Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280115
Nicholas Yakubu Danzima, Peter Mintir Amadu, Rejoice Enyonam Hoedoafia, Maliche Hawa, Vivian Kapio Abem, Samuel Paapa Kofi Paintsil
Background: The goal of this study was to investigate the occurrence and intensity of stress and anxiety among nurses at Tamale Teaching Hospital, along with the factors that contribute to these conditions and the coping mechanisms employed by nurses.
{"title":"The Prevalence and Severity of Psychological Stress and Anxiety among Nurses of Tamale Teaching Hospital-Ghana","authors":"Nicholas Yakubu Danzima, Peter Mintir Amadu, Rejoice Enyonam Hoedoafia, Maliche Hawa, Vivian Kapio Abem, Samuel Paapa Kofi Paintsil","doi":"10.26502/jbb.2642-91280115","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280115","url":null,"abstract":"Background: The goal of this study was to investigate the occurrence and intensity of stress and anxiety among nurses at Tamale Teaching Hospital, along with the factors that contribute to these conditions and the coping mechanisms employed by nurses.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134889757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-02-10DOI: 10.26502/jbb.2642-91280070
Norichika Ogata, Shogo Konishi, Takeshi Yokoyama
Animals depend on other species to live, with monophagy being an extreme mode. Monophagous animals depend on their diet not only for nutritients but also for developmental and reproductive controls. Thus, dietary components may be useful in culturing tissues from monophagous animals. We hypothesized that a dedifferentiated tissue from the monophagous silkworm, Bombyx mori, would re-differentiate when cultured in a medium containing an extract of mulberry (Morus alba) leaves, the only food of B. mori. Over 40 fat-body transcriptomes were sequenced, and we concluded that it is possible to establish in vivo-like silkworm tissue cultures using their diet.
{"title":"<i>In vivo</i>-like Culture of Monophagous Animal Organ using Dietary Components.","authors":"Norichika Ogata, Shogo Konishi, Takeshi Yokoyama","doi":"10.26502/jbb.2642-91280070","DOIUrl":"10.26502/jbb.2642-91280070","url":null,"abstract":"<p><p>Animals depend on other species to live, with monophagy being an extreme mode. Monophagous animals depend on their diet not only for nutritients but also for developmental and reproductive controls. Thus, dietary components may be useful in culturing tissues from monophagous animals. We hypothesized that a dedifferentiated tissue from the monophagous silkworm, <i>Bombyx mori</i>, would re-differentiate when cultured in a medium containing an extract of mulberry (<i>Morus alba</i>) leaves, the only food of <i>B. mori</i>. Over 40 fat-body transcriptomes were sequenced, and we concluded that it is possible to establish <i>in vivo</i>-like silkworm tissue cultures using their diet.</p>","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10845036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280099
Xin Wang, Qiang Fu
Although angiosperms are clearly and strictly defined by their enclosed seeds and enclosed ovules, how old angiosperms are remains controversial. To solve this problem, the only reliable way is digging fossils. The currently widely accepted earliest age for angiosperms is the Early Cretaceous, although this view is facing increasing challenges from pre-Cretaceous fossil evidence of angiosperms as well as molecular clock estimates. Here we report a Palaeozoic angiosperm, Taiyuanostachya gen. nov. This fossil plant has both enclosed ovules and enclosed seeds, features characteristic of angiosperms. Especially, enclosed ovules are idiosyncratic of angiosperms. The occurrences of both characters in Taiyuanostachya declare that angiosperms, the single most diversified plant group on the Earth, have occurred on the Earth in the Palaeozoic, and the origination of angiosperm appears to be much earlier than assumed previously. Although appearing astonishing, this conclusion is in line with the outcomes of molecular clock estimates done decades ago. The discovery of Taiyuanostachya gen. nov is an abominable challenge for botanists who believe groundlessly that angiosperms cannot exist before the Cretaceous.
尽管被子植物被明确而严格地定义为其封闭的种子和封闭的胚珠，但被子植物的年龄仍然存在争议。要解决这个问题，唯一可靠的方法就是挖掘化石。目前被广泛接受的被子植物最早的年龄是早白垩纪，尽管这一观点正面临着来自白垩纪前被子植物化石证据和分子钟估计的越来越多的挑战。本文报道了一株古生代被子植物Taiyuanostachya gen. 11 .，该化石植物具有被子植物的封闭胚珠和封闭种子。特别地，被封闭的胚珠是被子植物的特质。这两个特征的出现，说明被子植物作为地球上最多样化的单一植物类群，早在古生代就已经出现在地球上，被子植物的起源似乎比以前的假设要早得多。尽管看起来令人惊讶，但这一结论与几十年前对分子钟的估计结果一致。对于那些毫无根据地认为被子植物在白垩纪之前不可能存在的植物学家来说，Taiyuanostachya gen. nov的发现是一个可怕的挑战。
{"title":"Taiyuanostachya: An Abominable Angiosperm from the Early Permian of China","authors":"Xin Wang, Qiang Fu","doi":"10.26502/jbb.2642-91280099","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280099","url":null,"abstract":"Although angiosperms are clearly and strictly defined by their enclosed seeds and enclosed ovules, how old angiosperms are remains controversial. To solve this problem, the only reliable way is digging fossils. The currently widely accepted earliest age for angiosperms is the Early Cretaceous, although this view is facing increasing challenges from pre-Cretaceous fossil evidence of angiosperms as well as molecular clock estimates. Here we report a Palaeozoic angiosperm, Taiyuanostachya gen. nov. This fossil plant has both enclosed ovules and enclosed seeds, features characteristic of angiosperms. Especially, enclosed ovules are idiosyncratic of angiosperms. The occurrences of both characters in Taiyuanostachya declare that angiosperms, the single most diversified plant group on the Earth, have occurred on the Earth in the Palaeozoic, and the origination of angiosperm appears to be much earlier than assumed previously. Although appearing astonishing, this conclusion is in line with the outcomes of molecular clock estimates done decades ago. The discovery of Taiyuanostachya gen. nov is an abominable challenge for botanists who believe groundlessly that angiosperms cannot exist before the Cretaceous.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85917616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regulatory T cells (Tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human Tregs are defined as CD4 + CD25 +/high CD127 -/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for Treg profiling, useful for immunomonitoring in clinicals assessment
{"title":"A flow Cytometry Gating Strategy for Profiling CD4+CD25+/highCD127-/low Regulatory T Cells in the Rheumatoid Arthritis Scenario","authors":"Martínez Rosales Ricardo Ulises, Crespo Oliva Celia del Carmen, Chávez Valdés Sheila, Vazquez Arteaga Amalia, Coizeau Rodríguez Edelgis, Ávila Díaz Lismary, Freyre Corrales Giselle, Gonzalez Formental Hany Lianet, Chacón Quintero Yahima, Lemos Perez Gilda","doi":"10.26502/jbb.2642-91280083","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280083","url":null,"abstract":"Regulatory T cells (Tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human Tregs are defined as CD4 + CD25 +/high CD127 -/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for Treg profiling, useful for immunomonitoring in clinicals assessment","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88449662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280071
F. Xu, Zuheng Wang, D. Fu, Xiuquan Shi, Jie Huang, Yuhao Chen, Jianping Da, Tinglin Zhang, Jingping Ge, Xiao-feng Xu, Wen Cheng
. Abstract Background: Urothelial Bladder Cancer (UBC) is one of the most lethal cancers worldwide, the 5-year survival rate remains poor with platinum-based chemotherapy regimens as the standard of cancer treatment protocol. Recent FDA approval of a programmed death ligand-1 (PD-L1) inhibitor, atezolizumab, in advanced UBC patients is changing the therapeutic landscape. Although the response to anti-PD-L1 is correlated to PD-L1 expression and tumor mutation burden, the molecule determinants of responsiveness or non-responsiveness to Immune Checkpoint Inhibitor (ICI) is largely unknown. Methods: R package maftools was used for genomic characterization and differential mutational analysis. EdgeR and DysRegSig algorithm were used for differential gene expression and dysregulator analysis. ConcensusTME algorithm was used for deconvolution of cell types within tumor microenvironment from bulk RNAseq data. Result: A published immunotherapy cohort with whole exome sequencing, RNAseq and clinic outcome data for 29 metastatic urothelial cancer patients was used, paralleled with The Cancer Genome Altas (TCGA) Bladder Cancer cohort,
{"title":"Multi-Omics Analyses Revealed Transcriptional Regulators associated with Immune Checkpoint Inhibitor Treatment in Advanced Bladder Cancer","authors":"F. Xu, Zuheng Wang, D. Fu, Xiuquan Shi, Jie Huang, Yuhao Chen, Jianping Da, Tinglin Zhang, Jingping Ge, Xiao-feng Xu, Wen Cheng","doi":"10.26502/jbb.2642-91280071","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280071","url":null,"abstract":". Abstract Background: Urothelial Bladder Cancer (UBC) is one of the most lethal cancers worldwide, the 5-year survival rate remains poor with platinum-based chemotherapy regimens as the standard of cancer treatment protocol. Recent FDA approval of a programmed death ligand-1 (PD-L1) inhibitor, atezolizumab, in advanced UBC patients is changing the therapeutic landscape. Although the response to anti-PD-L1 is correlated to PD-L1 expression and tumor mutation burden, the molecule determinants of responsiveness or non-responsiveness to Immune Checkpoint Inhibitor (ICI) is largely unknown. Methods: R package maftools was used for genomic characterization and differential mutational analysis. EdgeR and DysRegSig algorithm were used for differential gene expression and dysregulator analysis. ConcensusTME algorithm was used for deconvolution of cell types within tumor microenvironment from bulk RNAseq data. Result: A published immunotherapy cohort with whole exome sequencing, RNAseq and clinic outcome data for 29 metastatic urothelial cancer patients was used, paralleled with The Cancer Genome Altas (TCGA) Bladder Cancer cohort,","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88082368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280090
G. García, Josanne Soto, L. Rodríguez, M. Nuez, N. Dominguez, Emilio F. Buchaca, D. Martínez, Rolando J Gómez, Y. Ávila, Martha R. Carlin, Raúl J. Cano
Objective: The objective of this study is to assess the efficacy of BiotiQuest™ Sugar Shift, a probiotic formulated to transform monosaccharides, rehabilitated human gut microbiota, stabilize HbA1c and blood glucose, improve insulin resistance and reduce inflammation Methods: A double-blind, placebo-controlled study was carried out over 12 weeks with 64 Cubans, aged 30 to 65 with Type 2 Diabetes Mellitus diagnosed, 18 of whom being treated with insulin. Participants were randomly assigned to take either two capsules of the probiotic supplement or of placebo. Clinical measures were evaluated at 28-day intervals, included fasting and post-prandial glucose, HbA1c, lipid panel, insulin, creatinine, and serum lipopolysaccharide levels were assessed. Results: The treated group demonstrated a stabilization in their fasting blood glucose and postprandial glucose levels in treated group respect to the Placebo cohort. The HbA1c levels did not show significant changes in the treated group. The insulin levels decreased significantly in the treated group by day 84 compared to day 1 and to day 84 of the Placebo cohort (p=0.024 and p=0.015, respectively). Serum LPS levels also decreased significantly in the treated group (p=0.001). Conclusions: BiotiQuest™ Sugar Shift is suitable as adjunct therapy for the control of T2D. However, the 12-week trial period was not sufficient to detect significant reductions in all clinical parameters measured and a longer study is recommended.
{"title":"Metabolic Shifting Probiotic in Type 2 Diabetes Mellitus Management: Randomized Clinical Trial","authors":"G. García, Josanne Soto, L. Rodríguez, M. Nuez, N. Dominguez, Emilio F. Buchaca, D. Martínez, Rolando J Gómez, Y. Ávila, Martha R. Carlin, Raúl J. Cano","doi":"10.26502/jbb.2642-91280090","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280090","url":null,"abstract":"Objective: The objective of this study is to assess the efficacy of BiotiQuest™ Sugar Shift, a probiotic formulated to transform monosaccharides, rehabilitated human gut microbiota, stabilize HbA1c and blood glucose, improve insulin resistance and reduce inflammation Methods: A double-blind, placebo-controlled study was carried out over 12 weeks with 64 Cubans, aged 30 to 65 with Type 2 Diabetes Mellitus diagnosed, 18 of whom being treated with insulin. Participants were randomly assigned to take either two capsules of the probiotic supplement or of placebo. Clinical measures were evaluated at 28-day intervals, included fasting and post-prandial glucose, HbA1c, lipid panel, insulin, creatinine, and serum lipopolysaccharide levels were assessed. Results: The treated group demonstrated a stabilization in their fasting blood glucose and postprandial glucose levels in treated group respect to the Placebo cohort. The HbA1c levels did not show significant changes in the treated group. The insulin levels decreased significantly in the treated group by day 84 compared to day 1 and to day 84 of the Placebo cohort (p=0.024 and p=0.015, respectively). Serum LPS levels also decreased significantly in the treated group (p=0.001). Conclusions: BiotiQuest™ Sugar Shift is suitable as adjunct therapy for the control of T2D. However, the 12-week trial period was not sufficient to detect significant reductions in all clinical parameters measured and a longer study is recommended.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83877294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280085
Brentville Va, Vankemmelbeke M, Metheringham Rl, Symonds P
{"title":"SARS-Cov-2 Spike RBD and Nucleocapsid Encoding DNA Vaccine Elicits T cell and Neutralising Antibody Responses that Cross React with Variants","authors":"Brentville Va, Vankemmelbeke M, Metheringham Rl, Symonds P","doi":"10.26502/jbb.2642-91280085","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280085","url":null,"abstract":"","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75909560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280117
Max Bernhard, Bodo Laube
Mammalian neuronal tetrameric ionotropic glutamate receptors (iGluRs) are thought to have originally arisen from the fusion of a bacterial substrate binding protein (SBP) with an inverted potassium channel. This hypothesis is based on structural and sequential similarities between the ligand binding and channel domains of iGluR subunits with SBPs and potassium channels. Ligand binding occurs at the interface between two lobed domains in both ligand binding domains (LBDs) and leads to closure of the shell-like structure, which is considered to be a key element in the transition from ligand recognition to ion channel gating in iGluRs. Here we report the functional coupling of the ectoine-binding protein EhuB to the channel pore of the GluR0 receptor. Fusion of an unmodified EhuB-binding protein to the transmembrane domain of GluR0 did not result in activation of the channel pore. Only by stabilizing the inserted EhuB-binding domain with a dimerization interface the resulting chimera was activated by ectoine, resembling the activation properties of other iGluRs. These results demonstrate the functional compatibility of SBPs to the gate of the channel pore of an iGluR and highlight the role of LBD dimerization in the functional evolution of iGluRs. Based on the high specificity and affinity of SBPs for an incredible variety of substrates, our results demonstrate the competence of SBP/ion channel chimeras for the development of new Biosensors for specific recognition of analytes by functionally linking a bacterial binding protein to the channel pore of an iGluR.
{"title":"Gating Of the Channel Pore of Ionotropic Glutamate Receptors with Bacterial Substrate Binding Proteins: Functional Coupling of the Ectoine Binding Protein Ehub to Glur0","authors":"Max Bernhard, Bodo Laube","doi":"10.26502/jbb.2642-91280117","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280117","url":null,"abstract":"Mammalian neuronal tetrameric ionotropic glutamate receptors (iGluRs) are thought to have originally arisen from the fusion of a bacterial substrate binding protein (SBP) with an inverted potassium channel. This hypothesis is based on structural and sequential similarities between the ligand binding and channel domains of iGluR subunits with SBPs and potassium channels. Ligand binding occurs at the interface between two lobed domains in both ligand binding domains (LBDs) and leads to closure of the shell-like structure, which is considered to be a key element in the transition from ligand recognition to ion channel gating in iGluRs. Here we report the functional coupling of the ectoine-binding protein EhuB to the channel pore of the GluR0 receptor. Fusion of an unmodified EhuB-binding protein to the transmembrane domain of GluR0 did not result in activation of the channel pore. Only by stabilizing the inserted EhuB-binding domain with a dimerization interface the resulting chimera was activated by ectoine, resembling the activation properties of other iGluRs. These results demonstrate the functional compatibility of SBPs to the gate of the channel pore of an iGluR and highlight the role of LBD dimerization in the functional evolution of iGluRs. Based on the high specificity and affinity of SBPs for an incredible variety of substrates, our results demonstrate the competence of SBP/ion channel chimeras for the development of new Biosensors for specific recognition of analytes by functionally linking a bacterial binding protein to the channel pore of an iGluR.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134889763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280095
Guida Cc, Nardella M, Perez Ys A, Recchia A, Tonti Mp, Savino M, Crisetti A, Napolitano F, Ferrara G, Aucella F, Manuali A
Porphyria is a very rare genetic pathology due to congenital defects of heme metabolism with deficiency of one of the enzymes that determine specific forms of porphyria. Acute hepatic porphyrias (AEP) includes four different clinical forms: a) from ALA D deficiency, b) acute intermittent porphyria, c) hereditary coproporphyria and d) variegate porphyria. The forms of porphyria are characterized by elevated levels of delta aminolevulinic acid (ALA) and porphobilinogen (PBG). Acute intermittent porphyria (AIP) is caused by a genetic mutation of hydroxymethylbilane synthetase (HMBS), which in a good percentage (about 20% of cases) does not show a positivity for hepatic porphyria on biomolecular examination, so that diagnosis with relative therapy is determined by the clinical condition. Our study describes a 54-year-old woman who, due to a porphyria crisis, presented serious symptoms with progressive aggravation that lead her to be hospitalized in an intensive care unit where she was intubated due to tetraplegia and severe respiratory failure. Treatment with human emin first and Givosiran subsequently allowed progressive amelioration of the clinical conditions.
卟啉症是一种非常罕见的遗传病理，由于先天性缺陷的血红素代谢缺乏一种酶，决定特定形式的卟啉症。急性肝性卟啉症(AEP)包括四种不同的临床形式:a) ALA D缺乏症，b)急性间歇性卟啉症，c)遗传性比例卟啉症和D)多样性卟啉症。卟啉症的特点是δ氨基乙酰丙酸(ALA)和卟啉胆色素原(PBG)水平升高。急性间断性卟啉症(AIP)是由羟甲基双烷合成酶(HMBS)基因突变引起的，有相当一部分(约20%)的病例在生物分子检查上未表现为肝性卟啉症阳性，因此需要根据临床情况确定是否需要相应的治疗。我们的研究描述了一名54岁的女性，由于卟啉症危象，出现了严重的症状并逐渐加重，导致她因四肢瘫痪和严重呼吸衰竭而住进了重症监护病房，在那里她接受了插管治疗。首先用人emin治疗，随后用吉沃西兰治疗，可使临床状况逐步改善。
{"title":"Genetic Polymorphism in Porphyria: Resolution of A Case of Tetraplegia by Administration of Hemin During a Porphyric Crisis and Subsequent Therapeutic Procedure with the use of Givosiran","authors":"Guida Cc, Nardella M, Perez Ys A, Recchia A, Tonti Mp, Savino M, Crisetti A, Napolitano F, Ferrara G, Aucella F, Manuali A","doi":"10.26502/jbb.2642-91280095","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280095","url":null,"abstract":"Porphyria is a very rare genetic pathology due to congenital defects of heme metabolism with deficiency of one of the enzymes that determine specific forms of porphyria. Acute hepatic porphyrias (AEP) includes four different clinical forms: a) from ALA D deficiency, b) acute intermittent porphyria, c) hereditary coproporphyria and d) variegate porphyria. The forms of porphyria are characterized by elevated levels of delta aminolevulinic acid (ALA) and porphobilinogen (PBG). Acute intermittent porphyria (AIP) is caused by a genetic mutation of hydroxymethylbilane synthetase (HMBS), which in a good percentage (about 20% of cases) does not show a positivity for hepatic porphyria on biomolecular examination, so that diagnosis with relative therapy is determined by the clinical condition. Our study describes a 54-year-old woman who, due to a porphyria crisis, presented serious symptoms with progressive aggravation that lead her to be hospitalized in an intensive care unit where she was intubated due to tetraplegia and severe respiratory failure. Treatment with human emin first and Givosiran subsequently allowed progressive amelioration of the clinical conditions.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78524132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280094
Francisco Aléxis Dntas Maia, Amanda Duarte Godim, Anne Gabriella Dias Santos, Márcio Cleivo de Morais Souza, Vasco de Lima Pinto, Aruzza Mabel de Morais Araújo
This paper presents information about Crambe Abyssinica Hochst and discusses various aspects of oil planting, extraction and treatment, chemical composition, and the physical and chemical properties of the oil. In addition, it discusses the possibility of its use as a raw material for biofuels. Crambe Abyssinica Hochst is an oilseed from the Mediterranean that has good oil content in its seed (~ 38%). Other advantages are presented as the short growth period and can be grown in environments considered unsuitable, which are desirable requirements for use in the production of biofuels. Crambe oil has erucic acid as its main fatty acid, which is not edible, so it is an oil that does not compete with the food industry. This crop is already used in the industry in various parts of the world for the production of various products, including biodiesel with oxidative stability considered promising.
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