Single-cell arrays are essential tools for visualizing cellular heterogeneity and for linking cellular phenotypes observed by imaging to intracellular molecular systems through molecular analyses of cells retrieved after imaging. However, conventional single-cell array techniques often suffer from limited applicability due to the narrow microstructures required for single-cell trapping. In this study, we developed a dual-photoresponsive surface that enables single-cell photopatterning without any microstructures, as well as the selective light-induced release of individual cells of interest. The surface is based on a photoclickable material coating, onto which a photocleavable cell-anchoring material is photopatterned for single-cell trapping. We demonstrated light-induced patterning of mammalian immune cells through binding to patterned cell-anchoring materials, enabling microstructure-free single-cell array formation on a flat glass substrate. Furthermore, photopatterned individual cells were selectively released via localized light irradiation and subsequently retrieved at the single-cell level using cell pickers. These results represent the first demonstration of selective cell isolation from photoclickable surfaces following cell patterning and phenotypic observation. This surface is expected to be broadly applicable to a wide range of single-cell analyses by freely tailoring cell-anchoring molecules, designed cell patterns, and downstream molecular analyses.
{"title":"Dual-photoresponsive substrates enabling light-induced single-cell patterning and sorting of nonadherent mammalian cells.","authors":"Xueyang Li, Takahiro Kosaka, Akimitsu Okamoto, Satoshi Yamaguchi","doi":"10.1016/j.jbiosc.2026.01.002","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2026.01.002","url":null,"abstract":"<p><p>Single-cell arrays are essential tools for visualizing cellular heterogeneity and for linking cellular phenotypes observed by imaging to intracellular molecular systems through molecular analyses of cells retrieved after imaging. However, conventional single-cell array techniques often suffer from limited applicability due to the narrow microstructures required for single-cell trapping. In this study, we developed a dual-photoresponsive surface that enables single-cell photopatterning without any microstructures, as well as the selective light-induced release of individual cells of interest. The surface is based on a photoclickable material coating, onto which a photocleavable cell-anchoring material is photopatterned for single-cell trapping. We demonstrated light-induced patterning of mammalian immune cells through binding to patterned cell-anchoring materials, enabling microstructure-free single-cell array formation on a flat glass substrate. Furthermore, photopatterned individual cells were selectively released via localized light irradiation and subsequently retrieved at the single-cell level using cell pickers. These results represent the first demonstration of selective cell isolation from photoclickable surfaces following cell patterning and phenotypic observation. This surface is expected to be broadly applicable to a wide range of single-cell analyses by freely tailoring cell-anchoring molecules, designed cell patterns, and downstream molecular analyses.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-29DOI: 10.1016/j.jbiosc.2026.01.001
Ren Yoshitomi, Shinji Sugiura
Multi-organ microphysiological systems (multi-organ MPSs) are in vitro platforms that simulate interactions between multiple organs in the body. However, most existing multi-organ MPSs often suffer from high complexity and low-throughput due to their reliance on pumps for medium circulation. We have developed a simple multi-organ MPS device, namely interconnected multi-well device, that utilizes a three-dimensional shaker for medium circulation, eliminating the need for pumps. The device consists of two components: a set of four connected cell-culture cups and a series of four interconnected wells. Up to six devices can fit in a standard 24-well plate. The device supports various culture methodologies, including conventional two-dimensional culture and spheroid culture, and is able to accommodate cell culture inserts. Here, we demonstrated the circulation of medium and immune cells throughout the device. Then, as a representative use-case scenario, we demonstrated using the device to evaluate the anticancer effects of the prodrug capecitabine, whose metabolite exhibits anticancer effects, in a two-organ system composed of liver and cancer. In short, our interconnected multi-well device is user-friendly, adaptable to various culture methods, and multi-throughput, and it shows promise for becoming a valuable tool for in vitro organ interaction research.
{"title":"Interconnected multi-well device with three-dimensional shaker-driven culture medium circulation for use as a multi-organ microphysiological system.","authors":"Ren Yoshitomi, Shinji Sugiura","doi":"10.1016/j.jbiosc.2026.01.001","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2026.01.001","url":null,"abstract":"<p><p>Multi-organ microphysiological systems (multi-organ MPSs) are in vitro platforms that simulate interactions between multiple organs in the body. However, most existing multi-organ MPSs often suffer from high complexity and low-throughput due to their reliance on pumps for medium circulation. We have developed a simple multi-organ MPS device, namely interconnected multi-well device, that utilizes a three-dimensional shaker for medium circulation, eliminating the need for pumps. The device consists of two components: a set of four connected cell-culture cups and a series of four interconnected wells. Up to six devices can fit in a standard 24-well plate. The device supports various culture methodologies, including conventional two-dimensional culture and spheroid culture, and is able to accommodate cell culture inserts. Here, we demonstrated the circulation of medium and immune cells throughout the device. Then, as a representative use-case scenario, we demonstrated using the device to evaluate the anticancer effects of the prodrug capecitabine, whose metabolite exhibits anticancer effects, in a two-organ system composed of liver and cancer. In short, our interconnected multi-well device is user-friendly, adaptable to various culture methods, and multi-throughput, and it shows promise for becoming a valuable tool for in vitro organ interaction research.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 1","pages":"Pages 49-57"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146663503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 1","pages":"Pages 30-36"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146663506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 281-289"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 253-259"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 221-229"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 2","pages":"Pages 135-141"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147263339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 158-164"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146669234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 152-157"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146669235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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