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A Qualitative Investigation of Factors Influencing the Integration of Complementary and Integrative Healthcare Recommendations in the Daily Lives of Patients with Cancer. 关于影响将补充和整合医疗建议融入癌症患者日常生活的因素的定性调查。
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-01-01 DOI: 10.1177/15347354241252195
Helena Dürsch, Ursula Boltenhagen, Cornelia Mahler, Stefanie Joos, Szecsenyi Joachim, Nadja Klafke

Background: Many patients diagnosed with cancer use complementary and integrative healthcare (CIH) approaches to manage their cancer- and treatment-related symptoms and improve their well-being. Evidence suggests that counseling on CIH can improve health outcomes and decrease healthcare costs by increasing patient activation. This qualitative study explores the experiences of cancer patients who underwent interprofessional counseling on CIH to gain insights into how these patients were able to integrate recommended CIH measures into their daily lives while undergoing conventional cancer treatment.

Methods: Forty semi-structured interviews were conducted with cancer patients participating in the CCC-Integrativ study and its process evaluation. The interviews were audio-recorded, transcribed verbatim, and analyzed using content analysis following Kuckartz and Rädiker. A purposeful sampling strategy was used to achieve a balanced sample regarding gender, age, cancer diagnosis, and treatment approach.

Results: Most patients with cancer reported largely implementing the CIH recommendations. Participants acknowledged the efficacy of CIH recommendations in managing their symptoms. They felt strengthened and empowered to actively take part in their healthcare decisions. However, the patients encountered obstacles in incorporating the recommended CIH applications into their daily routines. These challenges encompassed the effort required for treatment application (e.g., baths, compresses), limitations imposed by the cancer disease (e.g., fatigue, pain), difficulties acquiring necessary materials, associated costs, and lack of infrastructure for CIH. Facilitators of CIH implementation included the availability of easily manageable CIH measures (e.g., herbal teas), informative materials on their application, distribution of samples, family support, and a high level of self-efficacy. The patient-centered approach and strong patient-provider partnership within the counseling context were perceived as empowering. Participants expressed a desire for a consistent point of contact to address their CIH concerns.

Conclusions: The findings underscore the benefits of CIH counseling for cancer patients' symptom management and overall well-being. Healthcare professionals providing CIH counseling to patients with cancer may recognize the barriers identified to better support their patients in the regular use of CIH.

背景:许多确诊癌症的患者使用补充和综合医疗保健(CIH)方法来控制与癌症和治疗相关的症状,并改善他们的健康状况。有证据表明,CIH 咨询可提高患者的积极性,从而改善健康状况并降低医疗成本。本定性研究探讨了接受跨专业 CIH 咨询的癌症患者的经历,以深入了解这些患者在接受常规癌症治疗的同时如何将建议的 CIH 措施融入日常生活:对参与 CCC-Integrativ 研究及其过程评估的癌症患者进行了 40 次半结构式访谈。对访谈进行了录音、逐字记录,并按照 Kuckartz 和 Rädiker 的方法进行了内容分析。采用了有目的的抽样策略,以实现样本在性别、年龄、癌症诊断和治疗方法方面的平衡:结果:大多数癌症患者表示基本执行了 CIH 建议。参与者认可 CIH 建议在控制症状方面的功效。他们感到自己的力量得到了加强,有能力积极参与医疗决策。然而,患者在将建议的 CIH 应用融入日常生活时遇到了障碍。这些障碍包括:应用治疗方法(如沐浴、热敷)所需的努力、癌症疾病带来的限制(如疲劳、疼痛)、难以获得必要的材料、相关费用以及缺乏 CIH 基础设施。促进 CIH 实施的因素包括易于管理的 CIH 措施(如草药茶)的可用性、有关其应用的信息资料、样本的分发、家庭支持以及高度的自我效能感。在咨询过程中,以患者为中心的方法和患者与医护人员之间牢固的伙伴关系被认为是一种能力。参与者表示希望有一个统一的联络点来解决他们在 CIH 方面的问题:研究结果强调了CIH咨询对癌症患者症状控制和整体健康的益处。为癌症患者提供 CIH 咨询的医护人员可以认识到所发现的障碍,以便更好地支持患者定期使用 CIH。
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引用次数: 0
Harnessing Hyperthermia: Molecular, Cellular, and Immunological Insights for Enhanced Anticancer Therapies. 利用热疗:增强抗癌疗法的分子、细胞和免疫学洞察力》(Molecular, Cellular, and Immunological Insights for Enhanced Anticancer Therapies)。
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-01-01 DOI: 10.1177/15347354241242094
Szilvia Lukácsi, Gyöngyi Munkácsy, Balázs Győrffy

Hyperthermia, the raising of tumor temperature (≥39°C), holds great promise as an adjuvant treatment for cancer therapy. This review focuses on 2 key aspects of hyperthermia: its molecular and cellular effects and its impact on the immune system. Hyperthermia has profound effects on critical biological processes. Increased temperatures inhibit DNA repair enzymes, making cancer cells more sensitive to chemotherapy and radiation. Elevated temperatures also induce cell cycle arrest and trigger apoptotic pathways. Furthermore, hyperthermia modifies the expression of heat shock proteins, which play vital roles in cancer therapy, including enhancing immune responses. Hyperthermic treatments also have a significant impact on the body's immune response against tumors, potentially improving the efficacy of immune checkpoint inhibitors. Mild systemic hyperthermia (39°C-41°C) mimics fever, activating immune cells and raising metabolic rates. Intense heat above 50°C can release tumor antigens, enhancing immune reactions. Using photothermal nanoparticles for targeted heating and drug delivery can also modulate the immune response. Hyperthermia emerges as a cost-effective and well-tolerated adjuvant therapy when integrated with immunotherapy. This comprehensive review serves as a valuable resource for the selection of patient-specific treatments and the guidance of future experimental studies.

热疗,即提高肿瘤温度(≥39°C),作为癌症治疗的一种辅助疗法前景广阔。本综述将重点讨论热疗的两个关键方面:分子和细胞效应及其对免疫系统的影响。热疗对关键的生物过程有深远影响。温度升高会抑制 DNA 修复酶,使癌细胞对化疗和放疗更加敏感。温度升高还会诱导细胞周期停滞并触发细胞凋亡途径。此外,高温还能改变热休克蛋白的表达,而热休克蛋白在癌症治疗中发挥着重要作用,包括增强免疫反应。高热治疗对机体针对肿瘤的免疫反应也有显著影响,有可能提高免疫检查点抑制剂的疗效。轻度全身热疗(39°C-41°C)可模拟发烧,激活免疫细胞并提高新陈代谢率。50°C 以上的强热可释放肿瘤抗原,增强免疫反应。使用光热纳米粒子进行定向加热和给药也可以调节免疫反应。当与免疫疗法相结合时,热疗将成为一种具有成本效益和良好耐受性的辅助疗法。这篇全面的综述为选择针对患者的治疗方法和指导未来的实验研究提供了宝贵的资源。
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引用次数: 0
Corrigendum to "[Thymoquinone and Costunolide Induce Apoptosis of Both Proliferative and Doxorubicin-Induced-Senescent Colon and Breast Cancer Cells]". 对"[胸腺醌和木通内酯诱导增殖性和多柔比星诱导的衰老结肠癌和乳腺癌细胞凋亡]"的更正。
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-01-01 DOI: 10.1177/15347354241290048
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引用次数: 0
Quality Assessment of YouTube Videos on Complementary and Alternative Medicine (CAM) for Cancer Using a Newly Developed Tool. 使用新开发的工具对 YouTube 上有关癌症补充和替代医学 (CAM) 的视频进行质量评估。
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-01-01 DOI: 10.1177/15347354241293417
Sophia Huchel, Alina Grumt, Christian Keinki, Judith Buentzel, Lukas Käsmann, Jutta Huebner

Background: The global burden of cancer continues to rise and complementary and alternative medicine (CAM) is attracting a lot of interest. However, quality of online information on CAM, particularly on platforms like YouTube, remains questionable. This study aimed to create a comprehensive assessment tool to assess the quality of CAM-related YouTube videos, crucial for informed decision-making in oncology.

Methods: The assessment tool was developed by adapting existing criteria for website content analysis to video rating. A YouTube search was conducted using German-language terms related to CAM ("complementary medicine (CM) for cancer" and "alternative medicine (AM) for cancer"). In total 25 videos were assessed based on the defined criteria and assigned to five different types of providers (journalism, healthcare organization, hospital or health insurance, independent person, non-medical organization). Statistical analysis was conducted using IBM SPSS Statistics 27.

Results: Interrater reliability analysis showed an Intraclass Correlation Coefficient (ICC) of .91, indicating good to excellent agreement. The average video result was of poor quality, with none of the videos meeting all criteria. The videos achieved a mean rating of 38.2 points (SD: 6.5 points; possible range: 20-60 points). Journalism-based videos showed the most views per days, particularly surpassing those from hospitals or health insurance providers (Kruskal-Wallis-Test: z = 3.14, P = .02). However, there was no statistically significant correlation between video quality and the type of provider or interaction indices. Videos retrieved under the search term "CM" generally scored higher in quality than those under "AM" (Mann-Whitney U test: U = 39.5, P = .04). Nonetheless, "CM" videos were less frequently viewed (Mann-Whitney U test: U = 31.0, P = .01).

Conclusion: This study, the first of its kind focusing on CAM in cancer care emphasized the challenges in identifying credible sources on social media platforms such as YouTube. The developed assessment tool offers a more detailed evaluation method for health-related videos but requires further refinement and testing. Collaboration between healthcare and media entities is suggested to improve the dissemination of reliable information on platforms like YouTube.

背景:全球癌症发病率持续上升,补充和替代医学(CAM)备受关注。然而,有关 CAM 的在线信息,尤其是 YouTube 等平台上的信息,其质量仍然值得怀疑。本研究旨在创建一个综合评估工具,用于评估与 CAM 相关的 YouTube 视频的质量,这对肿瘤学的知情决策至关重要:评估工具是通过将现有的网站内容分析标准应用于视频评级而开发的。使用与 CAM 相关的德语术语("癌症补充医学 (CM)" 和 "癌症替代医学 (AM)")在 YouTube 上进行搜索。根据所定义的标准共对 25 个视频进行了评估,并将其归入五种不同类型的提供者(新闻、医疗机构、医院或医疗保险、独立人士、非医疗机构)。统计分析使用 IBM SPSS Statistics 27 进行:相互间可靠性分析表明,类内相关系数(ICC)为 0.91,表明一致性良好至极佳。平均视频质量较差,没有一个视频符合所有标准。视频的平均评分为 38.2 分(标准差:6.5 分;可能范围:20-60 分)。新闻类视频的日浏览量最高,尤其超过了医院或医疗保险提供商的视频(Kruskal-Wallis 检验:z = 3.14,P = .02)。然而,视频质量与提供商类型或交互指数之间并无统计学意义上的显著相关性。以 "CM "为搜索关键词检索到的视频质量普遍高于以 "AM "为搜索关键词检索到的视频(曼-惠特尼 U 检验:U = 39.5,P = .04)。然而,"CM "视频的观看次数较少(曼-惠特尼 U 检验:U = 31.0,P = .01):这项研究是首个关注癌症护理中的 CAM 的研究,强调了在 YouTube 等社交媒体平台上识别可信来源的挑战。开发的评估工具为健康相关视频提供了更详细的评估方法,但还需要进一步完善和测试。建议医疗保健机构和媒体实体开展合作,以改善 YouTube 等平台上可靠信息的传播。
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引用次数: 0
The Tanshinones (Tan) Extract From Salvia miltiorrhiza Bunge Induces ROS-Dependent Apoptosis in Pancreatic Cancer via AKT Hyperactivation-Mediated FOXO3/SOD2 Signaling. 丹参提取物通过 AKT 超活化介导的 FOXO3/SOD2 信号诱导胰腺癌发生 ROS 依赖性凋亡
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-01-01 DOI: 10.1177/15347354241258961
Qin Xu, Shujie Dong, Qiuyi Gong, Qun Dai, Rubin Cheng, Yuqing Ge

Context: Salvia miltiorrhiza (SM) is a commonly used herb in traditional Chinese medicine (TCM) and has been used in the treatment of pancreatic cancer to relieve the symptom of "blood stasis and toxin accumulation." Tanshinones (Tan), the main lipophilic constituents extracted from the roots and rhizomes of SM, have been reported to possess anticancer functions in several cancers. But the mechanism of how the active components work in pancreatic cancer still need to be clarified.

Objective: In this study, we aimed to investigate the therapeutic potential of Tan in pancreatic cancer and elucidate the underlying mechanisms.

Materials and methods: The viabilities of PANC-1 and Bxpc-3 cells were determined by MTT assay, after treatment with various concentrations of Tan. The apoptotic cells were quantified by annexin V-FITC/PI staining and DAPI staining assays. The expression of relative proteins was used western blotting. Tumor growth was assessed by subcutaneously inoculating cells into C57BL/6 mice.

Results: Our experiments discovered that Tan effectively suppressed pancreatic cancer cell proliferation and promoted apoptosis. Mechanistically, we propose that Tan enhances intracellular ROS levels by activating the AKT/FOXO3/SOD2 signaling pathway, ultimately leading to apoptosis in pancreatic cancer cells. In vivo assay showed the antitumor effect of Tan.

Conclusion: Tan, a natural compound from Salvia miltiorrhiza, was found to effectively suppress pancreatic cancer cell proliferation and promote apoptosis both in vitro and in vivo. Mechanistically, we propose a positive feedback loop mechanism. These findings provide valuable insights into the molecular pathways driving pancreatic cancer progression.

背景:丹参(Salvia miltiorrhiza,SM)是传统中医学(TCM)中的一种常用草药,被用于治疗胰腺癌,以缓解 "血瘀毒积 "症状。丹参酮(Tanshinones,Tan)是从丹参的根和根茎中提取的主要亲脂成分,据报道对多种癌症具有抗癌作用。但这些活性成分在胰腺癌中的作用机制仍有待明确:在本研究中,我们旨在探讨谭氏菌对胰腺癌的治疗潜力,并阐明其潜在机制:用不同浓度的 Tan 处理 PANC-1 和 Bxpc-3 细胞后,用 MTT 法测定其活力。采用附件素 V-FITC/PI 染色法和 DAPI 染色法对凋亡细胞进行定量。相对蛋白的表达采用了 Western 印迹法。通过将细胞皮下注射到 C57BL/6 小鼠体内来评估肿瘤的生长情况:结果:我们的实验发现,Tan 能有效抑制胰腺癌细胞的增殖并促进其凋亡。从机理上讲,我们认为谭氏疗法通过激活 AKT/FOXO3/SOD2 信号通路来提高细胞内 ROS 水平,最终导致胰腺癌细胞凋亡。体内试验显示了丹参的抗肿瘤作用:结论:从丹参中提取的天然化合物丹参素能有效抑制胰腺癌细胞的体外和体内增殖并促进其凋亡。从机理上讲,我们提出了一种正反馈循环机制。这些发现为研究胰腺癌进展的分子途径提供了有价值的见解。
{"title":"The Tanshinones (Tan) Extract From <i>Salvia miltiorrhiza</i> Bunge Induces ROS-Dependent Apoptosis in Pancreatic Cancer via AKT Hyperactivation-Mediated FOXO3/SOD2 Signaling.","authors":"Qin Xu, Shujie Dong, Qiuyi Gong, Qun Dai, Rubin Cheng, Yuqing Ge","doi":"10.1177/15347354241258961","DOIUrl":"10.1177/15347354241258961","url":null,"abstract":"<p><strong>Context: </strong><i>Salvia miltiorrhiza</i> (SM) is a commonly used herb in traditional Chinese medicine (TCM) and has been used in the treatment of pancreatic cancer to relieve the symptom of \"blood stasis and toxin accumulation.\" Tanshinones (Tan), the main lipophilic constituents extracted from the roots and rhizomes of SM, have been reported to possess anticancer functions in several cancers. But the mechanism of how the active components work in pancreatic cancer still need to be clarified.</p><p><strong>Objective: </strong>In this study, we aimed to investigate the therapeutic potential of Tan in pancreatic cancer and elucidate the underlying mechanisms.</p><p><strong>Materials and methods: </strong>The viabilities of PANC-1 and Bxpc-3 cells were determined by MTT assay, after treatment with various concentrations of Tan. The apoptotic cells were quantified by annexin V-FITC/PI staining and DAPI staining assays. The expression of relative proteins was used western blotting. Tumor growth was assessed by subcutaneously inoculating cells into C57BL/6 mice.</p><p><strong>Results: </strong>Our experiments discovered that Tan effectively suppressed pancreatic cancer cell proliferation and promoted apoptosis. Mechanistically, we propose that Tan enhances intracellular ROS levels by activating the AKT/FOXO3/SOD2 signaling pathway, ultimately leading to apoptosis in pancreatic cancer cells. In vivo assay showed the antitumor effect of Tan.</p><p><strong>Conclusion: </strong>Tan, a natural compound from <i>Salvia miltiorrhiza</i>, was found to effectively suppress pancreatic cancer cell proliferation and promote apoptosis both in vitro and in vivo. Mechanistically, we propose a positive feedback loop mechanism. These findings provide valuable insights into the molecular pathways driving pancreatic cancer progression.</p>","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":"23 ","pages":"15347354241258961"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11191618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluating the Feasibility and Acceptability of a Psychosexual Intervention for Couples Post-allogeneic Hematopoietic Stem Cell Transplantation. 评估针对异体造血干细胞移植后夫妇的性心理干预的可行性和可接受性。
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-01-01 DOI: 10.1177/15347354241230956
Brindha Pillay, David Ritchie, Yvonne Panek-Hudson, Michael Jefford, Teresa Garcia, Cassandra Shields, Jo Gniel, Jo Phipps-Nelson, Allison Drosdowsky, Steve Ellen, Maria Ftanou

Purpose: Allogeneic hematopoietic stem cell transplantation (HSCT) can have a long-term impact on patients' sexual functioning and consequently, their relationship satisfaction. This study aimed to examine the feasibility and acceptability of a 5-session psychosexual intervention for HSCT survivors and their partners.

Methods: To be eligible patients were required to be more than 3 months post-allogeneic HSCT. The intervention comprised 2 components: (1) A psychosexual education session exploring medical/behavioral treatment options for sexual dysfunction, delivered by a specialist nurse; (2) A 4-session Emotionally-Focused Therapy-based relationship education program for couples delivered by a clinical psychologist. Measures assessing relationship quality, sexual functioning, anxiety, and depression were administered pre- and post-intervention. Feasibility was measured via participation rate, adherence and compliance with completing main measures. Acceptability of the intervention was assessed via patient/partner satisfaction surveys.

Results: Eight of 85 (9.4%) eligible patients and their partners participated in the study. Reasons for declining participation included: feeling uncomfortable due to sensitive nature of intervention; did not have any relationship/sexual function issues; and experiencing ongoing medical issues. Six of 8 couples (75%) attended at least 4 intervention sessions and 5 of 8 (62.5%) completed the main study measures post-intervention. Four couples reported that sexual satisfaction and relationship satisfaction was somewhat or much better post-intervention; 1 reported no change.

Conclusion: Feasibility criteria were not met, with low enrolment rate, however the intervention was deemed acceptable. Couples who participated adhered to the intervention and considered it beneficial. Further strategies to identify and manage sexual concerns are required.

目的:异基因造血干细胞移植(HSCT)会对患者的性功能产生长期影响,进而影响他们的关系满意度。本研究旨在探讨对造血干细胞移植幸存者及其伴侣进行为期 5 个疗程的性心理干预的可行性和可接受性:符合条件的患者必须在异基因造血干细胞移植后 3 个月以上。干预包括两个部分:(1)由专科护士提供性心理教育课程,探讨性功能障碍的医学/行为治疗方案;(2)由临床心理学家提供为期 4 个疗程、以情感焦点疗法为基础的夫妻关系教育课程。在干预前后对夫妻关系质量、性功能、焦虑和抑郁进行评估。通过参与率、坚持率和完成主要措施的依从性来衡量可行性。干预的可接受性通过患者/伴侣满意度调查进行评估:85名符合条件的患者及其伴侣中有8人(9.4%)参加了研究。拒绝参与的原因包括:因干预的敏感性而感到不适;没有任何关系/性功能问题;正在经历医疗问题。8 对夫妇中有 6 对(75%)参加了至少 4 次干预课程,8 对夫妇中有 5 对(62.5%)完成了干预后的主要研究措施。四对夫妇表示干预后性生活满意度和夫妻关系满意度有所提高或大幅提高;一对夫妇表示没有变化:结论:由于入选率较低,研究未达到可行性标准,但干预被认为是可以接受的。参加干预的夫妇坚持干预,并认为干预有益。需要进一步的策略来识别和处理性方面的问题。
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引用次数: 0
EXPRESSION OF CONCERN: "Antimetastatic Effects of Celastrus orbiculatus on Human Gastric Adenocarcinoma by Inhibiting Epithelial-Mesenchymal Transition and NF-κB/Snail Signaling Pathway". 表达关切:"通过抑制上皮-间质转化和 NF-κB/Snail 信号通路,黄腹角雉对人类胃腺癌的抗转移作用"。
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-01-01 DOI: 10.1177/15347354241258618
{"title":"EXPRESSION OF CONCERN: \"Antimetastatic Effects of Celastrus orbiculatus on Human Gastric Adenocarcinoma by Inhibiting Epithelial-Mesenchymal Transition and NF-κB/Snail Signaling Pathway\".","authors":"","doi":"10.1177/15347354241258618","DOIUrl":"10.1177/15347354241258618","url":null,"abstract":"","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":"23 ","pages":"15347354241258618"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interdisciplinary Integrative Oncology Group-Based Program: Evaluation of Long-Term Effects on Resilience and Use of Complementary and Alternative Medicine in Patients With Cancer. 跨学科综合肿瘤学小组计划:评估对癌症患者恢复能力和使用辅助与替代医学的长期影响。
IF 2.9 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-01-01 DOI: 10.1177/15347354241269931
Marek Jonas Von Reusner, Bettina Märtens, Stephanie Barthel, Axel Weiser, Yvonne Ziert, Diana Steinmann, Burcu Babadağ-Savaş

Background: Cancer often causes reduced resilience, quality of life (QoL) and poorer overall well-being. To mitigate these problems, complementary and alternative medicine (CAM) is widely used among patients with cancer. This study aimed to evaluate the long-term effects of an interdisciplinary integrative oncology group-based program (IO-GP) on the resilience and use of CAM in patients with cancer.

Methods: This was a prospective, observational, single-center study. Resilience (RS-13), CAM usage (I-CAM-G), QoL (SF-12) and health-related lifestyle factor (nutrition, smoking, alcohol consumption and physical exercise) data were collected for 70 patients who participated in a 10-week IO-GP between January 2019 and June 2022 due to cancer. The IO-GP is offered at the setting of a university hospital and is open to adult patients with cancer. It contains elements from mind-body medicine and positive psychology, as well as recommendations on healthy diet, exercise and CAM approaches. Patients who completed the IO-GP at least 12 months prior (1-4.5 years ago) were included in this study. Statistical analysis included descriptive analysis and parametric and nonparametric tests to identify significant differences (P < .05).

Results: Resilience increased significantly ≥12 months after participation in the IO-GP (n = 44, P = .006, F = 8.274) and had a medium effect size (r = .410). The time since the IO-GP was completed ("12-24 months," "24-36 months," and ">36 months") showed no statistically significant interaction with changes in resilience (P = .226, F = 1.544). The most frequently used CAM modalities within the past 12 months were vitamins/minerals (85.7%), relaxation techniques (54.3%), herbs and plant medicine (41.1%), yoga (41.4%) and meditation (41.4%). The IO-GP was the most common source informing study participants about relaxation techniques (n = 24, 64.9%), meditation (n = 21, 72.4%) and taking vitamin D (n = 16, 40.0%). Significantly greater levels of resilience were found in those practicing meditation (P = .010, d = -.642) or visualization (P = .003, d = -.805) compared to non-practitioners.

Conclusion: IO-GPs have the potential to empower patients with cancer to continue using CAM practices-especially from mind-body medicine-even 1 to 4.5 years after completing the program. Additionally, resilience levels increased. These findings provide notable insight into the long-term effects of integrative oncology interventions on resilience and the use of CAM, especially in patients with breast cancer.

背景:癌症通常会导致复原力下降、生活质量(QoL)降低和整体健康状况不佳。为了缓解这些问题,癌症患者广泛使用补充和替代医学(CAM)。本研究旨在评估跨学科综合肿瘤学小组计划(IO-GP)对癌症患者恢复能力和使用 CAM 的长期影响:这是一项前瞻性、观察性、单中心研究。研究收集了70名癌症患者的复原力(RS-13)、CAM使用情况(I-CAM-G)、QoL(SF-12)和健康相关生活方式因素(营养、吸烟、饮酒和体育锻炼)数据,这些患者在2019年1月至2022年6月期间参加了为期10周的IO-GP。IO-GP 在大学医院的环境中提供,面向成年癌症患者。它包含心身医学和积极心理学的元素,以及关于健康饮食、运动和 CAM 方法的建议。本研究纳入了至少在 12 个月前(1-4.5 年前)完成 IO-GP 的患者。统计分析包括描述性分析以及参数和非参数检验,以确定显著差异(P 结果:参加 IO-GP ≥12 个月后,复原力明显增强(n = 44,P = .006,F = 8.274),且具有中等效应大小(r = .410)。IO-GP完成后的时间("12-24个月"、"24-36个月 "和">36个月")与复原力的变化在统计学上没有显著的交互作用(P = .226,F = 1.544)。过去 12 个月内最常使用的 CAM 方式是维生素/矿物质(85.7%)、放松技巧(54.3%)、草药和植物药(41.1%)、瑜伽(41.4%)和冥想(41.4%)。IO-GP 是向研究参与者介绍放松技巧(24 人,64.9%)、冥想(21 人,72.4%)和服用维生素 D(16 人,40.0%)的最常见来源。与未练习者相比,练习冥想(P = .010,d = -.642)或可视化(P = .003,d = -.805)者的复原力水平明显更高:IO-GPs有可能增强癌症患者继续使用CAM疗法(尤其是身心疗法)的能力,即使是在完成项目1至4.5年后。此外,复原力水平也有所提高。这些发现为综合肿瘤学干预对复原力和使用 CAM(尤其是对乳腺癌患者)的长期影响提供了值得注意的见解。
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引用次数: 0
Anti-proliferative Effects of Pinocembrin Isolated From Anomianthus dulcis on Hepatocellular Carcinoma Cells. 从杜仲中分离出的 Pinocembrin 对肝细胞癌细胞的抗增殖作用
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-01-01 DOI: 10.1177/15347354241237519
Charupong Saengboonmee, Kanyarat Thithuan, Panupong Mahalapbutr, Cheerapinya Taebprakhon, Aamir Aman, Thanyada Rungrotmongkol, Anyanee Kamkaew, Florian Thierry Schevenels, Tanakiat Chompupong, Sopit Wongkham, Ratsami Lekphrom

Background: Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer. Anomianthus dulcis (Dunal) J.Sinclair (syn. Uvaria dulcis) has been used in Thai traditional medicine in various therapeutic indications. Phytochemical constituents of A. dulcis have been isolated and identified. However, their effects on liver cancer and the associated mechanisms have not been elucidated.

Methods: Dry flowers of A. dulcis were extracted using organic solvents, and chromatographic methods were used to purify the secondary metabolites. The chemical structures of the pure compounds were elucidated by analysis of spectroscopic data. Cytotoxicity against HCC cells was examined using SRB assay, and the effects on cell proliferation were determined using flow cytometry. The mechanisms underlying HCC inhibition were examined by molecular docking and verified by Western blot analysis.

Results: Among 3 purified flavonoids, pinocembrin, pinostrobin, and chrysin, and 1 indole alkaloid (3-farnesylindole), only pinocembrin showed inhibitory effects on the proliferation of 2 HCC cell lines, HepG2 and Li-7, whereas chrysin showed specific toxicity to HepG2. Pinocembrin was then selected for further study. Flow cytometric analyses revealed that pinocembrin arrested the HCC cell cycle at the G1 phase with a minimal effect on cell death induction. Pinocembrin exerted the suppression of STAT3, as shown by the molecular docking on STAT3 with a better binding affinity than stattic, a known STAT3 inhibitor. Pinocembrin also suppressed STAT3 phosphorylation at both Tyr705 and Ser727. Cell cycle regulatory proteins under the modulation of STAT3, namely cyclin D1, cyclin E, CDK4, and CDK6, are substantially suppressed in their expression levels.

Conclusion: Pinocembrin extracted from A. dulcis exerted a significant growth inhibition on HCC cells via suppressing STAT3 signaling pathways and its downstream-regulated genes.

背景:肝细胞癌(HCC)是最常见的原发性肝癌。Anomianthus dulcis (Dunal) J.Sinclair(同义词:Uvaria dulcis)在泰国传统医学中被用于各种治疗适应症。A. dulcis 的植物化学成分已被分离和鉴定。然而,它们对肝癌的影响及其相关机制尚未阐明:方法:使用有机溶剂提取 A. dulcis 的干花,并使用色谱法纯化次生代谢物。通过分析光谱数据,阐明了纯化合物的化学结构。利用 SRB 试验检测了对 HCC 细胞的细胞毒性,并利用流式细胞仪测定了对细胞增殖的影响。通过分子对接研究了抑制 HCC 的机制,并通过 Western 印迹分析进行了验证:结果:在3种纯化的黄酮类化合物(pinocembrin、pinostrobin、chrysin)和1种吲哚生物碱(3-farnesylindole)中,只有pinocembrin对HepG2和Li-7两种HCC细胞株的增殖有抑制作用,而chrysin对HepG2有特异性毒性。因此,我们选择了皮诺孕素进行进一步研究。流式细胞计数分析表明,皮诺孕素可将 HCC 细胞周期阻滞在 G1 期,对诱导细胞死亡的影响极小。通过与 STAT3 的分子对接,Pinocembrin 可抑制 STAT3,其结合亲和力优于 STAT3 抑制剂 stattic。Pinocembrin 还能抑制 STAT3 在 Tyr705 和 Ser727 的磷酸化。受 STAT3 调控的细胞周期调节蛋白,即细胞周期蛋白 D1、细胞周期蛋白 E、CDK4 和 CDK6 的表达水平受到了大幅抑制:结论:从A. dulcis中提取的Pinocembrin通过抑制STAT3信号通路及其下游调控基因,对HCC细胞的生长具有显著的抑制作用。
{"title":"Anti-proliferative Effects of Pinocembrin Isolated From <i>Anomianthus dulcis</i> on Hepatocellular Carcinoma Cells.","authors":"Charupong Saengboonmee, Kanyarat Thithuan, Panupong Mahalapbutr, Cheerapinya Taebprakhon, Aamir Aman, Thanyada Rungrotmongkol, Anyanee Kamkaew, Florian Thierry Schevenels, Tanakiat Chompupong, Sopit Wongkham, Ratsami Lekphrom","doi":"10.1177/15347354241237519","DOIUrl":"10.1177/15347354241237519","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer. <i>Anomianthus dulcis</i> (Dunal) J.Sinclair (syn. <i>Uvaria dulcis</i>) has been used in Thai traditional medicine in various therapeutic indications. Phytochemical constituents of <i>A. dulcis</i> have been isolated and identified. However, their effects on liver cancer and the associated mechanisms have not been elucidated.</p><p><strong>Methods: </strong>Dry flowers of <i>A. dulcis</i> were extracted using organic solvents, and chromatographic methods were used to purify the secondary metabolites. The chemical structures of the pure compounds were elucidated by analysis of spectroscopic data. Cytotoxicity against HCC cells was examined using SRB assay, and the effects on cell proliferation were determined using flow cytometry. The mechanisms underlying HCC inhibition were examined by molecular docking and verified by Western blot analysis.</p><p><strong>Results: </strong>Among 3 purified flavonoids, pinocembrin, pinostrobin, and chrysin, and 1 indole alkaloid (3-farnesylindole), only pinocembrin showed inhibitory effects on the proliferation of 2 HCC cell lines, HepG2 and Li-7, whereas chrysin showed specific toxicity to HepG2. Pinocembrin was then selected for further study. Flow cytometric analyses revealed that pinocembrin arrested the HCC cell cycle at the G1 phase with a minimal effect on cell death induction. Pinocembrin exerted the suppression of STAT3, as shown by the molecular docking on STAT3 with a better binding affinity than stattic, a known STAT3 inhibitor. Pinocembrin also suppressed STAT3 phosphorylation at both Tyr705 and Ser727. Cell cycle regulatory proteins under the modulation of STAT3, namely cyclin D1, cyclin E, CDK4, and CDK6, are substantially suppressed in their expression levels.</p><p><strong>Conclusion: </strong>Pinocembrin extracted from <i>A. dulcis</i> exerted a significant growth inhibition on HCC cells <i>via</i> suppressing STAT3 signaling pathways and its downstream-regulated genes.</p>","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":"23 ","pages":"15347354241237519"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140093939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering the Mechanism of Siwu Decoction Inhibiting Liver Metastasis by Integrating Network Pharmacology and In Vivo Experimental Validation. 网络药理学与体内实验验证相结合,破译四物汤抑制肝转移的机制
IF 2.9 3区 医学 Q1 Medicine Pub Date : 2024-01-01 DOI: 10.1177/15347354241236205
Xuelei Chu, Feiyu Xie, Chengzhi Hou, Xin Zhang, Sijia Wang, Hongting Xie, Chen An, Ying Li, Leyi Zhao, Peng Xue, Shijie Zhu

Background: Siwu Decoction (SWD) is a well-known classical TCM formula that has been shown to be effective as a basis for preventing and reducing liver metastases (LM). However, the active ingredients and potential molecular mechanisms remain unclear.

Objective: This study aimed to systematically analyze the active ingredients and potential molecular mechanisms of SWD on LM and validate mechanisms involved.

Materials and methods: The active ingredients in SWD were extracted by UHPLC-MS/MS in a latest study. Protox II was retrieved to obtain toxicological parameters to detect safety. Swiss Target Prediction database was exploited to harvest SWD targets. Five databases, Gene Cards, DisGeNET, Drugbank, OMIM, and TTD, were employed to filter pathogenic targets of LM. STRING database was utilized to construct the protein-protein interaction network for therapeutic targets, followed by Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. GEPIA database and the Human Protein Atlas were taken to observe the expression of core genes and proteins. ImmuCellAI algorithm was applied to analyze the immune microenvironment and survival relevant to core genes. Molecular docking was performed to verify the affinity of SWD effective ingredients to core targets. In vivo experiments were carried out to validate the anti-LM efficacy of SWD and verify the pivotal mechanisms of action.

Results: Eighteen main bioactive phytochemicals identified were all non-hepatotoxic. PPI network acquired 118 therapeutic targets, of which VEGFA, CASP3, STAT3, etc. were identified as core targets. KEGG analysis revealed that HIF-1 pathway and others were critical. After tandem targets and pathways, HIF-1/VEGF was regarded as the greatest potential pathway. VEGFA and HIF-1 were expressed differently in various stages of cancer and normal tissues. There was a negative regulation of immunoreactive cells by VEGFA, which was influential for prognosis. Molecular docking confirmed the tight binding to VEGFA. This study revealed the exact effect of SWD against LM, and identified significant inhibition the expression of HIF-1α, VEGF, and CD31 in the liver microenvironment.

Conclusion: This study clarified the active ingredients of SWD, the therapeutic targets of LM and potential molecular mechanisms. SWD may protect against LM through suppressing HIF-1/VEGF pathway.

背景:四物汤(SWD)是一个著名的经典中医方剂,已被证明可有效预防和减少肝转移(LM)。然而,其有效成分和潜在的分子机制仍不清楚:本研究旨在系统分析社维錠對肝轉移腫瘤的有效成分和潛在分子機制,並驗證其中涉及的機制:在最新研究中,采用超高效液相色谱-质谱/质谱(UHPLC-MS/MS)技术提取了SWD中的活性成分。通过检索 Protox II 获得毒理学参数,以检测其安全性。瑞士靶标预测数据库(Swiss Target Prediction database)被用来获取 SWD 的靶标。利用 Gene Cards、DisGeNET、Drugbank、OMIM 和 TTD 五个数据库筛选 LM 的致病靶标。利用 STRING 数据库构建治疗靶点的蛋白质-蛋白质相互作用网络,然后进行基因本体和京都基因组百科全书的通路富集分析。GEPIA 数据库和人类蛋白质图谱用于观察核心基因和蛋白质的表达。应用 ImmuCellAI 算法分析与核心基因相关的免疫微环境和存活情况。进行分子对接以验证 SWD 有效成分与核心靶点的亲和力。通过体内实验验证了 SWD 的抗 LM 效能,并验证了其关键的作用机制:结果:发现的 18 种主要生物活性植物化学物质均无肝毒性。PPI网络获得了118个治疗靶点,其中VEGFA、CASP3、STAT3等被确定为核心靶点。KEGG 分析显示,HIF-1 通路等是关键靶点。在串联靶点和通路之后,HIF-1/VEGF 被认为是最具潜力的通路。VEGFA 和 HIF-1 在不同阶段的癌症和正常组织中有不同的表达。VEGFA 对免疫活性细胞有负向调节作用,这对预后有影响。分子对接证实了其与 VEGFA 的紧密结合。本研究揭示了 SWD 对 LM 的确切作用,并发现其对肝脏微环境中 HIF-1α、VEGF 和 CD31 的表达有显著抑制作用:本研究阐明了SWD的活性成分、LM的治疗靶点和潜在的分子机制。SWD可通过抑制HIF-1/VEGF途径保护肝癌患者。
{"title":"Deciphering the Mechanism of Siwu Decoction Inhibiting Liver Metastasis by Integrating Network Pharmacology and In Vivo Experimental Validation.","authors":"Xuelei Chu, Feiyu Xie, Chengzhi Hou, Xin Zhang, Sijia Wang, Hongting Xie, Chen An, Ying Li, Leyi Zhao, Peng Xue, Shijie Zhu","doi":"10.1177/15347354241236205","DOIUrl":"10.1177/15347354241236205","url":null,"abstract":"<p><strong>Background: </strong>Siwu Decoction (SWD) is a well-known classical TCM formula that has been shown to be effective as a basis for preventing and reducing liver metastases (LM). However, the active ingredients and potential molecular mechanisms remain unclear.</p><p><strong>Objective: </strong>This study aimed to systematically analyze the active ingredients and potential molecular mechanisms of SWD on LM and validate mechanisms involved.</p><p><strong>Materials and methods: </strong>The active ingredients in SWD were extracted by UHPLC-MS/MS in a latest study. Protox II was retrieved to obtain toxicological parameters to detect safety. Swiss Target Prediction database was exploited to harvest SWD targets. Five databases, Gene Cards, DisGeNET, Drugbank, OMIM, and TTD, were employed to filter pathogenic targets of LM. STRING database was utilized to construct the protein-protein interaction network for therapeutic targets, followed by Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. GEPIA database and the Human Protein Atlas were taken to observe the expression of core genes and proteins. ImmuCellAI algorithm was applied to analyze the immune microenvironment and survival relevant to core genes. Molecular docking was performed to verify the affinity of SWD effective ingredients to core targets. In <i>vivo</i> experiments were carried out to validate the anti-LM efficacy of SWD and verify the pivotal mechanisms of action.</p><p><strong>Results: </strong>Eighteen main bioactive phytochemicals identified were all non-hepatotoxic. PPI network acquired 118 therapeutic targets, of which VEGFA, CASP3, STAT3, etc. were identified as core targets. KEGG analysis revealed that HIF-1 pathway and others were critical. After tandem targets and pathways, HIF-1/VEGF was regarded as the greatest potential pathway. VEGFA and HIF-1 were expressed differently in various stages of cancer and normal tissues. There was a negative regulation of immunoreactive cells by VEGFA, which was influential for prognosis. Molecular docking confirmed the tight binding to VEGFA. This study revealed the exact effect of SWD against LM, and identified significant inhibition the expression of HIF-1α, VEGF, and CD31 in the liver microenvironment.</p><p><strong>Conclusion: </strong>This study clarified the active ingredients of SWD, the therapeutic targets of LM and potential molecular mechanisms. SWD may protect against LM through suppressing HIF-1/VEGF pathway.</p>","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":"23 ","pages":"15347354241236205"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140093940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Integrative Cancer Therapies
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