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Modulation of Physical and Thermal Properties in Wild Banana (Musa balbisiana Colla) Seed Powder by Moisture Variations. 水分变化对野生香蕉(Musa balbisiana Colla)种子粉的物理和热性能的影响
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-22 eCollection Date: 2024-01-01 DOI: 10.1155/2024/8846365
Murlidhar Meghwal, Chitra Lekhwar, Yogesh Kumar, Vivek Kumar, Rajat Suhag, Pramod K Prabhakar

Engineering and flow properties of banana seed powder as a function of moisture content are important for processing, handling, packaging, and transport processes. The bulk density, tapped density, and porosity increased from 377.37 to 427.36 kg m-3, 622.08 to 746.33 kg m-3, and 38.99-43.74%, respectively, within the increasing moisture content range. The Hausner ratio (Hr) and Carr's compressibility index (CI) significantly (p < 0.05) increased with an increase in moisture content (6.16-19.56% db) of banana seed powder, whereas HR fell in the range of 1.4-2.0, indicating cohesive characteristics of banana seed powder. The angle of repose, angle of spatula, and angle of fall exhibited a linear increase, ranging from 40.6° to 49°, 33.4° to 39.4°, and 35.6° to 42.6°, respectively, with increasing moisture content. The static coefficient of friction was found to be highest for aluminium and glass surfaces and least for stainless steel. The water activity and swelling power of banana seed powder showed a significant increase, while the solubility and oil absorption capacity exhibited a significant decrease within the range of increasing moisture content. The thermal characteristics of wild banana seed powder, such as thermal conductivity (0.16 to 0.20 Wm-1 K-1) and volumetric specific heat (0.58 to 0.99 MJm-3 K-1), demonstrated an increasing trend as the moisture content increased. However, the thermal diffusivity showed a decrease from 0.31 to 0.19 (×10-6 m2s-1) with the increase in moisture content.

香蕉籽粉的工程和流动特性与含水量的关系对于加工、处理、包装和运输过程非常重要。在水分含量增加的范围内,香蕉籽粉的体积密度、攻丝密度和孔隙率分别从 377.37 kg m-3 增加到 427.36 kg m-3、622.08 kg m-3 增加到 746.33 kg m-3、38.99%-43.74%。随着香蕉籽粉含水量(6.16-19.56% db)的增加,豪斯纳比率(Hr)和卡尔压缩指数(CI)显著增加(p < 0.05),而豪斯纳比率在 1.4-2.0 范围内下降,表明香蕉籽粉具有内聚特性。随着含水量的增加,静止角、铲角和下落角呈线性增加,分别为 40.6°至 49°、33.4°至 39.4°和 35.6°至 42.6°。研究发现,铝和玻璃表面的静摩擦系数最大,而不锈钢表面的摩擦系数最小。在含水量增加的范围内,香蕉籽粉的水活性和膨胀力显著增加,而溶解度和吸油能力则显著下降。野生香蕉籽粉的热特性,如热导率(0.16 至 0.20 Wm-1 K-1)和体积比热(0.58 至 0.99 MJm-3 K-1),随着含水量的增加呈上升趋势。然而,随着含水量的增加,热扩散率从 0.31 降至 0.19 (×10-6 m2s-1)。
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引用次数: 0
Simultaneous Content Determination of Mono-, Di-, and Fructo-oligosaccharides in Citrus Fruit Juices Using an FTIR-PLS Method Based on Selected Absorption Bands 基于选定吸收带的傅立叶变换红外光谱-PLS 法同时测定柑橘果汁中单糖、双糖和果寡糖的含量
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-10 DOI: 10.1155/2024/9265590
Yurika Yajima, Hideyuki Wakabayashi, K. Suehara, T. Kameoka, Atsushi Hashimoto
A quantification method was developed to determine the sugar components, either following addition or enzymatic treatment, in citrus fruit juices containing additional fructo-oligosaccharides using midinfrared spectroscopy. For the quantification, we compared the results obtained by applying the simultaneous equation method, which uses very little wavenumber information, and the partial least squares (PLS) regression method, which requires a lot of wavenumber information. In order to prevent overfitting in the PLS method, we concentrated on reducing the amount of spectral data used in the analysis. The corresponding FTIR-PLS method led to an accurate quantification of the sugar contents, even in enzymatically treated orange juices with complicated compositions. The spectral data used for model calibration were significantly reduced by focusing on the absorption and assignment information of the sugar components. The RMSEs of Glc, Fru, Suc, GF2, and GF3 in enzyme-treated orange juice before and after spectral data reduction were 0.50, 0.46, 0.61, 0.74, and 0.61 g/L and 0.51, 0.49, 0.73, 0.86, and 0.61 g/L, respectively. The developed method could be easily implemented for practical applications, using a simple measuring instrument since only absorption information at the limited absorption bands is required.
我们开发了一种定量方法,利用中红外光谱仪测定柑橘果汁中添加果寡糖或酶处理后的糖成分。在定量方面,我们比较了使用很少波长信息的同时方程法和需要大量波长信息的偏最小二乘法(PLS)回归法得出的结果。为了防止 PLS 方法的过度拟合,我们集中精力减少了分析中使用的光谱数据量。相应的傅立叶变换红外-PLS 方法可以准确量化糖的含量,即使是经过酶处理的成分复杂的橙汁也不例外。通过关注糖成分的吸收和赋值信息,用于模型校准的光谱数据显著减少。在减少光谱数据前后,酶处理橙汁中 Glc、Fru、Suc、GF2 和 GF3 的均方根误差分别为 0.50、0.46、0.61、0.74 和 0.61 g/L,以及 0.51、0.49、0.73、0.86 和 0.61 g/L。由于只需要有限吸收波段的吸收信息,因此使用简单的测量仪器就可以轻松地将所开发的方法应用于实际应用中。
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引用次数: 0
Analysis of Fatty Acids and Amino Acids of Three Local Freshwater Bagridae Fish Species in the Kampar Kanan River, Indonesia, for Food Security 印度尼西亚甘帕卡南河三种当地淡水鲂鱼的脂肪酸和氨基酸分析,以确保粮食安全
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-04 DOI: 10.1155/2024/6639837
A. Azrita, H. Syandri, H. Zakeri, Harfiandri Damanhuri, N. Aryani
Fish have become an irreplaceable dietary source of animal protein, especially among households with low socioeconomic status in rural and urban areas of Indonesia. This study is aimed at analysing the proximate composition, minerals, fatty acids, and amino acids of three local Bagridae fish species in the Kampar Kanan river, Indonesia. The standard AOAC method was employed to examine the proximate composition of the carcass, and the analysis of amino acids and fatty acids was conducted through HPLC and GC-MS, respectively. The mineral content was determined using AAS. The nutrient composition results of Hemibagrus nemurus, Hemibagrus wyckii, and Mystus nigriceps revealed that the protein content was 24.26%, 22.57%, and 21.39% (% dry weight), whereas the total lipid content was 6.64%, 7.47%, and 7.75%, respectively. Regarding mineral contents, the calcium levels ranged from 1.49 to 1.66 mg/g, iron levels from 28.35 to 40.36 μg/g, and zinc levels from 24.03 to 54.46 μg/g. Among the fatty acids, palmitic acid was the most abundant in all three species, accounting for 25.59–30.70% of the total fatty acids. Additionally, significant amounts of C18:1 (oleic acid), C18:0 (stearic acid), and C20:4 (arachidonic acid) were also detected as primary fatty acids. The calculated atherogenic index values in the three species of Bagridae fish ranged from 0.73 to 0.99, while the thrombogenic index values varied between 0.54 and 0.75. The predominant amino acids found in the three species of Bagridae fish were glutamic acid with their concentrations ranging from 9.10 to 24.34%. These results indicate that consuming the meat of these three freshwater Bagridae fish species caught in the wild does not pose any health risks to consumers. They can be considered a safe and suitable food source with good nutritional quality.
鱼类已成为不可替代的动物蛋白膳食来源,尤其是在印度尼西亚城乡地区社会经济地位较低的家庭中。本研究旨在分析印度尼西亚甘帕卡南河中三种当地袋獾科鱼类的近似成分、矿物质、脂肪酸和氨基酸。采用标准 AOAC 方法检测胴体的近似成分,并分别通过 HPLC 和 GC-MS 分析氨基酸和脂肪酸。矿物质含量采用 AAS 法测定。海马、海马和黑线鲈的营养成分结果显示,蛋白质含量分别为 24.26%、22.57% 和 21.39%(干重百分比),而总脂含量分别为 6.64%、7.47% 和 7.75%。矿物质含量方面,钙含量为 1.49 至 1.66 毫克/克,铁含量为 28.35 至 40.36 微克/克,锌含量为 24.03 至 54.46 微克/克。在三种脂肪酸中,棕榈酸含量最高,占脂肪酸总量的 25.59-30.70%。此外,还检测到大量的 C18:1(油酸)、C18:0(硬脂酸)和 C20:4(花生四烯酸)初级脂肪酸。计算得出的三种袋鲇的动脉粥样硬化指数值在 0.73 至 0.99 之间,而血栓形成指数值在 0.54 至 0.75 之间。在这三种鱼类中发现的主要氨基酸是谷氨酸,其浓度介于 9.10% 至 24.34% 之间。这些结果表明,食用在野外捕获的这三种淡水鱼的肉不会对消费者的健康造成任何危害。它们可被视为安全、合适且营养质量良好的食物来源。
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引用次数: 0
Beta-Carotene Retention and Consumer Acceptability of Selected Products Made from Two Provitamin-A Maize Varieties 两种维生素 A 玉米品种制成的部分产品的β-胡萝卜素保留率和消费者接受度
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-31 DOI: 10.1155/2023/5575291
Victor Munkhuwa, Kingsley Masamba, W. Kasapila
A study was carried out to determine beta-carotene retention and acceptability of selected products made from two provitamin-A maize varieties grown in Malawi, namely, MH43A and MH44A. Beta-carotene retention in the provitamin-A maize products was determined by using a “completely randomised block design” (CRBD) whereby the maize varieties (replicates) were treated as fixed blocks, and similar treatments (analytical tests) were applied in each block. Acceptability of the provitamin-A maize products was determined in 6 to 23-month-old children and their caregivers using a cross-sectional design. Results showed that in three maize products, namely, porridge, maize meal, and fermented maize beverage, there was an increase in beta-carotene, with apparent retentions of 111.13%, 170.27%, and 138.22% for MH43A and 156.50%, 207.13%, and 126.17% for MH44A varieties, respectively. Results on pregerminated maize flour produced from MH43A and MH44A maize varieties showed lower beta-carotene apparent retention values of 63.3% and 84.7%, respectively. Results on acceptability showed that most of the caregivers (47.1%) preferred porridge prepared from MH43A flour compared to porridge prepared from MH44A (30.6%) and the control variety (MH26-white maize) (22.4%). For roasted maize, roasted white maize grains (49.6%) were more preferred compared to the two provitamin-A varieties. Results on acceptability with respect to children showed that most children (63.5% and 53.7%) who tested MH43A and MH44A porridge, respectively, consumed all the porridge. Results from this study have shown that there is a high acceptability of provitamin-A maize porridges amongst children prepared from MH43A (55.5%) and MH44A (51.9%) maize varieties. The study therefore recommends that provitamin-A maize varieties should be promoted through appropriate preparation methods that ensure high beta-carotene retention to address vitamin-A deficiency.
一项研究旨在确定马拉维种植的两个维生素 A 玉米品种(即 MH43A 和 MH44A)制成的精选产品的β-胡萝卜素保留率和可接受性。采用 "完全随机区组设计"(CRBD)确定维生素 A 玉米产品中的β-胡萝卜素保留率,即把玉米品种(重复)作为固定区组处理,每个区组采用类似的处理方法(分析测试)。采用横断面设计,测定 6 至 23 个月大的儿童及其看护人对维生素 A 玉米产品的接受程度。结果表明,在粥、玉米粉和发酵玉米饮料这三种玉米产品中,β-胡萝卜素都有所增加,MH43A品种的表观保留率分别为111.13%、170.27%和138.22%,MH44A品种的表观保留率分别为156.50%、207.13%和126.17%。用 MH43A 和 MH44A 玉米品种生产的预发芽玉米粉的结果显示,β-胡萝卜素表观保留值较低,分别为 63.3% 和 84.7%。可接受性结果显示,与 MH44A(30.6%)和对照品种(MH26-白玉米)(22.4%)相比,大多数护理人员(47.1%)更喜欢用 MH43A 面粉煮粥。至于烤玉米,与两种维生素 A 品种相比,烤白玉米粒(49.6%)更受青睐。关于儿童接受度的结果显示,大多数测试过 MH43A 和 MH44A 粥的儿童(分别为 63.5%和 53.7%)食用了所有粥品。研究结果表明,儿童对用 MH43A(55.5%)和 MH44A(51.9%)玉米品种熬制的维生素 A 玉米粥的接受度很高。因此,研究建议通过适当的制作方法推广维生素 A 玉米品种,确保β-胡萝卜素的高保留率,以解决维生素 A 缺乏问题。
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引用次数: 0
Nutritional and Sensory Evaluation of Yoghurt Incorporated with Unripe False Horn Plantain (Musa paradisiaca var. "apentu"). 酸奶与未成熟的假角蕉(Musa paradisiaca var.)
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-14 eCollection Date: 2023-01-01 DOI: 10.1155/2023/2221302
Bernadine Olivia Leeward, Francis Alemawor, Godwin Deku

Unripe plantain (Musa paradisiaca L.) is rich in nutrients including minerals, vitamin C, and carbohydrates particularly resistant starches with prebiotic properties. However, the fruit is challenged with limited utilisation, and this contributes to its high postharvest losses along the production and supply chain. Information is lacking on incorporating plantain (UPF) in functional dairy food product development. In a completely randomized design, the study evaluated the effect of unripe false horn plantain (var. "apentu") flour (UPF) incorporation (w/v), at 0% (control), 2%, 4%, and 6%, on the composition and sensory quality of yoghurt. The results showed that higher UPF percent incorporation resulted in yoghurts having lower moisture and higher total solid values as well as enhanced nutritional values, in terms of protein, zinc, potassium, calcium, and vitamin C (P < 0.05). Mean pH and total titratable acidity values of the yoghurt products were in the ranges of 3.40-3.65 and 1.00-130%, respectively. Conversely, an increase in UPF incorporation generally reduced consumer likeness scores for yoghurt sensory characteristics including appearance, texture, flavour, taste, aftertaste, and overall acceptability. The control AZ product received the highest ratings in all sensory attributes evaluated. Compared with the control AZ, the BX (2% w/w UPF) yoghurt showed better nutritional quality as well as had comparable ratings for the sensory attributes, particularly in terms of appearance, texture, and flavour. Thus, the formulation containing 2% UPF has the best potential for the production of value-added functional yoghurt, which will be acceptable. However, for high acceptability, further research is needed to improve the impact of UPF incorporation on the overall sensory quality of yoghurt. The study suggests that UPF can serve as a potential supplement for improving the value of yoghurt, and this also contributes to reducing postharvest losses of plantain as a key food security resource. Also, the study findings contribute baseline information to guide future research on functional dairy product development with unripe plantain.

未成熟的车前草(Musa paradisiaca L.)含有丰富的营养成分,包括矿物质、维生素 C 和碳水化合物,特别是具有益生特性的抗性淀粉。然而,车前草果实的利用率有限,这也是其在生产和供应链中收获后损失较高的原因之一。目前还缺乏将车前草(UPF)纳入功能性乳制品开发的相关信息。本研究采用完全随机设计,评估了未熟假角车前草(变种 "apentu")面粉(UPF)添加量(w/v)在 0%(对照组)、2%、4% 和 6% 时对酸奶成分和感官质量的影响。结果表明,UPF 含量越高,酸奶的水分越低、总固形物值越高,蛋白质、锌、钾、钙和维生素 C 的营养价值也越高(P < 0.05)。酸奶产品的平均 pH 值和总滴定酸度值分别在 3.40-3.65 和 1.00-130% 之间。相反,UPF 添加量的增加通常会降低消费者对酸奶感官特征(包括外观、质地、风味、口感、回味和总体可接受性)的好感度评分。对照 AZ 产品在所有感官特性评价中都获得了最高分。与对照 AZ 酸奶相比,BX(含 2% 的 UPF)酸奶的营养质量更好,感官特性的评分也相当,特别是在外观、质地和风味方面。因此,含有 2% UPF 的配方最有潜力生产可被接受的高附加值功能性酸奶。不过,要想获得较高的可接受性,还需要进一步研究提高 UPF 对酸奶整体感官质量的影响。这项研究表明,UPF 可以作为一种潜在的补充剂来提高酸奶的价值,这也有助于减少作为重要粮食安全资源的车前草收获后的损失。此外,研究结果还为指导未来利用未成熟车前草开发功能性乳制品的研究提供了基础信息。
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引用次数: 0
Recording Postprandial Glucose Reactions with Potato Starch Structural Improvements. 用马铃薯淀粉结构改进记录餐后葡萄糖反应
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-13 eCollection Date: 2023-01-01 DOI: 10.1155/2023/1263896
Sadaf Bashir, Zubair Farooq, Sobia Zafar, Tabussam Tufail, Huma Bader Ul Ain, Muzzamal Hussain, Entessar Al Jbawi, Purabi Saha, Roshan Kumar, Richard Owusu Nyarko

The current study is aimed at modifying the structural makeup of potato starch through the application of heat and moisture to better control the postprandial glycemic response. Heat-moisture treatment (HMT) was used to prepare potato starch using different moisture levels and temperatures. The samples were digested with pancreatin to determine the % of easily digestible, slowly digestible, and resistant starch. Subjects were given pudding made with HMT potato starch, and their postprandial glycemic response was tracked by measuring their blood glucose levels. In addition, incremental and total incremental areas under the curve (IAUC, TIAUC) were also assessed. The current findings of in vitro enzymatic digestibility of potato starch showed inconsistent results as measured at different time intervals. Adding moisture and heating the mixture to 30 and 70°C both increased the amount of rapidly digestible starch in all of the treatments from 20 to 40%. The maximum value of slowly digestible starch was 43.63% when the sample was heated to 30°C with a moisture content of 30%. The highest value (68.46%) for resistant starch was achieved at 20% moisture level and 30°C. After eating pudding, blood sugar spiked for the first 60 and 90 min before gradually dropping off over the next 240 min. As a whole, the highest IAUC and TIAUC values, as well as the glycemic index and load, were observed in potato starch heated to 70°C, which contained 40% moisture. Most parameters achieved their highest values when 40% moisture was added and the heat was applied at 70°C.

目前的研究旨在通过加热和加湿来改变马铃薯淀粉的结构组成,从而更好地控制餐后血糖反应。采用不同的水分水平和温度对马铃薯淀粉进行热水分处理(HMT)。用胰蛋白酶消化样品,以确定易消化、慢消化和抗性淀粉的比例。给受试者食用用 HMT 马铃薯淀粉制作的布丁,并通过测量他们的血糖水平跟踪其餐后血糖反应。此外,还评估了增量和总增量曲线下面积(IAUC、TIAUC)。目前对马铃薯淀粉体外酶消化率的研究结果表明,在不同时间间隔测量的结果并不一致。在所有处理中,添加水分和将混合物加热到 30 和 70°C 都会使快速消化淀粉的含量从 20% 增加到 40%。当样品加热到 30°C、水分含量为 30% 时,慢消化淀粉的最大值为 43.63%。抗性淀粉的最高值(68.46%)是在水分含量为 20% 和温度为 30°C 时达到的。吃布丁后,血糖在最初的 60 分钟和 90 分钟内飙升,然后在接下来的 240 分钟内逐渐下降。总体而言,加热至 70°C 且水分含量为 40% 的马铃薯淀粉的 IAUC 和 TIAUC 值最高,血糖生成指数和负荷也最高。添加 40% 的水分并在 70°C 下加热时,大多数参数都达到了最高值。
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引用次数: 0
Investigation on Pomegranate (Punica granatum) Skin as a Potentially Effective Natural Food Preservative 关于石榴皮作为一种潜在有效的天然食品防腐剂的研究
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-04 DOI: 10.1155/2023/1958395
Mohammad Lokman Hossain, Md. Nazim Uddin, T. N. Orchy, S. Naher, M. Begum, Md. Zahurul Haque
The study is aimed at estimating the possibility of using pomegranate peel extracts as a natural food preservative which was investigated in fish samples. All the extracts (cold water, hot water, and 70% methanol) have been tested against five bacteria (Escherichia coli, Staphylococcus aureus, Salmonella Typhi, Bacillus megaterium, and Bacillus subtilis) using the agar disc diffusion method. Hot water extract showed the best result against B. megaterium. The zone of inhibition (ZOI) for hot water extract as crude was taken 20 μl/disc was 25 mm. Total antioxidant activity and total phenolic content (TPC) were determined for different extracts. The results of a test for TPC showed that 70% methanolic extract (MeOH) and cold water extract, respectively, comprised 263.59 mg gallic acid equivalent (GAE)/g and 197.99 mg GAE/g of total phenolics. In addition, MeOH had a total antioxidant activity of 67.55 ± 5.58  mg ascorbic acid equivalent (AAE)/g while hot water extract had a total antioxidant activity of 66.86 ± 3.55  mg AAE/g. The extracts were applied to Ompok pabda fish, and the fish were preserved in prepared extracts for 24 hours in the open air. Total volatile nitrogen (TVN) and sensory parameters were evaluated at regular intervals of 0, 4, 12, 18, and 24 hours. The TVN value of the prepared extracts was found in order of hot water> MeOH> cold water. Fish preserved in hot water extract was in the acceptable range even after 24 hours. Methanol extract and hot water yielded superior outcomes in preserving the fish.
本研究的目的是估计使用石榴皮提取物作为天然食品防腐剂的可能性,并在鱼类样本中进行了研究。所有提取液(冷水、热水、70%甲醇)对大肠杆菌、金黄色葡萄球菌、伤寒沙门氏菌、巨芽孢杆菌、枯草芽孢杆菌5种细菌进行琼脂盘扩散试验。热水浸提液对巨芽孢杆菌的抑菌效果最好。热水浸提物粗提物的抑制带(ZOI)为20 μl/片,为25 mm。测定了不同提取物的总抗氧化活性和总酚含量。TPC试验结果表明,70%甲醇提取物(MeOH)和冷水提取物的总酚含量分别为263.59 mg没食子酸当量(GAE)/g和197.99 mg GAE/g。MeOH的总抗氧化活性为67.55±5.58 mg抗坏血酸当量(AAE)/g,热水提取物的总抗氧化活性为66.86±3.55 mg AAE/g。将提取液涂抹在木豆鱼身上,用制备好的提取液露天保存24小时。每隔0、4、12、18和24小时对总挥发性氮(TVN)和感官参数进行评估。所得提取物的TVN值依次为热水>甲醇>冷水。在热水浸提液中保存24小时的鱼在可接受范围内。甲醇提取物和热水对鱼的保存效果较好。
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引用次数: 0
New Information of the Anatomy and Phytochemical Screening of Pentaclethra macroloba (Willd.) Kuntze (Caesalpinioideae-Leguminosae) Seeds. Pentaclethra macroloba (Willd.) Kuntze (Caesalpinioideae-Leguminosae) 种子解剖和植物化学筛选的新信息。
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI: 10.1155/2023/1446972
Zelina Ataíde Correia, Ely Simone Cajueiro Gurgel, Olivia Ribeiro, Ana Cristina de Andrade Aguiar Dias, Ravendra Kumar, Luis Adriano Santos do Nascimento, Eloisa Helena de Aguiar Andrade, Mozaniel Santana de Oliveira

Pentaclethra macroloba (Willd.), whose common name is "pracaxi," is naturally found in the Amazon region. The present study is aimed at analyzing the anatomy, seed histochemistry, and chemical composition in fatty acid profile of P. macroloba seed oils. Seeds were collected in the cities of Belém, Marituba, and São Domingos do Capim-PA. For the study in light microscopy, scanning electron microscopy, and histochemistry, seeds were sectioned in cross and longitudinal sections of the embryonic axis and fixed in formaldehyde, acetic acid, and 50% ethyl alcohol; neutral-buffered formalin; and formaldehyde and ferrous sulfate and stored in 70% ethyl alcohol. For the anatomical study, the seeds were subjected to the usual techniques of plant anatomy. Histochemical tests were performed on plant material, freehand sectioned, and embedded in histological paraffin with DMSO. The fatty acid profile was determined for gas chromatography (GC-FID). Integument is divided into three strata, monoseriate exotesta, mesotesta formed by several layers of parenchyma cells, and monoseriate endotesta, formed by compressed cells. Cotyledons are composed of thin-walled parenchyma cells with several secretory cavities and secretory idioblasts. The main metabolic classes are lipids, phenolic compounds, carbohydrates, proteins, and alkaloids. The main fatty acids found in P. macroloba oil are oleic, behenic, lignoceric, and linoleic. P. macroloba seeds have important anatomical characteristics for their circumscription in Leguminosae and also in Caesalpinioideae, and their oil is rich in fatty acids essential to the human diet, providing many benefits to the human health, such as fatty acids belonging to the omega family (linoleic, oleic).

Pentaclethra macroloba(Willd.)俗名 "pracaxi",天然存在于亚马逊地区。本研究旨在分析大叶五加科植物种子油的解剖结构、种子组织化学和脂肪酸谱中的化学成分。研究人员在贝伦(Belém)、马里图巴(Marituba)和圣多明戈斯-多卡皮姆(São Domingos do Capim-PA)等城市采集了种子。为了进行光学显微镜、扫描电子显微镜和组织化学研究,对种子进行了胚轴横切面和纵切面切片,并分别用甲醛、醋酸和 50%乙醇、中性缓冲福尔马林、甲醛和硫酸亚铁固定,然后保存在 70% 的乙醇中。在解剖学研究中,种子采用了植物解剖学的常规技术。对植物材料进行组织化学测试,切片后嵌入含二甲基亚砜的组织石蜡中。用气相色谱法(GC-FID)测定脂肪酸谱。内皮分为三层:单层外胚层、由几层实质细胞组成的中胚层和由压缩细胞组成的单层内胚层。子叶由薄壁的实质细胞组成,具有多个分泌腔和分泌型特异细胞。主要代谢物有脂类、酚类化合物、碳水化合物、蛋白质和生物碱。大叶女贞油中的主要脂肪酸是油酸、山嵛酸、木质酸和亚油酸。大叶女贞的种子具有重要的解剖学特征,可被归入豆科(Leguminosae),也可被归入茄科(Caesalpinioideae),其油富含人类饮食所必需的脂肪酸,对人体健康有很多益处,如属于欧米茄家族的脂肪酸(亚油酸、油酸)。
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引用次数: 0
Effect of Ultrasonic-Assisted Enzymatic Hydrolysis on Functional Properties and Antioxidant Activity of Eri Silkworm Pupa Protein Isolate. 超声波辅助酶水解对艾里蚕蛹蛋白分离物功能特性和抗氧化活性的影响
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI: 10.1155/2023/9409710
Kanchana Thongrattanatrai, Rarisara Impaprasert, Worapot Suntornsuk, George Srzednicki

Philosamia ricini (Eri silkworm) pupa protein isolate (EPI) was utilized to prepare pupa protein hydrolysate (EPIH) through enzymatic hydrolysis. Additionally, the isolate underwent ultrasonic treatment at 20 kHz to become ultrasound pretreated EPI (EPIU), which was then enzymatically hydrolyzed to obtain ultrasound pretreated protein hydrolysate (EPIUH). The physicochemical properties of these samples were investigated, including molecular weight, solubility, foaming and emulsion properties, water- and oil-holding capacity, antioxidant activity, and color. When compared to EPI (used as the control), EPIU exhibited a high degree of hydrolysis at 20 minutes (DH = 29.24%). At a total process time of 20 minutes, the degree of hydrolysis for EPIH, EPIU, and EPIUH was found to be 13%, 29%, and 41%, respectively. SDS-PAGE analysis indicated no difference in molecular weight between EPI and EPIU (11-75 kDa). However, the molecular weight profiles of EPIH and EPIUH were reduced (8-45 kDa), resulting in changes in protein functionalities. The high DH value contributed to the enhancement of antioxidant activity, solubility, emulsion capacity, emulsion stability, and foam capacity of the protein isolate at pH 7. Furthermore, the ultrasonic pretreatment of the protein hydrolysate increased the lightness of the protein powder by reducing the enzyme activity of the polyphenol oxidase (PPO). These results suggest that ultrasonic pretreatment of the protein hydrolysate could be applied to improve the properties of Eri silkworm pupa protein for use in the food and beverage industry, such as protein-rich beverages or salad dressings.

利用 Philosamia ricini(艾里蚕)蛹蛋白分离物(EPI)通过酶水解制备蛹蛋白水解物(EPIH)。此外,分离物经过 20 kHz 超声波处理后成为超声波预处理 EPI(EPIU),再经过酶水解得到超声波预处理蛋白水解物(EPIUH)。研究了这些样品的理化特性,包括分子量、溶解度、发泡和乳化特性、持水和持油能力、抗氧化活性和颜色。与 EPI(用作对照)相比,EPIU 在 20 分钟时的水解程度较高(DH = 29.24%)。在总处理时间为 20 分钟时,EPIH、EPIU 和 EPIUH 的水解度分别为 13%、29% 和 41%。SDS-PAGE 分析表明,EPI 和 EPIU(11-75 kDa)的分子量没有差异。然而,EPIH 和 EPIUH 的分子量却降低了(8-45 kDa),导致蛋白质功能发生变化。高 DH 值有助于提高分离蛋白在 pH 值为 7 时的抗氧化活性、可溶性、乳化能力、乳化稳定性和发泡能力。此外,对蛋白水解物进行超声波预处理可降低多酚氧化酶(PPO)的酶活性,从而提高蛋白粉的亮度。这些结果表明,对蛋白水解物进行超声波预处理可改善蚕蛹蛋白的特性,用于食品和饮料行业,如富含蛋白质的饮料或沙拉酱。
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引用次数: 0
Gastrointestinal Effects on the Antioxidant and Immunomodulatory Properties of South African Fynbos Honey. 南非菲恩波斯蜂蜜抗氧化和免疫调节特性的胃肠效应。
IF 4 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-24 eCollection Date: 2023-01-01 DOI: 10.1155/2023/2553197
Innocentia Botlhale Magoshi, Anwani Wendy Nekhumbe, Mohammed Auwal Ibrahim, June Cheptoo Serem, Megan Jean Bester

The Fynbos biome, Western Cape Province, South Africa, produces a unique honey from Apis mellifera capensis. The bioactivity of Fynbos (FB1-FB6) honeys and Manuka, unique manuka factor 15+ (MAN UMF15+) honey subjected to simulated in vitro digestion, was compared. The effect of each phase of digestion on the antioxidant properties and nitric oxide- (NO-) associated immunomodulatory effects was determined. The total phenolic content of MAN (UMF15+) was higher than that of FB honeys, and following digestion, the percentage bioaccessibility (BA) was 68.6% and 87.1 ± 27.0%, respectively. With the Trolox equivalent antioxidant capacity assay, the activity of FB1 and FB6 was similar to MAN (UMF15+) but reduced for FB2, FB3, FB4, and FB5 with a %BA of 77.9% for MAN (UMF15+) and 78.2 ± 13.4% for FB. The oxygen radical absorbance capacity of MAN (UMF15+) and FB honeys was similar and unaltered with digestion. In a cellular environment, using colon adenocarcinoma (Caco-2) cells, both undigested and the gastric digested honey reduced 2,2'-azobis-(2-amidinopropane) dihydrochloride- (AAPH-) mediated peroxyl radical formation. In contrast, following gastroduodenal digestion, the formation of reactive oxygen species (ROS) was increased. In murine macrophage (RAW 264.7) cells, all honeys induced different levels of NO which was significantly increased with digestion for MAN (UMF15+) and FB1. In LPS/IFN-γ stimulated RAW 264.7 macrophages, only undigested MAN (UMF15+) effectively reduced NO levels, and with digestion, NO scavenging activity of MAN (UMF15+) was reduced but increased for FB5 and FB6. In a noncellular environment, MAN (UMF15+), FB1, FB2, and FB6 scavenged NO, and with digestion, this activity was maintained. This study has identified that undigested and gastric-digested FB honey has antioxidant properties with strong potential anticancer effects following gastroduodenal digestion, related to ROS formation. MAN (UMF15+) had anti-inflammatory effects which were lost postdigestion, and in contrast, FB5 and FB6 had anti-inflammatory effects postdigestion.

南非西开普省的Fynbos生物群生产一种独特的蜂蜜,来自蜜蜂。比较了Fynbos (FB1-FB6)蜂蜜和Manuka独特麦卢卡因子15+ (MAN UMF15+)蜂蜜体外模拟消化的生物活性。测定各消化阶段对抗氧化性能和一氧化氮(NO-)相关免疫调节作用的影响。MAN (UMF15+)总酚含量高于FB蜂蜜,经消化后的生物可及度(BA)分别为68.6%和87.1±27.0%。通过Trolox等效抗氧化能力测定,FB1和FB6的活性与MAN (UMF15+)相似,但对FB2、FB3、FB4和FB5的活性降低,MAN (UMF15+)的%BA为77.9%,FB的%BA为78.2±13.4%。MAN (UMF15+)和FB蜂蜜的氧自由基吸收能力相似,且不随消化而改变。在细胞环境中,使用结肠腺癌(Caco-2)细胞,未消化和胃消化的蜂蜜都减少了2,2'-偶氮-(2-氨基丙烷)二盐酸-(AAPH-)介导的过氧自由基形成。相反,胃十二指肠消化后,活性氧(ROS)的形成增加。在小鼠巨噬细胞(RAW 264.7)中,所有蜂蜜都能诱导不同水平的NO,随着MAN (UMF15+)和FB1的消化,NO水平显著升高。在LPS/IFN-γ刺激的RAW 264.7巨噬细胞中,只有未消化的MAN (UMF15+)有效降低NO水平,随着消化,MAN (UMF15+)对FB5和FB6的NO清除活性降低,而增加。在非细胞环境中,MAN (UMF15+), FB1, FB2和FB6清除NO,并且随着消化,这种活性得以维持。本研究发现,未消化和胃消化的FB蜂蜜具有抗氧化特性,在胃十二指肠消化后具有很强的潜在抗癌作用,与ROS形成有关。MAN (UMF15+)具有抗炎作用,但在消化后消失,而FB5和FB6具有抗炎作用。
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引用次数: 0
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International Journal of Food Science
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