首页 > 最新文献

Hormone Molecular Biology and Clinical Investigation最新文献

英文 中文
Does dexamethasone inhibit glucose oxidase: an analysis in kinetics and molecular study. 地塞米松抑制葡萄糖氧化酶:动力学和分子研究的分析。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0030
Edris Majd, Dariush Minai-Tehrani, Hamidreza Mollasalehi

Objectives: Glucose oxidase is an enzyme that is widely used in biosensors, especially kits for measuring blood sugar. Many diabetics use this type of kit to determine their blood sugar level. Aspergillus niger is the most important source of glucose oxidase for use in biosensors. Diabetes causes secondary diseases in patients for which medications are prescribed to improve them. Dexamethasone, a corticosteroid, is one of the drugs prescribed to diabetics to cure some secondary diseases. In this study, the effect of this drug on glucose oxidase was investigated from a kinetic and molecular point of view.

Methods: In this study, the kinetics of drug binding to the enzyme was measured and the type of inhibition was determined by Lineweaver-Burk plot. The Ki value of the drug was determined by drawing the secondary curve. Using fluorescence spectrophotometry and molecular docking, the binding of the drug to the enzyme was confirmed.

Results: The results showed that the drug inhibits the enzyme non-competitively. Determining the kinetics parameters of the drug-enzyme interaction showed that the drug acts as a potent inhibitor. Study at the molecular level by fluorescence spectrophotometer showed that the drug attachment alters the enzyme conformation to more compaction. In silico results showed that the drug is placed between two helices that are outside the active site and binds to the enzyme by three hydrogen bonds.

Conclusions: The result of this study is useful because it suggests that in diabetic patients taking dexamethasone, the amount of glucose declared by the kit may not be real due to the inhibition of glucose oxidase.

目的:葡萄糖氧化酶是一种广泛应用于生物传感器的酶,特别是用于测量血糖的试剂盒。许多糖尿病患者使用这种试剂盒来测定他们的血糖水平。黑曲霉是生物传感器中葡萄糖氧化酶最重要的来源。糖尿病会导致患者继发性疾病,这些疾病需要药物来改善。地塞米松是一种皮质类固醇,是糖尿病患者治疗一些继发性疾病的药物之一。本研究从动力学和分子的角度研究了该药物对葡萄糖氧化酶的作用。方法:采用Lineweaver-Burk图测定药物与该酶的结合动力学,并测定其抑制类型。通过绘制二次曲线测定药物的Ki值。利用荧光分光光度法和分子对接法,证实了药物与酶的结合。结果:该药物对该酶具有非竞争性抑制作用。测定药物-酶相互作用的动力学参数表明,该药物是一种有效的抑制剂。荧光分光光度计在分子水平上的研究表明,药物的附着改变了酶的构象,使其更紧实。计算机结果表明,药物被置于活性位点外的两个螺旋之间,并通过三个氢键与酶结合。结论:本研究的结果是有用的,因为它表明,在糖尿病患者服用地塞米松,试剂盒所宣布的葡萄糖量可能不是真实的,由于葡萄糖氧化酶的抑制。
{"title":"Does dexamethasone inhibit glucose oxidase: an analysis in kinetics and molecular study.","authors":"Edris Majd,&nbsp;Dariush Minai-Tehrani,&nbsp;Hamidreza Mollasalehi","doi":"10.1515/hmbci-2022-0030","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0030","url":null,"abstract":"<p><strong>Objectives: </strong>Glucose oxidase is an enzyme that is widely used in biosensors, especially kits for measuring blood sugar. Many diabetics use this type of kit to determine their blood sugar level. Aspergillus niger is the most important source of glucose oxidase for use in biosensors. Diabetes causes secondary diseases in patients for which medications are prescribed to improve them. Dexamethasone, a corticosteroid, is one of the drugs prescribed to diabetics to cure some secondary diseases. In this study, the effect of this drug on glucose oxidase was investigated from a kinetic and molecular point of view.</p><p><strong>Methods: </strong>In this study, the kinetics of drug binding to the enzyme was measured and the type of inhibition was determined by Lineweaver-Burk plot. The Ki value of the drug was determined by drawing the secondary curve. Using fluorescence spectrophotometry and molecular docking, the binding of the drug to the enzyme was confirmed.</p><p><strong>Results: </strong>The results showed that the drug inhibits the enzyme non-competitively. Determining the kinetics parameters of the drug-enzyme interaction showed that the drug acts as a potent inhibitor. Study at the molecular level by fluorescence spectrophotometer showed that the drug attachment alters the enzyme conformation to more compaction. In silico results showed that the drug is placed between two helices that are outside the active site and binds to the enzyme by three hydrogen bonds.</p><p><strong>Conclusions: </strong>The result of this study is useful because it suggests that in diabetic patients taking dexamethasone, the amount of glucose declared by the kit may not be real due to the inhibition of glucose oxidase.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"5-10"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9208060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of the ratio of serum progesterone level to oocyte count on the day of IVF-ICSI injection on pregnancy outcomes in HCG cycles. 体外受精- icsi注射当日血清孕酮/卵母细胞数比值对HCG周期妊娠结局的影响。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0049
Azita Khahani Namin, Farnaz Mohammadian, Lida Garrosi, Shabnam Tofighi

Background: There has been several discussion and debates regarding the possible setremental influence of elevated serum progesterone (SP) on the day of human chorionic gonadotropin (hCG) administration. Our study aims to assess progestron to oocyte rates for assessing CPR and live birth rate (LBR) in IVF cycles and review previous articles.

Methods: In this prospective cohort study, women under ovulation induction through IVF-ICSI using the GnRH-antagonist protocol were studied. Five specific indicators were considered to assess pregnancy outcome. The statistical analysis was done using SPSS software.

Results: In the present research, 78 patients underwent IVF. The cut-off points for each of the three parameters were 1.2 (with a sensitivity of 65.4% and a specificity of 54%), 6.5 (with a sensitivity of 73.1% and a specificity of 56%), and 0.16 (with a sensitivity of 65.4% and a specificity of 60%, respectively). Only the number of oocytes (area below the curve of 0.64) was able to predict clinical pregnancy. The cut-off point for this parameter was 6.5 (with a sensitivity of 74.1% and a specificity of 66%). On the other hand, none of the parameters were able to predict live birth.

Conclusions: The findings of this study should assist in the clinical management of patients with high SP on the day of HCG administration. We recommend, that the ratio of SP to oocyte is a useful parameter for refining the criteria of patients who have had embryo freezing of all embryos (by selective freezing) and subsequent transfer of frozen embryos.

背景:关于血清黄体酮(SP)升高对人绒毛膜促性腺激素(hCG)给药当日可能产生的影响,已有一些讨论和争论。我们的研究旨在评估孕激素对卵母细胞的比率,以评估体外受精周期中CPR和活产率(LBR),并回顾以往的文章。方法:在这项前瞻性队列研究中,研究了使用gnrh拮抗剂方案通过IVF-ICSI诱导排卵的妇女。考虑了五个具体指标来评估妊娠结局。采用SPSS软件进行统计分析。结果:本组78例患者行体外受精。三个参数的分界点分别为1.2(敏感性为65.4%,特异性为54%)、6.5(敏感性为73.1%,特异性为56%)和0.16(敏感性为65.4%,特异性为60%)。只有卵母细胞数(曲线下方面积为0.64)能够预测临床妊娠。该参数的分界点为6.5(敏感性为74.1%,特异性为66%)。另一方面,没有一个参数能够预测活产。结论:本研究结果有助于HCG给药当日高SP患者的临床管理。我们建议,SP与卵母细胞的比例是一个有用的参数,可用于完善所有胚胎冷冻(通过选择性冷冻)和随后移植冷冻胚胎的患者的标准。
{"title":"The effect of the ratio of serum progesterone level to oocyte count on the day of IVF-ICSI injection on pregnancy outcomes in HCG cycles.","authors":"Azita Khahani Namin,&nbsp;Farnaz Mohammadian,&nbsp;Lida Garrosi,&nbsp;Shabnam Tofighi","doi":"10.1515/hmbci-2022-0049","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0049","url":null,"abstract":"<p><strong>Background: </strong>There has been several discussion and debates regarding the possible setremental influence of elevated serum progesterone (SP) on the day of human chorionic gonadotropin (hCG) administration. Our study aims to assess progestron to oocyte rates for assessing CPR and live birth rate (LBR) in IVF cycles and review previous articles.</p><p><strong>Methods: </strong>In this prospective cohort study, women under ovulation induction through IVF-ICSI using the GnRH-antagonist protocol were studied. Five specific indicators were considered to assess pregnancy outcome. The statistical analysis was done using SPSS software.</p><p><strong>Results: </strong>In the present research, 78 patients underwent IVF. The cut-off points for each of the three parameters were 1.2 (with a sensitivity of 65.4% and a specificity of 54%), 6.5 (with a sensitivity of 73.1% and a specificity of 56%), and 0.16 (with a sensitivity of 65.4% and a specificity of 60%, respectively). Only the number of oocytes (area below the curve of 0.64) was able to predict clinical pregnancy. The cut-off point for this parameter was 6.5 (with a sensitivity of 74.1% and a specificity of 66%). On the other hand, none of the parameters were able to predict live birth.</p><p><strong>Conclusions: </strong>The findings of this study should assist in the clinical management of patients with high SP on the day of HCG administration. We recommend, that the ratio of SP to oocyte is a useful parameter for refining the criteria of patients who have had embryo freezing of all embryos (by selective freezing) and subsequent transfer of frozen embryos.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"53-60"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9262829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Level of non-conventional lipid parameters and its comparative analysis with TSH in subclinical hypothyroidism. 亚临床甲状腺功能减退患者非常规脂质参数水平及其与TSH的比较分析。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0019
Sanjiv Kumar Bansal, Arpita Suri, Varsha Suryan, Naveen Kumar Singh, Smita Barman

Objectives: The objective of this study is to estimate lipid parameters in subclinical hypothyroidism and correlate it with TSH.

Methods: Forty newly diagnosed cases of subclinical hypothyroidism and Forty age and gender-matched healthy controls were recruited for the study. Blood samples were collected from them and serum lipid profile (i.e. HDL, LDL, TG, serum total cholesterol) of the subjects was estimated by standard photometric methods in a fully auto-analyzer (MINDRAY BS-300) using commercially available kits and VLDL cholesterol was calculated using the Friedewald's formula. While serum Ox-LDL, Lipoprotein A, Apolipoprotein A1 and Apo B were estimated by using commercial kit based on enzyme-linked immmunosorbent assay.

Results: The parameters such as Oxidized low-density lipoprotein (Ox-LDL), lipoprotein (a), apolipoprotein A1, apolipoprotein B and small dense lipoprotein (sd LDL) were significantly increased in subclinical hypothyroid cases when compared with the control subjects (p<0.0001). In present study results showed significant positive correlations of serum thyroid stimulating hormone (TSH) with Ox-LDL (r=0.85, p<0.01), sd LDL (r=0.71, p<0.01).

Conclusions: The present study focuses on the role of Ox-LDL, sd-LDL Lipoprotein A, Apolipoprotein A1 and Apo B that are sensitive indicators of atherogenic dyslipidemia in subclinical hypothyroidism and can serve as a better & novel risk factor for CAD.

目的:本研究的目的是估计亚临床甲状腺功能减退症的脂质参数及其与TSH的相关性。方法:招募40例新诊断的亚临床甲状腺功能减退患者和40例年龄、性别匹配的健康对照者。采集血样,在全自动分析仪(MINDRAY BS-300)中使用市售试剂盒,用标准光度法估计受试者的血脂(即HDL、LDL、TG、血清总胆固醇),用弗里德瓦尔德公式计算VLDL胆固醇。血清Ox-LDL、脂蛋白A、载脂蛋白A1和载脂蛋白B采用商用试剂盒酶联免疫吸附法测定。结果:亚临床甲状腺功能减退患者的氧化低密度脂蛋白(Ox-LDL)、脂蛋白(a)、载脂蛋白A1、载脂蛋白B、小密度脂蛋白(sd LDL)等指标均较对照组显著升高(p)。Ox-LDL、sd-LDL脂蛋白A、载脂蛋白A1和载脂蛋白B作为动脉粥样硬化性血脂异常的敏感指标在亚临床甲状腺功能减退中的作用,可作为冠心病更好的新危险因素。
{"title":"Level of non-conventional lipid parameters and its comparative analysis with TSH in subclinical hypothyroidism.","authors":"Sanjiv Kumar Bansal,&nbsp;Arpita Suri,&nbsp;Varsha Suryan,&nbsp;Naveen Kumar Singh,&nbsp;Smita Barman","doi":"10.1515/hmbci-2022-0019","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0019","url":null,"abstract":"<p><strong>Objectives: </strong>The objective of this study is to estimate lipid parameters in subclinical hypothyroidism and correlate it with TSH.</p><p><strong>Methods: </strong>Forty newly diagnosed cases of subclinical hypothyroidism and Forty age and gender-matched healthy controls were recruited for the study. Blood samples were collected from them and serum lipid profile (i.e. HDL, LDL, TG, serum total cholesterol) of the subjects was estimated by standard photometric methods in a fully auto-analyzer (MINDRAY BS-300) using commercially available kits and VLDL cholesterol was calculated using the Friedewald's formula. While serum Ox-LDL, Lipoprotein A, Apolipoprotein A1 and Apo B were estimated by using commercial kit based on enzyme-linked immmunosorbent assay.</p><p><strong>Results: </strong>The parameters such as Oxidized low-density lipoprotein (Ox-LDL), lipoprotein (a), apolipoprotein A1, apolipoprotein B and small dense lipoprotein (sd LDL) were significantly increased in subclinical hypothyroid cases when compared with the control subjects (p<0.0001). In present study results showed significant positive correlations of serum thyroid stimulating hormone (TSH) with Ox-LDL (r=0.85, p<0.01), sd LDL (r=0.71, p<0.01).</p><p><strong>Conclusions: </strong>The present study focuses on the role of Ox-LDL, sd-LDL Lipoprotein A, Apolipoprotein A1 and Apo B that are sensitive indicators of atherogenic dyslipidemia in subclinical hypothyroidism and can serve as a better & novel risk factor for CAD.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"61-65"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
COVID-19: a viewpoint from hepatic perspective. 从肝脏角度看COVID-19。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0026
Abhijeet Brizawasi, Ashok Kumar Ahirwar, Prabhat, Kirti Kaim, Pradeep Ahirwar, Rajani Kumawat, Jitender Prasad

Coronaviruses as such are known since last century. The name is derived from their shape which has crown (corona) like radiating spikes. The recent one however is a different one from the Coronavirus involved in SARS (2002-2004) and MERS (2012) in being highly infectious. Initially COVID 19 had a high case fatality rate which has now decreased to a significant extent. Many cases of COVID 19 are asymptomatic with a significant number of positive cases developing a triad of fever, breathlessness and GI symptoms. Recent travel increases the probability of infection. The pathogenesis involves ACE 2 receptors. So, it has been found that there are more cases and mortality among hypertensive individuals. Even higher among the people who use ACE inhibitor in comparison to those who use other anti-hypertensive drugs. Treatment is usually symptomatic. Antiviral drugs and vaccines against COVID-19 are being used. Deranged liver enzymes are common in COVID-19, however, serious liver injury is not much documented. Liver injury is either due to disease itself or due to antiviral drugs. Extra care like strict social distancing, avoiding unnecessary contact is needed for those with autoimmune hepatitis, liver cancer and those who are in immunosuppression because of a scheduled or already liver transplant. Further research is definitely needed in this field. The upcoming researches should also focus on liver injuries associated with disease course and derangements arising as side effects of treatment of COVID-19.

冠状病毒自上个世纪以来就为人所知。这个名字来源于它们的形状,有冠(日冕)像辐射的尖刺。然而,最近的冠状病毒与SARS(2002-2004年)和MERS(2012年)中的冠状病毒不同,传染性很强。最初,COVID - 19的病死率很高,现在已经大幅下降。许多COVID - 19病例无症状,大量阳性病例出现发烧、呼吸困难和胃肠道症状。最近的旅行增加了感染的可能性。其发病机制与ACE 2受体有关。因此,高血压患者的发病率和死亡率更高。与使用其他抗高血压药物的人相比,使用ACE抑制剂的人甚至更高。治疗通常是有症状的。目前正在使用针对COVID-19的抗病毒药物和疫苗。肝酶紊乱在COVID-19中很常见,但严重的肝损伤并没有太多记录。肝损伤要么是由于疾病本身,要么是由于抗病毒药物。对于那些患有自身免疫性肝炎、肝癌以及因计划或已经进行肝移植而处于免疫抑制状态的人来说,需要额外的照顾,比如严格的社交距离,避免不必要的接触。这一领域还需要进一步的研究。今后的研究也应该集中在与病程相关的肝损伤和治疗副作用引起的紊乱上。
{"title":"COVID-19: a viewpoint from hepatic perspective.","authors":"Abhijeet Brizawasi,&nbsp;Ashok Kumar Ahirwar,&nbsp;Prabhat,&nbsp;Kirti Kaim,&nbsp;Pradeep Ahirwar,&nbsp;Rajani Kumawat,&nbsp;Jitender Prasad","doi":"10.1515/hmbci-2022-0026","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0026","url":null,"abstract":"<p><p>Coronaviruses as such are known since last century. The name is derived from their shape which has crown (corona) like radiating spikes. The recent one however is a different one from the Coronavirus involved in SARS (2002-2004) and MERS (2012) in being highly infectious. Initially COVID 19 had a high case fatality rate which has now decreased to a significant extent. Many cases of COVID 19 are asymptomatic with a significant number of positive cases developing a triad of fever, breathlessness and GI symptoms. Recent travel increases the probability of infection. The pathogenesis involves ACE 2 receptors. So, it has been found that there are more cases and mortality among hypertensive individuals. Even higher among the people who use ACE inhibitor in comparison to those who use other anti-hypertensive drugs. Treatment is usually symptomatic. Antiviral drugs and vaccines against COVID-19 are being used. Deranged liver enzymes are common in COVID-19, however, serious liver injury is not much documented. Liver injury is either due to disease itself or due to antiviral drugs. Extra care like strict social distancing, avoiding unnecessary contact is needed for those with autoimmune hepatitis, liver cancer and those who are in immunosuppression because of a scheduled or already liver transplant. Further research is definitely needed in this field. The upcoming researches should also focus on liver injuries associated with disease course and derangements arising as side effects of treatment of COVID-19.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"97-103"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9208539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Comparative evaluation of INF-γ as an immunological healing marker based on anti-tubercular treatment among diabetic and non-diabetic pulmonary tuberculosis patients. nf -γ作为免疫愈合标志物在糖尿病和非糖尿病肺结核患者抗结核治疗中的比较评价。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0031
Birendra Kumar Yadav, Ashok Kumar Shah, Busi Karunanand, Dharampal Singh Sudan, Monika Sharma

Objectives: Tuberculosis is an infectious airborne disease caused by Mycobacterium tuberculosis. Pulmonary tuberculosis is the ninth most frequent complication of diabetes mellitus. The co-existence of TB and DM in patient causes severe TB symptoms, modify radiological findings, slower response to treatment outcomes and prognosis. IFN-γ is the key cytokine which play role in the protective immune response against mycobacterium infection. The main function of IFN-γ is macrophage activation which is able to exert its microbicidal functions. Estimation and comparison of pre and post treatment serum IFN-γ among pulmonary tuberculosis among diabetic and non-diabetic patients.

Methods: The study was conducted in the Departments of Biochemistry and Pulmonary Medicine, FMHS, SGT University, Budhera, Gurugram and District TB Centre, Gurugram, Haryana, India. In this study, 100 newly diagnosed PTB patients without diabetes mellitus and 100 newly diagnosed PTB patients with diabetes mellitus (PTB-DM) above 15 years of age were included after obtaining written consent. 5 mL venous blood was collected from patients of pre and post anti-tubercular treatment. The level of IFN-γ was measured by ELISA method.

Results: The circulating level of IFN-γ in PTB patients was significantly decreased in post-treatment (25.53 ± 6.12 pg/mL) compared to pre-treatment (58.76 ± 16.02 pg/mL) with t-value 32.03 and p-value <0.001. The circulating level of IFN-γ in PTB-DM patients was significantly decreased in post treatment (29.11 ± 7.41 pg/mL) compared to pre-treatment (44.14 ± 10.85 pg/mL) with t-value 31.35 and p-value <0.001. In the present study, level of IFN-γ in pre-treatment PTB patients (58.76 ± 16.02 pg/mL) was significantly raised compared to PTB-DM patients (44.14 ± 10.85 pg/mL) with t-value 7.55 and p-value <0.001. However, level of IFN-γ in post-treatment PTB patients (25.53 ± 6.12 pg/mL) was significantly low compared to PTB-DM patients (29.11 ± 7.41 pg/mL) with t-value 3.71 and p-value <0.001.

Conclusions: The decreased level of IFN-γ in post-treatment compared to pre-treatment in both PTB and PTB-DM patients had shown efficacy of anti-tubercular treatment. However, the post treatment level of IFN-γ was high in PTB-DM patients compared to PTB patients which verified that effect of ATT was low in PTB-DM.

目的:结核病是由结核分枝杆菌引起的一种空气传播传染病。肺结核是糖尿病的第九大常见并发症。患者结核和糖尿病的共存导致严重的结核症状,改变放射学表现,对治疗结果和预后的反应较慢。IFN-γ是在抗分枝杆菌感染的保护性免疫应答中起关键作用的细胞因子。IFN-γ的主要功能是激活巨噬细胞,从而发挥其杀灭微生物的功能。糖尿病与非糖尿病肺结核患者治疗前后血清IFN-γ的测定与比较方法:研究在印度哈里亚纳邦古尔格拉姆省布德拉市SGT大学FMHS生物化学系和肺医学系以及古尔格拉姆地区结核病中心进行。本研究纳入100例新诊断的无糖尿病PTB患者和100例15岁以上新诊断的PTB合并糖尿病(PTB- dm)患者,经书面同意。采集抗结核治疗前后患者静脉血5 mL。ELISA法检测IFN-γ水平。结果:PTB患者治疗后血清IFN-γ水平较治疗前(58.76±16.02 pg/mL)显著降低(25.53±6.12 pg/mL), t值为32.03,p值为p值。结论:PTB和PTB- dm患者治疗后血清IFN-γ水平较治疗前明显降低,显示出抗结核治疗的效果。然而,与PTB患者相比,PTB- dm患者治疗后的IFN-γ水平较高,这证实了ATT对PTB- dm的影响较低。
{"title":"Comparative evaluation of INF-γ as an immunological healing marker based on anti-tubercular treatment among diabetic and non-diabetic pulmonary tuberculosis patients.","authors":"Birendra Kumar Yadav,&nbsp;Ashok Kumar Shah,&nbsp;Busi Karunanand,&nbsp;Dharampal Singh Sudan,&nbsp;Monika Sharma","doi":"10.1515/hmbci-2022-0031","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0031","url":null,"abstract":"<p><strong>Objectives: </strong>Tuberculosis is an infectious airborne disease caused by <i>Mycobacterium tuberculosis</i>. Pulmonary tuberculosis is the ninth most frequent complication of diabetes mellitus. The co-existence of TB and DM in patient causes severe TB symptoms, modify radiological findings, slower response to treatment outcomes and prognosis. IFN-γ is the key cytokine which play role in the protective immune response against mycobacterium infection. The main function of IFN-γ is macrophage activation which is able to exert its microbicidal functions. Estimation and comparison of pre and post treatment serum IFN-γ among pulmonary tuberculosis among diabetic and non-diabetic patients.</p><p><strong>Methods: </strong>The study was conducted in the Departments of Biochemistry and Pulmonary Medicine, FMHS, SGT University, Budhera, Gurugram and District TB Centre, Gurugram, Haryana, India. In this study, 100 newly diagnosed PTB patients without diabetes mellitus and 100 newly diagnosed PTB patients with diabetes mellitus (PTB-DM) above 15 years of age were included after obtaining written consent. 5 mL venous blood was collected from patients of pre and post anti-tubercular treatment. The level of IFN-γ was measured by ELISA method.</p><p><strong>Results: </strong>The circulating level of IFN-γ in PTB patients was significantly decreased in post-treatment (25.53 ± 6.12 pg/mL) compared to pre-treatment (58.76 ± 16.02 pg/mL) with t-value 32.03 and p-value <0.001. The circulating level of IFN-γ in PTB-DM patients was significantly decreased in post treatment (29.11 ± 7.41 pg/mL) compared to pre-treatment (44.14 ± 10.85 pg/mL) with t-value 31.35 and p-value <0.001. In the present study, level of IFN-γ in pre-treatment PTB patients (58.76 ± 16.02 pg/mL) was significantly raised compared to PTB-DM patients (44.14 ± 10.85 pg/mL) with t-value 7.55 and p-value <0.001. However, level of IFN-γ in post-treatment PTB patients (25.53 ± 6.12 pg/mL) was significantly low compared to PTB-DM patients (29.11 ± 7.41 pg/mL) with t-value 3.71 and p-value <0.001.</p><p><strong>Conclusions: </strong>The decreased level of IFN-γ in post-treatment compared to pre-treatment in both PTB and PTB-DM patients had shown efficacy of anti-tubercular treatment. However, the post treatment level of IFN-γ was high in PTB-DM patients compared to PTB patients which verified that effect of ATT was low in PTB-DM.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"33-37"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9208532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Potential role of endoplasmic reticulum stress in the pathophysiology of polycystic ovary syndrome. 内质网应激在多囊卵巢综合征病理生理中的潜在作用。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0051
Beatriz Alexandre-Santos, Felipe Figuerôa Tassinari Rocha Martins, Larissa da Silva Gonçalves, Clara de Oliveira Guimarães, Fernanda Carla-Ruggiero, D'Angelo Carlo Magliano

Polycystic ovary syndrome (PCOS) is an endocrine disorder that affects million women worldwide, presenting a complex pathophysiology that has not been fully elucidated yet. Recently, it has been suggested that PCOS triggers the endoplasmic reticulum (ER) stress, thus being associated with unfolded protein response (UPR) activation. Indeed, the UPR response has been associated with several pathological conditions, including in the reproductive system. Several studies demonstrated that ovarian UPR markers are upregulated in PCOS, being associated with worst ovarian outcomes, and this was ameliorated by ER stress inhibition. In this review, we aim to summarize the main findings from previous studies covering this topic, in an attempt to clarify the potential role of ER stress and the UPR response in the pathophysiology of PCOS.

多囊卵巢综合征(PCOS)是一种影响全球数百万女性的内分泌疾病,其复杂的病理生理机制尚未完全阐明。近年来,有研究认为多囊卵巢综合征触发内质网应激,从而与未折叠蛋白反应(UPR)激活有关。事实上,普遍定期审议反应与包括生殖系统在内的几种病理状况有关。一些研究表明,卵巢UPR标志物在PCOS中上调,与卵巢预后最差有关,内质网应激抑制可改善这种情况。在本文中,我们旨在总结这一主题的主要研究成果,试图阐明内质网应激和UPR反应在多囊卵巢综合征病理生理中的潜在作用。
{"title":"Potential role of endoplasmic reticulum stress in the pathophysiology of polycystic ovary syndrome.","authors":"Beatriz Alexandre-Santos,&nbsp;Felipe Figuerôa Tassinari Rocha Martins,&nbsp;Larissa da Silva Gonçalves,&nbsp;Clara de Oliveira Guimarães,&nbsp;Fernanda Carla-Ruggiero,&nbsp;D'Angelo Carlo Magliano","doi":"10.1515/hmbci-2022-0051","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0051","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) is an endocrine disorder that affects million women worldwide, presenting a complex pathophysiology that has not been fully elucidated yet. Recently, it has been suggested that PCOS triggers the endoplasmic reticulum (ER) stress, thus being associated with unfolded protein response (UPR) activation. Indeed, the UPR response has been associated with several pathological conditions, including in the reproductive system. Several studies demonstrated that ovarian UPR markers are upregulated in PCOS, being associated with worst ovarian outcomes, and this was ameliorated by ER stress inhibition. In this review, we aim to summarize the main findings from previous studies covering this topic, in an attempt to clarify the potential role of ER stress and the UPR response in the pathophysiology of PCOS.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"105-112"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9209906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-proliferation effects of Apatinib in combination with Curcumin in breast cancer cells. 阿帕替尼联合姜黄素对乳腺癌细胞的抗增殖作用。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0036
Mahdi Farhoudi Sefidan Jadid, Gholamreza Jahangirzadehd, Javad Behroozi

Objectives: Despite remarkable development of new therapeutic strategies to improve survival rates and treatment of patients with cancer, there are still many limitations in management of patients with distant metastasis breast cancer. Therefore, the aim of this study was to investigate a novel method to enhance therapeutic efficacy of Apatinib (as a chemotherapeutic agent) by co-administration of Curcumin (as a bioactive herbal compound) in breast cancer treatment.

Methods: Effects of Apatinib, Curcumin, and their combinations (Apa-Cur) was evaluated on viability and proliferation of breast cell line (MCF7) by MTT assay. Moreover, effects of Apatinib, Curcumin, and Apa-Cur was investigated on apoptosis rate in the cancer cells. Expression levels of apoptosis-related genes (BAX, SMAC, BCL2, and SURVIVIN) in treated cancer cells and untreated controls were evaluated using the Real-Time PCR method.

Results: The obtained results showed that all treatments of Apatinib, Curcumin, and Apa-Cur significantly decreased viability and proliferation of the breast cancer cells in a concentration- and time-dependent manner. However, anti-proliferation activity of Apa-Cur combination was significantly higher than Apatinib and Curcumin treatment alone. In addition, Apatinib, Curcumin, and Apa-Cur increased apoptosis percentage in the treated cancer cells through regulation of apoptosis-related genes expression.

Conclusions: In general, Apa-Cur combination therapy exerts more profound anti-proliferation effects on breast cancer cell than Apatinib or Curcumin monotherapy. However, further studies are required to identify other possible signaling pathways and mechanisms involved in the anticancer effects of Apatinib, Curcumin, and Apa-Cur.

目的:尽管新的治疗策略在提高癌症患者的生存率和治疗方面取得了显著进展,但在远处转移性乳腺癌患者的治疗方面仍存在许多局限性。因此,本研究的目的是探索一种新的方法,通过与姜黄素(一种生物活性草药化合物)共同给药来提高阿帕替尼(作为化疗药物)在乳腺癌治疗中的疗效。方法:采用MTT法观察阿帕替尼、姜黄素及其联合用药(Apa-Cur)对乳腺癌MCF7细胞活力和增殖的影响。此外,我们还研究了阿帕替尼、姜黄素和Apa-Cur对肿瘤细胞凋亡率的影响。使用Real-Time PCR方法评估处理过的癌细胞和未处理的对照组中凋亡相关基因(BAX、SMAC、BCL2和SURVIVIN)的表达水平。结果:得到的结果显示,阿帕替尼、姜黄素和Apa-Cur处理均能显著降低乳腺癌细胞的活力和增殖,且呈浓度依赖性和时间依赖性。然而,Apa-Cur联合治疗的抗增殖活性明显高于阿帕替尼和姜黄素单独治疗。此外,阿帕替尼、姜黄素和Apa-Cur通过调节凋亡相关基因的表达,增加了治疗后癌细胞的凋亡百分比。结论:总的来说,阿帕替尼和姜黄素联合治疗对乳腺癌细胞的抗增殖作用比阿帕替尼或姜黄素单药更深刻。然而,需要进一步的研究来确定阿帕替尼、姜黄素和Apa-Cur抗癌作用中涉及的其他可能的信号通路和机制。
{"title":"Anti-proliferation effects of Apatinib in combination with Curcumin in breast cancer cells.","authors":"Mahdi Farhoudi Sefidan Jadid,&nbsp;Gholamreza Jahangirzadehd,&nbsp;Javad Behroozi","doi":"10.1515/hmbci-2022-0036","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0036","url":null,"abstract":"<p><strong>Objectives: </strong>Despite remarkable development of new therapeutic strategies to improve survival rates and treatment of patients with cancer, there are still many limitations in management of patients with distant metastasis breast cancer. Therefore, the aim of this study was to investigate a novel method to enhance therapeutic efficacy of Apatinib (as a chemotherapeutic agent) by co-administration of Curcumin (as a bioactive herbal compound) in breast cancer treatment.</p><p><strong>Methods: </strong>Effects of Apatinib, Curcumin, and their combinations (Apa-Cur) was evaluated on viability and proliferation of breast cell line (MCF7) by MTT assay. Moreover, effects of Apatinib, Curcumin, and Apa-Cur was investigated on apoptosis rate in the cancer cells. Expression levels of apoptosis-related genes (<i>BAX</i>, <i>SMAC</i>, <i>BCL2</i>, and <i>SURVIVIN</i>) in treated cancer cells and untreated controls were evaluated using the Real-Time PCR method.</p><p><strong>Results: </strong>The obtained results showed that all treatments of Apatinib, Curcumin, and Apa-Cur significantly decreased viability and proliferation of the breast cancer cells in a concentration- and time-dependent manner. However, anti-proliferation activity of Apa-Cur combination was significantly higher than Apatinib and Curcumin treatment alone. In addition, Apatinib, Curcumin, and Apa-Cur increased apoptosis percentage in the treated cancer cells through regulation of apoptosis-related genes expression.</p><p><strong>Conclusions: </strong>In general, Apa-Cur combination therapy exerts more profound anti-proliferation effects on breast cancer cell than Apatinib or Curcumin monotherapy. However, further studies are required to identify other possible signaling pathways and mechanisms involved in the anticancer effects of Apatinib, Curcumin, and Apa-Cur.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"27-32"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9562087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Thyrotropin and body mass index, are they related? 促甲状腺激素和体重指数有关系吗?
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0002
Catarina R Ivo, Vitória Duarte, David Veríssimo, João Silva, Dolores Passos, Luís Lopes, João Jácome de Castro, Mafalda Marcelino

Objectives: It is well recognized that overt thyroid dysfunction is associated with changes in body mass index (BMI). However, there is ongoing debate regarding the influence of thyroid stimulating hormone (TSH) on BMI, in euthyroid subjects. The aim of this study is to examine the association of TSH with BMI in an outpatient population without evidence of thyroid disease.

Methods: Cross-sectional study conducted in an Endocrinology Department. We identified the latest TSH and BMI measurements in 923 patients from the reference euthyroid population. All patients with positive thyroid autoimmunity and nodules were excluded. We performed a linear regression analysis using SPSSv.025.

Results: 923 adult patients were evaluated. 79.4% were males, with a mean age of 67.6 years old. Mean TSH level was 1.78 mIU/L and mean BMI was 29.2 kg/m2. A significant negative correlation between serum TSH concentration and BMI was evident (p=0.04; r=-0.067). Statistical significance was lost when performing subgroup analysis, for males and females (p=0.19 and p=0.075), elderly (≥65 years) and non-elderly (p=0.55 and p=0.32) and also obese (BMI ≥30 kg/m2) and non-obese (p=0.39 and p=0.13).

Conclusions: The relationship between BMI and TSH is not consensual in the literature. This study included a large cohort sample of euthyroid patients, majority men and with negative autoimmunity. Our results support the hypothesis that variation in thyroid status within the normal range, could have a negative effect on BMI, contrary to most published studies.

目的:众所周知,明显的甲状腺功能障碍与身体质量指数(BMI)的变化有关。然而,在甲状腺功能正常的受试者中,关于促甲状腺激素(TSH)对BMI的影响一直存在争议。本研究的目的是在没有甲状腺疾病证据的门诊人群中检查TSH与BMI的关系。方法:横断面研究在内分泌科进行。我们确定了923例参考甲状腺功能正常人群的最新TSH和BMI测量值。排除所有甲状腺自身免疫阳性和结节的患者。我们使用SPSSv.025进行线性回归分析。结果:对923例成人患者进行了评价。79.4%为男性,平均年龄67.6岁。平均TSH水平为1.78 mIU/L,平均BMI为29.2 kg/m2。血清TSH浓度与BMI呈显著负相关(p=0.04;r = -0.067)。在进行亚组分析时,男性和女性(p=0.19和p=0.075)、老年人(≥65岁)和非老年人(p=0.55和p=0.32)、肥胖(BMI≥30 kg/m2)和非肥胖(p=0.39和p=0.13)均无统计学意义。结论:BMI和TSH之间的关系在文献中并不是双方同意的。本研究纳入了大量甲状腺功能正常患者的队列样本,大多数为男性,自身免疫阴性。我们的研究结果支持了一个假设,即甲状腺状态在正常范围内的变化可能会对BMI产生负面影响,这与大多数已发表的研究相反。
{"title":"Thyrotropin and body mass index, are they related?","authors":"Catarina R Ivo,&nbsp;Vitória Duarte,&nbsp;David Veríssimo,&nbsp;João Silva,&nbsp;Dolores Passos,&nbsp;Luís Lopes,&nbsp;João Jácome de Castro,&nbsp;Mafalda Marcelino","doi":"10.1515/hmbci-2022-0002","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0002","url":null,"abstract":"<p><strong>Objectives: </strong>It is well recognized that overt thyroid dysfunction is associated with changes in body mass index (BMI). However, there is ongoing debate regarding the influence of thyroid stimulating hormone (TSH) on BMI, in euthyroid subjects. The aim of this study is to examine the association of TSH with BMI in an outpatient population without evidence of thyroid disease.</p><p><strong>Methods: </strong>Cross-sectional study conducted in an Endocrinology Department. We identified the latest TSH and BMI measurements in 923 patients from the reference euthyroid population. All patients with positive thyroid autoimmunity and nodules were excluded. We performed a linear regression analysis using SPSSv.025.</p><p><strong>Results: </strong>923 adult patients were evaluated. 79.4% were males, with a mean age of 67.6 years old. Mean TSH level was 1.78 mIU/L and mean BMI was 29.2 kg/m<sup>2</sup>. A significant negative correlation between serum TSH concentration and BMI was evident (p=0.04; r=-0.067). Statistical significance was lost when performing subgroup analysis, for males and females (p=0.19 and p=0.075), elderly (≥65 years) and non-elderly (p<i>=</i>0.55 and p<i>=</i>0.32) and also obese (BMI ≥30 kg/m<sup>2</sup>) and non-obese (p=0.39 and p=0.13)<i>.</i></p><p><strong>Conclusions: </strong>The relationship between BMI and TSH is not consensual in the literature. This study included a large cohort sample of euthyroid patients, majority men and with negative autoimmunity. Our results support the hypothesis that variation in thyroid status within the normal range, could have a negative effect on BMI, contrary to most published studies.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"1-4"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9208067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Modulatory role of prolactin in type 1 diabetes. 催乳素在1型糖尿病中的调节作用。
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0008
Edgar Ramos-Martínez, Ivan Ramos-Martínez, Jorge Valencia, Juan Carlos Ramos-Martínez, Luis Hernández-Zimbrón, Anaiza Rico-Luna, Eduardo Pérez-Campos, Laura Pérez-Campos Mayoral, Marco Cerbón

Objectives: Patients with type 1 diabetes mellitus have been reported to have elevated prolactin levels and a possible relationship between prolactin levels and the development of the disease has been proposed. However, some studies show that prolactin mediates beneficial functions in beta cells. Therefore, we review information on the roles of prolactin in type 1 diabetes mellitus.

Content: Here we summarize the functions of prolactin in the immune system and in pancreatic beta cells, in addition, we describe studies related to PRL levels, its regulation and alterations of secretion in patients with type 1 diabetes mellitus.

Summary: Studies in murine models have shown that prolactin protects beta cells from apoptosis, stimulates their proliferation and promotes pancreatic islet revascularization. In addition, some studies in patients with type 1 diabetes mellitus have shown that elevated prolactin levels correlate with better disease control.

Outlook: Prolactin treatment appears to be a promising strategy to improve beta-cell vascularization and proliferation in transplantation and immunotherapies.

目的:已报道1型糖尿病患者催乳素水平升高,并提出催乳素水平与疾病发展之间可能存在的关系。然而,一些研究表明,催乳素介导β细胞的有益功能。因此,我们对催乳素在1型糖尿病中的作用进行综述。内容:本文综述了催乳素在免疫系统和胰腺β细胞中的功能,并对1型糖尿病患者催乳素水平、调节及分泌改变的相关研究进行了综述。摘要:小鼠模型研究表明,催乳素可保护β细胞免于凋亡,刺激其增殖并促进胰岛血运重建。此外,一些对1型糖尿病患者的研究表明,催乳素水平升高与更好的疾病控制有关。展望:在移植和免疫治疗中,催乳素治疗似乎是一种有希望改善β细胞血管化和增殖的策略。
{"title":"Modulatory role of prolactin in type 1 diabetes.","authors":"Edgar Ramos-Martínez,&nbsp;Ivan Ramos-Martínez,&nbsp;Jorge Valencia,&nbsp;Juan Carlos Ramos-Martínez,&nbsp;Luis Hernández-Zimbrón,&nbsp;Anaiza Rico-Luna,&nbsp;Eduardo Pérez-Campos,&nbsp;Laura Pérez-Campos Mayoral,&nbsp;Marco Cerbón","doi":"10.1515/hmbci-2022-0008","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0008","url":null,"abstract":"<p><strong>Objectives: </strong>Patients with type 1 diabetes mellitus have been reported to have elevated prolactin levels and a possible relationship between prolactin levels and the development of the disease has been proposed. However, some studies show that prolactin mediates beneficial functions in beta cells. Therefore, we review information on the roles of prolactin in type 1 diabetes mellitus.</p><p><strong>Content: </strong>Here we summarize the functions of prolactin in the immune system and in pancreatic beta cells, in addition, we describe studies related to PRL levels, its regulation and alterations of secretion in patients with type 1 diabetes mellitus.</p><p><strong>Summary: </strong>Studies in murine models have shown that prolactin protects beta cells from apoptosis, stimulates their proliferation and promotes pancreatic islet revascularization. In addition, some studies in patients with type 1 diabetes mellitus have shown that elevated prolactin levels correlate with better disease control.</p><p><strong>Outlook: </strong>Prolactin treatment appears to be a promising strategy to improve beta-cell vascularization and proliferation in transplantation and immunotherapies.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"79-88"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9214442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene polymorphism of leptin and risk for heart disease, obesity, and high BMI: a systematic review and pooled analysis in adult obese subjects. 瘦素基因多态性与心脏病、肥胖和高BMI风险:成人肥胖受试者的系统回顾和汇总分析
IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-03-01 DOI: 10.1515/hmbci-2022-0020
Fatemeh Khaki-Khatibi, Behrouz Shademan, Reza Gholikhani-Darbroud, Alireza Nourazarian, Saeed Radagdam, Maghsoud Porzour

Objectives: Leptin polymorphism (LEP) has been associated with coronary heart disease (CAD), obesity, and high body mass index (BMI). However, we performed a systematic review and meta-analysis to discover the association because previous studies reached different conclusions.

Methods: Review Manager, version 5.3.5, and Stata, version 15.0, were used for statistical analysis. We calculated the effect size of the studies using the OR with the corresponding 95% CI, and two-sided (bilateral) p-values of 0.05 were considered significant. To determine heterogeneity among the selected studies, the Q test and I2 statistics were used. Meta-regression was used to examine the disease (heart disease, obesity, and high BMI) and heterogeneity between these subgroups.

Results: Eleven studies with 18,984 subjects were included in this study. The G-2548A (rs12112075), rs7799039, and A19G (rs2167270) polymorphisms of the leptin gene (but not the Lys656Asn (rs1805094) polymorphism) are associated with an increased risk of cardiovascular disease. Our pooled analysis revealed an association between the G-2548A (rs12112075) polymorphism and heart disease, high BMI, and obesity. This indicates that individuals carrying the AA allele are at an increased risk for heart disease, high BMI, and obesity. People with heart failure and coronary artery disease did not have the rs7799039 polymorphism or its alleles linked to them.

Conclusions: Combined analysis of data from current and published research suggests that the leptin gene polymorphisms G-2548A (rs12112075), rs7799039, and A19G (rs2167270) (but not the Lys656Asn (rs1805094) polymorphism) are associated with an increased risk of cardiovascular disease. Further research is needed to understand this association.

目的:瘦素多态性(LEP)与冠心病(CAD)、肥胖和高体重指数(BMI)相关。然而,由于之前的研究得出了不同的结论,我们进行了系统回顾和荟萃分析来发现这种关联。方法:采用Review Manager软件5.3.5版本,Stata软件15.0版本进行统计分析。我们使用OR和相应的95% CI计算研究的效应大小,双侧(双侧)p值0.05被认为是显著的。为了确定所选研究之间的异质性,使用Q检验和I2统计。meta回归用于检查疾病(心脏病、肥胖和高BMI)和这些亚组之间的异质性。结果:本研究共纳入11项研究,共18,984名受试者。瘦素基因的G-2548A (rs12112075)、rs7799039和A19G (rs2167270)多态性(而不是Lys656Asn (rs1805094)多态性)与心血管疾病风险增加相关。我们的汇总分析显示G-2548A (rs12112075)多态性与心脏病、高BMI和肥胖之间存在关联。这表明携带AA等位基因的人患心脏病、高BMI和肥胖的风险更高。患有心力衰竭和冠状动脉疾病的人没有rs7799039多态性或与其相关的等位基因。结论:当前和已发表研究数据的综合分析表明,瘦素基因多态性G-2548A (rs12112075)、rs7799039和A19G (rs2167270)(而不是Lys656Asn (rs1805094)多态性)与心血管疾病风险增加相关。需要进一步的研究来理解这种联系。
{"title":"Gene polymorphism of leptin and risk for heart disease, obesity, and high BMI: a systematic review and pooled analysis in adult obese subjects.","authors":"Fatemeh Khaki-Khatibi,&nbsp;Behrouz Shademan,&nbsp;Reza Gholikhani-Darbroud,&nbsp;Alireza Nourazarian,&nbsp;Saeed Radagdam,&nbsp;Maghsoud Porzour","doi":"10.1515/hmbci-2022-0020","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0020","url":null,"abstract":"<p><strong>Objectives: </strong>Leptin polymorphism (LEP) has been associated with coronary heart disease (CAD), obesity, and high body mass index (BMI). However, we performed a systematic review and meta-analysis to discover the association because previous studies reached different conclusions.</p><p><strong>Methods: </strong>Review Manager, version 5.3.5, and Stata, version 15.0, were used for statistical analysis. We calculated the effect size of the studies using the OR with the corresponding 95% CI, and two-sided (bilateral) p-values of 0.05 were considered significant. To determine heterogeneity among the selected studies, the Q test and I2 statistics were used. Meta-regression was used to examine the disease (heart disease, obesity, and high BMI) and heterogeneity between these subgroups.</p><p><strong>Results: </strong>Eleven studies with 18,984 subjects were included in this study. The G-2548A (rs12112075), rs7799039, and A19G (rs2167270) polymorphisms of the leptin gene (but not the Lys656Asn (rs1805094) polymorphism) are associated with an increased risk of cardiovascular disease. Our pooled analysis revealed an association between the G-2548A (rs12112075) polymorphism and heart disease, high BMI, and obesity. This indicates that individuals carrying the AA allele are at an increased risk for heart disease, high BMI, and obesity. People with heart failure and coronary artery disease did not have the rs7799039 polymorphism or its alleles linked to them.</p><p><strong>Conclusions: </strong>Combined analysis of data from current and published research suggests that the leptin gene polymorphisms G-2548A (rs12112075), rs7799039, and A19G (rs2167270) (but not the Lys656Asn (rs1805094) polymorphism) are associated with an increased risk of cardiovascular disease. Further research is needed to understand this association.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"44 1","pages":"11-20"},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9623460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
Hormone Molecular Biology and Clinical Investigation
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1