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Efficacy of Chinese Medicine on Postoperative Rehabilitation of Non-small Cell Lung Cancer (NSCLC), a Randomized Controlled Study.
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1177/15347354251314529
Yifeng Gu, Yichao Wang, Huiling Zhou, Ao Qi, Guanjin Wu, Jiaqi Li, Congmeng Zhang, Yong Yang, Jialin Yao, Wenxiao Yang, Lingzi Su, Yi Liu, Xueqi Tian, Jiajun Song, Yabin Gong, Xiong Qin, Lijing Jiao, Ling Xu

Background: With an increasing number of non-small cell lung cancer (NSCLC) patients being offered surgical treatment, postoperative rehabilitation is also being increasingly emphasized. Traditional Chinese medicine (TCM) holds promise for enhancing postoperative recovery, with treatment methods still in refinement. This study aims to evaluate the efficacy of Chinese herbal decoction and Liuzijue exercises in NSCLC patients during the rapid postoperative recovery period.

Methods: A randomized, parallel-group clinical trial assigned 50 patients to receive Chinese herbal medicine and Liuzijue exercise plus symptomatic treatment, and 49 to symptomatic treatment alone. Treatment continued until postoperative complications resolved, chest tubes were removed, and no abnormal examination findings were reported. The primary outcome was Quality of Life Questionnare-Core 30 (QLQ-C30) score. Secondary outcomes included MOS item short form 36-Item Short Form Health Survey (SF-36) score, rate of complications, six-minute walk test (6MWT) distance, Leicester Cough Questionnaire (LCQ) score, numerical rating scale (NRS) score, and functional activity score (FAS).

Results: Significant improvements in role (MD: 12.15, 95%CI: 2.99-21.32, P < .05) and social functioning (MD: 10.25, 95%CI: 1.72-18.78, P < .05) were observed in the intervention group vs. controls, as measured by QLQ-C30. The intervention group showed better post-treatment SF-36 scores in Role-Physical (RP), Social Function (SF), Role-Emotional (RE), and Mental Health Summary (MCS) (P < .05). The complication rate was lower in the intervention (20.00%) than the control group (44.44%) (P < .05). The intervention group also had a significant increase in FAS scores (P < .05) and covered a better 6MWT distance (P < .05).

Conclusion: Chinese herbal decoction and Liuzijue exercises during the rapid recovery period after lung cancer surgery enhanced patients' QoL and physical endurance, and mitigated complications.Trial registration: This tial was registered with chictr.org.cn (Registration number:ChiCTR2100044776).

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引用次数: 0
Evaluation of the E-Psycho-Oncological Short-Term Intervention "By Your Side" to Reduce Cancer-Related Distress: A Pilot Study.
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1177/15347354251320047
Sven Erik Strunk, Johanna Graf, Julia Barbara Krakowczyk, Alexandra Wieser, Jana Heinen, Caterina Schug PhD, Lisa Maria Jahre, Eva-Maria Skoda, Yesim Erim, Martin Teufel, Alexander Bäuerle

Objective: A large number of patients with cancer experience distress, but not all of them receive adequate psychological support. The e-psycho-oncological short-term intervention "By your Side" was developed to offer evidence-based and low-threshold support for cancer patients dealing with distress.

Methods: A longitudinal intervention pilot study was conducted from 4 March 2022 to 4 January 2023. N = 91 participants took part in the study. N = 82 completed the first module, while n = 23 successfully completed the whole intervention and were included in the final data analyses. "By your Side" consists of 16 modules based on cognitive behavioral therapy, acceptance and commitment therapy and mindfulness-based stress reduction. Differences in distress between the baseline (T0), post-intervention (T1), and follow-up (T2) were analyzed using an ANCOVA and post-hoc t-tests. Secondary outcomes (anxiety, depression, mindfulness, and self-efficacy) were also compared between measuring points, using ANOVAs and post-hoc tests.

Results: A significant reduction (padj = 0.014, d = 0.654) in distress from baseline (T0, M = 52.68, SD = 16.34) to follow-up (T2, M = 43.84, SD = 17.59) was observed. There was a significant decrease in anxiety symptoms and a significant increase in mindfulness and self-efficacy. The user's satisfaction (M = 28.00, range 8-32) and the usability (M = 89.57, range 0-100) of the intervention were high.

Conclusion: The study provides preliminary evidence that the e-psycho-oncological short-term intervention "By your Side" can successfully reduce the distress of cancer patients. The high dropout rate (74.7%) must be taken into account.

Trial registration: German Clinical Trial Register (https://www.drks.de/search/de/trial/DRKS00036001), DRKS number: 00036001 (retrospectively registered).

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引用次数: 0
Inhibition of Myeloma Cell Function by Cannabinoid-Enriched Product Associated With Regulation of Telomere and TP53 富含大麻素的产品对骨髓瘤细胞功能的抑制与端粒和 TP53 的调节有关
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-09-14 DOI: 10.1177/15347354241267979
Ibrahim Musa, Nan Yang, Joseph Breslin, Orion Paulden, Jan Geliebter, Raj Tiwari, Xiu-Min Li
Multiple myeloma is a hematological cancer caused by the uncontrolled proliferation of abnormal plasma cells in the bone marrow, leading to excessive immunoglobulin production. Our study aimed to examine the anticancer properties of BRF1A, a cannabinoid (CBD)-enriched product, on 2 myeloma cell lines: U266 and ARH-7. We treated U266 and ARH-77 myeloma cells with varying doses of BRF1A and measured the production of IgE and IgG antibodies using ELISA. Cell viability was assessed using trypan blue and CCK-8 assays. We measured the expression of genes related to the production of IgE and IgG antibodies, IgEH, and IgGH. We determined its effect on the expression of telomerase and its phosphorylated form as an indicator of telomere stabilization. Furthermore, we determined its effect on other cancer-related targets such as NF-ĸB, c-Myc, and TP53 in U266 cells using reverse transcription polymerase chain reaction (RT-PCR) and western blotting. BRF1A reduced myeloma cell IgE and IgG production in a time and dose-dependent manner. It also suppressed the expression of p-IκBα, p-NFκB (p65), and total NFκB protein, as well as XBP1u and XBP1s. It increased the gene and protein expression of telomere and hTERT and significantly increased cancer suppressor TP53 gene and p53 protein expression. Additionally, BRF1A decreased the c-Myc gene and protein expression. Our study has shown that a CBD-enriched product can reduce the growth of myeloma cells by suppressing the critical functions of IgE- and IgG-producing cells. This study could help bridge the gap in understanding how cannabinoid-containing products affect cancer, aging, telomere, and cancer-suppressor gene activity.
多发性骨髓瘤是一种血液肿瘤,由骨髓中异常浆细胞失控增殖导致免疫球蛋白产生过多引起。我们的研究旨在考察富含大麻素(CBD)的 BRF1A 对两种骨髓瘤细胞系的抗癌特性:U266和ARH-7。我们用不同剂量的 BRF1A 处理 U266 和 ARH-77 骨髓瘤细胞,并使用 ELISA 检测 IgE 和 IgG 抗体的产生。使用胰蓝和 CCK-8 检测法评估细胞活力。我们测量了与 IgE 和 IgG 抗体、IgEH 和 IgGH 的产生有关的基因的表达。我们测定了它对端粒酶及其磷酸化形式(作为端粒稳定的指标)表达的影响。此外,我们还使用反转录聚合酶链反应(RT-PCR)和免疫印迹法测定了它对其他癌症相关靶点(如 U266 细胞中的 NF-ĸB、c-Myc 和 TP53)的影响。BRF1A 能以时间和剂量依赖的方式减少骨髓瘤细胞 IgE 和 IgG 的产生。它还抑制了 p-IκBα、p-NFκB (p65) 和总 NFκB 蛋白以及 XBP1u 和 XBP1s 的表达。它能增加端粒和 hTERT 的基因和蛋白表达,并显著增加抑癌基因 TP53 和 p53 蛋白的表达。此外,BRF1A 还能降低 c-Myc 基因和蛋白的表达。我们的研究表明,富含 CBD 的产品可以通过抑制 IgE 和 IgG 生成细胞的关键功能来减少骨髓瘤细胞的生长。这项研究有助于填补了解含大麻素产品如何影响癌症、衰老、端粒和抑癌基因活性方面的空白。
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引用次数: 0
Effectiveness of the Traditional Japanese Medicine Goshajinkigan in Preventing Paclitaxel-Induced Peripheral Neuropathy: A Multicenter Randomized Comparative Trial 日本传统药物 Goshajinkigan 对紫杉醇诱发的周围神经病变的预防效果:多中心随机比较试验
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-09-14 DOI: 10.1177/15347354241278635
Yukiko Matsumura, Masayuki Futagami, Tsukasa Baba, Shu Soeda, Hidemichi Watari, Yukihiro Terada, Hideki Tokunaga, Satoru Nagase, Toru Nakanishi, Michiko Kaiho, Yoshihito Yokoyama
Objective:Development of chemotherapy-induced peripheral neuropathy (CIPN) poses significant challenges in cancer treatment, often leading to dose reductions or treatment discontinuation. Goshajinkigan (GJG), a traditional Japanese medicine, has shown promise for alleviating CIPN symptoms. This multicenter, randomized controlled trial aimed to prospectively examine the efficacy of GJG in preventing paclitaxel-induced peripheral neuropathy.Methods:This study enrolled 55 patients with ovarian cancer undergoing first-line chemotherapy using paclitaxel and carboplatin. The participants were randomized into Groups A (GJG initiation after onset of grade 2 neuropathy) and B (prophylactic administration of GJG from 1 week before chemotherapy). The primary endpoints were the proportion with a maximum sensory neuropathy grade and visual analog scale (VAS) scores. The secondary endpoints were the rate of chemotherapy completion and paclitaxel dose reduction due to neurotoxicity.Results:Prophylactic GJG administration (Group B) resulted in significant benefits. While both groups had a similar incidence of grade 2 sensory neuropathy, all patients in Group B with grade 2 neuropathy completed treatment without requiring additional analgesics. Group B exhibited lower VAS scores by the end of the study, reduced reliance on adjuvant analgesics (27.3% vs 66.7% in Group A), and significantly less frequent persistent CIPN 6 months post-chemotherapy (18.2% vs 55.6% in Group A). No differences were observed in the chemotherapy completion rates or CIPN-related changes between the groups.Conclusion:GJG, when administered prophylactically, showed potential for mitigating CIPN symptoms during paclitaxel chemotherapy. While promising, further research with placebo controls and objective measures is essential to comprehensively validate these findings.
目的:化疗诱发的周围神经病变(CIPN)是癌症治疗中的重大挑战,通常会导致剂量减少或治疗中止。Goshajinkigan(GJG)是一种传统的日本药物,在缓解 CIPN 症状方面显示出良好的前景。这项多中心随机对照试验旨在前瞻性地研究 GJG 在预防紫杉醇诱发的周围神经病变方面的疗效。参与者被随机分为A组(出现2级神经病变后开始服用GJG)和B组(化疗前1周开始预防性服用GJG)。主要终点是感觉神经病变达到最高级别的比例和视觉模拟量表(VAS)评分。次要终点是化疗完成率和因神经毒性导致的紫杉醇剂量减少。虽然两组的 2 级感觉神经病变发生率相似,但 B 组所有 2 级神经病变患者都完成了治疗,无需额外使用镇痛药。研究结束时,B 组患者的 VAS 评分较低,对辅助镇痛药的依赖性降低(27.3% 对 A 组的 66.7%),化疗后 6 个月持续性 CIPN 的发生率明显降低(18.2% 对 A 组的 55.6%)。结论:在紫杉醇化疗期间,预防性服用 GJG 有可能减轻 CIPN 症状。虽然前景看好,但要全面验证这些研究结果,还必须进一步开展安慰剂对照和客观测量研究。
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引用次数: 0
Corrigendum to “Pharmacokinetic Analysis of Prognostic Factors in Patients With Advanced-Stage Intrahepatic Cholangiocarcinoma Following the Administration of Capsule Formulation of the Standardized Extract of Atractylodes lancea (Thunb) DC” 对 "晚期肝内胆管癌患者服用白术(Tractylodes lancea (Thunb) DC)标准化提取物胶囊制剂后预后因素的药代动力学分析 "的更正
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-05-03 DOI: 10.1177/15347354241254899
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引用次数: 0
Integrative Nursing Interventions for Cancer-Related Symptoms in Oncology Inpatients: Results of a Descriptive Pilot Study 针对肿瘤住院患者癌症相关症状的综合护理干预:描述性试点研究的结果
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-04-29 DOI: 10.1177/15347354241239930
Lea Raiber, Christian Raff, Johanna Thiele, Klaus Kramer
Background:Integrative nursing (IN) is an essential component of integrative medicine and integrative oncology. IN includes a range of external naturopathic, integrative nursing interventions, such as compresses, embrocation, and foot/hand baths, aimed at alleviating symptoms and side effects of conventional treatment. The project IMPLEMENT-UKU (“Implementation of IN at the University Hospital Ulm”) was accompanied by a descriptive pilot study on the use of IN interventions on cancer-related symptoms in oncology inpatients, the characterization of these patients and the evaluation of the impact.Methods:A single-arm study was conducted using a paper-based questionnaire administered before the IN interventions (t0) and 24 hours after the IN interventions (t1). Topics included sociodemographic data, symptoms, quality of life, health status, psychological burden, attitudes, and experience and satisfaction with the IN interventions. Analyses were descriptive using absolute and relative frequencies.Results:During the 6-month study period out of 66 patients recommended for IN consultation by medical and nursing staff on 2 wards, 62 (93.9%) accepted the offer. Of those patients who received IN, 21 patients (33.9%) participated in the study. The number of IN interventions received per patient ranged from 1 to 3 during the 24-hour survey period. And a total of 36 IN interventions were performed: The most treated body region was the feet/legs (50.0%), followed by the back (25.0%), using oils such as solum oil (41.7%) and lavender oil (13.9%). Embrocation (77.8%) was the predominant type of IN intervention. For patients, the mean FACIT-F score was 29.2 ± 12.5. The mean PHQ-4 score was 3.0 ± 1.9. Regarding sleep quality in the last 4 weeks, 13 participants (61.9%) described it as rather or very poor. Satisfaction with the IN was high, with a large proportion of participants evaluating the IN interventions very positively.Conclusion:The study’s findings suggest that there is a great need for IN among oncology inpatients. These patients are open to and interested in IN interventions and evaluate them positively. IN provides a promising opportunity to provide non-pharmacological support to inpatients. The integration of IN in conventional oncology care settings may enhance patient-centered care and contribute to improved patient wellbeing.
背景:整合护理(IN)是整合医学和整合肿瘤学的重要组成部分。中西医结合护理包括一系列外部自然疗法和中西医结合护理干预措施,如热敷、栓塞、足浴/手浴等,旨在减轻常规治疗的症状和副作用。IMPLEMENT-UKU("在乌尔姆大学医院实施 IN")项目同时还开展了一项描述性试点研究,研究内容包括使用 IN 干预措施治疗肿瘤住院患者的癌症相关症状、这些患者的特征以及对其影响的评估。方法:该研究为单臂研究,在 IN 干预前(t0)和 IN 干预后(t1)24 小时内发放纸质问卷。主题包括社会人口学数据、症状、生活质量、健康状况、心理负担、态度以及对 IN 干预的体验和满意度。结果:在为期 6 个月的研究期间,2 个病房的医护人员建议 66 名患者接受 IN 咨询,其中 62 人(93.9%)接受了咨询。在接受 IN 的患者中,21 名患者(33.9%)参与了研究。在 24 小时的调查期间,每位患者接受 IN 干预的次数从 1 次到 3 次不等。总共进行了 36 次 IN 干预:接受治疗最多的身体部位是足部/腿部(50.0%),其次是背部(25.0%),使用的精油包括苏伦精油(41.7%)和薰衣草精油(13.9%)。栓塞(77.8%)是最主要的 IN 干预方式。患者的平均 FACIT-F 评分为 29.2 ± 12.5。PHQ-4平均得分为3.0 ± 1.9。关于最近 4 周的睡眠质量,13 名参与者(61.9%)认为比较差或非常差。结论:研究结果表明,肿瘤住院患者对心理治疗有很大的需求。这些患者对 IN 干预持开放态度,也很感兴趣,并给予积极评价。心理治疗为住院患者提供非药物支持提供了一个大有可为的机会。在传统的肿瘤治疗环境中融入心理治疗,可以加强以患者为中心的治疗,有助于改善患者的健康状况。
{"title":"Integrative Nursing Interventions for Cancer-Related Symptoms in Oncology Inpatients: Results of a Descriptive Pilot Study","authors":"Lea Raiber, Christian Raff, Johanna Thiele, Klaus Kramer","doi":"10.1177/15347354241239930","DOIUrl":"https://doi.org/10.1177/15347354241239930","url":null,"abstract":"Background:Integrative nursing (IN) is an essential component of integrative medicine and integrative oncology. IN includes a range of external naturopathic, integrative nursing interventions, such as compresses, embrocation, and foot/hand baths, aimed at alleviating symptoms and side effects of conventional treatment. The project IMPLEMENT-UKU (“Implementation of IN at the University Hospital Ulm”) was accompanied by a descriptive pilot study on the use of IN interventions on cancer-related symptoms in oncology inpatients, the characterization of these patients and the evaluation of the impact.Methods:A single-arm study was conducted using a paper-based questionnaire administered before the IN interventions (t0) and 24 hours after the IN interventions (t1). Topics included sociodemographic data, symptoms, quality of life, health status, psychological burden, attitudes, and experience and satisfaction with the IN interventions. Analyses were descriptive using absolute and relative frequencies.Results:During the 6-month study period out of 66 patients recommended for IN consultation by medical and nursing staff on 2 wards, 62 (93.9%) accepted the offer. Of those patients who received IN, 21 patients (33.9%) participated in the study. The number of IN interventions received per patient ranged from 1 to 3 during the 24-hour survey period. And a total of 36 IN interventions were performed: The most treated body region was the feet/legs (50.0%), followed by the back (25.0%), using oils such as solum oil (41.7%) and lavender oil (13.9%). Embrocation (77.8%) was the predominant type of IN intervention. For patients, the mean FACIT-F score was 29.2 ± 12.5. The mean PHQ-4 score was 3.0 ± 1.9. Regarding sleep quality in the last 4 weeks, 13 participants (61.9%) described it as rather or very poor. Satisfaction with the IN was high, with a large proportion of participants evaluating the IN interventions very positively.Conclusion:The study’s findings suggest that there is a great need for IN among oncology inpatients. These patients are open to and interested in IN interventions and evaluate them positively. IN provides a promising opportunity to provide non-pharmacological support to inpatients. The integration of IN in conventional oncology care settings may enhance patient-centered care and contribute to improved patient wellbeing.","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":"40 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140836032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Implementation of a Mindful Walking Intervention in Breast Cancer Patients After Their Primary Oncologic Treatment: Results of a Qualitative Study Within a Randomized Controlled Trial 在乳腺癌患者接受初级肿瘤治疗后对其实施意念步行干预:随机对照试验中的定性研究结果
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-04-24 DOI: 10.1177/15347354241237972
Miriam Ortiz, Maren Luise Schröder, Benno Brinkhaus, Barbara Stöckigt
Background:Breast cancer survivors often suffer from diagnosis- and therapy-related long-term side effects, such as cancer related fatigue, restricted stress resilience and quality of life. Walking as a physical activity and mindfulness practice have been shown to be helpful in studies. The aim of this study was to compare the individual experiences and subjectively perceived effects of walking in combination with mindfulness practice with moderate walking alone in breast cancer patients. This paper focuses on the qualitative results of a mixed-methods pilot study.Methods:Breast cancer patients who had finished their primary oncologic treatment at least 6 months ago were randomized to an 8-week group intervention program of either mindful walking or moderate walking. Within the qualitative study part, semi-structured focus group interviews (2 interviews per study arm) were conducted and analyzed using a qualitative content analysis approach. Audio recorded interviews were transcribed verbatim and pseudonymized. The subsequent data analysis was performed by using MAXQDA®.Results:A total of 51 women (mean age 55.8 [SD 10.9] years) were included in the RCT, among these 20 (mean age 56.7 [SD 12.0] years) participated in the focus group interviews (n = 11 patients of the mindful walking group; n = 9 patients of the walking group). Breast cancer patients in both groups described different effects in the complex areas of self-efficacy, coping, body awareness and self-reflection. While mindful walking primarily promoted body awareness and inner strength by mindfulness in breast cancer patients, moderate walking promoted self-efficacy by a confidence of their body and an easily integrated and accepted way of physical activity.Conclusions:Study interventions and the study setting triggered processes and reflections on one’s own health and situation. However, mindful walking and moderate walking seem to address different resources. This important knowledge may help oncologists and other therapists to assess what type of interventions can best meet the needs and requirements of individual patients.Trial registration:DKRS00011521; prospectively registered 21.12.2016; https://drks.de/search/de/trial/DRKS00011521
背景:乳腺癌幸存者经常会受到与诊断和治疗相关的长期副作用的困扰,如与癌症相关的疲劳、抗压能力受限和生活质量下降。研究表明,步行作为一种体育活动和正念练习是有帮助的。本研究的目的是比较乳腺癌患者在步行与正念练习相结合和单独进行适度步行时的个人体验和主观感受效果。方法:至少在 6 个月前完成初级肿瘤治疗的乳腺癌患者被随机分配到为期 8 周的正念步行或适度步行的小组干预项目中。在定性研究部分,进行了半结构化焦点小组访谈(每个研究臂2次访谈),并采用定性内容分析法进行分析。访谈录音被逐字转录并化名。结果:共有 51 名女性(平均年龄 55.8 [SD 10.9]岁)被纳入 RCT,其中 20 名(平均年龄 56.7 [SD 12.0]岁)参加了焦点小组访谈(n = 11 名正念行走组患者;n = 9 名行走组患者)。两组乳腺癌患者在自我效能感、应对能力、身体意识和自我反思等复杂领域都描述了不同的效果。正念步行主要通过正念促进乳腺癌患者的身体意识和内在力量,而适度步行则通过对自己身体的信心和一种易于整合和接受的体育活动方式来提高自我效能。然而,"用心行走 "和 "适度行走 "似乎解决了不同的资源问题。这一重要知识可能有助于肿瘤学家和其他治疗师评估哪种类型的干预措施最能满足个体患者的需求和要求。试验注册:DKRS00011521;2016年12月21日前瞻性注册;https://drks.de/search/de/trial/DRKS00011521。
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引用次数: 0
Luteolin Inhibits Lung Cancer Cell Migration by Negatively Regulating TWIST1 and MMP2 Through Upregulation of miR-106a-5p 木犀草素通过上调 miR-106a-5p 抑制 TWIST1 和 MMP2,从而抑制肺癌细胞迁移
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-04-22 DOI: 10.1177/15347354241247223
Qiang Wang, Mengyuan Chen, Xiaofang Tang
Background:Luteolin, a common dietary flavonoid found in plants, has been shown to have anti-cancer properties. However, its exact mechanisms of action in non-small cell lung cancer (NSCLC) are still not fully understood, particularly its role in regulating broader genomic networks and specific gene targets. In this study, we aimed to elucidate the role of microRNAs (miRNAs) in NSCLC treated with luteolin, using A549 cells as a model system.Materials and Methods:miRNA profiling was conducted on luteolin-treated A549 cells using Exiqon microarrays, with validation of selected miRNAs by qRT-PCR. Bioinformatic analysis identified the regulatory roles of miRNAs in biological processes and pathways following luteolin treatment. Computational algorithms were employed to identify potential target genes. A549 cells were transfected with miR-106a-5p mimic and inhibitor or their corresponding controls. The expression levels of 2 genes, twist basic helix-loop-helix transcription factor 1 (TWIST1) and matrix metallopeptidase 2 (MMP2), and cell migration were assessed.Results:miRNA profiling identified 341 miRNAs, with 18 exhibiting significantly altered expression ( P < 0.05). Subsequent qRT-PCR analysis confirmed altered expression of 6 selected miRNAs. KEGG and GO analyses revealed significant alterations in pathways and biological processes crucial for tumor biology. TWIST1 and MMP2, which both contain conserved miR-106a-5p binding sites, exhibited an inverse correlation with the expression levels of miR-106a-5p. Dual-luciferase reporter assays confirmed TWIST1 and MMP2 as direct targets of miR-106a-5p. Luteolin treatment led to a reduction in A549 cell migration, and this reduction was further amplified by the overexpression of miR-106a-5p.Conclusion:Luteolin inhibits A549 cell migration by modulating the miRNA landscape, shedding light on its mechanisms and laying the foundation for miRNA-based therapeutic approaches for NSCLC.
背景:叶黄素是植物中常见的膳食类黄酮,已被证明具有抗癌特性。然而,它在非小细胞肺癌(NSCLC)中的确切作用机制仍未完全清楚,尤其是它在调节更广泛的基因组网络和特定基因靶点中的作用。在这项研究中,我们以A549细胞为模型系统,旨在阐明微RNA(miRNA)在经木犀草素处理的NSCLC中的作用。材料与方法:我们使用Exiqon微阵列对经木犀草素处理的A549细胞进行了miRNA分析,并通过qRT-PCR对选定的miRNA进行了验证。生物信息学分析确定了miRNA在木犀草素处理后的生物过程和通路中的调控作用。利用计算算法确定了潜在的靶基因。用 miR-106a-5p mimic 和抑制剂或相应的对照组转染 A549 细胞。结果:miRNA 图谱确定了 341 个 miRNA,其中 18 个的表达发生了显著变化(P < 0.05)。随后的 qRT-PCR 分析证实了 6 个选定 miRNA 表达的改变。KEGG 和 GO 分析表明,对肿瘤生物学至关重要的通路和生物过程发生了重大改变。TWIST1和MMP2都含有保守的miR-106a-5p结合位点,它们与miR-106a-5p的表达水平呈反相关。双荧光素酶报告实验证实 TWIST1 和 MMP2 是 miR-106a-5p 的直接靶标。结论:木犀草素通过调节miRNA格局抑制A549细胞迁移,揭示了其机制并为基于miRNA的NSCLC治疗方法奠定了基础。
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引用次数: 0
Jiedu Xiaozheng Yin Inhibits the Progression of Colitis Associated Colorectal Cancer by Stimulating Macrophage Polarization Towards an M1 Phenotype via the TLR4 Pathway 解毒小柴胡汤通过TLR4途径刺激巨噬细胞向M1表型极化,从而抑制结肠炎相关性结直肠癌的进展
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-04-20 DOI: 10.1177/15347354241247061
Haiqin Liu, Shuo Yan, Ruiming Yang, Caidi Huang, Kangyue Guo, Shi Wang, Yunmei Huang, Dongyi Shen, Ying Lin, Zhiyun Cao, Hangyan Zhong, Jiumao Lin, Xuzheng Chen
To investigate the effect of Jiedu Xiaozheng Yin (JXY) on the polarization of macrophages in colitis-associated colon cancer (CAC). An orthotopic model of CAC was established to monitor changes in the pathological state of mice. Colon length, number of colon tumors were recorded, and indices for liver, spleen, and thymus were calculated. Hematoxylin and eosin (H&E) staining was employed to observe intestinal mucosal injury and tumor formation. Immunohistochemistry (IHC) staining was utilized to investigate the effect of JXY on M1 and M2 polarization of macrophages in the colonic mucosa of CAC mice. For in vitro experiments, RT-qPCR (Reverse Transcription-quantitative PCR) and flow cytometry were used to observe the effect of JXY on various M1-related molecules such as IL-1β, TNF-α, iNOS, CD80, CD86, and its phagocytic function as well as M2-related molecules including Arg-1, CD206, and IL-10. Subsequently, after antagonizing the TLR4 pathway with antagonists (TAK242, PDTC, KG501, SR11302, LY294002), the expression of IL-6, TNF-α, iNOS, and IL-1β mRNA were detected by RT-qPCR. In vivo experiments, the results showed that JXY improved the pathological condition of mice in general. And JXY treatment decreased the shortening of colon length and number of tumors as compared to non-treated CAC mice. Additionally, JXY treatment improved the lesions in the colonic tissue and induced a polarization of intestinal mucosal macrophages towards the M1 phenotype, while inhibiting polarization towards the M2 phenotype. In vitro experiments further confirmed that JXY treatment promoted the activation of macrophages towards the M1 phenotype, leading to increased expression of IL-1β, TNF-α, iNOS, CD80, CD86, as well as enhanced phagocytic function. JXY treatment concomitantly inhibited the expression of M2-phenotype related molecules Arginase-1 (Arg-1), CD206, and IL-10. Furthermore, JXY inhibited M1-related molecules such as IL-6, TNF-α, iNOS, and IL-1β after antagonizing the TLR4 pathway. Obviously, JXY could exhibit inhibitory effects on the development of colon tumors in mice with CAC by promoting M1 polarization through TLR4-mediated signaling and impeding M2 polarization of macrophages.
研究解郁安神汤(JXY)对结肠炎相关性结肠癌(CAC)巨噬细胞极化的影响。建立 CAC 正位模型,监测小鼠病理状态的变化。记录结肠长度、结肠肿瘤数量,并计算肝脏、脾脏和胸腺指数。采用苏木精和伊红(H&E)染色观察肠粘膜损伤和肿瘤形成。免疫组化(IHC)染色用于研究 JXY 对 CAC 小鼠结肠粘膜巨噬细胞 M1 和 M2 极化的影响。在体外实验中,使用 RT-qPCR(逆转录-定量 PCR)和流式细胞术观察 JXY 对各种 M1 相关分子(如 IL-1β、TNF-α、iNOS、CD80、CD86 及其吞噬功能)以及 M2 相关分子(包括 Arg-1、CD206 和 IL-10)的影响。随后,用拮抗剂(TAK242、PDTC、KG501、SR11302、LY294002)拮抗 TLR4 通路后,用 RT-qPCR 检测 IL-6、TNF-α、iNOS 和 IL-1β mRNA 的表达。体内实验结果表明,JXY 可改善小鼠的总体病理状况。与未接受 JXY 治疗的 CAC 小鼠相比,接受 JXY 治疗的小鼠结肠长度缩短,肿瘤数量减少。此外,JXY 还能改善结肠组织的病变,诱导肠粘膜巨噬细胞向 M1 表型极化,同时抑制向 M2 表型极化。体外实验进一步证实,JXY 处理可促进巨噬细胞向 M1 表型活化,导致 IL-1β、TNF-α、iNOS、CD80、CD86 的表达增加,并增强吞噬功能。JXY 处理可同时抑制 M2 型相关分子精氨酸酶-1(Arg-1)、CD206 和 IL-10 的表达。此外,在拮抗 TLR4 通路后,JXY 还能抑制 IL-6、TNF-α、iNOS 和 IL-1β 等 M1 型相关分子的表达。显然,JXY通过TLR4介导的信号传导促进巨噬细胞的M1极化,阻碍巨噬细胞的M2极化,从而对CAC小鼠结肠肿瘤的发展起到抑制作用。
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引用次数: 0
The Effect of Melatonin on Increasing the Health Related Quality of Life in Non-Metastatic Breast Cancer Patients: Three-Year Follow up a Clinical Trial 褪黑素对提高非转移性乳腺癌患者健康相关生活质量的影响:临床试验的三年随访
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-04-16 DOI: 10.1177/15347354241237520
Zahra Keshtpour Amlashi, Fatemeh Sheida, Robab Anbiaee, Leili Tapak, Seyed Rohallah Hosseini, Hossein Mosaedian, Nastaran Barati
Introduction:Although breast cancer is common worldwide, if diagnosed early and treated on time, the probability of recovery is high and patients often experience a long life. Reducing the quality of life is a common side effect in patients. Melatonin may have an important role in fatigue, sleep disorders and, as a result, the health-related quality of life (HRQoL) in people. About 184 patients with breast cancer were enrolled in 2 groups: intervention with daily melatonin intake of 18 mg for 3 years (93 patients) and the control group with placebo intake (91 patients). Health-related quality of life and the effect of melatonin on increasing that were evaluated with the EORTC QLQ-C30 questionnaire, third edition at the beginning, 2 months later and 3 years after the beginning of the study.Results:The general score of the HRQoL was significantly different both in the passage of time and in the comparative study of the 2 groups, and it was better in the melatonin group ( P < .05).Conclusion:Long-term use of 18 mg of melatonin for 3 years in patients with non-metastatic breast cancer can lead to an increase in the patients’ quality of life.
导言:虽然乳腺癌在全世界都很常见,但如果早期诊断并及时治疗,痊愈的概率很高,患者通常都能长寿。降低生活质量是患者常见的副作用。褪黑素可能对疲劳、睡眠障碍,进而对人们的健康相关生活质量(HRQoL)有重要作用。约 184 名乳腺癌患者被分为两组:每天摄入 18 毫克褪黑素的干预组(93 名患者)和摄入安慰剂的对照组(91 名患者),干预组为期 3 年。在研究开始时、2个月后和3年后,使用第三版EORTC QLQ-C30问卷对健康相关生活质量和褪黑素对提高健康相关生活质量的影响进行评估。结果:两组患者的 HRQoL 总分在时间推移和对比研究中均存在显著差异,褪黑素组的 HRQoL 总分更高(P < .05)。结论:非转移性乳腺癌患者长期服用 18 毫克褪黑素 3 年,可提高患者的生活质量。
{"title":"The Effect of Melatonin on Increasing the Health Related Quality of Life in Non-Metastatic Breast Cancer Patients: Three-Year Follow up a Clinical Trial","authors":"Zahra Keshtpour Amlashi, Fatemeh Sheida, Robab Anbiaee, Leili Tapak, Seyed Rohallah Hosseini, Hossein Mosaedian, Nastaran Barati","doi":"10.1177/15347354241237520","DOIUrl":"https://doi.org/10.1177/15347354241237520","url":null,"abstract":"Introduction:Although breast cancer is common worldwide, if diagnosed early and treated on time, the probability of recovery is high and patients often experience a long life. Reducing the quality of life is a common side effect in patients. Melatonin may have an important role in fatigue, sleep disorders and, as a result, the health-related quality of life (HRQoL) in people. About 184 patients with breast cancer were enrolled in 2 groups: intervention with daily melatonin intake of 18 mg for 3 years (93 patients) and the control group with placebo intake (91 patients). Health-related quality of life and the effect of melatonin on increasing that were evaluated with the EORTC QLQ-C30 questionnaire, third edition at the beginning, 2 months later and 3 years after the beginning of the study.Results:The general score of the HRQoL was significantly different both in the passage of time and in the comparative study of the 2 groups, and it was better in the melatonin group ( P &lt; .05).Conclusion:Long-term use of 18 mg of melatonin for 3 years in patients with non-metastatic breast cancer can lead to an increase in the patients’ quality of life.","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":"16 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140611515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Integrative Cancer Therapies
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