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Journal of Apiproduct and Apimedical Science最新文献

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Absence of propolis effect on plasma glycaemic control and lipid metabolism in a diabetic rat model. 蜂胶对糖尿病大鼠模型血糖控制及脂质代谢的影响。
Pub Date : 2009-04-01 DOI: 10.3896/IBRA.4.01.2.05
J. M. Sforcin
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引用次数: 8
Composition and antioxidant activity of propolis from three species of scaptotrigona stingless bees 三种无刺蜂蜂胶的组成及抗氧化活性
Pub Date : 2009-04-01 DOI: 10.3896/IBRA.4.01.2.03
A. Sawaya, J. C. P. Calado, L. Santos, M. C. Marcucci, I. P. Akatsu, A. Soares, P. Abdelnur, I. B. S. Cunha, M. Eberlin
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引用次数: 51
The effect of propolis on pro-inflammatory cytokines produced by melanoma-bearing mice submitted to chronic stress. 蜂胶对慢性应激下黑色素瘤小鼠产生的促炎细胞因子的影响。
Pub Date : 2009-02-10 DOI: 10.3896/IBRA.4.01.1.05
J. M. Sforcin
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引用次数: 22
Honey modulates biofilms of Pseudomonas aeruginosain a time and dose dependent manner. 蜂蜜对铜绿假单胞菌生物膜的调节具有时间和剂量依赖性。
Pub Date : 2009-02-10 DOI: 10.3896/IBRA.4.01.1.03
R. Cooper
Biofilms are complex microbial communities associated with persistent infections that demonstrate increased resistance to immunological and antimicrobial challenges. Pseudomonas aeruginosa has been associated with biofilms in chronic wounds. When this opportunist pathogen is grown in suspension culture in the laboratory it is susceptible to manuka honey at concentrations below 10 % (v/v), but its susceptibility as a biofilm has not previously been reported. The effect of two concentrations of manuka honey dissolved in Luria broth (LB) on biofilms of six cultures of Pseudomonas aeruginosa were tested over 24 hours. One type culture (ATCC 27853) and five clinical isolates derived from five different patients with infected wounds were each incubated at 37 oC for 24 hours in LB in microtitre plates to allow biofilms to establish. Medium was then replaced with LB, or LB containing 20 % (w/v) manuka honey or LB containing 40 % (w/v) manuka honey and plates were incubated at 37 oC for 24 hours. Biofilm biomass was monitored at known intervals by fixing adherent cells with 2.5 % (w/v) glutaraldehyde and staining with 0.25 % (w/v) crystal violet. Exposure of Pseudomonas aeruginosa biofilms to 40 % (w/v) manuka honey in LB resulted in significantly reduced biofilm biomass for all cultures (p >0.05) compared to LB alone and 20 % (w/v) manuka honey in LB. Differences in biofilm biomass were most noticeable after 9 to 11 hour exposure times. This preliminary investigation suggests that manuka honey has potential in the control of biofilms in chronic wounds and justifies further study of this subject.
生物膜是复杂的微生物群落,与持续感染有关,表现出对免疫和抗微生物挑战的抵抗力增强。铜绿假单胞菌与慢性伤口的生物膜有关。当这种机会主义病原体在实验室悬浮培养中生长时,它对浓度低于10% (v/v)的麦卢卡蜂蜜敏感,但其作为生物膜的敏感性此前尚未报道。研究了两种浓度的麦卢卡蜂蜜在Luria肉汤(LB)中溶解对铜绿假单胞菌(Pseudomonas aeruginosa)培养物生物膜的影响。一种类型的培养物(ATCC 27853)和5种临床分离物分别来自5个不同的感染伤口患者,在微滴板中37℃在LB中孵育24小时,以使生物膜形成。然后将培养基替换为LB,或含有20% (w/v)麦卢卡蜂蜜的LB或含有40% (w/v)麦卢卡蜂蜜的LB,并在37℃下孵育24小时。用2.5% (w/v)戊二醛固定贴壁细胞,并用0.25% (w/v)结晶紫染色,每隔一段时间监测生物膜生物量。与LB中单独接触40% (w/v)麦卢卡蜂蜜和20% (w/v)麦卢卡蜂蜜相比,铜绿假单胞菌生物膜暴露于LB中40% (w/v)麦卢卡蜂蜜导致所有培养物的生物膜生物量显著减少(p >0.05)。暴露时间为9至11小时后,生物膜生物量的差异最为显著。这一初步研究表明麦卢卡蜂蜜具有控制慢性伤口生物膜的潜力,并证明了这一主题的进一步研究是合理的。
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引用次数: 32
Quality and standardisation of Royal Jelly 蜂王浆的质量与标准化
Pub Date : 2009-02-10 DOI: 10.3896/IBRA.4.01.1.04
A. Sabatini, G. Marcazzan, M. Caboni, S. Bogdanov, L. B. Almeida-Muradian
Departamento de Alimentos e Nutricao Experimental, Faculdade de Ciencias Farmaceuticas da Universidade de Sao Paulo (USP), Sao Paulo, Brazil. Received 26 June 2008, accepted subject to revision 18 August 2008, accepted for publication November 2008. *Corresponding author: Email: annagloria.sabatini@entecra.it
圣保罗大学(USP)医药科学学院,巴西圣保罗。2008年6月26日收稿,2008年8月18日修订后接受,2008年11月接受发表。*通讯作者:Email: annagloria.sabatini@entecra.it
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引用次数: 208
Prologue: Honey and healing through the ages 序曲:蜂蜜和疗愈贯穿千古
Pub Date : 2009-02-10 DOI: 10.3896/IBRA.4.01.1.02
Richard Jones
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引用次数: 68
期刊
Journal of Apiproduct and Apimedical Science
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