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A randomized control trial comparing the effects of manuka honey and tualang honey on wound granulation of post debridement diabetic foot wounds 一项比较麦卢卡蜂蜜和土浪蜂蜜对糖尿病足创面清创后创面肉芽肿影响的随机对照试验
Pub Date : 2011-01-01 DOI: 10.3896/IBRA.4.03.1.04
S. Nawfar, C. S. Han, Mohammad Paiman, Mohd skandar
The concern of a surgeon dealing with the management of diabetic ulcers is to get the wound debrided and dressed until it granulates. After this stage a proper tissue cover with skin graft is among the options available to encourage the wounds to heal. A resurgence of interest and an increasing number of case reports on the use of honey on diabetic foot ulcers, reflect a growing awareness and a need for cost-effective therapies. Given honey's great potential as an alternative in wound dressing, this double-blinded randomized controlled study was designed to investigate the wound healing property and the granulation tissue promoting effect of honey, comparing the local Malaysian tualang honey with the well-established manuka honey in the management of patients with diabetic foot wounds. Thirty-four patients with Wagner stage II or III diabetic foot ulcers were enrolled in the study, randomized into 2 groups of seventeen patients, treated with either manuka honey or tualang honey dressing on a daily basis post surgical debridement. Wound healing was assessed by measuring the granulation surface area utilizing a tracing technique. The primary outcome measure which was area of coverage with new granulation tissue was checked in each group after seven days. Other variables which might affect wound healing that were considered as confounders were recorded and analysed as well as the primary outcome. There was no significant difference (p=0.687) between manuka honey and tualang honey group in terms of mean percentage of granulation tissue surface area after one week of dressing in diabetic foot ulcers (manuka group 60.7%, tualang group 57.0%). All variables in both groups which were age, wound size, HbA1c, haemoglobin level, serum albumin level, absolute lymphocyte count and ankle-brachial systolic pressure index (ABSI), were comparable and were found not to be statistically significant to influence the primary outcome. Tualang honey induced granulation and exhibited beneficial action in promoting wound healing which was comparable to the more established manuka honey. The result suggests that tualang honey could be used as an alternative therapeutic agent for diabetic foot wounds with similar beneficial effects as those expected for manuka honey.
处理糖尿病溃疡的外科医生所关心的是对伤口进行清创和包扎,直到它形成颗粒。在这个阶段之后,用皮肤移植的适当组织覆盖是促进伤口愈合的可用选择之一。对使用蜂蜜治疗糖尿病足溃疡的兴趣的复苏和越来越多的病例报告,反映了人们日益提高的认识和对具有成本效益的疗法的需求。考虑到蜂蜜作为伤口敷料替代品的巨大潜力,本双盲随机对照研究旨在研究蜂蜜的伤口愈合特性和促进肉芽组织的作用,比较马来西亚当地的土alang蜂蜜和成熟的麦卢卡蜂蜜在糖尿病足伤口治疗中的作用。34名患有Wagner II期或III期糖尿病足溃疡的患者被纳入研究,随机分为两组,每组17名患者,在手术清创后每天使用麦卢卡蜂蜜或土朗蜂蜜敷料治疗。利用追踪技术测量肉芽表面面积来评估伤口愈合情况。主要结局指标是新肉芽组织覆盖面积,每组在7天后进行检查。记录和分析其他可能影响伤口愈合的混杂因素以及主要结局。麦卢卡蜂蜜组与土alang蜂蜜组在糖尿病足溃疡治疗1周后肉芽组织表面积平均百分比(麦卢卡组60.7%,土alang组57.0%)差异无统计学意义(p=0.687)。两组中年龄、伤口大小、HbA1c、血红蛋白水平、血清白蛋白水平、绝对淋巴细胞计数和踝-肱收缩压指数(ABSI)的所有变量均具有可比性,且对主要结局的影响无统计学意义。土朗蜂蜜诱导肉芽形成,并表现出促进伤口愈合的有益作用,这与更成熟的麦卢卡蜂蜜相当。结果表明,土朗蜂蜜可作为糖尿病足部伤口的替代治疗剂,其有益效果与麦卢卡蜂蜜相似。
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引用次数: 5
The Efficacy of Tualang Honey in Comparison to Silver in Dressing Wounds in Rats 土郎蜂蜜与银在大鼠创面敷料中的作用比较
Pub Date : 2011-01-01 DOI: 10.3896/IBRA.4.03.1.08
Mat Saad Arman Zaharil, Azman Ws, W. Sulaiman, A. Halim, Mohd Yussof Shah, J. Hasnan
Summary Tualang honey is obtained from large honeycombs produced by Asian bees (Apis dorsata) in gigantic Tualang trees. It has been used traditionally by local communities to treat wounds. However, unlike manuka honey its medicinal uses are not well researched. An open, prospective study into the efficacy of wound healing in full thickness wounds in rats, was designed to compare two honey impregnated dressings with silver-impregnated hydrofibre dressings. A full-thickness wound was created on the dorsum of Sprague-Dawley rats (n=45). Tualang honey impregnated paraffin tulle (P-honey) and tualang honey impregnated hydrofibre dressings (H-honey) were compared with silver-containing hydrofibre dressing (positive control; H-Ag). The wounds were inspected on days 4, 7, 14, 21 and 28. The dressings and wounds were assessed for adherence, ease of removal, fluid accumulation, dryness of skin and exudates, rate of epithelization, healing and wound contraction. Three rats treated with each dressing were sacrificed on the days that wounds were inspected. The wounds and scars were histologically analysed for inflammatory parameters. Tualang honey impregnated dressings were comparable to the commercially available silver impregnated hydrofibre dressing in terms of adherence, ease of removal, fluid accumulation, dryness of surrounding skin and exudates; p > 0.05 for non-parametric Kruskal-Wallis tests and post hoc corrections with a Mann-Whitney test. The rates of wound healing, wound contracture and subsequent histological analysis of inflammatory reaction by each dressing were also comparable. Tualang honey impregnated dressings were as effective as silver impregnated hydrofibre dressings in terms of dressing properties, promotion of wound healing and inflammatory reaction.
土郎蜂蜜是由亚洲蜜蜂(Apis dorsata)在巨大的土郎树上生产的大蜂巢中获得的。传统上,当地社区用它来治疗伤口。然而,与麦卢卡蜂蜜不同的是,它的药用用途并没有得到很好的研究。一项开放的前瞻性研究,旨在比较两种蜂蜜浸渍敷料和浸渍银纤维敷料对大鼠全层伤口愈合的功效。在Sprague-Dawley大鼠背部创面全层创面(n=45)。将土郎蜂蜜浸渍石蜡纱(P-honey)和土郎蜂蜜浸渍纤维敷料(H-honey)与含银纤维敷料(阳性对照;表面抗原)。分别于第4、7、14、21、28天检查伤口。评估敷料和伤口的粘附性、易去除性、液体积聚、皮肤和渗出物的干燥程度、上皮化率、愈合和伤口收缩。每种敷料处理3只大鼠,在检查伤口的当天处死。对伤口和疤痕的炎症参数进行组织学分析。Tualang蜂蜜浸渍敷料与市售的银浸渍纤维敷料在粘附性、易去除性、积液性、周围皮肤干燥性和渗出物方面相当;非参数Kruskal-Wallis检验和Mann-Whitney检验的事后校正p > 0.05。伤口愈合率、伤口挛缩率和随后各敷料炎症反应的组织学分析也具有可比性。土朗蜂蜜浸渍敷料在敷料性能、促进伤口愈合和炎症反应方面与浸渍银纤维敷料效果相当。
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引用次数: 10
Effect of Honey on Testicular Functions in Rats Exposed to Cigarette Smoke 蜂蜜对吸烟大鼠睾丸功能的影响
Pub Date : 2011-01-01 DOI: 10.3896/IBRA.4.03.1.03
Mohamed Mahaneem, S. A. Sulaiman, H. Jaafar, Kuttulebbai Nainamohamed, Salam Sirajudeen, Z. Ismail, M. N. Islam
Summary Honey is traditionally consumed by the local Malaysian population as a nutrient, as well as for the enhancement of fertility. The decline in male reproductive health and fertility in the last 30 years has been linked to environmental toxicants including cigarette smoke (CS). In human and experimental studies, CS exposure has been associated with decreased plasma testosterone level, lower sperm count and increased percentage of abnormal sperm. The aim of this study, therefore, was to determine the possible protective role of honey against the toxic effects of CS on testicular functions in rats. Thirty-two adult Sprague-Dawley rats were randomly divided into 4 groups (8 rats per group) i.e. control group, honey-treated group (H), cigarette smoke-exposed group (CS) and honey-treated plus CS-exposed group (H+CS). Rats in control and CS groups received oral administration of distilled water daily while rats in H and H+CS groups received honey (1.2 g/kg body weight) orally by gavage daily. Rats in CS and H+CS groups were also exposed to CS for 8 min (3 times/day). After 13 weeks of treatment, each rat was sacrificed for reproductive parameters analysis. Rats in CS group had significantly lower sperm count, daily sperm production, percentage of motile sperm and testosterone level as well as a higher percentage of abnormal sperm compared to control and H groups. However, supplementation of honey significantly improved all these parameters in H+CS group. Administration of honey significantly attenuated the toxic effects of CS on spermatogenesis and testosterone level in rats. This study suggests that honey might have a protective effect against CS-induced impaired testicular functions in rats.
传统上,马来西亚当地居民将蜂蜜作为一种营养物质,以及提高生育能力。过去30年来,男性生殖健康和生育率的下降与包括香烟烟雾在内的环境毒物有关。在人体和实验研究中,CS暴露与血浆睾酮水平降低、精子数量减少和异常精子百分比增加有关。因此,本研究的目的是确定蜂蜜对CS对大鼠睾丸功能的毒性作用的可能保护作用。将32只成年sd大鼠随机分为4组,即对照组、蜂蜜处理组(H)、香烟烟雾暴露组(CS)和蜂蜜+CS暴露组(H+CS),每组8只。对照组和CS组大鼠每天口服蒸馏水,H组和H+CS组大鼠每天灌胃蜂蜜(1.2 g/kg体重)。CS组和H+CS组大鼠同样暴露于CS 8 min(3次/天)。治疗13周后处死各组大鼠进行生殖参数分析。与对照组和H组相比,CS组大鼠的精子数量、每日精子产量、活动精子百分比和睾丸激素水平显著降低,异常精子百分比较高。然而,在H+CS组中,添加蜂蜜显著改善了所有这些参数。蜂蜜可显著降低CS对大鼠精子发生和睾酮水平的毒性作用。本研究提示蜂蜜可能对cs诱导的大鼠睾丸功能受损有保护作用。
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引用次数: 46
The Benefit of Tualang Honey in Reducing Acute Respiratory Symptons Among Malaysian Hajj Pilgrims: A Preliminary Study 土朗蜂蜜对减轻马来西亚朝觐朝圣者急性呼吸道症状的益处:初步研究
Pub Date : 2011-01-01 DOI: 10.3896/IBRA.4.03.1.07
S. A. Sulaiman, H. Hasan, Z. Deris, M. S. Wahad, Ruhana Che Yusof, N. Naing, N. Othman
Siti Amrah Sulaiman, Habsah Hasan, Zakuan Zainy Deris, Mohd Suhaimi Abdul Wahab, Ruhana Che Yusof, Nyi Nyi Naing and Nor Hayati Othman 1 Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia 2 Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia 3 Unit of Biostatistics and Research Methodology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia 4 Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia
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引用次数: 17
Honey as a treatment option for rhinoconjunctivitis 蜂蜜作为鼻结膜炎的一种治疗选择
Pub Date : 2010-10-01 DOI: 10.3896/IBRA.4.02.4.02
K. Münstedt, M. Kalder
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引用次数: 3
The antifungal activity of Iranian propolis samples against fluconazole-resistant Candida albicans strains isolated from HIV+ patients with oropharyngeal candidiasis 伊朗蜂胶样品对HIV阳性口咽念珠菌病耐氟康唑白色念珠菌的抑菌活性
Pub Date : 2010-10-01 DOI: 10.3896/IBRA.4.02.4.05
Roozbeh Yarfani, A. Khosravi, H. Shokri
Propolis is an antimicrobial agent whose composition can vary depending on the area from which it is collected. The aim of this study was to determine the in vitro activity of two propolis samples from northern and southern Iran (Mazandaran and Hormozgan province respectively) against some fluconazole-resistant Candida albicans strains isolated from HIV patients with oropharyngeal candidiasis (OPC). The chemical composition of propolis samples was determined by high-resolution gas chromatography coupled to mass spectrometry (GC–MS). Ethanolic extracts were prepared from the propolis samples. The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of propolis on the growth of C. albicans isolates were determined using the broth microdilution method. The main compounds of propolis extracts were aromatic acids such as caffeic acid (1.6% for northern and 2.2% for southern samples). The propolis extracts showed antifungal activities; the concentrations capable of inhibiting all of the yeasts ranged from 2 to 20 mg/mL. Propolis from southern Iran showed the most effective MIC values for the yeasts studied. Because of increased antifungal resistance, propolis may be kept in mind in the treatment of oral cavity diseases such as OPC in HIV patients.
蜂胶是一种抗菌剂,其成分可以根据其收集的区域而变化。本研究的目的是测定来自伊朗北部和南部(分别为Mazandaran省和Hormozgan省)的两种蜂胶样品对从HIV口咽念珠菌病(OPC)患者中分离的一些氟康唑耐药白色念珠菌菌株的体外活性。采用高分辨率气相色谱-质谱联用技术(GC-MS)测定蜂胶样品的化学成分。从蜂胶样品中提取乙醇提取物。采用肉汤微量稀释法测定蜂胶对白色念珠菌分离株生长的最低抑菌浓度(mic)和最低杀真菌浓度(mfc)。蜂胶提取物的主要成分为芳香酸,如咖啡酸(北方样品占1.6%,南方样品占2.2%)。蜂胶提取物具有抗真菌活性;能够抑制所有酵母的浓度范围为2 ~ 20mg /mL。来自伊朗南部的蜂胶对所研究的酵母显示出最有效的MIC值。由于抗真菌耐药性增加,蜂胶在治疗艾滋病毒患者的OPC等口腔疾病时可能会被记住。
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引用次数: 11
What is the future of Bee-Pollen? 蜂花粉的未来是什么?
Pub Date : 2010-10-01 DOI: 10.3896/IBRA.4.02.4.01
M. Campos, Christian Frigerio, J. Lopes, S. Bogdanov
Maria Graça R. Campos, Christian Frigerio, Joana Lopes and Stefan Bogdanov 1 Centre of Pharmaceutical Studies, Laboratory of Pharmacognosy and Phytotherapy, Faculty of Pharmacy – R. do Norte University of Coimbra, 3000-295 Coimbra, Portugal 2 REQUIMTE, Departamento de Química-Física, Faculdade de Farmacía, Universidade do Porto, Rua Aníbal Cunha 164, Porto 4050-047, Portugal 3 Bee Products Science, 3127 Mühlethurnen, Switzerland
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引用次数: 138
Inbred mice fed only bee pollen 近亲繁殖的老鼠只吃蜂花粉
Pub Date : 2010-10-01 DOI: 10.3896/IBRA.4.02.4.04
R. Liebelt
We previously reported that three different inbred strains of mice (CBA/Ki, C3H/f/Ki and C57BI/Ki) survived in a healthy condition when fed only a bee pollen granules diet and drinking water for 365 days. Similarly, Sprague-Dawley rats showed comparable skeletal and organ growth and development when fed a similar bee pollen diet during a twelve-week period as compared to control animals fed a standard laboratory diet. It was the purpose of this study to determine how long the survival time of CBA/Ki mice could be extended beyond 365 days when fed only bee pollen granules and water as compared to controls. Control mice survived a mean of 477 days (389-548) with 100% diagnosed with renal amyloidosis at autopsy which characterized this strain of mice in our laboratory. All pollen fed mice appeared healthy when euthanized at 600 days of age. Survival times were compared with a log rank test (p < 0.001). Also, there was no evidence of pathology particularly in the kidneys. These unexpected findings could be interpreted as being consistent with the genetotrophic disease concept proposed more than fifty years ago, namely, that bee pollen contains either a unique nutrient or a higher level of one or more nutrients that may be lacking or at a lower concentration in the standard diet which will then circumvent partial genetic blocks in the metabolic assembly line. If correct, this finding could provide an experimental model for study in the emerging field of nutrigenomics.
我们之前报道了三种不同的近交系小鼠(CBA/Ki, C3H/f/Ki和C57BI/Ki)在仅喂食蜂花粉颗粒和饮用水的情况下健康存活365天。同样,在为期12周的时间里,与喂食标准实验室食物的对照动物相比,喂食类似蜂花粉的Sprague-Dawley大鼠的骨骼和器官生长发育情况相似。本研究的目的是确定仅饲喂蜂花粉颗粒和水与对照组相比,CBA/Ki小鼠的存活时间可以延长多久,超过365天。对照小鼠平均存活477天(389-548),尸检时100%诊断为肾淀粉样变,这是我们实验室该品系小鼠的特征。所有喂食花粉的小鼠在600日龄时被安乐死时表现健康。生存时间比较采用对数秩检验(p < 0.001)。此外,没有病理证据,特别是在肾脏方面。这些意想不到的发现可以解释为与五十多年前提出的遗传营养疾病概念相一致,即蜂花粉含有一种独特的营养物质或一种或多种营养物质的较高水平,这些营养物质在标准饮食中可能缺乏或浓度较低,从而绕过代谢装配线中的部分遗传块。如果正确的话,这一发现可以为新兴的营养基因组学领域的研究提供一个实验模型。
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引用次数: 0
Effect of Jungle honey on the chemotactic activity of neutrophils 丛林蜂蜜对中性粒细胞趋化活性的影响
Pub Date : 2010-10-01 DOI: 10.3896/IBRA.4.02.4.03
Mayuko Miyagawa, M. Fukuda, Y. Hirono, Ayaka Kawazoe, E. Shigeyoshi, M. Sakura, T. Takeuchi, O. Mazda, K. Pinkerton, M. Takeuchi
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引用次数: 0
Experimental use of oral propolis for prevention of radiation oesophagitis in patients treated with chest radiotherapy 口服蜂胶预防胸部放疗患者放射性食道炎的实验研究
Pub Date : 2010-07-01 DOI: 10.3896/IBRA.4.02.3.03
F. Meroni, R. Alloni, M. Ciresa, A. Scrocca, L. Trodella
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引用次数: 1
期刊
Journal of Apiproduct and Apimedical Science
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