Pub Date : 2018-12-04DOI: 10.24843/IJBB.2018.V06.I01.P06
Hee-Wan Kang
Twenty primers of 20 mer referred to universal rice primer (URP) were developed from a repetitive sequence of rice genome. URP-PCR protocol employed stringent PCR with high annealing temperature throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, each single URP primer produced characteristic fingerprints from diverse genomes of bacterial species. The universal application of URP-PCR was demonstrated by applying it to 24 strains from Pectobacterium carotovoum subsp. carotovorum, 41 Agrobacterium vitis strains, 3 Xanthomonas spp. 5 Pseudomonas spp, Rhizobium sp. plant pathogenic bacteria, human and animal pathogenic bacterial strains including 6 Escherichia coli, 4 Salmonella spp., 7 Mycobacterium spp and 3 Blucella abortus strains. In addition, thermophilic bacteria were randomly isolated form high temperature compost and their URP-PCR polymorphisms were characterized with genetic relatedness. PCR approach using URP primers will be useful for studying DNA diversity of diverse prokaryotic genomes, especially at inter- and intra species levels.
{"title":"PCR FINGERPRINTING OF DIVERSE GENOMES FROM BACTERIAL STRAINS USING UNIVERSAL RICE PRIMER (URP)","authors":"Hee-Wan Kang","doi":"10.24843/IJBB.2018.V06.I01.P06","DOIUrl":"https://doi.org/10.24843/IJBB.2018.V06.I01.P06","url":null,"abstract":"Twenty primers of 20 mer referred to universal rice primer (URP) were developed from a repetitive sequence of rice genome. URP-PCR protocol employed stringent PCR with high annealing temperature throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, each single URP primer produced characteristic fingerprints from diverse genomes of bacterial species. The universal application of URP-PCR was demonstrated by applying it to 24 strains from Pectobacterium carotovoum subsp. carotovorum, 41 Agrobacterium vitis strains, 3 Xanthomonas spp. 5 Pseudomonas spp, Rhizobium sp. plant pathogenic bacteria, human and animal pathogenic bacterial strains including 6 Escherichia coli, 4 Salmonella spp., 7 Mycobacterium spp and 3 Blucella abortus strains. In addition, thermophilic bacteria were randomly isolated form high temperature compost and their URP-PCR polymorphisms were characterized with genetic relatedness. PCR approach using URP primers will be useful for studying DNA diversity of diverse prokaryotic genomes, especially at inter- and intra species levels.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78830968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-04DOI: 10.24843/IJBB.2018.V06.I01.P03
I. Sudiarta, Dwi Martiningsia, I. Wijaya
Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.
{"title":"MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE","authors":"I. Sudiarta, Dwi Martiningsia, I. Wijaya","doi":"10.24843/IJBB.2018.V06.I01.P03","DOIUrl":"https://doi.org/10.24843/IJBB.2018.V06.I01.P03","url":null,"abstract":"Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76042036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-04DOI: 10.24843/ijbb.2018.v06.i01.p04
Nyoman Nila Arieswari, I. Astarini, Ni Putu Adriani Astiti, Jeremy Pramana
One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.
{"title":"IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION","authors":"Nyoman Nila Arieswari, I. Astarini, Ni Putu Adriani Astiti, Jeremy Pramana","doi":"10.24843/ijbb.2018.v06.i01.p04","DOIUrl":"https://doi.org/10.24843/ijbb.2018.v06.i01.p04","url":null,"abstract":"One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89799319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-04DOI: 10.24843/ijbb.2018.v06.i01.p02
N. Triwahyuningsih, T. B. Kusmiyarti
Kudzu plantation (Pueraria phaseoloides) as legume cover crop is one of alternatives in coastal sandy land conservation. The crops are known to associate with a root nodule-forming bacteria (Rhizobium sp.) which give some benefits to nutrient cycling i.e. : atmospheric N2 fixing and play role as soil conditioner; soil Nitrogen enrichment; nutrient cycling; and increasing other nutrients availability. A research to study the isolation dan bacterial inoculum multiplication from wild kudzu root nodules, compatible isolates screening and selected isolates multiplication, and examining the form, amounts and most proper inoculum application method was conducted in Greenhouse and Laboratory of Microbiology in Yogyakarta province.The research were held in four phases : (1) isolation, purification and characterization of isolates; (2) reinoculation dan compatibility testing of isolates to kudzu seeds; (3) inoculum multiplication; and (4) examination of the form, amounts and most proper inoculum application method. Physical and biochemical properties of the isolates were observed during the isolation phase. Infection and nodulation activity were observed during the reinoculation phase. Indirectly counting of the microbial numbers to obtain the cell numbers was conducted during the inoculum multiplication. While infection and nodulation activity and plant growth were observed during the inoculum testing phase.Isolates purification on Yeast Mannitol Agar + congo-red media gave 5 different isolates named RP-Etp1, RP-Etp2, RP-Etp3, RP-Etp4, RP-Etp5. The RP-Etp4 isolate had the highest compatibility to the kudzu seeds (number of effective nodules >100 per plant), followed by RP-Etp5 (medium compatibility, number of effective nodules 50–100 per plant), RP-Etp1 and RP-Etp3 (low compatibility, number of effective nodules < 10). Isolate RP-Etp2 was incompatible to the kudzu.Optimum cell numbers was reached in 48 hours incubation time. Application of broth/liquid inoculum of Rhizobium sp. has advantages over solid inoculum (in peat) as it gives the highest number of nodules, and the optimum dosage was 2 – 4 ml per plant. The highest infection-nodulation activity and plant growth were reached in 4 ml inoculum per plant (direct application) or 2 ml inoculum per plant (weekly applied in two weeks).
{"title":"ISOLATION, CHARACTERIZATION AND INOCULUM FORMULATION OF NODULE FORMING BACTERIA OF KUDZU (Pueraria phaseoloides (Roxb.)Benth.) FOR COASTAL SANDY LAND CONSERVATION","authors":"N. Triwahyuningsih, T. B. Kusmiyarti","doi":"10.24843/ijbb.2018.v06.i01.p02","DOIUrl":"https://doi.org/10.24843/ijbb.2018.v06.i01.p02","url":null,"abstract":"Kudzu plantation (Pueraria phaseoloides) as legume cover crop is one of alternatives in coastal sandy land conservation. The crops are known to associate with a root nodule-forming bacteria (Rhizobium sp.) which give some benefits to nutrient cycling i.e. : atmospheric N2 fixing and play role as soil conditioner; soil Nitrogen enrichment; nutrient cycling; and increasing other nutrients availability. A research to study the isolation dan bacterial inoculum multiplication from wild kudzu root nodules, compatible isolates screening and selected isolates multiplication, and examining the form, amounts and most proper inoculum application method was conducted in Greenhouse and Laboratory of Microbiology in Yogyakarta province.The research were held in four phases : (1) isolation, purification and characterization of isolates; (2) reinoculation dan compatibility testing of isolates to kudzu seeds; (3) inoculum multiplication; and (4) examination of the form, amounts and most proper inoculum application method. Physical and biochemical properties of the isolates were observed during the isolation phase. Infection and nodulation activity were observed during the reinoculation phase. Indirectly counting of the microbial numbers to obtain the cell numbers was conducted during the inoculum multiplication. While infection and nodulation activity and plant growth were observed during the inoculum testing phase.Isolates purification on Yeast Mannitol Agar + congo-red media gave 5 different isolates named RP-Etp1, RP-Etp2, RP-Etp3, RP-Etp4, RP-Etp5. The RP-Etp4 isolate had the highest compatibility to the kudzu seeds (number of effective nodules >100 per plant), followed by RP-Etp5 (medium compatibility, number of effective nodules 50–100 per plant), RP-Etp1 and RP-Etp3 (low compatibility, number of effective nodules < 10). Isolate RP-Etp2 was incompatible to the kudzu.Optimum cell numbers was reached in 48 hours incubation time. Application of broth/liquid inoculum of Rhizobium sp. has advantages over solid inoculum (in peat) as it gives the highest number of nodules, and the optimum dosage was 2 – 4 ml per plant. The highest infection-nodulation activity and plant growth were reached in 4 ml inoculum per plant (direct application) or 2 ml inoculum per plant (weekly applied in two weeks).","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76288220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-04DOI: 10.24843/ijbb.2018.v06.i01.p05
Muhammad Ibadullah, I. Astarini, E. Kriswiyanti
Potato is one of the main carbohydrate sources around the world, including Indonesia. Potato production in Bali generally does not use good quality of potato seed, causing disease infection and reduce productivity. An alternative effort to produce high quality potato is by induce mutation of tuber using gamma ray irradiation. This study aims to find out percentage of survival after irradiation of ‘Granola’ potato shoots and determine the post-irradiation potato growth and productivity. This research was conducted at Laboratory of Central Application of Isotope and Irradiation (PAIR), Pasar Jumat, Batan, Jakarta and UPT BBITPH Bedugul, Bali. Planting materials were early generation (G0) potato seed tubers. This study employ completely randomized factorial design with one factor, i.e. irradiation doses of 0, 20 gy and 40 Gy. Variable observed included percentage of shoots survive, and variations in production. Results showed that 20 Gy was the best dose to increase tuber production.
{"title":"INCREASE VARIATION ON POTATO ‘GRANOLA’ USING GAMMA RAY IRRADIATION","authors":"Muhammad Ibadullah, I. Astarini, E. Kriswiyanti","doi":"10.24843/ijbb.2018.v06.i01.p05","DOIUrl":"https://doi.org/10.24843/ijbb.2018.v06.i01.p05","url":null,"abstract":"Potato is one of the main carbohydrate sources around the world, including Indonesia. Potato production in Bali generally does not use good quality of potato seed, causing disease infection and reduce productivity. An alternative effort to produce high quality potato is by induce mutation of tuber using gamma ray irradiation. This study aims to find out percentage of survival after irradiation of ‘Granola’ potato shoots and determine the post-irradiation potato growth and productivity. This research was conducted at Laboratory of Central Application of Isotope and Irradiation (PAIR), Pasar Jumat, Batan, Jakarta and UPT BBITPH Bedugul, Bali. Planting materials were early generation (G0) potato seed tubers. This study employ completely randomized factorial design with one factor, i.e. irradiation doses of 0, 20 gy and 40 Gy. Variable observed included percentage of shoots survive, and variations in production. Results showed that 20 Gy was the best dose to increase tuber production.","PeriodicalId":13910,"journal":{"name":"International Journal of Biosciences and Biotechnology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85343912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：