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A modular, human body-mimicking phantom with active thermoregulation capabilities for validation and verification of convective hyperthermia devices. 具有主动体温调节功能的模块化人体模型,用于对流热疗设备的验证和检验。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-11-03 DOI: 10.1080/02656736.2024.2421873
L Van den Bossche, W Vertessen, J Van den Bossche, O Rudenko, J Bogers, L Brancato

Objectives: This study aims to design and fabricate a modular phantom for hyperthermia applications, addressing interpatient variability in thermal regulation mechanisms like sweating rate, metabolic heat production, and blood redistribution.

Materials & methods: The phantom can be constructed in various weights and dimensions by connecting identical units. Each unit consists of an agar-based block, an ethyl cellulose-based top layer, a heat source, deep and superficial water circulation, and a sweating mechanism. Agar and ethyl cellulose gels mimic the thermal properties of human tissues and fat respectively. The blocks are wrapped in PVC foil to prevent water evaporation. A heating wire, coiled around an embedded aluminum tubing simulates metabolic heat production. A superficial water circulation mimics skin capillaries. A water pump ensures a steady flow rate throughout the tubing system. Sweat production is simulated using a water pump and perforated tubing. A programmed controller maintains core temperature in a normal operating mode and simulates an anesthetized patient in anesthesia mode.

Results: Temperature uniformity and regulation were assessed under varying environmental conditions. The phantom effectively regulated its core temperature at 37.0 °C +/- 0.7 °C with an ambient temperature ranging between 21 °C and 30 °C. Activating the water circulation reduced the maximum temperature gradient within the phantom from 4.70 °C to 1.92 °C.

Conclusion: The versatile phantom successfully models heat exchange processes. Its thermal properties, dimensions, and heat exchange rates can be tuned to mimic different patient models. These are promising results as an effective tool for hyperthermia device validation and verification, representing human physiological responses.

目的:本研究旨在设计和制造一个用于热疗应用的模块化模型:本研究旨在设计和制造一种用于热疗应用的模块化模型,解决患者间热调节机制(如出汗率、代谢产热和血液再分布)的差异问题:该模型可通过连接相同的单元来构建不同重量和尺寸的模型。每个单元由琼脂块、乙基纤维素表层、热源、深层和表层水循环以及出汗机制组成。琼脂和乙基纤维素凝胶分别模拟人体组织和脂肪的热特性。实验块用 PVC 箔包裹,以防止水分蒸发。在嵌入的铝管上缠绕着加热丝,模拟新陈代谢产生的热量。表层水循环模拟皮肤毛细血管。水泵可确保整个管道系统保持稳定的流速。使用水泵和穿孔管模拟汗液产生。程序控制器可在正常操作模式下保持核心温度,并在麻醉模式下模拟麻醉病人:结果:在不同环境条件下对温度均匀性和调节进行了评估。在环境温度介于 21 °C 和 30 °C 之间时,模型有效地将核心温度控制在 37.0 °C +/- 0.7 °C。启动水循环后,模型内的最大温度梯度从 4.70°C 降至 1.92°C:多功能模型成功地模拟了热交换过程。它的热特性、尺寸和热交换率可以调整,以模拟不同的病人模型。这些结果很有希望成为热疗设备验证和检验的有效工具,代表人体的生理反应。
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引用次数: 0
Evaluation of acoustic-thermal simulations of in vivo magnetic resonance guided focused ultrasound ablative therapy. 评估体内磁共振引导聚焦超声消融治疗的声热模拟。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-01-17 DOI: 10.1080/02656736.2023.2301489
Nicholas Richards, Douglas Christensen, Joshua Hillyard, Michelle Kline, Sara Johnson, Henrik Odéen, Allison Payne

Purpose: To evaluate numerical simulations of focused ultrasound (FUS) with a rabbit model, comparing simulated heating characteristics with magnetic resonance temperature imaging (MRTI) data collected during in vivo treatment.

Methods: A rabbit model was treated with FUS sonications in the biceps femoris with 3D MRTI collected. Acoustic and thermal properties of the rabbit muscle were determined experimentally. Numerical models of the rabbits were created, and tissue-type-specific properties were assigned. FUS simulations were performed using both the hybrid angular spectrum (HAS) method and k-Wave. Simulated power deposition patterns were converted to temperature maps using a Pennes' bioheat equation-based thermal solver. Agreement of pressure between the simulation techniques and temperature between the simulation and experimental heating was evaluated. Contributions of scattering and absorption attenuation were considered.

Results: Simulated peak pressures derived using the HAS method exceeded the simulated peak pressures from k-Wave by 1.6 ± 2.7%. The location and FWHM of the peak pressure calculated from HAS and k-Wave showed good agreement. When muscle acoustic absorption value in the simulations was adjusted to approximately 54% of the measured attenuation, the average root-mean-squared error between simulated and experimental spatial-average temperature profiles was 0.046 ± 0.019 °C/W. Mean distance between simulated and experimental COTMs was 3.25 ± 1.37 mm. Transverse FWHMs of simulated sonications were smaller than in in vivo sonications. Longitudinal FWHMs were similar.

Conclusions: Presented results demonstrate agreement between HAS and k-Wave simulations and that FUS simulations can accurately predict focal position and heating for in vivo applications in soft tissue.

目的:评估兔模型聚焦超声(FUS)的数值模拟,将模拟加热特性与体内治疗期间收集的磁共振温度成像(MRTI)数据进行比较:对兔子模型的股二头肌进行 FUS 声波治疗,并收集三维 MRTI 数据。实验确定了兔子肌肉的声学和热学特性。创建了兔子的数字模型,并分配了特定组织类型的属性。使用混合角频谱 (HAS) 方法和 k 波进行了 FUS 模拟。使用基于潘尼斯生物热方程的热求解器将模拟的功率沉积模式转换为温度图。评估了模拟技术之间压力的一致性以及模拟和实验加热之间温度的一致性。结果:结果:使用 HAS 方法得出的模拟峰值压力比 k-Wave 模拟峰值压力高出 1.6 ± 2.7%。HAS 和 k-Wave 计算出的峰值压力的位置和 FWHM 显示出良好的一致性。当模拟中的肌肉吸声值调整为测量衰减的 54% 左右时,模拟和实验空间平均温度曲线的平均均方根误差为 0.046 ± 0.019 °C/W。模拟和实验 COTM 之间的平均距离为 3.25 ± 1.37 毫米。模拟声波的横向 FWHM 小于活体声波。结论:上述结果表明,HAS 和 k 波模拟结果一致,FUS 模拟可准确预测软组织体内应用的焦点位置和加热情况。
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引用次数: 0
Microwave ablation for recurrent primary hyperparathyroidism in four patients with multiple endocrine neoplasia type 1: a case series report. 微波消融治疗四例多发性内分泌肿瘤1型患者的复发性原发性甲状旁腺功能亢进症:病例系列报告。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-02-05 DOI: 10.1080/02656736.2024.2308056
Jie Tan, Yuzhi Zhang, Xue Han, Yaofu Fan, Juan Xu, Guofang Chen, Chao Liu, Shuhang Xu

Multiple endocrine neoplasia type 1 (MEN1), a rare tumor syndrome, is inherited in an autosomal dominant pattern, mainly manifested as primary hyperparathyroidism (PHPT). Surgery is preferred for patients with MEN1 and PHPT. Thermal ablation has been widely applied for PHPT but rarely for postoperative recurrent PHPT in MEN1 patients. Based on a series of cases, we aimed to investigate the clinical efficacy and safety of ultrasound-guided percutaneous microwave ablation in the treatment of MEN1 patients with postoperative recurrence of PHPT.

多发性内分泌肿瘤 1 型(MEN1)是一种罕见的肿瘤综合征,为常染色体显性遗传,主要表现为原发性甲状旁腺功能亢进症(PHPT)。MEN1和PHPT患者首选手术治疗。热消融已广泛应用于PHPT,但很少用于MEN1患者术后复发的PHPT。基于一系列病例,我们旨在研究超声引导下经皮微波消融术治疗MEN1患者术后复发PHPT的临床疗效和安全性。
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引用次数: 0
Ultrasound-guided high-intensity focused ultrasound ablation for uterine arteriovenous fistula: a case series. 超声引导下高强度聚焦超声消融治疗子宫动静脉瘘:病例系列。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-03-13 DOI: 10.1080/02656736.2024.2325478
Fang Li, Hua Tao, Jing Chen, Li Wang, Yini Wei, Xiajuan Wu, Yu Zhang, Dingyuan Zeng

Purpose: To evaluate the efficacy and safety of high-intensity focused ultrasound (HIFU) ablation in the treatment of uterine arteriovenous fistula (UAVF).

Materials and methods: This case series included three patients diagnosed with acquired UAVF. All patients underwent routine laboratory tests, electrocardiography (ECG), chest X-ray, ultrasound, and pelvic contrast-enhanced magnetic resonance imaging (MRI). HIFU treatment was performed under sedation and analgesia using a Model JC Focused Ultrasound Tumor Therapeutic System (made by Chongqing Haifu Medical Technology Co. Ltd., China) with a B mode ultrasound device for treatment guidance. The treatment time, sonication power, sonication time, and complications were recorded. Follow-up evaluations were scheduled at 1-, 3-, 6-, and 12-month to assess symptom improvement and evaluate the post-treatment imaging.

Results: All patients completed HIFU treatment in a single session without any major complication. All patients complained of mild lower abdominal and sacrococcygeal pain. Typically, no special treatment is required. Following HIFU treatment, there was a significant relief in clinical symptoms, particularly abnormal uterine bleeding. Ultrasound examinations conducted one month after the treatment revealed a notable reduction in the volume of the lesion, ranging from 57% to 100%. Moreover, the efficacy and safety of HIFU treatment remained consistent during the 12-month follow-up period.

Conclusion: HIFU ablation appears to be an effective and safe treatment modality for UAVF. It provides a noninvasive approach with favorable clinical outcomes.

目的:评估高强度聚焦超声(HIFU)消融术治疗子宫动静脉瘘(UAVF)的有效性和安全性:本病例系列包括三名被诊断为获得性子宫动静脉瘘的患者。所有患者均接受了常规实验室检查、心电图(ECG)、胸部 X 光检查、超声波检查和盆腔造影剂增强磁共振成像(MRI)检查。HIFU 治疗是在镇静和镇痛下进行的,使用的是 JC 型聚焦超声肿瘤治疗系统(中国重庆海扶医疗科技有限公司制造),治疗时使用 B 模式超声设备进行引导。记录治疗时间、超声功率、超声时间和并发症。在1、3、6和12个月时进行随访评估,以评估症状改善情况和治疗后影像学评估:结果:所有患者均在一次治疗中完成了 HIFU 治疗,未出现任何重大并发症。所有患者均主诉有轻微的下腹部和骶尾部疼痛。通常情况下,无需特殊治疗。HIFU 治疗后,临床症状明显缓解,尤其是异常子宫出血。治疗一个月后进行的超声波检查显示,病灶体积明显缩小,缩小幅度从 57% 到 100% 不等。此外,在12个月的随访期间,HIFU治疗的有效性和安全性保持一致:结论:HIFU消融似乎是治疗尿道前列腺增生的一种有效而安全的方法。结论:HIFU消融术是治疗尿道前列腺增生症的一种有效、安全的治疗方法,它是一种无创方法,临床效果良好。
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引用次数: 0
Construction of a 12-gene prognostic model for colorectal cancer based on heat shock protein-related genes. 基于热休克蛋白相关基因构建结直肠癌 12 基因预后模型。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-01-08 DOI: 10.1080/02656736.2023.2290913
Qin Tong, Junchao Zhou

Some heat shock proteins (HSPs) have been shown to influence tumor prognosis, but their prognostic significance in colorectal cancer (CRC) remains unclear. This study explored the prognostic significance of HSP-related genes in CRC. Transcriptional data and clinical information of CRC patients were obtained from The Cancer Genome Atlas (TCGA) database, and a literature search was conducted to identify HSP-related genes. Using Least Absolute Selection and Shrinkage Operator (LASSO) regression and univariate/multivariate Cox regression analyses, 12 HSP-related genes demonstrating significant associations with CRC survival were successfully identified and employed to formulate a predictive risk score model. The efficacy and precision of this model were validated utilizing TCGA and Gene Expression Omnibus (GEO) datasets, demonstrating its reliability in CRC prognosis prediction. gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed significant disparities between high- and low-risk groups in chromatin remodeling biological functions and neutrophil extracellular trap formation pathways. Single sample gene set enrichment analysis (ssGSEA) further revealed differences in immune cell types and immune functional status between the two risk groups. Differential analysis showed higher expression of immune checkpoints within the low-risk group, while the high-risk group exhibited notably higher Tumor Immune Dysfunction and Exclusion (TIDE) scores. Additionally, we predicted the sensitivity of different prognosis risk patients to various drugs, providing potential drug choices for tailored treatment. Combined, our study successfully crafted a novel CRC prognostic model that can effectively predict patient survival, immune landscape, and treatment response, providing important support and guidance for CRC patient prognosis.

一些热休克蛋白(HSP)已被证明可影响肿瘤预后,但它们在结直肠癌(CRC)中的预后意义仍不明确。本研究探讨了 HSP 相关基因在 CRC 中的预后意义。研究人员从癌症基因组图谱(TCGA)数据库中获取了CRC患者的转录数据和临床信息,并进行了文献检索以确定HSP相关基因。利用最小绝对选择和收缩操作器(LASSO)回归和单变量/多变量Cox回归分析,成功鉴定出12个与CRC生存率有显著相关性的HSP相关基因,并利用这些基因建立了一个预测性风险评分模型。基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析表明,高风险组和低风险组在染色质重塑生物功能和中性粒细胞胞外陷阱形成途径方面存在显著差异。单样本基因组富集分析(ssGSEA)进一步揭示了两个风险组在免疫细胞类型和免疫功能状态方面的差异。差异分析表明,低风险组的免疫检查点表达较高,而高风险组的肿瘤免疫功能障碍和排斥(TIDE)评分明显较高。此外,我们还预测了不同预后风险患者对各种药物的敏感性,为定制治疗提供了潜在的药物选择。综上所述,我们的研究成功地建立了一个新型的 CRC 预后模型,该模型能有效预测患者的生存、免疫状况和治疗反应,为 CRC 患者的预后提供了重要的支持和指导。
{"title":"Construction of a 12-gene prognostic model for colorectal cancer based on heat shock protein-related genes.","authors":"Qin Tong, Junchao Zhou","doi":"10.1080/02656736.2023.2290913","DOIUrl":"https://doi.org/10.1080/02656736.2023.2290913","url":null,"abstract":"<p><p>Some heat shock proteins (HSPs) have been shown to influence tumor prognosis, but their prognostic significance in colorectal cancer (CRC) remains unclear. This study explored the prognostic significance of HSP-related genes in CRC. Transcriptional data and clinical information of CRC patients were obtained from The Cancer Genome Atlas (TCGA) database, and a literature search was conducted to identify HSP-related genes. Using Least Absolute Selection and Shrinkage Operator (LASSO) regression and univariate/multivariate Cox regression analyses, 12 HSP-related genes demonstrating significant associations with CRC survival were successfully identified and employed to formulate a predictive risk score model. The efficacy and precision of this model were validated utilizing TCGA and Gene Expression Omnibus (GEO) datasets, demonstrating its reliability in CRC prognosis prediction. gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed significant disparities between high- and low-risk groups in chromatin remodeling biological functions and neutrophil extracellular trap formation pathways. Single sample gene set enrichment analysis (ssGSEA) further revealed differences in immune cell types and immune functional status between the two risk groups. Differential analysis showed higher expression of immune checkpoints within the low-risk group, while the high-risk group exhibited notably higher Tumor Immune Dysfunction and Exclusion (TIDE) scores. Additionally, we predicted the sensitivity of different prognosis risk patients to various drugs, providing potential drug choices for tailored treatment. Combined, our study successfully crafted a novel CRC prognostic model that can effectively predict patient survival, immune landscape, and treatment response, providing important support and guidance for CRC patient prognosis.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139402805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long-term outcome and risk factors of reintervention after high intensity focused ultrasound ablation for uterine fibroids: a systematic review and meta-analysis. 子宫肌瘤高强度聚焦超声消融术后的长期疗效和再次介入的风险因素:系统回顾和荟萃分析。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-01-02 DOI: 10.1080/02656736.2023.2299479
Yuya Dou, Lian Zhang, Yu Liu, Min He, Yanzhou Wang, Zhibiao Wang

Objectives: To quantify the reintervention rate and analyze the risk factors for reintervention after high-intensity focused ultrasound (HIFU) ablation of uterine fibroids.

Methods: Eighteen studies were selected from the seven databases. A meta-analysis was applied to synthesize the reintervention rates for fibroids across various follow-up durations. Subgroup-analysis was conducted based on the year of surgery, sample size, guide methods, and non-perfusion volume ratio (NPVR). Signal intensity of T2-weighted imaging (T2WI) was independently evaluated for reintervention risk.

Results: The study enrolled 5216 patients with fibroids treated with HIFU. There were 3247, 1239, 1762, and 2535 women reaching reintervention rates of 1% (95% confidence interval (CI): 1-1), 7% (95% CI: 4-11), 19% (95% CI: 11-27), and 29% (95% CI: 14-44) at 12, 24, 36, and 60-month after HIFU. The reintervention rates of patients treated with US-guided HIFU (USgHIFU) were significantly lower than those of patients treated with MR-guided focused ultrasound surgery (MRgFUS). When the NPVR of fibroids was over 50%, the reintervention rates at 12, 36 and 60-month after HIFU were 1% (95% CI: 0.3-2), 5% (95% CI: 3-8), and 15% (95% CI: 9-20). The reintervention risk for hyper-intensity fibroids on T2WI was 3.45 times higher (95% CI: 2.7-4.39) for hypo-/iso-intensity fibroids.

Conclusion: This meta-analysis showed that the overall reintervention rates after HIFU were acceptable and provided consultative suggestions regarding treatment alternatives for patients with fibroids. Subgroup-analysis revealed that USgHIFU, NPVR ≥ 50%, and hypo-/iso-intensity of fibroids on T2WI were significant factors in reducing reintervention.

Systematic review registration: PROSPERO, CRD42023456094.

目的量化子宫肌瘤高强度聚焦超声(HIFU)消融术后的再介入率,并分析再介入的风险因素:方法:从七个数据库中筛选出 18 项研究。方法:从 7 个数据库中选取了 18 项研究,采用荟萃分析法对不同随访期的子宫肌瘤再干预率进行综合分析。根据手术年份、样本大小、引导方法和非灌注容积比(NPVR)进行了分组分析。T2加权成像(T2WI)的信号强度是再次介入风险的独立评估指标:研究共纳入了5216名接受HIFU治疗的子宫肌瘤患者。在HIFU治疗后12、24、36和60个月,分别有3247、1239、1762和2535名妇女的再干预率分别为1%(95%置信区间(CI):1-1)、7%(95% CI:4-11)、19%(95% CI:11-27)和29%(95% CI:14-44)。采用 USgHIFU 治疗的患者的再干预率明显低于采用 MRgFUS 治疗的患者。当子宫肌瘤的NPVR超过50%时,HIFU治疗后12个月、36个月和60个月的再干预率分别为1%(95% CI:0.3-2)、5%(95% CI:3-8)和15%(95% CI:9-20)。T2WI上高密度肌瘤的再干预风险是低密度/等密度肌瘤的3.45倍(95% CI:2.7-4.39):这项荟萃分析表明,HIFU术后的总体再介入率是可以接受的,并为子宫肌瘤患者提供了替代治疗的咨询建议。亚组分析显示,USgHIFU、NPVR≥50%以及T2WI上肌瘤的低强度/等强度是减少再干预的重要因素:prospero,CRD42023456094。
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引用次数: 0
Study on the safety and efficacy of HIFU in the treatment of VaIN. 关于 HIFU 治疗 VaIN 的安全性和有效性的研究。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-05-12 DOI: 10.1080/02656736.2024.2346216
Susu Wu, Xiang Li, Dabao Xu, Mingzhu Ye

Purpose: This study aimed to investigate the safety and efficacy of High-Intensity Focused Ultrasound (HIFU) treatment for vaginal intraepithelial neoplasia(VaIN).

Methods: Retrospective analysis was conducted on clinical, pathological, and follow-up data of 43 patients who underwent HIFU treatment for VaIN at Xiangya Third Hospital of Central South University between January 2018 and December 2022. The preliminary efficacy and safety of HIFU in treating VaIN were discussed.

Results: The 36 patients were analyzed, and the average age was 50.09 ± 12.06 years, including 24 patients with VaIN I and 12 patients with VaIN II. Five cases had a history of hysterectomy (4 due to cervical lesions, 1 due to hysteromyoma), and 2 cases had conization of cervical intraepithelial lesions (CIN). All 36 cases were complicated by human papillomavirus (HPV) infection, with 3 cases also having grade I-II CIN and undergoing cervical HIFU treatment. All patients successfully completed the HIFU treatment, with an average treatment time of 5.99 ± 1.25 min, treatment power of 3.5 W, and average total treatment dose of 1118.99 ± 316.20 J. Patients tolerated the treatment well, experiencing only slight pain with VAS score of 3. There was a mild postoperative burning sensation, which resolved within approximately 10-20 min. After 6 follow-up visits, 33 patients (91.66%) achieved cure, 1 patient (2.77%) showed persistence, 2 patients (5.55%) exhibited progression, and 27 patients (75%) tested negative for HPV. At 12 months of follow-up, the results were consistent with those of 6 months. No complications occurred during the procedure and the follow-up period.

Conclusion: HIFU is a safe and effective treatment for VaIN. However, this study had a small sample size, a relatively short follow-up period, and lacked a control group, requiring further investigation.

目的:本研究旨在探讨高强度聚焦超声(HIFU)治疗阴道上皮内瘤变(VaIN)的安全性和有效性:对2018年1月至2022年12月期间在中南大学湘雅三医院接受HIFU治疗的43例VaIN患者的临床、病理及随访资料进行回顾性分析。初步探讨了HIFU治疗VaIN的疗效和安全性:对36例患者进行分析,平均年龄(50.09±12.06)岁,其中VaINⅠ型患者24例,VaINⅡ型患者12例。5例有子宫切除史(4例因宫颈病变,1例因子宫肌瘤),2例有宫颈上皮内病变(CIN)锥切史。所有 36 例患者都合并有人类乳头瘤病毒(HPV)感染,其中 3 例还患有 I-II 级 CIN,并接受了宫颈 HIFU 治疗。所有患者均顺利完成了 HIFU 治疗,平均治疗时间为 5.99 ± 1.25 分钟,治疗功率为 3.5 W,平均治疗总剂量为 1118.99 ± 316.20 J。患者对治疗的耐受性良好,仅有轻微疼痛,VAS 评分为 3 分,术后有轻微烧灼感,约 10-20 分钟内缓解。6 次随访后,33 名患者(91.66%)治愈,1 名患者(2.77%)病情持续,2 名患者(5.55%)病情进展,27 名患者(75%)HPV 检测呈阴性。12 个月的随访结果与 6 个月的结果一致。手术和随访期间均未出现并发症:结论:HIFU 是一种安全有效的 VaIN 治疗方法。结论:HIFU 是治疗 VaIN 的一种安全有效的方法,但这项研究的样本量较小,随访时间相对较短,而且缺乏对照组,因此需要进一步研究。
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引用次数: 0
HMGB1 released from pyroptotic vascular endothelial cells promotes immune disorders in exertional heatstroke. 发热血管内皮细胞释放的 HMGB1 会促进劳累性中暑的免疫紊乱。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-08-08 DOI: 10.1080/02656736.2024.2378867
Chaoping Yu, Yang Huang, Jiangang Xie, Chujun Duan, Shanshou Liu, Wei Zhao, Yutong Wang, Ran Zhuang, Junjie Li, Wen Yin

Background and objective: Exertional heatstroke (EHS) mainly occurs in healthy young people with rapid onset and high mortality. EHS immune disorders can cause systemic inflammatory responses and multiple organ failure; however, the underlying mechanisms remain unclear. As high mobility group box 1 (HMGB1) is a prototypical alarmin that activates inflammatory and immune responses, this study aimed to investigate the effect and mechanism of HMGB1 in the pathogenesis of EHS.

Methods: Peripheral blood mononuclear cell (PBMC) transcriptome sequencing of healthy volunteers, classical heatstroke patients, and EHS patients was performed. A mouse model of EHS was established and murine tissue damage was evaluated by H&E staining. HMGB1 localization and release were visualized using immunofluorescence staining. Human umbilical vein endothelial cells (HUVECs) and THP-1 cells were co-cultured to study the effects of HMGB1 on macrophages. A neutralizing anti-HMGB1 antibody was used to evaluate the efficacy of EHS treatment in mice.

Results: Plasma and serum HMGB1 levels were significantly increased in EHS patients or mice. EHS-induced endothelial cell pyroptosis promoted HMGB1 release in mice. HMGB1 derived from endothelial cell pyroptosis enhanced macrophage pyroptosis, resulting in immune disorders under EHS conditions. Administration of anti-HMGB1 markedly alleviated tissue injury and systemic inflammatory responses after EHS.

Conclusions: The release of HMGB1 from pyroptotic endothelial cells after EHS promotes pyroptosis of macrophages and systemic inflammatory response, and HMGB1-neutralizing antibody therapy has good application prospects for EHS.

背景和目的:劳累性中暑(EHS)主要发生在健康的年轻人身上,发病急、死亡率高。EHS 免疫紊乱可引起全身炎症反应和多器官功能衰竭;然而,其潜在机制仍不清楚。由于高迁移率基团框1(HMGB1)是激活炎症和免疫反应的典型警报蛋白,本研究旨在探讨HMGB1在EHS发病机制中的作用和机制:方法:对健康志愿者、典型中暑患者和 EHS 患者的外周血单核细胞(PBMC)转录组进行测序。建立了 EHS 小鼠模型,并通过 H&E 染色评估了小鼠组织损伤情况。采用免疫荧光染色法观察 HMGB1 的定位和释放情况。为了研究 HMGB1 对巨噬细胞的影响,研究人员将人脐静脉内皮细胞(HUVECs)和 THP-1 细胞进行了联合培养。使用中和抗 HMGB1 抗体评估 EHS 治疗小鼠的疗效:结果:EHS患者或小鼠血浆和血清中的HMGB1水平明显升高。EHS诱导的小鼠内皮细胞热解促进了HMGB1的释放。内皮细胞脓毒症产生的 HMGB1 增强了巨噬细胞的脓毒症,导致 EHS 条件下的免疫紊乱。服用抗 HMGB1 可明显减轻 EHS 后的组织损伤和全身炎症反应:结论:EHS后嗜热内皮细胞释放的HMGB1促进了巨噬细胞的嗜热和全身炎症反应,HMGB1中和抗体疗法在EHS中具有良好的应用前景。
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引用次数: 0
Pilot study on the impact of HIFU treatment on miRNA profiles in vaginal secretions of uterine fibroids and adenomyosis patients. 关于 HIFU 治疗对子宫肌瘤和子宫腺肌症患者阴道分泌物中 miRNA 图谱影响的试点研究。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-10-27 DOI: 10.1080/02656736.2024.2418426
Wei-Chun Chen, Ting-Chang Chang, Lynn Perera, Mei-Hsiu Cheng, Jun-Jie Hong, Chao-Min Cheng

Background: High intensity focused ultrasound (HIFU) ablation treatment for uterine fibroids and adenomyosis has been long developed. The aim of this study is to investigate miRNA profile changes in vaginal secretions after HIFU treatment and their clinical relevance.

Methods: We prospectively collected vaginal secretions samples from 8 patients (1 with adenomyosis and 7 with fibroids) before and after HIFU treatment. RNA was isolated and miRNA profiles were analyzed using next-generation sequencing (NGS) sequencing.

Results: Our study showed miRNA profile change in vaginal secretion samples after HIFU treatment for uterine fibroids/adenomyosis, with 33 miRNAs upregulated and 6 downregulated overall. In fibroid cases, 31 miRNAs were upregulated and 7 downregulated, while in adenomyosis case, 41 miRNAs were upregulated and 71 downregulated. Four miRNAs (hsa-miR-7977, hsa-miR-155-5p, hsa-miR-191-5p, hsa-miR-223-3p) showed significant differences after HIFU treatment in fibroid cases, except in case 5 with the lowest treatment sonications (425 sonications) and energy input (170000 J). hsa-miR-7977 consistently showed downregulation after HIFU treatment. hsa-miR-155-5p were downregulated in case 4 with lowest treatment efficiency (2439.64 J/cm3), while they were upregulated in other cases. hsa-miR-191-5p and hsa-miR-223-3p were downregulated in cases 4 and 7, with case 7 influenced by high sonication and energy due to multiple fibroids.

Conclusions: HIFU treatment altered miRNA profiles in fibroids/adenomyosis patients. Notably, hsa-miR-7977, hsa-miR-155-5p, hsa-miR-191-5p, and hsa-miR-223-3p showed significant changes in fibroid cases, except in low-energy treatments. hsa-miR-7977 consistently decreased post-treatment, while hsa-miR-155-5p decreased in the least efficient cases. Further research is needed for validation.

背景:高强度聚焦超声(HIFU)消融治疗子宫肌瘤和子宫腺肌症已经发展了很长时间。本研究旨在探讨 HIFU 治疗后阴道分泌物中 miRNA 的变化及其临床意义:方法:我们前瞻性地收集了 8 名患者(1 名腺肌症患者和 7 名子宫肌瘤患者)在 HIFU 治疗前后的阴道分泌物样本。方法:我们前瞻性地收集了 8 名患者(1 名腺肌症患者和 7 名子宫肌瘤患者)在 HIFU 治疗前后的阴道分泌物样本,并分离出 RNA,使用新一代测序技术(NGS)分析 miRNA 图谱:结果:我们的研究显示,子宫肌瘤/子宫腺肌症患者在接受 HIFU 治疗后,阴道分泌物样本中的 miRNA 发生了变化,总体上有 33 个 miRNA 上调,6 个下调。在子宫肌瘤病例中,31个miRNA上调,7个下调;在子宫腺肌症病例中,41个miRNA上调,71个下调。在子宫肌瘤病例中,有四个 miRNA(hsa-miR-7977、hsa-miR-155-5p、hsa-miR-191-5p 和 hsa-miR-223-3p)在 HIFU 治疗后出现了显著差异,但治疗超声次数(425 次)和能量输入(170000 焦耳)最低的病例 5 除外。hsa-miR-155-5p在治疗效率最低(2439.64 J/cm3)的病例4中下调,而在其他病例中上调。hsa-miR-191-5p和hsa-miR-223-3p在病例4和7中下调,其中病例7因多发性子宫肌瘤而受到高超声和高能量的影响:结论:HIFU治疗改变了子宫肌瘤/子宫腺肌症患者的miRNA谱。值得注意的是,hsa-miR-7977、hsa-miR-155-5p、hsa-miR-191-5p和hsa-miR-223-3p在子宫肌瘤病例中显示出显著变化,低能量治疗除外。还需要进一步研究验证。
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引用次数: 0
Non-contrast enhanced MRI for efficiency evaluation of high-intensity focused ultrasound in adenomyosis ablation. 非对比增强磁共振成像用于评估高强度聚焦超声在子宫腺肌症消融术中的效率。
IF 3.1 3区 医学 Q2 Medicine Pub Date : 2024-01-01 Epub Date: 2024-01-17 DOI: 10.1080/02656736.2023.2295813
Ma Si, Fajin Lv, Mingmei Tang, Yang Liu, Xueke Qiu, Chunmei Gong, Yan Hu, Yang Liu

Objective: To investigate the value of T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI) in evaluating the therapeutic effect of high-intensity focused ultrasound (HIFU) in adenomyosis ablation.

Material and methods: One hundred eighty-nine patients with adenomyosis were treated with HIFU. The ablation areas on T2WI and DWI sequences were classified into different types: type I, relatively ill-defined rim or unrecognizable; subtype IIa, well-defined rim with hyperintensity; subtype IIb, well-defined rim with hypointensity. The volume of ablation areas on T2WI (VT2WI) and DWI (VDWI) was measured and compared with the non-perfused volume (NPV), and linear regression was conducted to analyze their correlation with NPV.

Results: The VT2WI of type I and type II (subtype IIa and subtype IIb) were statistically different from the corresponding NPV (p = 0.004 and 0.024, respectively), while no significant difference was found between the VDWI of type I and type II with NPV (p = 0.478 and 0.561, respectively). In the linear regression analysis, both VT2WI and VDWI were positively correlated with NPV, with R2 reaching 0.96 and 0.97, respectively.

Conclusions: Both T2WI and DWI have the potential for efficient evaluation of HIFU treatment in adenomyosis, and DWI can be a replacement for CE-T1WI to some extent.

目的研究T2加权成像(T2WI)和弥散加权成像(DWI)在评估高强度聚焦超声(HIFU)消融子宫腺肌症治疗效果中的价值:189 名腺肌症患者接受了 HIFU 治疗。T2WI和DWI序列上的消融区域被分为不同类型:I型,边缘相对不清晰或无法识别;IIa亚型,边缘清晰但密度过高;IIb亚型,边缘清晰但密度过低。测量 T2WI(VT2WI)和 DWI(VDWI)上消融区域的体积,并将其与非灌注体积(NPV)进行比较,同时进行线性回归分析它们与 NPV 的相关性:结果:Ⅰ型和Ⅱ型(Ⅱa亚型和Ⅱb亚型)的VT2WI与相应的NPV有统计学差异(p分别为0.004和0.024),而Ⅰ型和Ⅱ型的VDWI与NPV无显著差异(p分别为0.478和0.561)。在线性回归分析中,VT2WI 和 VDWI 均与 NPV 呈正相关,R2 分别达到 0.96 和 0.97:T2WI和DWI都有潜力对子宫腺肌症的HIFU治疗进行有效评估,DWI在一定程度上可以替代CE-T1WI。
{"title":"Non-contrast enhanced MRI for efficiency evaluation of high-intensity focused ultrasound in adenomyosis ablation.","authors":"Ma Si, Fajin Lv, Mingmei Tang, Yang Liu, Xueke Qiu, Chunmei Gong, Yan Hu, Yang Liu","doi":"10.1080/02656736.2023.2295813","DOIUrl":"10.1080/02656736.2023.2295813","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the value of T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI) in evaluating the therapeutic effect of high-intensity focused ultrasound (HIFU) in adenomyosis ablation.</p><p><strong>Material and methods: </strong>One hundred eighty-nine patients with adenomyosis were treated with HIFU. The ablation areas on T2WI and DWI sequences were classified into different types: type I, relatively ill-defined rim or unrecognizable; subtype IIa, well-defined rim with hyperintensity; subtype IIb, well-defined rim with hypointensity. The volume of ablation areas on T2WI (V<sub>T2WI</sub>) and DWI (V<sub>DWI</sub>) was measured and compared with the non-perfused volume (NPV), and linear regression was conducted to analyze their correlation with NPV.</p><p><strong>Results: </strong>The V<sub>T2WI</sub> of type I and type II (subtype IIa and subtype IIb) were statistically different from the corresponding NPV (<i>p</i> = 0.004 and 0.024, respectively), while no significant difference was found between the V<sub>DWI</sub> of type I and type II with NPV (<i>p</i> = 0.478 and 0.561, respectively). In the linear regression analysis, both V<sub>T2WI</sub> and V<sub>DWI</sub> were positively correlated with NPV, with <i>R</i><sup>2</sup> reaching 0.96 and 0.97, respectively.</p><p><strong>Conclusions: </strong>Both T2WI and DWI have the potential for efficient evaluation of HIFU treatment in adenomyosis, and DWI can be a replacement for CE-T1WI to some extent.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International Journal of Hyperthermia
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