{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 1","pages":"Pages 66-73"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146663502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 230-236"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 246-252"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 237-245"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 4","pages":"Pages 300-307"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147100927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 1","pages":"Pages 37-44"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146663505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 194-202"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146669232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 2.9 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 210-220"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146669233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-27DOI: 10.1016/j.jbiosc.2025.12.002
Takamasa Hashizume , Koki Baba , Naoya Matsuo , Bei-Wen Ying
Monoclonal antibodies (mAbs) are key therapeutics for diseases like cancer and autoimmunity. The production of mAbs relies on cell culture, in which the culture medium for high productivity and activity is essential. Despite the traditional manual and advanced computational methodologies for medium optimization, it remains challenging to incorporate biological insights gained during cell culture experimentation into the optimization process. To address this issue, an active learning strategy that sequentially integrates machine learning predictions with experimental observations of biological meaningfulness was developed in the present study. Medium design and prediction were conducted with the combination of the design of experiment and two different machine learning models, to optimize the culture medium for Chinese hamster ovary (CHO) cells producing increased immunoglobulin G (IgG) titer. Using this approach, we iteratively adjusted the concentrations of 44 components in a serum-free medium and achieved a significant improvement in IgG monoclonal antibody production. Biological insights such as osmolality control and amino acid composition, which were not initially considered, were progressively incorporated into the data-driven optimization process. The proposed strategy is practical and effective, even under limited experimental resources, and offers a new direction for rational medium design in biopharmaceutical manufacturing.
{"title":"Sequential active learning for medium optimization in mAb production","authors":"Takamasa Hashizume , Koki Baba , Naoya Matsuo , Bei-Wen Ying","doi":"10.1016/j.jbiosc.2025.12.002","DOIUrl":"10.1016/j.jbiosc.2025.12.002","url":null,"abstract":"<div><div>Monoclonal antibodies (mAbs) are key therapeutics for diseases like cancer and autoimmunity. The production of mAbs relies on cell culture, in which the culture medium for high productivity and activity is essential. Despite the traditional manual and advanced computational methodologies for medium optimization, it remains challenging to incorporate biological insights gained during cell culture experimentation into the optimization process. To address this issue, an active learning strategy that sequentially integrates machine learning predictions with experimental observations of biological meaningfulness was developed in the present study. Medium design and prediction were conducted with the combination of the design of experiment and two different machine learning models, to optimize the culture medium for Chinese hamster ovary (CHO) cells producing increased immunoglobulin G (IgG) titer. Using this approach, we iteratively adjusted the concentrations of 44 components in a serum-free medium and achieved a significant improvement in IgG monoclonal antibody production. Biological insights such as osmolality control and amino acid composition, which were not initially considered, were progressively incorporated into the data-driven optimization process. The proposed strategy is practical and effective, even under limited experimental resources, and offers a new direction for rational medium design in biopharmaceutical manufacturing.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 210-220"},"PeriodicalIF":2.9,"publicationDate":"2025-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-23DOI: 10.1016/j.jbiosc.2025.11.007
Brandhon F. Flores-Ibarra , Andrea I. Enríquez-Rodríguez , Kimberly P. Robles-Pablos , Beatriz A. Rodas-Junco , Waldo M. Argüelles-Monal , Luisa L. Silva-Gutiérrez , Refugio Pérez-González , Olga A. Patrón-Soberano , Carmen S. Rochín-Wong , Luis A. Castillo-Díaz
Type I diabetes is a chronic disease that affects people worldwide. When insulin administration is no longer effective, transplantation of pancreatic islets represents an alternative for diabetics. However, islet grafting carries limitations, which include poor availability of donors, surgery risks, and lifelong immunosuppressive therapy. To address this, novel approaches, such as the use of soft hydrogels as vehicles of cells are being developed for tissue grafting applications. Self-assembling peptide hydrogels (SAPHs) are biocompatible and versatile materials widely used for both, three-dimensional (3D) cell culture and regenerative medicine applications. Therefore, in this study, we explored the effect of the functionalization of the SAPH FEFEFKFKK (FEK9) with extracellular matrix (ECM) motifs, RGD, GFOGER and IKVAV, to support the directed differentiation of human dental pulp stem cells (hDPSCs) into insulin-producing cells (IPCs). The resulting ECM-functionalized FEK9 hydrogel was formed under mildly acidic conditions (pH 5–6). Infrared spectroscopy confirmed that ECM-FEK9 adopts a β-sheet secondary structure and forms a dense nanofibrillar network, while rheological measurements demonstrated the formation of a soft hydrogel. hDPSC cultured in hydrogel displayed steady viability and metabolism. Moreover, under directed induction, cells in ECM-FEK9 expressed β-cell markers, such as PDX-1 and Glut-2, as well as synthetized insulin within 10 days of 3D culture in vitro, as evidenced through fluorescence confocal microscopy and spectrophotometry evaluations, respectively. Therefore, ECM-FEK9 could be a promising candidate to support the culture of hDPSCs and the generation of IPCs after refinement of directed induction under 3D cell culture conditions.
{"title":"Functionalized peptide hydrogel to generate human insulin-producing cells in vitro","authors":"Brandhon F. Flores-Ibarra , Andrea I. Enríquez-Rodríguez , Kimberly P. Robles-Pablos , Beatriz A. Rodas-Junco , Waldo M. Argüelles-Monal , Luisa L. Silva-Gutiérrez , Refugio Pérez-González , Olga A. Patrón-Soberano , Carmen S. Rochín-Wong , Luis A. Castillo-Díaz","doi":"10.1016/j.jbiosc.2025.11.007","DOIUrl":"10.1016/j.jbiosc.2025.11.007","url":null,"abstract":"<div><div>Type I diabetes is a chronic disease that affects people worldwide. When insulin administration is no longer effective, transplantation of pancreatic islets represents an alternative for diabetics. However, islet grafting carries limitations, which include poor availability of donors, surgery risks, and lifelong immunosuppressive therapy. To address this, novel approaches, such as the use of soft hydrogels as vehicles of cells are being developed for tissue grafting applications. Self-assembling peptide hydrogels (SAPHs) are biocompatible and versatile materials widely used for both, three-dimensional (3D) cell culture and regenerative medicine applications. Therefore, in this study, we explored the effect of the functionalization of the SAPH FEFEFKFKK (FEK9) with extracellular matrix (ECM) motifs, RGD, GFOGER and IKVAV, to support the directed differentiation of human dental pulp stem cells (hDPSCs) into insulin-producing cells (IPCs). The resulting ECM-functionalized FEK9 hydrogel was formed under mildly acidic conditions (pH 5–6). Infrared spectroscopy confirmed that ECM-FEK9 adopts a β-sheet secondary structure and forms a dense nanofibrillar network, while rheological measurements demonstrated the formation of a soft hydrogel. hDPSC cultured in hydrogel displayed steady viability and metabolism. Moreover, under directed induction, cells in ECM-FEK9 expressed β-cell markers, such as PDX-1 and Glut-2, as well as synthetized insulin within 10 days of 3D culture <em>in vitro</em>, as evidenced through fluorescence confocal microscopy and spectrophotometry evaluations, respectively. Therefore, ECM-FEK9 could be a promising candidate to support the culture of hDPSCs and the generation of IPCs after refinement of directed induction under 3D cell culture conditions.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 185-193"},"PeriodicalIF":2.9,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145819421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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