Jesús Elías Dawaher Dawaher, Rafael Salazar Montesdeoca, Santiago Aguayo-Moscoso, Wendy Bonilla Poma, J. Vélez-Páez
The massive prescription of antimicrobials accelerated the gen-eration of multi-resistant bacteria during the SARS-CoV-2 pandemic. This work aims to present the epidemiological, clinical, and microbiological profiles of a series of patients with bacterial superinfections hospitalized in a COVID-19 reference center. We conducted a retrospective observational study in adult COVID-19 patients hospitalized between January and December 2021 who pre-sented with bacterial superinfections. Mortality at discharge was the variable outcome. The median age of the 240 patients included in the study was 55 years, and the male sex predominated at 68.75%. The median stay of hospi-talization was 24 days. Superinfections occurred in 55% of patients with me-chanical ventilation. The most frequent bacteria were KPC-producing Klebsiella pneumoniaecomplex (24.17%), ESBL -producing Klebsiella pneumoniae com-plex (17.92%), and carbapenem-resistant Pseudomonas aeruginosa (13.75%). The most used empirical and targeted antibiotic schemes consisted of the asso-ciation of carbapenem, glycopeptides, and aminoglycosides (56.09 and 38.55%, respectively). In the multivariate analysis, older age (p= 0.006, OR 1.03, 95% CI: 1.01-1.06), central venous catheter-related bacteremia (CLBSI) (p= 0.028, OR 1.94, 95%CI: 1.07-3.49), and the use of colistin associated with other anti-biotics as targeted therapy (p: 0.028, OR 12, 95%CI: 1.30-110.52), were inde-pendent predictors of mortality. In this series, we found that in patients with COVID-19 and bacterial superinfection, age, CLBSI, and colistin use were in-dependent predictors of non-survival. The most frequently isolated microor-ganisms were ESBL - and KPC-producing enterobacterales and non-fermenting Gram-negative bacilli resistant to carbapenems.
{"title":"COVID-19 and bacterial superinfections: clinical and microbiological profiles, and determinants of mortality in a reference center in Quito, Ecuador.","authors":"Jesús Elías Dawaher Dawaher, Rafael Salazar Montesdeoca, Santiago Aguayo-Moscoso, Wendy Bonilla Poma, J. Vélez-Páez","doi":"10.54817/ic.v64n3a08","DOIUrl":"https://doi.org/10.54817/ic.v64n3a08","url":null,"abstract":"The massive prescription of antimicrobials accelerated the gen-eration of multi-resistant bacteria during the SARS-CoV-2 pandemic. This work aims to present the epidemiological, clinical, and microbiological profiles of a series of patients with bacterial superinfections hospitalized in a COVID-19 reference center. We conducted a retrospective observational study in adult COVID-19 patients hospitalized between January and December 2021 who pre-sented with bacterial superinfections. Mortality at discharge was the variable outcome. The median age of the 240 patients included in the study was 55 years, and the male sex predominated at 68.75%. The median stay of hospi-talization was 24 days. Superinfections occurred in 55% of patients with me-chanical ventilation. The most frequent bacteria were KPC-producing Klebsiella pneumoniaecomplex (24.17%), ESBL -producing Klebsiella pneumoniae com-plex (17.92%), and carbapenem-resistant Pseudomonas aeruginosa (13.75%). The most used empirical and targeted antibiotic schemes consisted of the asso-ciation of carbapenem, glycopeptides, and aminoglycosides (56.09 and 38.55%, respectively). In the multivariate analysis, older age (p= 0.006, OR 1.03, 95% CI: 1.01-1.06), central venous catheter-related bacteremia (CLBSI) (p= 0.028, OR 1.94, 95%CI: 1.07-3.49), and the use of colistin associated with other anti-biotics as targeted therapy (p: 0.028, OR 12, 95%CI: 1.30-110.52), were inde-pendent predictors of mortality. In this series, we found that in patients with COVID-19 and bacterial superinfection, age, CLBSI, and colistin use were in-dependent predictors of non-survival. The most frequently isolated microor-ganisms were ESBL - and KPC-producing enterobacterales and non-fermenting Gram-negative bacilli resistant to carbapenems.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49439355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carlos Álvarez, Andrea Bullones, María Medina, Anna Vargas, A. Porco, Juan Méndez, C. Pestana
Aging is an irreversible process that produces the progressive decline of physiological functions favoring the development of cardiovascular complications associated with genetic Risk Alleles (RA). A case-control study using a sample of 90 Venezuelan individuals was performed to determine the correlation between the incidence of accelerated aging for 14 polymorphisms in genes associated with blood coagulation, lipid, and cardiovascular homeostasis. Odds Ratio (OR) results showed a 41% increase in the risk of presenting accel-erated aging in subjects with the rs1800790 RA in the FGB gene. The CC geno-type for the rs1800775 in the CETP gene was associated with a 62%, and the TT genotype for the rs1801133 in the MTHFR gene increased risk by two times. However, none of these results were statistically significant. Only a significant association was determined between the presence of the homozygous deletion genotype for the rs4340 RA in the ACE gene with an increased risk up to ten times (OR: 10.6; CI: 1.23 - 90.67; p<0.05). Multivariable analyses showed that gender, obesity, hypercholesterolemia, hypertriglyceridemia, smoking, age, body mass index, systolic hypertension, the rs662 RA in the APOB, rs693 RA in the PON1 and rs1801133 RA in the MTHFR genes were the main environmental and genetic factors associated with accelerated aging.
{"title":"Relationships between genetic vascular risk polymorphism and aging. A case-control study in Venezuela.","authors":"Carlos Álvarez, Andrea Bullones, María Medina, Anna Vargas, A. Porco, Juan Méndez, C. Pestana","doi":"10.54817/ic.v64n3a02","DOIUrl":"https://doi.org/10.54817/ic.v64n3a02","url":null,"abstract":"Aging is an irreversible process that produces the progressive decline of physiological functions favoring the development of cardiovascular complications associated with genetic Risk Alleles (RA). A case-control study using a sample of 90 Venezuelan individuals was performed to determine the correlation between the incidence of accelerated aging for 14 polymorphisms in genes associated with blood coagulation, lipid, and cardiovascular homeostasis. Odds Ratio (OR) results showed a 41% increase in the risk of presenting accel-erated aging in subjects with the rs1800790 RA in the FGB gene. The CC geno-type for the rs1800775 in the CETP gene was associated with a 62%, and the TT genotype for the rs1801133 in the MTHFR gene increased risk by two times. However, none of these results were statistically significant. Only a significant association was determined between the presence of the homozygous deletion genotype for the rs4340 RA in the ACE gene with an increased risk up to ten times (OR: 10.6; CI: 1.23 - 90.67; p<0.05). Multivariable analyses showed that gender, obesity, hypercholesterolemia, hypertriglyceridemia, smoking, age, body mass index, systolic hypertension, the rs662 RA in the APOB, rs693 RA in the PON1 and rs1801133 RA in the MTHFR genes were the main environmental and genetic factors associated with accelerated aging.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48293292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chunhong Lin, Jianyong Chen, Xianguo Li, Linlin Wang, Fengqin Yan, Ye Chang, Xueniu Yang
We aimed to explore the clinical value of four-dimensional hys-terosalpingo-contrast sonography (4D-HyCoSy) assisted by intrauterine pres-sure measurement for evaluating tubal patency. One hundred and thirty-two patients diagnosed with tubal factor infertility from February 2018 to February 2021 were selected as subjects. With hysterosalpingography diagnosis results as the gold standard, 4D-HyCoSy was conducted for all patients, and the status of the fallopian tubes was classified into patency, occlusion, and partial occlusion. Based on the function of fallopian tubes, 4D-HyCoSy diagnosis results revealed that fallopian tubes showed bilateral patency, incomplete patency (including bilateral partial occlusion, unilateral patency, and unilateral partial occlusion, unilateral patency and unilateral occlusion), unilateral partial occlusion and unilateral occlusion, and bilateral occlusion. Thecutoff value of peak intra-uterine pressure was determined using the receiver operating characteristic curve (ROC), specificity, and the area under the ROC curve (AUC) between 4D-HyCoSy alone and 4D-HyCoSy assisted by intrauterine pressure measure-ments. There were significant differences in the peak intrauterine pressure among patients with bilateral patency, incomplete patency, unilateral partial occlusion, and unilateral and bilateral occlusions (p<0.05). The corresponding cutoff values of peak intrauterine pressure were 24.42, 36.34, and 47.68 kPa; AUC values were 0.812, 0.836, and 0.827, respectively. The FSM model showed that the AUC of 4D-HyCoSy alone, assisted by peak intrauterine pressure was 0.85, with a higher sensitivity (88.13%) than that of 4D-HyCoSy (p<0.05). 4D-HyCoSy, assisted by intrauterine pressure measurement, has an excellent value for evaluating tubal patency.
{"title":"Clinical value of four-dimensional hysterosalpingo-contrast sonography assisted by intrauterine pressure measurement for tubal patency evaluation","authors":"Chunhong Lin, Jianyong Chen, Xianguo Li, Linlin Wang, Fengqin Yan, Ye Chang, Xueniu Yang","doi":"10.54817/ic.v64n3a05","DOIUrl":"https://doi.org/10.54817/ic.v64n3a05","url":null,"abstract":"We aimed to explore the clinical value of four-dimensional hys-terosalpingo-contrast sonography (4D-HyCoSy) assisted by intrauterine pres-sure measurement for evaluating tubal patency. One hundred and thirty-two patients diagnosed with tubal factor infertility from February 2018 to February 2021 were selected as subjects. With hysterosalpingography diagnosis results as the gold standard, 4D-HyCoSy was conducted for all patients, and the status of the fallopian tubes was classified into patency, occlusion, and partial occlusion. Based on the function of fallopian tubes, 4D-HyCoSy diagnosis results revealed that fallopian tubes showed bilateral patency, incomplete patency (including bilateral partial occlusion, unilateral patency, and unilateral partial occlusion, unilateral patency and unilateral occlusion), unilateral partial occlusion and unilateral occlusion, and bilateral occlusion. Thecutoff value of peak intra-uterine pressure was determined using the receiver operating characteristic curve (ROC), specificity, and the area under the ROC curve (AUC) between 4D-HyCoSy alone and 4D-HyCoSy assisted by intrauterine pressure measure-ments. There were significant differences in the peak intrauterine pressure among patients with bilateral patency, incomplete patency, unilateral partial occlusion, and unilateral and bilateral occlusions (p<0.05). The corresponding cutoff values of peak intrauterine pressure were 24.42, 36.34, and 47.68 kPa; AUC values were 0.812, 0.836, and 0.827, respectively. The FSM model showed that the AUC of 4D-HyCoSy alone, assisted by peak intrauterine pressure was 0.85, with a higher sensitivity (88.13%) than that of 4D-HyCoSy (p<0.05). 4D-HyCoSy, assisted by intrauterine pressure measurement, has an excellent value for evaluating tubal patency.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45071418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruijin Song, Li Feng, Xueyi Liu, Bo Yang, M. Gong, Tie Qiao
Abstract. This study aimed to determine the efficacy and safety of a mini-mally invasive combined approach for concomitant stone clearance of choleli-thiasis and choledocholithiasis. FromNovember 2018 to March 2021, 30 pa-tients were enrolled in this retrospective study that compared two methods of treating combined cholelithiasis and choledocholithiasis. The study comprised two groups: 15 patients in Group A underwent combined laparoscopic and Seldinger techniques for complete stone clearance, retaining the gall bladder in situ. In Group B, 15 patients underwent laparoscopic cholecystectomy and choledocholithotomy with T-tube drainage. The rates of successful completion of the operations, procedure-related complications, length of hospitalization, hospital cost, and patient satisfaction were compared between the two groups. The two groups had no differences in general patient characteristics, and all procedures were successfully completed. Compared to Group B, patients in Group A had a shorter operative time (84 vs. 105 min), less blood loss (10 vs. 28 mL), were less expensive, and had a shorter postoperative recovery. A single patient in group B developed bile leakage. The satisfaction rate was 93% in Group A, in contrast to 80% in Group B. The combined use of laparoscopic and Seldinger techniques to achieve complete stone removal in patients with con-comitant cholelithiasis and choledocholithiasis was demonstrated to be safe and successful.
{"title":"Laparoscopic and Seldinger techniques for the treatment of concomitant gallstones and choledocholithiasis. A retrospective study.","authors":"Ruijin Song, Li Feng, Xueyi Liu, Bo Yang, M. Gong, Tie Qiao","doi":"10.54817/ic.v64n2a03","DOIUrl":"https://doi.org/10.54817/ic.v64n2a03","url":null,"abstract":"Abstract. This study aimed to determine the efficacy and safety of a mini-mally invasive combined approach for concomitant stone clearance of choleli-thiasis and choledocholithiasis. FromNovember 2018 to March 2021, 30 pa-tients were enrolled in this retrospective study that compared two methods of treating combined cholelithiasis and choledocholithiasis. The study comprised two groups: 15 patients in Group A underwent combined laparoscopic and Seldinger techniques for complete stone clearance, retaining the gall bladder in situ. In Group B, 15 patients underwent laparoscopic cholecystectomy and choledocholithotomy with T-tube drainage. The rates of successful completion of the operations, procedure-related complications, length of hospitalization, hospital cost, and patient satisfaction were compared between the two groups. The two groups had no differences in general patient characteristics, and all procedures were successfully completed. Compared to Group B, patients in Group A had a shorter operative time (84 vs. 105 min), less blood loss (10 vs. 28 mL), were less expensive, and had a shorter postoperative recovery. A single patient in group B developed bile leakage. The satisfaction rate was 93% in Group A, in contrast to 80% in Group B. The combined use of laparoscopic and Seldinger techniques to achieve complete stone removal in patients with con-comitant cholelithiasis and choledocholithiasis was demonstrated to be safe and successful.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45162377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Early diagnosis and treatment are vital to improving lung cancer patients’ quality of life and survival rate. This study aimed to investigate the value of dynamic enhanced scanning examination by computed tomography (CT) in early lung cancer diagnosis. One hundred and twenty patients with isolated lung nodules were selected to analyze this diagnostic method, using pathological diagnostic results of cancer as the gold standard. Of the 120 pa-tients with isolated pulmonary nodules, the diagnosis was confirmed by patho-logical examination in 96 patients with early lung cancer (adenocarcinoma of the lung) and 24 patients with benign lung lesions. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of CT dynamic enhancement scans for the diagnosis of early-stage lung cancer were 93.75%, 83.33%, 91.67%, 95.74%, and 76.92%, respectively. Early-stage lung cancer had significantly less blood volume and a noticeably shorter mean time to passage than benign lung lesions (p<0.01). Blood flow and surface permeability were higher in early-stage lung cancer than in benign lung lesions (p<0.05). The ar-eas under the receiver operating characteristic (ROC) curves for blood volume, blood flow, surface permeability, and mean time to passage for the diagnosis of early-stage lung cancer were 0.737, 0.724, 0.779, and 0.946, respectively. In conclusion, CT dynamic enhancement scan has good application value in diag-nosing early lung cancer and is worth promoting in clinical practice.
{"title":"Analysis of the value of dynamic computed tomography (CT) examination in the diagnosis of early lung cancer.","authors":"Liang Sheng, Liang Wu, X. Xia, Junmiao Li","doi":"10.54817/ic.v64n2a01","DOIUrl":"https://doi.org/10.54817/ic.v64n2a01","url":null,"abstract":"Abstract. Early diagnosis and treatment are vital to improving lung cancer patients’ quality of life and survival rate. This study aimed to investigate the value of dynamic enhanced scanning examination by computed tomography (CT) in early lung cancer diagnosis. One hundred and twenty patients with isolated lung nodules were selected to analyze this diagnostic method, using pathological diagnostic results of cancer as the gold standard. Of the 120 pa-tients with isolated pulmonary nodules, the diagnosis was confirmed by patho-logical examination in 96 patients with early lung cancer (adenocarcinoma of the lung) and 24 patients with benign lung lesions. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of CT dynamic enhancement scans for the diagnosis of early-stage lung cancer were 93.75%, 83.33%, 91.67%, 95.74%, and 76.92%, respectively. Early-stage lung cancer had significantly less blood volume and a noticeably shorter mean time to passage than benign lung lesions (p<0.01). Blood flow and surface permeability were higher in early-stage lung cancer than in benign lung lesions (p<0.05). The ar-eas under the receiver operating characteristic (ROC) curves for blood volume, blood flow, surface permeability, and mean time to passage for the diagnosis of early-stage lung cancer were 0.737, 0.724, 0.779, and 0.946, respectively. In conclusion, CT dynamic enhancement scan has good application value in diag-nosing early lung cancer and is worth promoting in clinical practice.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45564078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cristina Royero-León, Mayira Sojo-Milano, Ligia Pérez-Castro, Dionelys Barazarte-Sánchez, J. Golaszewski, Rubén Muñoz-Gelvez, Fernando Gómez-Daza
Abstract. Mucormycosis is an emerging, severe, opportunistic, invasive mycosis with a worldwide distribution, low frequency, and high morbidity and mortality linked to immunodeficiency conditions. A significant increase in COVID-19-associated mucormycosis (CAM) has occurred in countries such as India and several countries of the Americas. We describe the presentation and management of a CAM case and discuss the clinical and public health sig-nificance of its characteristics concerning CAM and non-CAM cases reported in the literature. Using a timeline, epidemiological, clinical, microbiological, histopathological, imaging features, and multidisciplinary interventions in this CAM case are presented. A 64-year-old male patient from the north cen-ter of Venezuela, with a history of diabetes mellitus and a home-cared mod-erate COVID-19 episode treated on an outpatient basis, presented with left unilateral rhino-sinus-orbital mucormycosis with dentomaxillary extension. His first symptoms and signs were sinusitis, palatal changes, maxillary region pain, and loosening of teeth 19 days after the COVID-19 diagnosis. Mycologi-cal diagnosis supported by histopathology, direct examination, and culture indicated a Rhizopus spp. infection. The patient was successfully treated with amphotericin B, surgery with an endoscopic approach, and metabolic control. We discuss the influence of COVID-19 and its management regarding CAM development, as well as the importance of access to healthcare in this situa-tion. Mucormycosis should be considered among potential complications in diabetic patients with COVID-19. Therefore, comprehensive care and moni-toring must be provided in all phases of the COVID-19 clinical picture.
{"title":"Mucormicosis asociada a la COVID-19 en Venezuela: consideraciones en torno a un caso y revisión de la literatura.","authors":"Cristina Royero-León, Mayira Sojo-Milano, Ligia Pérez-Castro, Dionelys Barazarte-Sánchez, J. Golaszewski, Rubén Muñoz-Gelvez, Fernando Gómez-Daza","doi":"10.54817/ic.v64n2a07","DOIUrl":"https://doi.org/10.54817/ic.v64n2a07","url":null,"abstract":"Abstract. Mucormycosis is an emerging, severe, opportunistic, invasive mycosis with a worldwide distribution, low frequency, and high morbidity and mortality linked to immunodeficiency conditions. A significant increase in COVID-19-associated mucormycosis (CAM) has occurred in countries such as India and several countries of the Americas. We describe the presentation and management of a CAM case and discuss the clinical and public health sig-nificance of its characteristics concerning CAM and non-CAM cases reported in the literature. Using a timeline, epidemiological, clinical, microbiological, histopathological, imaging features, and multidisciplinary interventions in this CAM case are presented. A 64-year-old male patient from the north cen-ter of Venezuela, with a history of diabetes mellitus and a home-cared mod-erate COVID-19 episode treated on an outpatient basis, presented with left unilateral rhino-sinus-orbital mucormycosis with dentomaxillary extension. His first symptoms and signs were sinusitis, palatal changes, maxillary region pain, and loosening of teeth 19 days after the COVID-19 diagnosis. Mycologi-cal diagnosis supported by histopathology, direct examination, and culture indicated a Rhizopus spp. infection. The patient was successfully treated with amphotericin B, surgery with an endoscopic approach, and metabolic control. We discuss the influence of COVID-19 and its management regarding CAM development, as well as the importance of access to healthcare in this situa-tion. Mucormycosis should be considered among potential complications in diabetic patients with COVID-19. Therefore, comprehensive care and moni-toring must be provided in all phases of the COVID-19 clinical picture.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45024795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. The present narrative review was conducted to investigate and to compile information about the physiopathology of the Human Papilloma Virus (HPV) and the viral mechanisms of infection of the host’s cells, as well as how the virus survives the host’s innate immunological mechanisms and the mechanisms to produce cervical benign and malignant lesions. Literature searches were performed electronically in PubMed, Medline, ISI, DOAJ, Spring-er, Embase. Web of Knowledge, DOAJ, y Google Scholar, Hinari, JAMA Network, Oxford Academic and Research Life for original articles written in English and Scielo, Lantidex, Imbiomed-L, Redalyc, and Google Scholar for original articles written in Spanish. The searches included the keywords (MESH): physiopathol-ogy of HPV, HPV viral cycle, Carcinogenesis of HPV, HPV genomic structure, infection mechanism, and HPV taxonomy. Publications from January 1985 to August 2021 were reviewed. This narrative review allows us to understand how HPV produces productive and non-productive infection in the cells of the strati-fied squamous epithelium of the human being, especially that of the cervix, ex-plains how infection by the virus can produce benign lesions and malignant le-sions and explains why they are classified as HPV-HR and HPV-LR, according to their oncogenic capacity. These processes have made it possible to understand the behavior of the virus and establish primary treatment for the prevention of cervical cancer.
摘要本综述旨在调查和整理关于人乳头瘤病毒(HPV)的生理病理和感染宿主细胞的病毒机制，以及病毒如何在宿主的先天免疫机制中存活，以及产生宫颈良性和恶性病变的机制。文献检索在PubMed, Medline, ISI, DOAJ, Spring-er, Embase进行电子检索。Web of Knowledge、DOAJ、谷歌Scholar、Hinari、JAMA Network、Oxford Academic and Research Life的英文原创文章，以及Scielo、Lantidex、Imbiomed-L、Redalyc和谷歌Scholar的西班牙文原创文章。关键词(MESH): HPV的生理病理、HPV病毒周期、HPV的癌变、HPV的基因组结构、感染机制和HPV的分类。回顾了1985年1月至2021年8月的出版物。这篇叙述性综述使我们了解了HPV如何在人类的分层鳞状上皮细胞中产生生产性和非生产性感染，特别是子宫颈细胞，解释了病毒感染如何产生良性病变和恶性病变，并解释了为什么根据其致癌能力将它们分类为HPV- hr和HPV- lr。这些过程使人们有可能了解病毒的行为，并确定预防宫颈癌的初级治疗方法。
{"title":"Papel del virus del papiloma humano en el desarrollo del cáncer del cuello uterino.","authors":"José Núñez-Troconis","doi":"10.54817/ic.v64n2a09","DOIUrl":"https://doi.org/10.54817/ic.v64n2a09","url":null,"abstract":"Abstract. The present narrative review was conducted to investigate and to compile information about the physiopathology of the Human Papilloma Virus (HPV) and the viral mechanisms of infection of the host’s cells, as well as how the virus survives the host’s innate immunological mechanisms and the mechanisms to produce cervical benign and malignant lesions. Literature searches were performed electronically in PubMed, Medline, ISI, DOAJ, Spring-er, Embase. Web of Knowledge, DOAJ, y Google Scholar, Hinari, JAMA Network, Oxford Academic and Research Life for original articles written in English and Scielo, Lantidex, Imbiomed-L, Redalyc, and Google Scholar for original articles written in Spanish. The searches included the keywords (MESH): physiopathol-ogy of HPV, HPV viral cycle, Carcinogenesis of HPV, HPV genomic structure, infection mechanism, and HPV taxonomy. Publications from January 1985 to August 2021 were reviewed. This narrative review allows us to understand how HPV produces productive and non-productive infection in the cells of the strati-fied squamous epithelium of the human being, especially that of the cervix, ex-plains how infection by the virus can produce benign lesions and malignant le-sions and explains why they are classified as HPV-HR and HPV-LR, according to their oncogenic capacity. These processes have made it possible to understand the behavior of the virus and establish primary treatment for the prevention of cervical cancer.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45476229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Delia Buitrago, Susan Rojas-Rivas, Aguasanta González-Delatorre, Guillermo Terán-Ángel
Abstract. Amyand’s hernia is an inguinal hernia that contains the cecal appendix inside. It is a rare entity and its discovery is generally made intraop-eratively. We present the case of a older male adult, with a soft tumor, with pro-gressive increase in volume, in the right inguinal region accompanied by local pain, reducible at rest. During surgery, a hernial sac was found with an enlarged cecal appendix inside it, with signs of inflammation. An appendectomy was per-formed. The hernia sac was reintroduced and the posterior wall of the inguinal canal was repaired (Lichtenstein and Bassini mixed technique). Postoperative evolution was satisfactory.
{"title":"Hallazgo intraoperatorio de hernia de Amyand: reporte de caso y revisión de literatura.","authors":"Delia Buitrago, Susan Rojas-Rivas, Aguasanta González-Delatorre, Guillermo Terán-Ángel","doi":"10.54817/ic.v64n2a08","DOIUrl":"https://doi.org/10.54817/ic.v64n2a08","url":null,"abstract":"Abstract. Amyand’s hernia is an inguinal hernia that contains the cecal appendix inside. It is a rare entity and its discovery is generally made intraop-eratively. We present the case of a older male adult, with a soft tumor, with pro-gressive increase in volume, in the right inguinal region accompanied by local pain, reducible at rest. During surgery, a hernial sac was found with an enlarged cecal appendix inside it, with signs of inflammation. An appendectomy was per-formed. The hernia sac was reintroduced and the posterior wall of the inguinal canal was repaired (Lichtenstein and Bassini mixed technique). Postoperative evolution was satisfactory.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44594806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xingkui Tang, Yukun Lin, Yaqiong Wang, Jialin He, Xijun Luo, Jun Jie Liang, Xianjun Zhu
Abstract. We investigated the expression of micro ribonucleic acid (miR)-20a-5p and its target gene, breast cancer metastasis suppressor 1 like (BRMS1L), in colon cancer tissues and their effects on the proliferation and apoptosis of colon cancer cells. The dual luciferase assay was used to detect the targeted regulation of miR-20a-5p on BRMS1L. The expression levels of miR-20a-5p and BRMS1L in colon cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). MiR-20a-5p mimic and mimic negative control (NC) were transfected into the colon cancer cell line SW480 by the liposome transient transfection method. The MTT assay, monoclonal formation of cancer cells, and flow cytometry were used to detect cell proliferation and apoptosis. The expres-sion level of miR-20a-5p in colon cancer tissues was significantly higher than that in adjacent tissues, and the expression level of BRMS1L was significantly lower than that in adjacent tissues. The expression level of miR-20a-5p was significantly correlated with tumor-node-metastasis (TNM) stage, lymph node metastasis, in-vasion depth, and differentiation degree. The higher the expression level of miR-20a-5p, the more advanced the TNM stage and invasion depth, and the easier it is for lymph nodes to metastasize (p<0.05). Compared with the control and the miR-NC groups, the miR-20a-5p group’s cell proliferation ability, expression of CyclinD1 and B-cell lymphoma-2 (Bcl-2) were significantly increased, while apoptosis ability and caspase-3 protein expression were significantly decreased (p<0.05). The expression of miR-20a-5p in colon cancer tissues and cells in-creased. Overexpression of miR-20a-5p could promote the proliferation of colon cancer cells and inhibit their apoptosis.
{"title":"Expression of MiR-20a-5p and its target gene in colon cancer and its effect on the proliferation and apoptosis of colon cancer cells.","authors":"Xingkui Tang, Yukun Lin, Yaqiong Wang, Jialin He, Xijun Luo, Jun Jie Liang, Xianjun Zhu","doi":"10.54817/ic.v64n2a05","DOIUrl":"https://doi.org/10.54817/ic.v64n2a05","url":null,"abstract":"Abstract. We investigated the expression of micro ribonucleic acid (miR)-20a-5p and its target gene, breast cancer metastasis suppressor 1 like (BRMS1L), in colon cancer tissues and their effects on the proliferation and apoptosis of colon cancer cells. The dual luciferase assay was used to detect the targeted regulation of miR-20a-5p on BRMS1L. The expression levels of miR-20a-5p and BRMS1L in colon cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). MiR-20a-5p mimic and mimic negative control (NC) were transfected into the colon cancer cell line SW480 by the liposome transient transfection method. The MTT assay, monoclonal formation of cancer cells, and flow cytometry were used to detect cell proliferation and apoptosis. The expres-sion level of miR-20a-5p in colon cancer tissues was significantly higher than that in adjacent tissues, and the expression level of BRMS1L was significantly lower than that in adjacent tissues. The expression level of miR-20a-5p was significantly correlated with tumor-node-metastasis (TNM) stage, lymph node metastasis, in-vasion depth, and differentiation degree. The higher the expression level of miR-20a-5p, the more advanced the TNM stage and invasion depth, and the easier it is for lymph nodes to metastasize (p<0.05). Compared with the control and the miR-NC groups, the miR-20a-5p group’s cell proliferation ability, expression of CyclinD1 and B-cell lymphoma-2 (Bcl-2) were significantly increased, while apoptosis ability and caspase-3 protein expression were significantly decreased (p<0.05). The expression of miR-20a-5p in colon cancer tissues and cells in-creased. Overexpression of miR-20a-5p could promote the proliferation of colon cancer cells and inhibit their apoptosis.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47244831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. This study aimed to analyze the sonographic features of metastatic tumorsin patients with thyroid cancer that underwent preoperative ultrasonogra-phy. One hundred and three thyroid cancer patients whose metastases were con-firmed by surgical pathology in The First People’s Hospital of Wenling from January 2020 toDecember 2021 were enrolled. All patients received preoperative ultra-soundexaminations, and the sonographic features were analyzed. Ultrasound ex-aminationshowed 83.50% of cervical lymph node metastasis (CLNM), 24.27% of soft tissueinvasion (STI), 3.88% of distant organ metastasis (DOM), 8.74% of CLNM + STI, 0.97% of CLNM + DOM, and 0.97% of CLNM + STI+DOM. Unilateral CLNM accounted for 72.09%, while bilateral CLNM accounted for 27.91%. The mean longdiameter of metastatic lymph nodes was (1.83±0.63) cm, and the mean short di-ameterwas (1.03±0.42) cm. Metastases to zone II, III, IV, V, VI, and VII accounted for 8.14%, 48.84%, 23.26%, 4.65%, 11.63%, and 3.49%, respectively. The L/T ratio of lymph nodes in 65 cases was lower than 2; 45 of 70 solid metastases exhibited solidhyperechoic, 15 multifocal hyperechoic, seven unifocal hyperechoic, and three diffusely distributed solid hyperechoic images. There were 25 patients with STI thatexperienced invasion of the thyroid capsule, ten patients experienced the invasion of the cervical fatty muscles, two patients had invasion of the trachea, and one pa-tienthad invasion of the thyroid cartilage. Of the four patients with DOM, one had parotidmetastasis, one had submandibular metastasis, one had axillary metastasis, and onehad uterine metastasis. The most common metastatic sites of thyroid can-cer arecervical lymph nodes. However, there were also metastases in the soft tissues anddistant organs. The ultrasonography exhibited typical sonographic features. An adequate familiarity with these sonographic features can aid in detecting suspiciousmetastases in time, which is crucial to the clinical diagnosis, treatment, and prog-nosticassessment.
{"title":"The sonographic features of preoperative ultrasonography of metastatic tumors of thyroid cancer confirmed by surgical pathology","authors":"Lingling Ruan, Qingxin Zhang, Qinghong Qu","doi":"10.54817/ic.v64n2a04","DOIUrl":"https://doi.org/10.54817/ic.v64n2a04","url":null,"abstract":"Abstract. This study aimed to analyze the sonographic features of metastatic tumorsin patients with thyroid cancer that underwent preoperative ultrasonogra-phy. One hundred and three thyroid cancer patients whose metastases were con-firmed by surgical pathology in The First People’s Hospital of Wenling from January 2020 toDecember 2021 were enrolled. All patients received preoperative ultra-soundexaminations, and the sonographic features were analyzed. Ultrasound ex-aminationshowed 83.50% of cervical lymph node metastasis (CLNM), 24.27% of soft tissueinvasion (STI), 3.88% of distant organ metastasis (DOM), 8.74% of CLNM + STI, 0.97% of CLNM + DOM, and 0.97% of CLNM + STI+DOM. Unilateral CLNM accounted for 72.09%, while bilateral CLNM accounted for 27.91%. The mean longdiameter of metastatic lymph nodes was (1.83±0.63) cm, and the mean short di-ameterwas (1.03±0.42) cm. Metastases to zone II, III, IV, V, VI, and VII accounted for 8.14%, 48.84%, 23.26%, 4.65%, 11.63%, and 3.49%, respectively. The L/T ratio of lymph nodes in 65 cases was lower than 2; 45 of 70 solid metastases exhibited solidhyperechoic, 15 multifocal hyperechoic, seven unifocal hyperechoic, and three diffusely distributed solid hyperechoic images. There were 25 patients with STI thatexperienced invasion of the thyroid capsule, ten patients experienced the invasion of the cervical fatty muscles, two patients had invasion of the trachea, and one pa-tienthad invasion of the thyroid cartilage. Of the four patients with DOM, one had parotidmetastasis, one had submandibular metastasis, one had axillary metastasis, and onehad uterine metastasis. The most common metastatic sites of thyroid can-cer arecervical lymph nodes. However, there were also metastases in the soft tissues anddistant organs. The ultrasonography exhibited typical sonographic features. An adequate familiarity with these sonographic features can aid in detecting suspiciousmetastases in time, which is crucial to the clinical diagnosis, treatment, and prog-nosticassessment.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45668907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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