Pub Date : 2020-01-01DOI: 10.17706/ijbbb.2020.10.2.110-116
Kittiya Jiratanakittiwat, C. Satirapipathkul, D. Charnvanich
The aim of this study was to develop a process of extraction oxyresveratrol from Artocarpus lakoocha heartwood using a maceration method. The influences of types of solvent, a concentration of solvent, and time to extract on the yield of oxyresveratrol were studied. In addition, the antioxidant capacity of the extract was investigated. The quantitation of oxyresveratrol was measured by high-performance liquid chromatography (HPLC) analysis, and antioxidant activity was analyzed by 1,1’-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The results indicated that 70-percent ethanol solvent by volume for 6 hours could extract the optimum oxyresveratrol at 13.09 percent of the dry weight of Artocarpus lakoocha, and antioxidant efficiency had an IC50 of 47.70 μg/ml.
{"title":"The Influences of Extraction on the Quantity of Oxyresveratrol from Artocarpus lakoocha Roxb.","authors":"Kittiya Jiratanakittiwat, C. Satirapipathkul, D. Charnvanich","doi":"10.17706/ijbbb.2020.10.2.110-116","DOIUrl":"https://doi.org/10.17706/ijbbb.2020.10.2.110-116","url":null,"abstract":"The aim of this study was to develop a process of extraction oxyresveratrol from Artocarpus lakoocha heartwood using a maceration method. The influences of types of solvent, a concentration of solvent, and time to extract on the yield of oxyresveratrol were studied. In addition, the antioxidant capacity of the extract was investigated. The quantitation of oxyresveratrol was measured by high-performance liquid chromatography (HPLC) analysis, and antioxidant activity was analyzed by 1,1’-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The results indicated that 70-percent ethanol solvent by volume for 6 hours could extract the optimum oxyresveratrol at 13.09 percent of the dry weight of Artocarpus lakoocha, and antioxidant efficiency had an IC50 of 47.70 μg/ml.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"86 1","pages":"110-116"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84131581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.17706/ijbbb.2020.10.2.84-93
Shinji Kawakura, R. Shibasaki
Several physical characteristics of workers can be extracted from physical timeline data to understand acceleration and angular velocity. Although various approaches have been implemented globally for indoor and outdoor agricultural (agri-) working sites, there is room for improvement. In this study, we aim to adapt these approaches particularly for real agri-directors, leaders and managers to improve the quality of tasks and their security levels. Thus, we apply a deep learning-based method and qualitatively demonstrate the classification of physical timeline datasets. To create our dataset, our subjects were six experienced agri-manual workers and six completely inexperienced men. The targeted task was cultivating the semi-crunching position using a simple, Japanese-style hoe. We captured the subjects’ acceleration and angular velocity data from an integrated multi-sensor module mounted on a wood lilt 15 cm from the gripping position of the dominant hand. We used Python code and recent distributed libraries for computation. For data classification, we successively executed a Recurrent Neural Network (RNN), which we evaluated using wavelet analyses such as the Fast Fourier Transform (FFT). These methods of analyzing digital data could be of practical use for providing key suggestions to improve daily tasks.
{"title":"Deep Learning Based System to Extract Agricultural Workers’ Physical Timeline Data for Acceleration and Angular Velocity","authors":"Shinji Kawakura, R. Shibasaki","doi":"10.17706/ijbbb.2020.10.2.84-93","DOIUrl":"https://doi.org/10.17706/ijbbb.2020.10.2.84-93","url":null,"abstract":"Several physical characteristics of workers can be extracted from physical timeline data to understand acceleration and angular velocity. Although various approaches have been implemented globally for indoor and outdoor agricultural (agri-) working sites, there is room for improvement. In this study, we aim to adapt these approaches particularly for real agri-directors, leaders and managers to improve the quality of tasks and their security levels. Thus, we apply a deep learning-based method and qualitatively demonstrate the classification of physical timeline datasets. To create our dataset, our subjects were six experienced agri-manual workers and six completely inexperienced men. The targeted task was cultivating the semi-crunching position using a simple, Japanese-style hoe. We captured the subjects’ acceleration and angular velocity data from an integrated multi-sensor module mounted on a wood lilt 15 cm from the gripping position of the dominant hand. We used Python code and recent distributed libraries for computation. For data classification, we successively executed a Recurrent Neural Network (RNN), which we evaluated using wavelet analyses such as the Fast Fourier Transform (FFT). These methods of analyzing digital data could be of practical use for providing key suggestions to improve daily tasks.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"30 1","pages":"84-93"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82774303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.17706/ijbbb.2020.10.2.74-83
Justin S. Diamond
The desire for accurate protein prediction algorithms has been a hallmark of computational biology achievements. Still, better algorithms and methodologies can achieve even greater success with implication across a diverse range of biological and medicinal fields such as protein function inference. Accurate prediction methods rely heavily on sequence similarity, however structure is more evolutionary conserved, i.e. structure is an alternate characteristic for ancestral relationships between proteins. The premise of this work is that similar structural features will be clustered together, which may show a unique amino acid and secondary structure (SS) distribution, which can be, incorporated into HMMs for SS prediction and protein function inference algorithms. With structural-evolutionary relationship in mind, I propose a methodology for ‘structure’ based SS prediction methods using HMM and k-mean and fuzzy k -means fragmented protein clusters. When fragment distributions were incorporated into HMMs, the average accuracy increased by 1 percent but showed an increase in accuracy of up to 13 percent for particular sequences. The HMM results were not so promising, however the clustering of protein structure fragments by C-alphas bond angles shows to be a useful length-independent metric for inferring functional relationships between proteins.
{"title":"Analysis of Clustering Fragmented Protein Bond Angles","authors":"Justin S. Diamond","doi":"10.17706/ijbbb.2020.10.2.74-83","DOIUrl":"https://doi.org/10.17706/ijbbb.2020.10.2.74-83","url":null,"abstract":"The desire for accurate protein prediction algorithms has been a hallmark of computational biology achievements. Still, better algorithms and methodologies can achieve even greater success with implication across a diverse range of biological and medicinal fields such as protein function inference. Accurate prediction methods rely heavily on sequence similarity, however structure is more evolutionary conserved, i.e. structure is an alternate characteristic for ancestral relationships between proteins. The premise of this work is that similar structural features will be clustered together, which may show a unique amino acid and secondary structure (SS) distribution, which can be, incorporated into HMMs for SS prediction and protein function inference algorithms. With structural-evolutionary relationship in mind, I propose a methodology for ‘structure’ based SS prediction methods using HMM and k-mean and fuzzy k -means fragmented protein clusters. When fragment distributions were incorporated into HMMs, the average accuracy increased by 1 percent but showed an increase in accuracy of up to 13 percent for particular sequences. The HMM results were not so promising, however the clustering of protein structure fragments by C-alphas bond angles shows to be a useful length-independent metric for inferring functional relationships between proteins.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"10 1","pages":"74-83"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89564584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.33545/26646536.2020.v2.i1a.17
Meenakshi Sharma, Sb Sharma
Cancer becomes a global disease and there is no proper treatment of cancer. Natural Products plays important role in the drug discovery of cancer. The plants Tinospora cordifolia (common name guloy) have been investigated by various in vitro. However, detailed investigations on anticancer properties of the selected medicinal plants are unknown. In this direction, we have studied the detailed anticancer activities of Tinospora cordifolia in cancer cells (A349, PC3, Cervical Cancer) using a cell proliferation assay and the ethanolic extract of plant showed potent inhibition against cancer cells (A549, Hela and PC3) compare to aqueous extract of Tinospora cordifolia.
{"title":"Anticancer evaluation of Tinospora cordifolia extracts","authors":"Meenakshi Sharma, Sb Sharma","doi":"10.33545/26646536.2020.v2.i1a.17","DOIUrl":"https://doi.org/10.33545/26646536.2020.v2.i1a.17","url":null,"abstract":"Cancer becomes a global disease and there is no proper treatment of cancer. Natural Products plays important role in the drug discovery of cancer. The plants Tinospora cordifolia (common name guloy) have been investigated by various in vitro. However, detailed investigations on anticancer properties of the selected medicinal plants are unknown. In this direction, we have studied the detailed anticancer activities of Tinospora cordifolia in cancer cells (A349, PC3, Cervical Cancer) using a cell proliferation assay and the ethanolic extract of plant showed potent inhibition against cancer cells (A549, Hela and PC3) compare to aqueous extract of Tinospora cordifolia.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"148 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74769391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.17706/ijbbb.2020.10.2.66-73
Vipanee Chumsai-Na-Ayudhya, N. Thongwai
Miang (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam.) is an Assam tea which is mainly found in northern Thailand. Fermented Miang is made from young and semi-mature Miang leaves by the local wisdom. Briefly, Miang leaves are harvested and steamed for approximately 2 hours prior to cooling down and subsequently packed into a container with close lid. Fermentation process will take place and run for 1-3 months normally. Steaming step renders a high quantity of steamed Miang juices (SMJ) which will be discarded afterwards. Evaluation of this biological waste will bring about ways of making value-added products. The purpose of this study aims to investigate the effects of steaming time and ages of Miang leaves on biological activities of SMJ obtained. Fresh, young and semi-mature, Miang leaves were collected and steamed for 30 minutes, 1, 2 and 3 hours. Meanwhile, young and old Miang leaves were steamed for 2 hours as compared with the conventional method. All SMJ were further evaporated and lyophilized to obtain SMJ crude extracts (CE). The 3-hour SMJ-CE had the highest antioxidant activity and total phenolic content (p<0.05), 548.4±13 μg GAE/ g extract and 460.24±11.8 μg GAE/g extract, respectively. Comparing between SMJ-CE of young and old Miang leaves, the antioxidant activity and total phenolic content of the young leaves SMJ-CE was significantly higher than the old leaves SMJ-CE (p<0.05). Moreover, all SMJ-CE had antibacterial activities to inhibit growth of some gastrointestinal pathogenic bacteria including Bacillus cereus, Escherichia coli, Escherichia coli O157:H7, Salmonella Typhi, Shigella dysenteriae, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA) and Vibrio cholerae with the young leaves SMJ-CE showed the highest activities. The information obtained will be further used as a guideline in food and cosmetic application using SMJ-CE as a raw material.
{"title":"Biological Activities of Steamed Miang (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam) Juice Extracts","authors":"Vipanee Chumsai-Na-Ayudhya, N. Thongwai","doi":"10.17706/ijbbb.2020.10.2.66-73","DOIUrl":"https://doi.org/10.17706/ijbbb.2020.10.2.66-73","url":null,"abstract":"Miang (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam.) is an Assam tea which is mainly found in northern Thailand. Fermented Miang is made from young and semi-mature Miang leaves by the local wisdom. Briefly, Miang leaves are harvested and steamed for approximately 2 hours prior to cooling down and subsequently packed into a container with close lid. Fermentation process will take place and run for 1-3 months normally. Steaming step renders a high quantity of steamed Miang juices (SMJ) which will be discarded afterwards. Evaluation of this biological waste will bring about ways of making value-added products. The purpose of this study aims to investigate the effects of steaming time and ages of Miang leaves on biological activities of SMJ obtained. Fresh, young and semi-mature, Miang leaves were collected and steamed for 30 minutes, 1, 2 and 3 hours. Meanwhile, young and old Miang leaves were steamed for 2 hours as compared with the conventional method. All SMJ were further evaporated and lyophilized to obtain SMJ crude extracts (CE). The 3-hour SMJ-CE had the highest antioxidant activity and total phenolic content (p<0.05), 548.4±13 μg GAE/ g extract and 460.24±11.8 μg GAE/g extract, respectively. Comparing between SMJ-CE of young and old Miang leaves, the antioxidant activity and total phenolic content of the young leaves SMJ-CE was significantly higher than the old leaves SMJ-CE (p<0.05). Moreover, all SMJ-CE had antibacterial activities to inhibit growth of some gastrointestinal pathogenic bacteria including Bacillus cereus, Escherichia coli, Escherichia coli O157:H7, Salmonella Typhi, Shigella dysenteriae, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA) and Vibrio cholerae with the young leaves SMJ-CE showed the highest activities. The information obtained will be further used as a guideline in food and cosmetic application using SMJ-CE as a raw material.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"1 1","pages":"66-73"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88969214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.17706/ijbbb.2020.10.2.117-126
Enwkwe Chukwudi Benedict, T. O. Chime, E. Osoka
{"title":"Optimization and Effect of Leaves Extracts on Corrosion of Mild Steal in Acidic Medium","authors":"Enwkwe Chukwudi Benedict, T. O. Chime, E. Osoka","doi":"10.17706/ijbbb.2020.10.2.117-126","DOIUrl":"https://doi.org/10.17706/ijbbb.2020.10.2.117-126","url":null,"abstract":"","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"43 1","pages":"117-126"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82149738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.33545/26646536.2019.v1.i2a.5
R. T, Guinto Edgardo R, Jorduela Eric L, Olaco John Paul Q, Santos Christian V, Madrid Francis Gerald N
{"title":"Phenotypic characterization and presumptive identification of bacterial isolates from moribund Nile tilapia (Oreochromis niloticus L.)","authors":"R. T, Guinto Edgardo R, Jorduela Eric L, Olaco John Paul Q, Santos Christian V, Madrid Francis Gerald N","doi":"10.33545/26646536.2019.v1.i2a.5","DOIUrl":"https://doi.org/10.33545/26646536.2019.v1.i2a.5","url":null,"abstract":"","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90161915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.33545/26646536.2019.v1.i2a.9
E. Un, Enaregha Eb
{"title":"Mycoflora associated with brachystegia eurycoma (Achi) seeds and their effects on the biochemical properties of the seed","authors":"E. Un, Enaregha Eb","doi":"10.33545/26646536.2019.v1.i2a.9","DOIUrl":"https://doi.org/10.33545/26646536.2019.v1.i2a.9","url":null,"abstract":"","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88655143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.33545/26646536.2019.v1.i2a.7
A. Hossain, M. M. Uddin
The purpose of the study was to evaluate the callus cell weight, antioxidant, carbohydrate, pigment and nutritional properties from broccoli explants as nutritious vegetable. The results showed that the higher callus weight was found in the cultured leaf cutting than root and shoot tips cutting in the concentration of IBA 1.0 + 2.0 mg/l BAP, IBA 2.0 + 2.0 mg/l BAP, and IBA 3.0 + 2.0 mg/l BAP combination. The highest callus weight was found in the cultured leaf cutting than root and shoot tips cutting at the concentration of IBA 2.0 + 2.0 mg/l BAP. Carbohydrate (as inverted sugar and glucose), chlorophyll and nutrient content (K+, NO3-, Ca++ and Na+), total phenol, antioxidant (DPPH), flavonoid and total antioxidant were found in the concentration of IBA 2.0 + 2.0 mg/l BAP combination from broccoli leaf cutting. The results seemed that it was better for nutrition and growth to use the combination of the IBA and BAP in the concentration of 1.0-3.0 mg/l to regenerate root, shoot, leaf and callus cell proliferation and nutrition of broccoli from root tip, shoot tip and leaf cutting.
{"title":"Callus cell weight, antioxidant, carbohydrate, pigment and nutritional properties from broccoli explants in vitro: A nutritional vegetable","authors":"A. Hossain, M. M. Uddin","doi":"10.33545/26646536.2019.v1.i2a.7","DOIUrl":"https://doi.org/10.33545/26646536.2019.v1.i2a.7","url":null,"abstract":"The purpose of the study was to evaluate the callus cell weight, antioxidant, carbohydrate, pigment and nutritional properties from broccoli explants as nutritious vegetable. The results showed that the higher callus weight was found in the cultured leaf cutting than root and shoot tips cutting in the concentration of IBA 1.0 + 2.0 mg/l BAP, IBA 2.0 + 2.0 mg/l BAP, and IBA 3.0 + 2.0 mg/l BAP combination. The highest callus weight was found in the cultured leaf cutting than root and shoot tips cutting at the concentration of IBA 2.0 + 2.0 mg/l BAP. Carbohydrate (as inverted sugar and glucose), chlorophyll and nutrient content (K+, NO3-, Ca++ and Na+), total phenol, antioxidant (DPPH), flavonoid and total antioxidant were found in the concentration of IBA 2.0 + 2.0 mg/l BAP combination from broccoli leaf cutting. The results seemed that it was better for nutrition and growth to use the combination of the IBA and BAP in the concentration of 1.0-3.0 mg/l to regenerate root, shoot, leaf and callus cell proliferation and nutrition of broccoli from root tip, shoot tip and leaf cutting.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80013181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.33545/26646536.2019.v1.i2a.8
A. Hossain, M. M. Uddin
{"title":"Plant hormones as cell signaling component, its mechanism and regulated gene expression","authors":"A. Hossain, M. M. Uddin","doi":"10.33545/26646536.2019.v1.i2a.8","DOIUrl":"https://doi.org/10.33545/26646536.2019.v1.i2a.8","url":null,"abstract":"","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74356507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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