Heredity最新文献_第10页

The genetic basis of a colorful signal: the polymorphic dewlap of the slender anole (Anolis apletophallus) 一种彩色信号的遗传基础：细长的变色蛇（Anolis apletophallus）的多态脱壳。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-04-30 DOI: 10.1038/s41437-025-00763-z

Renata M. Pirani, Carlos F. Arias, John David Curlis, Daniel J. Nicholson, Jessica Stapley, W. Owen McMillan, Christian L. Cox, Michael L. Logan

Organisms often use colorful morphological traits to communicate with members of their own or other species. While “colorful signaling” systems exemplify well-known examples of the evolution of phenotypic diversity, the genetic basis of most of these traits remains unknown. Male lizards of the genus Anolis possess a colorful throat fan, or “dewlap”, that is flashed during social displays. These displays have been extensively studied in the context of their role in the adaptive radiation of the genus. In contrast, the genetic basis of the Anolis dewlap has received relatively little attention. Here, we studied the dewlap of the slender anole (Anolis apletophallus) which exhibits a dewlap polymorphism: males have either an entirely orange dewlap (“solid” morph) or a white dewlap with a basal orange spot (“bicolor” morph). To understand the inheritance of this polymorphism, we conducted 99 crosses between individuals from populations that were fixed for one morph (single/fixed/monomorphic) or contained both morphs (mixed/polymorphic). Next, we investigated the genetic architecture of this trait using a pooled population sequencing (Pool-seq) experiment. Our findings indicate that the slender anole dewlap polymorphism is best explained as an autosomal, single-locus, Mendelian trait with the solid morph allele dominant to the bicolor morph allele. Our outlier analysis of the Pool-seq data identified a region strongly associated with this trait and within this region we identified a promising candidate locus—the transcription factor single-minded 1 (SIM1)—that may underly the dewlap polymorphism.

生物经常使用丰富多彩的形态特征来与自己或其他物种的成员交流。虽然“彩色信号”系统是众所周知的表型多样性进化的例子，但大多数这些特征的遗传基础仍然未知。雄性蜥蜴属的蜥蜴有一个彩色的喉扇，或“dewlap”，在社会展示时闪烁。这些显示已被广泛研究的背景下，他们在适应辐射属的作用。相比之下，Anolis dewlap的遗传基础得到的关注相对较少。在这里，我们研究了细长的斑点斑马鱼（Anolis apletophallus）的斑马鱼，它表现出一种斑马鱼的多态性：雄性的斑马鱼要么有一个完全橙色的斑马鱼（“纯色”形态），要么有一个底部有橙色斑点的白色斑马鱼（“双色”形态）。为了了解这种多态性的遗传，我们在固定为一种形态（单一/固定/单态）或包含两种形态（混合/多态）的群体中的个体之间进行了99次杂交。接下来，我们使用池群体测序（Pool-seq）实验研究了该性状的遗传结构。我们的研究结果表明，细长的变色斑点多态可以最好地解释为常染色体的单位点孟德尔性状，其中固体型等位基因显性于双色型等位基因。我们对Pool-seq数据的异常值分析发现了一个与该性状密切相关的区域，并在该区域内发现了一个有希望的候选位点-转录因子单一思想1 (SIM1)-这可能是dewlap多态性的基础。

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引用次数: 0

Aneuploidy and ploidy variation conditioned by the B chromosome of maize. 玉米的非整倍性和倍性变异由B染色体决定。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-04-25 DOI: 10.1038/s41437-025-00764-y

Patrice S Albert, Hua Yang, Zhi Gao, Cassidy DeVore, James A Birchler

The supernumerary B chromosome of maize has a drive mechanism to maintain itself in a population despite being dispensible. This involves nondisjunction of the B centromere at the second pollen mitosis that produces the two sperm followed by preferential fertilization of the egg by the B containing sperm during double fertilization. During an introgression of the supernumerary B chromosome into the inbred line B73, an unusually high frequency of trisomies for A chromosomes was observed. Due to parallels to the High Loss phenomenon in which three or more B chromosomes in a specific genetic background cause chromosomal breakage at heterochromatic knob sites during the second pollen mitosis as well as ploidy changes, this phenomenon was revisited. Examination of pollen of the High Loss line revealed a high frequency of single sperm in the presence of the B chromosomes, which was previously not realized. Crosses to tetraploid females confirmed that the single sperm were diploid and functional but also revealed the presence of diploids with their A chromosomes derived solely from the tetraploid parent indicating a "diploid induction". Collectively, the results reveal two backgrounds in which the B drive mechanism is not confined to this chromosome causing detrimental effects by adherence of heterochromatic knobs and apparently A centromeres at the mitosis preceding sperm development. In most genetic backgrounds this process is restricted to the B chromosome but in B73 and the High Loss line, there is spillover to the normal chromosomes in distinct ways.

玉米的多余B染色体具有一种驱动机制，尽管在群体中是可有可无的，但仍能维持自身。这包括在产生两个精子的第二次花粉有丝分裂中B着丝粒的不分离，随后在双受精过程中含有B的精子优先使卵子受精。在多余的B染色体渗入自交系B73时，观察到A染色体三体的频率异常高。由于与高损失现象相似，在高损失现象中，具有特定遗传背景的三条或更多B染色体在第二次花粉有丝分裂期间导致异色球形位点的染色体断裂以及倍性变化，因此重新研究了这一现象。对高损失系花粉的检测发现，在B染色体存在的情况下，单精子的频率很高，这是以前没有意识到的。与四倍体雌性杂交证实了单精子是二倍体和功能，但也显示了二倍体的存在，其A染色体仅来自四倍体亲本，表明“二倍体诱导”。总的来说，结果揭示了两种背景，其中B驱动机制并不局限于这条染色体，在精子发育之前有丝分裂时，异色旋钮和明显的A着丝粒的粘附导致有害影响。在大多数遗传背景下，这一过程仅限于B染色体，但在B73和高损失系中，这一过程以不同的方式溢出到正常染色体。

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引用次数: 0

Does chromoanagenesis play a role in the origin of B chromosomes? 染色体再生在B染色体的起源中起作用吗？

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-04-19 DOI: 10.1038/s41437-025-00758-w

Andreas Houben, Jörg Fuchs, Ali Mohammad Banaei-Moghaddam, Jianyong Chen, Gihwan Kim, Taoran Liu

B chromosomes (Bs) exist in addition to the standard (A) chromosomes in a wide range of species. The process underlying their origin is still unclear. We propose pathways of intra- and interspecific origin of B chromosomes based on known mechanisms of chromosome evolution and available knowledge of their sequence composition in different species. We speculate that a mitotic or meiotic segregation error of one or more A chromosomes initiates, via chromoanagenesis, the formation of a proto-B chromosome. In the second step, proto-B chromosomes accumulate A chromosome- and organelle-derived sequences over time, most likely via DNA double-strand break (DSB) mis-repair. Consequently, the original structure of the early stage proto-B chromosomes becomes masked by continuous sequence incorporation. The similarity between A chromosome sequences integrated into B chromosomes and the original sequences on the donor chromosomes decreases over time if there is no selection pressure on these sequences on B chromosomes. However, besides chromoanagenesis, also other mechanisms leading to the formation of B chromosomes might exist.

除了标准(A)染色体外，B染色体(B)还存在于许多物种中。它们起源背后的过程尚不清楚。基于已知的染色体进化机制和对不同物种中B染色体序列组成的现有知识，我们提出了B染色体种内和种间起源的途径。我们推测，一条或多条a染色体的有丝分裂或减数分裂分离错误，通过染色体突变，启动了原b染色体的形成。在第二步，随着时间的推移，原b染色体积累了A染色体和细胞器衍生的序列，最有可能是通过DNA双链断裂（DSB）错误修复。因此，早期原b染色体的原始结构被连续的序列整合所掩盖。如果B染色体上的A染色体序列没有选择压力，那么与B染色体整合的A染色体序列与供体染色体上的原序列的相似性会随着时间的推移而降低。然而，除了染色体发育外，可能还存在其他导致B染色体形成的机制。

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引用次数: 0

Phylogeography of introgression: Spatial and temporal analyses identify two introgression events between brown and American black bears 进化演化的系统地理学：空间和时间分析确定了棕熊和美洲黑熊之间的两个进化演化事件。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-04-19 DOI: 10.1038/s41437-025-00762-0

Emily E. Puckett

Brown bears (Ursus arctos) colonized North America from Eurasia in two distinct and temporally separated waves. Once in North America they encountered endemic American black bears (U. americanus) during range expansions from eastern Beringia southwards into the interior of the continent. The establishment of sympatry between these species provided the opportunity for hybridization and introgression, which was previously identified at the species level using D-statistics. Both species have broad spatial ranges that should limit the extent of introgression, such that it is found primarily between sympatric populations. Here, we used range-wide sampling and whole genome sequencing of both bear species to test for spatial variability in introgression. We identified two pulses of introgression between brown and American black bears, and demonstrate the introgressed segments occur across spatially structured lineages in both species. The first pulse occurred 270–120 kya, near the initiation of intraspecific divergence, approximately 99–93 kya, within each species. This pulse occurred as sympatry was established in western North America. The second pulse occurred between western American black bears and North American brown bears and lasted to 9 kya. Introgression was bidirectional and sympatric lineages had more introgressed tracts and a larger proportion of the genome introgressed from the other species. This study advances our phylogeographic understanding of both iconic bear species through investigating the timing of divergence and gene flow as bears expanded and contracted their ranges across North America.

棕熊分两波从欧亚大陆移居北美，这两波在时间上是分开的。有一次在北美，它们从东白令陆桥向南扩展到大陆内部时遇到了特有的美洲黑熊（美国黑熊）。这些物种之间的同域关系的建立为杂交和渐渗提供了机会，这是以前在物种水平上使用D-statistics识别的。这两个物种都有广阔的空间范围，这应该限制了渗透的程度，因此它主要是在同域种群之间发现的。在这里，我们使用大范围采样和全基因组测序来测试这两种熊的空间变异。我们确定了棕熊和美洲黑熊之间的两个渐渗脉冲，并证明渐渗片段发生在两个物种的空间结构谱系中。第一次脉冲发生在270-120 kya，接近种内分化的开始，大约99-93 kya，在每个物种内。这种脉动发生在北美西部共情区建立的时候。第二次脉冲发生在美国西部黑熊和北美棕熊之间，持续了9个月。基因渐渗是双向的，同域谱系有更多的基因渐渗束和更大比例的基因组渐渗来自其他物种。这项研究通过调查熊在北美扩张和收缩活动范围时的分化时间和基因流动，推进了我们对这两个标志性熊物种的系统地理学理解。

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引用次数: 0

Recent habitat modification of a tropical dry forest hotspot drives population genetic divergence in the Mexican leaf frog: a landscape genetics approach 最近热带干旱森林热点的栖息地改变驱动墨西哥叶蛙种群遗传分化：景观遗传学方法

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-04-19 DOI: 10.1038/s41437-025-00761-1

Sara Covarrubias, Carla Gutiérrez-Rodríguez, Clementina González

Tropical dry forests (TDF) are among the ecosystems with the highest deforestation and transformation rates. Because of habitat loss and fragmentation, modified landscapes can impose resistance to the movement of individuals, with important genetic consequences. One of the most affected taxa due to habitat alteration are amphibians, which currently face extreme population declines globally. Here, we used single nucleotide polymorphisms (SNPs) to evaluate genetic diversity, genetic structure, and the effect of landscape elements on genetic connectivity of the Mexican tree frog (Agalychnis dacnicolor) in a TDF biodiversity hotspot in Mexico. We collected samples of 96 individuals from 16 sites located within fragmented areas of TDF and within continuous forest in the Chamela-Cuixmala region. Sampling sites from the fragmented forest showed slightly lower genetic diversity and effective population size compared to those in the continuous forest. We detected three admixed genetic groups, in which most of the sites within the fragmented forest were differentiated from the sites within continuous forest. Although these analyses suggest historical gene flow, we did not detect significant recent migration among the three genetic groups. While original vegetation (TDF + tropical evergreen forest), and in some areas, agriculture facilitated genetic connectivity, open-areas (grasslands + human settlements + exposed soil), and agriculture in other areas limited genetic connectivity in A. dacnicolor. This study helps to understand the factors shaping contemporary population divergence in highly modified complex landscapes, and highlights the importance to maintain connectivity in a rapidly changing ecosystem.

热带干林（TDF）是毁林率和转化率最高的生态系统之一。由于栖息地的丧失和破碎化，经过改造的景观可能对个体的迁移施加阻力，并产生重要的遗传后果。由于栖息地的改变，受影响最大的分类之一是两栖动物，它们目前在全球范围内面临着极端的数量下降。本研究利用单核苷酸多态性（snp）分析了墨西哥TDF生物多样性热点地区墨西哥树蛙（Agalychnis dacnicolor）的遗传多样性、遗传结构以及景观因素对其遗传连通性的影响。我们在Chamela-Cuixmala地区的TDF破碎区和连续森林内的16个地点收集了96个个体的样本。破碎林取样点的遗传多样性和有效种群规模略低于连续林取样点。我们发现了3个混合遗传群，其中破碎林内的大部分位点与连续林内的位点有分化。虽然这些分析表明了历史上的基因流动，但我们没有发现三个遗传群体之间的近期显著迁移。原始植被（TDF +热带常绿森林）和一些地区的农业促进了遗传连通性，而开放地区（草原+人类住区+暴露土壤）和其他地区的农业则限制了白毛杨的遗传连通性。本研究有助于理解在高度变化的复杂景观中形成当代人口差异的因素，并强调了在快速变化的生态系统中保持连通性的重要性。

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引用次数: 0

triangulaR: an R package for identifying AIMs and building triangle plots using SNP data from hybrid zones 三角形：一个R包，用于识别AIMs和建立三角图使用SNP数据从杂交区

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-04-12 DOI: 10.1038/s41437-025-00760-2

Ben J. Wiens, Lucas H. DeCicco, Jocelyn P. Colella

Hybridization provides a window into the speciation process and reshuffles parental alleles to produce novel recombinant genotypes. Presence or absence of specific hybrid classes across a hybrid zone can provide support for various modes of reproductive isolation. Early generation hybrid classes can be distinguished by their combination of hybrid index and interclass heterozygosity, which can be estimated with molecular data. Hybrid index and interclass heterozygosity are routinely calculated for studies of hybrid zones, but available resources for next-generation sequencing datasets are computationally demanding and tools for visualizing triangle plots are lacking. Here, we provide a resource for identifying ancestry-informative markers (AIMs) from single nucleotide polymorphism (SNP) datasets, calculating hybrid index and interclass heterozygosity, and visualizing the relationship as a triangle plot. Our methods are implemented in the R package triangulaR. We validate our methods on an empirical dataset and simulations of genetic data from a hybrid zone between two parental groups at low, medium, and high levels of divergence. triangulaR provides accurate and precise estimates of hybrid index and interclass heterozygosity with sample sizes as low as five individuals per parental group, and similar levels of error as another program for hybrid index and interclass heterozygosity estimation, bgchm. We explore various allele frequency difference thresholds for AIM identification, and how this threshold influences the accuracy and precision of hybrid index and interclass heterozygosity estimates. We contextualize interpretation of triangle plots by describing theoretical expectations under Hardy-Weinberg Equilibrium and provide recommendations for best practices for identifying AIMs and building triangle plots.

杂交为物种形成过程提供了一个窗口，并重组亲本等位基因以产生新的重组基因型。杂交区域中特定杂交类别的存在或缺失可以为各种繁殖隔离模式提供支持。通过杂交指数和种间杂合度的组合来区分早期杂种类别，并利用分子数据来估计杂种类别。杂交指数和类间杂合度是杂交区研究的常规计算方法，但下一代测序数据集的可用资源对计算要求很高，而且缺乏可视化三角图的工具。在这里，我们提供了从单核苷酸多态性（SNP）数据集中识别祖先信息标记（AIMs）的资源，计算杂交指数和类间杂合度，并将其关系可视化为三角形图。我们的方法是在R包三角形中实现的。我们在一个经验数据集上验证了我们的方法，并模拟了两个亲本群体之间低、中、高水平分化的杂交区遗传数据。三角模型提供了准确和精确的杂交指数和类间杂合度估计，每个亲本群体的样本量低至5个个体，其误差水平与另一个杂交指数和类间杂合度估计程序bgchm相似。我们探索了用于AIM鉴定的不同等位基因频率差异阈值，以及该阈值如何影响杂交指数和类间杂合度估计的准确性和精度。我们通过描述Hardy-Weinberg均衡下的理论期望，将三角图的解释背景化，并为确定目标和构建三角图提供最佳实践建议。

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引用次数: 0

EMIBD9: Estimating 9 condensed IBD coefficients, inbreeding and relatedness from marker genotypes EMIBD9：估计9个浓缩IBD系数，近交和标记基因型的相关性。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-03-27 DOI: 10.1038/s41437-024-00739-5

Jinliang Wang

EMIBD9 is a computer programme implementing two likelihood methods for estimating the 9 condensed IBD coefficients, Δ = {Δ1, Δ2, …, Δ9}, between a pair of individuals from their genotype data. Inbreeding coefficients of and relatedness (or kinship coefficient) between individuals are then calculated from the estimated Δ. One method is designed to apply to a small sample or a sample containing a high proportion of close relatives where allele frequencies and their powers or products are poorly estimated by assuming a large sample of non-inbred and unrelated individuals. It adopts an expectation maximisation (EM) algorithm to estimate both Δ and allele frequencies jointly and iteratively. The other method is designed to apply to a large sample of individuals containing few close relatives. It is fast because it, like all previous estimators, estimates Δ only and does not make iterative updates of allele frequencies by accounting for the inferred relatedness through the EM algorithm. EMIBD9 has both methods implemented for multiple computer platforms (Windows, Mac and Linux), and the Windows version has a GUI that facilitates data input and results visualisation. The GUI can also be used to simulate genotype data which are used to investigate factors affecting relatedness estimation accuracy, to optimise the experimental design of a relatedness study, and to compare the performance of different relatedness estimators.

EMIBD9是一个计算机程序，实现两种似然方法，用于估计一对个体之间基因型数据的9个浓缩IBD系数Δ = {Δ1， Δ2，…，Δ9}。然后根据估计的Δ计算个体之间的近交系数和亲缘关系系数（或亲属系数）。一种方法被设计用于小样本或含有高比例近亲的样本，在这些样本中，假设有大量非近亲繁殖和无亲缘关系的个体，就无法很好地估计等位基因频率及其功率或产物。采用期望最大化（EM）算法对Δ和等位基因频率进行联合迭代估计。另一种方法被设计用于包含很少近亲的大量个体样本。它是快速的，因为它像所有以前的估计器一样，只估计Δ，而不通过EM算法计算推断的相关性来对等位基因频率进行迭代更新。EMIBD9为多种计算机平台（Windows、Mac和Linux）实现了这两种方法，Windows版本有一个GUI，便于数据输入和结果可视化。GUI还可以用于模拟基因型数据，用于研究影响相关性估计精度的因素，优化相关性研究的实验设计，并比较不同相关性估计器的性能。

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引用次数: 0

Correction: Revealing stable SNPs and genomic prediction insights across environments enhance breeding strategies of productivity, defense, and climate-adaptability traits in white spruce 更正：揭示稳定的snp和跨环境的基因组预测见解增强了白云杉生产力、防御和气候适应性性状的育种策略。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-03-24 DOI: 10.1038/s41437-025-00757-x

Eduardo P. Cappa, Charles Chen, Jennifer G. Klutsch, Jaime Sebastian-Azcona, Blaise Ratcliffe, Xiaojing Wei, Letitia Da Ros, Yang Liu, Sudarshana Reddy Bhumireddy, Andy Benowicz, Shawn D. Mansfield, Nadir Erbilgin, Barb R. Thomas, Yousry A. El-Kassaby

引用次数: 0

Environmentally induced variation in sperm sRNAs is linked to gene expression and transposable elements in zebrafish offspring 环境诱导的精子sRNAs变异与斑马鱼后代的基因表达和转座因子有关。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-03-22 DOI: 10.1038/s41437-025-00752-2

Alice M. Godden, Willian T. A. F. Silva, Berrit Kiehl, Cécile Jolly, Leighton Folkes, Ghazal Alavioon, Simone Immler

Environmental factors affect not only paternal condition but may translate into the following generations where sperm-mediated small RNAs (sRNAs) can contribute to the transmission of paternal effects. sRNAs play a key role in the male germ line in genome maintenance and repair, and particularly in response to environmental stress and the resulting increase in transposable element (TE) activity. Here, we investigated how the social environment (high competition, low competition) of male zebrafish Danio rerio affects sRNAs in sperm and how these are linked to gene expression and TE activity in their offspring. In a first experiment, we collected sperm samples after exposing males to each social environment for 2 weeks to test for differentially expressed sperm micro- (miRNA) and piwi-interacting RNAs (piRNA). In a separate experiment, we performed in vitro fertilisations after one 2-week period using a split-clutch design to control for maternal effects and collected embryos at 24 h to test for differentially expressed genes and TEs. We developed new computational prediction tools to link sperm sRNAs with differentially expressed TEs and genes in the embryos. Our results support the idea that the molecular stress response in the male germ line has significant down-stream effects on the molecular pathways, and we provide a direct link between sRNAs, TEs and gene expression.

环境因素不仅影响父亲的状况，而且可能转化为后代，其中精子介导的小rna （sRNAs）可以促进父系效应的传播。sRNAs在雄性生殖系基因组的维持和修复中起着关键作用，特别是在对环境胁迫的反应和由此引起的转座因子（TE）活性的增加中。在这里，我们研究了雄性斑马鱼的社会环境（高竞争和低竞争）如何影响精子中的sRNAs，以及这些sRNAs如何与后代的基因表达和TE活性相关联。在第一个实验中，我们将雄性暴露于每种社会环境2周后收集精子样本，以检测精子微rna （miRNA）和piwi相互作用rna （piRNA）的差异表达。在另一项单独的实验中，我们在2周后使用分离离合器设计进行体外受精以控制母体效应，并在24小时收集胚胎以检测差异表达基因和TEs。我们开发了新的计算预测工具，将精子sRNAs与胚胎中差异表达的TEs和基因联系起来。我们的研究结果支持了雄性生殖系分子应激反应对分子通路有显著下游影响的观点，我们提供了sRNAs、TEs和基因表达之间的直接联系。

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引用次数: 0

Sperm DNA methylation landscape and its links to male fertility in a non-model teleost using EM-seq 精子DNA甲基化景观及其与非模型硬骨鱼男性生育能力的联系。

IF 3.1 2区生物学 Q2 ECOLOGY

Heredity

Pub Date : 2025-03-18 DOI: 10.1038/s41437-025-00756-y

Fotis Pappas, Martin Johnsson, Göran Andersson, Paul V. Debes, Christos Palaiokostas

Differential DNA methylation due to epigenetic phenomena is crucial in regulating gene expression. Understanding the consequences of such differential expression on sperm quality parameters may provide insights into the underlying mechanisms of male reproductive success. Nonetheless, male fertility in fish remains understudied despite its critical importance to overall reproductive success in nature and captivity. This study investigated the DNA methylation landscape in spermatozoa of domesticated Arctic charr (Salvelinus alpinus) and its associations with sperm quality parameters. Computer assisted-semen analysis (CASA) was performed in 47 sperm samples of farmed Arctic charr, followed by enzymatic methylation sequencing (EM-seq). Our results showed that the DNA of Arctic charr sperm is highly methylated (mean value of ~86%), though variations were observed in genomic features involved in gene regulation. Methylation at variable CpG sites exhibited regional correlation decaying by physical distance, while methylation similarities among individuals were strongly coupled with genetic variation and mirrored pedigree structure. Comethylation network analyses for promoters, CpG islands and first introns revealed genomic modules significantly correlated with sperm quality traits (p < 0.05; Bonferroni adjusted), with distinct patterns suggesting a resource trade-off between sperm concentration and kinematics. Furthermore, annotation and gene-set enrichment analysis highlighted biological mechanisms related to spermatogenesis, cytoskeletal regulation, and mitochondrial function, all vital to sperm physiology. These findings suggest that DNA methylation is a critical and fundamental factor influencing male fertility in Arctic charr, providing insights into the underlying mechanisms of male reproductive success.

表观遗传现象导致的差异DNA甲基化在基因表达调控中起着至关重要的作用。了解这种差异表达对精子质量参数的影响，可能有助于深入了解男性生殖成功的潜在机制。尽管如此，鱼类的雄性繁殖能力仍未得到充分研究，尽管它对自然和圈养环境下的整体繁殖成功至关重要。本研究研究了驯化北极鲑（Salvelinus alpinus）精子DNA甲基化景观及其与精子质量参数的关系。对养殖北极鲑的47个精子样本进行了计算机辅助精液分析（CASA），然后进行了酶甲基化测序（EM-seq）。我们的研究结果表明，北极charr精子的DNA高度甲基化（平均值约为86%），尽管在基因调控的基因组特征中观察到变异。不同CpG位点甲基化的区域相关性随物理距离而减弱，个体间甲基化相似性与遗传变异和反映的家系结构密切相关。启动子、CpG岛和第一内含子的基因组化网络分析显示，基因组模块与精子质量性状显著相关

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引用次数: 0