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Sorghum and Sorghum-Based Products: Nutritional Composition, Prebiotic Potential and Health Benefits in Gut Microbiota Interactions. 高粱和高粱制品:肠道菌群相互作用中的营养成分、益生元潜力和健康益处。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-28 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/7084868
W M A D Binosha Fernando, Rasheed A Abdulraheem, Kalmee Pramoda Kariyawasam, Amal Sudaraka Samarasinghe, W M Kendrea T Fernando, Hannah Barnes, Malina Robertson, D P W Jayatunga, B G D N K De Silva, Vijay Jayasena

Sorghum (Sorghum bicolor L.) is a member of the Poaceae family and is the fifth most important crop globally. Sorghum grains (SGs) are rich in health-promoting macro- and micronutrients and phytochemicals. SGs are commonly consumed as food or as ingredients in food, especially in African countries. Therefore, food products such as ogi, bread and flour have been and are still being developed from SGs to provide nutritional and health benefits. However, the nutritional and prebiotic potential of SGs, especially the pigment pericarps, has not been fully exploited. This review describes micronutrients in different varieties of sorghum and the health benefits of sorghum consumption, especially its interaction with the human gut microbiota. It further provides a comprehensive update on the properties and health benefits of improved sorghum-based food products. Finally, the influence of processing methods on SGs is summarised.

高粱(Sorghum bicolor L.)是禾本科植物科的一员,是全球第五大作物。高粱谷物(SGs)富含促进健康的宏微量营养素和植物化学物质。SGs通常作为食品或食品成分食用,特别是在非洲国家。因此,诸如ogi、面包和面粉等食品已经并且仍在由SGs开发,以提供营养和健康益处。然而,SGs的营养和益生元潜力,特别是色素果皮,尚未得到充分利用。本文综述了不同品种高粱中的微量营养素以及食用高粱的健康益处,特别是它与人体肠道微生物群的相互作用。它还提供了改进的高粱食品的特性和健康益处的全面更新。最后总结了加工方法对SGs的影响。
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引用次数: 0
Properties of Novel Components of Polysaccharides Isolated From Lycium barbarum Fruits. 枸杞多糖新成分的性质研究。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-28 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/4899439
Yuling Chen, Xueluan Liu, Genglin Lu, Xuan He, Dandan Li, Yunong Tian, Xiaolin Feng, Yong Ye

This study has established structure-function relationships of Lycium barbarum polysaccharides (LBPs) relevant to antioxidant and tyrosinase-inhibitory functions in food systems by integrating optimized extraction, stepwise purification, comprehensive structural analysis, and functional verification, offering a novel multimethod approach to explore structure-based mechanisms of bioactivity. Firstly, hot-water extraction optimized at 30 mL/g, 90°C, and 5 h yielded 7.44% ± 0.17% crude LBPs. Secondly, ion-exchange followed by gel-filtration chromatography separated two purified fractions: LBP-1 (115.1 kDa) and LBP-2 (73.5 kDa). LBP-1 exhibited a compact morphology and featured spectral characteristics indicative of a triple-helix structure, along with both α- and β-glycosidic linkages. In contrast, LBP-2 showed a more amorphous configuration without triple-helix features and predominantly β-linkages. Their monosaccharide profiles also differed, with LBP-1 enriched in glucose, mannuronic acid, galacturonic acid, and glucuronic acid, while LBP-2 contained glucose, galacturonic acid, mannuronic acid, and arabinose. Both fractions exhibited in vitro antioxidant and tyrosinase-inhibitory activities, with LBP-2 demonstrating relatively stronger effects. The enhanced performance of LBP-2 may be associated with its dispersed structure and uronic acid-rich β-linked motifs, which increase the accessibility of functional sites. These insights support the potential application of both LBP-1 and LBP-2 in functional foods, with LBP-2 showing greater promise for use in beverages and fresh-cut fruit products requiring natural antibrowning and antioxidant solutions.

本研究通过优化提取、分步提纯、综合结构分析和功能验证,建立了枸杞多糖在食品体系中抗氧化和酪氨酸酶抑制功能的结构-功能关系,为探索枸杞多糖的结构生物活性机制提供了一种新的多方法方法。首先,热水浸提条件为30 mL/g、90℃、5 h,粗多糖提取率为7.44%±0.17%。其次,离子交换和凝胶过滤层析分离两个纯化组分:LBP-1 (115.1 kDa)和LBP-2 (73.5 kDa)。LBP-1结构紧凑,具有三螺旋结构的光谱特征,同时具有α-和β-糖苷键。相比之下,LBP-2表现出更无定形的结构,没有三螺旋特征,主要是β键。它们的单糖谱也不同,LBP-1富含葡萄糖、甘露糖醛酸、半乳糖醛酸和葡萄糖醛酸,而LBP-2含有葡萄糖、半乳糖醛酸、甘露糖醛酸和阿拉伯糖。两组分均表现出体外抗氧化和酪氨酸酶抑制活性,其中LBP-2的作用较强。LBP-2的性能增强可能与其分散的结构和富含糖醛酸的β连接基序有关,这增加了功能位点的可及性。这些发现支持了LBP-1和LBP-2在功能性食品中的潜在应用,其中LBP-2在需要天然抗褐变和抗氧化溶液的饮料和新鲜水果产品中显示出更大的前景。
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引用次数: 0
Maintenance of the Quality of Coffee (Coffea arabica L.) in Different Packaging and Storage Locations. 咖啡(阿拉比卡咖啡L.)在不同包装和储存地点的品质维持。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-27 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/5049217
Claudia Patricia Gallego, Jenny Pabón, Rubén Darío Medina, Valentina Osorio

The preservation of sensory properties of coffee depends on the quality of the beans, storage conditions, and packaging characteristics. Currently, there is a wide range of packages that preserve bean quality during the postthreshing stages. This research determined the effects of different packaging types and storage conditions on the physical and sensory qualities of green coffee stored for 365 days. Eight packaging materials, consisting of the natural fiber-based materials fique (FN) and paper (P-Mc) and the polyethylene (PE)- and polypropylene (PP)-based materials PE-Max, PE-double, PE-Multi, PP-PVC, PE-EVOH, and PE-PAV, were evaluated in four locations: Manizales (Alto de Letras), Santa Marta, Chinchiná, and a cold room. The temperature and relative humidity in Alto de Letras were constant during the evaluation period, and the sensory quality expressed as the total score SCA (Specialty Coffee Association) was preserved for 365 days in the PE-EVOH, PE-PAV, PE-double, and PP-PVC packages but not in FN. In a broad sense, the SCA score was significantly affected at different storage times, regardless of the kind of packaging for the other locations. In Santa Marta, which is characterized by higher temperature and relative humidity, the SCA score of the samples was affected after 60 days, while in the cold room, the score was affected at 240 days. In Chinchiná, the SCA score decreased throughout the storage time. The environmental storage conditions affected the ability of the different kinds of packaging to preserve coffee quality. Our findings show a new alternative to maintain coffee quality from the farm and during its commercialization chain.

咖啡感官特性的保存取决于咖啡豆的质量、储存条件和包装特性。目前,有各种各样的包装可以在脱粒后阶段保持豆子的质量。本研究确定了不同包装类型和储存条件对保存365天的生咖啡物理和感官品质的影响。八种包装材料,包括天然纤维基材料纤维(FN)和纸(P-Mc)和聚乙烯(PE)和聚丙烯(PP)基材料PE- max, PE-double, PE- multi, PP- pvc, PE- evoh和PE- pav,在四个地点进行了评估:Manizales (Alto de Letras), Santa Marta, chinchin和一个冷室。在评价期间,Alto de Letras的温度和相对湿度保持不变,感官质量(以总分SCA (Specialty Coffee Association)表示)在PE-EVOH、PE-PAV、PE-double和PP-PVC包装中保存了365天,但在FN中没有。从广义上讲,无论其他位置的包装类型如何,SCA分数在不同的存储时间都受到显著影响。在温度和相对湿度较高的圣玛尔塔,样品的SCA评分在60天后受到影响,而在寒冷的房间,样品的SCA评分在240天后受到影响。在chinchina中,SCA评分在整个存储时间内下降。环境储存条件影响不同包装方式对咖啡品质的保存能力。我们的研究结果显示了一种新的选择来保持咖啡的质量从农场和其商业化链。
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引用次数: 0
From Sweetness to Mouthfeel: A Review on Overcoming Sensory Barriers in Sugar-Free Beverages. 从甜味到口感:无糖饮料中克服感官障碍的研究进展。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-22 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/2345722
Imogen Ramsey, Jing Liu, Jan Hendrik Swiegers, Olayide Oladokun

Background: Over recent decades, considerable research has focused on understanding sweet taste mechanisms and developing new noncarbohydrate sweeteners due to rising noncommunicable diseases. New regulations limiting high sugar levels in food and beverages have driven innovation towards reduced-sugar and sugar-free products, often using noncarbohydrate sweeteners. Extensive research was aimed at improving their physical and sensorial properties. However, to the authors' knowledge no publication has taken a holistic approach by integrating all major sensory and functional challenges of sugar reduction in beverages in one structured framework.

Scope and approach: This review addressed these challenges, which included (i) sweetness, (ii) temporal profile in relation to sweetness onset and linger, (iii) masking, (iv) flavour intensity and delivery and (v) mouthfeel enhancement. It firstly discussed the strategy of replacing the sweetness of sugar by utilizing different sweeteners, investigating mechanisms behind the sensory characteristics and solutions and exploring artificial and natural sweeteners and positive allosteric modulators for sweetness. For the temporal profile, methods to measure sweetness onset and linger were investigated. Solutions for masking off-tastes and flavours were discussed, including bitter-blockers, blending of sweeteners, production technologies and flavours with modifying properties. Changes in flavour intensity and delivery were discussed. In the last section, strategies for addressing mouthfeel reduction were summarised including the usage of hydrocolloids, sugar alcohols, fibre syrups and mineral salts.

Key findings and conclusions: Overall, the need for clearer guidelines and fewer restrictions was debated, highlighting conflicting consumer perspectives on the need for healthy, natural and more sustainable products and regulatory barriers around novel solutions. The advancement of scientific knowledge is emphasised, and collaboration between cross-functional teams is discussed as essential to move sugar-free products closer to their sugar-containing counterparts.

背景:近几十年来,由于非传染性疾病的增加,大量的研究集中在理解甜味机制和开发新的非碳水化合物甜味剂上。限制食品和饮料中高糖含量的新法规推动了对低糖和无糖产品的创新,通常使用非碳水化合物甜味剂。广泛的研究旨在改善它们的物理和感官特性。然而,据作者所知,没有出版物采取了一种全面的方法,将饮料中糖减少的所有主要感官和功能挑战整合在一个结构框架中。范围和方法:本综述解决了这些挑战,包括(i)甜味,(ii)与甜味开始和逗留有关的时间特征,(iii)掩蔽,(iv)风味强度和传递以及(v)口感增强。首先讨论了利用不同的甜味剂替代糖的甜味的策略,探讨了感官特征背后的机制和解决方案,并探索了人工和天然甜味剂以及甜味的正变构调节剂。对于时间剖面,研究了测量甜味开始和逗留的方法。讨论了掩盖异味和风味的解决方案,包括苦味阻滞剂、甜味剂的混合、生产技术和具有改性性能的香料。讨论了风味强度和传递的变化。在最后一节中,总结了解决口感减少的策略,包括水胶体,糖醇,纤维糖浆和矿物盐的使用。主要发现和结论:总体而言,就是否需要更明确的准则和更少的限制进行了辩论,突出了消费者对需要健康、自然和更可持续的产品的不同看法,以及在新解决办法方面存在的监管障碍。会议强调了科学知识的进步,并讨论了跨职能团队之间的合作,认为这对于使无糖产品更接近含糖产品至关重要。
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引用次数: 0
Effect of a Biodegradable Cellulose Nanocrystal Film Containing Eryngium planum Extract and Barberry Anthocyanin on the Shelf Life of Rutilus frisii kutum Filets. 含白杨提取物和杨梅花青素的可生物降解纤维素纳米膜对贻贝鱼片保质期的影响。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-21 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/8816376
Behrooz Dast Peyman, Amir Shakerian, Zohreh Mashak, Ebrahim Rahimi, Reza Sharafati Chaleshtori, Swarup Roy

Cellulose nanocrystal/polyvinyl alcohol (PVA) films enriched with Eryngium planum extract (EPE) and barberry anthocyanin (BA) were formulated and assessed for their efficacy in enhancing the shelf life of Rutilus frisii kutum filets under refrigerated storage. High-performance liquid chromatography (HPLC) identified rutin, chlorogenic acid, rosmarinic acid, and kaempferol as the dominant polyphenols in EPE. Structural and morphological characterization using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) revealed successful incorporation and uniform dispersion of bioactives in the nanocellulose-PVA matrix, with nanocomposites displaying spherical morphology (223-508 nm diameter). In addition, a particle size distribution histogram was generated from SEM images to support claims of uniformity. Chemical analyses-pH, thiobarbituric acid reactive substances (TBARSs), and total volatile basic nitrogen (TVB-N)-demonstrated significant preservation of fish quality by films containing EPE and BA, particularly at 3% EPE. Microbiological assessments confirmed reduced bacterial proliferation, with the lowest psychrotrophic, mesophilic, and Enterobacteriaceae counts in 3% EPE-treated samples after 14 days at 4°C. Importantly, treated samples remained below European Union spoilage thresholds (e.g., TVB-N > 25 mg N/100 g and psychrotrophs > 7 log CFU/g) after 14 days, confirming their regulatory relevance. These findings indicate the developed nanocomposite films as promising candidates for active food packaging, significantly extending the shelf life and safety of fish filets.

研究了纤维素纳米晶/聚乙烯醇(PVA)薄膜的制备方法,并对其在冷藏条件下提高贻贝鱼片保质期的效果进行了评价。高效液相色谱法鉴定出芦丁、绿原酸、迷迭香酸和山奈酚为主要多酚类化合物。利用傅里叶变换红外光谱(FTIR)和扫描电镜(SEM)对纳米纤维素-聚乙烯醇基质进行了结构和形态表征,结果表明生物活性物质在纳米纤维素-聚乙烯醇基质中成功结合并均匀分散,纳米复合材料呈球形(直径223-508 nm)。此外,粒度分布直方图由SEM图像生成,以支持均匀性的主张。化学分析——ph值、硫代巴比妥酸活性物质(tbars)和总挥发性碱性氮(TVB-N)——表明,含有EPE和BA的薄膜,特别是在3% EPE的情况下,对鱼的品质有显著的保护作用。微生物学评估证实细菌增殖减少,在4°C下处理14天后,3% epe处理的样品中,嗜冷、嗜中、肠杆菌科细菌数量最低。重要的是,处理后的样品在14天后仍低于欧盟的腐败阈值(例如,TVB-N > 25 mg N/100 g和psychrotrophs > 7 log CFU/g),证实了它们的监管相关性。研究结果表明,所制备的纳米复合膜是活性食品包装的理想选择,可显著延长鱼片的保质期和安全性。
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引用次数: 0
Biopreservation of Ground Beef Patties Using Lactic Acid: A Sustainable Alternative to Synthetic Additives. 用乳酸生物保存碎牛肉饼:合成添加剂的可持续替代品。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-21 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/9930525
Michel M Beya, Michael E Netzel, Yasmina Sultanbawa, Heather E Smyth, Louwrens C Hoffman

The use of synthetic chemicals in meat products has raised consumer health concerns, driving increased interest in natural preservatives. This study evaluated the effectiveness of lactic acid (LA) as a natural preservative in raw beef patties, comparing it to sodium metabisulphite (SMB; 450 ppm) and a negative control (NC). Ground beef was treated with varying concentrations of LA (0.125%, 0.25%, 0.375% and 0.5%) and stored at 4°C ± 1°C for 20 days. Microbial growth, lipid oxidation, pH, colour, cooking yield and texture were analysed throughout the storage period. Results showed that LA-treated patties significantly inhibited microbial growth (p < 0.05) compared to NC, though SMB exhibited the strongest antimicrobial effect. Lipid oxidation levels in LA-treated patties were comparable to NC (p > 0.05), whereas SMB-treated patties had the lowest oxidation values (p < 0.05). Increasing LA concentrations led to a reduction in pH, whilst colour analysis revealed decreased redness and higher metmyoglobin content in LA-treated samples. Texture profile analysis showed no significant differences in hardness, cohesiveness, gumminess or chewiness amongst treatments; however, springiness was affected (p < 0.05). These findings suggest that LA, at concentrations of 0.125% or higher, can effectively extend shelf life, offering a natural alternative to synthetic preservatives like SMB whilst aligning with consumer preferences for cleaner label meat products.

在肉制品中使用合成化学品引起了消费者的健康担忧,促使人们对天然防腐剂的兴趣日益浓厚。本研究评估了乳酸(LA)作为天然防腐剂在生牛肉饼中的有效性,并将其与焦亚硫酸钠(SMB; 450 ppm)和阴性对照(NC)进行了比较。用不同浓度的LA(0.125%、0.25%、0.375%和0.5%)处理碎牛肉,在4°C±1°C下保存20天。在整个储存期间,对微生物生长、脂质氧化、pH值、颜色、蒸煮产量和质地进行了分析。结果表明,与NC相比,la处理的肉饼显著抑制了微生物的生长(p < 0.05),但SMB的抑菌作用最强。la处理肉饼的脂质氧化水平与NC相当(p < 0.05),而smb处理肉饼的氧化值最低(p < 0.05)。增加LA浓度导致pH值降低,而颜色分析显示,在LA处理的样品中,发红减少,肌红蛋白含量增加。质构分析显示,不同处理间的硬度、内聚性、胶性和咀嚼性均无显著差异;但对弹性有影响(p < 0.05)。这些发现表明,浓度为0.125%或更高的LA可以有效延长保质期,为SMB等合成防腐剂提供天然替代品,同时符合消费者对清洁标签肉制品的偏好。
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引用次数: 0
Correction to "Effect of Cowpea and Pumpkin Powders on the Physicofunctional Properties, Total Phenolic Content, Antioxidant Activity, and Consumer Acceptability of Soup". 更正“豇豆和南瓜粉对汤的物理功能特性、总酚含量、抗氧化活性和消费者接受度的影响”。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-20 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/9792486

[This corrects the article DOI: 10.1155/2024/3596783.].

[这更正了文章DOI: 10.1155/2024/3596783]。
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引用次数: 0
Nutritional, Rheological, and Sensory Assessment of Functional Toast Bread Fortified With Rye Flour and Basil Seed Gum Powder. 用黑麦面粉和罗勒籽胶粉强化的功能性烤面包的营养、流变学和感官评价。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-19 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/8841214
Milad Daneshniya, Mohammad Hossein Maleki, Farzaneh Abdolmaleki, Zahra Latifi

The growing demand for functional foods has led to the exploration of alternative ingredients to enhance the nutritional and sensory qualities of bread. This study investigates rye flour (RF) and basil seed gum powder (BSGP) in functional toast bread, evaluating their impact on chemical composition, dough rheology, texture, and sensory properties. BSGP, extracted from basil seeds, was combined with RF at varying concentrations (0%, 15%, and 25% RF and 0%, 0.5%, and 1% BSGP) to produce six different formulations. Chemical analysis revealed that increasing RF and BSGP concentrations significantly enhanced moisture, ash, fat, and fiber content, with the highest concentrations (T6: 25% RF, 1% BSGP) showing the most substantial improvements. Rheological analysis indicated that BSGP reduced pasting temperature and peak viscosity, while higher RF concentrations increased adhesiveness and brittleness over time. Textural evaluations demonstrated that 1% BSGP optimally maintained springiness, while higher RF levels negatively affected texture, resulting in increased brittleness. Sensory analysis identified the combination of 0.5% BSGP and 15% RF (T3) as the most acceptable, balancing taste, texture, and color. Storage studies revealed that Day 1 samples exhibited higher moisture and springiness, whereas Day 5 samples showed increased hardness, brittleness, and adhesiveness, particularly in formulations with higher BSGP levels. Principal component analysis (PCA) captured these temporal trends, emphasizing the relationship between moisture, elasticity, and firmness. In conclusion, RF and BSGP enhance bread's nutritional and sensory properties but require precise formulation to optimize fresh and stored product quality.

对功能性食品日益增长的需求导致了对替代成分的探索,以提高面包的营养和感官品质。本研究研究了功能性烤面包中的黑麦面粉(RF)和罗勒籽胶粉(BSGP),评估了它们对化学成分、面团流变学、质地和感官性能的影响。从罗勒种子中提取的BSGP与不同浓度的RF(0%、15%和25% RF以及0%、0.5%和1% BSGP)结合,得到6种不同的配方。化学分析表明,增加RF和BSGP浓度可显著提高水分、灰分、脂肪和纤维含量,其中最高浓度(T6: 25% RF, 1% BSGP)的改善最为显著。流变学分析表明,BSGP降低了糊化温度和峰值粘度,而随着时间的推移,较高的RF浓度会增加黏附性和脆性。纹理评估表明,1%的BSGP最能保持弹性,而较高的RF水平对纹理产生负面影响,导致脆性增加。感官分析确定0.5% BSGP和15% RF (T3)的组合是最可接受的,平衡了味道,质地和颜色。储存研究表明,第1天的样品表现出更高的水分和弹性,而第5天的样品表现出更高的硬度、脆性和粘性,特别是在含有较高BSGP水平的配方中。主成分分析(PCA)捕捉到这些时间趋势,强调水分、弹性和硬度之间的关系。综上所述,RF和BSGP提高了面包的营养和感官特性,但需要精确的配方来优化新鲜和储存的产品质量。
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引用次数: 0
In Vitro Antiproliferative Activity of Fucoidans Extracted From Sargassum natans and Sargassum fluitans in the HeLa Cell Line. 马尾藻和氟尾藻岩藻胶对HeLa细胞的体外抗增殖活性研究。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-14 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/3001089
S R Vázquez-Rodríguez, A Cavazos-Garduño, C R Cortez-Álvarez, M C Del Toro-Castillo, J C Serrano-Niño

This paper presents an analysis of the polyphenol content, antioxidant capacity, and antiproliferative activity of fucoidans extracted from Sargassum natans and Sargassum fluitans. The results obtained indicated a high total polyphenol content (113 ± 2.3 mg GAE/g), which is probably associated with great antioxidant capacity. Antioxidant activity determined by the DPPH and FRAP methods resulted in 42.21% ± 0.864% inhibition and 1911 ± 71 mM TE/g, respectively. The degree of sulfation for the extracted fucoidans was found to be, using a turbidimetric method with BaCl2, at 13.2% for S. natans and 15.6% for S. fluitans. In addition, intrinsic viscosity determination allowed molecular weights to be estimated using the Mark-Houwink equation as 26.2 and 18.7 kDa for S. natans and S. fluitans, respectively. The antiproliferative properties of fucoidans extracted from Sargassum were evaluated through the MTT assay on HeLa cervical cancer cells. The study revealed a marked reduction in cell proliferation, most notably at higher concentrations (4000 μg/mL), where the inhibition rate reached 90.80%, with an IC50 value of 1277.79 μg/mL. These findings emphasize the strong antiproliferative effects of Sargassum-derived fucoidans, believed to function through pathways such as apoptosis induction, cell cycle modulation, and suppression of cellular growth. The observed biological activity appears strongly tied to structural characteristics like sulfation patterns and molecular weight, which are critical determinants of fucoidan functionality. This aligns with earlier research highlighting the antitumor and antioxidant properties of fucoidans. Moving forward, in vivo studies will be essential to validate their safety profile and therapeutic potential in animal models. Furthermore, detailed mechanistic investigations should be prioritized to uncover the pathways driving their anticancer effects.

本文分析了从马尾藻和马尾藻中提取的岩藻多糖的多酚含量、抗氧化能力和抗增殖活性。结果表明,其总多酚含量(113±2.3 mg GAE/g)较高,可能与较强的抗氧化能力有关。DPPH法和FRAP法测定的抗氧化活性分别为42.21%±0.864%和1911±71 mM TE/g。使用BaCl2浊度法,发现提取的岩藻胶的磺化程度为:S. natans为13.2%,S. fluitans为15.6%。此外,特性粘度测定允许使用Mark-Houwink方程估计S. natans和S. fluitans的分子量分别为26.2和18.7 kDa。采用MTT法观察马尾藻岩藻胶对HeLa宫颈癌细胞的抗增殖作用。实验结果表明,在高浓度(4000 μg/mL)下,其对细胞增殖的抑制率达到90.80%,IC50值为1277.79 μg/mL。这些发现强调了马尾藻衍生的岩藻蛋白具有强大的抗增殖作用,据信其通过诱导凋亡、细胞周期调节和抑制细胞生长等途径发挥作用。观察到的生物活性似乎与结构特征密切相关,如硫酸模式和分子量,这是岩藻糖聚糖功能的关键决定因素。这与早期的研究一致,强调岩藻多糖的抗肿瘤和抗氧化特性。下一步,体内研究对于验证它们在动物模型中的安全性和治疗潜力至关重要。此外,应该优先进行详细的机制研究,以揭示驱动其抗癌作用的途径。
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引用次数: 0
Green Approaches to Enhance Bioactive Compounds in Goji Berry (Lycium barbarum) Fruits: Comparative Optimization of Pressurized Water, Microwave-Assisted, and Ultrasound-Assisted Extraction Technologies by Using Response Surface Methodology. 提高枸杞果实中生物活性物质的绿色途径:利用响应面法对加压水、微波和超声辅助提取技术进行比较优化。
IF 3.1 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-14 eCollection Date: 2025-01-01 DOI: 10.1155/ijfo/8075181
Ece Yildiz-Ozturk

Goji berries (Lycium barbarum L.), a superfruit with a long history of usage in Asian medicine, are gaining recognition for their potential as functional foods because of their high levels of antioxidants, flavonoids, anthocyanins, and phenolic acids. With the growing demand from consumers for clean-label and naturally sourced ingredients, environmentally friendly extraction technologies are now crucial to creating bioactive-rich extracts appropriate for food and nutraceutical applications. Three eco-friendly extraction methods-pressurized water extraction (PWE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE)-are thoroughly evaluated in this study to maximize the bioactive compounds' recovery from Goji berry fruits. Water was the only solvent used in all extraction processes, guaranteeing environmental sustainability and food-grade compliance. The solid/liquid ratio, temperature, duration, pressure, and power were all optimized using response surface methodology (RSM). The total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activity (DPPH inhibition) of the extracted materials were assessed. Under ideal circumstances, the extracts' rutin contents were ascertained by HPLC analysis. According to the findings, MAE had the highest DPPH inhibition rate (75.942%), whereas PWE had the most TPC (17.753 mg GAE/g extract). The flavonoid content of both techniques was comparable. The UAE produced the best energy-to-bioactivity ratio and the most anthocyanin-rich extracts (3.607 mg C3G/g). UAE is the most ecologically friendly option among the techniques, as evidenced by its highest overall efficiency in terms of bioactive recovery and antioxidant capacity. This is the first study to employ a combined approach of RSM and bioactivity-energy efficiency assessment to optimize and compare water-based PWE, MAE, and UAE methods for Goji berries. These results demonstrate that green extraction technologies can be leveraged to sustainably produce bioactive compounds from functional foods like Goji berries, which have significant applications in food, nutraceuticals, and cosmetics.

枸杞(Lycium barbarum L.)是一种在亚洲医学中有着悠久使用历史的超级水果,由于其高水平的抗氧化剂、类黄酮、花青素和酚酸,枸杞作为功能性食品的潜力正在获得认可。随着消费者对清洁标签和天然原料的需求不断增长,环保提取技术现在对于创造适合食品和营养保健应用的富含生物活性的提取物至关重要。为了最大限度地从枸杞果实中提取生物活性物质,本研究对三种环保型提取方法——加压水提取(PWE)、微波辅助提取(MAE)和超声辅助提取(UAE)进行了全面的评价。水是所有提取过程中唯一使用的溶剂,保证了环境的可持续性和食品级的合规性。采用响应面法(RSM)对固液比、温度、持续时间、压力和功率进行优化。测定提取物的总酚含量(TPC)、总黄酮含量(TFC)、总花青素含量(TAC)和抗氧化活性(DPPH抑制)。在理想条件下,用高效液相色谱法测定了芦丁的含量。结果表明,MAE的DPPH抑制率最高(75.942%),而PWE的TPC抑制率最高(17.753 mg GAE/g提取物)。两种方法的类黄酮含量具有可比性。UAE的能量生物活性比最佳,花青素含量最高(3.607 mg C3G/g)。阿联酋是所有技术中最环保的选择,其生物活性恢复和抗氧化能力的整体效率最高。这是第一个采用RSM和生物活性-能源效率评估相结合的方法来优化和比较枸杞的水基PWE、MAE和UAE方法的研究。这些结果表明,绿色提取技术可以用于从枸杞等功能性食品中可持续地生产生物活性化合物,这些功能性食品在食品、保健品和化妆品中有着重要的应用。
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International Journal of Food Science
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