53 K, approximately equal to Gaussian center 19.267 K with an offset temperature of 0.4 K showing that the core region of the cavity is dynamically stable. The Far-infrared loop was found to be located within a 1° radius around the high-velocity cloud HVC oriented by 45° to the plane of the sky. The inclination angle of the core of the loop was greater than 60° whereas the inclination angle for the larger structure was 13.71°. The Gaussian distribution of temperature was well fitted with the center of 19.267 K which shows that the cavity was in thermal equilibrium and the outer region with offset temperature of about 35 K suggesting that the loop was dynamically unstable possibly due to high-velocity cloud","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76075257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42131
Babita Adhikari, Shonu Rai
Phytate and tannin are more pronounced antinutrients limiting the nutritional quality of fenugreek. The impact of roasting (130±5°C for 7 minutes), soaking (12 hrs. at room temperature) and germination (72 hrs. at 25°C) on bioactive compounds (polyphenol and antioxidant activity), nutritional and anti-nutritional compounds (phytate and tannin) in Fenugreek were studied. Experimental data were analyzed using the software GenStat 12th Edition. Protein increased significantly (p<0.05) during roasting, soaking, and germination whereas fat decreased significantly. Iron and calcium increased during roasting whereas decreased during soaking and germination. Maximum reduction of phytate (54.55%) and tannin (66.73%) were found when fenugreek seeds were germinated (72 hours). The reduction percentage of antinutrients by soaking (12 hours) and roasting (130±5⁰C for 7 mins) was found to be a lesser effective method compared to germination. All the treatments had a significant (p<0.05) impact on their bioactive components. Phenolic content increased significantly (p<0.05) during roasting, soaking, and germination. Antioxidant activity (IC50=1.28mg dm/ml) was found to be high in the germinated samples as compared to raw, roasted, and soaked samples. Hence, Germination of fenugreek seeds for 72 hours is concluded as the most effective and promising method for the reduction of antinutrients and increasing the nutritional components, phytochemicals, and antioxidant properties.
{"title":"Processing Impact on Nutritional, Antinutritional, and Phytochemical of Fenugreek Seeds (Trigonella Foenum-Graecum L.)","authors":"Babita Adhikari, Shonu Rai","doi":"10.3126/hijost.v5i01.42131","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42131","url":null,"abstract":"Phytate and tannin are more pronounced antinutrients limiting the nutritional quality of fenugreek. The impact of roasting (130±5°C for 7 minutes), soaking (12 hrs. at room temperature) and germination (72 hrs. at 25°C) on bioactive compounds (polyphenol and antioxidant activity), nutritional and anti-nutritional compounds (phytate and tannin) in Fenugreek were studied. Experimental data were analyzed using the software GenStat 12th Edition. Protein increased significantly (p<0.05) during roasting, soaking, and germination whereas fat decreased significantly. Iron and calcium increased during roasting whereas decreased during soaking and germination. Maximum reduction of phytate (54.55%) and tannin (66.73%) were found when fenugreek seeds were germinated (72 hours). The reduction percentage of antinutrients by soaking (12 hours) and roasting (130±5⁰C for 7 mins) was found to be a lesser effective method compared to germination. All the treatments had a significant (p<0.05) impact on their bioactive components. Phenolic content increased significantly (p<0.05) during roasting, soaking, and germination. Antioxidant activity (IC50=1.28mg dm/ml) was found to be high in the germinated samples as compared to raw, roasted, and soaked samples. Hence, Germination of fenugreek seeds for 72 hours is concluded as the most effective and promising method for the reduction of antinutrients and increasing the nutritional components, phytochemicals, and antioxidant properties.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72725650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42143
Anubhab Acharya, Mahalaxmi Pradhananga
The main aim was to study the effect of dry salting (0, 5, 10, 15, and 20% by weight) on the quality of lemon pickle. The samples were analyzed for microbial profile (Yeast, mold) and chemical characteristics at 15 days intervals till 90 days and the organoleptic quality of the product was evaluated after 90 days of storage. The data were statistically analyzed using two-way ANOVA (no blocking) at a 5% level of significance results showed that titratable acidity decreased with storage time till 60 days and remained constant thereafter at all levels of salt concentrations; Vitamin C remained fairly constant at all levels of salt concentrations over the storage periods whereas it decreased steadily during storage in the control sample (i.e. 0%); Mold count was nil at 15 days for all the level of salt except control and 5% concentration and Yeast count increased with storage time regardless of the salt% used in a lemon pickle. The optimized salt concentration for the preservation of lemon was found to be 15% salt which was the best product through sensory evaluation with the least microbial load and having appreciable sensory attributes and good vitamin C retention.
{"title":"Effect of Salt Concentration on the Preparation and Quality of Lemon Pickle (Nimki)","authors":"Anubhab Acharya, Mahalaxmi Pradhananga","doi":"10.3126/hijost.v5i01.42143","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42143","url":null,"abstract":"The main aim was to study the effect of dry salting (0, 5, 10, 15, and 20% by weight) on the quality of lemon pickle. The samples were analyzed for microbial profile (Yeast, mold) and chemical characteristics at 15 days intervals till 90 days and the organoleptic quality of the product was evaluated after 90 days of storage. The data were statistically analyzed using two-way ANOVA (no blocking) at a 5% level of significance results showed that titratable acidity decreased with storage time till 60 days and remained constant thereafter at all levels of salt concentrations; Vitamin C remained fairly constant at all levels of salt concentrations over the storage periods whereas it decreased steadily during storage in the control sample (i.e. 0%); Mold count was nil at 15 days for all the level of salt except control and 5% concentration and Yeast count increased with storage time regardless of the salt% used in a lemon pickle. The optimized salt concentration for the preservation of lemon was found to be 15% salt which was the best product through sensory evaluation with the least microbial load and having appreciable sensory attributes and good vitamin C retention.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88483108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42142
Bunty Maskey, A. Paudel, Nabindra Kumar Shrestha
The study aimed to determine the potential of oats and malted barley flours incorporation on cookie making. D-optimal mixture design was employed for formulating the recipe of cookies. Seven different formulations of recipe containing oats flour: malted barley flour: wheat flour in the ratio of 20:25:55, 15:30:55, 13:32:55, 10:35:55, 7:38:55, 5:40:55 and 100:0:0 was prepared. The prepared cookies were subjected to sensory evaluation for consumer acceptability and the data obtained were statistically analyzed using two-way ANOVA (no blocking) at a 5% level of significance. From the mean sensory scores, the formulation 10:35:55 was selected as the optimized formulation and subjected to proximate analysis. The optimized cookies were significantly (p<0.05) superior in the case of protein (7.50%), fat (22.93%), fiber (2.90%), and ash content (0.97%) to wheat cookies (6.43%, 18.83% 0.83% and 0.41% respectively) as seen higher in oats and malted barley flours than wheat flour. The calcium content was found to be significantly (p<0.05) higher in optimized cookies (35.3 mg/100g) than wheat cookies (18.40 mg/).
{"title":"Effect of Incorporation of Oats and Malted Barley Flours on the Quality of Cookies","authors":"Bunty Maskey, A. Paudel, Nabindra Kumar Shrestha","doi":"10.3126/hijost.v5i01.42142","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42142","url":null,"abstract":"The study aimed to determine the potential of oats and malted barley flours incorporation on cookie making. D-optimal mixture design was employed for formulating the recipe of cookies. Seven different formulations of recipe containing oats flour: malted barley flour: wheat flour in the ratio of 20:25:55, 15:30:55, 13:32:55, 10:35:55, 7:38:55, 5:40:55 and 100:0:0 was prepared. The prepared cookies were subjected to sensory evaluation for consumer acceptability and the data obtained were statistically analyzed using two-way ANOVA (no blocking) at a 5% level of significance. From the mean sensory scores, the formulation 10:35:55 was selected as the optimized formulation and subjected to proximate analysis. The optimized cookies were significantly (p<0.05) superior in the case of protein (7.50%), fat (22.93%), fiber (2.90%), and ash content (0.97%) to wheat cookies (6.43%, 18.83% 0.83% and 0.41% respectively) as seen higher in oats and malted barley flours than wheat flour. The calcium content was found to be significantly (p<0.05) higher in optimized cookies (35.3 mg/100g) than wheat cookies (18.40 mg/).","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76259150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42133
S. Khatri, Devraj Acharya
Cassava (Manihotesculentacrantz) is not true cereals are largely grown all over the world. They are very important due to their excellent nutritional contents especially protein, crude fiber, and carbohydrates. Biscuits were prepared by mixing cassava and wheat flour. Six treatments were prepared by adding 10%, 20%, 30%, 40%, and 50% cassava flour with wheat flour (Sample B, C, D, E, and F respectively) along with controlled treatment of 100% wheat flour (A). The proximate composition of flours was analyzed and prepared biscuits were subjected to sensory evaluation and shelf-life estimation. The obtained data was analyzed statistically by Genstat Discovery Edition 3 (DE3), for Analysis of Variance (ANOVA) at a 5% level of significance. Statistical analysis showed that sensory parameters and overall acceptability of 80 parts wheat and 20 parts cassava flour incorporated biscuit was significantly superior to all biscuits formulations. The proximate results of the best product showed that moisture content, crude fat, crude protein, crude fiber, total ash content, and total carbohydrate were found 3.44±0.25%, 13.80±0.33%, 8.13±0.13%, 1.29±0.19%, 2.41±0.06%, and 70.99±0.50 (g/100g) respectively. The shelf life of the best product was estimated by analyzing acid value and moisture content of the product by using three different packaging materials biaxially polypropylene (BOPP), high-density polypropylene (HDPE), and polypropylene (PP) respectively. The shelf life of the best product (80-20) wheat and cassava flours was found to be satisfactory for 8 weeks. The projected shelf life was found high in polypropylene (PP) and high-density polypropylene (HDPE) for 13.5 and 17.81 weeks for acid value and moisture content respectively.
{"title":"Quality Attributes and Shelf-Life Estimation of Cassava Flour Incorporated Bakery Product (Biscuit)","authors":"S. Khatri, Devraj Acharya","doi":"10.3126/hijost.v5i01.42133","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42133","url":null,"abstract":"Cassava (Manihotesculentacrantz) is not true cereals are largely grown all over the world. They are very important due to their excellent nutritional contents especially protein, crude fiber, and carbohydrates. Biscuits were prepared by mixing cassava and wheat flour. Six treatments were prepared by adding 10%, 20%, 30%, 40%, and 50% cassava flour with wheat flour (Sample B, C, D, E, and F respectively) along with controlled treatment of 100% wheat flour (A). The proximate composition of flours was analyzed and prepared biscuits were subjected to sensory evaluation and shelf-life estimation. The obtained data was analyzed statistically by Genstat Discovery Edition 3 (DE3), for Analysis of Variance (ANOVA) at a 5% level of significance. Statistical analysis showed that sensory parameters and overall acceptability of 80 parts wheat and 20 parts cassava flour incorporated biscuit was significantly superior to all biscuits formulations. The proximate results of the best product showed that moisture content, crude fat, crude protein, crude fiber, total ash content, and total carbohydrate were found 3.44±0.25%, 13.80±0.33%, 8.13±0.13%, 1.29±0.19%, 2.41±0.06%, and 70.99±0.50 (g/100g) respectively. The shelf life of the best product was estimated by analyzing acid value and moisture content of the product by using three different packaging materials biaxially polypropylene (BOPP), high-density polypropylene (HDPE), and polypropylene (PP) respectively. The shelf life of the best product (80-20) wheat and cassava flours was found to be satisfactory for 8 weeks. The projected shelf life was found high in polypropylene (PP) and high-density polypropylene (HDPE) for 13.5 and 17.81 weeks for acid value and moisture content respectively.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90706488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42134
Arjun Ghimire, Kishor Rai, H. Khanal
Intestinal parasitic infestation reflects a health threat with patients living with HIV & Tu-berculosis (TB). Parasitic infections caused by protozoa and helminths are the most com-mon infections worldwide. The present study was done to find out the frequency of intes-tinal parasitosis among HIV and Tuberculosis (TB) patients of Dharan total of 53 samples (>15 years) from HIV seropositive patients and 28 samples (>20years) from TB patients were collected in a clean, dry and capped fitted container and subjected to macroscopic and microscopic examination for ova, cyst, adult parasites and or segments of parasites. Stool samples were fixed in a 10% formalin-ether solution. Sedimentation technique, with modified acid-fast (Ziehl-Neelsen) staining method, was performed for opportunistic in-testinal parasites in both patients. Multi-parasitic infection was noted in the study. The overall prevalence of intestinal parasites was found to be 54 (66.67%). This result had shown that the participation of male patients was comparatively higher than female pa-tients. Out of 81 stool samples, 15 (27.78%) G. lamblia, 12(22.22%) E. histolytica, 12 (22.22%) Cryptosporidium parvum, 6 (11.11%), Isospora belli 4 (7.40%) Microspridium, 1(1.85%) Blastocystis hominis, 2(3.70%) and Taenia spp, 2(3.70%). To prevent this infec-tion appropriate health education should be given to the patients concerning disease trans-mission, antiparasitic therapy, personal hygiene, and safe drinking water.
{"title":"Intestinal Parasitosis among Human Immunodeficiency Virus and Tuberculosis Infected Patients from Dharan, Nepal","authors":"Arjun Ghimire, Kishor Rai, H. Khanal","doi":"10.3126/hijost.v5i01.42134","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42134","url":null,"abstract":" Intestinal parasitic infestation reflects a health threat with patients living with HIV & Tu-berculosis (TB). Parasitic infections caused by protozoa and helminths are the most com-mon infections worldwide. The present study was done to find out the frequency of intes-tinal parasitosis among HIV and Tuberculosis (TB) patients of Dharan total of 53 samples (>15 years) from HIV seropositive patients and 28 samples (>20years) from TB patients were collected in a clean, dry and capped fitted container and subjected to macroscopic and microscopic examination for ova, cyst, adult parasites and or segments of parasites. Stool samples were fixed in a 10% formalin-ether solution. Sedimentation technique, with modified acid-fast (Ziehl-Neelsen) staining method, was performed for opportunistic in-testinal parasites in both patients. Multi-parasitic infection was noted in the study. The overall prevalence of intestinal parasites was found to be 54 (66.67%). This result had shown that the participation of male patients was comparatively higher than female pa-tients. Out of 81 stool samples, 15 (27.78%) G. lamblia, 12(22.22%) E. histolytica, 12 (22.22%) Cryptosporidium parvum, 6 (11.11%), Isospora belli 4 (7.40%) Microspridium, 1(1.85%) Blastocystis hominis, 2(3.70%) and Taenia spp, 2(3.70%). To prevent this infec-tion appropriate health education should be given to the patients concerning disease trans-mission, antiparasitic therapy, personal hygiene, and safe drinking water.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87890135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.3126/hijost.v5i01.42129
Dhiren Subba Limbu, Samana Shrestha, Kamana Bantawa, R. Majhi, Milan Kharel
Intestinal parasitic infections have been a major public health burden of developing countries, especially in children. Higher prevalence has been reported among school children, mostly in rural areas of Nepal where water, toilets, hygiene, and sanitation facilities are insufficient. This cross-sectional study was carried out from April to September 2019 to determine the prevalence of intestinal parasitic infections among school-going children1-5 years of Dharan, Nepal, and to assess the associated risk factors. Stool samples were collected in a clean, dry, screw-capped, and wide-mouthed plastic container, kept in an icebox, and transported to the laboratory. Data relating to different risk factors were collected from the parents of 116 participants using a structured questionnaire. The parasites were identified by using the direct wet mount method and formal-ether concentration method. Pearson’s chi-square test was carried out to establish associations between dependent and independent variables using SPSS version 20, and the test considered a greater than < 0.05% as statistically significant with a 95% confidence level. Out of the 116 stool samples, 9 (7.75%) tested positive for the parasitic infections in which 5 (55.5%) were protozoa and 4 (44.45%) were helminths. The prevalent parasites, were Entamoeba histolytica (23%), Hyamenolepis nana (22%), Giardia lamblia (11%), Hookworm (11%), Entamoeba coli (11%), Ascaris lumbricoides (11%), and Intestinal parasitic infections had a significant association with drinking water, bowel syndrome, bathing habit, toilet facility, and washing hands after toilets (p<0.05). Lack of toilets, poor hygiene, and unsafe drinking water were the main risk factors. Improved hygienic practices, safe drinking water, and the use of latrines could lower the rate of parasitic infections.
肠道寄生虫感染一直是发展中国家，特别是儿童的主要公共卫生负担。据报告，学龄儿童的患病率较高，主要是在尼泊尔农村地区，那里的水、厕所、个人卫生和环境卫生设施不足。这项横断面研究于2019年4月至9月进行，旨在确定尼泊尔达兰1-5岁学龄儿童肠道寄生虫感染的患病率，并评估相关风险因素。粪便样本收集在干净、干燥、螺旋盖、宽口塑料容器中，保存在冰箱中，并运送到实验室。使用结构化问卷从116名参与者的父母那里收集了与不同风险因素相关的数据。采用直接湿贴法和甲醛浓度法对寄生虫进行鉴定。使用SPSS version 20进行Pearson卡方检验以建立因变量与自变量之间的关联，该检验认为大于< 0.05%具有统计学意义，置信水平为95%。116份粪便标本中，寄生虫感染阳性9份(7.75%)，其中原虫5份(55.5%)，蠕虫4份(44.45%)。常见寄生虫为溶组织内阿米巴原虫(23%)、小袋膜虫(22%)、兰氏贾第虫(11%)、钩虫(11%)、大肠内阿米巴原虫(11%)、类蚓蛔虫(11%)，肠道寄生虫感染与饮水、肠道综合征、洗澡习惯、厕所设施和如厕后洗手有显著相关性(p<0.05)。缺乏厕所、卫生条件差和饮用水不安全是主要的危险因素。改善卫生习惯、安全饮用水和使用厕所可以降低寄生虫感染率。
{"title":"Intestinal Parasitic Infections and Associated Risk Factors Among School-going Children of Age 1-5 years in Dharan, Eastern Nepal","authors":"Dhiren Subba Limbu, Samana Shrestha, Kamana Bantawa, R. Majhi, Milan Kharel","doi":"10.3126/hijost.v5i01.42129","DOIUrl":"https://doi.org/10.3126/hijost.v5i01.42129","url":null,"abstract":" Intestinal parasitic infections have been a major public health burden of developing countries, especially in children. Higher prevalence has been reported among school children, mostly in rural areas of Nepal where water, toilets, hygiene, and sanitation facilities are insufficient. This cross-sectional study was carried out from April to September 2019 to determine the prevalence of intestinal parasitic infections among school-going children1-5 years of Dharan, Nepal, and to assess the associated risk factors. Stool samples were collected in a clean, dry, screw-capped, and wide-mouthed plastic container, kept in an icebox, and transported to the laboratory. Data relating to different risk factors were collected from the parents of 116 participants using a structured questionnaire. The parasites were identified by using the direct wet mount method and formal-ether concentration method. Pearson’s chi-square test was carried out to establish associations between dependent and independent variables using SPSS version 20, and the test considered a greater than < 0.05% as statistically significant with a 95% confidence level. Out of the 116 stool samples, 9 (7.75%) tested positive for the parasitic infections in which 5 (55.5%) were protozoa and 4 (44.45%) were helminths. The prevalent parasites, were Entamoeba histolytica (23%), Hyamenolepis nana (22%), Giardia lamblia (11%), Hookworm (11%), Entamoeba coli (11%), Ascaris lumbricoides (11%), and Intestinal parasitic infections had a significant association with drinking water, bowel syndrome, bathing habit, toilet facility, and washing hands after toilets (p<0.05). Lack of toilets, poor hygiene, and unsafe drinking water were the main risk factors. Improved hygienic practices, safe drinking water, and the use of latrines could lower the rate of parasitic infections.","PeriodicalId":12935,"journal":{"name":"Himalayan Journal of Science and Technology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82478452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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