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Review: Extensive Potentiality of Selected Tropical Fruits from Indonesia 综述:印尼精选热带水果的广泛潜力
Pub Date : 2017-11-30 DOI: 10.22146/IFNP.28427
A. Ningrum, M. Schreiner
Indonesia has several biodiversities including tropical fruits.   The consumption of tropical fruits is increasing as this source can be used as a healthy source of several bioactive compounds to prevent severalnon-communicable diseases.  Tropical fruits are commonly used for the production of several food products e.g. juice, jams, jellies, cookies and other products.  The utilization of by product of tropical fruits have not been developed and promoted.  Although, several bioactive compounds are still present in tropical fruits by product.  This concept called as avalorization of by product to increase the economical value of several by product.  The purpose of this review isto promote several selected Indonesian tropical fruit and also to promote the potency of a by-product of tropical fruits in valorization concept.
印度尼西亚有多种生物多样性,包括热带水果。热带水果的消费量正在增加,因为这一来源可作为几种生物活性化合物的健康来源,以预防几种非传染性疾病。热带水果通常用于生产几种食品,如果汁、果酱、果冻、饼干和其他产品。热带水果副产品的利用还没有得到开发和推广。尽管如此,一些生物活性化合物仍然存在于热带水果副产品中。这个概念被称为副产品的雪崩,以增加几种副产品的经济价值。本综述的目的是推广几种精选的印度尼西亚热带水果,并在增值概念中促进热带水果副产品的效力。
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引用次数: 5
Kinetic Degradation of Myristicin, Elemicin, and Safrole in Encapsulated Banda Nutmeg (Myristica fragrans Houtt) Oleoresin 肉豆蔻油树脂对肉豆蔻素、Elemicin和红花的动力学降解
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24277
D. Amrih, C. Hidayat, Pudji Hastuti
Spices, in the form of oleoresin, have advantages as a flavoring agent. Nutmeg oleoresin compounds that play an important role in the formation of nutmeg flavor are myristicin, elemicin, and safrole. These compounds are not stable, so that the quality of oleoresin decreased easily. An alternative to overcome this problem is the encapsulation of oleoresin using spray drying techniques. The objective of this research was to evaluate the physicochemical properties of oleoresin with respect to the degradation of myristicin, elemicin, and safrole of nutmeg oleoresin. Microencapsulated nutmeg oleoresin was prepared using 12% WPC and 88% maltodextrin as encapsulate materials. It was stored in dark glass bottles at a various temperature, namely 30°C, 40°C, 50°C, 60°C, and 70°C, for 0, 4, 7, 10, 14, 21, 28, 35, 42, 49, 56 and 63 days. The content of myristicin, elemicin, and safrole were analysed. The results showed that myristicin, elemicin, and safrole in the encapsulated nutmeg oleoresin decreased during the storage until 28 days of storage and then they were relatively stable until 63 days. The activation energy of myristicin, elemicin, and safrole were 2.21 kJ/mol.K, 2.71 kJ/mol.K and 3.22 kJ/mol.K, respectively.
以油树脂形式存在的香料作为调味剂有其优点。肉豆蔻油树脂化合物在肉豆蔻风味的形成中起重要作用的是肉豆蔻素、榄香素和黄樟素。这些化合物不稳定,容易使油树脂的质量下降。克服这一问题的另一种方法是使用喷雾干燥技术对油树脂进行封装。本研究的目的是评价肉豆蔻油树脂在肉豆蔻油树脂中肉豆蔻素、榄香素和黄樟酚降解方面的理化性质。以12%木聚糖和88%麦芽糖糊精为包封材料,制备了肉豆蔻油树脂微胶囊。在30°C、40°C、50°C、60°C、70°C的不同温度下,在深色玻璃瓶中保存0、4、7、10、14、21、28、35、42、49、56、63天。分析了肉豆蔻素、榄香素、黄樟素的含量。结果表明:肉豆蔻油树脂中肉豆蔻素、榄香素和黄樟素含量在贮藏28 d期间呈下降趋势,在贮藏63 d前呈相对稳定趋势;肉豆蔻素、榄香素和黄樟素的活化能为2.21 kJ/mol。K为2.71 kJ/mol。和3.22 kJ/mol。分别K。
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引用次数: 0
Extract Corn Silk with Variation of Solvents on Yield, Total Phenolics, Total Flavonoids and Antioxidant Activity 不同溶剂提取玉米丝对产量、总酚、总黄酮及抗氧化活性的影响
Pub Date : 2017-08-31 DOI: 10.22146/ifnp.24280
H. Haslina, Murtiari Eva
Corn silk is a by product or waste that is commonly used as a traditional medicine that contain bioactive compounds such as volatile oils, steroids, alkaloids, tannins, flavonoids, chlorogenic acid and phenolic compounds, containing protein, carbohydrates, minerals (Ca, Mg, Cu , Zn, Fe and Mn), higher crude fiber, vitamin B, vitamin C, vitamin K, steroids such as sitosterol and stigmasterol, alkaloids, saponins, tannins, flavonoids, anthocyanins, protokatekin, vanilic acid, derivatives hasperidin and quersetin, phenols, terpenoids and glycosides, maysin, β-carotene, beta-sitosterol, geraniol, hordenin, limonene, menthol and viteskin also a good source of poliphenol as antioksidan. Solvent used for the extraction of flavonoid compounds are methanol, ethanol, acetone and ethyl acetate. Basic separation by solvent extraction is the difference in the solubility of each composition in compaction with solvent. Solubility is influenced temperature, stirring speed, the extraction time, comprehensive tangent plane solids by solvent extraction and frequency. This study aims to extract corn silk by using a variation of solvent type in terms of yield, total phenols, flavonoids and antioxidant activity. The experimental design used in this study is completely randomized design (CRD) 1 factor with 5 replications. Solvent: methanol: water (85:15) (v/v) ethanol: water (85:15) (v/ v), ethyl acetate: water (85:15) (v/v), acetone: water (85 : 15) (v/v). Furthermore, the data obtained were analyzed variants and if there is a difference between treatments continued by Duncan Multiple Range Test (DMRT) at 5% level. Variations in the type of solvent significant (p <0.05) to the yield, total phenol, total flavonoids and antioxidant activity. Variations in the type of solvent with the highest yield at a ratio (methanol: water), and the ratio (ethanol: water), then the ratio (acetone: water) and the lowest ratio of (ethyl acetate: water). At ratio (methanol: water) obtained yield is 65.8%, total phenol ie 45350.27 mg / kg GAE, total flavonoids 291.28 mg QE / kg and activities of antioxidant that is 92.1%.
玉米丝是一种副产品或废物,通常用作传统药物,含有生物活性化合物,如挥发油、类固醇、生物碱、单宁、类黄酮、绿原酸和酚类化合物,含有蛋白质、碳水化合物、矿物质(Ca、Mg、Cu、Zn、Fe和Mn)、高级粗纤维、维生素B、维生素C、维生素K、类固醇如谷甾醇和豆甾醇,生物碱、皂苷、单宁、黄酮类化合物、花青素、原katekin、香草酸、衍生物橙皮苷和槲皮素、酚类、萜类和糖苷、五月红素、β-胡萝卜素、β谷甾醇、香叶醇、荷兰素、柠檬烯、薄荷醇和卵黄皮也是多酚的良好来源。用于提取类黄酮化合物的溶剂是甲醇、乙醇、丙酮和乙酸乙酯。通过溶剂萃取的基本分离是每种组合物在用溶剂压实时的溶解度差异。溶解度受温度、搅拌速度、萃取时间、溶剂萃取的综合切平面固体和频率的影响。本研究旨在通过使用不同溶剂类型在产量、总酚、黄酮类化合物和抗氧化活性方面提取玉米丝。本研究中使用的实验设计是完全随机设计(CRD)1因子,5次重复。溶剂:甲醇:水(85:15)(v/v)乙醇:水。此外,对所获得的数据进行了变异分析,以及在5%水平下,邓肯多重范围试验(DMRT)继续进行的治疗之间是否存在差异。溶剂类型的变化对产率、总酚、总黄酮和抗氧化活性具有显著性(p<0.05)。在一定比例(甲醇:水)下产率最高的溶剂类型的变化,以及该比例(乙醇:水),然后是该比例(丙酮:水)和最低比例(乙酸乙酯:水)。按比例(甲醇∶水)得到的产率为65.8%,总酚即45350.27mg/kg GAE,总黄酮291.28mg QE/kg,抗氧化活性为92.1%。
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引用次数: 8
Determination of Antioxidant Activity and Phenolic Compounds of Methanolic Extract of Java Plum (Syzygium cumini Linn. (Skeel) Seed 爪哇梅甲醇提取物抗氧化活性及酚类化合物的测定。(斯基尔)种子
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24279
R. Rohadi, U. Santoso, S. Raharjo, I. Falah
Methanolic extract of Java Plum ( Syzygium cumini L. (Skeel) seed (MEJS) is potential source of natural antioxidant. As indicated by several in vitro measurements, the extract had strong DPPH (1,1 diphenyl, 2–picryl hydrazyl) and ABTS (2,2-azinobis, 3-ethylbenzothiazoline-6-sulphonate) radical scavenging activity, strong Ferric Reducing Antioxidant Power (FRAP) and moderate inhibition activity of linoleic acid oxidation. This study aimed to determine antioxidant activity and phenolic compound of Java Plum seed ( Syzygium cumini L. (Skeel) methanolic extract fractions. Phenolics compound identification using Thin Layer Chromatography (TLC) showed that all fractions (polar, semi polar and hydrolyzed semi polar fraction) contained Gallic acid, Tannic acid and flavonol’s Rutin. HPLC-DAD analysis showed that its polar fraction contained 25 ppm flavonol’s Quercetine and 55181 ppm flavonol’s (+)- Catechin, ethyl acetate fraction contained 54 ppm flavonol’s Rutin and 528 ppm (+)- Catechine, while hydrolyzed ethyl acetate fraction contained 404 ppm Rutin and 28692 ppm (+)- Catechine.
爪哇梅(Syzygium cumini L.(Skeel)籽(MEJS)的甲醇提取物是天然抗氧化剂的潜在来源。几项体外测定表明,该提取物具有较强的DPPH(1,1-二苯,2-苦基肼基)和ABTS(2,2-锌二苯,3-乙基苯并噻唑啉-6-磺酸酯)自由基清除活性、较强的铁还原抗氧化能力(FRAP)和中等的亚油酸氧化抑制活性。本研究旨在测定Java Plum seed(Syzygium cumini L.(Skeel)甲醇提取物的抗氧化活性和酚类化合物。薄层色谱法(TLC)对酚类化合物的鉴定表明,所有组分(极性、半极性和水解半极性组分)均含有没食子酸、单宁酸和黄酮醇芦丁。HPLC-DAD分析表明,其极性部分含有25ppm黄酮醇槲皮素和55181ppm黄酮醇(+)-儿茶素,乙酸乙酯部分含有54ppm黄酮醇芦丁和528ppm(+)-Catechine,而水解乙酸乙酯部分包含404ppm芦丁和28692ppm(+。
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引用次数: 8
Pigment Production of Monascus sp. Isolated from Angkak in Semarang Region, Central Java, Indonesia 印度尼西亚中爪哇三宝垄地区Angkak红曲霉分离物的色素生产
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24256
E. Kusdiyantini, S. Nugraha, A. T. Lunggani
Angkak (red fungi rice, red yeast rice) is a traditional fermentation by fungi Monascus in a lot of Asia Countries, which is useful as a natural food colorant.  The aim of this research was to obtain pure isolate of red fungus from angkak in Semarang. The growth and the red pigment production of the selected isolate in the different initial medium pH and nitrogen sources were observed. The treatment was done by growing the isolate in PDB (Potato Dextrose Broth) medium at initial pH 3, 5, 7 and 9 and as well as optimization of nitrogen sources with 1% ammonium chloride, 1% ammonium nitrate, and 1% peptone. Analysis of the fungi growth was performed by weighing mycelia biomass using dry weight method and the red pigments were analysis using spectrophotometer at a wavelength (λ max ) of 500 nm. The results showed that the highest absorbance value of pigment (0.81) was obtained at pH 7, and the highest value of the cell dry weight was also obtained at pH 7 (1.23 g/L). The results showed that 1% ammonium chloride was the best nitrogen source for absorbance of red pigment production (0.82), but the highest of dry weight (2.56 g/L) was obtained by using medium with 1% ammonium nitrate as a nitrogen source.
红曲米(Angkak)是亚洲许多国家传统的红曲霉发酵方法,是一种有用的天然食品色素。本研究的目的是从三宝垄的安家乐中分离纯化红真菌。观察了所选菌株在不同初始培养基pH和不同氮源条件下的生长和红色素产量。在初始pH为3、5、7和9的PDB(马铃薯葡萄糖肉汤)培养基中培养分离物,并以1%氯化铵、1%硝酸铵和1%蛋白胨为氮源进行优化。用干重法测定菌丝体生物量,用分光光度计在波长(λ max)为500 nm的波段对红色素进行分析。结果表明,pH为7时色素吸光度最高(0.81),细胞干重在pH为7时也最高(1.23 g/L)。结果表明,1%的氯化铵是红色素生产吸光度的最佳氮源(0.82),但以1%硝酸铵为氮源的培养基,其干重最高(2.56 g/L)。
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引用次数: 0
Kempong, A Traditional Fermented Food in Karangpucung Kidul village, Linggapura Bumiayu, Central Java: Fermentation Agent and Their Roles 中爪哇Linggapura Bumiayu Karangpucung Kidul村的传统发酵食品Kempong:发酵剂及其作用
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24257
I. Rukmi, Devia Kusmawati Arfina, E. Kusdiyantini
Kempong is a traditional fermented food that is found only in South Karangpucung    Linggapura Bumiayu village, Central Java. It is prepared from palm kernel cake (PKC).  This fermented food is consumed mostly everyday by the people in the village as a side dish or snack. Study on the mold important in the kempong fermentation was done by isolating molds from kempong, PKC, laru and the air of preparation room. Eleven species of molds were successfully isolated from different samples. Among the mold isolates three species came out from kempong product, i.e. R. oryzae, E. chevaliery and A. oryzae . The isolates showed the capability in producing amylase, protease, lipase, and cellulase. Both R. oryzae and A. oryzae indicated as the main fermentation mold in kempong production, because the inoculum laru only contained these two species.
Kempong是一种传统的发酵食品,只有在中爪哇的South Karangpucung Linggapura Bumiayu村才能找到。它是由棕榈仁饼(PKC)制成的。这种发酵食品大多是村里的人每天食用的配菜或小吃。通过对锦锦蓟、锦锦蓟、乳香和制品室空气中霉菌的分离,对锦锦蓟发酵过程中重要的霉菌进行了研究。从不同样品中成功分离出11种霉菌。其中3个菌种为稻谷霉、骑士霉和稻谷霉。该菌株具有生产淀粉酶、蛋白酶、脂肪酶和纤维素酶的能力。由于接种物中只含有米曲霉和米曲霉两种菌种,这两种菌种被认为是甘蓬生产中的主要发酵菌种。
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引用次数: 0
Production and Application of Lactobacillus plantarum IBL-2 Bacteriocins as Meat Product Biopreservatives 肉制品生物防腐剂植物乳杆菌IBL-2细菌素的生产与应用
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24278
M. S. Wibowo, Isra Muzakiyah, B. Nurhayati, T. A. Padmasawitri, Y. Widiastuti, T. Gusdinar
Biopreservation is one of the alternatives to obtain safe food products. The produced bacteriocin by Lactic Acid Bacteria (LAB) is potential as biopreservatives, which is safe for consumption, since it was a protein degradable by proteolytic enzymes. This study aimed to optimize bacteriocin production from L. plantarum IBL-2 and to evaluate the effectiveness of bacteriocins in reducing the number of total plate count and Salmonella typhimurium in ground beef. Bacteriocin was produced through fermentation of L. plantarum IBL-2 , under various conditions to yield the compound with the best antimicrobial activity. The total number of bacteria in ground beef after the addition of L. plantarum IBL-2 fermentation supernatant was determined. The result was compared with the sample without preservatives (control), and sample added with commercial Nissin. All three samples of ground beef were spiked with S. typhimurium and incubated for 0, 2, 6, 8, 12, 14 days at a temperature of 4-10 ° C. Total Plate Count (TPC) method was utilized to determine the number of bacteria in the samples. The fermentation process resulted in bacteriocin with the strongest antimicrobial activity when using low molecular weight liquid medium (LMWLM), followed by a series of refining process. From day 0-14, the number of S. typhimurium, in sample added with L. plantarum IBL-2 fermentation supernatant, was lower than control and sample added with Nissin. The most optimal antimicrobial activity of bacteriocin was obtained using LMWLM as fermentation media, and using a series of refining process consist of bacteriocin supernatant evaporation, membrane ultrafiltration, and gradual fractionation using 80% ammonium sulphate. Bacteriocin from L. plantarum IBL-2 showed antimicrobial activity against S. typhimurium.
生物保存是获得安全食品的替代方法之一。乳酸菌产生的细菌素是一种可被蛋白水解酶降解的蛋白质,具有作为生物防腐剂的潜力,可安全食用。本研究旨在优化植物乳杆菌IBL-2的细菌素产量,并评价细菌素对降低牛肉中鼠伤寒沙门菌总数和鼠伤寒沙门菌的效果。利用植物乳杆菌IBL-2发酵生产细菌素,在不同条件下得到抑菌活性最好的化合物。测定了添加植物乳杆菌IBL-2发酵上清液后牛肉碎的细菌总数。结果与未添加防腐剂的样品(对照)和添加日清的样品进行了比较。在3份牛肉碎样品中加入鼠伤寒沙门氏菌,在4-10℃的温度下培养0、2、6、8、12、14 d,采用总平板计数法(Total Plate Count, TPC)测定样品中的细菌数量。采用低分子量液体培养基(LMWLM)发酵得到抑菌活性最强的细菌素,并进行一系列精制工艺。从第0 ~ 14天开始,添加L. plantarum IBL-2发酵上清液的样品鼠伤寒沙门氏菌数量低于对照组和添加日清的样品。以LMWLM为发酵培养基,通过细菌素上清蒸发、膜超滤、80%硫酸铵逐步分馏等一系列精制工艺,获得了最佳的抑菌活性。植物L. plantarum IBL-2细菌素对鼠伤寒沙门氏菌具有抑菌活性。
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引用次数: 1
Potential of Liquid Smoke from Palm Kernel Shell as Biopreservative to Tuna (Thunnus sp) Fish Protein 棕榈仁壳液烟作为金枪鱼(Thunnus sp)鱼蛋白生物防腐剂的潜力
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24281
M. Lasindrang
The purpose of this research was to evaluate the role of liquid smoke of oil palm kernel as “ biopreservative” on the fish during storage toward chemistry/biochemical or microbiological change at to tuna (thunnus sp) fish.This research was carried out in three steps that is: 1) Making liquid smoke with temperature pirolysis 400 0 C during 90 minutes and liquid smoke fractionation in rank: F1 : liquid temperature 100 0 C; F2: 101 - 125 0 C; F3: 126 – 150 0 C; F4: 151 – 200 0 C. 2) Proximate analysis of liquid smoke FO and Fraction F2 to  tuna fish that analysed with TVB, Total Bacterium, total protein content . The result indicates that the phenol and acid content was more increase with higher fractionation temperature while the carbonyl content was more reduced with higher fractionation temperature.  The TVB value for Tuna fish muscle without soaking higher liquid smoke (38.93 mg N/100g and 36.27 mg N/100g) compared to tuna fish muscle of liquid smoke soaking (F0) that is 13.87 mg N/100g and 11.73 mg N/100g and also soaking with F2 fraction muscle tuna 12.27 mg N/100g.  Total bacterium (2.67 x 10 9 cfu/g and 2.10 x 10 9 cfu / g) compare without soaking liquid smoke (F0) and F2 fraction that is (8.43 x 10 5 cfu/g and 6.23 x 10 5 cfu /g) and (8.57 x 10 5 cfu/g and 6.50 x 10 5 cfu/g).
本研究的目的是评价油棕榈仁液烟作为“生物防腐剂”在金枪鱼贮藏过程中对其化学/生化或微生物变化的影响。本研究分三个步骤进行,即:1)在90分钟内制备温度为400 0 C的液烟,液烟分级:F1:液温为100 0 C;F2: 101 - 125℃;F3: 126 - 150℃;2)用TVB、总细菌、总蛋白含量分析金枪鱼的液烟FO和F2组分的近似分析。结果表明,分馏温度越高,苯酚和酸含量越高,羰基含量越低。未浸泡液体烟的金枪鱼肌肉的TVB值(38.93 mg N/100g和36.27 mg N/100g)高于浸泡液体烟的金枪鱼肌肉(F0) (13.87 mg N/100g和11.73 mg N/100g)和浸泡F2部分肌肉的金枪鱼肌肉(12.27 mg N/100g)。总细菌数分别为2.67 × 109cfu /g和2.10 × 109cfu /g,而未浸泡液烟(F0)和F2分数分别为8.43 × 105cfu /g和6.23 × 105cfu /g和8.57 × 105cfu /g和6.50 × 105cfu /g。
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引用次数: 7
Fatty Acid Composition and Physiochemical Properties in Germinated Black Rice 发芽黑米的脂肪酸组成及理化性质
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24259
Siska Indriarsih, M. Astuti, Sri Kanoni
The objective of the research was to determine the effect of the germination of black rice. Black rice was soaked for 12 h and further germinated for 12, 24, 36, and 48 h. Non-germinated black rice was used as a control. Black rice was germinated for 19-20 h. Fat, and moisture content, total sugar, cooking quality, sensory analysis, and fatty acid composition of germinated black rice were further evaluated. Results show that fat content decreased during germination, but moisture content and total sugar increased. Physiochemical properties, such as length expansion ratio, volumetric expansion, water absorption, gel consistency, and alkali values were in the ranges of 0.77-0.91 mm, 2.42-3.38, 1.64-2.04, 66-82.5 mm, and 2-3.5, respectively. From sensory analysis, most of panelists were favorable for the germinated black rice at 24 h germination. SFA and MUFA tended to decrease during germination but PUFA tended to increase. Thus the germination is effective to improve nutritional values of black rice
本研究的目的是确定黑米发芽的影响。将黑米浸泡12小时,并进一步发芽12、24、36和48小时。使用未发芽的黑米作为对照。黑米发芽19-20小时。对发芽黑米的脂肪、水分含量、总糖、烹饪质量、感官分析和脂肪酸组成进行了进一步评估。结果表明,发芽过程中脂肪含量下降,但水分含量和总糖含量增加。理化性质,如长度膨胀比、体积膨胀、吸水性、凝胶稠度和碱值分别在0.77-0.91mm、2.42-3.38、1.64-2.04、66-82.5mm和2-3.5的范围内。从感官分析来看,大多数小组成员对发芽24小时的黑米是有利的。SFA和MUFA在发芽过程中趋于减少,而PUFA则趋于增加。因此发芽对提高黑米的营养价值是有效的
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引用次数: 1
Binding of Aflatoxin B1 to Lactobacillus paracasei SNP-2 and Stability of Bacteria-AFB1 Complex 黄曲霉毒素B1与副干酪乳杆菌SNP-2的结合及细菌-AFB1复合物的稳定性
Pub Date : 2017-08-31 DOI: 10.22146/IFNP.24258
R. Utami, T. Utami, Suparmo Suparmo, E. Rahayu
The aim of this research was to study the binding ability of viable and non-viable of Lactobacillus paracasei SNP-2 to aflatoxin B 1 (AFB 1 ) in phosphate buffer saline (PBS) at pH 7.3. Bacterial cells were grown in MRS broth at 37 °C for 24 h, and then centrifuged at 1,800 g[a1]  for 20 min at 10 °C to get the pellet. Pellet was suspended in PBS pH 7.3 until the cell concentration was about 10 10 CFU/ml. Viable cells, the heated, and acid-killed cells were evaluated their ability to bind AFB 1 in PBS pH 7.3. Stability of the L. paracasei SNP-2/AFB 1 complexes was evaluated by determining the amount of the released AFB 1 to the PBS following five times washing. The results showed that AFB 1 binding ability to heat-and acid-kill bacteria were higher than that of by viable cells. More than 70% of bound AFB 1 was released from viable bacteria after five times washing. However, the heated and acid-killed cell treatments significantly increased the complex stability of bacteria-AFB 1
本研究的目的是研究在pH 7.3的磷酸盐缓冲盐水(PBS)中,副干酪乳杆菌SNP-2的活菌和不活菌与黄曲霉毒素B1(AFB1)的结合能力。细菌细胞在37°C的MRS肉汤中生长24小时,然后在10°C下以1800 g[a1]离心20分钟以获得颗粒。将颗粒悬浮在pH 7.3的PBS中,直到细胞浓度为约10 10 CFU/ml。评估活细胞、加热的和酸杀死的细胞在PBS pH 7.3中结合AFB1的能力。通过测定洗涤五次后释放到PBS中的AFB1的量来评估副干酪乳杆菌SNP-2/AFB 1复合物的稳定性。结果表明,AFB1对高温和酸性细菌的结合能力高于活细胞。经5次洗涤后,70%以上的结合AFB1从活菌中释放。然而,加热和酸杀死的细胞处理显著增加了细菌AFB1的复杂稳定性
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引用次数: 2
期刊
Indonesian Food and Nutrition Progress
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