Pub Date : 2022-05-07DOI: 10.21608/jampr.2022.131451.1027
Ghada Gamal, Kamelia Abouelsaoud, E. Elekhnawy, Ghada Attia
Anabasis articulata (Forssk) Moq. is a rich bioactive member of Family Amaranthaceae. GC/MS analysis of Anabasis articulata methyl esters fraction notified twenty known fatty acids, including n -hexadecanoic acid, octadecanoic acid and tetradecanoic acid as major constituents. Chromatography analysis of successive fractions revealed the isolation of β -sitosterol 1 from its unsaponifiable fraction , caffeine 2 form its methylene chloride fraction , and 4-acetoxy phenol 3 from the ethyl acetate one. Structural elucidation of these isolates was performed by IR, EI-MS, 1 H NMR and 13 C NMR techniques. Compounds 2 and 3 were isolated for the first time from Anabasis articulata (Forssk) Moq. Meanwhile, methanol extract of the tested plant showed weak total phenolic content. Environmental adaptation of pathogenic microbes through formation of resistible biofilm is constructing a dramatic health hazard, which demanded the exploration of more microbial resisting treatments. One successful strategy is the use of Phytoextracts as noncytotoxic, microbial biofilm inhibitors. Adopting the broth microdilution method, Anabasis articulata (Forssk) Moq. methanol extract (AAME) showed antimicrobial activity against Pseudomonas aeruginosa clinical isolates at range of 32 to 256 µg/mL minimum inhibitory concentrations (MIC). The inhibiting efficiency of AAME against Pseudomonas aeruginosa biofilm formation was concluded at a concentration of half its MIC value using the crystal violet assay (CVA). Our results illustrate the ability of this extract to cutback the percentage of strong, and moderate biofilm forming P. aeruginosa clinical isolates from 41.18 % to 17.65 % and candidate it as futural antibiofilm agent.
{"title":"Anti-Biofilm Impact of Anabasis articulata (Forssk) Moq. Total Methanol Extract Against Pseudomonas aeruginosa Clinical Isolates","authors":"Ghada Gamal, Kamelia Abouelsaoud, E. Elekhnawy, Ghada Attia","doi":"10.21608/jampr.2022.131451.1027","DOIUrl":"https://doi.org/10.21608/jampr.2022.131451.1027","url":null,"abstract":"Anabasis articulata (Forssk) Moq. is a rich bioactive member of Family Amaranthaceae. GC/MS analysis of Anabasis articulata methyl esters fraction notified twenty known fatty acids, including n -hexadecanoic acid, octadecanoic acid and tetradecanoic acid as major constituents. Chromatography analysis of successive fractions revealed the isolation of β -sitosterol 1 from its unsaponifiable fraction , caffeine 2 form its methylene chloride fraction , and 4-acetoxy phenol 3 from the ethyl acetate one. Structural elucidation of these isolates was performed by IR, EI-MS, 1 H NMR and 13 C NMR techniques. Compounds 2 and 3 were isolated for the first time from Anabasis articulata (Forssk) Moq. Meanwhile, methanol extract of the tested plant showed weak total phenolic content. Environmental adaptation of pathogenic microbes through formation of resistible biofilm is constructing a dramatic health hazard, which demanded the exploration of more microbial resisting treatments. One successful strategy is the use of Phytoextracts as noncytotoxic, microbial biofilm inhibitors. Adopting the broth microdilution method, Anabasis articulata (Forssk) Moq. methanol extract (AAME) showed antimicrobial activity against Pseudomonas aeruginosa clinical isolates at range of 32 to 256 µg/mL minimum inhibitory concentrations (MIC). The inhibiting efficiency of AAME against Pseudomonas aeruginosa biofilm formation was concluded at a concentration of half its MIC value using the crystal violet assay (CVA). Our results illustrate the ability of this extract to cutback the percentage of strong, and moderate biofilm forming P. aeruginosa clinical isolates from 41.18 % to 17.65 % and candidate it as futural antibiofilm agent.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125675549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-10DOI: 10.21608/jampr.2022.125225.1023
A. Zayed, W. Negm, Amal M. Kabbash, S. Ezzat
{"title":"Marine-Derived Metabolites as Antimalarial Candidates Targeting Various Life Stages","authors":"A. Zayed, W. Negm, Amal M. Kabbash, S. Ezzat","doi":"10.21608/jampr.2022.125225.1023","DOIUrl":"https://doi.org/10.21608/jampr.2022.125225.1023","url":null,"abstract":"","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130053723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-29DOI: 10.21608/jampr.2022.125370.1024
M. Mabrouk, Sherin F Hammad, M. Elshahawy
{"title":"Thermodynamic and Kinetic Study of Chiral Separation of Some Non-Steroidal Anti-Inflammatory Drugs on Dinitrobenzamido Tetrahydrophenanthrene Stationary Phase","authors":"M. Mabrouk, Sherin F Hammad, M. Elshahawy","doi":"10.21608/jampr.2022.125370.1024","DOIUrl":"https://doi.org/10.21608/jampr.2022.125370.1024","url":null,"abstract":"","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115014434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-25DOI: 10.21608/jampr.2022.132917.1028
M. Mabrouk, Sherin F Hammad, Noha Elzawawy
{"title":"Different Spectrophotometric Methods of Operating Ratio Spectra for Analyzing Pharmaceutical Combinations of Loratadine and Dexamethasone","authors":"M. Mabrouk, Sherin F Hammad, Noha Elzawawy","doi":"10.21608/jampr.2022.132917.1028","DOIUrl":"https://doi.org/10.21608/jampr.2022.132917.1028","url":null,"abstract":"","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124646619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.21608/jampr.2021.98224.1020
A. Elsisi, S. Sokar, Sara R El-Mahrouk, Sally E Abu-Risha
Increased aerobic glycolysis in cancer, a phenomenon known as the Warburg effect, has been observed in various tumors and represents a major biochemical alteration associated with malignant transformation. Several cancers display an elevated expression of lactate dehydrogenase-A (LDHA), which is involved in tumor initiation, maintenance, and progression. Significantly, inhibition of LDH-A has been reported to have an antiproliferative effect on breast cancer and inhibited tumor progression. Accordingly, several LDH-A inhibitors are being tested for their anticancer activity, such as oxamate and galloflavin. In the current study, the anti-tumor activity of oxamate and galloflavin was tested in vitro using MCF7 and OVCAR-3 human carcinoma cell lines. Furthermore, both drugs were examined in combination with paclitaxel (Taxol). Additionally, the potential anti-tumor effect of oxamate in the solid Ehrlich carcinoma (SEC) mouse model was examined alone and in combination with paclitaxel. Oxamate and galloflavin significantly reduced cell survival of MCF7 and OVCAR3 cell lines. They also caused significant reductions in LDH enzyme activity and ATP cellular content in addition to a significant increase in MDA content. Both oxamate and galloflavin potentiated the anticancer effect of paclitaxel both in vivo and in vitro. Moreover, potentiation of apoptosis and the anti-angiogenic effect of paclitaxel by oxamate were found in vivo. In conclusion, LDH inhibitor may represent a promising agent that enhances the anti-tumor activity of paclitaxel chemotherapy.
{"title":"Potentiation of paclitaxel antitumor activity by galloflavin or oxamate as lactate dehydrogenase inhibitors","authors":"A. Elsisi, S. Sokar, Sara R El-Mahrouk, Sally E Abu-Risha","doi":"10.21608/jampr.2021.98224.1020","DOIUrl":"https://doi.org/10.21608/jampr.2021.98224.1020","url":null,"abstract":"Increased aerobic glycolysis in cancer, a phenomenon known as the Warburg effect, has been observed in various tumors and represents a major biochemical alteration associated with malignant transformation. Several cancers display an elevated expression of lactate dehydrogenase-A (LDHA), which is involved in tumor initiation, maintenance, and progression. Significantly, inhibition of LDH-A has been reported to have an antiproliferative effect on breast cancer and inhibited tumor progression. Accordingly, several LDH-A inhibitors are being tested for their anticancer activity, such as oxamate and galloflavin. In the current study, the anti-tumor activity of oxamate and galloflavin was tested in vitro using MCF7 and OVCAR-3 human carcinoma cell lines. Furthermore, both drugs were examined in combination with paclitaxel (Taxol). Additionally, the potential anti-tumor effect of oxamate in the solid Ehrlich carcinoma (SEC) mouse model was examined alone and in combination with paclitaxel. Oxamate and galloflavin significantly reduced cell survival of MCF7 and OVCAR3 cell lines. They also caused significant reductions in LDH enzyme activity and ATP cellular content in addition to a significant increase in MDA content. Both oxamate and galloflavin potentiated the anticancer effect of paclitaxel both in vivo and in vitro. Moreover, potentiation of apoptosis and the anti-angiogenic effect of paclitaxel by oxamate were found in vivo. In conclusion, LDH inhibitor may represent a promising agent that enhances the anti-tumor activity of paclitaxel chemotherapy.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124453067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-15DOI: 10.21608/jampr.2021.85883.1017
Samah F. El-Malla, Sherin F Hammad, S. Khashaba
{"title":"Quantitative Determination of Amlodipine and Valsartan in Pharmaceutical Dosage Form","authors":"Samah F. El-Malla, Sherin F Hammad, S. Khashaba","doi":"10.21608/jampr.2021.85883.1017","DOIUrl":"https://doi.org/10.21608/jampr.2021.85883.1017","url":null,"abstract":"","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127144793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-12DOI: 10.21608/jampr.2021.89542.1016
M. Mabrouk, Sherin F Hammad, Badiea Soliman, Amira H. Kamal
{"title":"Analysis of Counterfeit Sildenafil by Validated UV Spectrophotometric Methods","authors":"M. Mabrouk, Sherin F Hammad, Badiea Soliman, Amira H. Kamal","doi":"10.21608/jampr.2021.89542.1016","DOIUrl":"https://doi.org/10.21608/jampr.2021.89542.1016","url":null,"abstract":"","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122483412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-17DOI: 10.21608/JAMPR.2021.63273.1015
Nourhan M. Abdelbarey, M. Mabrouk, Miranda F. Kamal
Accurate, sensitive, robust and direct isocratic Reversed Phase-HPLC methods, using Evaporative Light Scattering Detector, (ELSD), had been developed and fully validated for the detection and quantification of two non-chromophoric nutraceuticals; Alpha Lipoic Acid (ALA) and Sucralose (SUC). Chromatographic separation was achieved using C18 Zoprax, 5 μm, 15 cm column for both compounds. For ALA, mobile phase was composed of acetonitrile: 0.1 M acetic acid (60:40, v/v), adjusted at pH 2.5, with 0.6 mL/min flow rate. For SUC, acetonitrile: deionized water (70:30, v/v) with 1 mL/min flow rate was used. Temperatures of drift tube, column, and spray chamber have been set to be 40°C and 30°C respectively during both assays. All varying chromatographic parameters were studied carefully. ALA and SUC had eluted at 4.81±0.02 min and 1.70±0.01 min, respectively. The obtained exponential ELSD responses, for the two developed methods, were linearly modeled using logarithmic transformation. Good Linearities had been achieved over the concentration ranges of 100-750 ppm and 16-500 ppm for ALA and SUC estimation respectively. The suggested methods disclosed excellent precision and accuracy levels. All validation parameters were fulfilled according to the USP elements and ICH guidelines. Satisfactory percentages of recovery (>97%) resulted upon methods application for the assay of each dietary supplement in its pharmaceutical formulation. Comparative statistical study was conducted between the proposed HPLC/ELSD methods and the reported HPLC/UV method for ALA and reported HPTLC method for SUC. Student’s t-tests and F-variance ratios for both methods had resulted in satisfactory values.
{"title":"HPLC/ELSD DETERMINATION AND VALIDATION OF ALPHA LIPOIC ACID AND SUCRALOSE IN BULK AND IN THEIR PHARMACEUTICAL DOSAGE FORMS","authors":"Nourhan M. Abdelbarey, M. Mabrouk, Miranda F. Kamal","doi":"10.21608/JAMPR.2021.63273.1015","DOIUrl":"https://doi.org/10.21608/JAMPR.2021.63273.1015","url":null,"abstract":"Accurate, sensitive, robust and direct isocratic Reversed Phase-HPLC methods, using Evaporative Light Scattering Detector, (ELSD), had been developed and fully validated for the detection and quantification of two non-chromophoric nutraceuticals; Alpha Lipoic Acid (ALA) and Sucralose (SUC). Chromatographic separation was achieved using C18 Zoprax, 5 μm, 15 cm column for both compounds. For ALA, mobile phase was composed of acetonitrile: 0.1 M acetic acid (60:40, v/v), adjusted at pH 2.5, with 0.6 mL/min flow rate. For SUC, acetonitrile: deionized water (70:30, v/v) with 1 mL/min flow rate was used. Temperatures of drift tube, column, and spray chamber have been set to be 40°C and 30°C respectively during both assays. All varying chromatographic parameters were studied carefully. ALA and SUC had eluted at 4.81±0.02 min and 1.70±0.01 min, respectively. The obtained exponential ELSD responses, for the two developed methods, were linearly modeled using logarithmic transformation. Good Linearities had been achieved over the concentration ranges of 100-750 ppm and 16-500 ppm for ALA and SUC estimation respectively. The suggested methods disclosed excellent precision and accuracy levels. All validation parameters were fulfilled according to the USP elements and ICH guidelines. Satisfactory percentages of recovery (>97%) resulted upon methods application for the assay of each dietary supplement in its pharmaceutical formulation. Comparative statistical study was conducted between the proposed HPLC/ELSD methods and the reported HPLC/UV method for ALA and reported HPTLC method for SUC. Student’s t-tests and F-variance ratios for both methods had resulted in satisfactory values.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115876359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/jampr.2020.23512.1002
W. Negm, K. A. El-Seoud, A. Kabbash, Mona El-Aasr
People are known to utilize plants for treatment of many diseases. Cycadales are one of these ancient plants that contained different active constituents. Due to the lack of adequate research on Cycadales, we conducted this study. The current study investigated and compared different biological activities for four different plants. Cycas thouarsii, Cycas pectinata, Dioon spinulosum and Encephalartos laurentianus were tested for the first time for cytotoxic, antioxidant and antimicrobial activities. Dioon spinulosum displayed significant cytotoxic activity on VERO cell line that was higher than 5-Fluorouracil. D. spinulosum possessed the highest radical scavenging activity followed by Cycas thouarsii. D. spinulosum showed the highest protective activity against DNA damage which was higher than the positive control. D. spinulosum showed the highest antihemolytic antioxidant activity followed by Encephalartos laurentianus. D. spinulosum presented the highest antimicrobial activity among the tested plants. Most Cycadales tested plants especially D. spinulosum displayed significant biological activities that are promising for future drug discovery.
{"title":"Biological Activity Investigation of Some Gymnosperm Plants Belong to Cycadales Order","authors":"W. Negm, K. A. El-Seoud, A. Kabbash, Mona El-Aasr","doi":"10.21608/jampr.2020.23512.1002","DOIUrl":"https://doi.org/10.21608/jampr.2020.23512.1002","url":null,"abstract":"People are known to utilize plants for treatment of many diseases. Cycadales are one of these ancient plants that contained different active constituents. Due to the lack of adequate research on Cycadales, we conducted this study. The current study investigated and compared different biological activities for four different plants. Cycas thouarsii, Cycas pectinata, Dioon spinulosum and Encephalartos laurentianus were tested for the first time for cytotoxic, antioxidant and antimicrobial activities. Dioon spinulosum displayed significant cytotoxic activity on VERO cell line that was higher than 5-Fluorouracil. D. spinulosum possessed the highest radical scavenging activity followed by Cycas thouarsii. D. spinulosum showed the highest protective activity against DNA damage which was higher than the positive control. D. spinulosum showed the highest antihemolytic antioxidant activity followed by Encephalartos laurentianus. D. spinulosum presented the highest antimicrobial activity among the tested plants. Most Cycadales tested plants especially D. spinulosum displayed significant biological activities that are promising for future drug discovery.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124821761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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