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RETRACTION NOTICE: Tripterine up-regulates miR-223 to alleviate lipopolysaccharide-induced damage in murine chondrogenic ATDC5 cells. 撤回注意:雷公藤红素上调miR-223以减轻脂多糖诱导的小鼠软骨性ATDC5细胞损伤。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211040396
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引用次数: 0
The 30th birthday of chronic ulcerative stomatitis: A systematic review. 慢性溃疡性口炎30岁生日:一项系统综述。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211052437
Astrid Herzum, Martina Burlando, Emanuele Cozzani, Aurora Parodi

Objectives: Chronic ulcerative stomatitis (CUS) is a chronic, ulcerative condition of the oral cavity, clinically and histologically similar to oral lichen planus (OLP), first described as a new disease entity in 1990 by Parodi et al. In this review, 30 years after our first description of CUS, we aimed to systematically review the literature of CUS cases reported ever since.

Methods: We present a systematic review of CUS literature cases, performed in compliance with the PRISMA statement.

Results: Of 125 retrieved articles, 20 satisfied inclusion criteria. These described 76 CUS cases, all presenting orally evident disease: erosions (55%), white lesions (49%), erythema (49%), ulcerations (34%) were the most frequent signs; 54% experienced discomfort/pain. Topographically, buccal mucosa (68%) and gingiva (54%) were the most affected locations, followed by tongue (42%), hard palate (27%), labial mucosa (22%), and widespread involvement (15%). Great diagnostic delay (6.3 years) was evidenced highlighting CUS is an entity too often misdiagnosed. Histopathology found lichenoid features (46%) and non-specific inflammation (54%). Extra-oral involvement was reported in 21%, especially as LP (69%). Of DIF, 97% were positive; 3% negative, compensated by positive IIF, permitting diagnosis. Of patients on steroids, only 12% reported therapeutic success; most steroid-non-responsive patients passed to antimalarials, with 91.66% success when used alone, 100% success in combination therapy.

Conclusion: Dermatologists should suspect CUS in chronic steroid-unresponsive erosive/ulcerative stomatitis. In these cases, to diagnose CUS, the presence of stratified epithelium-specific antinuclear antibodies (SES-ANA) should be investigated through immunofluorescence. Once diagnosed, CUS can be treated with antimalarials, which are an effective treatment contrarily to corticosteroids.

目的:慢性溃疡性口炎(CUS)是一种口腔慢性溃疡性疾病,临床和组织学上与口腔扁平苔藓(OLP)相似,Parodi等人于1990年首次将其描述为一种新的疾病实体。在这篇综述中,在我们首次描述CUS 30年后,我们旨在系统地回顾自那时以来报道的CUS病例的文献。方法:我们根据PRISMA声明对CUS文献病例进行系统回顾。结果:125篇检索文献中,20篇符合纳入标准。本文描述了76例CUS病例,均表现为口腔明显疾病:最常见的症状是糜烂(55%)、白色病变(49%)、红斑(49%)、溃疡(34%);54%的人感到不适/疼痛。从地形上看,口腔黏膜(68%)和牙龈(54%)是最受影响的部位,其次是舌头(42%)、硬腭(27%)、唇黏膜(22%)和广泛受累(15%)。诊断延迟大(6.3年),突出了CUS是一个经常误诊的实体。组织病理学发现地衣样特征(46%)和非特异性炎症(54%)。口腔外受累21%,尤其是LP(69%)。97%的DIF阳性;3%阴性,IIF阳性补偿,允许诊断。服用类固醇的患者中,只有12%报告治疗成功;大多数类固醇无反应的患者转而使用抗疟药物,单独使用时成功率为91.66%,联合治疗时成功率为100%。结论:皮肤科医生应怀疑慢性类固醇无反应性糜烂性/溃疡性口炎为CUS。在这些病例中,为了诊断CUS,应通过免疫荧光检测分层上皮特异性抗核抗体(SES-ANA)的存在。一旦确诊,可使用抗疟药物治疗,这是一种与皮质类固醇相反的有效治疗方法。
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引用次数: 1
The correlation between lipoprotein associated phospholipase A2 and central overweight status. 脂蛋白相关磷脂酶 A2 与中心超重状态之间的相关性。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211048562
Yi-Hsuan Chen, Wen-Cheng Li, Yi-Chuan Chen, Wei-Chung Yeh, Wei Yu, Hsiung Ying Hung, Xiong-Xue Jie, Jau-Yuan Chen

Objective: Being overweight is associated with an increased risk of diabetes mellitus, hypertension, and cardiovascular disease. Lipoprotein-associated phospholipase A2 (Lp-PLA2) can independently predict the risk of cardiovascular disease. This study is aimed to investigate whether Lp-PLA2 was associated with an overweight status.

Methods: This was a cross-sectional study that enrolled 3760 Chinese adults (age, 18-50 years) who underwent medical examination department of Xiamen Chang-Gung Hospital (XCGH) from 2018 to 2020. To explore the distribution of overweight classifications in the Chinese population, we evaluated the correlation of the overweight status with Lp-PLA2, after correcting for possible influencing factors.

Results: The Lp-PLA2 level was greater in male than in female subjects (p < 0.001). Subjects with a central overweight status had a greater Lp-PLA2 level than those with normal weight and a peripheral overweight status, in both male and female cohorts. The Lp-PLA2 level was significantly greater in those with additional comorbidities (namely diabetes mellitus (DM), hypertension (HTN), overweight, and metabolic syndrome (MetS)). The age-adjusted and LDL-adjusted Lp-PLA2 level also was significantly higher in the DM (+) and HTN (-) subgroups than in the DM (-), HTN (-), DM (-), and HTN (+) subgroups.

Conclusion: Lp-PLA2 is associated with sex, central overweight status, diabetes, hypertension, and MetS in adults aged < 50 years and the age-adjusted and LDL-adjusted Lp-PLA2 was significantly higher in the DM (+) and HTN (-) subgroups than in the DM (-) and HTN (-) and DM (-) and HTN (+) subgroups.

目的:超重会增加罹患糖尿病、高血压和心血管疾病的风险。脂蛋白相关磷脂酶 A2(Lp-PLA2)可独立预测心血管疾病的风险。本研究旨在探讨 Lp-PLA2 是否与超重状态有关:这是一项横断面研究,共纳入2018年至2020年期间在厦门长庚医院(XCGH)体检科接受体检的3760名中国成年人(年龄,18-50岁)。为了探索中国人群中超重分类的分布情况,我们在校正了可能的影响因素后,评估了超重状态与Lp-PLA2的相关性:结果:男性的脂蛋白磷酸酶水平高于女性(P < 0.001)。与体重正常和外周超重的受试者相比,在男性和女性群体中,中心超重受试者的脂蛋白-PLA2水平更高。伴有其他合并症(即糖尿病(DM)、高血压(HTN)、超重和代谢综合征(MetS))者的 Lp-PLA2 水平明显更高。经年龄调整和低密度脂蛋白调整后,DM(+)和高血压(-)亚组的脂蛋白-PLA2水平也明显高于DM(-)、高血压(-)、DM(-)和高血压(+)亚组:Lp-PLA2与50岁以下成年人的性别、中心超重状态、糖尿病、高血压和MetS有关,经年龄调整和低密度脂蛋白调整的Lp-PLA2在DM(+)和HTN(-)亚组中显著高于DM(-)和HTN(-)亚组以及DM(-)和HTN(+)亚组。
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引用次数: 0
Blockade of LINC01605-enriched exosome generation in M2 macrophages impairs M2 macrophage-induced proliferation, migration, and invasion of human dermal fibroblasts. 阻断M2巨噬细胞中富集linc01605的外泌体生成可损害M2巨噬细胞诱导的人真皮成纤维细胞的增殖、迁移和侵袭。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211016724
Zhensen Zhu, Bo Chen, Liang Peng, Songying Gao, Jingdong Guo, Xiongxiang Zhu

Activated M2 macrophages are involved in hypertrophic scar (HS) formation via manipulating the differentiation of fibroblasts to myofibroblasts having the proliferative capacity and biological function. However, the function of exosomes derived from M2 macrophages in HS formation is unclear. Thus, this study aims to investigate the role of exosomes derived by M2 in the formation of HS. To understand the effect of exosomes derived from M2 macrophages on formation of HS, M2 macrophages were co-cultured with human dermal fibroblast (HDF) cells. Cell Counting Kit-8 assay was performed to evaluate HDF proliferation. To evaluate the migration and invasion of HDFs, wound-healing and transwell invasion assays were performed, respectively. To investigate the interaction between LINC01605 and miR-493-3p, a dual-luciferase reporter gene assay was adopted; consequently, an interaction between miR-493-3p and AKT1 was detected. Our results demonstrated that exosomes derived from M2 macrophages promoted the proliferation, migration, and invasion of HDFs. Additionally, we found that long noncoding RNA LINC01605, enriched in exosomes derived from M2 macrophages, promoted fibrosis of HDFs and that GW4869, an inhibitor of exosomes, could revert this effect. Mechanistically, LINC01605 promoted fibrosis of HDFs by directly inhibiting the secretion of miR-493-3p, and miR-493-3p down-regulated the expression of AKT1. Exosomes derived from M2 macrophages promote the proliferation and migration of HDFs by transmitting LINC01605, which may activate the AKT signaling pathway by sponging miR-493-3p. Our results provide a novel approach and basis for further investigation of the function of M2 macrophages in HS formation.

活化的M2巨噬细胞通过调控成纤维细胞向具有增殖能力和生物学功能的肌成纤维细胞的分化,参与肥厚性瘢痕(HS)的形成。然而,来自M2巨噬细胞的外泌体在HS形成中的功能尚不清楚。因此,本研究旨在探讨M2衍生的外泌体在HS形成中的作用。为了了解M2巨噬细胞衍生的外泌体对HS形成的影响,我们将M2巨噬细胞与人真皮成纤维细胞(HDF)共培养。细胞计数试剂盒-8检测HDF增殖情况。为了评估HDFs的迁移和侵袭,分别进行了伤口愈合和跨井侵袭试验。为了研究LINC01605与miR-493-3p之间的相互作用,采用双荧光素酶报告基因检测;因此,miR-493-3p和AKT1之间的相互作用被检测到。我们的研究结果表明,来自M2巨噬细胞的外泌体促进了HDFs的增殖、迁移和侵袭。此外,我们发现长链非编码RNA LINC01605富集于来自M2巨噬细胞的外泌体中,促进HDFs的纤维化,而外泌体抑制剂GW4869可以恢复这一作用。机制上,LINC01605通过直接抑制miR-493-3p的分泌促进HDFs纤维化,miR-493-3p下调AKT1的表达。来源于M2巨噬细胞的外泌体通过传递LINC01605促进HDFs的增殖和迁移,LINC01605可能通过海绵化miR-493-3p激活AKT信号通路。本研究结果为进一步研究M2巨噬细胞在HS形成中的作用提供了新的途径和基础。
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引用次数: 10
Follicle stimulating hormone and estradiol alter immune response in osteoarthritic mice in an opposite manner. 促卵泡激素和雌二醇以相反的方式改变骨关节炎小鼠的免疫反应。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211016198
Lyudmila Belenska-Todorova, Ralitsa Zhivkova, Maya Markova, Nina Ivanovska

Although a number of studies have shown that the occurrence and progression of osteoarthritis (OA) is related to endocrine system dysfunction, there is limited evidence about what roles sex hormones play. The aim of the present study was to examine the capacity of 17β-estradiol (ED) and follicle stimulating hormone (FSH) to alter the differentiation of bone marrow (BM) cells in arthritic mice. The experiments were conducted in collagenase-induced osteoarthritis in mice. Cartilage degradation was observed by safranin and toluidine blue staining. Flow cytometry was used to define different BM and synovial cell populations. The influence of FSH and ED on osteoclastogenesis was studied in BM cultures and on the osteoblastogenesis in primary calvarial cultures. The levels of IL-8, TNF-α, FSH, and osteocalcin were estimated by ELISA. FSH increased cartilage degradation and serum osteocalcin levels, while ED abolished it and lowered serum osteocalcin. FSH elevated the percentage of monocytoid CD14+/RANK+ and B cell CD19+/RANK+ cells in contrast to ED which inhibited the accumulation of these osteogenic populations. Also, ED changed the percentage of CD105+/F4/80+ and CD11c+ cells in the synovium. FSH augmented and ED suppressed macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast (OC) formation, and this correlated with a respective increase and decrease of IL-8 secretion. FSH did not influence osteoblast (OB) formation while ED enhanced this process in association with changes of TNF-α, IL-8, and osteocalcin production. ED reduced osteoclast generation in bone. The key outcome of the current study is that both hormones influenced BM cell differentiation, with FSH favoring osteoclast formation and ED favoring osteoblast accumulation.

尽管许多研究表明,骨关节炎(OA)的发生和发展与内分泌系统功能障碍有关,但关于性激素在其中发挥何种作用的证据却很有限。本研究旨在探讨 17β-雌二醇(ED)和促卵泡激素(FSH)改变关节炎小鼠骨髓(BM)细胞分化的能力。实验是在胶原酶诱导的小鼠骨关节炎中进行的。通过黄绿素和甲苯胺蓝染色观察软骨降解。流式细胞术用于确定不同的基质细胞和滑膜细胞群。研究了FSH和ED对BM培养物中破骨细胞生成的影响,以及对原代钙质培养物中成骨细胞生成的影响。通过酶联免疫吸附测定了IL-8、TNF-α、FSH和骨钙素的水平。FSH增加了软骨降解和血清骨钙素水平,而ED抑制了软骨降解并降低了血清骨钙素水平。FSH 增加了单核细胞 CD14+/RANK+ 和 B 细胞 CD19+/RANK+ 的比例,而 ED 则抑制了这些成骨细胞群的聚集。此外,ED还改变了滑膜中CD105+/F4/80+和CD11c+细胞的比例。FSH增加了巨噬细胞集落刺激因子(M-CSF)+核因子κB配体受体激活剂(RANKL)诱导的破骨细胞(OC)的形成,而ED则抑制了这种形成,这与IL-8分泌的增加和减少有关。FSH 不影响成骨细胞(OB)的形成,而 ED 则增强了这一过程,并与 TNF-α、IL-8 和骨钙素分泌的变化相关联。ED 可减少骨中破骨细胞的生成。本研究的主要结果是,这两种激素都会影响基础母细胞的分化,其中 FSH 有利于破骨细胞的形成,而 ED 有利于成骨细胞的积累。
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引用次数: 0
Melatonin ameliorates hepatic steatosis by inhibiting NLRP3 inflammasome in db/db mice. 褪黑素通过抑制NLRP3炎性体改善db/db小鼠肝脂肪变性。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211036819
Yongxiang Yu, Dongru Chen, Yuhua Zhao, Jianjun Zhu, Xiaohui Dong

Introduction: Type 2 diabetes mellitus (T2DM) is commonly accompanied by obesity and non-alcoholic fatty liver disease (NAFLD), yet the mechanism underlying diabetes-related NAFLD is not fully understood. It has been reported that melatonin can regulate glucose and lipid metabolism. This study aims to investigate the actions and mechanisms of melatonin toward the development of diabetes-related NAFLD.

Methods: Melatonin (bid, 30 mg/kg/day, i.p.) was administrated to db/db mice for 8 weeks, while saline was administrated to db/m mice. The metabolic parameters of mice were measured using an automatic biochemistry analyzer. The oxidative stress indexes and mitochondrial membrane potential (MMP) were determined with kits. Pathological assessment in liver tissues was used to analyze the effects of melatonin on hepatic steatosis. The levels of IL-1β and IL-18 were detected with ELISA kits. The mRNA levels of NLRP3 inflammasome were detected using quantitative real-time PCR assay, and protein expressions were estimated using Western blotting assay. Immunofluorescence staining was used to evaluate the caspase-1 expression in the liver.

Results: Melatonin treatment significantly reduced blood glucose, serum insulin, body weight, related liver weight, serum lipids, and hepatic enzymes in db/db mice. Melatonin markedly corrected the NAFLD phenotypes, including lipid accumulation, steatohepatitis, fibrosis, and oxidative stress levels. Melatonin significantly improved the MMP level and decreased the serum IL-1β and IL-18 concentrations. The mRNA levels of the NLRP3 inflammasome could also be remarkably reversed by melatonin in the liver tissues. The activation of the NLRP3 inflammasome was also suppressed, evidenced by the downregulated proteins of NLRP3, caspase-1, IL-1β, and IL-18. The enhanced fluorescence intensity of caspase-1 in the liver tissues was also obviously weakened by the melatonin treatment.

Conclusion: Our study concluded that melatonin could safeguard against NAFLD by improving hepatic steatosis in db/db mice, and this action could be associated with the regulation of the NLRP3 inflammasome activation.

2型糖尿病(T2DM)通常伴有肥胖和非酒精性脂肪性肝病(NAFLD),但糖尿病相关NAFLD的发病机制尚不完全清楚。据报道,褪黑激素可以调节葡萄糖和脂质代谢。本研究旨在探讨褪黑素在糖尿病相关NAFLD发生中的作用及机制。方法:db/db小鼠ig褪黑素(bid, 30 mg/kg/day, i.p.) 8周,db/m小鼠ig生理盐水。采用自动生化分析仪测定小鼠的代谢参数。用试剂盒测定氧化应激指标和线粒体膜电位(MMP)。采用肝组织病理学评价分析褪黑素对肝脂肪变性的影响。采用ELISA试剂盒检测IL-1β、IL-18水平。采用实时荧光定量PCR法检测NLRP3炎性小体mRNA水平,Western blotting法检测蛋白表达。免疫荧光染色法检测caspase-1在肝脏中的表达。结果:褪黑素治疗显著降低db/db小鼠的血糖、血清胰岛素、体重、相关肝重、血脂和肝酶。褪黑素显著纠正NAFLD表型,包括脂质积累、脂肪性肝炎、纤维化和氧化应激水平。褪黑素显著提高MMP水平,降低血清IL-1β和IL-18浓度。肝脏组织中NLRP3炎症小体的mRNA水平也可以被褪黑素显著逆转。NLRP3炎症小体的激活也被抑制,NLRP3、caspase-1、IL-1β和IL-18的蛋白下调证明了这一点。褪黑素也明显减弱了肝组织caspase-1荧光强度的增强。结论:我们的研究表明,褪黑素可以通过改善db/db小鼠的肝脏脂肪变性来预防NAFLD,这种作用可能与调节NLRP3炎性小体的激活有关。
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引用次数: 15
A retrospective study on the prevalence of anti-phospholipid antibodies, thrombotic events and cutaneous signs of vasculopathy in 173 hospitalized COVID-19 patients. 173例住院COVID-19患者抗磷脂抗体、血栓形成事件及血管病变皮肤体征的回顾性研究
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211042115
Giulia Gasparini, Paola Canepa, Simonetta Verdiani, Luca Carmisciano, Emanuele Cozzani, Denise De Grazia, Orsi Andrea, Giancarlo Icardi, Aurora Parodi

Background: Hypercoagulability is a risk factor of thromboembolic events in COVID-19. Anti-phospholipid (aPL) antibodies have been hypothesized to be involved. Typical COVID-19 dermatological manifestations of livedo reticularis and digital ischemia may resemble cutaneous manifestations of anti-phospholipid syndrome (APS).

Objectives: To investigate the association between aPL antibodies and thromboembolic events, COVID-19 severity, mortality, and cutaneous manifestations in patients with COVID-19.

Methods: aPL antibodies [anti-beta2-glycoprotein-1 (B2GP1) and anti-cardiolipin (aCL) antibodies] were titered in frozen serum samples from hospitalized COVID-19 patients and the patients' clinical records were retrospectively analyzed.

Results: 173 patients were enrolled. aPL antibodies were detected in 34.7% of patients, anti-B2GP1 antibodies in 30.1%, and aCL antibodies in 10.4%. Double positivity was observed in 5.2% of patients. Thromboembolic events occurred in 9.8% of patients, including 11 pulmonary embolisms, 1 case of celiac tripod thrombosis, and six arterial ischemic events affecting the cerebral, celiac, splenic, or femoral-popliteal arteries or the aorta. aPL antibodies were found in 52.9% of patients with vascular events, but thromboembolic events were not correlated to aPL antibodies (adjusted OR = 1.69, p = 0.502). Ten patients (5.8%) had cutaneous signs of vasculopathy: nine livedo reticularis and one acrocyanosis. No significant association was observed between the presence of cutaneous vasculopathy and aPL antibodies (p = 0.692).

Conclusions: Anti-phospholipid antibodies cannot be considered responsible for hypercoagulability and thrombotic events in COVID-19 patients. In COVID-19 patients, livedo reticularis and acrocyanosis do not appear to be cutaneous manifestations of APS.

背景:高凝性是新冠肺炎血栓栓塞事件的危险因素。抗磷脂(aPL)抗体已被假设参与其中。典型的新冠肺炎网状活组织和指部缺血的皮肤学表现可能类似于抗磷脂综合征(APS)的皮肤表现,方法:对新冠肺炎住院患者的冷冻血清样品进行aPL抗体[抗β2-糖蛋白-1(B2GP1)和抗心磷脂(aCL)抗体]的滴度测定,并对患者的临床记录进行回顾性分析。结果:173名患者入选。aPL抗体检出率为34.7%,抗B2GP1抗体检出率30.1%,aCL抗体检出率10.4%,双阳性率5.2%。9.8%的患者发生血栓栓塞事件,包括11例肺栓塞,1例腹腔三脚架血栓形成,以及6例影响脑、腹腔、脾或股腘动脉或主动脉的动脉缺血性事件。在52.9%的血管事件患者中发现了aPL抗体,但血栓栓塞事件与aPL抗体无关(校正OR=1.69,p=0.502)。10名患者(5.8%)有血管病变的皮肤体征:9名网状活组织和1名肢端发绀。皮肤血管病的存在与aPL抗体之间未观察到显著关联(p=0.692)。结论:抗磷脂抗体不能被认为是新冠肺炎患者高凝状态和血栓事件的原因。在新冠肺炎患者中,网织红细胞和肢端发绀似乎不是APS的皮肤表现。
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引用次数: 5
Mannose-binding lectin serum levels and (Gly54asp) gene polymorphism in recurrent aphthous stomatitis: A case-control study. 甘露糖结合凝集素血清水平和(Gly54asp)基因多态性在复发性口疮性口炎中的作用:一项病例对照研究。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211064454
Shereen A Baioumy, Shaimaa H Fouad, Shaimaa A Abdalgeleel, Ahmed A Baiomy, Dina E Sallam, Sara I Taha

Objectives: Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case-control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. Methods: Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. Results: The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545-1320) vs. 1760 ng/mL (1254-2134); p≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively (p =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels (p=0.685) or MBL2 codon 54 genotypes (p=0.382) with the type of ulcers. Conclusion: There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.

目的:免疫反应失调似乎在复发性口疮性口炎(RAS)的发展中起重要作用。本病例对照研究的主要目的是研究RAS患者血液甘露糖结合凝集素(MBL)水平和MBL2基因(gly54asp)多态性的频率,包括40例RAS患者和40例健康对照。方法:采用ELISA法检测血清MBL水平,采用pcr -限制性片段长度多态性法进行MBL2基因分型。结果:RAS组中位血清MBL水平显著低于对照组(975 ng/mL (545-1320) vs. 1760 ng/mL (1254-2134);p≤0.001)。BB基因型的MBL水平显著低于野生AA基因型,中位数分别为525和1340 ng/mL (p =0.005)。B等位基因在RAS患者中的表达比例明显高于对照组。血清MBL水平(p=0.685)和MBL2密码子54基因型(p=0.382)与溃疡类型无显著相关性。结论:血清MBL水平低与MBL2 (gly54asp)基因变异等位基因B及RAS易感性相关。因此,应该研究RAS合并MBL缺乏症患者的潜在新治疗方案。
{"title":"Mannose-binding lectin serum levels and (Gly54asp) gene polymorphism in recurrent aphthous stomatitis: A case-control study.","authors":"Shereen A Baioumy,&nbsp;Shaimaa H Fouad,&nbsp;Shaimaa A Abdalgeleel,&nbsp;Ahmed A Baiomy,&nbsp;Dina E Sallam,&nbsp;Sara I Taha","doi":"10.1177/20587384211064454","DOIUrl":"https://doi.org/10.1177/20587384211064454","url":null,"abstract":"<p><p><b>Objectives:</b> Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case-control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. <b>Methods:</b> Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. <b>Results:</b> The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545-1320) vs. 1760 ng/mL (1254-2134); <i>p</i>≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively (<i>p</i> =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels (<i>p</i>=0.685) or MBL2 codon 54 genotypes (<i>p</i>=0.382) with the type of ulcers. <b>Conclusion:</b> There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.</p>","PeriodicalId":14046,"journal":{"name":"International Journal of Immunopathology and Pharmacology","volume":"35 ","pages":"20587384211064454"},"PeriodicalIF":3.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7f/46/10.1177_20587384211064454.PMC8689634.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39726011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Analysis of human glioma-associated co-inhibitory immune checkpoints in glioma microenvironment and peripheral blood. 胶质瘤微环境和外周血中人胶质瘤相关共抑制免疫检查点分析。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211056505
Shaoping Shen, Qiyan Wu, Jialin Liu, Liangliang Wu, Rong Zhang, Yasushi Uemura, Xinguang Yu, Ling Chen, Tianyi Liu

One biomarker for a better therapeutic effect of immune checkpoint inhibitors is high expression of checkpoint in tumor microenvironment The purpose of this study is to investigate the expression of immune checkpoints in human glioma microenvironment and peripheral blood mononuclear cells. First, single-cell suspension from 20 fresh high-grade glioma (HGG) specimens were obtained, and analyzed for lymphocyte composition, then six co-inhibitory immune checkpoints were analyzed at the same time. Second, 36 PBMC specimens isolated from HGG blood samples were analyzed for the same items. In GME, there were four distinct subtypes of cells, among them, immune cells accounted for an average of 51.3%. The myeloid cell population (CD11b+) was the most common immune cell identified, accounting for 36.14% on average; the remaining were most CD3+CD4+ and CD3+/CD8-/CD4- T lymphocytes. In these cells, we detected the expression of BTLA, LAG3, Tim-3, CTLA-4, and VISTA on varying degrees. While in PBMCs, the result showed that when compared with healthy volunteers, the proportion of NK cells decreased significantly in HGG samples (p < 0.01). Moreover, the expression of BTLA, LAG3, and Tim-3 in CD45+ immune cells in PBMC was more remarkable in glioma samples. In conclusion, the CD11b+ myeloid cells were the predominant immune cells in GME. Moreover, some immune checkpoints displayed a more remarkable expression on the immune cells in GME. And the profile of checkpoint expression in PBMC was partially consistent with that in GME.

免疫检查点抑制剂治疗效果更好的一个生物标志物是肿瘤微环境中检查点的高表达。本研究的目的是研究免疫检查点在人胶质瘤微环境和外周血单个核细胞中的表达。首先,从20个新鲜的高级别胶质瘤(HGG)标本中获得单细胞悬液,分析淋巴细胞组成,然后同时分析6个共抑制免疫检查点。其次,对从HGG血样中分离的36份PBMC标本进行了相同项目的分析。GME中存在4种不同的细胞亚型,其中免疫细胞平均占51.3%。骨髓细胞群(CD11b+)是最常见的免疫细胞群,平均占36.14%;其余以CD3+CD4+和CD3+/CD8-/CD4- T淋巴细胞为主。在这些细胞中,我们检测到不同程度的BTLA、LAG3、Tim-3、CTLA-4和VISTA的表达。而在PBMCs中,结果显示,与健康志愿者相比,HGG样品中NK细胞的比例明显降低(p < 0.01)。在胶质瘤样本中,PBMC中CD45+免疫细胞中BTLA、LAG3和Tim-3的表达更为显著。综上所述,GME以CD11b+髓系细胞为主。此外,一些免疫检查点在GME免疫细胞上的表达更为显著。PBMC的检查点表达谱与GME的部分一致。
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引用次数: 3
Celiac disease: Understandings in diagnostic, nutritional, and medicinal aspects. 乳糜泻:诊断、营养和医学方面的认识。
IF 3.5 3区 医学 Q3 IMMUNOLOGY Pub Date : 2021-01-01 DOI: 10.1177/20587384211008709
Taoufik Ben Houmich, Brahim Admou

Celiac disease (CD) is characterized by clinical polymorphism, with classic, asymptomatic or oligosymptomatic, and extra-intestinal forms, which may lead to diagnostic delay and exposure to serious complications. CD is a multidisciplinary health concern involving general medicine, pediatric, and adult gastroenterology, among other disciplines. Immunology and pathology laboratories have a fundamental role in diagnosing and monitoring CD. The diagnosis consists of serological testing based on IgA anti-transglutaminase (TG2) antibodies combined with IgA quantification to rule out IgA deficiency, a potential misleading factor of CD diagnosis. Positive TG2 serology should be corroborated by anti-endomysium antibody testing before considering an intestinal biopsy. Owing to multiple differential diagnoses, celiac disease cannot be confirmed based on serological positivity alone, nor on isolated villous atrophy. In children with classical signs or even when asymptomatic, with high levels of CD-linked markers and positive HLA DQ2 and/or DQ8 molecules, the current trend is to confirm the diagnosis on basis of the non-systematic use of the biopsy, which remains obligatory in adults. The main challenge in managing CD is the implementation and compliance with a gluten-free diet (GFD). This explains the key role of the dietitian and the active participation of patients and their families throughout the disease-management process. The presence of the gluten in several forms of medicine requires the sensitization of physicians when prescribing, and particularly when dispensing gluten-containing formulations by pharmacists. This underlines the importance of the contribution of the pharmacist in the care of patients with CD within the framework of close collaboration with physicians and nutritionists.

乳糜泻(CD)的特点是临床多态,有典型的、无症状的或少症状的,以及肠道外的形式,这可能导致诊断延迟和暴露于严重的并发症。乳糜泻是一个多学科的健康问题,涉及普通医学、儿科和成人胃肠病学,以及其他学科。免疫学和病理学实验室在诊断和监测乳糜泻中具有基础作用。诊断包括基于IgA抗谷氨酰胺酶(TG2)抗体的血清学检测,结合IgA定量,以排除IgA缺乏,这是乳糜泻诊断的潜在误导因素。在考虑肠活检之前,TG2血清学阳性应通过抗肌内膜抗体检测来证实。由于多种鉴别诊断,乳糜泻不能仅根据血清学阳性或孤立的绒毛萎缩来确诊。对于具有典型体征或无症状的儿童,具有高水平的cd相关标记物和HLA DQ2和/或DQ8分子阳性,目前的趋势是根据非系统使用活检来确认诊断,这在成人中仍然是强制性的。管理乳糜泻的主要挑战是实施和遵守无谷蛋白饮食(GFD)。这解释了在整个疾病管理过程中,营养师和患者及其家属的积极参与的关键作用。在几种形式的药物中存在麸质需要医生在开处方时敏感化,特别是在药剂师分配含麸质的配方时。这强调了药剂师在与医生和营养学家密切合作的框架内对乳糜泻患者护理的贡献的重要性。
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引用次数: 8
期刊
International Journal of Immunopathology and Pharmacology
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