Pub Date : 2018-05-24DOI: 10.25081/RIPB.2018.V8.3567
S. Gautam, Sanjay Kumar Navneet
M. parviflora fruit extracts was subjected for extraction with different solvents and tested for antibacterial property by agar well diffusion method against five bacterial pathogens responsible for respiratory tract infections. From this plant, all the studied extracts revealed inhibitory action towards tested microorganisms. Highest inhibition was in methanol and ranged between 15.3±0.28 to 20.6±0.57 mm with uppermost activity against S. pneumoniae and lowest against P. aeruginosa respectively. The MIC were fixed by two-fold serial dilution method and recorded for methanol extracts at 3.1212.5 mg/ml. Phytochemical study revealed the variable presence of secondary metabolites including alkaloids, flavonoids, glycosides, steroids, terpenes, saponins and tannins. From the results, it can be concluded that M. parviflora has significant bioactivity against tested microorganisms and can be utilized in drug development against respiratory ailments.
{"title":"SCREENING OF ANTIBACTERIAL AND PHYTOCHEMICAL CONSTITUENTS OF MALVA PARVIFLORA LINN. FRUIT EXTRACTS AGAINST RESPIRATORY TRACT PATHOGENS","authors":"S. Gautam, Sanjay Kumar Navneet","doi":"10.25081/RIPB.2018.V8.3567","DOIUrl":"https://doi.org/10.25081/RIPB.2018.V8.3567","url":null,"abstract":"M. parviflora fruit extracts was subjected for extraction with different solvents and tested for antibacterial property by agar well diffusion method against five bacterial pathogens responsible for respiratory tract infections. From this plant, all the studied extracts revealed inhibitory action towards tested microorganisms. Highest inhibition was in methanol and ranged between 15.3±0.28 to 20.6±0.57 mm with uppermost activity against S. pneumoniae and lowest against P. aeruginosa respectively. The MIC were fixed by two-fold serial dilution method and recorded for methanol extracts at 3.1212.5 mg/ml. Phytochemical study revealed the variable presence of secondary metabolites including alkaloids, flavonoids, glycosides, steroids, terpenes, saponins and tannins. From the results, it can be concluded that M. parviflora has significant bioactivity against tested microorganisms and can be utilized in drug development against respiratory ailments.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115041419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-01DOI: 10.25081/RIPB.2018.V8.3600
J. Jasmine, V. Balakrishnan
Diversity within and among the population of Gloriosa superba collected from five different location of Tamil Nadu State, India, were explored by using Inter Simple Sequence Repeat (ISSR) DNA sequencing method. A total of 86.27% of polymorphic bands were seen in 94 reproducible bands generated from 12 number of ISSR primer ranging between 200bp to1000bp of band width. ISSR primer produces the bands with an average polymorphism of more than ninety nine percent among all the ecotypes. The dendrograms drawn for the analysis of genetic similarity in all the ecotypes. Our studies reveals that, genetic variations among different accessions of Gloriosa superba(L) was very well identified with the help of ISSR finger printings technic.
{"title":"INTRA SPECIFIC ANALYSIS OF GLORIOSA SUPERBA (L) THROUGH ISSR FINGER PRINTING AND DNA SEQUENCING OF ECOTYPES COLLECTED FROM DIFFERENT ACCESSIONS OF TAMIL NADU STATE, INDIA","authors":"J. Jasmine, V. Balakrishnan","doi":"10.25081/RIPB.2018.V8.3600","DOIUrl":"https://doi.org/10.25081/RIPB.2018.V8.3600","url":null,"abstract":"Diversity within and among the population of Gloriosa superba collected from five different location of Tamil Nadu State, India, were explored by using Inter Simple Sequence Repeat (ISSR) DNA sequencing method. A total of 86.27% of polymorphic bands were seen in 94 reproducible bands generated from 12 number of ISSR primer ranging between 200bp to1000bp of band width. ISSR primer produces the bands with an average polymorphism of more than ninety nine percent among all the ecotypes. The dendrograms drawn for the analysis of genetic similarity in all the ecotypes. Our studies reveals that, genetic variations among different accessions of Gloriosa superba(L) was very well identified with the help of ISSR finger printings technic.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134514743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-27DOI: 10.25081/RIPB.2018.V8.3366
Samrin Shaikh, V. Shriram, T. Khare, Vinay Kumar
Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora . To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.
鸢尾草(malvacae)是一种在传统治疗中被广泛使用的药用植物。具有抗疟原虫、降血糖、降血脂、保肝、抗感染、抗氧化、抗hiv等广谱治疗作用。本研究的目的是建立该植物的细胞悬浮培养，该细胞悬浮培养可进一步用于体外生产所需的次生代谢物并进一步扩大其规模。用硫酸打破种子休眠，离体培养幼苗。在添加2,4- d (0.5 mg/L)的MS培养基中，结状外植体愈伤组织最多。所产生的愈伤组织被用作建立悬浮培养的外植体。结果表明，不添加任何培养基的MS培养基最适合建立异色菌的细胞悬浮培养。据我们所知，这是第一篇关于异色菌细胞悬浮培养建立的报道。
{"title":"Establishment of Callus and Cell Suspension Cultures of Helicteres isora L.","authors":"Samrin Shaikh, V. Shriram, T. Khare, Vinay Kumar","doi":"10.25081/RIPB.2018.V8.3366","DOIUrl":"https://doi.org/10.25081/RIPB.2018.V8.3366","url":null,"abstract":"Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora . To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126048829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-23DOI: 10.25081/RIPB.2018.V8.3750
E. Aqlan, Z. Turki, F. Shehata
The morphological and anatomical characteristics of different parts were utilized to reassess the taxonomic status in Scorpiurus L. species naturally growing in Egypt. There were significant differences among the studied species of plants and hence the results clearly showed two distinct species. Based on the studied morphological and anatomical differences S. muricatus L. is differentiated into three different varieties. These are muricatus L., laevigatus (Sibth. & Sm.) Boiss. and subvillosus (L.) Fiori. An artificial key to both species and varieties are provided.
{"title":"Taxonomic revision of the genus Scorpiurus L. (Fabaceae)","authors":"E. Aqlan, Z. Turki, F. Shehata","doi":"10.25081/RIPB.2018.V8.3750","DOIUrl":"https://doi.org/10.25081/RIPB.2018.V8.3750","url":null,"abstract":"The morphological and anatomical characteristics of different parts were utilized to reassess the taxonomic status in Scorpiurus L. species naturally growing in Egypt. There were significant differences among the studied species of plants and hence the results clearly showed two distinct species. Based on the studied morphological and anatomical differences S. muricatus L. is differentiated into three different varieties. These are muricatus L., laevigatus (Sibth. & Sm.) Boiss. and subvillosus (L.) Fiori. An artificial key to both species and varieties are provided.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117209731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-01DOI: 10.25081/ripb.2017.v7.3602
Imdad Ali Mahmood Arshad Ali Muhammad Arshad Ullah, Qaiser Nawaz, Badar-Uz-Zaman Tariq Sultan
A field experiment was carried out at the experimental farm of Soil Salinity Research Institute, Pindi Bhattian during 2016-17 to assess evaluate different Rhizobial inoculation methods on growth and yield of rice (Oryza sativa) cv. Basmati-385 under naturally salt-affected soil (pH= 8.12, ECe= 7.88 dS m-1and SAR=24.96). Seeds of rice were inoculated with Biozote–Max (Plant Growth Promoting Rhizobacteria-PGPR) produced in Soil Biology and Biotechnology Research Programme of Land Resources Research Institute, National Agricultural research Centre (NARC), Islamabad under PARC-Agrotech. Company (Pvt) Ltd. NARC, Islamabad in three ways i.e. rice seeds inoculated for direct seeding and nursery and dipping seedling roots in the solutions of these rhizobial strains comparable of control un-inoculated (control) by each inoculation method. The performances of Rhizobial strain Biozote–Max used as seed and seedling root dipping inoculation were superior to uninoculated control in all the parameters of the rice crop. Among the treatments, seed and seedling root dipping inoculation with Rhizobial strain Biozote–Max performed best in recording plant height, panicle length, number of tillers, 1000-grain weight and grain yield of rice crop. Maximum tillering was observed with all strains under different inoculation methods. Although, the strains performed better as compared to control, however, dipping of nursery roots produced significantly higher yield followed by seed inoculation for direct seeding. Overall, among all the inoculation methods seedling root dipping produced comparable paddy yield. The highest paddy yield (367 g m-2) was harvested with SBCC (M8) seed inoculation which was 13% more than that of un-inoculated (control).
{"title":"EFFECT OF INOCULATION METHODS OF BIOZOTE-MAX (PLANT GROWTH PROMOTING RHIZOBACTERIA-PGPR) ON GROWTH AND YIELD OF RICE UNDER NATURALLY SALT-AFFECTED SOIL","authors":"Imdad Ali Mahmood Arshad Ali Muhammad Arshad Ullah, Qaiser Nawaz, Badar-Uz-Zaman Tariq Sultan","doi":"10.25081/ripb.2017.v7.3602","DOIUrl":"https://doi.org/10.25081/ripb.2017.v7.3602","url":null,"abstract":"A field experiment was carried out at the experimental farm of Soil Salinity Research Institute, Pindi Bhattian during 2016-17 to assess evaluate different Rhizobial inoculation methods on growth and yield of rice (Oryza sativa) cv. Basmati-385 under naturally salt-affected soil (pH= 8.12, ECe= 7.88 dS m-1and SAR=24.96). Seeds of rice were inoculated with Biozote–Max (Plant Growth Promoting Rhizobacteria-PGPR) produced in Soil Biology and Biotechnology Research Programme of Land Resources Research Institute, National Agricultural research Centre (NARC), Islamabad under PARC-Agrotech. Company (Pvt) Ltd. NARC, Islamabad in three ways i.e. rice seeds inoculated for direct seeding and nursery and dipping seedling roots in the solutions of these rhizobial strains comparable of control un-inoculated (control) by each inoculation method. The performances of Rhizobial strain Biozote–Max used as seed and seedling root dipping inoculation were superior to uninoculated control in all the parameters of the rice crop. Among the treatments, seed and seedling root dipping inoculation with Rhizobial strain Biozote–Max performed best in recording plant height, panicle length, number of tillers, 1000-grain weight and grain yield of rice crop. Maximum tillering was observed with all strains under different inoculation methods. Although, the strains performed better as compared to control, however, dipping of nursery roots produced significantly higher yield followed by seed inoculation for direct seeding. Overall, among all the inoculation methods seedling root dipping produced comparable paddy yield. The highest paddy yield (367 g m-2) was harvested with SBCC (M8) seed inoculation which was 13% more than that of un-inoculated (control).","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129495984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-01DOI: 10.25081/RIPB.2017.V7.3604
G. Akhter, T. A. Khan
Among the major weed groups that cause huge economic losses to important cropping systems, Orobanche species are greatly devastating. Egyptian broomrape (Orobanche aegyptiaca) is a parasitic weed causing major yield loss in many field and vegetable crops and is a serious threat to Indian mustard. In this study, severalgenotypes of Indian mustard were screened in order to identify resistant genotypes against O. aegyptiaca. In the greenhouse conditions, genotypes were different in the degree of susceptibility to Broomrape. Attachment number, emergence number, and dry matter of parasitic broomrape were affected by biomass of genotype. A significant impact of the parasitism onto the dry weight of all infected mustard genotype with variable degree was observed. Broomrape attachment was observed in all the cultivated genotypes with Pusa mustard 24 being the most susceptible with the greatest number of emerged Orobanche shoots. In contrast, no emergence shoots were observed in four out of the fifteen genotype viz., Pusa Jaikisan, Pusa bold, Pusa Vijay and Pusa mustard 26 which have less attachment number and emergence number. These genotype appear to be interesting for our objectives.
{"title":"EVALUATION OF DIFFERENT GERMPLASM OF BRASSICA JUNCEA AGAINST OROBANCHE AEGYPTIACA","authors":"G. Akhter, T. A. Khan","doi":"10.25081/RIPB.2017.V7.3604","DOIUrl":"https://doi.org/10.25081/RIPB.2017.V7.3604","url":null,"abstract":"Among the major weed groups that cause huge economic losses to important cropping systems, Orobanche species are greatly devastating. Egyptian broomrape (Orobanche aegyptiaca) is a parasitic weed causing major yield loss in many field and vegetable crops and is a serious threat to Indian mustard. In this study, severalgenotypes of Indian mustard were screened in order to identify resistant genotypes against O. aegyptiaca. In the greenhouse conditions, genotypes were different in the degree of susceptibility to Broomrape. Attachment number, emergence number, and dry matter of parasitic broomrape were affected by biomass of genotype. A significant impact of the parasitism onto the dry weight of all infected mustard genotype with variable degree was observed. Broomrape attachment was observed in all the cultivated genotypes with Pusa mustard 24 being the most susceptible with the greatest number of emerged Orobanche shoots. In contrast, no emergence shoots were observed in four out of the fifteen genotype viz., Pusa Jaikisan, Pusa bold, Pusa Vijay and Pusa mustard 26 which have less attachment number and emergence number. These genotype appear to be interesting for our objectives.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122303682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-25DOI: 10.19071/RIPB.2017.V7.3310
Geetha Menon, G. T. Dabhade
The present study reports ten taxa of leafy hepatics (Jugermanniae) viz. Lejeunea cavifolia, L. flava ;Taxilejeunea indica ; Cololejeunea lanciloba, C.himalayensis; Ptycanthus striatus; Frullania ericoides; Jungermannia tetragona; Notoscyphus pandeii and Plagiochilla chopraii from the ranges of Western Ghats lying in Maharashtra.These ten taxa belonged to eight genera under four families.The descriptions of each specimen, illustrations and localities of collection are presented. Among these Cololejeunea lanciloba , C.himalayensis are epiphyllous, J.tetragona is terricolous, while rest of them are corticolous.
{"title":"LEAFY HEPATICS FROM SOME REGIONS OF WESTERN GHATS (MAHARASHTRA)","authors":"Geetha Menon, G. T. Dabhade","doi":"10.19071/RIPB.2017.V7.3310","DOIUrl":"https://doi.org/10.19071/RIPB.2017.V7.3310","url":null,"abstract":"The present study reports ten taxa of leafy hepatics (Jugermanniae) viz. Lejeunea cavifolia, L. flava ;Taxilejeunea indica ; Cololejeunea lanciloba, C.himalayensis; Ptycanthus striatus; Frullania ericoides; Jungermannia tetragona; Notoscyphus pandeii and Plagiochilla chopraii from the ranges of Western Ghats lying in Maharashtra.These ten taxa belonged to eight genera under four families.The descriptions of each specimen, illustrations and localities of collection are presented. Among these Cololejeunea lanciloba , C.himalayensis are epiphyllous, J.tetragona is terricolous, while rest of them are corticolous.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124836700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-04DOI: 10.19071/RIPB.2017.V7.3248
P. Santosh, S. B. Arakera
Annatto is one of the important natural food colourant widely used in dairy industry. Quality RNA in large quantity is often required in the analysis of gene expression. RNA extraction from samples collected from woody plants is generally complex, and becomes the main limitation to study gene expression, particularly in crops like Bixa orellana . Standard RNA extraction protocols are time consuming, laborious and cannot be adapted for high throughput functional analysis. Therefore a simple and effective protocol for extraction of high quality total RNA from bark tissue of woody stem was achieved using the RNeasy plant mini kit (Qiagen, USA). The extracted RNA was successfully converted into double-stranded cDNA using the SMATer cDNA synthesis kit (Clontech, USA) which is based on the S witching M echanism A t 5’ End of R NA T ranscript (SMART) technology. The integrity of the total RNA used for synthesizing double stranded cDNA was assessed agarose gel electrophoresis and by PCR. As expected, the PCR product contained the full coding sequence plus 69 and 196 bp of 5’ and 3’ UTRs respectively. The double-stranded cDNA was used successfully for creating a SSH cDNA library. The cDNA could also be useful for a number of other applications like cDNA library construction, EST analysis, RACE and Next Generation Sequencing (NGS).
{"title":"Total RNA Isolation and cDNA synthesis from Bixa orellana bark","authors":"P. Santosh, S. B. Arakera","doi":"10.19071/RIPB.2017.V7.3248","DOIUrl":"https://doi.org/10.19071/RIPB.2017.V7.3248","url":null,"abstract":"Annatto is one of the important natural food colourant widely used in dairy industry. Quality RNA in large quantity is often required in the analysis of gene expression. RNA extraction from samples collected from woody plants is generally complex, and becomes the main limitation to study gene expression, particularly in crops like Bixa orellana . Standard RNA extraction protocols are time consuming, laborious and cannot be adapted for high throughput functional analysis. Therefore a simple and effective protocol for extraction of high quality total RNA from bark tissue of woody stem was achieved using the RNeasy plant mini kit (Qiagen, USA). The extracted RNA was successfully converted into double-stranded cDNA using the SMATer cDNA synthesis kit (Clontech, USA) which is based on the S witching M echanism A t 5’ End of R NA T ranscript (SMART) technology. The integrity of the total RNA used for synthesizing double stranded cDNA was assessed agarose gel electrophoresis and by PCR. As expected, the PCR product contained the full coding sequence plus 69 and 196 bp of 5’ and 3’ UTRs respectively. The double-stranded cDNA was used successfully for creating a SSH cDNA library. The cDNA could also be useful for a number of other applications like cDNA library construction, EST analysis, RACE and Next Generation Sequencing (NGS).","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133553751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-19DOI: 10.19071/ripb.2016.v6.3141
F. Ismail, M. Malahubban, Mohammad Hailmi Sajili, Zakry Fitri Ab Aziz
The present study was conducted to isolate and characterize endophytic bacteria isolated from root nodules of Acacia mangium Willd and subsequently tested for plant growth promotion. A total of five bacterial isolates were successfully isolated and subjected to morphological and biochemical examination. The present study found that all isolates had almost similar morphologically but differed in growth rate. All isolates were negative on N-free and IAA tests, though positive on P solubilisation test. The present study demonstrated that the inoculation with isolate FSI3 had significantly improved (p 0.05) root and shoot length of winged bean seedlings. The present study suggests that the stimulatory effect by isolate FSI3 may be associated with P-solubilizing ability. A further test on isolate FSI3 is considered essential in future to uncover the several other possible plant growth-promoting mechanisms.
{"title":"Plant growth-promoting properties of cultivable endophytic root nodule bacterial isolates from Acacia mangium Willd","authors":"F. Ismail, M. Malahubban, Mohammad Hailmi Sajili, Zakry Fitri Ab Aziz","doi":"10.19071/ripb.2016.v6.3141","DOIUrl":"https://doi.org/10.19071/ripb.2016.v6.3141","url":null,"abstract":"The present study was conducted to isolate and characterize endophytic bacteria isolated from root nodules of Acacia mangium Willd and subsequently tested for plant growth promotion. A total of five bacterial isolates were successfully isolated and subjected to morphological and biochemical examination. The present study found that all isolates had almost similar morphologically but differed in growth rate. All isolates were negative on N-free and IAA tests, though positive on P solubilisation test. The present study demonstrated that the inoculation with isolate FSI3 had significantly improved (p 0.05) root and shoot length of winged bean seedlings. The present study suggests that the stimulatory effect by isolate FSI3 may be associated with P-solubilizing ability. A further test on isolate FSI3 is considered essential in future to uncover the several other possible plant growth-promoting mechanisms.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133996116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this research, callus from leaves, petioles and stems of Achyranthes bidentata was evidently initiated by plant growth substance, in which 2,4-dichlorophenoxyacetic acid (2,4-D) was very important to callus induction, but effects of other plant growth substances were various, and the optimum combination of plant growth substances for callus induction from leaves, petioles and stems was respectively obtained. Compared with callus induction from leaves and petioles, callus induction from stems was easier, and the higher induction rate and bigger mass of callus from stems were obtained. This study showed that the dedifferentiation capacity of various explants from Achyranthes bidentata was obviously different, and effects of plant growth substance on callus induction from various explants of Achyranthes bidentata were significantly diverse.
{"title":"Roles of plant growth substance in callus induction of Achyranthes bidentata","authors":"H. Duan, Wei Ding, Jianying Song, Jiaming Xu, Huina Wang, Yanqiu Zhu, Wenxiao Liu, Yanqing Zhou","doi":"10.19071/RIPB.2016.V6.3079","DOIUrl":"https://doi.org/10.19071/RIPB.2016.V6.3079","url":null,"abstract":"In this research, callus from leaves, petioles and stems of Achyranthes bidentata was evidently initiated by plant growth substance, in which 2,4-dichlorophenoxyacetic acid (2,4-D) was very important to callus induction, but effects of other plant growth substances were various, and the optimum combination of plant growth substances for callus induction from leaves, petioles and stems was respectively obtained. Compared with callus induction from leaves and petioles, callus induction from stems was easier, and the higher induction rate and bigger mass of callus from stems were obtained. This study showed that the dedifferentiation capacity of various explants from Achyranthes bidentata was obviously different, and effects of plant growth substance on callus induction from various explants of Achyranthes bidentata were significantly diverse.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129862918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：