We aimed to evaluate the clinical effects of oxytocin, misopros-tol, controlled-release dinoprostone suppository, and Cook® cervical ripening balloon on early termination of pregnancy in high-risk parturients. Four hun-dred high-risk full-term parturients not in labor who were unsuitable for await-ing delivery and treated from May 2018 to July 2020 were divided into groups I-IV with a random number table (n=100). They received labor induction by oxytocin, misoprostol, controlled-release dinoprostone suppository, and Cook® cervical ripening balloon, respectively. The general data, cervical ripening ef-fect, delivery outcome, delivery time, adverse reactions, and neonatal condi-tions were compared. The time from the beginning of labor induction to labor and duration of the first, third, and total stages of labor were shorter in group II-IV than in group I (p<0.05). The incidence rates of excessive uterine contrac-tion in groups II and III were higher than those of groups I and IV, and the inci-dence rates of fetal distress in groups I-III exceeded that of group IV (p<0.05). The neonatal Apgar scores of groups III and IV were higher than those of groups I and II (p<0.05). Cook® cervical ripening can promote cervical maturation and shorten the labor induction time and stage of labor.
我们旨在评估催产素、米索前列醇、控释地诺前列酮栓和Cook®宫颈成熟球囊对高危产妇提前终止妊娠的临床效果。将2018年5月至2020年7月期间接受治疗的不适合待产的四百名高危足月产产妇用随机数字表法分为I-IV组（n=100）。他们分别接受催产素、米索前列醇、控释地诺前列酮栓和Cook®宫颈成熟球囊引产。比较了一般资料、宫颈成熟效果、分娩结局、分娩时间、不良反应和新生儿情况。II-IV组从引产开始到分娩的时间以及第一产程、第三产程和总产程的持续时间均短于I组（P<0.05）。II 组和 III 组的过度宫缩发生率高于 I 组和 IV 组，I-III 组的胎儿窘迫发生率高于 IV 组（P<0.05）。III 组和 IV 组的新生儿 Apgar 评分高于 I 组和 II 组（P<0.05）。Cook®宫颈成熟术可促进宫颈成熟，缩短引产时间，缩短产程。
{"title":"Clinical effects of Cook® cervical ripening balloon on promoting cervical dilation for early termination of pregnancy in high-risk parturients.","authors":"Xiaorui Han, Junnan Cai, Wei Dong, Ya Li","doi":"10.54817/ic.v65n1a01","DOIUrl":"https://doi.org/10.54817/ic.v65n1a01","url":null,"abstract":"We aimed to evaluate the clinical effects of oxytocin, misopros-tol, controlled-release dinoprostone suppository, and Cook® cervical ripening balloon on early termination of pregnancy in high-risk parturients. Four hun-dred high-risk full-term parturients not in labor who were unsuitable for await-ing delivery and treated from May 2018 to July 2020 were divided into groups I-IV with a random number table (n=100). They received labor induction by oxytocin, misoprostol, controlled-release dinoprostone suppository, and Cook® cervical ripening balloon, respectively. The general data, cervical ripening ef-fect, delivery outcome, delivery time, adverse reactions, and neonatal condi-tions were compared. The time from the beginning of labor induction to labor and duration of the first, third, and total stages of labor were shorter in group II-IV than in group I (p<0.05). The incidence rates of excessive uterine contrac-tion in groups II and III were higher than those of groups I and IV, and the inci-dence rates of fetal distress in groups I-III exceeded that of group IV (p<0.05). The neonatal Apgar scores of groups III and IV were higher than those of groups I and II (p<0.05). Cook® cervical ripening can promote cervical maturation and shorten the labor induction time and stage of labor.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. This research aimed to determine the susceptibility of Aspergil-lus spp. to four antifungal agents using the Etest® method in several clinical samples (respiratory samples, soft tissue, otic tissue, and ocular tissue, among others) from a private health center in Venezuela. Thirty-three strains were evaluated: 11 Aspergillus section Flavi, eight Aspergillus section Fumigati, six Aspergillus section Nigri, four Aspergillus section Terrei, and four Aspergillus spp. A 0.5 McFarland standard suspension of a 5-day culture of each Aspergillusstrain was prepared on Potato Dextrose agar and then inoculated on Sabouraud agar plates with 2% glucose. Voriconazole (VCZ), amphotericin B (AMB), caspo-fungin (CAS), and posaconazole (PCZ) were tested. Minimal inhibitory concen-trations (MIC) in μg/mL were determined after 24 and 48 hours of incubation at 35 °C and th range (R), geometric mean (GM), MIC50, and MIC90 were calcu-lated. The results for the 33 Aspergillus spp. tested after 24 h were the follow-ing: VCZ (R = 0.031- 16; GM = 0.145; MIC50 = 0.125 and MIC90 = 0.5), AMB (R = 0.031-16; GM = 0.644; MIC50 = 0.5 and MIC90 = 8), CAS (R = 0.031-16; GM = 0.1076; MIC50 = 0.063 and MIC90 = 1), PCZ (R =0.031 - 0.5; GM = 0.0755; MIC50 = 0.063 and MIC90 = 0.25). This investigation allowed assessing the an-tifungal susceptibility profiles of Aspergillus spp. isolated from clinical samples by the Etest® method, which is practical, reproducible and easy to perform in microbiology laboratories.
{"title":"Antifungal susceptibility of Aspergillusgenus determined by the Etest® method: eleven years of experience at the Instituto Médico La Floresta. Caracas, Venezuela.","authors":"Xiomara Moreno Calderón, Carolina Macero Estévez, Débora Oliveira Oliveira","doi":"10.54817/ic.v64n4a04","DOIUrl":"https://doi.org/10.54817/ic.v64n4a04","url":null,"abstract":"Abstract. This research aimed to determine the susceptibility of Aspergil-lus spp. to four antifungal agents using the Etest® method in several clinical samples (respiratory samples, soft tissue, otic tissue, and ocular tissue, among others) from a private health center in Venezuela. Thirty-three strains were evaluated: 11 Aspergillus section Flavi, eight Aspergillus section Fumigati, six Aspergillus section Nigri, four Aspergillus section Terrei, and four Aspergillus spp. A 0.5 McFarland standard suspension of a 5-day culture of each Aspergillusstrain was prepared on Potato Dextrose agar and then inoculated on Sabouraud agar plates with 2% glucose. Voriconazole (VCZ), amphotericin B (AMB), caspo-fungin (CAS), and posaconazole (PCZ) were tested. Minimal inhibitory concen-trations (MIC) in μg/mL were determined after 24 and 48 hours of incubation at 35 °C and th range (R), geometric mean (GM), MIC50, and MIC90 were calcu-lated. The results for the 33 Aspergillus spp. tested after 24 h were the follow-ing: VCZ (R = 0.031- 16; GM = 0.145; MIC50 = 0.125 and MIC90 = 0.5), AMB (R = 0.031-16; GM = 0.644; MIC50 = 0.5 and MIC90 = 8), CAS (R = 0.031-16; GM = 0.1076; MIC50 = 0.063 and MIC90 = 1), PCZ (R =0.031 - 0.5; GM = 0.0755; MIC50 = 0.063 and MIC90 = 0.25). This investigation allowed assessing the an-tifungal susceptibility profiles of Aspergillus spp. isolated from clinical samples by the Etest® method, which is practical, reproducible and easy to perform in microbiology laboratories.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139247352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adolescent suicide has increased in the Americas. In Chile, at-tempted suicide in adolescents is a public health problem that needs an urgent solution. A quantitative, comparative and retrospective study was conducted to provide the hospital with intervention strategies and articulation with the territorial mental health network. The sociodemographic and clinical aspects of 96 records of adolescents hospitalized for attempted suicide between Janu-ary 2017 and December 2018 were reviewed, compared, and related. Two study groups were formed: Group I, 14 adolescents previously hospitalized in the Intensive Treatment Unit and Group II, 82 adolescents who did not require this unit. Gender, age, education, history of sexual abuse, diagnoses of mental disorders, co-morbidities, previous mental health consultations, and caregivers with mental disorders were studied. Descriptive statistics and odds ratios were used. In group I, with 92% male adolescents, severe depression was observed in 57% of cases, and 100% of its members suffered sexual abuse; in group II, with 91% female adolescents, 79% moderate depression was found, 85.3% ab-normal personality development and 70% of its members suffered sexual abuse. Sexual abuse explained the highest percentage of the variance (29%). It is con-cluded that the hospital must consider the distinctive characteristics of the study groups and coordinate with the territorial network of mental health the continuity of care in its interventions.
{"title":"Caracterización de la gravedad del intento de suicidio en adolescentes hospitalizados en un hospital público de Chile.","authors":"L. Barriga","doi":"10.54817/ic.v64n4a02","DOIUrl":"https://doi.org/10.54817/ic.v64n4a02","url":null,"abstract":"Adolescent suicide has increased in the Americas. In Chile, at-tempted suicide in adolescents is a public health problem that needs an urgent solution. A quantitative, comparative and retrospective study was conducted to provide the hospital with intervention strategies and articulation with the territorial mental health network. The sociodemographic and clinical aspects of 96 records of adolescents hospitalized for attempted suicide between Janu-ary 2017 and December 2018 were reviewed, compared, and related. Two study groups were formed: Group I, 14 adolescents previously hospitalized in the Intensive Treatment Unit and Group II, 82 adolescents who did not require this unit. Gender, age, education, history of sexual abuse, diagnoses of mental disorders, co-morbidities, previous mental health consultations, and caregivers with mental disorders were studied. Descriptive statistics and odds ratios were used. In group I, with 92% male adolescents, severe depression was observed in 57% of cases, and 100% of its members suffered sexual abuse; in group II, with 91% female adolescents, 79% moderate depression was found, 85.3% ab-normal personality development and 70% of its members suffered sexual abuse. Sexual abuse explained the highest percentage of the variance (29%). It is con-cluded that the hospital must consider the distinctive characteristics of the study groups and coordinate with the territorial network of mental health the continuity of care in its interventions.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"649 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139247842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim was to explore the influencing factors of post-trans-plantation diabetes mellitus (PTDM) in kidney transplant recipients and to es-tablish a risk prediction model. A retrospective analysis was performed on the clinical data of 408 patients subjected to kidney transplantation from May 2015 to March 2022. With the simple random sampling method, they were divided into a training set (n=306) and a test set (n=102) at a ratio of 3:1. According to the occurrence of PTDM, the training set was further classified into PTDM and non-PTDM groups. The influencing factors of PTDM were identified by least absolute shrinkage and selection operator and multivariate logistic regression analysis. A nomogram prediction model was constructed and validated. Non-PT-DM and PTDM groups had significantly different preoperative body mass index (BMI), family history of diabetes mellitus, 2-h preoperative and postprandial blood glucose, 2-hpreoperative and postprandial peptide index, postoperative hypomagnesemia, whole blood concentration of tacrolimus, triacylglycerol, glycated albumin and fasting blood glucose (P<0.05). BMI, family history of diabetes mellitus, 2-h preoperative and postprandial blood glucose, and post-operative whole blood tacrolimus concentration were independent risk factors for PTDM. In contrast, the 2-h preoperative and postprandial peptide index was an independent protective factor (P<0.05). The incidence of PTDM in patients receiving kidney transplantation correlates with the family history of diabetes mellitus, preoperative BMI, 2-h postprandial blood glucose, 2-h postprandial peptide index, and postoperative whole blood tacrolimus concentration.
{"title":"Influencing factors of post-transplantation diabetes mellitus in kidney transplant recipients and establishment of a risk prediction model.","authors":"Yuan Dong","doi":"10.54817/ic.v64n4a03","DOIUrl":"https://doi.org/10.54817/ic.v64n4a03","url":null,"abstract":"The aim was to explore the influencing factors of post-trans-plantation diabetes mellitus (PTDM) in kidney transplant recipients and to es-tablish a risk prediction model. A retrospective analysis was performed on the clinical data of 408 patients subjected to kidney transplantation from May 2015 to March 2022. With the simple random sampling method, they were divided into a training set (n=306) and a test set (n=102) at a ratio of 3:1. According to the occurrence of PTDM, the training set was further classified into PTDM and non-PTDM groups. The influencing factors of PTDM were identified by least absolute shrinkage and selection operator and multivariate logistic regression analysis. A nomogram prediction model was constructed and validated. Non-PT-DM and PTDM groups had significantly different preoperative body mass index (BMI), family history of diabetes mellitus, 2-h preoperative and postprandial blood glucose, 2-hpreoperative and postprandial peptide index, postoperative hypomagnesemia, whole blood concentration of tacrolimus, triacylglycerol, glycated albumin and fasting blood glucose (P<0.05). BMI, family history of diabetes mellitus, 2-h preoperative and postprandial blood glucose, and post-operative whole blood tacrolimus concentration were independent risk factors for PTDM. In contrast, the 2-h preoperative and postprandial peptide index was an independent protective factor (P<0.05). The incidence of PTDM in patients receiving kidney transplantation correlates with the family history of diabetes mellitus, preoperative BMI, 2-h postprandial blood glucose, 2-h postprandial peptide index, and postoperative whole blood tacrolimus concentration.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bixiang Zheng, Xiaobin Luo, Changdong Wang, Rendong Zheng, Xiaofeng Yang
The aim was to investigate the effect of different peritoneal tear closure methods on the operative outcomes and prognosis of patients undergo-ing laparoscopic inguinal hernia repair (LIHR). Ninety patients who underwent LIHR in our hospital from August 2019 to December 2020 and had peritoneal tears during the operation were selected, and the patients were divided into a control group (CG) and the observation group (OG) according to different treatment plans, with 45 cases in each group. Patients in the CG were treated with absorbable sutures to repair the peritoneal tears, while patients in the OG were treated with bipolar coagulation to close and repair the peritoneal tears. The surgical conditions, postoperative pain scores, quality of life scores, com-plications, and recurrence were compared between the CG and OG groups. The operation time and hospital stay in the OG were shorter than those in the OG (p<0.05). The pain scores in the OG at 24 hours after operation were lower than those in the CG (p<0.05), and the pain scores of the two groups were not significantly different at two hours and 12 hours (p>0.05). Postoperative complications were not significantly different between the groups (p>0.05). The scores of material life, physical, social, and psychological function in the OG were higher than in the CG (p<0.05). There were no recurrences in the two groups during the 1-year follow-up. Closing repair of peritoneal rupture with bipolar coagulation reduces the operation time of patients with peritoneal rup-ture during TEP (total extraperitoneal hernioplasty) operations, reduces pain, and improves their quality of life. The treatment outcome is safe, effective, and has an excellent clinical application effect.
{"title":"Influence of different peritoneal incision closure methods on the operative outcomes and prognosis of patients undergoing laparoscopic inguinal hernia repair.","authors":"Bixiang Zheng, Xiaobin Luo, Changdong Wang, Rendong Zheng, Xiaofeng Yang","doi":"10.54817/ic.v64n4a06","DOIUrl":"https://doi.org/10.54817/ic.v64n4a06","url":null,"abstract":"The aim was to investigate the effect of different peritoneal tear closure methods on the operative outcomes and prognosis of patients undergo-ing laparoscopic inguinal hernia repair (LIHR). Ninety patients who underwent LIHR in our hospital from August 2019 to December 2020 and had peritoneal tears during the operation were selected, and the patients were divided into a control group (CG) and the observation group (OG) according to different treatment plans, with 45 cases in each group. Patients in the CG were treated with absorbable sutures to repair the peritoneal tears, while patients in the OG were treated with bipolar coagulation to close and repair the peritoneal tears. The surgical conditions, postoperative pain scores, quality of life scores, com-plications, and recurrence were compared between the CG and OG groups. The operation time and hospital stay in the OG were shorter than those in the OG (p<0.05). The pain scores in the OG at 24 hours after operation were lower than those in the CG (p<0.05), and the pain scores of the two groups were not significantly different at two hours and 12 hours (p>0.05). Postoperative complications were not significantly different between the groups (p>0.05). The scores of material life, physical, social, and psychological function in the OG were higher than in the CG (p<0.05). There were no recurrences in the two groups during the 1-year follow-up. Closing repair of peritoneal rupture with bipolar coagulation reduces the operation time of patients with peritoneal rup-ture during TEP (total extraperitoneal hernioplasty) operations, reduces pain, and improves their quality of life. The treatment outcome is safe, effective, and has an excellent clinical application effect.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to determine the frequency of multidrug-resis-tant (MDR) and extensively drug-resistant (XDR) uropathogens isolated from adult patients in Barinas City, Venezuela, in 2022. Of 1019 urine cultures from patients who attended the Barinas Clinical Microbiological Laboratory, 337 (33.07%) were selected according to inclusion criteria. The microbiological processing of urine was carried out through conventional methods. Antimicro-bial susceptibility tests and extended-spectrum beta-lactamase determination were performed using standardized phenotypic methods. The classification of MDR and XDR strains was conducted according to international criteria. Data were processed through descriptive statistics and frequency analysis. Of 337 urine cultures analyzed, 70.92% corresponded to female patients, and the age groups between 31 and 60 years were the most frequent. Of the uropathogens identified, 93.17% were represented by Enterobacterales, where Escherichia coli stood out with 87.54%, followed by Pseudomonasaeruginosa (2.67%) and Enterococcus faecalis (1.48%). Of the E. coli strains, 87.12% presented resis-tance to at least one antibiotic, with 46.78% MDR and 5.42% XDR. In general, more than half of the uropathogens identified were distributed either as MDR or XDR phenotypes. The results show the need to develop local research to im-prove empirical and targeted therapies in urinary tract infections, in addition to awareness actions for the rational use of antibiotics and epidemiological surveillance of multiresistant strains circulating in the region.
{"title":"Uropatógenos multirresistentes y con resistencia extendida a los antimicrobianos aislados en pacientes adultos de la comunidad de Barinas, Venezuela.","authors":"Poema Salazar, María Araque","doi":"10.54817/ic.v64n4a09","DOIUrl":"https://doi.org/10.54817/ic.v64n4a09","url":null,"abstract":"This study aimed to determine the frequency of multidrug-resis-tant (MDR) and extensively drug-resistant (XDR) uropathogens isolated from adult patients in Barinas City, Venezuela, in 2022. Of 1019 urine cultures from patients who attended the Barinas Clinical Microbiological Laboratory, 337 (33.07%) were selected according to inclusion criteria. The microbiological processing of urine was carried out through conventional methods. Antimicro-bial susceptibility tests and extended-spectrum beta-lactamase determination were performed using standardized phenotypic methods. The classification of MDR and XDR strains was conducted according to international criteria. Data were processed through descriptive statistics and frequency analysis. Of 337 urine cultures analyzed, 70.92% corresponded to female patients, and the age groups between 31 and 60 years were the most frequent. Of the uropathogens identified, 93.17% were represented by Enterobacterales, where Escherichia coli stood out with 87.54%, followed by Pseudomonasaeruginosa (2.67%) and Enterococcus faecalis (1.48%). Of the E. coli strains, 87.12% presented resis-tance to at least one antibiotic, with 46.78% MDR and 5.42% XDR. In general, more than half of the uropathogens identified were distributed either as MDR or XDR phenotypes. The results show the need to develop local research to im-prove empirical and targeted therapies in urinary tract infections, in addition to awareness actions for the rational use of antibiotics and epidemiological surveillance of multiresistant strains circulating in the region.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Martínez-Méndez, M. Bravo-Acosta, Neomar Semprún-Hernández
It is estimated that 300 million people have some fungal infec-tion, and 1.5 million die annually because of it, similar to the mortality from tuberculosis and three times more than malaria. These numbers may be higher since mycoses are not mandatory reporting. With the lessons learned during the SARS-CoV-19 pandemic, the 2022 outbreak of Monkeypox, the resistance to antibacterial, and the recognition by the WHO that mycoses receive very little attention and resources, added to the fact that available antifungals have significant adverse effects, poor oral bioavailability and growing resistance, it is imperative to develop new antifungals with better pharmacokinetics and phar-macodynamic characteristics, a broad spectrum at affordable costs and world-wide supply. It is what we want. A huge challenge.
{"title":"Antifúngicos: lo que tenemos, lo que tendremos, lo que queremos.","authors":"D. Martínez-Méndez, M. Bravo-Acosta, Neomar Semprún-Hernández","doi":"10.54817/ic.v64n4a11","DOIUrl":"https://doi.org/10.54817/ic.v64n4a11","url":null,"abstract":"It is estimated that 300 million people have some fungal infec-tion, and 1.5 million die annually because of it, similar to the mortality from tuberculosis and three times more than malaria. These numbers may be higher since mycoses are not mandatory reporting. With the lessons learned during the SARS-CoV-19 pandemic, the 2022 outbreak of Monkeypox, the resistance to antibacterial, and the recognition by the WHO that mycoses receive very little attention and resources, added to the fact that available antifungals have significant adverse effects, poor oral bioavailability and growing resistance, it is imperative to develop new antifungals with better pharmacokinetics and phar-macodynamic characteristics, a broad spectrum at affordable costs and world-wide supply. It is what we want. A huge challenge.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jesús A. Mosquera-Sulbaran, A. Pedreáñez, Yenddy Carrero, Catherina Peña
The receptor for advanced glycation end products (RAGE) is a transmembrane protein involved in the induction of inflammatory processes and oxidative stress after interacting with its ligands on the cell surface. Lo-calization on the cell surface is necessary for interaction with the ligands. This study aimed to determine the expression of RAGE in different parts of the nor-mal rat brain and cerebellum using the immunofluorescence technique. Sev-eralcerebral cortex layers (molecular/granular layers: M/GL; pyramidal layer: PL) and the hypothalamus were analyzed, as well as the molecular layer (CML) and the granular layer (CGL) of the cerebellum. Cells with RAGE-positive nu-clei were generally observed in the brain’s cerebral cortex and cerebellum. In the M/GL, cells with different degrees of positivity in the nucleus and cyto-plasm accompanied by RAGE-positive material in the adjacent extracellular space were observed, and RAGE-positive material in the neuropile. Pyramidal neurons presenting various degrees of nuclear RAGE-positive material budding and cells with different degrees of nuclear and cytoplasmic positivity were ob-served in PL. The hypothalamus showed a high number of cells with RAGE-positive granules adjacent to the nucleus and in the cytoplasm; nuclei remained negative. Many positive nuclei were observed in CML; they were scarce in CGL. These data suggest the storage of RAGE at the nuclear and cytoplasmic levels in healthy rats and hypothesize the possible translocation of this molecule to the cell surface in pathological conditions.
{"title":"Nuclear and cytoplasmic expressions of the receptor for advanced glycation end products (RAGE) in the rat central nervous system.","authors":"Jesús A. Mosquera-Sulbaran, A. Pedreáñez, Yenddy Carrero, Catherina Peña","doi":"10.54817/ic.v64n4a07","DOIUrl":"https://doi.org/10.54817/ic.v64n4a07","url":null,"abstract":"The receptor for advanced glycation end products (RAGE) is a transmembrane protein involved in the induction of inflammatory processes and oxidative stress after interacting with its ligands on the cell surface. Lo-calization on the cell surface is necessary for interaction with the ligands. This study aimed to determine the expression of RAGE in different parts of the nor-mal rat brain and cerebellum using the immunofluorescence technique. Sev-eralcerebral cortex layers (molecular/granular layers: M/GL; pyramidal layer: PL) and the hypothalamus were analyzed, as well as the molecular layer (CML) and the granular layer (CGL) of the cerebellum. Cells with RAGE-positive nu-clei were generally observed in the brain’s cerebral cortex and cerebellum. In the M/GL, cells with different degrees of positivity in the nucleus and cyto-plasm accompanied by RAGE-positive material in the adjacent extracellular space were observed, and RAGE-positive material in the neuropile. Pyramidal neurons presenting various degrees of nuclear RAGE-positive material budding and cells with different degrees of nuclear and cytoplasmic positivity were ob-served in PL. The hypothalamus showed a high number of cells with RAGE-positive granules adjacent to the nucleus and in the cytoplasm; nuclei remained negative. Many positive nuclei were observed in CML; they were scarce in CGL. These data suggest the storage of RAGE at the nuclear and cytoplasmic levels in healthy rats and hypothesize the possible translocation of this molecule to the cell surface in pathological conditions.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"51 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SU5416 is a small molecule vascular endothelial growth factor (VEGF) receptor signal transduction inhibitor, which can block the VEGF re-ceptor autophosphorylation and inhibit receptor tyrosine kinase signal trans-duction, thereby reducing VEGF activity. However, there are few reports about the correlation of SU5416 to the occurrence and angiogenesis in endometrio-sis. In this study, we observed the effects of VEGF receptor inhibitor SU5416 on angiogenesis in endometriosis in rats. Thirty femalespecific-pathogen-free Sprague-Dawley rats were randomly divided into sham operation group (SOG), model group (MG), and SU5416 group (n=10 for each group). In the SOG, only the uterus was cut and sutured, and endometriosis models were established in the MG and SU5416 group by autologous transplantation. The SU5416 group was injected with 15 mg/kg SU5416 intraperitoneally, and the SOG and MG were intraperitoneally injected with an equal volume of normal saline for 6 weeks. The volume of ectopic lesions was lower in the SU5416 group at 42 d postoperatively thanin the MG (p<0.05). The proportion of CD31-positive cells in the endometrial tissue of the SU5416 group was lower than that of the MG (p<0.05); angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), laminin-5γ2 (LN-5γ2) and phosphorylation of ERK (P-ERK), VEGF, matrix metalloproteinase (MMP)-2, and MMP-9 protein expressions were lower in the SU5416 groupthan in the MG (p<0.05).VEGFreceptor inhibitor SU5416 can inhibit endometrio-sis angiogenesis and reduce inflammatory response in rats, and its mechanism of action may be related to the down-regulation of the ERK-VEGF/MMP-9 path-way expression.
{"title":"Effects of SU5416 on angiogenesis and the ERK-VEGF/MMP-9 pathway in rat endometriosis.","authors":"Danyang Zhao, Qiufang Bao, Lihong Chen, Lie Zheng","doi":"10.54817/ic.v64n4a05","DOIUrl":"https://doi.org/10.54817/ic.v64n4a05","url":null,"abstract":"SU5416 is a small molecule vascular endothelial growth factor (VEGF) receptor signal transduction inhibitor, which can block the VEGF re-ceptor autophosphorylation and inhibit receptor tyrosine kinase signal trans-duction, thereby reducing VEGF activity. However, there are few reports about the correlation of SU5416 to the occurrence and angiogenesis in endometrio-sis. In this study, we observed the effects of VEGF receptor inhibitor SU5416 on angiogenesis in endometriosis in rats. Thirty femalespecific-pathogen-free Sprague-Dawley rats were randomly divided into sham operation group (SOG), model group (MG), and SU5416 group (n=10 for each group). In the SOG, only the uterus was cut and sutured, and endometriosis models were established in the MG and SU5416 group by autologous transplantation. The SU5416 group was injected with 15 mg/kg SU5416 intraperitoneally, and the SOG and MG were intraperitoneally injected with an equal volume of normal saline for 6 weeks. The volume of ectopic lesions was lower in the SU5416 group at 42 d postoperatively thanin the MG (p<0.05). The proportion of CD31-positive cells in the endometrial tissue of the SU5416 group was lower than that of the MG (p<0.05); angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), laminin-5γ2 (LN-5γ2) and phosphorylation of ERK (P-ERK), VEGF, matrix metalloproteinase (MMP)-2, and MMP-9 protein expressions were lower in the SU5416 groupthan in the MG (p<0.05).VEGFreceptor inhibitor SU5416 can inhibit endometrio-sis angiogenesis and reduce inflammatory response in rats, and its mechanism of action may be related to the down-regulation of the ERK-VEGF/MMP-9 path-way expression.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gulsah Gulsah Aynaoglu Yildiz, Omer Erkan Yapca, K. Dinç, Cebrail Gursul, Betul Gundogdu, Mehmet Aktas, Z. Suleyman, S. Bulut, H. Suleyman
Abstract. Many types of stress, including psychological stress, nega-tively affect reproductive health. This study aimed to investigate the ef-fects of sertraline (a selective serotonin reuptake inhibitor), cerebrolysin (neuroprotective/neurotrophic), and a combination of both against stress-induced ovarian damage, infertility and pregnancy delay in female rats. The rats were divided into five groups (n=14/each group) as healthy (HG), stress control (StC), stress+sertraline (SS), stress+cerebrolysin (SC), and stress+sertraline+cerebrolysin (SSC). To induce stress, animals (except the HG) were kept in a supine position with their forelimbs and hindlimbs (FIM) tied for one hour. Then, sertraline (20mg/kg) was given orally to the SS. Cerebrolysin (2.5ml/kg) was injected into the SC subcutaneously. Sertraline+cerebrolysin was administered to SSC with the same methods and doses. FIM and drug administration continued for 30 days. Six rats from each group were euthanized with high-dose anesthesia, right and left ovarian tissues were removed, and tissues were examined biochemically and histopathologi-cally. The remaining rats were taken for breeding. Exposure to stress in rats caused an increase in malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL -1β), and interleukin-6 (IL -6) levels and a decrease in total glutathione (tGSH). Stress was related to histopathological damage, infertility, and delayed birth. The sertraline and cerebrolysin combination was the most effective in preventing these changes, with sertraline and cerebroly-sin alone in second and third places, respectively. Regarding efficacy, selective serotonin reuptake inhibitors (SSRIs) and related drugs may be beneficial in treating stress-related ovarian damage, infertility, and delay in pregnancy.
摘要包括心理压力在内的多种压力会对生殖健康产生负面影响。本研究旨在探讨舍曲林（一种选择性 5-羟色胺再摄取抑制剂）、脑复康（神经保护/神经营养剂）以及二者的组合对应激诱导的雌性大鼠卵巢损伤、不孕和妊娠延迟的影响。大鼠分为五组（n=14/每组），分别为健康组（HG）、应激对照组（StC）、应激+舍曲林组（SS）、应激+脑磷脂组（SC）和应激+舍曲林+脑磷脂组（SSC）。为了诱导应激，将动物（HG 除外）的前肢和后肢（FIM）捆绑后保持仰卧位一小时。然后，给 SS 口服舍曲林（20 毫克/千克）。向SC皮下注射脑啡肽（2.5毫升/千克）。用同样的方法和剂量给SSC注射舍曲林+脑溶素。FIM和给药持续30天。每组六只大鼠经大剂量麻醉后安乐死，取出左右卵巢组织，进行生化和组织病理学检查。其余大鼠用于繁殖。大鼠暴露于应激会导致丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平升高，总谷胱甘肽（tGSH）水平降低。压力与组织病理学损伤、不孕症和晚产有关。舍曲林和脑复康联合用药在预防这些变化方面最为有效，舍曲林和脑复康单独用药分别排在第二和第三位。就疗效而言，选择性血清素再摄取抑制剂（SSRIs）及相关药物可能有助于治疗与压力有关的卵巢损伤、不孕症和妊娠延迟。
{"title":"Stress-associated ovarian damage, infertility, and delay in achieving pregnancy and treatment options.","authors":"Gulsah Gulsah Aynaoglu Yildiz, Omer Erkan Yapca, K. Dinç, Cebrail Gursul, Betul Gundogdu, Mehmet Aktas, Z. Suleyman, S. Bulut, H. Suleyman","doi":"10.54817/ic.v64n4a08","DOIUrl":"https://doi.org/10.54817/ic.v64n4a08","url":null,"abstract":"Abstract. Many types of stress, including psychological stress, nega-tively affect reproductive health. This study aimed to investigate the ef-fects of sertraline (a selective serotonin reuptake inhibitor), cerebrolysin (neuroprotective/neurotrophic), and a combination of both against stress-induced ovarian damage, infertility and pregnancy delay in female rats. The rats were divided into five groups (n=14/each group) as healthy (HG), stress control (StC), stress+sertraline (SS), stress+cerebrolysin (SC), and stress+sertraline+cerebrolysin (SSC). To induce stress, animals (except the HG) were kept in a supine position with their forelimbs and hindlimbs (FIM) tied for one hour. Then, sertraline (20mg/kg) was given orally to the SS. Cerebrolysin (2.5ml/kg) was injected into the SC subcutaneously. Sertraline+cerebrolysin was administered to SSC with the same methods and doses. FIM and drug administration continued for 30 days. Six rats from each group were euthanized with high-dose anesthesia, right and left ovarian tissues were removed, and tissues were examined biochemically and histopathologi-cally. The remaining rats were taken for breeding. Exposure to stress in rats caused an increase in malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL -1β), and interleukin-6 (IL -6) levels and a decrease in total glutathione (tGSH). Stress was related to histopathological damage, infertility, and delayed birth. The sertraline and cerebrolysin combination was the most effective in preventing these changes, with sertraline and cerebroly-sin alone in second and third places, respectively. Regarding efficacy, selective serotonin reuptake inhibitors (SSRIs) and related drugs may be beneficial in treating stress-related ovarian damage, infertility, and delay in pregnancy.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"140 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139246994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：