Gulsah Gulsah Aynaoglu Yildiz, Omer Erkan Yapca, K. Dinç, Cebrail Gursul, Betul Gundogdu, Mehmet Aktas, Z. Suleyman, S. Bulut, H. Suleyman
Abstract. Many types of stress, including psychological stress, nega-tively affect reproductive health. This study aimed to investigate the ef-fects of sertraline (a selective serotonin reuptake inhibitor), cerebrolysin (neuroprotective/neurotrophic), and a combination of both against stress-induced ovarian damage, infertility and pregnancy delay in female rats. The rats were divided into five groups (n=14/each group) as healthy (HG), stress control (StC), stress+sertraline (SS), stress+cerebrolysin (SC), and stress+sertraline+cerebrolysin (SSC). To induce stress, animals (except the HG) were kept in a supine position with their forelimbs and hindlimbs (FIM) tied for one hour. Then, sertraline (20mg/kg) was given orally to the SS. Cerebrolysin (2.5ml/kg) was injected into the SC subcutaneously. Sertraline+cerebrolysin was administered to SSC with the same methods and doses. FIM and drug administration continued for 30 days. Six rats from each group were euthanized with high-dose anesthesia, right and left ovarian tissues were removed, and tissues were examined biochemically and histopathologi-cally. The remaining rats were taken for breeding. Exposure to stress in rats caused an increase in malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL -1β), and interleukin-6 (IL -6) levels and a decrease in total glutathione (tGSH). Stress was related to histopathological damage, infertility, and delayed birth. The sertraline and cerebrolysin combination was the most effective in preventing these changes, with sertraline and cerebroly-sin alone in second and third places, respectively. Regarding efficacy, selective serotonin reuptake inhibitors (SSRIs) and related drugs may be beneficial in treating stress-related ovarian damage, infertility, and delay in pregnancy.
摘要包括心理压力在内的多种压力会对生殖健康产生负面影响。本研究旨在探讨舍曲林（一种选择性 5-羟色胺再摄取抑制剂）、脑复康（神经保护/神经营养剂）以及二者的组合对应激诱导的雌性大鼠卵巢损伤、不孕和妊娠延迟的影响。大鼠分为五组（n=14/每组），分别为健康组（HG）、应激对照组（StC）、应激+舍曲林组（SS）、应激+脑磷脂组（SC）和应激+舍曲林+脑磷脂组（SSC）。为了诱导应激，将动物（HG 除外）的前肢和后肢（FIM）捆绑后保持仰卧位一小时。然后，给 SS 口服舍曲林（20 毫克/千克）。向SC皮下注射脑啡肽（2.5毫升/千克）。用同样的方法和剂量给SSC注射舍曲林+脑溶素。FIM和给药持续30天。每组六只大鼠经大剂量麻醉后安乐死，取出左右卵巢组织，进行生化和组织病理学检查。其余大鼠用于繁殖。大鼠暴露于应激会导致丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平升高，总谷胱甘肽（tGSH）水平降低。压力与组织病理学损伤、不孕症和晚产有关。舍曲林和脑复康联合用药在预防这些变化方面最为有效，舍曲林和脑复康单独用药分别排在第二和第三位。就疗效而言，选择性血清素再摄取抑制剂（SSRIs）及相关药物可能有助于治疗与压力有关的卵巢损伤、不孕症和妊娠延迟。
{"title":"Stress-associated ovarian damage, infertility, and delay in achieving pregnancy and treatment options.","authors":"Gulsah Gulsah Aynaoglu Yildiz, Omer Erkan Yapca, K. Dinç, Cebrail Gursul, Betul Gundogdu, Mehmet Aktas, Z. Suleyman, S. Bulut, H. Suleyman","doi":"10.54817/ic.v64n4a08","DOIUrl":"https://doi.org/10.54817/ic.v64n4a08","url":null,"abstract":"Abstract. Many types of stress, including psychological stress, nega-tively affect reproductive health. This study aimed to investigate the ef-fects of sertraline (a selective serotonin reuptake inhibitor), cerebrolysin (neuroprotective/neurotrophic), and a combination of both against stress-induced ovarian damage, infertility and pregnancy delay in female rats. The rats were divided into five groups (n=14/each group) as healthy (HG), stress control (StC), stress+sertraline (SS), stress+cerebrolysin (SC), and stress+sertraline+cerebrolysin (SSC). To induce stress, animals (except the HG) were kept in a supine position with their forelimbs and hindlimbs (FIM) tied for one hour. Then, sertraline (20mg/kg) was given orally to the SS. Cerebrolysin (2.5ml/kg) was injected into the SC subcutaneously. Sertraline+cerebrolysin was administered to SSC with the same methods and doses. FIM and drug administration continued for 30 days. Six rats from each group were euthanized with high-dose anesthesia, right and left ovarian tissues were removed, and tissues were examined biochemically and histopathologi-cally. The remaining rats were taken for breeding. Exposure to stress in rats caused an increase in malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL -1β), and interleukin-6 (IL -6) levels and a decrease in total glutathione (tGSH). Stress was related to histopathological damage, infertility, and delayed birth. The sertraline and cerebrolysin combination was the most effective in preventing these changes, with sertraline and cerebroly-sin alone in second and third places, respectively. Regarding efficacy, selective serotonin reuptake inhibitors (SSRIs) and related drugs may be beneficial in treating stress-related ovarian damage, infertility, and delay in pregnancy.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"140 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139246994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cervical cancer is the fourth most common malignant tumor in women. Many studies have confirmed that early childbirth, prolificacy, HPV infection, and smoking are some risk factors. This article explored the effects of exposing human cervical cancer HeLa cells to different focused ultrasound intensities in vitro. The study employed three groups of cells: 1- a high-intensity treated group, 2- a low-intensity treated group, and 3- a control group. Results showed that after 12 hours of focused ultrasound treatment, the growth inhibi-tion rate of the low-intensity group was 55.6% higher than that of the control group, and the growth inhibition rate of the high-intensity group was 41.2% higher than that of the low-intensity group. Therefore, focused ultrasound had a specific inhibitory effect on the growth of HeLa cells, and the higher the intensity of focused ultrasound, the higher the inhibition rate on cancer cells. In addition, the Cycle Threshold (Ct) values of the three groups of cells before treatment were the same, but the Ct values after treatment had changed. The Ct value of the low-intensity group was 18.1% lower than that of the control group, and the Ct value of the high-intensity group was lower than that of the low-intensity group by 27.8%, showing that focused ultrasound can effectively reduce the activity of HeLa cells in vitro.
宫颈癌是女性第四大常见恶性肿瘤。许多研究证实，早产、多产、人乳头瘤病毒感染和吸烟是一些危险因素。本文探讨了在体外将人类宫颈癌 HeLa 细胞暴露于不同聚焦超声强度的影响。研究采用了三组细胞：1-高强度处理组，2-低强度处理组，3-对照组。结果显示，聚焦超声处理 12 小时后，低强度组的生长抑制率比对照组高 55.6%，高强度组的生长抑制率比低强度组高 41.2%。因此，聚焦超声对 HeLa 细胞的生长有特异性抑制作用，聚焦超声强度越高，对癌细胞的抑制率越高。此外，三组细胞治疗前的周期阈值（Ct）相同，但治疗后的 Ct 值发生了变化。低强度组的 Ct 值比对照组低 18.1%，高强度组的 Ct 值比低强度组低 27.8%，这表明聚焦超声能有效降低 HeLa 细胞在体外的活性。
{"title":"Effects of focused ultrasound on human cervical cancer HeLa cells in vitro.","authors":"Yanbin Liu, Qun Zhao, Panpan Liu, Yanbin Li, Li’an Yi, Haiping Yan","doi":"10.54817/ic.v64n4a01","DOIUrl":"https://doi.org/10.54817/ic.v64n4a01","url":null,"abstract":"Cervical cancer is the fourth most common malignant tumor in women. Many studies have confirmed that early childbirth, prolificacy, HPV infection, and smoking are some risk factors. This article explored the effects of exposing human cervical cancer HeLa cells to different focused ultrasound intensities in vitro. The study employed three groups of cells: 1- a high-intensity treated group, 2- a low-intensity treated group, and 3- a control group. Results showed that after 12 hours of focused ultrasound treatment, the growth inhibi-tion rate of the low-intensity group was 55.6% higher than that of the control group, and the growth inhibition rate of the high-intensity group was 41.2% higher than that of the low-intensity group. Therefore, focused ultrasound had a specific inhibitory effect on the growth of HeLa cells, and the higher the intensity of focused ultrasound, the higher the inhibition rate on cancer cells. In addition, the Cycle Threshold (Ct) values of the three groups of cells before treatment were the same, but the Ct values after treatment had changed. The Ct value of the low-intensity group was 18.1% lower than that of the control group, and the Ct value of the high-intensity group was lower than that of the low-intensity group by 27.8%, showing that focused ultrasound can effectively reduce the activity of HeLa cells in vitro.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"197 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The spiked helmet sign (SHS) is a type of ST-segment elevation associated with critical cardiac disease and a high risk of death. We report a case of SHS caused by an ECG artifact. A 60-year-old male patient presented to the clinic after suffering an electric shock. The initial 12-lead routine elec-trocardiogram showed an SHS. The patient received appropriate intravenous fluid replacement therapy, and after 30 minutes, the ST-T changes of the 12-lead electrocardiogram were all restored to normal. The patient was discharged after a 24-hour observation period in the emergency room.Recent studies have pointed out that there may be two different types of SHS. One is the mechanical factor, and the other is the significant prolongation of the QT interval. The two types have different clinical significance. In our report, the radial artery of the patient’s right wrist pulsed strongly, and after the occurrence of SHS, the SHS disappeared after adjusting the contact position of the electrode in his right arm. This SHS caused by mechanical traction was an ECG artifact.Although the SHS may be an essential indicator of critical illness, there are mechanical factors that lead to the appearance of ECG artifacts. Therefore, in clinical work, obtaining a complete medical history and primary conditions of the patient at the time of ECG sampling is necessary to help the diagnosis and thus avoid er-roneous treatment.
{"title":"Evaluation of myocardial infarction by a 12-lead routine electrocardiogram: a case report of an ST-segment elevation.","authors":"Huayong Jin, Lijiang Ding, Binglei Li, Jianming Zhang","doi":"10.54817/ic.v64n4a10","DOIUrl":"https://doi.org/10.54817/ic.v64n4a10","url":null,"abstract":"The spiked helmet sign (SHS) is a type of ST-segment elevation associated with critical cardiac disease and a high risk of death. We report a case of SHS caused by an ECG artifact. A 60-year-old male patient presented to the clinic after suffering an electric shock. The initial 12-lead routine elec-trocardiogram showed an SHS. The patient received appropriate intravenous fluid replacement therapy, and after 30 minutes, the ST-T changes of the 12-lead electrocardiogram were all restored to normal. The patient was discharged after a 24-hour observation period in the emergency room.Recent studies have pointed out that there may be two different types of SHS. One is the mechanical factor, and the other is the significant prolongation of the QT interval. The two types have different clinical significance. In our report, the radial artery of the patient’s right wrist pulsed strongly, and after the occurrence of SHS, the SHS disappeared after adjusting the contact position of the electrode in his right arm. This SHS caused by mechanical traction was an ECG artifact.Although the SHS may be an essential indicator of critical illness, there are mechanical factors that lead to the appearance of ECG artifacts. Therefore, in clinical work, obtaining a complete medical history and primary conditions of the patient at the time of ECG sampling is necessary to help the diagnosis and thus avoid er-roneous treatment.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"178 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139248713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SARS-CoV-2 es el agente causal de la pandemia actual de la enfermedad por coronavirus 2019 (COVID-19). Al igual que otros coronavirus respiratorios, el SARS-CoV-2 se transmite principalmente a través de gotitas respiratorias liberadas de una persona infectada. La fisiopatología de la infección por SARS-CoV-2 es semejante a la de la infección por SARS-CoV, con respuestas inflamatorias agresivas lo que genera fuertes daños a las vías respiratorias. En esta revisión abordamos la importancia de la respuesta inmunitaria innata en la fisiopatología de la COVID-19, con especial énfasis en la activación del inflamasoma y la consecuente muerte celular por piroptosis, dos elementos esenciales que podrían explicar la exacerbada respuesta inflamatoria que se observa en algunos pacientes.
{"title":"Inflamasoma, piroptosis y su posible relación con la fisiopatología de la COVID-19","authors":"Adriana Pedreáñez, J. Mosquera, Nelson Muñoz","doi":"10.22209/ic.v61n3a07","DOIUrl":"https://doi.org/10.22209/ic.v61n3a07","url":null,"abstract":"SARS-CoV-2 es el agente causal de la pandemia actual de la enfermedad por coronavirus 2019 (COVID-19). Al igual que otros coronavirus respiratorios, el SARS-CoV-2 se transmite principalmente a través de gotitas respiratorias liberadas de una persona infectada. La fisiopatología de la infección por SARS-CoV-2 es semejante a la de la infección por SARS-CoV, con respuestas inflamatorias agresivas lo que genera fuertes daños a las vías respiratorias. En esta revisión abordamos la importancia de la respuesta inmunitaria innata en la fisiopatología de la COVID-19, con especial énfasis en la activación del inflamasoma y la consecuente muerte celular por piroptosis, dos elementos esenciales que podrían explicar la exacerbada respuesta inflamatoria que se observa en algunos pacientes.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88587063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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