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Kidney regeneration in Aphanius furcatus (Teleostei: Aphaniidae) after damage induced by gentamicin and evaluation of vitamin C in toxicity reduction 庆大霉素致肾损伤后的肾再生及维生素C的降毒作用
Pub Date : 2019-03-25 DOI: 10.22034/IJI.V6I1.351
M. Motamedi, A. Iranmanesh, A. Teimori
Gentamicin is known to stimulate the production of reactive oxygen species and lead to renal damage in some groups of vertebrates through oxidative stress. The kidney regeneration of the killifish Aphanius furcatus was studied after gentamicin-induced oxidative stress. In addition, we evaluated the possible role of vitamin C as an antioxidant in the reduction of renal toxicity. A total of 24 specimens (3.5-4cm in total length) were divided into four groups: In group-I two fish specimens treated with 7.5µl of normal saline injection; in group-II, 10 specimens received 10µg/g body weight (b.w) gentamicin; in group-III, two fish were treated with totally 60µg/g b.w vitamin C; and in group-IV, 10 specimens received both 10µg/g gentamicin and 60µg/g b.w vitamin C. The kidney tissues of group-I, III and IV were sampled during the nephrotoxicity phase (i.e., 10 hours post injection (hpi) to 5 days post injection (dpi)) and kidney tissues of group-II sampled daily for 10 dpi until regeneration completed. The kidney regeneration of A. furcatus through nephron neogenesis was detected by newly formed nephron on 8 dpi, and it is completed on 10 dpi. Our histological results in gentamicin-induced oxidative stress (group-II) clearly showed that the most severe damage is occurred in the kidney tissue within 1-4 dpi. However, in the specimens that antioxidant therapy supplied with vitamin C (group-IV), damage effect of gentamicin has remarkably repressed within these days. The outcome of this study revealed that administration of vitamin C is effective in reduction of gentamicin-induced nephrotoxicity in A. furcatus . Vitamin C reduces the toxicity effect of gentamicin by reduction of free radicals and reactive oxygen species (ROS) through its antioxidant activity. Therefore, using vitamin C together with gentamicin enhances the body resistance to the stress condition and reduces the toxicity effect of gentamicin.
已知庆大霉素可以刺激活性氧的产生,并通过氧化应激导致某些脊椎动物的肾脏损伤。研究了庆大霉素诱导的氧化应激对大鲵肾再生的影响。此外,我们评估了维生素C作为抗氧化剂在减少肾毒性中的可能作用。24个标本(总长度3.5 ~ 4cm)分为4组:1组2个鱼标本,注射生理盐水7.5µl;ii组10只,给予10µg/g体重(b.w)庆大霉素;在第三组,2只鱼被给予60µg/g b.w的维生素C;IV组,10个标本同时接受10µg/g庆大霉素和60µg/g b.w维生素c。在肾毒性阶段(即注射后10小时(hpi)至注射后5天(dpi)), i、III和IV组的肾脏组织每天采样10 dpi,直到再生完成。在第8 dpi时,新形成的肾元检测到树蛙通过肾元新生进行肾脏再生,在第10 dpi时完成肾元再生。庆大霉素诱导的氧化应激(ii组)的组织学结果清楚地表明,肾脏组织在1-4 dpi内发生最严重的损伤。然而,在提供维生素C (iv族)抗氧化治疗的标本中,庆大霉素的损伤作用在这些天内明显受到抑制。本研究的结果显示,维生素C的管理是有效的减少庆大霉素引起的肾毒性在A. furcatus。维生素C通过其抗氧化活性减少自由基和活性氧(ROS),从而降低庆大霉素的毒性作用。因此,维生素C与庆大霉素联合使用可以增强机体对应激条件的抵抗力,降低庆大霉素的毒性作用。
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引用次数: 3
Evaluation of health status of Indian major and minor carps cultured in West Bengal, India 在印度西孟加拉邦养殖的印度大、小鲤鱼的健康状况评价
Pub Date : 2019-03-25 DOI: 10.22034/IJI.V6I1.313
T. Abraham, K. Ramudu, S. Rajesh, Sayani Banerjee, Harresh Adikesavalu, G. Dash, T. S. Nagesh
In Indian freshwater aquaculture, which largely depends on carps, diseases have become a major problem. This study evaluated the health status of Catla catla, Labeo rohita, Cirrhinus mrigala and Labeo bata cultured in West Bengal, India by determining the Health Assessment Index (HAI) and coefficient of variation (CV) of HAI values of each species as well as the superoxide anion production. The annual mean HAI value was the highest for L. rohita (77.51) and least for L. bata (55.30). The highest and lowest CV of HAI values were recorded for L. bata (95.80) and C. mrigala (52.55), respectively . The HAI values correlated negatively ( P <0.05) with temperature. The HAI distinguished successfully among the species, seasons and sampling areas on the grounds of temperature and water quality, with higher index values recorded in carps grown in poorly managed ponds. Labeo bata exhibited higher immune responsiveness compared to other carps. Labeo rohita was the most susceptible to diseases. The results on the high production of oxygen radicals by phagocytic cells of L. bata together with low HAI and high CV values suggested that this species may resist biotic and abiotic stressors well under culture conditions. The results suggested that HAI and superoxide anion production can be used to assess the health status of carps under the culture conditions.
在很大程度上依赖鲤鱼的印度淡水水产养殖中,疾病已成为一个主要问题。本研究通过测定印度西孟加拉邦养殖的鲇鱼(Catla Catla)、罗希塔鱼(Labeo rohita)、马柳鱼(Cirrhinus mrigala)和巴塔鱼(Labeo bata)的健康评价指数(HAI)和HAI值变异系数(CV),以及超氧阴离子(superoxide阴离子)的产生量,对其健康状况进行了评价。年平均HAI值以罗氏L.最高(77.51),bata最低(55.30)。其中,巴塔草(95.80)和金缕草(52.55)的HAI值CV值最高、最低。HAI值与温度呈负相关(P <0.05)。该指数成功地根据温度和水质区分了不同的种类、季节和采样地区,在管理不善的池塘中生长的鲤鱼的指数值较高。与其他鲤鱼相比,青鱼表现出更高的免疫反应性。Labeo rohita是最容易患病的。L. bata吞噬细胞产生大量的氧自由基,同时具有较低的HAI和较高的CV值,表明该物种在培养条件下可以很好地抵抗生物和非生物胁迫。结果表明,在不同的培养条件下,HAI和超氧阴离子的产生可以用来评价鲤鱼的健康状况。
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引用次数: 0
Morphological characteristics of otolith for four fish species in the Edremit Gulf, Aegean Sea, Turkey 土耳其爱琴海埃德雷姆湾四种鱼类耳石的形态特征
Pub Date : 2019-01-27 DOI: 10.22034/IJI.V5I4.312
H. Bal, D. Türker, K. Zengin
Otolith morphology is important for species identification, fishery management and stock assessment. In this study, otolith (Sagitta) morphology of four fish species viz. Pomatomus saltatrix , Sarpa salpa , Trachurus trachurus , and Belone belone was investigated. The length (mm), width (mm), area (mm 2 ) and perimeter (mm) measurements of the otoliths were made and four different shape indices, including form factor (F F ), aspect ratio (A R ), roundness (R D ) and circularity (C I ) were calculated. In addition, relationships between total fish length and otolith morphology were investigated. Measurements of sagittal otoliths of four species were obtained by image analysis using tri-ocular microscope. Based on the results, the morphometric measurements and four examined shape factors of otolith varied among species. Coefficients of correlations (r) between total fish length and otolith morphology and otolith length-otolith morphology were generally highly significant ( P <0.05) for studied fishes. The results show that the shape indices were significantly different in analyzed species even they indicated a similar pattern with maximal otolith length. These data provide information for species identification using sagittal otoliths in the fossils and diets of fish predators and also will contribute to the region's sustainable fisheries management.
耳石形态对物种识别、渔业管理和种群评估非常重要。在本研究中,对四种鱼类的耳石(矢状体)形态进行了研究,这些鱼类分别是:盐斑鱼、萨尔帕鱼、气管龙和贝隆贝隆鱼。对耳石的长度(mm)、宽度(mm),面积(mm2)和周长(mm)进行了测量,并计算了四种不同的形状指数,包括形状因子(F F)、纵横比(A R)、圆度(R D)和圆度(C I)。此外,还研究了鱼类总长度和耳石形态之间的关系。用三眼显微镜对四种动物的矢状耳石进行了图像分析。根据这些结果,耳石的形态测量和四个被检查的形状因子在物种之间存在差异。鱼类总长度与耳石形态和耳石长度耳石形态之间的相关系数(r)对于所研究的鱼类来说通常非常显著(P<0.05)。结果表明,被分析物种的形状指数存在显著差异,即使它们在最大耳石长度上表现出相似的模式。这些数据为利用鱼类捕食者化石和饮食中的矢状耳石进行物种识别提供了信息,也将有助于该地区的可持续渔业管理。
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引用次数: 3
Otolith shape analyses of Squalius cephalus (Linnaeus, 1758) (Actinopterygii: Cyprinidae) inhabiting four inland water bodies of the middle Black Sea region, Turkey 栖息在土耳其黑海中部四个内陆水体的头角鲨(Linnaeus,1758)(Actinopterygii:Cyprinidae)的耳石形状分析
Pub Date : 2019-01-26 DOI: 10.22034/IJI.V5I4.311
M. Özpiçak, S. Saygın, Aykut Aydın, Enes Hançer, S. Yılmaz, N. Polat
This study was carried out to determine the intra- and inter- population variations of chub, Squalius cephalus , utricular and lagenar otolith shapes sampled from four localities in the Black Sea region. Otolith shape indices, including Form Factor (FF), Circularity (C), Roundness (RO), Rectangularity (RE), Aspect Ratio (AR) and Ellipticity (E) were used for otolith shape analyses and multivariate analyzes (Canonical Discriminant Analysis) were used to assess interspecies variations. The FF and C were found statistically significant for lapillus, but not for asteriscus otoliths in the four habitats. Furthermore, RO was similar for all localities for both asteriscus and lapillus. CDA results showed that 66.5% of the chub individuals were correctly classified. The results indicated otolith shape can be used as a suitable tool to discriminate chub populations.
本研究旨在确定从黑海地区四个地点采集的圆周鱼、头角鲨、椭圆囊和拉格纳尔耳石形状的种群内和种群间变化。耳石形状指数,包括形状因子(FF)、圆形度(C)、圆度(RO)、矩形度(RE)、长宽比(AR)和椭圆度(E)用于耳石形状分析,多元分析(典型判别分析)用于评估种间变异。FF和C在四个栖息地中被发现对珊瑚有统计学意义,但对紫苏耳石没有统计学意义。此外,所有地区的紫苏和拉普卢的RO相似。CDA结果显示,66.5%的胖乎乎的个体被正确分类。结果表明,耳石形状可以作为一种合适的工具来鉴别胖乎乎的种群。
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引用次数: 12
Age, growth, sex ratio, spawning season and fecundity of Capoeta razii from Sefidroud River in the southern Caspian Sea basin 里海盆地南部Sefidroud河斑螯蟹的年龄、生长、性别比、产卵季节和繁殖力
Pub Date : 2019-01-25 DOI: 10.22034/IJI.V5I4.287
Kobra Ghasemzadeh-Sarcheshmeh, H. Mousavi-Sabet, M. Sattari, S. Vatandoust, M. Ahmadnezhad
Some aspects of reproductive biology of Capoeta razii , an endemic cyprinid species from the Sefid River in the southern Caspian Sea basin, were studied by monthly sampling throughout a year. Age, growth, sex ratio, spawning season, fecundity, gonado-somatic, modified gonado-somatic and Dobriyal indices were estimated. The sex ratio (F: M) was obtained as 1.00:2.63, and the percentage of males was greater than females. The mature males and females were longer than 88 and 148 mm in total length, and 1 + and 2 + in age, respectively. The spawning of C. razii took place from late May to late August when the water temperature was between 19.7 and 22.8 ◦ C. At the beginning of the reproduction period, the average gonado-somatic index values were 10.0% ranging from 4.03% to 15.90% in ripe females. The estimated gonadal indices showed that the ripe males were ready to spawn earlier than females. The average egg diameter was 0.99±0.65, ranging from 0.2 to 2.3 mm. The averages absolute and relative fecundity were 4386±2174 and 66.3±37.8, respectively. The absolute fecundity was significantly related to body weight and gonad weight.
通过一年中的每月采样,研究了来自里海南部流域Sefid河的特有塞浦路斯物种Capoeta razii的生殖生物学的某些方面。估计了年龄、生长、性别比、产卵季节、繁殖力、性腺体细胞、改良性腺体细胞和多鱼指数。性别比(F:M)为1.00:2.63,男性的比例大于女性。成熟雄性和雌性的总长度分别超过88和148mm,年龄分别为1+和2+。拉齐C.razii的产卵发生在5月下旬至8月下旬,当时水温在19.7至22.8之间◦ C.在生殖期开始时,成熟雌性的性腺-体细胞指数平均值为10.0%,范围为4.03%至15.90%。估计的性腺指数表明,成熟的雄性比雌性更早做好产卵的准备。平均卵径为0.99±0.65,范围为0.2至2.3mm。平均绝对繁殖力和相对繁殖力分别为4386±2174和66.3±37.8。绝对繁殖力与体重和性腺重量显著相关。
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引用次数: 1
Review and re-description of Sabanejewia species in Iran (Teleostei: Cobitidae) 伊朗Sabanejewia物种的回顾与重新描述(遥尾目:眼镜蛇科)
Pub Date : 2019-01-25 DOI: 10.22034/IJI.V5I4.322
G. Sayyadzadeh, K. Abbasi, H. Esmaeili
The Iranian species of the genus Sabanejewia are reviewed based on morphological, osteological and molecular characters of the mtDNA COI barcode region. Two species, Sabanejewia aurata and Sabanejewia caspia are recognized and diagnoses are presented for both species. They are distinguishable through body pattern, length of caudal peduncle, snout and post orbital distance, shape of scale and suborbital spine, caudal complex skeleton and also the number of fixed, diagnostic nucleotide substitutions. Here we re-describe both Iranian spined loaches.
本文从形态学、骨学和分子生物学的角度对伊朗种Sabanejewia的mtDNA COI条形码区进行了综述。对aurata Sabanejewia和caspia Sabanejewia这两个物种进行了识别和诊断。它们可以通过体型、尾柄长度、鼻和眶后距离、鳞片和眶下棘的形状、尾复合骨架以及固定的、诊断性的核苷酸取代的数量来区分。这里我们重新描述两种伊朗棘泥鳅。
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引用次数: 3
First report of a rare snake eel, Allips concolor McCosker, 1972 (Anguilliformes: Ophichthidae) from Indian waters 印度水域罕见蛇鳗首次报告,Allips concolor McCosker, 1972(鳗形目:蛇鳗科)
Pub Date : 2019-01-08 DOI: 10.22034/IJI.V5I4.286
A. Mohapatra, S. R. Mohanty, S. S. Mishra, D. Ray
The monospecific genus Allips is reported here for the first time from Indian waters, from a specimen collected from the sea grass bed outside the Nalban bird Sanctuary, Chilika lagoon, India, with its morphometric characters, vertebrae and teeth pattern.  The rare small eye snake eel A. concolor was earlier described by McCosker, 1972 on the basis of a single specimen. After that, there are very rare reports on this particular species especially on its morphometry after McCosker (1972). This paper extends the range of A. concolor McCosker, 1972 from eastern Andaman Sea and Northern Territory, Australia to Indian waters. A detailed discussion on the distribution and habitat is also provided in this paper.
本文首次在印度水域报道了单种Allips属,该属标本采集于印度Chilika泻湖Nalban鸟类保护区外的海草床,具有形态特征、椎骨和牙齿图案。McCosker早在1972年就在单个标本的基础上描述了罕见的小眼蛇鳗a . concolor。在那之后,关于这一特殊物种的报道非常少,特别是在McCosker(1972)之后。本文将A. concolor McCosker, 1972的分布范围从东安达曼海和澳大利亚北部地区扩展到印度水域。本文还对其分布和生境作了详细的讨论。
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引用次数: 1
Review of the bitterlings of Iran (Family Acheilognathidae) 伊朗卤虫科(Acheilognathidae)研究进展
Pub Date : 2019-01-08 DOI: 10.22034/IJI.V5I4.321
B. Coad
The systematics, morphology, distribution, biology and economic importance of the bitterlings of Iran are described, the species is illustrated, and a bibliography on this fish in Iran is provided. There is a single species, Rhodeus amarus , found in the Caspian Sea basin and introduced to the Lake Urmia and Tigris River basins.
介绍了伊朗卤鱼的系统学、形态、分布、生物学和经济意义,并对其种类进行了说明,并提供了伊朗卤虫的参考文献。在里海盆地发现了一个单一的物种,即阿玛红,并将其引入了乌尔米亚湖和底格里斯河流域。
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引用次数: 2
First record of non-native Bluering angelfish Pomacanthus annularis (Perciformes: Pomacanthidae) from the marine water of Odisha coast, Bay of Bengal, India 印度孟加拉湾奥迪沙海岸海水中的非本地蓝环天使鱼环斑棘鲷(鲈形目:棘鲷科)的首次记录
Pub Date : 2019-01-08 DOI: 10.22034/IJI.V5I4.289
T. K. Barik, S. N. Swain, B. Sahu, B. Tripathy, Usha R. Acharya
The bluering angelfish Pomacanthus annularis (Bloch, 1787) is a well-known pomacanthid distributed in Gulf of Mannar, Indian Ocean, IndoWest Pacific: east coast of Africa, throughout Indonesia and New Guinea to New Caledonia, and in Andaman Sea. For the first time one specimen belonging to this species was caught in the Odisha coast, Bay of Bengal, India. We reviewed its distribution after compilation with available literature.
蓝天使鱼环斑棘皮鱼(Bloch,1787)是一种著名的棘皮鱼,分布在马纳尔湾、印度洋、印度西太平洋:非洲东海岸、整个印度尼西亚、新几内亚到新喀里多尼亚,以及安达曼海。在印度孟加拉湾的奥迪沙海岸首次发现了一个属于该物种的标本。我们在汇编现有文献后对其分布情况进行了审查。
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引用次数: 2
Oxynoemacheilus veyseli, a new nemacheilid species from the upper Aras River drainage of Turkey (Teleostei: Nemacheilidae) 土耳其阿拉斯河上游一新种(网须鱼目:网须鱼科)
Pub Date : 2018-10-24 DOI: 10.22034/IJI.V5I3.302
E. Çiçek, S. Eagderi, S. Sungur
A new species of nemacheilid fish, Oxynoemacheilus veyseli sp. n., is described from the upper Aras River drainage, Kars Province, Turkey. The species differs from its congeners in the Caspian Sea basin in the combination of the following characters: flank with a set of mid-lateral elongated, irregular-shaped dark brown blotches, sometimes fused, interrupted by lateral line; dorsum with three or four separated dark-brown roundish to elongated blotches predorsally and postdorsally; a deep and marked suborbital groove and many small unculi on rays of pectoral and dorsal-fin in male; small eye diameter (9.6-13.7 %HL); a short dorsal-fin base (10.1-13.3 %SL); 3-4 lateral and 3 central pores in supratemporal canal; deep body and caudal peduncle (17.3-19.4 and 10.2-11.4 %SL, respectively) and slightly emarginated caudal fin.
报道了土耳其卡尔斯省阿拉斯河上游流域的一新种拟网纲鱼类Oxynoemacheilus veyseli sp. n.。本种与里海盆地的同类不同之处在于以下特征的组合:侧面有一组中外侧细长,不规则形状的深棕色斑点,有时融合,被侧线打断;背具三个或四个分开的深棕色圆形到拉长的斑点,前背和后背;在雄性的胸鳍和背鳍上有深的和显著的亚眶沟和许多小钩;眼径小(9.6- 13.7% HL);短背鳍基部(10.1- 13.3% SL);颞上管3 ~ 4个外侧孔和3个中央孔;体深和尾端花序梗(分别为17.3- 19.4%和10.2- 11.4%)和尾鳍微缺。
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引用次数: 6
期刊
Iranian Journal of Ichthyology
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