Pub Date : 1900-01-01DOI: 10.29296/2618723x-2022-01-06
E.V. Vesnina, M. Akimova, T. Barmina
{"title":"Features of the formation of diets nd feeding regimen of common igrunka in the conditions of a research laboratory","authors":"E.V. Vesnina, M. Akimova, T. Barmina","doi":"10.29296/2618723x-2022-01-06","DOIUrl":"https://doi.org/10.29296/2618723x-2022-01-06","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129510410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-04-08
Y. Gushchin, E. Belyaeva, J.Yu. Ustenko
{"title":"Dynamics of postmortem changes in carcasses of dead laboratory animals","authors":"Y. Gushchin, E. Belyaeva, J.Yu. Ustenko","doi":"10.29296/2618723x-2021-04-08","DOIUrl":"https://doi.org/10.29296/2618723x-2021-04-08","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130548347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2020-03-02
M. Miroshnikov, M. Makarova
Резюме. На сегодняшний день в распоряжении ученых для проведения токсикологических, фармакокинетических и других исследований, помимо грызунов, находится более десятка других лабораторных животных. Свиней уже давно используют для изучения трансплантации органов и тканей, хирургической подготовки студентов-медиков, однако их применение может быть значительно шире. Анатомическое сходство с человеком и крупные размеры этих животных позволяют успешно проводить исследования сердечно-сосудистой, пищеварительной и мочевыделительной систем, а также использовать в доклинических и токсикологических исследованиях. В связи с этим установление референсных интервалов биохимических показателей крови лабораторных свиней является актуальной задачей. В статье представлены референсные интервалы биохимических показателей крови карликовых свиней, полученные с применением классического подхода. Были использованы результаты биохимического анализа крови 15 самцов и 15 самок клинически здоровых карликовых свиней в возрасте 9–11 мес массой тела 14–26 кг, выращенных в нашем питомнике. Помимо основных биохимических показателей (креатинин, мочевина, аланинаминотрансфераза, аспартатаминотрансфераза, щелочная фосфатаза, холестерин, триглицериды, общий белок, альбумин, глюкоза и общий билирубин), представлены дополнительные параметры – расширенный липидный профиль, концентрация кальция и фосфора, активность ряда ферментов (лактатдегидрогеназа, креатинкиназа, амилаза, гамма-глутамилтрансфераза и холинэстераза), являющихся биохимическими маркерами повреждения различных систем и органов. Наиболее выраженные отличия обнаружены при исследовании липидного профиля самцов и самок карликовых свиней: концентрация общего холестерина и триглицеридов в крови самок была выше в среднем в 1,5 и 2 раза соответственно. Кроме того, в сыворотке крови самок концентрация холестерина липопротеинов низкой плотности была в среднем в 1,8 раз выше, чем в сыворотке крови самцов. Менее выраженные, но статистически значимые различия были обнаружены в отношении концентрации общего белка и глобулинов. Проведенный анализ представленных референсных интервалов с данными литературы показал сопоставимость полученных значений. Несмотря на значительное сходство биохимических профилей человека и лабораторных свиней, необходимо учитывать наличие видовых различий при рассмотрении результатов доклинических исследований.
{"title":"Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 3: mini pigs","authors":"M. Miroshnikov, M. Makarova","doi":"10.29296/2618723x-2020-03-02","DOIUrl":"https://doi.org/10.29296/2618723x-2020-03-02","url":null,"abstract":"Резюме. На сегодняшний день в распоряжении ученых для проведения токсикологических, фармакокинетических и других исследований, помимо грызунов, находится более десятка других лабораторных животных. Свиней уже давно используют для изучения трансплантации органов и тканей, хирургической подготовки студентов-медиков, однако их применение может быть значительно шире. Анатомическое сходство с человеком и крупные размеры этих животных позволяют успешно проводить исследования сердечно-сосудистой, пищеварительной и мочевыделительной систем, а также использовать в доклинических и токсикологических исследованиях. В связи с этим установление референсных интервалов биохимических показателей крови лабораторных свиней является актуальной задачей. В статье представлены референсные интервалы биохимических показателей крови карликовых свиней, полученные с применением классического подхода. Были использованы результаты биохимического анализа крови 15 самцов и 15 самок клинически здоровых карликовых свиней в возрасте 9–11 мес массой тела 14–26 кг, выращенных в нашем питомнике. Помимо основных биохимических показателей (креатинин, мочевина, аланинаминотрансфераза, аспартатаминотрансфераза, щелочная фосфатаза, холестерин, триглицериды, общий белок, альбумин, глюкоза и общий билирубин), представлены дополнительные параметры – расширенный липидный профиль, концентрация кальция и фосфора, активность ряда ферментов (лактатдегидрогеназа, креатинкиназа, амилаза, гамма-глутамилтрансфераза и холинэстераза), являющихся биохимическими маркерами повреждения различных систем и органов. Наиболее выраженные отличия обнаружены при исследовании липидного профиля самцов и самок карликовых свиней: концентрация общего холестерина и триглицеридов в крови самок была выше в среднем в 1,5 и 2 раза соответственно. Кроме того, в сыворотке крови самок концентрация холестерина липопротеинов низкой плотности была в среднем в 1,8 раз выше, чем в сыворотке крови самцов. Менее выраженные, но статистически значимые различия были обнаружены в отношении концентрации общего белка и глобулинов. Проведенный анализ представленных референсных интервалов с данными литературы показал сопоставимость полученных значений. Несмотря на значительное сходство биохимических профилей человека и лабораторных свиней, необходимо учитывать наличие видовых различий при рассмотрении результатов доклинических исследований.","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"281 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127391450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-04-06
M. Akimova, D. Akimov, T. Barmina, E.V. Vesnina, S.S. Dobrianskaya
{"title":"Influence of temperature factors on reproduction of laboratory mice and gerbils","authors":"M. Akimova, D. Akimov, T. Barmina, E.V. Vesnina, S.S. Dobrianskaya","doi":"10.29296/2618723x-2021-04-06","DOIUrl":"https://doi.org/10.29296/2618723x-2021-04-06","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133985978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-02-05
M. Akimova, M. Makarova, D. Akimov
{"title":"Evaluation of nesting behavior as an indicator of the level of distress in laboratory mice","authors":"M. Akimova, M. Makarova, D. Akimov","doi":"10.29296/2618723x-2021-02-05","DOIUrl":"https://doi.org/10.29296/2618723x-2021-02-05","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131374785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2020-03-06
S. Smirnov, A. A. Karpov, A. Gutsalova, I. Kurzina, D. Lytkina, E. Shchepkina, G. A. Plisko, V. Karev, A. Ivkina
Summary. In this study, the biocompatibility of composite materials based on hydroxyapatite and the copolymer lactide and glycolide with different ratios of components was studied on mice: composite material No. 1 – 95: 5 wt%, composite material No. 2 – 93: 7 wt%. During the experiments, 30 outbred male mice weighing 30–40 g were used, which before the operation were randomly divided into six groups depending on what material was implanted into the body: hydroxyapatite, lactide-glycolide copolymer, composite material No. 1, composite material no. 2; in the group of sham-operated animals, access to the pectoralis major muscle was performed without placing the material. Operations were performed to introduce the tested materials for the large pectoral muscle. The experi-ment lasted 22 days. On the 21st day after the operation, blood was collected from the retroorbital sinus to determine the level of white blood cells. After euthanasia in a CO2 box, macroscopic and histological examination of the pectoralis major muscle was performed. Staining of histological preparations was performed using hematoxylin-eosin and van gieson staining on connective tissue. The follow-ing parameters were evaluated: inflammation, fibrosis, and vascularization. It has been shown that isolated use of hydroxyapatite is accompanied by moderate fibrosis and local inflammation. In response to the implantation of a pure copolymer of lactide and glycolide, no reaction was observed on the part of the body. Among composite materials from the point of view of biocompatibility, the optimal material for implantation among the studied was a composite material with a ratio of hydroxyapatite to the copolymer of lactide and glycolide equal to 93:7 wt.% (composite material # 2), the use of which is characterized by a lower degree of inflammation and fibrosis in the implantation zone compared to hydroxyapatite. The greater biocompatibility of composite material No. 2 in comparison with hydroxyapatite and composite material No. 1 can probably be associated with an increase in the content of the copolymer of lactide and glycolide in its composition.
{"title":"Research of the biocompatibility of composite materials based on hydroxyapatitis and copolymer lactide-glycolide on laboratory mice Laboratory","authors":"S. Smirnov, A. A. Karpov, A. Gutsalova, I. Kurzina, D. Lytkina, E. Shchepkina, G. A. Plisko, V. Karev, A. Ivkina","doi":"10.29296/2618723x-2020-03-06","DOIUrl":"https://doi.org/10.29296/2618723x-2020-03-06","url":null,"abstract":"Summary. In this study, the biocompatibility of composite materials based on hydroxyapatite and the copolymer lactide and glycolide with different ratios of components was studied on mice: composite material No. 1 – 95: 5 wt%, composite material No. 2 – 93: 7 wt%. During the experiments, 30 outbred male mice weighing 30–40 g were used, which before the operation were randomly divided into six groups depending on what material was implanted into the body: hydroxyapatite, lactide-glycolide copolymer, composite material No. 1, composite material no. 2; in the group of sham-operated animals, access to the pectoralis major muscle was performed without placing the material. Operations were performed to introduce the tested materials for the large pectoral muscle. The experi-ment lasted 22 days. On the 21st day after the operation, blood was collected from the retroorbital sinus to determine the level of white blood cells. After euthanasia in a CO2 box, macroscopic and histological examination of the pectoralis major muscle was performed. Staining of histological preparations was performed using hematoxylin-eosin and van gieson staining on connective tissue. The follow-ing parameters were evaluated: inflammation, fibrosis, and vascularization. It has been shown that isolated use of hydroxyapatite is accompanied by moderate fibrosis and local inflammation. In response to the implantation of a pure copolymer of lactide and glycolide, no reaction was observed on the part of the body. Among composite materials from the point of view of biocompatibility, the optimal material for implantation among the studied was a composite material with a ratio of hydroxyapatite to the copolymer of lactide and glycolide equal to 93:7 wt.% (composite material # 2), the use of which is characterized by a lower degree of inflammation and fibrosis in the implantation zone compared to hydroxyapatite. The greater biocompatibility of composite material No. 2 in comparison with hydroxyapatite and composite material No. 1 can probably be associated with an increase in the content of the copolymer of lactide and glycolide in its composition.","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122624832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-02-03
A. Afanaseva, V. Saparova, T. A. Selmenskikh, I. E. Makarenko
{"title":"Optimal choice method of detection of the viability of cell cultures for tests on proliferation and cytotoxicity","authors":"A. Afanaseva, V. Saparova, T. A. Selmenskikh, I. E. Makarenko","doi":"10.29296/2618723x-2021-02-03","DOIUrl":"https://doi.org/10.29296/2618723x-2021-02-03","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"134 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128615690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.57034/2618723x-2022-04-01
D. Bylinskaya, M. Shchipakin, D. Vasiliev
{"title":"Branches of the aortic arch of the rat","authors":"D. Bylinskaya, M. Shchipakin, D. Vasiliev","doi":"10.57034/2618723x-2022-04-01","DOIUrl":"https://doi.org/10.57034/2618723x-2022-04-01","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124905256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2022-02-07
M. Makarova, A. Matichin, A.A. Maticina, V. Makarov
{"title":"Animal choice strategy for research. Report 1: animal choice based on phylogenic relationships","authors":"M. Makarova, A. Matichin, A.A. Maticina, V. Makarov","doi":"10.29296/2618723x-2022-02-07","DOIUrl":"https://doi.org/10.29296/2618723x-2022-02-07","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125233869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：