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Detection and measurement of radioactive substances in water and food: a narrative review 水和食物中放射性物质的检测和测量:叙述性综述
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-26 DOI: 10.4081/ijfs.2024.11651
Fateme Asadi Touranlou, Minoo Moghimani, M. Marhamati, Mitra Rezaei
Contamination of food and water with radioactive substances is a serious health problem. There are several methods to detect and measure radioactive materials, some of which have been developed in recent years. This paper aims to discuss the methods of detecting and measuring radioactive substances in food and water. The principles and the advantages and disadvantages of each method have been discussed. The results showed that some of these methods, such as spectrometry γ-ray high purity germanium, portable radon gas surveyor SILENA, RAD7, and inductively coupled plasma mass spectrometry, have a higher sensitivity for detection and measurement. Also, the spectrometry γ-ray high purity germanium method has attracted more attention than other methods because it can measure a wide range of radionuclides with high resolution.
食物和水受到放射性物质污染是一个严重的健康问题。有多种方法可以检测和测量放射性物质,其中一些是近年来开发的。本文旨在讨论检测和测量食物和水中放射性物质的方法。讨论了每种方法的原理和优缺点。结果表明,其中一些方法,如γ射线高纯锗光谱法、便携式氡气测量仪 SILENA、RAD7 和电感耦合等离子体质谱法,具有较高的检测和测量灵敏度。此外,γ 射线高纯锗光谱法比其他方法更受关注,因为它能以高分辨率测量多种放射性核素。
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引用次数: 0
Detection of pathogenic Vibrio spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in bivalve mollusks and preliminary results 食品中致病性弧菌的检测:基于聚合酶链式反应的快速检测双壳软体动物中致病性副溶血性弧菌、霍乱弧菌和弧菌的筛选策略及初步结果
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-26 DOI: 10.4081/ijfs.2024.11635
Orlandina Di Maro, Y. Proroga, Silvia Castellano, Anna Balestrieri, Federico Capuano, Enrico Arletti, Michelangelo Vietina, Melissa Bizzarri, N. Murru, M. F. Peruzy, D. Cristiano
The majority of human diseases attributed to seafood are caused by Vibrio spp. and the most commonly reported species are Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae. The conventional methods for the detection of Vibrio species involve the use of selective media, which are inexpensive and simple but time-consuming. The present work aimed to develop a rapid method based on the use of multiplex real-time polymerase chain reaction (PCR) to detect V. parahaemolyticus, V. vulnificus, and V. cholerae in bivalve mollusks. 30 aliquots of bivalve mollusks (Mytilus galloprovincialis) were experimentally inoculated with two levels of V. parahaemolyticus, V. vulnificus, and V. cholerae. ISO 21872-1:2017 was used in parallel for qualitative analysis. The limit of detection of 50% was 7.67 CFU/g for V. cholerae, 0.024 CFU/g for V. vulnificus, and 1.36 CFU/g for V. parahaemolyticus. For V. vulnificus and V. cholerae, the real-time PCR protocol was demonstrated to amplify the pathogens in samples seeded with the lowest and highest levels. The molecular method evaluated showed a concordance rate of 100% with the reference microbiological method. V. parahaemolyticus was never detected in samples contaminated with the lowest level, and it was detected in 14 samples (93.33%) seeded with the highest concentration. In conclusion, the multiplex real-time PCR developed proved to be reliable for V. vulnificus and V. cholerae. Results for V. parahaemolyticus are promising, but further analysis is needed. The proposed method could represent a quick monitoring tool and, if used, would allow the implementation of food safety.
海鲜导致的人类疾病大多由弧菌引起,最常报道的弧菌种类是副溶血性弧菌、弧菌和霍乱弧菌。检测弧菌种类的传统方法涉及使用选择性培养基,这种方法成本低廉、操作简单,但耗费时间。本研究旨在开发一种基于多重实时聚合酶链反应(PCR)的快速方法,用于检测双壳类软体动物中的副溶血性弧菌、弧菌和霍乱弧菌。对 30 份双壳类软体动物(Mytilus galloprovincialis)的等分试样进行实验性接种,分别接种两种浓度的副溶血性弧菌、弧菌和霍乱弧菌。同时采用 ISO 21872-1:2017 进行定性分析。霍乱弧菌的 50%检出限为 7.67 CFU/g,弧菌为 0.024 CFU/g,副溶血性弧菌为 1.36 CFU/g。对于V. vulnificus和V. cholerae,实时PCR方案被证明能在含量最低和最高的种子样本中扩增病原体。所评估的分子方法与参考微生物方法的吻合率为 100%。在污染程度最低的样本中从未检测到副溶血性弧菌,而在污染程度最高的 14 个样本(93.33%)中检测到了副溶血性弧菌。总之,所开发的多重实时 PCR 被证明对弧菌和霍乱弧菌是可靠的。副溶血性弧菌的检测结果很有希望,但还需要进一步分析。所提出的方法可以作为一种快速监测工具,如果得到应用,将有助于食品安全的实施。
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引用次数: 0
Does hunted wild boar meat meet modern consumer nutritional expectations? 猎杀的野猪肉是否符合现代消费者的营养期望?
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-22 DOI: 10.4081/ijfs.2024.11608
Sara Di Bella, R. Branciari, N. Haouet, Marisa Framboas, M. L. Mercuri, Michela Codini, R. Roila, Alessandra Malimpensa, D. Ranucci
The present study aimed to evaluate the nutritional characteristics of hunted wild boar (WB) meat and compare them with those of meat from analogous domestic animals (pigs) reared in two different rearing systems: indoor-intensive (PI) and outdoor-extensive (PO). WB meat showed a lower amount of lipid content compared to pork and a higher antioxidant activity compared to PI and PO. The comparison of the fatty acid composition of WB and domestic pig reveals significant differences in saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA), with WB having the highest PUFA level and the lowest SFA. The omega 6/omega 3 (n-6/n-3) PUFA ratio, PUFA/SFA, atherogenic and thrombogenic indices (AI and TI), as well as the hypocholesterolemic/hypercholesterolemic index (h/H), were calculated. The n-6/n-3 PUFA ratio was higher in pork independently of the rearing system. The PUFA/SFA ratio of WB meat was above the minimum ratio of 0.40 recommended to contribute to a reduction in the risk of coronary diseases in pork from both rearing systems. AI and TI were lower in meat from hunted wild boars compared to commercially reared pigs, while h/H was higher in WB in comparison with pork meat. WB meat shows good nutritional quality; therefore, the use of game meat as a food source could be appropriate and could benefit contemporary consumers looking for “green” and high-nutritional products.
本研究旨在评估狩猎野猪(WB)肉的营养特性,并将其与在两种不同饲养系统(室内密集饲养系统(PI)和室外密集饲养系统(PO))下饲养的类似家畜(猪)肉的营养特性进行比较。与猪肉相比,WB 肉的脂质含量较低,而与 PI 和 PO 相比,WB 肉的抗氧化活性较高。通过比较 WB 和家猪的脂肪酸组成,发现饱和脂肪酸(SFA)和多不饱和脂肪酸(PUFA)存在显著差异,其中 WB 的多不饱和脂肪酸含量最高,而饱和脂肪酸含量最低。计算了ω 6/ω 3 (n-6/n-3) PUFA比率、PUFA/SFA、致动脉粥样硬化指数(AI)和致血栓指数(TI)以及低胆固醇血症/高胆固醇血症指数(h/H)。猪肉的 n-6/n-3 PUFA 比值较高,与饲养系统无关。WB肉的PUFA/SFA比率高于建议的最低比率0.40,这有助于降低两种饲养方式猪肉的冠心病风险。与商业化饲养的猪相比,狩猎野猪肉的AI和TI较低,而与猪肉相比,WB肉的h/H较高。野猪肉显示出良好的营养品质;因此,将野味肉用作食物来源是合适的,并能使追求 "绿色 "和高营养产品的当代消费者受益。
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引用次数: 0
Analysis of two cross-contamination cases of Campylobacter jejuni foodborne disease in fragile subjects in the territory of a Local Health Authority in Tuscany, Italy 意大利托斯卡纳地方卫生局辖区内两例脆弱人群交叉感染空肠弯曲菌食源性疾病病例分析
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-21 DOI: 10.4081/ijfs.2024.12053
Francesca Marconi, Martina Sartoni, Clara Girardi, Aurelio Rossi, Matteo Carrini, R. Nuvoloni, F. Pedonese, Giovanni Munaò
Campylobacteriosis is the most reported foodborne disease in the European Union, with more than 100,000 confirmed cases annually. Human infection can be caused by a low infectious dose, and in fragile populations, the food disease can manifest itself in acute and severe forms. This study aims to analyze two cases of campylobacteriosis in fragile people caused by Campylobacter jejuni in 2023 in Tuscany and the actions of the Local Health Competent Authority. From the results of the related investigations, it was possible to attribute both cases of foodborne diseases to unsafe food management during preparation/administration. Given the peculiar characteristics of the etiological agent, it is necessary to focus the attention of the population, especially those who deal with fragile subjects, on the good hygiene practices to be followed both at home and in collective catering.
弯曲杆菌病是欧盟报告最多的食源性疾病,每年确诊病例超过 10 万例。人体感染可由低感染剂量引起,在脆弱人群中,这种食源性疾病可表现为急性和严重形式。本研究旨在分析 2023 年托斯卡纳发生的两起由空肠弯曲杆菌引起的脆弱人群弯曲杆菌病病例,以及当地卫生主管部门采取的行动。根据相关调查的结果,可以将这两例食源性疾病归因于准备/管理过程中不安全的食品管理。鉴于这种病原体的特殊性,有必要让人们,尤其是那些与脆弱人群打交道的人,重点关注在家庭和集体餐饮中应遵循的良好卫生习惯。
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引用次数: 0
Development of a new method for extracting histamine from marine fish flesh using the salting-out technique 利用盐析技术开发从海洋鱼肉中提取组胺的新方法
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-19 DOI: 10.4081/ijfs.2024.12117
Charifa Aoua, B. Yacoubi, Abderrahmane Zekhnini
A simple and practical method was developed to extract histamine from fish products using sodium chloride (NaCl). After obtaining a saline extract from fish samples, histamine was derivatized by a condensation reaction with ortho-phthalaldehyde. Fluorescence intensity was measured by a fluorimeter. The first part of this work concerned a solid-liquid extraction tested with samples from the Food Analysis Performance Assessment Scheme. The best histamine extraction yield (97%) was obtained using an extraction time of 4 minutes, a temperature of 40°C, and a NaCl/water ratio of 41% (w/w). The second part focused on a liquid-liquid extraction carried out on standard solutions of histamine (45, 90, and 180 mg/kg). The use of NaCl (41%), and trichloroacetic acid [(TCA) 10%] did not show any significant difference in extraction yield. The yield obtained was 99.15-100.1% for TCA (10%) and 98.65-99.45% for NaCl (41%). The validation criteria (repeatability and reproducibility) were checked by evaluating the reliability of the method. Extraction using NaCl has proven to be an interesting alternative method for the extraction of histamine from fish, as it is reliable, inexpensive, and less hazardous.
利用氯化钠(NaCl)从鱼类产品中提取组胺的方法简单实用。从鱼肉样品中提取生理盐水后,组胺与邻苯二甲醛发生缩合反应而衍生化。荧光强度由荧光计测量。这项工作的第一部分是用食品分析性能评估计划的样品测试固液萃取。萃取时间为 4 分钟,温度为 40°C,氯化钠/水比例为 41%(重量比),组胺萃取率最高(97%)。第二部分的重点是对组胺标准溶液(45、90和180毫克/千克)进行液-液萃取。氯化钠(41%)和10%的三氯乙酸[(TCA)]在萃取率上没有明显差异。三氯乙酸(10%)的提取率为 99.15%-100.1%,氯化钠(41%)的提取率为 98.65%-99.45%。通过评估该方法的可靠性,检验了验证标准(重复性和再现性)。事实证明,使用氯化钠提取组胺是提取鱼肉中组胺的一种有趣的替代方法,因为它可靠、廉价且危害较小。
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引用次数: 0
Preliminary data on glyphosate, glufosinate, and metabolite contamination in Italian honey samples 关于意大利蜂蜜样品中草甘膦、草铵膦和代谢物污染的初步数据
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-13 DOI: 10.4081/ijfs.2024.11996
Giulia Rampazzo, E. Zironi, Giacomo Depau, G. Pagliuca, T. Gazzotti
Glyphosate and glufosinate are among the most widely used pesticides in agriculture worldwide. Their extensive use leads to the presence of their residues on crops and in the surrounding environment. Beehives, bees, and apiculture products can represent potential sources for the accumulation of these substances and their metabolites, and the consequences for bee health, as well as the level of risk to human health from consuming contaminated food, are still unclear. Furthermore, information on the contamination levels of honey and other beehive products by these compounds remains poorly documented. This study is part of a broader research effort aimed at developing specific analytical methods for monitoring the level of these contaminants in bee products. The methodology employed enabled the acquisition of preliminary information concerning the levels of glyphosate and glufosinate contamination in honey samples obtained from various retailers in Italy to assess compliance with the limits established by Regulation 293/2013. The liquid chromatography tandem mass spectrometry analysis of the 30 honey samples revealed quantifiable levels of glyphosate in eight samples, with contamination ranging from 5.4 to 138.5 ng/g. Notably, one sample of the wildflower type showed residue levels nearly three times the maximum residue limit. Additionally, trace levels of glyphosate contamination were detected in another ten samples. It is noteworthy that glufosinate and its metabolites were not detected in any of the analyzed samples within the established method’s detection ranges.
草甘膦和草铵膦是全世界农业中使用最广泛的农药之一。草甘膦和草铵膦的广泛使用导致其残留物出现在农作物和周围环境中。蜂箱、蜜蜂和养蜂产品可能是这些物质及其代谢物积累的潜在来源,其对蜜蜂健康的影响以及食用受污染食物对人类健康造成的风险程度尚不清楚。此外,有关蜂蜜和其他蜂巢产品受这些化合物污染程度的资料仍然很少。这项研究是更广泛研究工作的一部分,旨在开发监测蜂产品中这些污染物含量的特定分析方法。所采用的方法能够获得有关从意大利不同零售商处获得的蜂蜜样品中草甘膦和草铵膦污染水平的初步信息,以评估是否符合第 293/2013 号法规规定的限值。对 30 个蜂蜜样本进行的液相色谱串联质谱分析表明,8 个样本中的草甘膦含量可量化,污染范围在 5.4 至 138.5 纳克/克之间。值得注意的是,有一个野花类型的样品中的草甘膦残留量几乎是最高残留限量的三倍。此外,在另外 10 个样本中也检测到了微量草甘膦污染。值得注意的是,在既定方法的检测范围内,没有在任何分析样本中检测到草铵膦及其代谢物。
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引用次数: 0
Preliminary data on glyphosate, glufosinate, and metabolite contamination in Italian honey samples 关于意大利蜂蜜样品中草甘膦、草铵膦和代谢物污染的初步数据
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-13 DOI: 10.4081/ijfs.2024.11996
Giulia Rampazzo, E. Zironi, Giacomo Depau, G. Pagliuca, T. Gazzotti
Glyphosate and glufosinate are among the most widely used pesticides in agriculture worldwide. Their extensive use leads to the presence of their residues on crops and in the surrounding environment. Beehives, bees, and apiculture products can represent potential sources for the accumulation of these substances and their metabolites, and the consequences for bee health, as well as the level of risk to human health from consuming contaminated food, are still unclear. Furthermore, information on the contamination levels of honey and other beehive products by these compounds remains poorly documented. This study is part of a broader research effort aimed at developing specific analytical methods for monitoring the level of these contaminants in bee products. The methodology employed enabled the acquisition of preliminary information concerning the levels of glyphosate and glufosinate contamination in honey samples obtained from various retailers in Italy to assess compliance with the limits established by Regulation 293/2013. The liquid chromatography tandem mass spectrometry analysis of the 30 honey samples revealed quantifiable levels of glyphosate in eight samples, with contamination ranging from 5.4 to 138.5 ng/g. Notably, one sample of the wildflower type showed residue levels nearly three times the maximum residue limit. Additionally, trace levels of glyphosate contamination were detected in another ten samples. It is noteworthy that glufosinate and its metabolites were not detected in any of the analyzed samples within the established method’s detection ranges.
草甘膦和草铵膦是全世界农业中使用最广泛的农药之一。草甘膦和草铵膦的广泛使用导致其残留物出现在农作物和周围环境中。蜂箱、蜜蜂和养蜂产品可能是这些物质及其代谢物积累的潜在来源,其对蜜蜂健康的影响以及食用受污染食物对人类健康造成的风险程度尚不清楚。此外,有关蜂蜜和其他蜂巢产品受这些化合物污染程度的资料仍然很少。这项研究是更广泛研究工作的一部分,旨在开发监测蜂产品中这些污染物含量的特定分析方法。所采用的方法能够获得有关从意大利不同零售商处获得的蜂蜜样品中草甘膦和草铵膦污染水平的初步信息,以评估是否符合第 293/2013 号法规规定的限值。对 30 个蜂蜜样本进行的液相色谱串联质谱分析表明,8 个样本中的草甘膦含量可量化,污染范围在 5.4 至 138.5 纳克/克之间。值得注意的是,有一个野花类型的样品中的草甘膦残留量几乎是最高残留限量的三倍。此外,在另外 10 个样本中也检测到了微量草甘膦污染。值得注意的是,在既定方法的检测范围内,没有在任何分析样本中检测到草铵膦及其代谢物。
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引用次数: 0
Analyzing consumer perceptions about food safety by applying the food-related lifestyle approach 运用食品相关生活方式方法分析消费者对食品安全的看法
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-05 DOI: 10.4081/ijfs.2024.11315
Iliriana Miftari, D. Imami, Sina Ahmadi Kaliji, M. Canavari, E. Gjokaj
Food safety standards remain a major concern for consumers, especially in developing and emerging economies, due to gaps in the capacities of food safety enforcement institutions and limited investments from private sector actors. This study analyzes consumer behavior and food safety-related lifestyles in Kosovo, a post-communist emerging economy. Consumer groups were identified according to their food- related lifestyle using the classical 2-stage segmentation approach of factor analysis as well as cluster analysis. The study findings indicate that almost 2/3 of the surveyed consumers are fairly or very concerned about food safety. The results reveal that highly educated female consumers are more concerned about personal health risks, food safety-related content, and related diseases. This study provides interesting insights for food safety policymakers as well as the agroindustry on how to promote food safety consumer awareness effectively with targeted communication strategies.
由于食品安全执法机构的能力不足以及私营部门的投资有限,食品安全标准仍然是消费者关注的主要问题,尤其是在发展中国家和新兴经济体。本研究分析了后共产主义新兴经济体科索沃的消费者行为和与食品安全相关的生活方式。根据消费者与食品相关的生活方式,采用经典的两阶段细分方法进行因素分析和聚类分析,确定了消费者群体。研究结果表明,近三分之二的受访消费者相当或非常关注食品安全。结果显示,受过高等教育的女性消费者更关注个人健康风险、食品安全相关内容和相关疾病。这项研究为食品安全政策制定者和农业产业提供了有趣的见解,帮助他们了解如何通过有针对性的传播策略有效提高消费者的食品安全意识。
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引用次数: 0
Analyzing consumer perceptions about food safety by applying the food-related lifestyle approach 运用食品相关生活方式方法分析消费者对食品安全的看法
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-02-05 DOI: 10.4081/ijfs.2024.11315
Iliriana Miftari, D. Imami, Sina Ahmadi Kaliji, M. Canavari, E. Gjokaj
Food safety standards remain a major concern for consumers, especially in developing and emerging economies, due to gaps in the capacities of food safety enforcement institutions and limited investments from private sector actors. This study analyzes consumer behavior and food safety-related lifestyles in Kosovo, a post-communist emerging economy. Consumer groups were identified according to their food- related lifestyle using the classical 2-stage segmentation approach of factor analysis as well as cluster analysis. The study findings indicate that almost 2/3 of the surveyed consumers are fairly or very concerned about food safety. The results reveal that highly educated female consumers are more concerned about personal health risks, food safety-related content, and related diseases. This study provides interesting insights for food safety policymakers as well as the agroindustry on how to promote food safety consumer awareness effectively with targeted communication strategies.
由于食品安全执法机构的能力不足以及私营部门的投资有限,食品安全标准仍然是消费者关注的主要问题,尤其是在发展中国家和新兴经济体。本研究分析了后共产主义新兴经济体科索沃的消费者行为和与食品安全相关的生活方式。根据消费者与食品相关的生活方式,采用经典的两阶段细分方法进行因素分析和聚类分析,确定了消费者群体。研究结果表明,近三分之二的受访消费者相当或非常关注食品安全。结果显示,受过高等教育的女性消费者更关注个人健康风险、食品安全相关内容和相关疾病。这项研究为食品安全政策制定者和农业产业提供了有趣的见解,帮助他们了解如何通过有针对性的传播策略有效提高消费者的食品安全意识。
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引用次数: 0
Assessment of adoption of Global GAP standard: Evidences from Fruit and Vegetable farmers 评估全球 GAP 标准的采用情况:来自果农和菜农的证据
IF 1.3 Q3 Agricultural and Biological Sciences Pub Date : 2024-01-26 DOI: 10.4081/ijfs.2024.12144
Syed Raza Hussain, Muhammad Zahid Rashid, Irfan Haidri, Usman Shafqat, Faisal Mahmood
Food safety certifications, such as the Global Good Agricultural practices standard (GGAP), are mandatory for export. This research was conducted as a baseline to study the suitability of this standard in the generic conditions of Pakistan. A questionnaire was prepared based on the Fruit and Vegetable (F&V) module of GGAP. Based on annual turnover, five (5) groups were designed comprising fifteen (15) farmers. Results revealed that the clauses related to simple paperwork were found implementable. However, clauses that needed capital investment and technical records, etc., were declared as not implementable. Results also revealed that a significant number of farmers favoured declaring risk assessment, training, and documents as implementable. Results of record keeping, installation, visual presentation, and infrastructure showed that 42-56% of clauses were implementable. Moreover, in the case of external testing, health safety, and hygiene, 24-37 % of clauses were found to be not implementable. So, based on the results, it can be concluded that a major number of the Global GAP standard clauses were found unsuitable or not implementable in the generic conditions of Pakistan, and there is a dire need to harmonize clauses to create local standards.
食品安全认证,如全球良好农业规范标准(GGAP),是出口的强制性认证。本研究以巴基斯坦的一般条件为基线,研究该标准的适用性。根据 GGAP 的水果和蔬菜(F&V)模块编制了一份调查问卷。根据年营业额,设计了五(5)个小组,由十五(15)位农民组成。结果表明,与简单文书工作有关的条款可以实施。但是,需要资本投资和技术记录等的条款被宣布为不可实施。结果还显示,相当多的农民赞成将风险评估、培训和文件列为可实施条款。记录保存、安装、直观演示和基础设施方面的结果显示,42%-56%的条款可以实施。此外,在外部检测、健康安全和卫生方面,24%-37% 的条款被认为不可实施。因此,根据调查结果可以得出结论,全球良好农业规范标准的大部分条款不适合或无法在巴基斯坦的一般条件下实施,因此亟需对条款进行协调,以制定本地标准。
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引用次数: 0
期刊
Italian Journal of Food Safety
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