Yuanyuan Zhang, Jiaren Yan, Liping Song, Yongan Zhu, Peng Xu, S. Mao, Bin Hu, Jun Wu, Bingli Wang
Antibacterial properties of curcumin from turmeric (Curcuma longa) and enrofloxacin against Aeromonas hydrophila were assayed. The minimum inhibitory concentration (MIC) values of curcumin and enrofloxacin against A. hydrophila were found to be 100ug/ml and 9.375ug/ml, respectively. To realize the mechanisms of action of curcumin against A. hydrophila, we researched the antibacterial activity and bacterial membrane permeability of A. hydrophila cells treated with curcumin or enrofloxacin. All results elucidated that curcumin increased membrane permeabilization and caused leakage of intracellular contents, while its role was not as good as enrofloxacin. Moreover, a synergistic effect was shown between curcumin and enrofloxacin. The present study suggests that curcumin extracted from turmeric has the potential to be used as an antimicrobial for the control of A. hydrophila.
{"title":"Comparison study of antibacterial properties of curcumin from Curcuma longa and enrofloxacin against Aeromonas hydrophila","authors":"Yuanyuan Zhang, Jiaren Yan, Liping Song, Yongan Zhu, Peng Xu, S. Mao, Bin Hu, Jun Wu, Bingli Wang","doi":"10.46989/001c.38916","DOIUrl":"https://doi.org/10.46989/001c.38916","url":null,"abstract":"Antibacterial properties of curcumin from turmeric (Curcuma longa) and enrofloxacin against Aeromonas hydrophila were assayed. The minimum inhibitory concentration (MIC) values of curcumin and enrofloxacin against A. hydrophila were found to be 100ug/ml and 9.375ug/ml, respectively. To realize the mechanisms of action of curcumin against A. hydrophila, we researched the antibacterial activity and bacterial membrane permeability of A. hydrophila cells treated with curcumin or enrofloxacin. All results elucidated that curcumin increased membrane permeabilization and caused leakage of intracellular contents, while its role was not as good as enrofloxacin. Moreover, a synergistic effect was shown between curcumin and enrofloxacin. The present study suggests that curcumin extracted from turmeric has the potential to be used as an antimicrobial for the control of A. hydrophila.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79709334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. J. Apines-Amar, Donna C. Rendaje, Jesel F. Cadangin, L. Piňosa, Jean Rose H. Maquirang, Fiona L. Pedroso, L. Laureta
Hatchery seed production of mussels has been identified as a sustainable method to address the problem of low production due to insufficient seed supply. We conducted the first hatchery production trials of the Asian green mussel Perna viridis spats in the Philippines to demonstrate the feasibility of producing a sufficient and reliable seed supply for grow-out operations. However, results of small-scale experiments cannot be directly rolled-out commercially unless these are verified in bigger-scale facilities approximating those of commercial hatcheries. Thus, techniques on broodstock collection, spawning, and larval rearing, developed by the project in the laboratory during the experimental trials, were applied and verified in a production run using industry-scale tank facilities. Mature broodstocks collected from traditional mussel growing areas were successfully spawned in the hatchery. Eggs were fertilized, and these developed into D-hinged larvae, pediveliger, and metamorphosed into early spat before fully developing into the spat stage. Successful larval rearing up to the spat stage required the use of appropriate algal species and rates of feeding, close monitoring of larval stages, and water quality management. The survival from eggs to D-hinged larvae, D-hinged larvae to pediveliger, pediveliger to early spat, D-hinged larvae to early spat, and early spat (1 mm) to fully grown spat was 77, 64, 6.4, 3.1, and 72%, respectively. This study has established the feasibility of producing P. viridis in commercial-scale hatchery facilities.
{"title":"Verification of a hatchery protocol for green mussel Perna viridis spat production in the Philippines using industry-scale facilities","authors":"M. J. Apines-Amar, Donna C. Rendaje, Jesel F. Cadangin, L. Piňosa, Jean Rose H. Maquirang, Fiona L. Pedroso, L. Laureta","doi":"10.46989/001c.38906","DOIUrl":"https://doi.org/10.46989/001c.38906","url":null,"abstract":"Hatchery seed production of mussels has been identified as a sustainable method to address the problem of low production due to insufficient seed supply. We conducted the first hatchery production trials of the Asian green mussel Perna viridis spats in the Philippines to demonstrate the feasibility of producing a sufficient and reliable seed supply for grow-out operations. However, results of small-scale experiments cannot be directly rolled-out commercially unless these are verified in bigger-scale facilities approximating those of commercial hatcheries. Thus, techniques on broodstock collection, spawning, and larval rearing, developed by the project in the laboratory during the experimental trials, were applied and verified in a production run using industry-scale tank facilities. Mature broodstocks collected from traditional mussel growing areas were successfully spawned in the hatchery. Eggs were fertilized, and these developed into D-hinged larvae, pediveliger, and metamorphosed into early spat before fully developing into the spat stage. Successful larval rearing up to the spat stage required the use of appropriate algal species and rates of feeding, close monitoring of larval stages, and water quality management. The survival from eggs to D-hinged larvae, D-hinged larvae to pediveliger, pediveliger to early spat, D-hinged larvae to early spat, and early spat (1 mm) to fully grown spat was 77, 64, 6.4, 3.1, and 72%, respectively. This study has established the feasibility of producing P. viridis in commercial-scale hatchery facilities.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90725986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Wan, Shunyao Jiang, Qinxue Xiong, Jun R. Yang, Sujing Xia, Qihang Chen
Thermal stratification and mixing in aquaculture ponds can seriously affect the vertical distribution of dissolved oxygen (DO) and result in a lack of DO in ponds. Therefore, revealing the vertical distribution of DO in aquaculture ponds influenced by thermal stratification and mixing can provide a theoretical basis for aquaculture water management. Here, we explore the impacts of thermal stratification and mixing on the vertical distribution of DO in two aquaculture ponds under different weather conditions. The results showed that thermal stratification mainly occurs during the daytime, and mixing occurs at nighttime. Water thermal stratification appears 4 h after sunrise, while mixing occurs 1 h after nightfall. When the Richardson index is less than 0.25, the mixing direction is unstable. In the daytime, the vertical distribution of DO, chlorophyll a (Chl-a), and phytoplankton abundance varied with thermal stratification and mixing. The concentration of DO gradually dropped with increasing water depth during the nighttime. The concentration of DO was lowest in the early morning and peaked in the afternoon. Multiple regression analysis demonstrated that water temperature (WT), Chl-a, and phytoplankton abundance provided the best model for the vertical distribution of DO. Based on our results, DO regulation can provide important insights for aquaculture pond management.
{"title":"Effects of thermal stratification and mixing on the vertical distribution of dissolved oxygen in aquaculture ponds","authors":"Xiao Wan, Shunyao Jiang, Qinxue Xiong, Jun R. Yang, Sujing Xia, Qihang Chen","doi":"10.46989/001c.38406","DOIUrl":"https://doi.org/10.46989/001c.38406","url":null,"abstract":"Thermal stratification and mixing in aquaculture ponds can seriously affect the vertical distribution of dissolved oxygen (DO) and result in a lack of DO in ponds. Therefore, revealing the vertical distribution of DO in aquaculture ponds influenced by thermal stratification and mixing can provide a theoretical basis for aquaculture water management. Here, we explore the impacts of thermal stratification and mixing on the vertical distribution of DO in two aquaculture ponds under different weather conditions. The results showed that thermal stratification mainly occurs during the daytime, and mixing occurs at nighttime. Water thermal stratification appears 4 h after sunrise, while mixing occurs 1 h after nightfall. When the Richardson index is less than 0.25, the mixing direction is unstable. In the daytime, the vertical distribution of DO, chlorophyll a (Chl-a), and phytoplankton abundance varied with thermal stratification and mixing. The concentration of DO gradually dropped with increasing water depth during the nighttime. The concentration of DO was lowest in the early morning and peaked in the afternoon. Multiple regression analysis demonstrated that water temperature (WT), Chl-a, and phytoplankton abundance provided the best model for the vertical distribution of DO. Based on our results, DO regulation can provide important insights for aquaculture pond management.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81754206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Supriyono, D. Soelistyowati, K. Adiyana, L. Thesiana
Spiny lobsters (Panulirus sp.) were valuable and one of the most popular Indonesian export commodities. Some approaches were made to increase the quantity and quality of cultivated spiny lobsters. Land-based mariculture with Recirculating Aquaculture System (RAS) was applied to increase lobster harvesting and optimize environmental quality by adjusting water alkalinity. This study aimed to determine the optimum level of alkalinity for spiny lobsters Panulirus homarus rearing in RAS. This study investigated the effects of applying four water alkalinity levels (Control, 125, 200, and 275 mg L-1 CaCO3) on the biochemical responses of P. homarus observed in the hemolymph in terms of Total Hemocyte Count (THC), glucose, total protein, calcium, and pH levels. Furthermore, we also studied the alkalinity effects on lobster production performance parameters in terms of body weight gain, body length, Survival Rate (SR), Specific Growth Rate (SGR), and Feed Conversion Ratio (FCR). Lobsters with an initial weight rate of 58.05±1.69 g and an initial total length rate of 115.33±1.52 mm were reared for 60 days in a recirculation system. Results of water quality parameters such as ammonia, nitrite, nitrate, dissolved oxygen, temperature, and salinity during the study were available for lobster rearing. Different alkalinity levels affected the biochemical responses and production performance of lobsters. The best alkalinity level to reared Panulirus sp. in the recirculation system during this study was 200 mg L-1 CaCO3 so that it could achieve the highest survival rate of 86.67% with SGR 0.60±0.01 % day-1.
{"title":"The effects of alkalinity on production performance and biochemical responses of spiny lobster Panulirus homarus reared in recirculating aquaculture system","authors":"E. Supriyono, D. Soelistyowati, K. Adiyana, L. Thesiana","doi":"10.46989/001c.38426","DOIUrl":"https://doi.org/10.46989/001c.38426","url":null,"abstract":"Spiny lobsters (Panulirus sp.) were valuable and one of the most popular Indonesian export commodities. Some approaches were made to increase the quantity and quality of cultivated spiny lobsters. Land-based mariculture with Recirculating Aquaculture System (RAS) was applied to increase lobster harvesting and optimize environmental quality by adjusting water alkalinity. This study aimed to determine the optimum level of alkalinity for spiny lobsters Panulirus homarus rearing in RAS. This study investigated the effects of applying four water alkalinity levels (Control, 125, 200, and 275 mg L-1 CaCO3) on the biochemical responses of P. homarus observed in the hemolymph in terms of Total Hemocyte Count (THC), glucose, total protein, calcium, and pH levels. Furthermore, we also studied the alkalinity effects on lobster production performance parameters in terms of body weight gain, body length, Survival Rate (SR), Specific Growth Rate (SGR), and Feed Conversion Ratio (FCR). Lobsters with an initial weight rate of 58.05±1.69 g and an initial total length rate of 115.33±1.52 mm were reared for 60 days in a recirculation system. Results of water quality parameters such as ammonia, nitrite, nitrate, dissolved oxygen, temperature, and salinity during the study were available for lobster rearing. Different alkalinity levels affected the biochemical responses and production performance of lobsters. The best alkalinity level to reared Panulirus sp. in the recirculation system during this study was 200 mg L-1 CaCO3 so that it could achieve the highest survival rate of 86.67% with SGR 0.60±0.01 % day-1.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80967052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of dietary supplementation of DL-alpha-tocopheryl acetate (a-TA) on growth performance, feed efficiency, survival, and salinity resistance of Asian catfish (Clarias macrocephalus) fry was evaluated. A total of 750 catfish fry were divided into five treatments with three replicates and fed experimental diets containing varying levels of alpha-TA: 0, 100, 200, 300, and 400 mg·kg-1 diet for 45 days. Results show that catfish fry-fed diets supplemented with alpha-TA exhibited significantly higher weight gain (WG) and final average body weight (FABW), better specific growth rates (SGR) and feed conversion ratio (FCR), and significantly higher survival rates compared to those of the control group fed the basal diet (P < 0.05). Better salinity resistance was observed in the Asian catfish fed the alpha-TA-supplemented diets when abruptly exposed to higher salinity (10 ppt) for up to 48 h. In addition, the diet attractability test revealed that alpha-TA-supplemented diets attracted significantly more catfish fry than the basal diet lacking vitamin E (P < 0.05). In conclusion, the results demonstrated that dietary supplementation of alpha-TA (vitamin E) improved growth, feed efficiency, feed attractability, survival, and resistance to abrupt higher salinity exposure of Clarias macrocephalus fry.
{"title":"Dietary DL-alpha-tocopheryl acetate enhances growth, survival, and resistance against an abrupt shift to higher salinity of the Asian catfish (Clarias macrocephalus) fry","authors":"Lemark M. Bautista, B. Tumbokon, A. E. Serrano","doi":"10.46989/001c.38249","DOIUrl":"https://doi.org/10.46989/001c.38249","url":null,"abstract":"The effect of dietary supplementation of DL-alpha-tocopheryl acetate (a-TA) on growth performance, feed efficiency, survival, and salinity resistance of Asian catfish (Clarias macrocephalus) fry was evaluated. A total of 750 catfish fry were divided into five treatments with three replicates and fed experimental diets containing varying levels of alpha-TA: 0, 100, 200, 300, and 400 mg·kg-1 diet for 45 days. Results show that catfish fry-fed diets supplemented with alpha-TA exhibited significantly higher weight gain (WG) and final average body weight (FABW), better specific growth rates (SGR) and feed conversion ratio (FCR), and significantly higher survival rates compared to those of the control group fed the basal diet (P < 0.05). Better salinity resistance was observed in the Asian catfish fed the alpha-TA-supplemented diets when abruptly exposed to higher salinity (10 ppt) for up to 48 h. In addition, the diet attractability test revealed that alpha-TA-supplemented diets attracted significantly more catfish fry than the basal diet lacking vitamin E (P < 0.05). In conclusion, the results demonstrated that dietary supplementation of alpha-TA (vitamin E) improved growth, feed efficiency, feed attractability, survival, and resistance to abrupt higher salinity exposure of Clarias macrocephalus fry.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77409563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yundong Li, F. Zhou, Qi-bin Yang, Song Jiang, Li-Shi Yang, Jianhua Huang, Xinfa Chen, Qianlin Mai, Shi-gui Jiang
A novel TRY homolog was cloned in Penaeus monodon by RACE technology, named PmTry (GenBank: KP998480). The PmTry cDNA was 916 bp, which encodes 266 amino acids with a predicted molecular weight of 28.38 KDa and an isoelectric point of 4.58. Homologous analysis indicated that PmTry shared 42%~91% similarity with other species. The phylogenetic tree showed that PmTry was closely related to Fenneropenaeus chinensis. Tissue expression profiles showed that PmTry was highest expressed in the hepatopancreas and the lowest expressed in the eyestalk nerve. It was expressed in the whole growth stage of P. monodon, but the relative expression level of each stage was significantly different. In addition, PmTry-524 and PmTry-798 were particularly related to growth traits of P. monodon by genotype. The SNP markers may provide a basis for genetic selection and breed improvement studies in P. monodon.
{"title":"Molecular cloning, expression pattern of Trypsin gene and association analysis with growth traits in Penaeus monodon","authors":"Yundong Li, F. Zhou, Qi-bin Yang, Song Jiang, Li-Shi Yang, Jianhua Huang, Xinfa Chen, Qianlin Mai, Shi-gui Jiang","doi":"10.46989/001c.38101","DOIUrl":"https://doi.org/10.46989/001c.38101","url":null,"abstract":"A novel TRY homolog was cloned in Penaeus monodon by RACE technology, named PmTry (GenBank: KP998480). The PmTry cDNA was 916 bp, which encodes 266 amino acids with a predicted molecular weight of 28.38 KDa and an isoelectric point of 4.58. Homologous analysis indicated that PmTry shared 42%~91% similarity with other species. The phylogenetic tree showed that PmTry was closely related to Fenneropenaeus chinensis. Tissue expression profiles showed that PmTry was highest expressed in the hepatopancreas and the lowest expressed in the eyestalk nerve. It was expressed in the whole growth stage of P. monodon, but the relative expression level of each stage was significantly different. In addition, PmTry-524 and PmTry-798 were particularly related to growth traits of P. monodon by genotype. The SNP markers may provide a basis for genetic selection and breed improvement studies in P. monodon.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91111294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qingqing Jiang, Zhuming Zhao, Y. Mu, Dan Zhao, Biao Wu
Scallops are economically important shellfish widely cultured in China. In this study, the agglomeration characteristics of the scallop aquaculture industry in Chinese coastal provinces were explored based on data from the China Fishery Statistical Yearbook from 2012 to 2020 and the comparative advantage and concentration level of the scallop breeding industry. The data were analyzed using the comprehensive relative advantage index, location entropy, and industrial concentration degree method. Two main results revealed that Hebei Province had the highest comparative advantage in terms of efficiency, followed by Fujian, Guangdong, Shandong, Guangxi, Liaoning, and Zhejiang Provinces. Hebei Province had the highest comparative advantage in scale, followed by Liaoning, Shandong, Hainan, Guangdong, Guangxi, Fujian, and Zhejiang Provinces. Additionally, the scallop cultivation industry in Hebei, Shandong, and Liaoning Provinces showed a high concentration, and the proportion of scallop production in the national production (CR4) in the three provinces, as well as in Guangdong, accounted for more than 99% year-round. Overall, Hebei, Shandong, and Liaoning Provinces had a comparative advantage in scale, comprehensive comparative advantage, and industrial agglomeration.
{"title":"A study on the agglomeration characteristics of the scallop aquaculture industry in China","authors":"Qingqing Jiang, Zhuming Zhao, Y. Mu, Dan Zhao, Biao Wu","doi":"10.46989/001c.38068","DOIUrl":"https://doi.org/10.46989/001c.38068","url":null,"abstract":"Scallops are economically important shellfish widely cultured in China. In this study, the agglomeration characteristics of the scallop aquaculture industry in Chinese coastal provinces were explored based on data from the China Fishery Statistical Yearbook from 2012 to 2020 and the comparative advantage and concentration level of the scallop breeding industry. The data were analyzed using the comprehensive relative advantage index, location entropy, and industrial concentration degree method. Two main results revealed that Hebei Province had the highest comparative advantage in terms of efficiency, followed by Fujian, Guangdong, Shandong, Guangxi, Liaoning, and Zhejiang Provinces. Hebei Province had the highest comparative advantage in scale, followed by Liaoning, Shandong, Hainan, Guangdong, Guangxi, Fujian, and Zhejiang Provinces. Additionally, the scallop cultivation industry in Hebei, Shandong, and Liaoning Provinces showed a high concentration, and the proportion of scallop production in the national production (CR4) in the three provinces, as well as in Guangdong, accounted for more than 99% year-round. Overall, Hebei, Shandong, and Liaoning Provinces had a comparative advantage in scale, comprehensive comparative advantage, and industrial agglomeration.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84428596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanming Sui, Jiyi Chen, Weiwei Ji, Wen Ruan, Yanyan Xu, Liang Zheng, Yanyan Lu
We assessed the expressions of MHCI, LYZC, keratin8, MPO, DUSP1, IκBα, Rab21, and Rac2 between two species of carps (Erqisi river crucian carp and allogynogenetic crucian carp) after Cyprinid herpesvirus 2 (CyHV-2) infection. The relative expressions of MHCI, LYZC, and keratin8 in the virus-challenged groups were significantly higher than control groups. Moreover, the expression of IκBα in the virus-challenged groups was significantly lower than in the control groups. Compared with the virus-challenged ERO group, the expression of IκBα in the virus-challenged ZHO group decreased. The expression of Rab21 in the virus-challenged groups gradually increased and was significantly higher than in the control groups, and then its expression began to decrease after 24 h. At 72 h, the expression of IκBα in both virus-challenged groups was significantly lower than in the control groups. In addition, the expression of Rab21 in the virus-challenged ZHO group was significantly higher than the virus-challenged ERO group at all time points except for 72 h. Before 24 h, the expression of Rac2 remained unchanged in these four groups, and its expression in the virus-challenged ZHO group was significantly higher than in the other three groups. Nevertheless, its expression began to decrease after 24 h but was still slightly higher than the control group at 72 h. MPO showed a similar expression pattern as Rac2. The expression of DUSP1 in the four groups was the same at 0 h. However, its expression in the virus-challenged ZHO group was significantly higher than in the other three groups at other time points.
{"title":"Comparative analysis of differential gene expression in two species of crucian carps in response to Cyprinid herpesvirus 2 (CyHV-2) infection","authors":"Yanming Sui, Jiyi Chen, Weiwei Ji, Wen Ruan, Yanyan Xu, Liang Zheng, Yanyan Lu","doi":"10.46989/001c.38010","DOIUrl":"https://doi.org/10.46989/001c.38010","url":null,"abstract":"We assessed the expressions of MHCI, LYZC, keratin8, MPO, DUSP1, IκBα, Rab21, and Rac2 between two species of carps (Erqisi river crucian carp and allogynogenetic crucian carp) after Cyprinid herpesvirus 2 (CyHV-2) infection. The relative expressions of MHCI, LYZC, and keratin8 in the virus-challenged groups were significantly higher than control groups. Moreover, the expression of IκBα in the virus-challenged groups was significantly lower than in the control groups. Compared with the virus-challenged ERO group, the expression of IκBα in the virus-challenged ZHO group decreased. The expression of Rab21 in the virus-challenged groups gradually increased and was significantly higher than in the control groups, and then its expression began to decrease after 24 h. At 72 h, the expression of IκBα in both virus-challenged groups was significantly lower than in the control groups. In addition, the expression of Rab21 in the virus-challenged ZHO group was significantly higher than the virus-challenged ERO group at all time points except for 72 h. Before 24 h, the expression of Rac2 remained unchanged in these four groups, and its expression in the virus-challenged ZHO group was significantly higher than in the other three groups. Nevertheless, its expression began to decrease after 24 h but was still slightly higher than the control group at 72 h. MPO showed a similar expression pattern as Rac2. The expression of DUSP1 in the four groups was the same at 0 h. However, its expression in the virus-challenged ZHO group was significantly higher than in the other three groups at other time points.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73651622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catfish are important organisms used extensively in aquaculture. For this review, we are focusing on specific species of catfish in the genus Clarias, primarily Clarias batrachus, C. gariepinus and C. macrocephalus, because of their widespread use in aquaculture and the considerable amount of information that has been amassed on different aspects of their biology. This review summarizes information available on topics ranging from the taxonomy and geographic distribution of these catfish to work done on them at the genetic, molecular biological and genomic levels. This information may be useful in future efforts to grow and expand their use in aquaculture.
鲶鱼是广泛应用于水产养殖的重要生物。由于鲶鱼在水产养殖中的广泛应用以及在其生物学的各个方面积累了大量的信息，因此本文将重点介绍Clarias属的特定种类，主要是Clarias batrachus, C. gariepinus和C. macrocephalus。本文综述了从鲶鱼的分类和地理分布到在遗传、分子生物学和基因组水平上所做的工作等方面的信息。这一信息可能对今后努力增加和扩大其在水产养殖中的使用有用。
{"title":"Biology of selected Clarias catfish species used in aquaculture","authors":"D. Haymer, G. Khedkar","doi":"10.46989/001c.37958","DOIUrl":"https://doi.org/10.46989/001c.37958","url":null,"abstract":"Catfish are important organisms used extensively in aquaculture. For this review, we are focusing on specific species of catfish in the genus Clarias, primarily Clarias batrachus, C. gariepinus and C. macrocephalus, because of their widespread use in aquaculture and the considerable amount of information that has been amassed on different aspects of their biology. This review summarizes information available on topics ranging from the taxonomy and geographic distribution of these catfish to work done on them at the genetic, molecular biological and genomic levels. This information may be useful in future efforts to grow and expand their use in aquaculture.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84158851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Nugroho, Riza Zulkarnain, A. Kristanto, R. R. Dewi, B. Gunadi, A. Robisalmi, W. Pamungkas, L. Setijaningsih, M. Rifaldi
The decreasing productivity and increasing environmental pressure on intensive tilapia culture in open waters, especially in floating net cages, have forced the development of a biofloc system for tilapia farming. This study aimed to develop the superior tilapia strain suitable for the biofloc system and to observe their productivity and economic profitability under commercial scale in the biofloc system. The selective breeding activity was carried out on 100 families reared and mated in hapa (a double-walled contrivance in which an inner hapa, made of mosquito netting material, is installed within an outer one made of whole cloth) in the biofloc pond for three generations. The individual selection was conducted at the age of 6 months on the characters of standard length and body weight with the best 10% of the top part of the population as a reference for the formation of the next generation. After three generations, the offspring obtained from mass spawning were grown out in round-limen ponds and square-concrete ponds for productivity and profitability observations. The results showed that heritability values of the biofloc tilapia strain were 0.4196 (standard length, SL) and 0.4898 (body weight, BW). The average response selection of BW and SL was 13.04% and 6.11%, respectively. Biomass gain was obtained between 19.29–28.83 kg/m3. The production multiply value was 8.07-13.59x. The average daily weight growth was between 2.8g-3.0g. The Benefit-Cost ratio is valued from 1.81 to 2.71. The superior tilapia biofloc strain has a moderate heritability value. When it is raised in a biofloc ponds system at different volumes, it significantly affects the productivity on biomass, body weight, and multiplication value. Also, it is advantageous when applied on an industrial scale.
{"title":"Assembling the superior biofloc tilapia strains for industry","authors":"E. Nugroho, Riza Zulkarnain, A. Kristanto, R. R. Dewi, B. Gunadi, A. Robisalmi, W. Pamungkas, L. Setijaningsih, M. Rifaldi","doi":"10.46989/001c.37895","DOIUrl":"https://doi.org/10.46989/001c.37895","url":null,"abstract":"The decreasing productivity and increasing environmental pressure on intensive tilapia culture in open waters, especially in floating net cages, have forced the development of a biofloc system for tilapia farming. This study aimed to develop the superior tilapia strain suitable for the biofloc system and to observe their productivity and economic profitability under commercial scale in the biofloc system. The selective breeding activity was carried out on 100 families reared and mated in hapa (a double-walled contrivance in which an inner hapa, made of mosquito netting material, is installed within an outer one made of whole cloth) in the biofloc pond for three generations. The individual selection was conducted at the age of 6 months on the characters of standard length and body weight with the best 10% of the top part of the population as a reference for the formation of the next generation. After three generations, the offspring obtained from mass spawning were grown out in round-limen ponds and square-concrete ponds for productivity and profitability observations. The results showed that heritability values of the biofloc tilapia strain were 0.4196 (standard length, SL) and 0.4898 (body weight, BW). The average response selection of BW and SL was 13.04% and 6.11%, respectively. Biomass gain was obtained between 19.29–28.83 kg/m3. The production multiply value was 8.07-13.59x. The average daily weight growth was between 2.8g-3.0g. The Benefit-Cost ratio is valued from 1.81 to 2.71. The superior tilapia biofloc strain has a moderate heritability value. When it is raised in a biofloc ponds system at different volumes, it significantly affects the productivity on biomass, body weight, and multiplication value. Also, it is advantageous when applied on an industrial scale.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83967743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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