Integrative Cancer Therapies最新文献

Background: Glioblastoma multiforme (GBM) is an aggressive brain tumor with limited treatment options and poor prognosis. Emerging evidence suggests that integrative oncology approaches may provide survival benefits when combined with conventional treatments. This study examines whether an integrative oncology treatment plan incorporating modulated electro-hyperthermia (mEHT) improves survival in GBM patients.

Methods: This retrospective cohort study analyzed data from GBM patients treated at the Integrated Health Clinic (IHC) between 2010 and 2024. Survival outcomes were compared between IHC patients receiving adjuvant integrative naturopathic therapies and a matched control group from the National Cancer Institute Surveillance, Epidemiology, and End Results (SEER) database. Kaplan-Meier survival estimates, and Cox proportional hazard models were conducted to assess survival differences. Secondary analyses evaluated the impact of treatment timing (≤120 days vs >120 days post-diagnosis) and age on survival.

Results: The integrative treatment cohort demonstrated a lower hazard of mortality than the SEER group (HR = .72, 95% CI: .53-1.00, P-value = .05). The treatment benefit was greater among IHC patients who started treatment within 120 days of diagnosis (HR = .52, 95% CI: .33-.83, P-value = .006) and those under age 50 (HR = .51, 95% CI: .31-.85, P-value = .009).

Conclusions: The findings suggest that an integrative naturopathic approach incorporating mEHT may improve survival outcomes in GBM patients. Patients initiating integrative treatment earlier experienced a greater survival benefit, as did patients under 50 years of age. Further studies, ideally prospective randomized controlled trials, are warranted to validate these findings.

This study investigated the anti-cancer effects of the ethyl acetate fraction from Opuntia humifusa on human breast cancer MDA-MB-231 cells. O. humifusa, a cactus species capable of surviving under extreme environmental conditions, is currently being studied for its potential pharmacological properties. The MDA-MB-231 cell line is a highly invasive and metastatic model of triple-negative breast cancer (TNBC), which lacks estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2. TNBCs are often clinically challenging because they generally have a poor prognosis and limited treatment options. The ethyl acetate fraction was obtained from ethanol extracts of fruit powder of O. humifusa. Treating MDA-MB-231 cells with ethyl acetate fraction for 48 hours reduced cell viability by more than 50% and inhibited migration demonstrated by wound healing assays. DAPI staining proved nuclear condensation and fragmentation, and flow cytometry confirmed the induction and progression of apoptosis and arrest of cell cycle at the S and G2/M phases. Western blot analysis revealed that Akt, ERK1/2, integrin β1, HIF-1α, MMP-9, MMP-7, CDK2, cyclin A2, pro-caspase-9, pro-caspase-8, pro-caspase-3, and lamin A/C were decreased in a dose-dependent manner, whereas Smac/DIABLO, cleaved caspase-9, cleaved caspase-8, and cleaved caspase-3 were increased in a concentration-dependent manner, indicating downregulation of proliferation, activation of upstream signal transduction influencing cell cycle arrest and apoptosis, deactivation of metastasis, induction of cell cycle arrest at the S and G2/M phases, and activation of intrinsic and extrinsic apoptotic pathways. These findings suggest that the ethyl acetate fraction of O. humifusa induces apoptosis, cell cycle arrest, and anti-metastasis as well as contributing to upstream signal transduction of anti-proliferation, apoptosis, and cell cycle arrest in human triple-negative breast cancer cells.

Background: Colorectal cancer (CRC) imposes a heavy disease burden. Besides physical morbidity, some patients might still experience long-term psychological distress. Our previous study demonstrated that physical symptoms and psychological distress of CRC improved following traditional Chinese medicine (TCM) combined online group psychotherapy. However, the multidimensional nature of these experiences warrants deeper exploration of patients' lived perspectives.

Methods: We designed a single-arm phase I clinical trial, in which 40 CRC patients (aged between 18 and 75) who have received radical surgery (stage I-III) were recruited. This 6-week intervention of TCM combined online group psychotherapy included 90 minutes' communication on various topics for each week. The video of each online group psychotherapy session was saved, and the 38 patient's' speech was analyzed by thematic analysis in the qualitative study.

Results: We identified 4 themes and 13 subthemes. The patients mainly displayed foundational outlook transformation and tangible lifestyle reformation. The physical symptoms and psychological symptoms also had a certain degree of relief. In addition, patients also showed an increased demand for medical advice and health care information, which indicates that they were more concerned about their health condition, and the needs of patients were responded to in treatment, resulting in corresponding benefits.

Conclusion: During TCM combined online group psychotherapy interventions-including education, skill-building, TCM lifestyle coaching, and peer-exchange platforms-patients developed sustained self-health management practices, improving psychological resilience and physical symptoms. This holistic, tailored and culturally sensitive approach fostered long-term recovery and independence of CRC survivors.

Background: Breast cancer is the most common cancer and the main cause of death because of malignant tumors in women, worldwide. The impact of Crocus sativus on several cancers has been discussed. Recent studies provide evidence regarding the anticancer properties of C. sativus and its bioactive constituents against breast cancer. This study aims to systematically review the efficacy of this botanical drug and its constituents on breast cancer, and their mechanism of action for the first time. Due to the lack of human studies in this field, the present research focused on preclinical studies.

Methods: In this systematic review, scientific databases, including PubMed, Web of Sciences, Google Scholar, Scopus, and Scientific Information Database were explored profoundly. Preclinical studies published until the end of 2024 that had investigated the therapeutic properties of C. sativus, or its bioactive constituents including crocin, crocetin, safranal, or picrocrocin against breast cancer were selected.

Results: Forty-four studies examining the effect of C. sativus or its bioactive constituents against breast cancer were obtained. Preclinical in vitro and in vivo studies have demonstrated the potential and targeted anticancer properties of the metabolites found in saffron (C. sativus). These metabolites, such as crocin, crocetin, and safranal, exhibit their anticancer effects through various mechanisms, including induction of apoptosis, modulation of the cell cycle, and other pathways.

Conclusions: C. sativus and its constituents exerts their anticancer activity with different mechanisms. A considerable number of the included studies highlight induction of apoptosis and modulation of the cell cycle. The findings of our study suggest that certain compounds, particularly crocin and crocetin, have significant anticancer properties. In particular, crocin provided the highest level of evidence of efficacy in preclinical research, indicating its potential for further investigation.

Background: Cancer-related fatigue is the most common complication in patients. Astragalus membranaceus is widely used in many countries to treat cancer, but its efficacy and safety is uncertain. Objectives: This study aimed to summarize the evidence on Astragalus membranaceus on cancer-related fatigue and quality of life in patients with cancer. Methods: Nine electronic databases were explored for the clinical randomized controlled trial of intervention with Astragalus membranaceus alone for cancer-related fatigue and quality of life in cancer patients from inception to July 1, 2022. The risk of bias assessment tool was adopted by Cochrane Handbook 6.1.0. The effect size was estimated using relative risk and mean difference with a corresponding 95% confidence interval. Review Manager 5.4 was used for meta-analysis. The evidence level was assessed using Grading of Recommendation, Assessment, Development and Evaluation (GRADE). Results: Eight studies were included. The results of the meta-analysis showed that the addition of Astragalus membranaceus to the control group was effective in reducing cancer-related fatigue (SMD = -1.63, 95% CI [-1.90, -1.36], P < .00001) and (RR = 1.55, 95% CI [1.19, 2.02], P = 0.001) in patients with cancer and improving quality of life (SMD = 0.86, 95% CI [0.17, 1.55], P = 0.01) and (RR = 1.57, 95% CI [1.10, 2.23], P = 0.01). Conclusion: The current evidence is supportive of the efficacy of Astragalus membranaceus in patients with cancer-related fatigue and their quality of life, but due to the small and low quality of the included literature and the lack of uniformity in terms of cancer type as well as treatment modalities, there is currently insufficient evidence to provide strong support for the clinical use of Astragalus membranaceus in the treatment of cancer-related fatigue. More high-quality evidence is needed in the future to further validate the use of Astragalus membranaceus in the treatment of clinical cancer-related fatigue. Registration: A review protocol was developed and registered in the International Prospective Register of Systematic Reviews (PROSPERO). Registration number: CRD42023442277. Registered 20 July 2023.

Rationale: Papillary thyroid carcinoma (PTC) is the most common thyroid cancer that typically affects women ages 20 to 50, presenting as an asymptomatic neck mass. Treatment with total or partial thyroidectomy shows an excellent prognosis. However, investigation of non-invasive therapeutic options with minimal adverse effects is ongoing. This study seeks to investigate the K1 cell line, which consists of PTC cells obtained from metastatic tumors of well-differentiated PTC.

Objective: Our investigation focuses on a cannabinoid-based product (named BRF1-A) and its potential anti-cancer effects through modulation of gene expression. We investigated its effects on gene expression of p53, c-Myc, and BCL-2 in K1 papillary thyroid cancer cells.

Methods: BRF1A was co-cultured with K1 cell line (1 × 10⁶ cells/ml) and incubated at 37°C under 5% CO₂ for 24 and 48 hours. After the culture time points, the cells were harvested, and cell viability was determined via trypan blue exclusion assay. Using qRT-PCR, we determined the effect on the gene expression of TP53, c-Myc, and BCL-2.

Results: Results show that the BRF1A decreased the viability of K1 PTC cells in a dose and time-dependent manner. Within 24 hours, the cannabinoid- containing product increased the gene expression of TP53 and decreased the gene expression of BCL-2 and c-Myc in K1 PTC cells.

Conclusion: The results suggest that the cannabinoid-containing product BRF1A interacts as a potential regulator in well-differentiated thyroid cancer with the upregulation of p53 and downregulation of BLC-2 and c-Myc. Further in vitro and in vivo studies are needed to understand the exact mechanism and therapeutic potential of the cannabinoid-containing products in papillary thyroid cancer.

Introduction: Balance problems arising from cancer and its treatments can significantly impact daily functionality and quality of life. Improving balance as part of a cancer treatment plan could result in better patient outcomes. Thus, the aim of this study was to determine whether an integrative therapeutic yoga intervention can improve balance in a heterogenous population of cancer survivors (CS).

Methods: This is a secondary analysis of data from a 16-week feasibility study where therapeutic yoga was supplemented with psychosocial support to maximize health-related quality of life in adult CS of any stage and site. In this study, we investigated balance, as it has been shown to be an important outcome in CS due to its role in physical function and quality of life. The intervention included therapeutic yoga three times per week for 16 weeks and daily psychosocial support provided via text message. Participants' balance was assessed while standing on a pressure mat with feet together, eyes opened and closed, for 30 seconds in each condition. Data on the "sway path distance" (displacement of the center of gravity) in the two conditions were obtained. Changes in balance after the intervention (from baseline to follow-up) were analyzed using paired-sample t-tests. Changes in balance were also assessed using responder analysis. We described the proportion of participants that improved their balance or not based on 10% difference from baseline scores.

Results: Of the 29 participants included, 22 (76%) completed post-assessments. Changes in both balance assessment conditions were not statistically significant (eyes opened: 80.06 ± 374.99, p = .702; eyes closed: -1.82 ± 24.01, p = .068). Responder analysis showed that 8 participants improved their balance with eyes opened, while 8 worsened, and 6 did not change. Analysis of balance with eyes closed showed that 5 improved, 8 worsened, and 9 did not change.

Conclusion: This secondary analysis of data from a heterogenous cohort of adult CS did not support our hypothesis at the group level. However, at the individual level, responder analysis indicated improved balance in some survivors. Future research is needed to determine factors related to the cancer experience which might mediate balance outcomes to inform better integrative interventions.

Background: Numbness and tingling secondary to chemotherapy-induced peripheral neuropathy (CIPN) are frequent side effects that limit chemotherapy treatment and quality of life. Successful treatments for CIPN are limited. This preliminary report shows the potential long-term effects of ozone treatment in the management of persistent numbness and tingling secondary to CIPN.

Methods: Ozone treatment was administered by rectal insufflation in 15 patients (female/male: 8/7, age: 66 years old) suffering from persistent numbness and tingling secondary to grade-2 or grade-3 CIPN. Planned ozone treatment consisted of 40 sessions over 4 months. The initial concentration of 10 μg/mL was progressively increased to 30 μg/mL. The initial gas volume of 180 mL/session was progressively increased to 300 mL/session if tolerated. Before and after ozone treatment, and at 3- and 6- months after the end of treatment, they were assessed (i) the grade of CIPN-toxicity, and (ii) the self-reported decrease in numbness and tingling.

Results: After ozone treatment, 47% of patients experienced a decrease in the grade of CIPN-toxicity (P = .016), and 67% of patients reported a decrease in numbness and tingling ≥50% (P = .002). These effects were maintained at 3- and 6- months after the end of O₃T.

Conclusions: In this retrospective report, patients with persistent numbness and tingling secondary to CIPN showed clinically relevant and long-term improvements after ozone treatment. The magnitude and duration of the observed effects merit further research and support our ongoing clinical trials.

Objective: To observe the clinical efficacy of Dendrobium officinale in the treatment of radiotherapy-induced oral mucositis in nasopharyngeal carcinoma patients, and to explore its regulating effect on immune function and oral microbiota by comparing immune-related factors and oral microbiota before and after the intervention.

Methods: We conducted a randomized double-blinded controlled trial in Zhejiang Cancer Hospital. Sixty patients with nasopharyngeal cancer combined with radiotherapy-induced oral mucositis were randomly divided into a study group and control group, with 30 cases in each group The study group used compound vitamin B12 solution and Dendrobium tea drink, and the control group simply used compound vitamin B12 solution rinse. When the patients developed radiotherapy-induced oral mucositis (at the time of 10F radiotherapy), and after 1 month of Dendrobium treatment (at the end of radiotherapy), the salivary flow rate was measured without stimulation to evaluate the degree of oral mucositis and the clinical efficacy. We also detected the content of EGF in saliva and the content of IL-10 and IL-11 in serum, and analyzed the differences in microbial community structure. All patients consented before enrollment.

Results: The salivary flow rate and oral mucosal fraction of the study group after treatment were significantly improved, which was better than that of the control group(P < .05). The content of IL-10 in the study group after treatment increased significantly compared with that before treatment(P < .05). There was a significant difference between the oral flora of the study group before and after treatment (Unique OTU counts: 5390 vs 3906), and there was also a difference between the oral flora of the study group and control group after treatment (Unique OTU counts: 5671 vs 5439). After treatment, Erysipelotrichales (Phylum Firmicutes, LDA score = 2.80, P = .034), Leptotrichiaceae (Fusobacteria,LDA score = 3.38, P = .030) and Campylobacteraceae (Proteobacteria, LDA score = 3.35, P = .026) were significantly enriched in the study group. The use of Dendrobium officinale in nasopharyngeal carcinoma patients with radiotherapy-induced oral mucositis showed little difference in microbial diversity and abundance, but there were significant differences among oral bacteria genera.

Conclusions: Dendrobium officinale is effective in the treatment of radiotherapy-induced oral mucositis, which may be related to the improvement of salivary gland function and regulation of the oral microenvironment. Dendrobium officinale may reduce the symptoms of radiotherapy-induced oral mucositis by affecting the systemic cellular immune function. It may reduce the secretion of pro-inflammatory factors of the relevant flora by directly changing the oral flora and regulating the oral micro-ecology.