Pub Date : 2018-07-01DOI: 10.18052/WWW.SCIPRESS.COM/IJPPE.10.29
S. Kaushik, M. Asif
Solanesol is the starting material for many high value biochemicals, including Co-enzyme Q10 and vitamin-K analogues. The aim of the current study was to develop and validate a reliable and fast analytical procedure for the determination of solanesol in Nicotianatabacum using high-performance thin layer chromatography (HPTLC) method. The method was developed on TLC aluminium plates precoated with silica gel 60F-254 using solvent system hexane: ethyl acetate (5:1, v/v), which gives compact spot of solanesol (Rf value 0.41 ± 0.02). Densitometric analysis of solanesol was carried out in the absorbance mode at 210 nm. The linear regression analysis data for the calibration plot showed good linear relationship with r = 0.9978 with respect to peak area, in the concentration rang 100-5000 ng per spot of solanesol. The limit of detection and quantification were 13 and 30 ng per spot, respectively. The proposed method was applied for quantitative estimation of solanesol in different parts of Nicotianatabacum from different geographical regions in India, which showed that maximum amount of solanesol was found to be present in leaf sample collected from Karnataka i.e. 3.52 mg/g. Statistical analysis proved that the method is repeatable, selective and accurate for the estimation of solanesol in Nicotianatabacum.
茄尼醇是许多高价值生物化学物质的原料，包括辅酶Q10和维生素k类似物。本研究旨在建立高效薄层色谱法测定烟叶中茄尼醇的快速、可靠的分析方法。采用溶剂体系己烷:乙酸乙酯(5:1,v/v)，在硅胶60F-254预涂的薄层铝板上建立了该方法，得到了紧凑的茄尼醇斑点(Rf值0.41±0.02)。在210 nm吸光度模式下对茄尼醇进行密度分析。在茄尼醇浓度100 ~ 5000 ng /点范围内，校正图的线性回归分析数据与峰面积呈良好的线性关系，r = 0.9978。检测限为13 ng /点，定量限为30 ng /点。将该方法应用于印度不同地理区域烟叶中茄尼醇含量的定量测定，结果表明，在印度卡纳塔克邦采集的烟叶样品中茄尼醇含量最高，为3.52 mg/g。统计分析表明，该方法重复性好、选择性好、准确度高，可用于烟叶中茄尼醇的含量测定。
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