首页 > 最新文献

International Journal of Hyperthermia最新文献

英文 中文
Theoretical evaluation of the impact of diverse treatment conditions by calculation of the tumor control probability (TCP) of simulated cervical cancer Hyperthermia-Radiotherapy (HT-RT) treatments in-silico. 通过计算模拟宫颈癌热疗-放疗(HT-RT)治疗的肿瘤控制概率(TCP),对不同治疗条件的影响进行理论评估。
IF 3.1 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-03-11 DOI: 10.1080/02656736.2024.2320852
Sergio Mingo Barba, Adela Ademaj, Dietmar Marder, Oliver Riesterer, Marco Lattuada, Rudolf M Füchslin, Alke Petri-Fink, Stephan Scheidegger

Introduction: Hyperthermia (HT) induces various cellular biological processes, such as repair impairment and direct HT cell killing. In this context, in-silico biophysical models that translate deviations in the treatment conditions into clinical outcome variations may be used to study the extent of such processes and their influence on combined hyperthermia plus radiotherapy (HT + RT) treatments under varying conditions.

Methods: An extended linear-quadratic model calibrated for SiHa and HeLa cell lines (cervical cancer) was used to theoretically study the impact of varying HT treatment conditions on radiosensitization and direct HT cell killing effect. Simulated patients were generated to compute the Tumor Control Probability (TCP) under different HT conditions (number of HT sessions, temperature and time interval), which were randomly selected within margins based on reported patient data.

Results: Under the studied conditions, model-based simulations suggested a treatment improvement with a total CEM43 thermal dose of approximately 10 min. Additionally, for a given thermal dose, TCP increased with the number of HT sessions. Furthermore, in the simulations, we showed that the TCP dependence on the temperature/time interval is more correlated with the mean value than with the minimum/maximum value and that comparing the treatment outcome with the mean temperature can be an excellent strategy for studying the time interval effect.

Conclusion: The use of thermoradiobiological models allows us to theoretically study the impact of varying thermal conditions on HT + RT treatment outcomes. This approach can be used to optimize HT treatments, design clinical trials, and interpret patient data.

导言热疗(HT)会诱发各种细胞生物学过程,如修复受损和直接杀伤热疗细胞。在这种情况下,将治疗条件的偏差转化为临床结果变化的in-silico生物物理模型可用于研究这些过程的程度及其在不同条件下对热疗+放疗(HT + RT)联合治疗的影响:方法:使用针对 SiHa 和 HeLa 细胞系(宫颈癌)校准的扩展线性二次模型,从理论上研究不同热疗治疗条件对放射增敏和直接热疗细胞杀伤效应的影响。根据报告的患者数据,在边际范围内随机选择患者,生成模拟患者,计算不同热疗条件(热疗次数、温度和时间间隔)下的肿瘤控制概率(TCP):结果:在所研究的条件下,基于模型的模拟结果表明,CEM43的总热剂量约为10分钟,治疗效果有所改善。此外,在给定的热剂量下,TCP 随 HT 治疗次数的增加而增加。此外,在模拟中,我们发现 TCP 对温度/时间间隔的依赖性与平均值的相关性比与最小/最大值的相关性更高,将治疗结果与平均温度进行比较是研究时间间隔效应的绝佳策略:热放射生物学模型的使用使我们能够从理论上研究不同热条件对 HT + RT 治疗效果的影响。这种方法可用于优化热疗治疗、设计临床试验和解释患者数据。
{"title":"Theoretical evaluation of the impact of diverse treatment conditions by calculation of the tumor control probability (TCP) of simulated cervical cancer Hyperthermia-Radiotherapy (HT-RT) treatments in-silico.","authors":"Sergio Mingo Barba, Adela Ademaj, Dietmar Marder, Oliver Riesterer, Marco Lattuada, Rudolf M Füchslin, Alke Petri-Fink, Stephan Scheidegger","doi":"10.1080/02656736.2024.2320852","DOIUrl":"10.1080/02656736.2024.2320852","url":null,"abstract":"<p><strong>Introduction: </strong>Hyperthermia (HT) induces various cellular biological processes, such as repair impairment and direct HT cell killing. In this context, <i>in-silico</i> biophysical models that translate deviations in the treatment conditions into clinical outcome variations may be used to study the extent of such processes and their influence on combined hyperthermia plus radiotherapy (HT + RT) treatments under varying conditions.</p><p><strong>Methods: </strong>An extended linear-quadratic model calibrated for SiHa and HeLa cell lines (cervical cancer) was used to theoretically study the impact of varying HT treatment conditions on radiosensitization and direct HT cell killing effect. Simulated patients were generated to compute the Tumor Control Probability (TCP) under different HT conditions (number of HT sessions, temperature and time interval), which were randomly selected within margins based on reported patient data.</p><p><strong>Results: </strong>Under the studied conditions, model-based simulations suggested a treatment improvement with a total CEM43 thermal dose of approximately 10 min. Additionally, for a given thermal dose, TCP increased with the number of HT sessions. Furthermore, in the simulations, we showed that the TCP dependence on the temperature/time interval is more correlated with the mean value than with the minimum/maximum value and that comparing the treatment outcome with the mean temperature can be an excellent strategy for studying the time interval effect.</p><p><strong>Conclusion: </strong>The use of thermoradiobiological models allows us to theoretically study the impact of varying thermal conditions on HT + RT treatment outcomes. This approach can be used to optimize HT treatments, design clinical trials, and interpret patient data.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2320852"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140093900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Safety and efficacy of indocyanine green fluorescence imaging for real-time guidance of laparoscopic thermal ablation in patients with liver cancer. 吲哚菁绿荧光成像用于实时引导肝癌患者腹腔镜热消融的安全性和有效性。
IF 3.1 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-02-25 DOI: 10.1080/02656736.2024.2306818
Qitao Hu, Zhou Tian, Yongji Sun, Bo Zhang, Zhe Tang

Purpose: To evaluate the safety and efficacy of indocyanine green fluorescence imaging for real-time guidance of laparoscopic thermal ablation in patients with liver cancer.

Materials and methods: A total of 27 patients with 40 liver lesions underwent fluorescence-assisted laparoscopic ablation between January 2020 to March 2023. The sensitivity of indocyanine green (ICG)-fluorescence imaging, technique effectiveness rate and complications of fluorescence-assisted laparoscopic thermal ablation were evaluated.

Results: In total, 33 out of the 40 lesions were identified by ICG-fluorescence imaging technique, with the sensitivity of 82.5%. The sensitivity of ICG-fluorescence imaging of tumor detection in liver surface of parenchyma was significantly higher than that in the deeply located hepatic parenchyma (96.8% vs 33.3%, p = 0.002). ICG-fluorescence imaging procedures detected 4 lesions that cannot be seen on intraoperative ultrasound. It provides clear demarcation lines on the hepatic surface. Technical success is achieved if the necrotic zone had at least a 5 mm ablative margin around the outer edge of the ICG-fluorescence image. Technical success of fluorescence laparoscopic radiofrequency ablation (FLRFA) and fluorescence laparoscopic microwave ablation (FLMWA) was 100% (27/27). Technical effectiveness is defined by the complete necrotic lesions of the local tumor tissue during follow-up. According to the CT/MRI one month after FLRFA or FLMWA, the technical efficacy rate was 92.5% (37/40) and local tumor progression occurred in 7.5% (3/40) of the enrolled lesions. During the follow-up period, no major complications were observed.

Conclusion: ICG-fluorescence imaging guided laparoscopic thermal ablation was feasible, safe and effective.

目的:评估吲哚菁绿荧光成像用于实时引导肝癌患者腹腔镜热消融术的安全性和有效性:2020年1月至2023年3月期间,共有27例40个肝脏病灶的患者接受了荧光辅助腹腔镜消融术。对荧光辅助腹腔镜热消融术的吲哚菁绿(ICG)荧光成像灵敏度、技术有效率和并发症进行了评估:结果:在 40 个病灶中,共有 33 个通过 ICG 荧光成像技术识别,灵敏度为 82.5%。ICG荧光成像检测肝实质表面肿瘤的灵敏度(96.8% vs 33.3%,P = 0.002)明显高于肝实质深部的灵敏度。ICG荧光成像程序发现了4个术中超声检查看不到的病灶。它在肝脏表面提供了清晰的分界线。如果坏死区在ICG荧光成像外缘至少有5毫米的消融边缘,则技术成功。荧光腹腔镜射频消融术(FLRFA)和荧光腹腔镜微波消融术(FLMWA)的技术成功率为100%(27/27)。技术有效性的定义是随访期间局部肿瘤组织完全坏死。根据FLRFA或FLMWA术后一个月的CT/MRI检查结果,技术有效率为92.5%(37/40),7.5%(3/40)的入选病灶出现局部肿瘤进展。在随访期间,未发现重大并发症:ICG荧光成像引导的腹腔镜热消融术是可行、安全和有效的。
{"title":"Safety and efficacy of indocyanine green fluorescence imaging for real-time guidance of laparoscopic thermal ablation in patients with liver cancer.","authors":"Qitao Hu, Zhou Tian, Yongji Sun, Bo Zhang, Zhe Tang","doi":"10.1080/02656736.2024.2306818","DOIUrl":"https://doi.org/10.1080/02656736.2024.2306818","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the safety and efficacy of indocyanine green fluorescence imaging for real-time guidance of laparoscopic thermal ablation in patients with liver cancer.</p><p><strong>Materials and methods: </strong>A total of 27 patients with 40 liver lesions underwent fluorescence-assisted laparoscopic ablation between January 2020 to March 2023. The sensitivity of indocyanine green (ICG)-fluorescence imaging, technique effectiveness rate and complications of fluorescence-assisted laparoscopic thermal ablation were evaluated.</p><p><strong>Results: </strong>In total, 33 out of the 40 lesions were identified by ICG-fluorescence imaging technique, with the sensitivity of 82.5%. The sensitivity of ICG-fluorescence imaging of tumor detection in liver surface of parenchyma was significantly higher than that in the deeply located hepatic parenchyma (96.8% vs 33.3%, <i>p</i> = 0.002). ICG-fluorescence imaging procedures detected 4 lesions that cannot be seen on intraoperative ultrasound. It provides clear demarcation lines on the hepatic surface. Technical success is achieved if the necrotic zone had at least a 5 mm ablative margin around the outer edge of the ICG-fluorescence image. Technical success of fluorescence laparoscopic radiofrequency ablation (FLRFA) and fluorescence laparoscopic microwave ablation (FLMWA) was 100% (27/27). Technical effectiveness is defined by the complete necrotic lesions of the local tumor tissue during follow-up. According to the CT/MRI one month after FLRFA or FLMWA, the technical efficacy rate was 92.5% (37/40) and local tumor progression occurred in 7.5% (3/40) of the enrolled lesions. During the follow-up period, no major complications were observed.</p><p><strong>Conclusion: </strong>ICG-fluorescence imaging guided laparoscopic thermal ablation was feasible, safe and effective.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2306818"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139971801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating the optimal maximum diameter of benign thyroid nodules for thermal ablation on the basis of complete disappearance rate. 以完全消失率为基础,研究热消融良性甲状腺结节的最佳最大直径。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-09-26 DOI: 10.1080/02656736.2024.2408374
Shi-Liang Cao, Wan-Ying Shi, Zhen-Long Zhao, Ying Wei, Na Yu, Jie Wu, Li-Li Peng, Yan Li, Ming-An Yu

Objective: Thermal ablation (TA) is a safe and effective treatment for benign thyroid nodules (BTNs). However, there has been no consensus on the optimal maximum diameter (MD) of BTNs for TA. This study aimed to identify the optimal MD of BTNs for TA based on complete disappearance rate after TA.

Materials and methods: This retrospective study included 639 BTNs treated with TA from June 2014 to January 2022. The complete disappearance rate of BTNs after TA was summarized, related influencing factors were explored, and the optimal MD of BTNs for TA was identified.

Results: At the final follow-up (median: 40 months, range: 24-95 months), the overall volume reduction rate was 95.4 ± 9.0%, and 50.5% of the BTNs (323/639) completely disappeared. The MD was significantly negatively correlated with complete disappearance (odds ratio 0.89, 95% confidence interval 0.87-0.92; p < 0.001). Calcification, comet-tail artifacts, multilocular cysts, and composition of BTNs, as well as diabetes were negatively correlated with complete disappearance. Restricted cubic spline indicated that an MD of 25.0 mm was the optimal threshold of BTNs for TA, which was confirmed by subgroup logistic regression analysis. Compared with BTNs with MD ≤ 25.0 mm, those with MD > 25.0 mm had a greater complication rate (6.5% vs. 2.4%, p = 0.012).

Conclusions: The MD of BTNs was negatively correlated with complete disappearance after TA; an MD > 25.0 mm indicated a reduced likelihood of complete disappearance compared with an MD ≤ 25.0 mm. An MD of 25.0 mm is an appropriate threshold of BTNs for TA on the basis of complete disappearance rate.

目的:热消融(TA)是治疗甲状腺良性结节(BTN)的一种安全有效的方法。然而,关于热消融治疗甲状腺良性结节的最佳最大直径(MD)尚未达成共识。本研究旨在根据TA术后完全消失率确定TA治疗BTN的最佳最大直径:这项回顾性研究纳入了 2014 年 6 月至 2022 年 1 月期间接受 TA 治疗的 639 例 BTN。总结了TA术后BTN的完全消失率,探讨了相关影响因素,并确定了TA术后BTN的最佳MD:最终随访(中位数:40 个月,范围:24-95 个月)时,总体体积缩小率为 95.4 ± 9.0%,50.5% 的 BTNs(323/639)完全消失。MD 与完全消失呈明显负相关(几率比 0.89,95% 置信区间 0.87-0.92;P 25.0 mm 的并发症发生率更高(6.5% 对 2.4%,P = 0.012):结论:BTN的MD与TA术后完全消失呈负相关;与MD≤25.0毫米相比,MD>25.0毫米表明完全消失的可能性降低。根据完全消失率,25.0 毫米的 MD 是 BTN 进行 TA 的合适阈值。
{"title":"Investigating the optimal maximum diameter of benign thyroid nodules for thermal ablation on the basis of complete disappearance rate.","authors":"Shi-Liang Cao, Wan-Ying Shi, Zhen-Long Zhao, Ying Wei, Na Yu, Jie Wu, Li-Li Peng, Yan Li, Ming-An Yu","doi":"10.1080/02656736.2024.2408374","DOIUrl":"https://doi.org/10.1080/02656736.2024.2408374","url":null,"abstract":"<p><strong>Objective: </strong>Thermal ablation (TA) is a safe and effective treatment for benign thyroid nodules (BTNs). However, there has been no consensus on the optimal maximum diameter (MD) of BTNs for TA. This study aimed to identify the optimal MD of BTNs for TA based on complete disappearance rate after TA.</p><p><strong>Materials and methods: </strong>This retrospective study included 639 BTNs treated with TA from June 2014 to January 2022. The complete disappearance rate of BTNs after TA was summarized, related influencing factors were explored, and the optimal MD of BTNs for TA was identified.</p><p><strong>Results: </strong>At the final follow-up (median: 40 months, range: 24-95 months), the overall volume reduction rate was 95.4 ± 9.0%, and 50.5% of the BTNs (323/639) completely disappeared. The MD was significantly negatively correlated with complete disappearance (odds ratio 0.89, 95% confidence interval 0.87-0.92; <i>p</i> < 0.001). Calcification, comet-tail artifacts, multilocular cysts, and composition of BTNs, as well as diabetes were negatively correlated with complete disappearance. Restricted cubic spline indicated that an MD of 25.0 mm was the optimal threshold of BTNs for TA, which was confirmed by subgroup logistic regression analysis. Compared with BTNs with MD ≤ 25.0 mm, those with MD > 25.0 mm had a greater complication rate (6.5% vs. 2.4%, <i>p</i> = 0.012).</p><p><strong>Conclusions: </strong>The MD of BTNs was negatively correlated with complete disappearance after TA; an MD > 25.0 mm indicated a reduced likelihood of complete disappearance compared with an MD ≤ 25.0 mm. An MD of 25.0 mm is an appropriate threshold of BTNs for TA on the basis of complete disappearance rate.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2408374"},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Time series analysis combined with transcriptome sequencing to explore characteristic genes and potential molecular mechanisms associated with ultrasound-guided microwave ablation of glioma. 时间序列分析与转录组测序相结合,探索与胶质瘤超声引导下微波消融相关的特征基因和潜在分子机制。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-09-24 DOI: 10.1080/02656736.2024.2406889
Qian Zhang, Guangfei Yang, Ruijiao Chang, Fuxia Wang, Tao Han, Jin Tian, Wen Wang

Objective: This study aimed to explore marker genes and their potential molecular mechanisms involved in US-guided MWA for glioma in mice.

Method: The differentially expressed genes (DEGs1 and DEGs2) and lncRNAs (DELs1 and DELs2) were obtained between Non (glioma tissues without MWA) and T0 groups (0h after MWA), as well as between Non and T24 groups (24h after MWA). The down-regulation cluster genes (CONDOWNDEGs) and upregulation cluster genes (CONUPDEGs) were identified by time series analysis. Candidate genes were obtained by overlapping CONDOWNDEGs with downregulation DEGs (DOWNDEGs)1 and DOWNDEGs2, as well as CONUPDEGs with up-regulation DEGs (UPDEGs)1 and UPDEGs2. The expressions of immune checkpoints and inflammatory factors, gene set enrichment analysis (GSEA), and protein subcellular localization were performed. The eXpression2Kinases (X2K), GeneMANIA, transcription factor (TF), and competing endogenous (ce) RNA regulatory networks were conducted. The expression of marker genes was validated by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: Five marker genes (IL32, VCAM1, IL34, NFKB1 and CXCL13) were identified, which were connected with immune-related functions. Two immune checkpoints (CD96 and TIGIT) and six inflammatory factors played key roles in US-guided MWA for glioma. ceRNA regulatory networks revealed that miR-625-5p, miR-625-3p, miR-31-5p and miR-671-5p were associated with target genes. qRT-PCR indicated both IL32, VCAM1, and NFKB1 were potential markers under US-guided MWA-related time series analysis.

Conclusion: The use of US-guided MWA might be a practical method for influencing the function of target genes, regulating time frames to decrease inflammation, and stimulating immune responses in glioma therapy.

研究目的本研究旨在探索小鼠脑胶质瘤US-guided MWA的标记基因及其潜在的分子机制:方法:研究小鼠神经胶质瘤组织中的差异表达基因(DEGs1和DEGs2)和lncRNAs(DELs1和DELs2)。通过时间序列分析确定了下调集群基因(CONDOWNDEGs)和上调集群基因(CONUPDEGs)。通过将 CONDOWNDEGs 与下调 DEGs(DOWNDEGs)1 和 DOWNDEGs2 重叠,以及 CONUPDEGs 与上调 DEGs(UPDEGs)1 和 UPDEGs2 重叠,得到候选基因。对免疫检查点和炎症因子的表达、基因组富集分析(GSEA)和蛋白质亚细胞定位进行了分析。还进行了eXpression2Kinases(X2K)、GeneMANIA、转录因子(TF)和竞争性内源性(ce)RNA调控网络的分析。通过实时定量聚合酶链反应(qRT-PCR)验证了标记基因的表达:结果:发现了五个标记基因(IL32、VCAM1、IL34、NFKB1 和 CXCL13)与免疫相关功能有关。ceRNA调控网络显示,miR-625-5p、miR-625-3p、miR-31-5p和miR-671-5p与靶基因相关;qRT-PCR显示,IL32、VCAM1和NFKB1是US-guided MWA相关时间序列分析的潜在标记物:结论:在胶质瘤治疗中,使用 US 引导的 MWA 可能是影响靶基因功能、调节时间框架以减少炎症和刺激免疫反应的一种实用方法。
{"title":"Time series analysis combined with transcriptome sequencing to explore characteristic genes and potential molecular mechanisms associated with ultrasound-guided microwave ablation of glioma.","authors":"Qian Zhang, Guangfei Yang, Ruijiao Chang, Fuxia Wang, Tao Han, Jin Tian, Wen Wang","doi":"10.1080/02656736.2024.2406889","DOIUrl":"https://doi.org/10.1080/02656736.2024.2406889","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore marker genes and their potential molecular mechanisms involved in US-guided MWA for glioma in mice.</p><p><strong>Method: </strong>The differentially expressed genes (DEGs1 and DEGs2) and lncRNAs (DELs1 and DELs2) were obtained between Non (glioma tissues without MWA) and T0 groups (0h after MWA), as well as between Non and T24 groups (24h after MWA). The down-regulation cluster genes (CONDOWNDEGs) and upregulation cluster genes (CONUPDEGs) were identified by time series analysis. Candidate genes were obtained by overlapping CONDOWNDEGs with downregulation DEGs (DOWNDEGs)1 and DOWNDEGs2, as well as CONUPDEGs with up-regulation DEGs (UPDEGs)1 and UPDEGs2. The expressions of immune checkpoints and inflammatory factors, gene set enrichment analysis (GSEA), and protein subcellular localization were performed. The eXpression2Kinases (X2K), GeneMANIA, transcription factor (TF), and competing endogenous (ce) RNA regulatory networks were conducted. The expression of marker genes was validated by quantitative real-time polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>Five marker genes (<i>IL32, VCAM1</i>, <i>IL34</i>, <i>NFKB1</i> and <i>CXCL13</i>) were identified, which were connected with immune-related functions. Two immune checkpoints (CD96 and TIGIT) and six inflammatory factors played key roles in US-guided MWA for glioma. ceRNA regulatory networks revealed that miR-625-5p, miR-625-3p, miR-31-5p and miR-671-5p were associated with target genes. qRT-PCR indicated both <i>IL32</i>, <i>VCAM1</i>, and <i>NFKB1</i> were potential markers under US-guided MWA-related time series analysis.</p><p><strong>Conclusion: </strong>The use of US-guided MWA might be a practical method for influencing the function of target genes, regulating time frames to decrease inflammation, and stimulating immune responses in glioma therapy.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2406889"},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Risk assessment and prediction of occult uterine sarcoma in patients with presumed uterine fibroids before high-intensity focused ultrasound treatment. 高强度聚焦超声治疗前对子宫肌瘤假定患者隐匿性子宫肉瘤的风险评估和预测。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-07-31 DOI: 10.1080/02656736.2024.2385600
Qian Wang, Zhenjiang Lin, Xiaogang Zhu, Yangyang Wang, Ying Zhang, Min He, Lian Zhang

Objective: To develop a diagnostic model for predicting occult uterine sarcoma in patients with presumed uterine fibroids.

Materials and methods: We retrospectively reviewed 41631 patients with presumed uterine fibroids who presented for HIFU treatment in 13 hospitals between November 2008 and October 2023. Of these patients, 27 with occult uterine sarcoma and 54 with uterine fibroids were enrolled. Univariate analysis and multivariate logistics regression analysis were used to determine the independent risk factors for the diagnosis of occult uterine sarcoma. A prediction model was constructed based on the coefficients of the risk factors.

Results: The multivariate analysis revealed abnormal vaginal bleeding, ill-defined boundary of tumor, hyperintensity on T2WI, and central unenhanced areas as independent risk factors. A scoring system was created to assess for occult uterine sarcoma risk. The score for abnormal vaginal bleeding was 56. The score for ill-defined lesion boundary was 90. The scores for lesions with hypointensity, isointensity signal/heterogeneous signal intensity, and hyperintensity on T2WI were 0, 42, and 93, respectively. The scores for lesions without enhancement on the mass margin, uniform enhancement of tumor, and no enhancement in the center of tumor were 0, 20, and 100, respectively. Patients with a higher total score implied a higher likelihood of a diagnosis of occult uterine sarcoma than that of patients with a lower score. The established model showed good predictive efficacy.

Conclusions: Our results demonstrated that the diagnostic prediction model can be used to evaluate the risk of uterine sarcoma in patients with presumed uterine fibroids.

目的:建立一个诊断模型,用于预测子宫肌瘤患者的隐匿性子宫肉瘤:建立一个诊断模型,用于预测假定子宫肌瘤患者的隐匿性子宫肉瘤:我们回顾性研究了 2008 年 11 月至 2023 年 10 月期间在 13 家医院接受 HIFU 治疗的 41631 例子宫肌瘤患者。其中,27 例为隐匿性子宫肉瘤患者,54 例为子宫肌瘤患者。采用单变量分析和多变量物流回归分析来确定隐匿性子宫肉瘤诊断的独立风险因素。根据风险因素的系数建立了预测模型:多变量分析显示,异常阴道出血、肿瘤边界不清晰、T2WI高密度和中心未增强区是独立的风险因素。我们建立了一个评分系统来评估隐匿性子宫肉瘤的风险。异常阴道出血的评分为 56 分。病灶边界不清的评分为 90 分。T2WI 低密度、等密度信号/异质信号强度和高密度病变的得分分别为 0、42 和 93 分。肿块边缘无强化、肿瘤均匀强化和肿瘤中心无强化的病灶得分分别为 0、20 和 100。总分较高的患者被诊断为隐匿性子宫肉瘤的可能性高于总分较低的患者。所建立的模型具有良好的预测效果:我们的研究结果表明,诊断预测模型可用于评估子宫肌瘤患者罹患子宫肉瘤的风险。
{"title":"Risk assessment and prediction of occult uterine sarcoma in patients with presumed uterine fibroids before high-intensity focused ultrasound treatment.","authors":"Qian Wang, Zhenjiang Lin, Xiaogang Zhu, Yangyang Wang, Ying Zhang, Min He, Lian Zhang","doi":"10.1080/02656736.2024.2385600","DOIUrl":"https://doi.org/10.1080/02656736.2024.2385600","url":null,"abstract":"<p><strong>Objective: </strong>To develop a diagnostic model for predicting occult uterine sarcoma in patients with presumed uterine fibroids.</p><p><strong>Materials and methods: </strong>We retrospectively reviewed 41631 patients with presumed uterine fibroids who presented for HIFU treatment in 13 hospitals between November 2008 and October 2023. Of these patients, 27 with occult uterine sarcoma and 54 with uterine fibroids were enrolled. Univariate analysis and multivariate logistics regression analysis were used to determine the independent risk factors for the diagnosis of occult uterine sarcoma. A prediction model was constructed based on the coefficients of the risk factors.</p><p><strong>Results: </strong>The multivariate analysis revealed abnormal vaginal bleeding, ill-defined boundary of tumor, hyperintensity on T2WI, and central unenhanced areas as independent risk factors. A scoring system was created to assess for occult uterine sarcoma risk. The score for abnormal vaginal bleeding was 56. The score for ill-defined lesion boundary was 90. The scores for lesions with hypointensity, isointensity signal/heterogeneous signal intensity, and hyperintensity on T2WI were 0, 42, and 93, respectively. The scores for lesions without enhancement on the mass margin, uniform enhancement of tumor, and no enhancement in the center of tumor were 0, 20, and 100, respectively. Patients with a higher total score implied a higher likelihood of a diagnosis of occult uterine sarcoma than that of patients with a lower score. The established model showed good predictive efficacy.</p><p><strong>Conclusions: </strong>Our results demonstrated that the diagnostic prediction model can be used to evaluate the risk of uterine sarcoma in patients with presumed uterine fibroids.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2385600"},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regional hyperthermia for soft tissue sarcoma - a survey on current practice, controversies and consensus among 12 European centers. 软组织肉瘤区域热疗--12 个欧洲中心对当前做法、争议和共识的调查。
IF 3.1 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-04-23 DOI: 10.1080/02656736.2024.2342348
Siyer Roohani, Felix Ehret, Marcus Beck, Danai P Veltsista, Jacek Nadobny, Sebastian Zschaeck, Sultan Abdel-Rahman, Franziska Eckert, Anne Flörcken, Rolf D Issels, Stephan Klöck, Robert Krempien, Lars H Lindner, Markus Notter, Oliver J Ott, Daniel Pink, Vlatko Potkrajcic, Peter Reichardt, Oliver Riesterer, Mateusz Jacek Spałek, Emanuel Stutz, Rüdiger Wessalowski, Thomas Zilli, Daniel Zips, Pirus Ghadjar, David Kaul

Purpose: To analyze the current practice of regional hyperthermia (RHT) for soft tissue sarcoma (STS) at 12 European centers to provide an overview, find consensuses and identify controversies necessary for future guidelines and clinical trials.

Methods: In this cross-sectional survey study, a 27-item questionnaire assessing clinical subjects and procedural details on RHT for STS was distributed to 12 European cancer centers for RHT.

Results: We have identified seven controversies and five consensus points. Of 12 centers, 6 offer both, RHT with chemotherapy (CTX) or with radiotherapy (RT). Two centers only offer RHT with CTX and four centers only offer RHT with RT. All 12 centers apply RHT for localized, high-risk STS of the extremities, trunk wall and retroperitoneum. However, eight centers also use RHT in metastatic STS, five in palliative STS, eight for superficial STS and six for low-grade STS. Pretherapeutic imaging for RHT treatment planning is used by 10 centers, 9 centers set 40-43 °C as the intratumoral target temperature, and all centers use skin detectors or probes in body orifices for thermometry.

Discussion: There is disagreement regarding the integration of RHT in contemporary interdisciplinary care of STS patients. Many clinical controversies exist that require a standardized consensus guideline and innovative study ideas. At the same time, our data has shown that existing guidelines and decades of experience with the technique of RHT have mostly standardized procedural aspects.

Conclusions: The provided results may serve as a basis for future guidelines and inform future clinical trials for RHT in STS patients.

目的:分析12个欧洲中心目前对软组织肉瘤(STS)进行区域热疗(RHT)的实践情况,为未来的指南和临床试验提供一个概览,找到共识并找出必要的争议点:在这项横断面调查研究中,我们向 12 个欧洲癌症中心的 RHT 患者发放了一份 27 个项目的调查问卷,评估 STS RHT 的临床对象和手术细节:结果:我们发现了七个争议点和五个共识点。在 12 个中心中,有 6 个中心同时提供 RHT 和化疗 (CTX) 或放疗 (RT)。有两家中心只提供结合 CTX 的 RHT,有四家中心只提供结合 RT 的 RHT。所有 12 个中心都将 RHT 用于四肢、躯干壁和腹膜后的局部高危 STS。不过,也有 8 个中心将 RHT 用于转移性 STS,5 个中心用于姑息性 STS,8 个中心用于浅表性 STS,6 个中心用于低级别 STS。10个中心使用治疗前成像来制定RHT治疗计划,9个中心将40-43 °C作为瘤内目标温度,所有中心都使用皮肤探测器或体腔探头进行测温:讨论:对于将 RHT 纳入 STS 患者的跨学科治疗,目前还存在分歧。许多临床争议需要标准化的共识指南和创新的研究理念。与此同时,我们的数据显示,现有的指南和数十年的 RHT 技术经验已基本实现了程序方面的标准化:所提供的结果可作为未来指南的依据,并为未来 STS 患者 RHT 的临床试验提供参考。
{"title":"Regional hyperthermia for soft tissue sarcoma - a survey on current practice, controversies and consensus among 12 European centers.","authors":"Siyer Roohani, Felix Ehret, Marcus Beck, Danai P Veltsista, Jacek Nadobny, Sebastian Zschaeck, Sultan Abdel-Rahman, Franziska Eckert, Anne Flörcken, Rolf D Issels, Stephan Klöck, Robert Krempien, Lars H Lindner, Markus Notter, Oliver J Ott, Daniel Pink, Vlatko Potkrajcic, Peter Reichardt, Oliver Riesterer, Mateusz Jacek Spałek, Emanuel Stutz, Rüdiger Wessalowski, Thomas Zilli, Daniel Zips, Pirus Ghadjar, David Kaul","doi":"10.1080/02656736.2024.2342348","DOIUrl":"10.1080/02656736.2024.2342348","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze the current practice of regional hyperthermia (RHT) for soft tissue sarcoma (STS) at 12 European centers to provide an overview, find consensuses and identify controversies necessary for future guidelines and clinical trials.</p><p><strong>Methods: </strong>In this cross-sectional survey study, a 27-item questionnaire assessing clinical subjects and procedural details on RHT for STS was distributed to 12 European cancer centers for RHT.</p><p><strong>Results: </strong>We have identified seven controversies and five consensus points. Of 12 centers, 6 offer both, RHT with chemotherapy (CTX) or with radiotherapy (RT). Two centers only offer RHT with CTX and four centers only offer RHT with RT. All 12 centers apply RHT for localized, high-risk STS of the extremities, trunk wall and retroperitoneum. However, eight centers also use RHT in metastatic STS, five in palliative STS, eight for superficial STS and six for low-grade STS. Pretherapeutic imaging for RHT treatment planning is used by 10 centers, 9 centers set 40-43 °C as the intratumoral target temperature, and all centers use skin detectors or probes in body orifices for thermometry.</p><p><strong>Discussion: </strong>There is disagreement regarding the integration of RHT in contemporary interdisciplinary care of STS patients. Many clinical controversies exist that require a standardized consensus guideline and innovative study ideas. At the same time, our data has shown that existing guidelines and decades of experience with the technique of RHT have mostly standardized procedural aspects.</p><p><strong>Conclusions: </strong>The provided results may serve as a basis for future guidelines and inform future clinical trials for RHT in STS patients.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2342348"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of factors affecting pregnancy outcomes in patients with adenomyosis after high intensity focused ultrasound ablation: a retrospective study. 高强度聚焦超声消融术后影响子宫腺肌症患者妊娠结局的因素分析:一项回顾性研究。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-08-19 DOI: 10.1080/02656736.2024.2390124
Rong Ma, Diego Armando Burgos Briones, Min Zou, Yu Xiong, Jin Bai, Lian Zhang

Objectives: To investigate all pregnancies and analyze the factors influencing pregnancy outcomes in patients with adenomyosis after high intensity focused ultrasound (HIFU).

Materials and methods: A total of 231 patients with adenomyosis who completed HIFU and wished to conceive were enrolled. The symptom improvement and information of pregnancy were recorded during the follow-up period. Factors influencing pregnancy outcomes were analyzed using multivariate regression analysis and survival analysis.

Results: After HIFU, 100 of 231 (43.3%) patients became pregnant within 96 months, including 77 (77/194, 39.7%) in natural and 23 (23/37, 62.2%) in vitro fertilization and embryo transfer (IVF-ET) pregnancies following gonadotropin-releasing hormone agonist (GnRHa). Among the 108 (46.8%, 108/231) infertile patients (defined as the failure to achieve pregnancy after 12 months of regular unprotected sexual intercourse, 40 primary infertility and 68 secondary infertility), 31 (28.7%) became pregnant. At the end of the follow-up, 70 successfully delivered 71 healthy babies. No uterine rupture occurred during pregnancy and delivery. Patients with pelvic adhesion and infertility history had a lower pregnancy chance than that of patients without pelvic adhesion and infertility history (OR < 1, p < 0.05). Patients with small adenomyotic lesion volume had a greater pregnancy chance than that of patients with large lesion volume (OR < 1, p < 0.05). IVF-ET following GnRHa had a better pregnancy chance (p < 0.05).

Conclusions: HIFU seems to have a beneficial effect on fertility of patients with adenomyosis. Pelvic adhesion, infertility history, and large adenomyotic lesion volume have adverse effects on pregnancy, but IVF-ET following GnRHa after HIFU could increase the pregnancy chance.

摘要调查腺肌症患者在接受高强度聚焦超声(HIFU)治疗后的所有妊娠情况,并分析影响妊娠结局的因素:共纳入 231 例完成 HIFU 并希望怀孕的子宫腺肌症患者。随访期间记录了症状改善情况和怀孕信息。采用多变量回归分析和生存分析对影响妊娠结局的因素进行了分析:HIFU术后,231名患者中有100人(43.3%)在96个月内怀孕,其中77人(77/194,39.7%)自然怀孕,23人(23/37,62.2%)使用促性腺激素释放激素激动剂(GnRHa)后进行体外受精和胚胎移植(IVF-ET)怀孕。在 108 名(46.8%,108/231)不孕症患者(定义为定期无保护性交 12 个月后仍未怀孕,40 名原发性不孕症患者和 68 名继发性不孕症患者)中,31 名(28.7%)患者怀孕。随访结束时,70 名患者成功分娩了 71 名健康婴儿。在怀孕和分娩期间,没有发生子宫破裂。与无盆腔粘连和不孕史的患者相比,有盆腔粘连和不孕史的患者怀孕几率较低(OR < 1,p p p 结论:HIFU 似乎对不孕症患者有益:HIFU 似乎对子宫腺肌症患者的生育有好处。盆腔粘连、不孕史、腺肌症病灶体积大对妊娠有不利影响,但HIFU术后GnRHa后的IVF-ET可增加妊娠几率。
{"title":"Analysis of factors affecting pregnancy outcomes in patients with adenomyosis after high intensity focused ultrasound ablation: a retrospective study.","authors":"Rong Ma, Diego Armando Burgos Briones, Min Zou, Yu Xiong, Jin Bai, Lian Zhang","doi":"10.1080/02656736.2024.2390124","DOIUrl":"https://doi.org/10.1080/02656736.2024.2390124","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate all pregnancies and analyze the factors influencing pregnancy outcomes in patients with adenomyosis after high intensity focused ultrasound (HIFU).</p><p><strong>Materials and methods: </strong>A total of 231 patients with adenomyosis who completed HIFU and wished to conceive were enrolled. The symptom improvement and information of pregnancy were recorded during the follow-up period. Factors influencing pregnancy outcomes were analyzed using multivariate regression analysis and survival analysis.</p><p><strong>Results: </strong>After HIFU, 100 of 231 (43.3%) patients became pregnant within 96 months, including 77 (77/194, 39.7%) in natural and 23 (23/37, 62.2%) <i>in vitro</i> fertilization and embryo transfer (IVF-ET) pregnancies following gonadotropin-releasing hormone agonist (GnRHa). Among the 108 (46.8%, 108/231) infertile patients (defined as the failure to achieve pregnancy after 12 months of regular unprotected sexual intercourse, 40 primary infertility and 68 secondary infertility), 31 (28.7%) became pregnant. At the end of the follow-up, 70 successfully delivered 71 healthy babies. No uterine rupture occurred during pregnancy and delivery. Patients with pelvic adhesion and infertility history had a lower pregnancy chance than that of patients without pelvic adhesion and infertility history (OR < 1, <i>p</i> < 0.05). Patients with small adenomyotic lesion volume had a greater pregnancy chance than that of patients with large lesion volume (OR < 1, <i>p</i> < 0.05). IVF-ET following GnRHa had a better pregnancy chance (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>HIFU seems to have a beneficial effect on fertility of patients with adenomyosis. Pelvic adhesion, infertility history, and large adenomyotic lesion volume have adverse effects on pregnancy, but IVF-ET following GnRHa after HIFU could increase the pregnancy chance.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2390124"},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vivo electrical properties of the healthy liver and the hepatic tumor in a mouse model between 1 Hz and 1 MHz during a thermal treatment. 在热处理过程中,健康肝脏和肝肿瘤小鼠模型在 1 赫兹和 1 兆赫之间的体内电特性。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-09-01 DOI: 10.1080/02656736.2024.2396122
Yitong Guo, Weice Wang, Weichen Li, Junyao Li, Mingxu Zhu, Ruteng Song, Wenjing Zhu, Lei Wang, Zhenyu Ji, Xuetao Shi

Objective: Understansding the changing patterns of in vivo electrical properties for the target tissue is crucial for the accurate temperature monitoring and the treatment efficacy in thermal therapy. Our research aims to investigate the changing patterns and the reversibility of in vivo electrical properties for both healthy livers and liver tumors in a mouse model over a frequency range of 1 Hz to 1 MHz at temperatures between 30 °C to 90 °C.

Methods and materials: The mice were anesthetized and the target organ was exposed. An 808-nm near-infrared laser was employed as the heating source to heat the organ in vivo. The four-needle electrode, connected to an impedance analyzer, was utilized to obtain the impedance at varying temperatures, which were monitored by a thermocouple.

Results: The findings indicated a gradual decline in impedance with an increase in temperature. Furthermore, the impedance was normalized to that at 30 °C, and the real part of the normalized impedance was defined as the k-values, which range from 0 to 1. The results demonstrated a linear correlation between k-values and temperatures (R2 > 0.9 for livers and R2 > 0.8 for tumors). Significant differences were observed between livers and tumors at 1, 10 and 50 kHz (p < 0.05). Additionally, it was demonstrated that the electrical properties could be reversed when the temperature was below or equal to 45 °C.

Conclusion: We believe that these results will contribute to the advancement of radiofrequency ablation systems and the development of techniques for temperature monitoring during liver thermal treatment.

目的:了解目标组织体内电特性的变化规律对于准确监测温度和热疗疗效至关重要。我们的研究旨在研究小鼠模型中健康肝脏和肝脏肿瘤在 30 °C 至 90 °C 温度下,在 1 Hz 至 1 MHz 频率范围内体内电特性的变化规律和可逆性:麻醉小鼠并暴露靶器官。采用波长为 808 纳米的近红外激光作为加热源,对体内器官进行加热。利用连接阻抗分析仪的四针电极获得不同温度下的阻抗,并通过热电偶进行监测:结果:研究结果表明,随着温度的升高,阻抗逐渐下降。此外,阻抗被归一化为 30 °C 时的阻抗,归一化阻抗的实部被定义为 k 值,范围在 0 至 1 之间。结果表明,k 值与温度呈线性相关(肝脏的 R2 > 0.9,肿瘤的 R2 > 0.8)。在 1、10 和 50 kHz 频率下,肝脏和肿瘤之间存在显著差异(p):我们相信,这些结果将有助于射频消融系统的进步和肝脏热处理期间温度监测技术的发展。
{"title":"<i>In vivo</i> electrical properties of the healthy liver and the hepatic tumor in a mouse model between 1 Hz and 1 MHz during a thermal treatment.","authors":"Yitong Guo, Weice Wang, Weichen Li, Junyao Li, Mingxu Zhu, Ruteng Song, Wenjing Zhu, Lei Wang, Zhenyu Ji, Xuetao Shi","doi":"10.1080/02656736.2024.2396122","DOIUrl":"https://doi.org/10.1080/02656736.2024.2396122","url":null,"abstract":"<p><p><b>Objective:</b> Understansding the changing patterns of <i>in vivo</i> electrical properties for the target tissue is crucial for the accurate temperature monitoring and the treatment efficacy in thermal therapy. Our research aims to investigate the changing patterns and the reversibility of <i>in vivo</i> electrical properties for both healthy livers and liver tumors in a mouse model over a frequency range of 1 Hz to 1 MHz at temperatures between 30 °C to 90 °C.</p><p><p><b>Methods and materials:</b> The mice were anesthetized and the target organ was exposed. An 808-nm near-infrared laser was employed as the heating source to heat the organ <i>in vivo</i>. The four-needle electrode, connected to an impedance analyzer, was utilized to obtain the impedance at varying temperatures, which were monitored by a thermocouple.</p><p><p><b>Results:</b> The findings indicated a gradual decline in impedance with an increase in temperature. Furthermore, the impedance was normalized to that at 30 °C, and the real part of the normalized impedance was defined as the k-values, which range from 0 to 1. The results demonstrated a linear correlation between k-values and temperatures (R<sup>2</sup> > 0.9 for livers and R<sup>2</sup> > 0.8 for tumors). Significant differences were observed between livers and tumors at 1, 10 and 50 kHz (<i>p</i> < 0.05). Additionally, it was demonstrated that the electrical properties could be reversed when the temperature was below or equal to 45 °C.</p><p><p><b>Conclusion:</b> We believe that these results will contribute to the advancement of radiofrequency ablation systems and the development of techniques for temperature monitoring during liver thermal treatment.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2396122"},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryoablation combined with dual immune checkpoint blockade enhances antitumor efficacy in hepatocellular carcinoma model mice. 冷冻消融联合双重免疫检查点阻断疗法可提高肝细胞癌模型小鼠的抗肿瘤疗效。
IF 3 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-07-02 DOI: 10.1080/02656736.2024.2373319
Jun Gu, Zepeng Yu, Xiangxiang Tang, Wenying Chen, Xuedong Deng, Xiaoli Zhu

Background: Cryoablation (Cryo) is a minimally invasive treatment for tumors. Cryo can activate the body's immune response, although it is typically weak. The immune response induced by Cryo in hepatocellular carcinoma (HCC) is poorly understood. PD-1 and CTLA-4 monoclonal antibodies are immune checkpoint inhibitors used in immunotherapy for tumors. The combined use of these antibodies with Cryo may enhance the immune effect.

Methods: A Balb/c mouse model of HCC was established and treated with Cryo, immune checkpoint blockade (ICB), or Cryo + ICB (combination therapy). The growth trend of right untreated tumors and survival time of mice were determined. The expression of apoptosis-related proteins was detected by Western blot (WB) assay. The percentages of immune cells and immunosuppressive cells were analyzed by flow cytometry. The numbers of infiltrating T lymphocytes were checked by immunohistochemistry, and the levels of T-cell-associated cytokines were detected by Quantitative real-time Polymerase Chain Reaction (qRT-PCR) assays and Enzyme-Linked Immunosorbent Assays (ELISA) assays.

Results: Cryo + ICB inhibited the growth of right untreated tumors, promoted tumor cell apoptosis, and prolonged the survival time of mice. Local T-cell infiltration in right tumor tissues increased after the combination therapy, while the number of immunosuppressive cells was significantly reduced. In addition, the combination therapy may induce the production of multiple Th1-type cytokines but reduce the production of Th2-type cytokines.

Conclusions: Cryo can activate CD8+ and CD4+ T-cell immune responses. Cryo + ICB can relieve the immunosuppressive tumor microenvironment and shift the Th1/Th2 balance toward Th1 dominance, further enhancing the Cryo-induced T-cell immune response and resulting in a stronger antitumor immune response.

背景:低温消融术(Cryo)是一种微创的肿瘤治疗方法。冷冻可激活机体的免疫反应,但通常较弱。冷冻疗法在肝细胞癌(HCC)中诱导的免疫反应尚不清楚。PD-1 和 CTLA-4 单克隆抗体是用于肿瘤免疫疗法的免疫检查点抑制剂。将这些抗体与冷冻联合使用可能会增强免疫效果:方法:建立 Balb/c 小鼠 HCC 模型,并使用 Cryo、免疫检查点阻断疗法(ICB)或 Cryo + ICB(联合疗法)进行治疗。测定未经治疗的右侧肿瘤的生长趋势和小鼠的存活时间。通过 Western 印迹(WB)检测凋亡相关蛋白的表达。流式细胞术分析了免疫细胞和免疫抑制细胞的百分比。免疫组化法检测浸润的 T 淋巴细胞数量,定量实时聚合酶链式反应(qRT-PCR)和酶联免疫吸附试验(ELISA)检测 T 细胞相关细胞因子的水平:低温+ ICB抑制了未经治疗的右侧肿瘤的生长,促进了肿瘤细胞的凋亡,延长了小鼠的存活时间。联合治疗后,右侧肿瘤组织中的局部 T 细胞浸润增加,而免疫抑制细胞的数量明显减少。此外,联合疗法可诱导产生多种Th1型细胞因子,但减少了Th2型细胞因子的产生:结论:低温疗法可激活 CD8+ 和 CD4+ T 细胞免疫反应。低温+ ICB 可以缓解肿瘤微环境的免疫抑制作用,并使 Th1/Th2 平衡向 Th1 主导方向转变,进一步增强低温诱导的 T 细胞免疫反应,从而产生更强的抗肿瘤免疫反应。
{"title":"Cryoablation combined with dual immune checkpoint blockade enhances antitumor efficacy in hepatocellular carcinoma model mice.","authors":"Jun Gu, Zepeng Yu, Xiangxiang Tang, Wenying Chen, Xuedong Deng, Xiaoli Zhu","doi":"10.1080/02656736.2024.2373319","DOIUrl":"https://doi.org/10.1080/02656736.2024.2373319","url":null,"abstract":"<p><strong>Background: </strong>Cryoablation (Cryo) is a minimally invasive treatment for tumors. Cryo can activate the body's immune response, although it is typically weak. The immune response induced by Cryo in hepatocellular carcinoma (HCC) is poorly understood. PD-1 and CTLA-4 monoclonal antibodies are immune checkpoint inhibitors used in immunotherapy for tumors. The combined use of these antibodies with Cryo may enhance the immune effect.</p><p><strong>Methods: </strong>A Balb/c mouse model of HCC was established and treated with Cryo, immune checkpoint blockade (ICB), or Cryo + ICB (combination therapy). The growth trend of right untreated tumors and survival time of mice were determined. The expression of apoptosis-related proteins was detected by Western blot (WB) assay. The percentages of immune cells and immunosuppressive cells were analyzed by flow cytometry. The numbers of infiltrating T lymphocytes were checked by immunohistochemistry, and the levels of T-cell-associated cytokines were detected by Quantitative real-time Polymerase Chain Reaction (qRT-PCR) assays and Enzyme-Linked Immunosorbent Assays (ELISA) assays.</p><p><strong>Results: </strong>Cryo + ICB inhibited the growth of right untreated tumors, promoted tumor cell apoptosis, and prolonged the survival time of mice. Local T-cell infiltration in right tumor tissues increased after the combination therapy, while the number of immunosuppressive cells was significantly reduced. In addition, the combination therapy may induce the production of multiple Th1-type cytokines but reduce the production of Th2-type cytokines.</p><p><strong>Conclusions: </strong>Cryo can activate CD8<sup>+</sup> and CD4<sup>+</sup> T-cell immune responses. Cryo + ICB can relieve the immunosuppressive tumor microenvironment and shift the Th1/Th2 balance toward Th1 dominance, further enhancing the Cryo-induced T-cell immune response and resulting in a stronger antitumor immune response.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2373319"},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Magnetic hybrid Pd/Fe-oxide nanoparticles meet the demands for ablative thermo-brachytherapy. 磁性钯/铁-氧化物混合纳米粒子可满足烧蚀热近距离治疗的需求。
IF 3.1 3区 医学 Q2 ONCOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-08 DOI: 10.1080/02656736.2023.2299480
Rogier van Oossanen, Alexandra Maier, Jérémy Godart, Jean-Philippe Pignol, Antonia G Denkova, Gerard C van Rhoon, Kristina Djanashvili

Objective: To investigate the potential of hybrid Pd/Fe-oxide magnetic nanoparticles designed for thermo-brachytherapy of breast cancer, considering their specific loss power (SLP) and clinical constraints in the applied magnetic field.

Methods: Hybrid nanoparticles consisting of palladium-core and iron oxide shell of increasing thickness, were suspended in water and their SLPs were measured at varying magnetic fields (12-26 mT peak) and frequencies (50-730 kHz) with a commercial alternating magnetic field generator (magneTherm™ Digital, nanoTherics Ltd.).

Results: Validation of the heating device used in this study with commercial HyperMag-C nanoparticles showed a small deviation (±4%) over a period of 1 year, confirming the reliability of the method. The integration of dual thermometers, one in the center and one at the bottom of the sample vial, allowed monitoring of homogeneity of the sample suspensions. SLPs measurements on a series of nanoparticles of increasing sizes showed the highest heating for the diameter of 21 nm (SLP = 225 W/g) at the applied frequencies of 346 and 730 kHz. No heating was observed for the nanoparticles with the size <14 nm, confirming the importance of the size-parameter. The heating ability of the best performing Pd/Fe-oxide-21 was calculated to be sufficient to ablate tumors with a radius ±4 and 12 mm using 10 and 1 mg/mL nanoparticle concentration, respectively.

Conclusions: Nanoparticles consisting of non-magnetic palladium-core and magnetic iron oxide shell are suitable for magnetic hyperthermia/thermal ablation under clinically safe conditions of 346 kHz and 19.1 mT, with minimal eddy current effects in combination with maximum SLP.

目的研究设计用于乳腺癌热近距离治疗的钯/氧化铁混合磁性纳米粒子的潜力,同时考虑其在应用磁场中的比损耗功率(SLP)和临床限制因素。方法:将由钯核和厚度不断增加的氧化铁壳组成的混合纳米粒子悬浮在水中,使用商用交变磁场发生器(magneTherm™ Digital,nanoTherics Ltd.)在不同磁场(12-26 mT 峰值)和频率(50-730 kHz)下测量它们的比损耗功率:使用商用 HyperMag-C 纳米粒子对本研究中使用的加热装置进行了验证,结果表明,在一年的时间内,偏差很小(±4%),这证实了该方法的可靠性。双温度计(一个位于样品瓶中心,另一个位于底部)的集成可监测样品悬浮液的均匀性。对一系列尺寸不断增大的纳米颗粒进行的 SLPs 测量显示,在 346 和 730 kHz 的应用频率下,直径为 21 nm(SLP = 225 W/g)的纳米颗粒发热量最高。尺寸结论中的纳米颗粒未观察到加热现象:由非磁性钯核和磁性氧化铁外壳组成的纳米粒子适用于在 346 kHz 和 19.1 mT 的临床安全条件下进行磁热疗/热消融,结合最大 SLP,涡流效应最小。
{"title":"Magnetic hybrid Pd/Fe-oxide nanoparticles meet the demands for ablative thermo-brachytherapy.","authors":"Rogier van Oossanen, Alexandra Maier, Jérémy Godart, Jean-Philippe Pignol, Antonia G Denkova, Gerard C van Rhoon, Kristina Djanashvili","doi":"10.1080/02656736.2023.2299480","DOIUrl":"10.1080/02656736.2023.2299480","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the potential of hybrid Pd/Fe-oxide magnetic nanoparticles designed for thermo-brachytherapy of breast cancer, considering their specific loss power (<i>SLP</i>) and clinical constraints in the applied magnetic field.</p><p><strong>Methods: </strong>Hybrid nanoparticles consisting of palladium-core and iron oxide shell of increasing thickness, were suspended in water and their <i>SLPs</i> were measured at varying magnetic fields (12-26 mT peak) and frequencies (50-730 kHz) with a commercial alternating magnetic field generator (magneTherm™ Digital, nanoTherics Ltd.).</p><p><strong>Results: </strong>Validation of the heating device used in this study with commercial HyperMag-C nanoparticles showed a small deviation (±4%) over a period of 1 year, confirming the reliability of the method. The integration of dual thermometers, one in the center and one at the bottom of the sample vial, allowed monitoring of homogeneity of the sample suspensions. <i>SLP</i>s measurements on a series of nanoparticles of increasing sizes showed the highest heating for the diameter of 21 nm (<i>SLP</i> = 225 W/g) at the applied frequencies of 346 and 730 kHz. No heating was observed for the nanoparticles with the size <14 nm, confirming the importance of the size-parameter. The heating ability of the best performing Pd/Fe-oxide-21 was calculated to be sufficient to ablate tumors with a radius ±4 and 12 mm using 10 and 1 mg/mL nanoparticle concentration, respectively.</p><p><strong>Conclusions: </strong>Nanoparticles consisting of non-magnetic palladium-core and magnetic iron oxide shell are suitable for magnetic hyperthermia/thermal ablation under clinically safe conditions of 346 kHz and 19.1 mT, with minimal eddy current effects in combination with maximum <i>SLP</i>.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":"41 1","pages":"2299480"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International Journal of Hyperthermia
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1