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Anti-Leishmanial drug Pentostam induced histological changes to liver and kidney in male BALB/c wild mice 抗利什曼药物戊妥斯坦诱导雄性BALB/c野生小鼠肝脏和肾脏组织学改变
Pub Date : 2021-01-01 DOI: 10.53626/jimrhs.2021.1202
Nouara Elazirg Elammari, Salem Ramadan Sariti
Leishmaniasis is still a complex disease of (sub) tropical regions of the world caused by Leishmania spp. Antimonial pentostam is an anti-leishmaniasis drug used medically and it is the primary drug employed against leishmaniasis in Libya. It has multiple acute and chronic adverse effects which can be minimized by using the lowest effective dose. This work aimed to investigate the histological changes in the liver and kidneys affected by different doses of pentostam. Adult male of BALB/c wild mice was divided into four groups, 6 mice of each, and i.p. injected with 10mg/kg, 20mg/kg, and 40mg/kg pentostam in addition to a control group. After 28 therapeutic days and finishing the histological procedure to examine the collected tissue specimens, the obtained results of the liver tissue ranged from demonstrating cytoplasmic vacuoles, to hydropic degeneration, focal and hepatocytic necrosis, and lastly irregular area of hepatocytes with condensed pyknotic nuclei (hepatocyte necrosis). As well, the histological examination of kidney tissue ranged between demonstrating mild cloudy swelling (reversible hydropic degeneration), showed stromal aggregates of inflammatory cells (nephritis), and lastly showed renal tubule casts and necrosis. In a final conclusion, there are clear histological changes in the liver and kidneys, which had been seen in this study, which are dose-dependent changes.
利什曼病仍然是由利什曼原虫引起的世界(亚)热带地区的一种复杂疾病,戊托斯坦是一种医学上使用的抗利什曼病药物,也是利比亚用于治疗利什曼病的主要药物。它具有多种急性和慢性不良反应,可以通过使用最低有效剂量来最小化。本研究旨在探讨不同剂量戊妥斯坦对肝脏和肾脏的组织学改变。将BALB/c野生成年雄性小鼠分为4组,每组6只,在对照组的基础上,分别腹腔注射戊戊酸酯10mg/kg、20mg/kg、40mg/kg。治疗28天后,完成组织学检查收集的组织标本,获得的肝组织结果从细胞质空泡,到水变性,局灶性和肝细胞坏死,最后肝细胞不规则区域,核浓缩收缩(肝细胞坏死)。同样,肾组织的组织学检查表现为轻度浑浊肿胀(可逆性水变性),炎症细胞间质聚集(肾炎),最后显示肾小管铸型和坏死。最后的结论是,肝脏和肾脏有明显的组织学改变,这在本研究中已经看到,这是剂量依赖性的变化。
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引用次数: 2
Water Content in Food Drink and IL-10 Level 食品、饮料中水分含量与IL-10水平
Pub Date : 2021-01-01 DOI: 10.53626/jimrhs.2021.1205
R. Lelyana
Introduction: Water affects IL-10 levels in the body. Many people do not understand that water is very important and needed by the body for influencing IL-10 levels. The purpose of writing this paper is to be able to understand the importance of water for the body on IL-10 levels in order to avoid various conditions that interfere with health. Method: Review study. Results: The simplest but most important source of nutrients is water. The water content in the body is about 60-70%, and a loss of about 10% of the water will cause death. Water balance is obtained through food, drink, and the body's metabolic processes. Various components of food and beverages consumed such as coffee drinks, vegetable soups/fruit juices contain water and affect IL-10 levels in the body. Likewise, the water content in bath water and various other daily activities affect IL-10 levels in the body. Discussion: Vegetables, even drinks such as coffee contain water so that they can fulfill the body's health functions, both for skin health, all body organs and body tissues and cells, as well as for metabolic functions and avoiding dehydration conditions. The water content in the food we drink and the water we use greatly affects the condition of the body so that it also affects the body's immune response. This is related to the presence of IL-10 levels where the increase and decrease in IL-10 levels are strongly influenced by the components of the food or drink contained in the water or the water content contained in the food and drink. In fact, bathwater containing sulfur also affects IL-10 levels. So water plays an important role in IL-10 levels in the body. Conclusions: Body health is influenced by water and water also affect IL-10 levels in the body so that it is beneficial for body health related to the immune system.
水会影响体内IL-10的水平。许多人不知道水是非常重要的,身体需要水来影响IL-10的水平。写这篇论文的目的是为了能够理解水对身体IL-10水平的重要性,以避免各种干扰健康的情况。方法:回顾性研究。结果:水是最简单但最重要的营养来源。人体内的含水量约为60-70%,失去10%左右的水分就会导致死亡。水的平衡是通过食物、饮料和身体的代谢过程来实现的。食物和饮料的各种成分,如咖啡饮料、蔬菜汤/果汁,都含有水分,会影响体内IL-10的水平。同样,洗澡水和其他各种日常活动中的水分含量也会影响体内IL-10的水平。讨论:蔬菜,甚至像咖啡这样的饮料都含有水分,这样它们就可以完成身体的健康功能,无论是皮肤健康,身体所有器官和身体组织和细胞,还是代谢功能,避免脱水情况。我们喝的食物和用水中的水分含量对身体的状况影响很大,因此也会影响身体的免疫反应。这与IL-10水平的存在有关,其中IL-10水平的增加和减少受到水中所含食物或饮料成分或食物和饮料中所含含水量的强烈影响。事实上,含硫的洗澡水也会影响IL-10水平。因此,水在体内IL-10水平中起着重要作用。结论:人体健康受水的影响,水也会影响体内IL-10的水平,从而有利于与免疫系统相关的身体健康。
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引用次数: 0
Bioefficacy of local Lantana camara (Verberneae) plant extracts against the 3rd instar larva and adult stages of Anopheles gambiae senso lato (Giles). 马鞭草植物提取物对冈比亚按蚊3龄幼虫和成虫的生物药效研究
Pub Date : 2020-12-13 DOI: 10.15520/jmrhs.v3i12.214
W. Yana, Enda Corinna Andu, K. H. Tofel, A. Henri
Resistance of malaria vectors to synthetic chemicals with high operational cost and environmental pollution has been a great challenge to scientists. Alternative approaches such as the use of natural plant products which are environmentally friendly are put in place to control malaria vectors. This study was focused on testing the effectiveness of three solvent extracts of Lantana camara on the 3rd instar larvae and adults of Anopheles gambiae s. l. These extracts were obtained by maceration. Bioassays test were carried out by WHO’s method for determination of larvicidal and adulticidal efficacy. The results show that, larval mortality increased significantly with the concentration and exposure time. Lethal concentrations 50 (LC50) and 95 (LC95) after 24 hours of larvae exposure time are respectively 0.31 g/mL and 1.53 g/mL  while within 48 h they are 0.27 g/mL and 0.79 g/mL for hexane extract; 1.45 g/mL and 2.0 g/mL (24 h exposure), 0.84 g/mL and 1.55 g/mL (48 h exposure) for acetone extract; 1.96 g/mL and no lethal concentration causing 95% mortality was determined; 0.40 g/mL and 2.20 g/mL (48 h) for aqueous extract. The efficacy of hexane and aqueous extract on the adult knock down and mortality were not significant even with the increasing extract concentrations and exposure time whereas with acetone extract,  the adult LC50 after 24 h was 2.4 g/mL but with 95% mortality lethal concentration was not determined. According to the results, hexane extract showed high larvicidal efficacy of An. gambiae and acetone extract showed significant adult mortality. Those two extracts of L. camara can be used to fight against An. gambiae as alternative malaria vector control to replace conventional insecticides.
疟疾病媒对操作成本高、环境污染大的合成化学品的耐药性一直是科学家面临的巨大挑战。采用诸如使用对环境友好的天然植物产品等替代方法来控制疟疾病媒。采用浸渍法研究了三种溶剂提取物对冈比亚按蚊(Anopheles gambiae s. l) 3龄幼虫和成虫的杀灭效果。采用世界卫生组织的杀虫灭成虫方法进行生物测定试验。结果表明,幼虫死亡率随浓度和暴露时间的增加而显著升高。正己烷提取物暴露24 h后的致死浓度50 (LC50)和95 (LC95)分别为0.31 g/mL和1.53 g/mL,暴露48 h后的致死浓度分别为0.27 g/mL和0.79 g/mL;丙酮提取物为1.45 g/mL和2.0 g/mL(暴露24 h), 0.84 g/mL和1.55 g/mL(暴露48 h);1.96 g/mL,未检测到致95%死亡率的致死浓度;0.40 g/mL, 2.20 g/mL (48 h)。正己烷和水提物对成虫击倒率和死亡率的影响不显著,而丙酮提取物对成虫24 h LC50为2.4 g/mL,但未见95%的致死浓度。结果表明,正己烷提取物具有较高的杀虫效果。冈比亚菌和丙酮提取物显示显著的成虫死亡率。这两种提取物可用于抗安虫病。冈比亚作为替代疟疾媒介控制取代传统杀虫剂。
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引用次数: 2
Essential Treatment of Covid19 Patients covid - 19患者的必要治疗
Pub Date : 2020-12-02 DOI: 10.15520/jmrhs.v3i12.294
Gerry A. Smith
I have recently seen the Italian study of cardiac troponinC in the blood of patients (1).
我最近看到了意大利对患者血液中心肌肌钙蛋白的研究(1)。
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引用次数: 2
The Assessment of Immunomolecular expression and prognostic role of TLR7 among patients with Prostatitis 前列腺炎患者TLR7免疫分子表达及预后评价
Pub Date : 2020-11-07 DOI: 10.15520/jmrhs.v3i11.278
I. Alsaimary, Hussein Naeem Aldhaheri, M. Almusafer
The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected , The molecular expression study include extracting DNA from blood of Prostatitis patients , Prostitis patients and Control group by using specific primers for conventional PCR and Real Time PCR , the amplification of all extracted DNA from blood samples was preform and confirm by using electrophoresis with (100volt/30min).   PCR product was 149bp for TLR7 on agarose gel (1%), (50voltage for 1hour) with a presence 100%. The results of the present study indicate that the Toll like receptor alleles associated with risk of prostatitis.
本研究旨在检测前列腺炎相关toll样受体基因簇的免疫遗传学表达,以了解TLR在男性前列腺炎免疫中的作用。一项病例对照研究包括135例确诊前列腺炎患者和50人作为对照组。收集所有前列腺炎患者的年龄、婚姻状况、工作情况、不孕情况、家族史、感染、过敏、类固醇治疗、居住、吸烟、饮酒、血型、身体最大指数(BMI)等个人信息及临床发现等资料。分子表达研究包括利用特异性引物提取前列腺炎患者、前列腺炎患者和对照组血液中的DNA,进行常规PCR和Real Time PCR;对血样中提取的所有DNA进行扩增,用(100v /30min)电泳确认。TLR7在琼脂糖凝胶(1%)上的PCR产物为149bp,(50电压1小时)存在率为100%。本研究结果提示Toll样受体等位基因与前列腺炎发病风险相关。
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引用次数: 2
The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique. 全自动ELISA技术测定前列腺炎患者前列腺特异性抗原(PSA)浓度。
Pub Date : 2020-11-07 DOI: 10.15520/jmrhs.v3i11.279
Hussein Naeem Aldhaheri, I. Alsaimary, M. Almusafer
      The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.
本研究的目的是检测前列腺炎相关toll样受体基因簇的免疫遗传学表达,以了解TLR在巴士拉和迈桑省男性前列腺炎免疫中的作用。一项病例对照研究包括135例确诊前列腺炎患者和50人作为对照组。收集所有前列腺炎患者的年龄、婚姻状况、工作、不孕、家族史、感染、过敏、类固醇治疗、居住、吸烟、饮酒、血型、身体最大指数(BMI)及临床表现等个人信息。本研究显示PSA水平对前列腺炎患者和对照组的影响,p值<0.0001,因此研究显示PSA水平升高与前列腺炎呈正相关。
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引用次数: 5
Risk Factor of Intra-Uterine Fetal Death in Gaza Governmental Hospitals:Case-Control Study (2018-2019). 加沙政府医院子宫内胎儿死亡的危险因素:病例对照研究(2018-2019)
Pub Date : 2020-10-25 DOI: 10.15520/jmrhs.v3i10.275
A. ElShanti, Bothyna Bassyoni EL syed Etewa, A. Dhair, Naema Mohammed Salem Abo Samra
The study aimed to find out the factors that cause fetal death in the womb in governmental hospitals in Gaza Strip a controlled study during the period 2018-2019. The study problem was formulated in the following main question: What were the most important factors causing childhood deaths in the womb? The study used descriptive and analytical methods. the study sample was (70) women and (70) controls. For each case, one control was matched. The study found that the average age of cases was (29.60) years compared to (27.1) for controls. The most common maternal risk factors for fetal intrauterine death were parity (24.3%) among cases versus (21.4%) among controls. Medication/substance, congenital anomalies of the fetus, bleeding in the third trimester of pregnancy, prolonged pregnancy after birth, and intrauterine growth retardation are the main risk factors. The study recommended conducting a continuing education program for women and nurses about risk factors for fetal death in the womb.
该研究旨在通过2018-2019年的对照研究,找出导致加沙地带政府医院子宫内胎儿死亡的因素。这项研究的主要问题是:导致儿童在子宫内死亡的最重要因素是什么?本研究采用描述性和分析性方法。研究样本为(70)名女性和(70)名对照。对于每个病例,匹配一个对照。研究发现,这些病例的平均年龄为29.60岁,而对照组的平均年龄为27.1岁。胎儿宫内死亡最常见的产妇危险因素是胎次(24.3%),而对照组为(21.4%)。药物/物质、胎儿先天性异常、妊娠晚期出血、产后妊娠时间延长、宫内发育迟缓是主要危险因素。该研究建议对妇女和护士开展继续教育项目,了解子宫内胎儿死亡的危险因素。
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引用次数: 1
SOME WORDS ABOUT FRACTURES OF RADIAL HEAD 关于桡骨头骨折的几点介绍
Pub Date : 2020-10-05 DOI: 10.15520/jmrhs.v3i10.261
B. Saccomanni
A visible posterior fat pad on the lateral view of the elbow is a sign of occult intraarticular pathology. Early elbow ROM is needed to prevent stiffness. If you fixed the fracture, but not well enough to move the elbow, you did not fix it. The “safe zone” for placing hardware on the radial head lies in the interval between the radial styloid and Lister’s tubercle. Examine the wrist when examining all elbow injuries; a radial head fracture may be accompanied by a tear of the interosseous membrane and disruption of the distal radioulnar joint. The posterior interosseous nerve (controlling finger and wrist extension) can be damaged by a radial head injury or by the surgery performed to treat the fracture. Therefore, document functional status preoperatively. The severity of these injuries runs the gamut from minimally displaced fractures needing minimal treatment to those with major displacement or comminution, requiring surgical fixation, excision, or replacement.
肘关节侧面可见后部脂肪垫,是隐匿性关节内病变的征象。需要早期肘关节ROM来防止僵硬。如果你固定了骨折,但没有固定好肘部,你就没有固定好。桡骨头放置硬体的“安全区”位于桡骨茎突和李斯特结节之间的间隙。检查所有肘部损伤时检查手腕;桡骨头骨折可伴有骨间膜撕裂和远端桡尺关节断裂。后骨间神经(控制手指和手腕伸展)可因桡骨头损伤或治疗骨折的手术而受损。因此,术前应记录功能状态。这些损伤的严重程度从需要最少治疗的轻度移位骨折到需要手术固定、切除或置换的严重移位或粉碎骨折不等。
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引用次数: 1
The Effectiveness of Assertiveness Training on Bullying, Competitive State Anxiety and Performance Under Pressure in Futsal Players 自信训练对五人制足球运动员欺凌、竞争状态焦虑和压力下表现的影响
Pub Date : 2020-09-01 DOI: 10.32598/jrh.10.5.21.7.98
Reza Aliyar Najafabadi, Z. Meshkati, R. Badami
The aim of this research was determined the effect of assertiveness training on bullying, competitive state anxiety and performance under pressure in futsal players. 32 futsal players teenage guys who scored from low to moderate were scored in assertiveness questionnaire and were purposefully placed into two experimental and control groups. The experimental group was given eight session training, and no training was given to the control group. The data collection tools were assertiveness, bullying, competitive state anxiety questionnaires and athletes' performance checklist. Analysis of covariance results in bullying, competitive state anxiety and performance under pressure showed a significant difference between the two groups. In general, the results indicate the effect of assertiveness training on the bullying skills, competitive state anxiety, and performance under pressure of futsal teenage players, and suggested that assertiveness training be used to improve the performance under pressure in futsal players.
本研究的目的是确定自信训练对五人制足球运动员恃强凌弱、竞争状态焦虑和压力下表现的影响。对32名得分从低到中等的五人制足球运动员进行自信问卷调查,并有意将其分为实验组和对照组。实验组进行8次训练,对照组不进行训练。数据收集工具为自信、欺凌、竞争状态焦虑问卷和运动员成绩检查表。协方差分析结果显示,霸凌、竞争状态焦虑和压力下表现在两组间存在显著差异。总体而言,研究结果表明了自信训练对五人制足球青少年运动员恃强凌弱技能、竞争状态焦虑和压力下表现的影响,并建议采用自信训练来提高五人制足球运动员的压力下表现。
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引用次数: 4
The Prevalence of Vitamin D Deficiency and Its Related Factors Among Female Staff of Gonabad University of Medical Sciences, Gonabad City, Iran, in 2019 2019年伊朗戈纳巴德市戈纳巴德医科大学女性员工维生素D缺乏症患病率及相关因素调查
Pub Date : 2020-08-10 DOI: 10.32598/JRH.10.5.1783.1
S. Niazi, Abdulghader Taene, M. Kianmehr, A. Abbasnezhad, M. Matlabi
Background: Vitamin D (VitD) plays a significant role in human health, survival, and fertility. It is necessary for calcium absorption and bone growth and the regulation of the immune system. This study aims to determine the prevalence of VitD deficiency and the factors affecting it in women working in the campus of Gonabad University of Medical Sciences, Gonabad City, Iran. Methods: In this cross-sectional analytical study, 70 women working on the campus of Gonabad University of Medical Sciences in 2019 were selected by the census method. After obtaining their consent forms, the researcher-made checklist was completed by the participants. The serum levels of 25-hydroxycholecalciferol (25-OH D3) were the index for measuring VitD. The significance level is set below 0.05. Results: The Mean±SD level of VitD in the research units was 13.67±22.5 ng/mL. About 12.85% of the samples had severe deficiency (less than 10 ng/mL), 32.85% had moderate deficiency (10-20 ng/mL) and 31.42% had mild deficiency (20-30 ng/mL). The prevalence of VitD deficiency was lower in people with a history of physical activity, and those with a history of taking supplements. Also, the highest VitD deficiency was observed in the age group of older than 40 years. Conclusion: According to the results, the prevalence of VitD deficiency in the study population was high because 45.7% of the subjects had severe and moderate VitD deficiency.
背景:维生素D (VitD)在人类健康、生存和生育中起着重要作用。它是钙吸收和骨骼生长以及调节免疫系统所必需的。本研究旨在确定在伊朗戈纳巴德市戈纳巴德医学大学校园工作的妇女中维生素d缺乏症的患病率及其影响因素。方法:采用横断面分析方法,选取2019年在戈纳巴德医科大学校园工作的70名女性为研究对象。在获得他们的同意表格后,由参与者完成研究人员制作的检查表。血清25-羟基胆骨化醇(25-OH D3)水平是测定维生素d的指标。显著性水平设置在0.05以下。结果:各研究单位维生素d平均±SD水平为13.67±22.5 ng/mL。12.85%的样品严重缺乏(小于10 ng/mL), 32.85%的样品中度缺乏(10 ~ 20 ng/mL), 31.42%的样品轻度缺乏(20 ~ 30 ng/mL)。有体育运动史和服用维生素d补充剂史的人群中,维生素d缺乏症的患病率较低。此外,维生素d缺乏症在40岁以上的年龄组中最为严重。结论:研究人群中维生素d缺乏症的患病率较高,45.7%的受试者存在重度和中度维生素d缺乏症。
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引用次数: 0
期刊
International Journal of Medical Research and Health Sciences
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