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ASSOCIATION OF CHANGE IN BODY CONDITION SCORE WITH MILK YIELD AND REPRODUCTION TRAITS OF HOLSTEIN COWS 荷斯坦奶牛体况评分变化与产奶量及繁殖性状的关系
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.2.0045
E. Kul, A. Şahin, Emre Uğurlutepe, M. Soydaner
The aim of the study was to determine the effect of changes in body condition score (BSC) on milk yield and reproduction traits in early (30, 60 and 90 d), mid (120 and 150 d) and late lactation (210 and 270 d) in Holstein cows raised on a private dairy cattle farm in Kırşehir, Turkey. The data were obtained from 140 cows calved from November 2014 to November 2015. BSC groups were categorized as low, moderate and high in early lactation (<2.75, 2.75–3.00 and >3.00), midlactation (<3.00, 3.00–3.25 and >3.25) and late lactation (<3.25, 3.25–3.50 and >3.50). Cows with low and moderate BCS in early (BCS<2.75 and BCS=2.75-3.00), mid (BCS<3.00 and BCS=3.00–3.25) and late lactation (BCS<3.25 and BCS>3.50) had a higher daily milk yield (DMY), lactation milk yield (LMY) and 305-day milk yield (305-dMY) (P<0.05). Reproduction traits were also affected (P<0.05) by BCS in mid-lactation. In mid-lactation, the cows with low and moderate BCS had a shorter period of calving to the first service interval (CFSI) and lower number of services per conception (NSC) than that of the cows with high BCS (P<0.05). Both milk yield and reproduction traits were adversely affected by high BCS. It is suggested that BCS can be used as a reliable indicator in indirect selection programs for higher milk yield and reproductive performance in Holstein cows.
本研究旨在确定体况评分(BSC)变化对土耳其Kırşehir一家私人奶牛场饲养的荷斯坦奶牛泌乳早期(30、60和90 d)、中期(120和150 d)和后期(210和270 d)产奶量和繁殖性状的影响。数据来自2014年11月至2015年11月出生的140头奶牛。泌乳早期(3.00)、泌乳中期(3.25)和泌乳后期(3.50)BSC组分为低、中、高3组。早期低BCS和中等BCS (BCS3.50)奶牛的日产奶量(DMY)、泌乳量(LMY)和305日产奶量(305-dMY)较高(P<0.05)。泌乳中期BCS对繁殖性状也有影响(P<0.05)。泌乳中期,低BCS和中等BCS奶牛的产犊周期至第一胎龄(CFSI)短于高BCS奶牛(P<0.05),单胎胎龄数(NSC)低。高BCS对产奶量和繁殖性状均有不利影响。因此,BCS可作为荷斯坦奶牛提高产奶量和繁殖性能的一个可靠的间接选择指标。
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引用次数: 0
EFFECT OF ASCORBIC ACID AND/OR ALPHA-TOCOPHEROL FORTIFICATION ON SEMEN QUALITY, METABOLIC PROFILE, ANTIOXIDANTS STATUS, AND DNA OF ROOSTERS EXPOSED TO HEAT STRESS 强化抗坏血酸和/或α -生育酚对热应激公鸡精液质量、代谢特征、抗氧化剂状态和DNA的影响
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.2.0051
Y. Attia, B. Abou-shehema, A. A. Abdellah, O. Aly, Asmaa Sh. El-Naggar
This study investigates the influence of heat stress (HS) and vitamin C (VC) and/or vitamin E (VE) on semen quality, biochemical constituents of seminal and blood plasma, antioxidant status and jejunum and ilium deoxyribonucleic acid (DNA) of roosters. Forty-five, 32-week-old roosters were divided into five groups of nine replicates. One group was kept under a thermoneutral environment, whereas the other four were kept under HS. One of the four groups served as an unsupplemented HS group and was fed the basal diet only. The other three HS groups were supplemented with VC (200 mg/kg ascorbic acid and/or VE 150 mg/kg α-tocopherol acetate). Vitamins fortification completely restored sperm liveability , semen pH, fertility, seminal plasma total protein and globulin to the control level. Vitamins fortification completely recovered seminal plasma and blood plasma total antioxidant capacity (TAC) and malondialdehyde (MDA), red blood cell (RBCs), haemoglobin (Hgb), packed cell volume (PCV), white blood cells (WBCs), blood plasma glucose, lipid profiles, liver enzymes, and renal function. Heterophil/lymphocyte (H/L) ratio was totally restored following VE fortification. Vitamins fortification significantly increased jejunum and ileum DNA compared with the HS group, with VC inducing a complete recovery. In conclusion, either 200 mg /kg VC or 150 mg/kg VE improved semen quality, fertility, seminal plasma and blood biochemistry and haematology of HS roosters, with the VE group yielding
本试验研究了热应激(HS)和维生素C (VC)和/或维生素E (VE)对公鸡精液质量、精液和血浆生化成分、抗氧化状态以及空肠和回肠脱氧核糖核酸(DNA)的影响。选用45只32周龄公鸡,随机分为5组,每组9个重复。一组保持在热中性环境下,另外四组保持在HS环境下。其中1组为不添加HS组,仅饲喂基础饲粮。其余3个HS组分别添加VC (200 mg/kg抗坏血酸和/或VE 150 mg/kg α-生育酚醋酸酯)。强化维生素可使精子存活率、精液pH值、生育能力、精浆总蛋白和球蛋白恢复到控制水平。维生素强化完全恢复了精浆和血浆总抗氧化能力(TAC)和丙二醛(MDA)、红细胞(rbc)、血红蛋白(Hgb)、堆积细胞体积(PCV)、白细胞(wbc)、血浆葡萄糖、血脂、肝酶和肾功能。强化VE后,嗜淋巴细胞/淋巴细胞(H/L)比值完全恢复。与HS组相比,维生素强化显著增加了空肠和回肠DNA, VC诱导了完全恢复。综上所述,200 mg/kg VC或150 mg/kg VE均可提高HS公鸡的精液质量、生殖力、精浆和血液生化及血液学指标,且VE组产量较高
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引用次数: 6
Urinary reproductive hormones influence seed germination within diluted urine of heifers: alternative pregnancy diagnostic method 尿生殖激素影响小母牛稀释尿液中的种子萌发:替代妊娠诊断方法
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.35759/janmplsci.v46-1.3
I. Láznic̆ková, T. Fedorova, M. Štolcová, A. Kubátová
Experimental non-invasive pregnancy diagnostic techniques, such as seed germination testing in diluted urine, may serve as an alternative to standardized pregnancy diagnostic methods, but scientific validation of these methods is required. This study aimed to use Czech Fleckvieh heifers to investigate the influence of urinary reproductive hormones (oestrone sulphate, 17β-oestradiol, and pregnanediol3-glucuronide) on the germination success of seeds placed within their diluted urine, and further to verify the reliability of seed germination test for pregnancy diagnosis in this species. Mung bean and wheat seeds were germinated in two urine-water dilutions (1:4 and 1:14) for three days, using urine samples obtained from either pregnant or non-pregnant heifers. Germinated seeds were counted daily, and the shoot lengths were measured three days after placement in the urine. Levels of urinary reproductive hormones were determined using competitive heterogeneous enzyme immunoassays (EIAs). Despite the dilution rate used, final results indicated that the urine from pregnant heifers inhibited germination and growth in mung beans only. On the contrary, wheat germination rate was higher within the urine from pregnant females on days 1 and 2, when using a 1:14 dilution rate. For both seed species, correlations between urinary hormone profiles and seed germination parameters were found, depending on the day of the experiment and the rate of dilution used. The shoot lengths of mung bean seeds were significantly (P < 0.05) and negatively correlated with the profiles of all hormones analysed from the urine samples. Thus, urinary reproductive hormones influence seed germination and growth rate of wheat and mung beans, indicating the potential for this technique to be used for pregnancy determination in heifers.
实验性非侵入性妊娠诊断技术,如稀释尿液中的种子发芽测试,可以作为标准化妊娠诊断方法的替代方法,但这些方法需要科学验证。本研究以捷克Fleckvieh小母牛为研究对象,研究了尿液生殖激素(硫酸雌酮、17β-雌二醇和妊娠二醇-葡糖苷)对其稀释尿液中种子萌发成功的影响,并进一步验证种子萌发试验对该品种妊娠诊断的可靠性。绿豆和小麦种子在两种尿水稀释(1:4和1:14)中发芽3天,使用怀孕或未怀孕母牛的尿液样本。每天计数发芽的种子,并在放置在尿液中三天后测量芽长。采用竞争异质酶免疫分析法(EIAs)测定尿生殖激素水平。尽管使用了稀释率,但最终结果表明,怀孕母牛的尿液仅抑制绿豆的萌发和生长。相反,当使用1:14的稀释率时,第1天和第2天妊娠雌性尿液中的小麦发芽率较高。对于两种种子,尿激素谱和种子发芽参数之间的相关性被发现,这取决于实验的日期和使用的稀释率。绿豆种子的茎长与尿液中各激素含量呈显著负相关(P < 0.05)。因此,泌尿生殖激素会影响小麦和绿豆的种子萌发和生长速度,这表明该技术有可能用于小母牛的妊娠测定。
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引用次数: 1
EFFECT OF TRACE ELEMENT SUPPLEMENTATION ON THE GASTROINTESTINAL PARASITES OF GRAZING SHEEP 补充微量元素对放牧绵羊胃肠道寄生虫的影响
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.1.0009
Sajjad Ahmad, M. Sajid, M. K. Khan, R. Nadeem, H. Rizwan, M. Malik, F. Yosuf
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引用次数: 7
INFLUENCE OF DIETARY BETAINE ON NUTRIENT UTILIZATION AND DIGESTIVE ENZYME ACTIVITY IN YELLOW-FEATHER BROILERS DURING STARTER PHASE 饲粮中添加甜菜碱对黄羽肉鸡发酵期营养物质利用和消化酶活性的影响
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.1.0029
W. C. Liu, B. Ou, Y. Yuan, C. Sun, M. Xiao, Z. Zhao, L. An
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引用次数: 0
HABITAT SUITABILITY INDEX (HSI) MODEL OF PUNJAB URIAL (OVIS VEGNEI PUNJABIENSIS) IN PAKISTAN 巴基斯坦旁遮普野鸡生境适宜性指数(hsi)模型
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.1.0026
S. Suleman, W. Khan, K. M. Anjum, W. Shehzad, S. Hashmi
Unprecedented losses in biodiversity due to habitat loss and fragmentations in the current era have not only alarmed the scientists worldwide but also urged the need to devise suitable conservation strategies which are impossible without understanding the habitat requirements of the concerned species. Among many conservational methods Habitat suitability models are effective for identification of potential habitat and its relationship with the species. Punjab Urial is an endangered mammalian species of Kala Chitta Range and Salt Range in the Punjab, Pakistan. Maxent model was applied to identify the potential habitat by applying 120 sighting point, topographical and current bioclimatic variables. Possible area under receiver operating characteristics curves (AUC) test for current prediction is 0.988 that shows significant correlation between testing points and variables. In Pakistan total 3477 km area is suitable for Punjab Urial and potential habitats lie in limited areas in Rawalpindi, Sargodha, Gujrat, Mardan, Swabi, Nowshera, North Wziristan, Bannu, Dera Ismail Khan, Laki-Marwat and some area in Azad Jammu & Kashmir. Habitat of Punjab Urial is shrinking due to certain reasons including habitat fragmentation. In-situ and Ex-situ techniques are the major recommendation for species conservation in suitable and potential habitat of Punjab Urial.
在当今时代,由于栖息地的丧失和破碎造成了生物多样性的空前损失,这不仅使全世界的科学家感到震惊,而且敦促人们必须制定适当的保护战略,而如果不了解有关物种的栖息地要求,这些战略是不可能实现的。在众多的保护方法中,生境适宜性模型是识别潜在生境及其与物种关系的有效方法。旁遮普乌拉尔是巴基斯坦旁遮普Kala Chitta山脉和盐山脉的一种濒危哺乳动物。利用120个观察点、地形和当前生物气候变量,应用Maxent模型识别潜在栖息地。当前预测的受试者工作特征曲线(AUC)检验下的可能面积为0.988,测试点与变量之间存在显著的相关性。在巴基斯坦,总共3477公里的面积适合旁遮普的乌里尔河,潜在的栖息地位于拉瓦尔品第、萨戈达、古吉拉特邦、马尔丹、斯瓦比、瑙谢拉、北乌济里斯坦、班努、德拉伊斯梅尔汗、拉基马尔瓦特和阿扎德查谟和克什米尔的一些地区。由于栖息地破碎化等原因,旁遮普乌拉尔的栖息地正在萎缩。在旁遮普乌里尔河适宜和潜在生境中,就地和移地技术是物种保护的主要建议。
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引用次数: 2
CORRELATION OF MICROMETRIC PARAMETERS WITH QUALITY OF OOCYTES IN NILI-RAVI BUFFALO 水牛卵母细胞显微参数与卵母细胞质量的关系
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.1.0028
M. Nawaz, M. Saleem, M. Iqbal, A. Riaz
Detailed visibility of ooplasm plays a pivotal role in advanced reproductive bio techniques (ICSI, SCNT). To improve visibility, oocytes are usually handled after cumulus denuding. This study was conducted to establish buffalo oocyte quality estimation in denuded form. For this purpose, correlations were recognized among the size of oocyte, ooplasm and zona pellucida (ZP) with oocyte quality in Nili-Ravi buffalo. From slaughter house based ovaries, oocytes (n=150) were collected by aspiration of follicles and categorized into A, B, C, and D grades based on the number of layers of cumulus cell. After denuding with hyaluronidase enzyme, the detailed micrometric study was performed under inverted microscope at 40X by using micro imaging software (cellSens Dimension 1.16). The data were analyzed by correlation test using a statistical analysis system (SAS 9.1). The results demonstrated as the followings, the mean oocytes diameter (149.76±0.99μm), the width of zona pellucida (12.84±0.23μm), ooplasm diameter (117.5±0.70μm), oocyte area (17804±136μm2) and ooplasm area (11308.09±75μm). There was a strong positive correlation between the ooplasm diameter and oocytes diameter in A and B grade oocytes (r=0.7792) as compared to C and D grade oocytes (r=0.5967). However, there was moderate positive correlation between the width of zona pellucida and oocytes diameter in C and D grade oocytes (r=0.6530) as compared to the A and B grade oocytes (r=0.5287). In conclusion, the greater the ooplasm diameter and lesser the width of zona pellucida give rise to the good quality oocytes. Therefore, it is implied that for better developmental competence, the oocytes with bigger ooplasm and thinner zona pellucida should be processed.
卵浆的详细可见性在先进的生殖生物技术(ICSI, SCNT)中起着关键作用。为了提高能见度,卵母细胞通常在积云剥落后处理。本研究建立了水牛脱毛卵母细胞质量评价方法。为此,研究了尼利-拉维水牛卵母细胞大小、卵浆大小和透明带大小与卵母细胞质量的相关性。从屠宰场的卵巢中收集卵母细胞(n=150),通过卵泡抽吸收集,并根据积云细胞的层数分为A、B、C和D级。用透明质酸酶去角质后,在倒置显微镜下40倍放大镜下使用显微成像软件(cellSens Dimension 1.16)进行详细的显微研究。采用统计学分析系统(SAS 9.1)进行相关检验。结果表明:平均卵母细胞直径(149.76±0.99μm),透明带宽度(12.84±0.23μm),卵浆直径(117.5±0.70μm),卵母细胞面积(17804±136μm2),卵浆面积(11308.09±75μm);a级和B级卵母细胞的卵浆直径与卵母细胞直径呈正相关(r=0.7792),而C级和D级卵母细胞的卵浆直径与卵母细胞直径呈正相关(r=0.5967)。C级和D级卵母细胞透明带宽度与卵母细胞直径呈中等正相关(r=0.6530),而A级和B级卵母细胞透明带宽度与卵母细胞直径呈中等正相关(r=0.5287)。由此可见,卵浆直径越大,透明带宽度越小,卵母细胞质量越好。因此,为获得较好的发育能力,应加工卵浆较大、透明带较薄的卵母细胞。
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引用次数: 0
GENUS XANTHOPIMPLA SAUSSURE, 1892 (ICHNEUMONIDAE: PIMPLINAE) FROM POTHWAR REGION (PUNJAB) OF PAKISTAN 沙蚤属,1892年,产自巴基斯坦旁遮普省
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.3.0084
S. Ahmed, I. Bodlah, M. F. Nasir, Muhammad Nadeem
Members of Genus Xanthopimpla Saussure, 1892 (Hymenoptera: Ichneumonidae: Pimplinae) are reported as important bio-control agents of different insect pests of various crops. Four species were recorded from Pothwar region with Xanthopimpla reicherti Krieger, 1914 as new record for Pakistan. New distributional records for Xanthopimpla punctata Fabricius, 1781, Xanthopimpla stremmator Thunberg 1822, and Xanthopimpla flavolineata Cameron, 1907 in Pothwar have been added. Comparison of recorded species with closely related species, distribution ranges, and remarks on their host are given. An identification key of recorded species, as well as brief description of each species has been provided, and supplemented with illustrations using microphotography from the external morphological details. Arc GIS software was used for spatial distribution of identified species. Results of these studies including illustrations may be helpful for field workers for identification and researchers to formulate IPM strategies against various insect pests of crops in different districts of Pothwar region of Pakistan.
报道了黄蜂属(Xanthopimpla Saussure, 1892)属昆虫(膜翅目:姬蜂科:小蜂科)是防治多种作物中不同害虫的重要生物制剂。在Pothwar地区记录了4种,其中巴基斯坦的新记录为黄霉(Xanthopimpla reicherti Krieger, 1914)。增加了1781年在Pothwar的斑黄霉(Xanthopimpla punctata Fabricius)、1822年在Pothwar的stremmator Thunberg和1907年在Pothwar的黄霉(Xanthopimpla flavolineata Cameron)的新分布记录。给出了记录种与近缘种的比较、分布范围和对其寄主的评论。提供了所记录物种的识别密钥,以及每个物种的简要描述,并辅以显微摄影的外部形态细节插图。利用Arc GIS软件进行物种空间分布分析。这些研究结果(包括插图)可能有助于田间工作者识别和研究人员制定针对巴基斯坦波特瓦尔地区不同地区作物的各种害虫的IPM策略。
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引用次数: 0
GENETIC DIVERSITY OF ONE CULTIVAR AND 29 GENOTYPES OF TOBACCO BASED ON MORPHOLOGICAL AND YIELD PROPERTIES 基于形态和产量特性的1个烟草品种和29个烟草基因型遗传多样性
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.2.0038
M. Çamlıca, G. Yaldiz
Tobacco (Nicotiana tabacum) is one of the cash and industrial crops commonly grown throughout the world. This plant is important for the agriculture sector in Turkey, with high-quality varieties being produced in different regions of the country. This study aimed to examine important morphological properties and yield of a cultivar and some genotypes of tobacco under the conditions of Bolu province in Turkey in 2015 and 2016. Genetic variability parameters such as GCV (%), PCV (%), GA and heritability were conducted to provide the best image of the tobacco cultivar and genotypes. GCV values were near to PCV values for the properties like DFT, DT and OV indicative high contribution of genotypic effect for phenotypic expression of such properties. These properties had moderately high heritability with 79.98%, 73.95% and 79.51%, respectively. The highest fresh leaf weight was observed in the AR-1 cultivar (525.0 kg da) and ESR-7 genotype (646.30 kg da) in the first year and BSR-3 (358.22 kg da) and ESR-6 (320.19 kg da) genotypes in the second year. A correlation analysis was also conducted between these traits of tobaccos. Highly significant and positive correlations were found between DFT-DT, LL-LW and FLW-DLW. A dendrogram was constructed to determine the relationships between the investigated plants based on morphological properties and yield values. One cultivar and 29 genotypes were located in two main groups as A (14 genotypes and one cultivar) and B (15 genotypes). The genotypes included same region were located in different groups.
烟草(Nicotiana tabacum)是世界上普遍种植的经济和工业作物之一。这种植物对土耳其的农业部门很重要,在该国不同地区生产高质量的品种。本研究旨在研究2015年和2016年土耳其博卢省烟草栽培品种和部分基因型的重要形态特征和产量。利用GCV(%)、PCV(%)、GA和遗传力等遗传变异参数,获得烟草品种和基因型的最佳图像。DFT、DT和OV等性状的GCV值接近PCV值,表明基因型效应对这些性状的表型表达贡献很大。这些性状的遗传率分别为79.98%、73.95%和79.51%。AR-1和ESR-7基因型的鲜叶重在第一年最高(525.0 kg da),第二年最高(358.22 kg da), ESR-6基因型最高(320.19 kg da)。并对这些性状进行了相关分析。DFT-DT、LL-LW和FLW-DLW呈极显著正相关。通过构建树状图来确定所调查植物的形态特征与产量值之间的关系。1个品种和29个基因型分为A(14个基因型和1个品种)和B(15个基因型)两个主要群体。同一区域的基因型分布在不同的组中。
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引用次数: 3
EXAMINATION OF FERTILITY INDICATORS OF EUROPEAN BROWN HARES (Lepus europaeus) IN EASTERN HUNGARY 匈牙利东部欧洲褐兔(Lepus europaeus)生育指标的研究
IF 0.7 4区 生物学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2020-01-01 DOI: 10.36899/japs.2020.3.0075
P. Farkas, S. Kusza, P. Balogh, I. Majzinger
A decreasing tendency in the populations of the European brown hare can be observed in the whole of Europe from the mid-1960s to the present day. Among the reasons for declining populations fertility indicators of females are also mentioned. The aim of our study was to determine the reproductive parameters of the species in eastern Hungary. During the years 2015-2016, we studied two hundred animals (121♀, 79♂). Altogether, 121 uteri (81 adult and 40 juvenile) and 79 testicles were examined. The number of placental scars showed us the number of offspring in reproductive periods. The age of hares was estimated based on the weight of dried eye lens. The number of placental scars in the general linear model was significantly affected by hunting areas, the age of animals, the interaction of the former two, and also by body weight and kidney fat indices. The age of hares, the hunting year, the effect of the interaction of the former two, and body weight seriously contributed to the weight of the testicles. Based on the number of placental scars, 70.37% of adult and 62.50% of juvenile females were proved to be capable of reproduction. One can conclude from the above that although fertility indicators may play an important role in the decreasing trend in the numbers of Hungarian brown hare populations, they are definitely not the main causes.
从20世纪60年代中期到现在,在整个欧洲可以观察到欧洲褐兔种群数量的下降趋势。在人口下降的原因中,还提到了女性生育指标。我们研究的目的是确定该物种在匈牙利东部的生殖参数。在2015-2016年间,我们研究了200只动物(121只母,79只公)。总共检查了121个子宫(81个成人和40个青少年)和79个睾丸。胎盘疤痕的数量向我们展示了生殖期后代的数量。根据干晶状体的重量来估计兔子的年龄。在一般线性模型中,胎盘疤痕数量受狩猎区域、动物年龄、动物年龄与动物年龄的交互作用以及体重和肾脂指数的显著影响。野兔的年龄、狩猎年份、前两者的相互作用和体重的影响,严重影响了睾丸的重量。根据胎盘瘢痕数,70.37%的成虫和62.50%的雌虫具有繁殖能力。从以上可以得出结论,虽然生育指标可能在匈牙利褐兔种群数量减少的趋势中发挥重要作用,但它们绝对不是主要原因。
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引用次数: 0
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Journal of Animal and Plant Sciences
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