Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280086
S. Bito, Tomomi Iioka, Yasuhiro Yamada, E. Hiraoka, Taiju Miyagami
Background: There is lim ited evidence on the effectiveness of decision aids in promoting clinicians' understanding of patients' subjective perceptions. The aim of this study was to assess the impact of using a standard patient-completed template to express patients' views, preferences or concerns to their clinicians in a clinical decision-making setting on patients' perceived decisional conflict and post-decision regret. Methods: A pre-post quasi-experimental study with a six-month control period followed by a 12-month intervention period was conducted. Participants were recruited from six teaching hospitals and two clinics in Japan. The target population included 150 patients with diabetes mellitus, hypertension, and/or dyslipidaemia whose physicians had recently suggested drug treatment as a medical option. In the control period, a general informed consent booklet was distributed, and in the intervention period, a shared decision-making template was also provided. Patients were asked to complete the template, which was then attached to their electronic medical records. Results: Two months after enrolment, the decision conflict and regret scales were mailed to patients. Three months after enrolment, the decision status for starting drug treatment and the concordance between the patients' decision statuses and the initial medical recommendations were observed. Seventy-nine and seventy-one participants assigned to the control and intervention groups were enrolled. Fifty-five patient pairs generated by propensity score matching were analysed. No significant difference was observed between the two groups in the subscale scores of the decision conflict scale and the decision regret scale. The relative risk of patients with a decision status of ‘still considering’ starting drug treatment was 2.2 (95% CI, 1.02-4.9) in the intervention group. The concordance
{"title":"Effect of Handing a Written Document to Share Concerns and Values with Physicians When Making Decisions about Starting Drug Treatment among Patients: A Pre-post Quasi-Experimental Study with Propensity Score Matching","authors":"S. Bito, Tomomi Iioka, Yasuhiro Yamada, E. Hiraoka, Taiju Miyagami","doi":"10.26502/jbb.2642-91280086","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280086","url":null,"abstract":"Background: There is lim ited evidence on the effectiveness of decision aids in promoting clinicians' understanding of patients' subjective perceptions. The aim of this study was to assess the impact of using a standard patient-completed template to express patients' views, preferences or concerns to their clinicians in a clinical decision-making setting on patients' perceived decisional conflict and post-decision regret. Methods: A pre-post quasi-experimental study with a six-month control period followed by a 12-month intervention period was conducted. Participants were recruited from six teaching hospitals and two clinics in Japan. The target population included 150 patients with diabetes mellitus, hypertension, and/or dyslipidaemia whose physicians had recently suggested drug treatment as a medical option. In the control period, a general informed consent booklet was distributed, and in the intervention period, a shared decision-making template was also provided. Patients were asked to complete the template, which was then attached to their electronic medical records. Results: Two months after enrolment, the decision conflict and regret scales were mailed to patients. Three months after enrolment, the decision status for starting drug treatment and the concordance between the patients' decision statuses and the initial medical recommendations were observed. Seventy-nine and seventy-one participants assigned to the control and intervention groups were enrolled. Fifty-five patient pairs generated by propensity score matching were analysed. No significant difference was observed between the two groups in the subscale scores of the decision conflict scale and the decision regret scale. The relative risk of patients with a decision status of ‘still considering’ starting drug treatment was 2.2 (95% CI, 1.02-4.9) in the intervention group. The concordance","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88675126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280074
Walter F. Riesen
Pleiotropic effects are effects not related to the primary effect of drugs. Statins, beside their primary effect, which is LDL-lowering, have several pleiotropic effects, such as anti-inflammation, anti-thrombotic effects, inhibition of smooth muscle cell proliferation and apoptosis, inhibition of migration and activation of macrophages. They increase blood glucose with the exception of pitavastatin. The clinical importance of these pleiotropic effects of statins however remains unclear. No matter of whether LDL-lowering was done by statins or by other means, the lowering of the cardiovascular risk is the same. The reduction in LDL-C per se is responsible for the beneficial effect. However, pleiotropic effects of statins might play a role with respect to micro vascular events. The difference in pleiotropic effects between the different statins might be a basis for a patient-oriented statin therapy.
{"title":"Pleiotropic Effects of Statins-What is their Clinical Significance?","authors":"Walter F. Riesen","doi":"10.26502/jbb.2642-91280074","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280074","url":null,"abstract":"Pleiotropic effects are effects not related to the primary effect of drugs. Statins, beside their primary effect, which is LDL-lowering, have several pleiotropic effects, such as anti-inflammation, anti-thrombotic effects, inhibition of smooth muscle cell proliferation and apoptosis, inhibition of migration and activation of macrophages. They increase blood glucose with the exception of pitavastatin. The clinical importance of these pleiotropic effects of statins however remains unclear. No matter of whether LDL-lowering was done by statins or by other means, the lowering of the cardiovascular risk is the same. The reduction in LDL-C per se is responsible for the beneficial effect. However, pleiotropic effects of statins might play a role with respect to micro vascular events. The difference in pleiotropic effects between the different statins might be a basis for a patient-oriented statin therapy.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"148 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86074379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280087
G. García, D. Martínez, Josanne Soto, L. Rodríguez, M. Nuez
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{"title":"Short-Chain Fructooligosaccharides Improve Gut Microbiota Composition in Patients with Type 2 Diabetes. A randomized, Open-Label, Controlled Pilot Clinical Trial","authors":"G. García, D. Martínez, Josanne Soto, L. Rodríguez, M. Nuez","doi":"10.26502/jbb.2642-91280087","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280087","url":null,"abstract":".","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89516373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280079
Nicholas Yakubu Danzima, Bismark Nantomah, Vivian Kapio Abem
Studies have confirmed that the nurse, during delivery, is the deciding factor whether the woman would have positive or negative birth experience. However, there is no literature on the lived experiences of nursing care rendered during childbirth in the context of Ghana. Hence this study was to discover women’s lived experiences of nursing care during childbirth. A Heideggerian phenomenological method was conducted on 10 women who had given birth. Data analysis was done using Diekermann, Allen and Tanner’s method. Analysis revealed three main themes: (1) Being-in-the-world of pregnant women in labor with emotional, physical, and informational support needs (2) Encounter with the health facility and practices and needing pampering and nice communication (3) Wishing for an environment of congenial and cordial relationship during childbirth. It is recommended that health professionals incorporate the themes emerged from this study into the plan of care for women during childbirth
{"title":"Heideggerian Hermeneutic Phenomenological Study of Lived Experiences of Nursing Care during Childbirth","authors":"Nicholas Yakubu Danzima, Bismark Nantomah, Vivian Kapio Abem","doi":"10.26502/jbb.2642-91280079","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280079","url":null,"abstract":"Studies have confirmed that the nurse, during delivery, is the deciding factor whether the woman would have positive or negative birth experience. However, there is no literature on the lived experiences of nursing care rendered during childbirth in the context of Ghana. Hence this study was to discover women’s lived experiences of nursing care during childbirth. A Heideggerian phenomenological method was conducted on 10 women who had given birth. Data analysis was done using Diekermann, Allen and Tanner’s method. Analysis revealed three main themes: (1) Being-in-the-world of pregnant women in labor with emotional, physical, and informational support needs (2) Encounter with the health facility and practices and needing pampering and nice communication (3) Wishing for an environment of congenial and cordial relationship during childbirth. It is recommended that health professionals incorporate the themes emerged from this study into the plan of care for women during childbirth","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91537134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280110
C Fontaine, S De Brakeleer, E Teugels, V Renard, H Van den Bulck, P Vuylsteke, P. Glorieux, C Dopchie, S Joris, L Decoster, A Awada, K Punie, H Wildiers, J De Grève
Background: BSMO 2014-01 is a published prospective phase 2 study investigating neoadjuvant weekly paclitaxel and carboplatin, followed by epirubicin and cyclophosphamide in 63 patients with triple-negative breast cancer. Pathological complete response (pCR) was 54%. A secondary endpoint was to correlate pCR rate to the presence of germline pathogenic variants in DNA damage response (DDR) genes and in core genes involved in Homologous Recombination (HR).
{"title":"Heterozygote Germline Mutations in Homologous Recombination Core Genes Can Predict for Pathologic Complete Response in Early Triple Negative Breast Cancer","authors":"C Fontaine, S De Brakeleer, E Teugels, V Renard, H Van den Bulck, P Vuylsteke, P. Glorieux, C Dopchie, S Joris, L Decoster, A Awada, K Punie, H Wildiers, J De Grève","doi":"10.26502/jbb.2642-91280110","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280110","url":null,"abstract":"Background: BSMO 2014-01 is a published prospective phase 2 study investigating neoadjuvant weekly paclitaxel and carboplatin, followed by epirubicin and cyclophosphamide in 63 patients with triple-negative breast cancer. Pathological complete response (pCR) was 54%. A secondary endpoint was to correlate pCR rate to the presence of germline pathogenic variants in DNA damage response (DDR) genes and in core genes involved in Homologous Recombination (HR).","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135059430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280106
Godoy-Fernández JF, Cubría-Juárez MP, Zapata-Tarrés M, Taveras-Rodríguez O, Briseño-Rebollar EV
Acute Lymphoblastic Leukemia (ALL) is the most common pediatric cancer and the most common subtype of pediatric leukemia, the high toxicity of chemotherapy, the relapse and refractory disease of many patients require the development of novel therapeutic approaches such as targeted immunotherapy in combination with Hematopoietic Stem Cell Transplantation (HSCT). These new therapeutic approaches must be taken in count to revolutionize the management of numerous ALL subtypes, improve treatment outcomes and maintain low toxicity without losing the effectiveness of the treatment. Our retrospective, longitudinal, observational, descriptive study evaluated a novel therapeutic approach in a Pediatric Oncologic Center in Mexico, the outpatient administration of blinatumomab infusion in patients with relapsed/refractory acute lymphoblastic leukemia (R/R ALL). The study demonstrated that 100% of the patients went into remission after Blinatumomab infusion and there was no mortality associated.
{"title":"Outpatient Administration of Blinatumomab Infusion and Hematopoietic Stem Cell Transplantation as Treatment for Patients with Refractory Acute Lymphoblastic Leukemia in a Pediatric Oncologic Center in Mexico","authors":"Godoy-Fernández JF, Cubría-Juárez MP, Zapata-Tarrés M, Taveras-Rodríguez O, Briseño-Rebollar EV","doi":"10.26502/jbb.2642-91280106","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280106","url":null,"abstract":"Acute Lymphoblastic Leukemia (ALL) is the most common pediatric cancer and the most common subtype of pediatric leukemia, the high toxicity of chemotherapy, the relapse and refractory disease of many patients require the development of novel therapeutic approaches such as targeted immunotherapy in combination with Hematopoietic Stem Cell Transplantation (HSCT). These new therapeutic approaches must be taken in count to revolutionize the management of numerous ALL subtypes, improve treatment outcomes and maintain low toxicity without losing the effectiveness of the treatment. Our retrospective, longitudinal, observational, descriptive study evaluated a novel therapeutic approach in a Pediatric Oncologic Center in Mexico, the outpatient administration of blinatumomab infusion in patients with relapsed/refractory acute lymphoblastic leukemia (R/R ALL). The study demonstrated that 100% of the patients went into remission after Blinatumomab infusion and there was no mortality associated.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135959523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The cost-benefit ratios of the bivalent vaccines were unknown. To address this issue, we considered surveillance data on the administration of the Moderna and Pfizer-BioNTech bivalent vaccines and clinical outcomes from September 1, 2022 to February 10, 2023 for North Carolina residents 12 years of age or older. We found that booster vaccination was much more beneficial for people 65 years of age or older than for people 12–64 years of age, mainly because the risks of hospitalization and death were much higher in the older age group.
{"title":"A Cost-Benefit Analysis of Bivalent Covid-19 Vaccines","authors":"Dan-Yu Lin, Yangjianchen Xu, Donglin Zeng, Shadia Khan Sunny","doi":"10.26502/jbb.2642-91280116","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280116","url":null,"abstract":"The cost-benefit ratios of the bivalent vaccines were unknown. To address this issue, we considered surveillance data on the administration of the Moderna and Pfizer-BioNTech bivalent vaccines and clinical outcomes from September 1, 2022 to February 10, 2023 for North Carolina residents 12 years of age or older. We found that booster vaccination was much more beneficial for people 65 years of age or older than for people 12–64 years of age, mainly because the risks of hospitalization and death were much higher in the older age group.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134889762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280066
Rony Seger, Tamar Hanoch, Revital Rosenberg, Ada Dantes, Wolfgang E Merz, Jerome F Strauss, Abraham Amsterdam
The response of granulosa cells to Luteinizing Hormone (LH) and Follicle- Stimulating Hormone (FSH) is mediated mainly by cAMP/protein kinase A (PKA) signaling. Notably, the activity of the extracellular signal-regulated kinase (ERK) signaling cascade is elevated in response to these stimuli as well. We studied the involvement of the ERK cascade in LH- and FSH-induced steroidogenesis in two granulosa-derived cell lines, rLHR-4 and rFSHR-17, respectively. We found that stimulation of these cells with the appropriate gonadotropin induced ERK activation as well as progesterone production downstream of PKA. Inhibition of ERK activity enhanced gonadotropin-stimulated progesterone production, which was correlated with increased expression of the Steroidogenic Acute Regulatory Protein (StAR), a key regulator of progesterone synthesis. Therefore, it is likely that gonadotropin-stimulated progesterone formation is regulated by a pathway that includes PKA and StAR, and this process is down-regulated by ERK, due to attenuation of StAR expression. Our results suggest that activation of PKA signaling by gonadotropins not only induces steroidogenesis but also activates down-regulation machinery involving the ERK cascade. The activation of ERK by gonadotropins as well as by other agents may be a key mechanism for the modulation of gonadotropin-induced steroidogenesis.
{"title":"The ERK Signaling Cascade Inhibits Gonadotropin-Stimulated Steroidogenesis.","authors":"Rony Seger, Tamar Hanoch, Revital Rosenberg, Ada Dantes, Wolfgang E Merz, Jerome F Strauss, Abraham Amsterdam","doi":"10.26502/jbb.2642-91280066","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280066","url":null,"abstract":"<p><p>The response of granulosa cells to Luteinizing Hormone (LH) and Follicle- Stimulating Hormone (FSH) is mediated mainly by cAMP/protein kinase A (PKA) signaling. Notably, the activity of the extracellular signal-regulated kinase (ERK) signaling cascade is elevated in response to these stimuli as well. We studied the involvement of the ERK cascade in LH- and FSH-induced steroidogenesis in two granulosa-derived cell lines, rLHR-4 and rFSHR-17, respectively. We found that stimulation of these cells with the appropriate gonadotropin induced ERK activation as well as progesterone production downstream of PKA. Inhibition of ERK activity enhanced gonadotropin-stimulated progesterone production, which was correlated with increased expression of the Steroidogenic Acute Regulatory Protein (StAR), a key regulator of progesterone synthesis. Therefore, it is likely that gonadotropin-stimulated progesterone formation is regulated by a pathway that includes PKA and StAR, and this process is down-regulated by ERK, due to attenuation of StAR expression. Our results suggest that activation of PKA signaling by gonadotropins not only induces steroidogenesis but also activates down-regulation machinery involving the ERK cascade. The activation of ERK by gonadotropins as well as by other agents may be a key mechanism for the modulation of gonadotropin-induced steroidogenesis.</p>","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9246460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280088
Biplob Biswas, Wahida Khanam, D. Hossain, Mohammad Ahad Adnan, U. Datta, A. K. Naseruzzaman, Zamil Ahmed Manik, Tauhidul Islam, Kumar Datta, Ahmed Manik
Background: Atopic dermatitis (AD), also called atopic eczema, is a common chronic or recurrent inflammatory skin disease and affects 15-20% of children. Objective: The aim of the study was to determine the proportion of atopy and food allergies among children attending outpatient department of a selected tertiary care hospital in Dhaka city. Methodology: This was a cross-sectional study. It was carried out on 384 children from June 2022 to December 2022 at pediatric outpatient department in Institute of child and mother health (ICMH), Dhaka, Bangladesh. The statistical evaluation of the results by using a window-based computer software program devised with Statistical Packages for Social Sciences (SPSS-24). Result: Total 384 children were enrolled among them Mean age was (1.15±0.43) years, 52% were female and 92.18% had a family history of atopy. Regarding symptoms 4.3% had lip or tongue swelling, 8.5% had difficulty in swallowing, 39.30% had urticaria, 13.7% had dyspnea and 5.1% had anaphylaxis. 82.8% of the respondents had mild illness, 11.98% had moderate illness and 5.21% had severe illness. 48.6% had Cow's Milk allergy, 19.5% to beef, 3.3% to egg ,2.6%to hilsa fish and prawn respectively and 22.3% to others. Mean Eosinophil count was (11.62±2.14), IgE was (747.40±314.2) IU and the skin prick test was positive 90.0%. Conclusion : The predominant sign of food allergy in young children was atopic dermatitis. Allergic conjunctivitis, oral allergy syndrome and respiratory distress appeared later in the progression of symptoms. Additionally, anaphylaxis is usually used to affect older children.
{"title":"Atopy and Food Allergies among Children Attending Outpatient Department of a Selected Tertiary Care Hospital in Dhaka City","authors":"Biplob Biswas, Wahida Khanam, D. Hossain, Mohammad Ahad Adnan, U. Datta, A. K. Naseruzzaman, Zamil Ahmed Manik, Tauhidul Islam, Kumar Datta, Ahmed Manik","doi":"10.26502/jbb.2642-91280088","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280088","url":null,"abstract":"Background: Atopic dermatitis (AD), also called atopic eczema, is a common chronic or recurrent inflammatory skin disease and affects 15-20% of children. Objective: The aim of the study was to determine the proportion of atopy and food allergies among children attending outpatient department of a selected tertiary care hospital in Dhaka city. Methodology: This was a cross-sectional study. It was carried out on 384 children from June 2022 to December 2022 at pediatric outpatient department in Institute of child and mother health (ICMH), Dhaka, Bangladesh. The statistical evaluation of the results by using a window-based computer software program devised with Statistical Packages for Social Sciences (SPSS-24). Result: Total 384 children were enrolled among them Mean age was (1.15±0.43) years, 52% were female and 92.18% had a family history of atopy. Regarding symptoms 4.3% had lip or tongue swelling, 8.5% had difficulty in swallowing, 39.30% had urticaria, 13.7% had dyspnea and 5.1% had anaphylaxis. 82.8% of the respondents had mild illness, 11.98% had moderate illness and 5.21% had severe illness. 48.6% had Cow's Milk allergy, 19.5% to beef, 3.3% to egg ,2.6%to hilsa fish and prawn respectively and 22.3% to others. Mean Eosinophil count was (11.62±2.14), IgE was (747.40±314.2) IU and the skin prick test was positive 90.0%. Conclusion : The predominant sign of food allergy in young children was atopic dermatitis. Allergic conjunctivitis, oral allergy syndrome and respiratory distress appeared later in the progression of symptoms. Additionally, anaphylaxis is usually used to affect older children.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85938524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26502/jbb.2642-91280068
Guodong Luo, AnyuanZhang, L. Chen, Hui Wang, Lan Yu
Streptococcus iniae is a Gram-positive bacterium and a significant pathogen to a wide range of farmed fish. In the present study, we obtained a SF1 derivative, SF1M1, by selecting rifampicin resistance mutants. Compared to the wild type, SF1M1 (i) was slow in growth, (ii) showed less extracellular protease activity and produced a much lower amount of siderophores, and (iii) had a median lethal dose that is more than 100 times higher. In vitro and in vivo studies showed that the attenuated virulence of SF1M1 was stably maintained in the absence of selective pressure. To examine the potential of SF1M1 as a naturally delivered vaccine, Japanese flounder ( Paralichthys olivaceus ) were vaccinated with SF1M1 via bath immersion and oral feeding. At one and two months post-vaccination, the fish were challenged with SF1 or a heterogeneous strain S. iniae 29177, which is of serotype II. The results showed that fish vaccinated with SF1M1 exhibited relative percent survival rates of 54%-70% and produced specific serum antibodies that enhanced complement-mediated bactericidal activity against both SF1 and S. iniae 29177. Taken together, these results indicate that SF1M1 is an effective oral and immersion vaccine that induces protective immunity against serotype I and serotype II S. iniae .
{"title":"Selection of an Attenuated Rifampicin Resistant Mutant of Streptococcus Iniae and Evaluation of its Immunoprotective Effect as Naturally Delivered Vaccines","authors":"Guodong Luo, AnyuanZhang, L. Chen, Hui Wang, Lan Yu","doi":"10.26502/jbb.2642-91280068","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280068","url":null,"abstract":"Streptococcus iniae is a Gram-positive bacterium and a significant pathogen to a wide range of farmed fish. In the present study, we obtained a SF1 derivative, SF1M1, by selecting rifampicin resistance mutants. Compared to the wild type, SF1M1 (i) was slow in growth, (ii) showed less extracellular protease activity and produced a much lower amount of siderophores, and (iii) had a median lethal dose that is more than 100 times higher. In vitro and in vivo studies showed that the attenuated virulence of SF1M1 was stably maintained in the absence of selective pressure. To examine the potential of SF1M1 as a naturally delivered vaccine, Japanese flounder ( Paralichthys olivaceus ) were vaccinated with SF1M1 via bath immersion and oral feeding. At one and two months post-vaccination, the fish were challenged with SF1 or a heterogeneous strain S. iniae 29177, which is of serotype II. The results showed that fish vaccinated with SF1M1 exhibited relative percent survival rates of 54%-70% and produced specific serum antibodies that enhanced complement-mediated bactericidal activity against both SF1 and S. iniae 29177. Taken together, these results indicate that SF1M1 is an effective oral and immersion vaccine that induces protective immunity against serotype I and serotype II S. iniae .","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87633537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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