{"title":"Transfer agent of immunity. I. Immune ribonucleic acid which induces antibody formation to Salmonella flagella.","authors":"S Mitsuhashi, S Kurashige, M Kawakami, T Nojima","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"12 3","pages":"261-8"},"PeriodicalIF":0.0,"publicationDate":"1968-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16389829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rhapidosomes of Clostridium botulinum type E..","authors":"H Iida, K Inoue","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"12 3","pages":"353-5"},"PeriodicalIF":0.0,"publicationDate":"1968-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15978080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Drug resistance of staphylococci. IX. Inducible resistance to macrolide antibiotics in Staphylococcus aureus.","authors":"H Hashimoto, H Oshima, S Mitsuhashi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"12 3","pages":"321-7"},"PeriodicalIF":0.0,"publicationDate":"1968-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15344127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Inaba, Y Tanaka, K Sato, H Ito, Y Ito, T Omori, M Matumoto
{"title":"Bovine adenovirus. II. A serotype, Fukuroi, recovered from Japanese cattle.","authors":"Y Inaba, Y Tanaka, K Sato, H Ito, Y Ito, T Omori, M Matumoto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"12 2","pages":"219-29"},"PeriodicalIF":0.0,"publicationDate":"1968-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16389726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bovine adenovirus. I. Recovery of a serotype, Nagano, from Japanese cattle.","authors":"Y Tanaka, Y Inaba, Y Ito, T Omori, M Matsumoto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"12 1","pages":"77-95"},"PeriodicalIF":0.0,"publicationDate":"1968-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16387121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on Pathogenic leptospirae. V. Growth of pathogenic leptospirae on lipid fractions obtained from acid-fast bacilli.","authors":"Y Yanagihara, I Mifuchi, I Azuma, Y Yamamura","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"12 1","pages":"103-10"},"PeriodicalIF":0.0,"publicationDate":"1968-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16060819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1968-01-01DOI: 10.1111/J.1348-0421.1968.TB00372.X
Y. Yanagihara, I. Mifuchi, I. Azuma, Y. Yamamura
Effect of Tween 80 on the growth of Leptospira canicola strain Utrecht and L. icterohaemorrhagiae strain Mikawajima was examined. The suspension of washed leptospira was inoculated into modified Korthof's basal medium containing varied amounts of Tween 80 and cultured at 30 C. Cell numbers were counted by using Petroff-Hausser counting chamber every other day. Optimum Tween 80 concentrations for L. canicola were 0.0125 and 0.025%. Cell counts in the second sub-cultures reached 108 per ml the same as the primary cultures. Generation time of L. canicola in 0.025% Tween 80 medium was about 13 hours. Growth of L. icterohaemorrhagiae was inhibited at concentrations greater than 0.0125 per cent. Cell numbers increased about 4 times at concentration of 0.0000125% Tween 80. L. canicola utilizes Tween 80 as a nutrient while L. icterohaemorrhagiae appears sensitive to it. A difference of more than 1,000 times in maximal growth-supporting concentration between L. canicola and L. icterohaemorrhagiae exists. This difference appears to be caused by difference in surface structure and metabolic requirements.
{"title":"Studies on Pathogenic leptospirae. V. Growth of pathogenic leptospirae on lipid fractions obtained from acid-fast bacilli.","authors":"Y. Yanagihara, I. Mifuchi, I. Azuma, Y. Yamamura","doi":"10.1111/J.1348-0421.1968.TB00372.X","DOIUrl":"https://doi.org/10.1111/J.1348-0421.1968.TB00372.X","url":null,"abstract":"Effect of Tween 80 on the growth of Leptospira canicola strain Utrecht and L. icterohaemorrhagiae strain Mikawajima was examined. The suspension of washed leptospira was inoculated into modified Korthof's basal medium containing varied amounts of Tween 80 and cultured at 30 C. Cell numbers were counted by using Petroff-Hausser counting chamber every other day. Optimum Tween 80 concentrations for L. canicola were 0.0125 and 0.025%. Cell counts in the second sub-cultures reached 108 per ml the same as the primary cultures. Generation time of L. canicola in 0.025% Tween 80 medium was about 13 hours. Growth of L. icterohaemorrhagiae was inhibited at concentrations greater than 0.0125 per cent. Cell numbers increased about 4 times at concentration of 0.0000125% Tween 80. L. canicola utilizes Tween 80 as a nutrient while L. icterohaemorrhagiae appears sensitive to it. A difference of more than 1,000 times in maximal growth-supporting concentration between L. canicola and L. icterohaemorrhagiae exists. This difference appears to be caused by difference in surface structure and metabolic requirements.","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"8 1","pages":"103-10"},"PeriodicalIF":0.0,"publicationDate":"1968-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87751161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1967-03-01DOI: 10.1111/J.1348-0421.1967.TB00338.X
M. Tsukamura
A statistical method is proposed for recognition of a bacterial species or for differentiation of two groups of bacterial strains. Comparison between two groups is done by the “t”-test. When the mean S-values (similarity value) of two groups are A and B, and the mean S-value for all possible combinations between strains of both groups is S, a condition necessary for defining the two groups as different species is to demonstrate the existence of equations A > S and B > S. Unless this condition is fulfilled, the two groups should be considered unseparable. The condition necessary for recognition of two groups as one species is to demonstrate the existence of equations A:= S and B = S. A few examples of the test were shown using the mycobacteria, and it was suggested that this statistical method is useful in the recognition of a species.
{"title":"A Statistical Approach to the Definition of Bacterial Species","authors":"M. Tsukamura","doi":"10.1111/J.1348-0421.1967.TB00338.X","DOIUrl":"https://doi.org/10.1111/J.1348-0421.1967.TB00338.X","url":null,"abstract":"A statistical method is proposed for recognition of a bacterial species or for differentiation of two groups of bacterial strains. Comparison between two groups is done by the “t”-test. When the mean S-values (similarity value) of two groups are A and B, and the mean S-value for all possible combinations between strains of both groups is S, a condition necessary for defining the two groups as different species is to demonstrate the existence of equations A > S and B > S. Unless this condition is fulfilled, the two groups should be considered unseparable. The condition necessary for recognition of two groups as one species is to demonstrate the existence of equations A:= S and B = S. A few examples of the test were shown using the mycobacteria, and it was suggested that this statistical method is useful in the recognition of a species.","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"1 2","pages":"213-220"},"PeriodicalIF":0.0,"publicationDate":"1967-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91442994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1967-03-01DOI: 10.1111/J.1348-0421.1967.TB00339.X
Y. Onozawa, K. Kumagai, N. Ishida
When either colistin at 1,000 μg/ml or sulfisomezole at 125 μg/ml was used separately, growth of a strain of Proteus mirabilis was not inhibited. However, when 1 μg/ml of colistin and 25 μg/ml of sulfisomezole were used together in agar media, growth was inhibited. The synergistic action of colistin and sulfisomezole was also demonstrated in broth culture, when a smaller inoculum such as 106 cells/ml was used. The lethal and lytic effect of this synergism parallels the characteristic effect of colistin towards colistin-sensitive gram-negative organisms. When the mode of this synergistic action was analyzed by adding each compound in sequence to a growing culture of Proteus, it was found that growth of organism for about 4 generations in the presence of sulfisomezole was a prerequisite for revealing the lethal and lytic effects of colistin. In cultures where these two compounds were present at the beginning of incubation, the synergistic effect was abolished by the addition of p-aminobenzoic acid (PABA) at an early stage of incubation, but not at a late stage. Methionine, serine, and betaine, when used together, had the same effect as PABA. An insufficiency of the three compounds induced by sulfisomezole, was considered to afford the receptor site of colistin to Proteus.
{"title":"Mode of Synergism between Colistin and Sulfisomezole in Inhibiting the Growth of Proteus Organism","authors":"Y. Onozawa, K. Kumagai, N. Ishida","doi":"10.1111/J.1348-0421.1967.TB00339.X","DOIUrl":"https://doi.org/10.1111/J.1348-0421.1967.TB00339.X","url":null,"abstract":"When either colistin at 1,000 μg/ml or sulfisomezole at 125 μg/ml was used separately, growth of a strain of Proteus mirabilis was not inhibited. However, when 1 μg/ml of colistin and 25 μg/ml of sulfisomezole were used together in agar media, growth was inhibited. The synergistic action of colistin and sulfisomezole was also demonstrated in broth culture, when a smaller inoculum such as 106 cells/ml was used. The lethal and lytic effect of this synergism parallels the characteristic effect of colistin towards colistin-sensitive gram-negative organisms. When the mode of this synergistic action was analyzed by adding each compound in sequence to a growing culture of Proteus, it was found that growth of organism for about 4 generations in the presence of sulfisomezole was a prerequisite for revealing the lethal and lytic effects of colistin. In cultures where these two compounds were present at the beginning of incubation, the synergistic effect was abolished by the addition of p-aminobenzoic acid (PABA) at an early stage of incubation, but not at a late stage. Methionine, serine, and betaine, when used together, had the same effect as PABA. An insufficiency of the three compounds induced by sulfisomezole, was considered to afford the receptor site of colistin to Proteus.","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"86 1","pages":"221-227"},"PeriodicalIF":0.0,"publicationDate":"1967-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74611490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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