Pub Date : 1967-02-01DOI: 10.1111/J.1348-0421.1967.TB00325.X
Y. Nakayama
The zymogram patterns of esterases and catalases of mycobacterial strains were studied using the thin layer agar electrophoresis. Though there were some variations, Mycobacterium hominis, M. bovis, M. kansasii, M. fortuitum, M. runyonii, M. avium, M. phlei and M. smegmatis seemed to show species-specific patterns consisting of 2 to 6 esterase bands and one or more catalase bands. The patterns of scotochromogens and nonchromogens were rather variable.
{"title":"The Electrophoretical Analysis of Esterase and Catalase and Its Use in Taxonomical Studies of Mycobacteria","authors":"Y. Nakayama","doi":"10.1111/J.1348-0421.1967.TB00325.X","DOIUrl":"https://doi.org/10.1111/J.1348-0421.1967.TB00325.X","url":null,"abstract":"The zymogram patterns of esterases and catalases of mycobacterial strains were studied using the thin layer agar electrophoresis. Though there were some variations, Mycobacterium hominis, M. bovis, M. kansasii, M. fortuitum, M. runyonii, M. avium, M. phlei and M. smegmatis seemed to show species-specific patterns consisting of 2 to 6 esterase bands and one or more catalase bands. The patterns of scotochromogens and nonchromogens were rather variable.","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"9 1","pages":"95-101"},"PeriodicalIF":0.0,"publicationDate":"1967-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87638569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1967-02-01DOI: 10.1111/J.1348-0421.1967.TB00327.X
O. Sakaguchi, Sigeo Suzuki, Masuko Suzuki, H. Sunayama
Crude mannans extracted from Candida albicans and Saccharomyces cerevisiae by autoclaving yeast cells in citrate buffer (pH 7.0) according to Peat's method, were fractionated repeatedly by column chromatography on DEAE-Sephadex, acetate form, yielding neutral and acidic mannans. The former fraction showed a single peak by boundary electrophoresis and ultracentrifugal analysis, while the latter contained small amounts of phosphorus and protein. Using purified mannans as controls, various serological experiments were carried out with mannan antigens extracted from C. albicans with 45% phenol water and with 3% NaOH. No remarkable differences were observed in the antigenic activity of 4 mannan antigens from C. albicans, and the purified mannan exhibited very high antigenic activity. It was found that the mannan of S. cerevisiae was antigenically less specific than that of C. albicans mannan. The difference in serological specificity between mannans of both species may reflect not only differences in mannopyranose linkages but differences in the structure of the macromolecules.
{"title":"Biochemical and Immunochemical Studies of Fungi","authors":"O. Sakaguchi, Sigeo Suzuki, Masuko Suzuki, H. Sunayama","doi":"10.1111/J.1348-0421.1967.TB00327.X","DOIUrl":"https://doi.org/10.1111/J.1348-0421.1967.TB00327.X","url":null,"abstract":"Crude mannans extracted from Candida albicans and Saccharomyces cerevisiae by autoclaving yeast cells in citrate buffer (pH 7.0) according to Peat's method, were fractionated repeatedly by column chromatography on DEAE-Sephadex, acetate form, yielding neutral and acidic mannans. The former fraction showed a single peak by boundary electrophoresis and ultracentrifugal analysis, while the latter contained small amounts of phosphorus and protein. Using purified mannans as controls, various serological experiments were carried out with mannan antigens extracted from C. albicans with 45% phenol water and with 3% NaOH. No remarkable differences were observed in the antigenic activity of 4 mannan antigens from C. albicans, and the purified mannan exhibited very high antigenic activity. It was found that the mannan of S. cerevisiae was antigenically less specific than that of C. albicans mannan. The difference in serological specificity between mannans of both species may reflect not only differences in mannopyranose linkages but differences in the structure of the macromolecules.","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"7 1","pages":"119-128"},"PeriodicalIF":0.0,"publicationDate":"1967-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74269259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Colicine typing of Shigella sonnei. I. Principle, technique, selection of indicator strains, and foundations for a typing scheme.","authors":"T Naito, M Kono, N Fujise, Y Yakushiji, Y Aoki","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"10 1","pages":"13-22"},"PeriodicalIF":0.0,"publicationDate":"1966-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15512230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Drug resistance to staphylococci. 3. Resistance to some macrolide antibiotics and inducible system.","authors":"M Kono, H Hashimoto, S Mitsuhashi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"10 1","pages":"59-66"},"PeriodicalIF":0.0,"publicationDate":"1966-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16382169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1966-01-01DOI: 10.1111/J.1348-0421.1966.TB00287.X
T. Naito, M. Kono, N. Fujise, Y. Yakushiji, Y. Aoki
Colicine typing of Shigella sonnei using the methods of Naito et al. and Abbott and Shannon were performed in parallel on a large number of epidemic strains, the indicator strains used in both methods, and on stock strains used to test the accuracy of the indicators. The following results were obtained after typing 462 epidemic strains: by Naito's method, 18 strains were in A1, 87 in A2, 33 in B1, 253 in B2, 38 in C1, 3 in C2, 2 in D, and 3 in E, and 25 strains were unclassifiable; while by Abbott and Shannon's method, using the present authors' simplified designation, 189 strains were in type 6/11, 124 in type 4/14, 85 in type O, and 41 in type 8, and 23 strains were either in other types or found unclassifiable. Naito's type A was in large part, equivalent to Abbott and Shannon's type O, but included some type 6/11 and type 4/14 strains. Naito's type B consisted of types 6/11, 4/14, 3A and 13, and type E consisted of 1A, IB, 3, 5, 9, and 10. Type C coincided with type 8 and type D with type 12. In addition, there were strains determined to be Abbott and Shannon's types 6/11, 4/14, 2, 7 and 9. The Abbott and Shannon's method revealed the possibilities of these strains to be classified in further detail. This is considered to be attributable not merely to the lack of indicators in the Naito's method corresponding to those of Abbott and Shannon's, but also to insufficient production of certain colicines in the Naito's method. Because of the type distribution found in Japan at present, further investigation should be done so that types 6 and 11, and types 4 and 14 can be separated.
{"title":"Colicine typing of Shigella sonnei. I. Principle, technique, selection of indicator strains, and foundations for a typing scheme.","authors":"T. Naito, M. Kono, N. Fujise, Y. Yakushiji, Y. Aoki","doi":"10.1111/J.1348-0421.1966.TB00287.X","DOIUrl":"https://doi.org/10.1111/J.1348-0421.1966.TB00287.X","url":null,"abstract":"Colicine typing of Shigella sonnei using the methods of Naito et al. and Abbott and Shannon were performed in parallel on a large number of epidemic strains, the indicator strains used in both methods, and on stock strains used to test the accuracy of the indicators. The following results were obtained after typing 462 epidemic strains: by Naito's method, 18 strains were in A1, 87 in A2, 33 in B1, 253 in B2, 38 in C1, 3 in C2, 2 in D, and 3 in E, and 25 strains were unclassifiable; while by Abbott and Shannon's method, using the present authors' simplified designation, 189 strains were in type 6/11, 124 in type 4/14, 85 in type O, and 41 in type 8, and 23 strains were either in other types or found unclassifiable. Naito's type A was in large part, equivalent to Abbott and Shannon's type O, but included some type 6/11 and type 4/14 strains. Naito's type B consisted of types 6/11, 4/14, 3A and 13, and type E consisted of 1A, IB, 3, 5, 9, and 10. Type C coincided with type 8 and type D with type 12. In addition, there were strains determined to be Abbott and Shannon's types 6/11, 4/14, 2, 7 and 9. The Abbott and Shannon's method revealed the possibilities of these strains to be classified in further detail. This is considered to be attributable not merely to the lack of indicators in the Naito's method corresponding to those of Abbott and Shannon's, but also to insufficient production of certain colicines in the Naito's method. Because of the type distribution found in Japan at present, further investigation should be done so that types 6 and 11, and types 4 and 14 can be separated.","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"72 1","pages":"13-22"},"PeriodicalIF":0.0,"publicationDate":"1966-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86274013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antibiotic resistant group A streptococci. I. Acquired in vitro resistance to penicillin, mitomycin C. tetracycline and streptomycin.","authors":"M Yoshioka, T Kunii","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"9 2","pages":"87-99"},"PeriodicalIF":0.0,"publicationDate":"1965-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16404396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transduction of drug-resistances in Staphylococcus. II. Transduction of chloramphenicol-resistance in both Staphylococcus aureus and Staphylococcus epidermidis by typing phage 80.","authors":"S Goto, C Niwa, S Kuwahara","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"9 1","pages":"15-9"},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15326695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ELECTRON MICROSCOPIC STUDIES ON THE CYTOLOGICAL STRUCTURE OF TRICHOPHYTON MENTAGROPHYTES.","authors":"T TSUKAHARA, A SATO, R OKADA","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":"8 ","pages":"85-96"},"PeriodicalIF":0.0,"publicationDate":"1964-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23783422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IMMUNOLOGICAL STUDIES ON \"SURFACE ANTIGEN\" EXTRACTED FROM STAPHYLOCOCCUS AUREUS. II. ANTISURFACE SUBSTANCE ANTIBODIES IN THE SERUM OF NORMAL PERSONS AND PATIENTS.","authors":"K YOSHIDA, S I MIZUNARI, H HATTORI","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":" ","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"1964-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40755662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IMMUNOLOGICAL STUDIES ON \"SURFACE ANTIGEN\" EXTRACTED FROM STAPHYLOCOCCUS AUREUS. I. ISOLATION OF THE ANTIGEN AND PROPERTIES OF THE IMMUNE RABBIT SERUM.","authors":"K YOSHIDA, S I MIZUNARI, H HATTORI","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":14559,"journal":{"name":"Japanese journal of microbiology","volume":" ","pages":"67-74"},"PeriodicalIF":0.0,"publicationDate":"1964-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40755661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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