Pub Date : 2014-01-01DOI: 10.3923/JAVAA.2014.859.863
M. Valdovinos, C. L. Flores, Jorge Galindo García, D. S. Chiprés, Theodor Duifhuis Rivera, R. A. Estrella, J. Pineda, Javier Germán Rodríguez Carpena
The purpose of this study was to assess the frequency of Theileria equi in horses imported into Mexico. During different stages of quarantine, 348 blood samples were taken from clinically healthy horses imported into Mexico from 2011 to 2013. Nested PCR (nPCR) of the Merozoite Antigen-1 (EMA-1) gene was performed for pathogen detection. In total, 93 horses tested positive for T. equi resulting in a 26.72% frequency with a 95% Confidence Interval (CI) of 22.07-31.37%. This is the first molecular diagnostics study to identify T. equi-positive horses imported into Mexico these results highlight the importance of nPCR analysis for T. equi in clinically healthy imported horses.
{"title":"NESTED PCR DETECTION OF THEILERIA EQUI INFECTION AND FREQUENCY IN HORSES IMPORTED INTO MEXICO","authors":"M. Valdovinos, C. L. Flores, Jorge Galindo García, D. S. Chiprés, Theodor Duifhuis Rivera, R. A. Estrella, J. Pineda, Javier Germán Rodríguez Carpena","doi":"10.3923/JAVAA.2014.859.863","DOIUrl":"https://doi.org/10.3923/JAVAA.2014.859.863","url":null,"abstract":"The purpose of this study was to assess the frequency of Theileria equi in horses imported into Mexico. During different stages of quarantine, 348 blood samples were taken from clinically healthy horses imported into Mexico from 2011 to 2013. Nested PCR (nPCR) of the Merozoite Antigen-1 (EMA-1) gene was performed for pathogen detection. In total, 93 horses tested positive for T. equi resulting in a 26.72% frequency with a 95% Confidence Interval (CI) of 22.07-31.37%. This is the first molecular diagnostics study to identify T. equi-positive horses imported into Mexico these results highlight the importance of nPCR analysis for T. equi in clinically healthy imported horses.","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.3923/JAVAA.2014.891.896
M. Y. Al-Saiady, H. H. Mogawer, S. Al-Mutairi, M. Bengoumi, B. Faye, A. Musaad, A. Gar-Elnaby
The aim of the present study was to investigate the effect of nutrition on body weight gain, ovarian development, blood components (total protein, albumen, globulin, cholesterol, glucose) and hormone (estradiol, progesterone, FSH, LH, GnRH and Leptin) levels in peri-pubertal female camels. Fourteen dromedary females (Camelus dromedarius) were divided into two groups (A and B) with respective average body weight and age of 381 kg, 24 months and 458 kg, 24 months at the start of the experiment. Group A received a diet with 13% Crude Protein (CP) and 2.9 Mcal Metabolizable Energy (ME). Group B received the traditional diet of the farm with 12.43 CP and 2.7 ME. Both diets contained 1:3 forage:concentrate ratio. Individual feed intake was calculated after a 14 days adaptation period. Feed offered and orts were recorded daily during the entire experimental period of 24 months. Blood samples were taken from the same 5 animals in each group at 15 day intervals throughout the experimental period. Hormone concentrations were measured using specific ELISA kits. Initial body weight, final body weight and average daily gain for the two treatment groups were not significantly different. Group B had greater ovarian size than group A but the difference was also non-significant. The size of the right ovary was less than the left ovary. Season had no significant effect on ovary's size. Group A camels tended to have higher blood estradiol, leptin, GnRh and LH levels than group B. However, the differences were not significant except in the case of estradiol. Progesterone and FSH levels were also comparable in the two groups. It was concluded that the feeding regimen used in this study did not significantly affect body weight, daily weight gain and blood progesterone level while a relative, though statistically non-significant, increase was recorded in blood estrogen, Leptin, GnRH and LH levels in group A. (Resume d'auteur)
{"title":"Dietary and seasonal effects on body weight, ovarian development and blood reproductive hormone levels in peri-pubertal female camels (Camelus dromedarius)","authors":"M. Y. Al-Saiady, H. H. Mogawer, S. Al-Mutairi, M. Bengoumi, B. Faye, A. Musaad, A. Gar-Elnaby","doi":"10.3923/JAVAA.2014.891.896","DOIUrl":"https://doi.org/10.3923/JAVAA.2014.891.896","url":null,"abstract":"The aim of the present study was to investigate the effect of nutrition on body weight gain, ovarian development, blood components (total protein, albumen, globulin, cholesterol, glucose) and hormone (estradiol, progesterone, FSH, LH, GnRH and Leptin) levels in peri-pubertal female camels. Fourteen dromedary females (Camelus dromedarius) were divided into two groups (A and B) with respective average body weight and age of 381 kg, 24 months and 458 kg, 24 months at the start of the experiment. Group A received a diet with 13% Crude Protein (CP) and 2.9 Mcal Metabolizable Energy (ME). Group B received the traditional diet of the farm with 12.43 CP and 2.7 ME. Both diets contained 1:3 forage:concentrate ratio. Individual feed intake was calculated after a 14 days adaptation period. Feed offered and orts were recorded daily during the entire experimental period of 24 months. Blood samples were taken from the same 5 animals in each group at 15 day intervals throughout the experimental period. Hormone concentrations were measured using specific ELISA kits. Initial body weight, final body weight and average daily gain for the two treatment groups were not significantly different. Group B had greater ovarian size than group A but the difference was also non-significant. The size of the right ovary was less than the left ovary. Season had no significant effect on ovary's size. Group A camels tended to have higher blood estradiol, leptin, GnRh and LH levels than group B. However, the differences were not significant except in the case of estradiol. Progesterone and FSH levels were also comparable in the two groups. It was concluded that the feeding regimen used in this study did not significantly affect body weight, daily weight gain and blood progesterone level while a relative, though statistically non-significant, increase was recorded in blood estrogen, Leptin, GnRH and LH levels in group A. (Resume d'auteur)","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-11-19DOI: 10.3923/JAVAA.2013.712.717
K. Sawada, K. Nagano, N. Nishino
{"title":"Plasma 3-Methylhistidine concentration in peripartum dairy cows given diets with two protein levels.","authors":"K. Sawada, K. Nagano, N. Nishino","doi":"10.3923/JAVAA.2013.712.717","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.712.717","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-07-31DOI: 10.3923/JAVAA.2013.34.37
Hyunsoo Kim, Youn Wha Kim
{"title":"Hypergravity induces overexpression of inducible nitric oxide synthase in mouse livers","authors":"Hyunsoo Kim, Youn Wha Kim","doi":"10.3923/JAVAA.2013.34.37","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.34.37","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3923/JAVAA.2013.1260.1262
A. Janiszewski, U. Pasławska, R. Pasławski, A. Cepiel, A. Noszczyk-Nowak, L. Kiczak, J. Nicpoń, W. Witkiewicz
{"title":"Normal M-mode echocardiography of mitral valve in adult domestic swine","authors":"A. Janiszewski, U. Pasławska, R. Pasławski, A. Cepiel, A. Noszczyk-Nowak, L. Kiczak, J. Nicpoń, W. Witkiewicz","doi":"10.3923/JAVAA.2013.1260.1262","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.1260.1262","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3923/JAVAA.2013.1550.1564
M. Renna, C. Lussiana, M. D'Agostino, A. Mimosi, R. Fortina
{"title":"Extruded Linseed Supplementation in Dairy Goat Diet: Effects on Productive Performance and Fatty Acid Profile of Bulk Milk, Fresh and Ripened Cheese","authors":"M. Renna, C. Lussiana, M. D'Agostino, A. Mimosi, R. Fortina","doi":"10.3923/JAVAA.2013.1550.1564","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.1550.1564","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70126985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3923/JAVAA.2013.539.543
Rongli You, L. Lv, Zhixue Cheng, Jun-ping He, George W. Smith, C. Dong
{"title":"Application of ultrasonography for early pregnancy diagnosis in alpacas (lamapacos)","authors":"Rongli You, L. Lv, Zhixue Cheng, Jun-ping He, George W. Smith, C. Dong","doi":"10.3923/JAVAA.2013.539.543","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.539.543","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3923/JAVAA.2013.269.275
Duran Özkök, K. Işcan, S. Silici
{"title":"Effects of Dietary Propolis Supplementation on Performance and Egg Quality in Laying Hens","authors":"Duran Özkök, K. Işcan, S. Silici","doi":"10.3923/JAVAA.2013.269.275","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.269.275","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3923/JAVAA.2013.181.185
Ilenia Copponi, S. Preziuso, M. Cerquetella, F. Laus, M. Bordi, A. Spaterna, Cuteri
{"title":"Evaluation of Lysine and Lysine-Lactoferrin Association in Cats Infected by Feline Herpesvirus-1","authors":"Ilenia Copponi, S. Preziuso, M. Cerquetella, F. Laus, M. Bordi, A. Spaterna, Cuteri","doi":"10.3923/JAVAA.2013.181.185","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.181.185","url":null,"abstract":"","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70126995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3923/JAVAA.2013.683.688
E. D. Toit, J. Oguttu
The calpain and calpastatin proteolytic enzyme system is believed to be the main contributor to the tenderness of meat at post mortem. However, little is known about the enzyme calpain and its inhibitor calpastatin and how the two influence meat tenderness. This could be due to the fact that the study of the technology to understand calpain system is still relatively new. When factors that influence conversion of muscle to meat, meat tenderization and the activity of calpain and calpastatin are considered, it is evident that that there is overlap of the factors involved in the three aspects. However, not all factors that influence meat tenderization have been shown to affect the calpain and calpastatin enzymatic system. Thought there are studies that demonstrate how diet, growth promoters, gender, weather, handling of animals and electrical stimulation influence the calpain and calpastastin enzymatic system, these studies are not conclusive. In view of this, further studies are need particularly in South Africa to understand the system fully under local conditions and in local beef cattle breeds such as the Nguni, Africander and Bosmara. Furthermore, there is a need for work to establish the relationship between pH and the activity of calpain and calpastatins give the conflicting views held on this aspect.
{"title":"Calpain and calpastatin activity post mortem and meat tenderness","authors":"E. D. Toit, J. Oguttu","doi":"10.3923/JAVAA.2013.683.688","DOIUrl":"https://doi.org/10.3923/JAVAA.2013.683.688","url":null,"abstract":"The calpain and calpastatin proteolytic enzyme system is believed to be the main contributor to the tenderness of meat at post mortem. However, little is known about the enzyme calpain and its inhibitor calpastatin and how the two influence meat tenderness. This could be due to the fact that the study of the technology to understand calpain system is still relatively new. When factors that influence conversion of muscle to meat, meat tenderization and the activity of calpain and calpastatin are considered, it is evident that that there is overlap of the factors involved in the three aspects. However, not all factors that influence meat tenderization have been shown to affect the calpain and calpastatin enzymatic system. Thought there are studies that demonstrate how diet, growth promoters, gender, weather, handling of animals and electrical stimulation influence the calpain and calpastastin enzymatic system, these studies are not conclusive. In view of this, further studies are need particularly in South Africa to understand the system fully under local conditions and in local beef cattle breeds such as the Nguni, Africander and Bosmara. Furthermore, there is a need for work to establish the relationship between pH and the activity of calpain and calpastatins give the conflicting views held on this aspect.","PeriodicalId":14914,"journal":{"name":"Journal of Animal and Veterinary Advances","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70127345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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