Journal of bioscience and bioengineering最新文献_第4页

Selective vanillate production from sugarcane bagasse-derived aromatic compounds using an engineered Pseudomonas sp. NGC7-based strain 利用工程假单胞菌sp. ngc7为基础的菌株从甘蔗渣衍生的芳香化合物中选择性生产香草。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-21 DOI: 10.1016/j.jbiosc.2025.11.001

Md. Jahangir Alam , Naoya Kodama , Kazuma Ikeda , Kanami Muraki , Yudai Higuchi , Masaya Fujita , Naofumi Kamimura , Eiji Masai , Hiroyuki Kurihara , Tomonori Sonoki

In this study, Pseudomonas sp. NGC7 was engineered to selectively produce vanillate (VA) from aromatic compounds in the sugarcane bagasse alkaline extract. A VA O-demethylase gene (vanA4B4)-disrupted strain derived from NGC7 grew and produced VA from the extract containing no saccharides. The organic acids in the extract promoted the strain to grow. The aromatics in the extract were further concentrated by the solid phase extraction with DIAION HP20 resin, and the organic acids were fractionated into the flow-through fraction. A fed-batch culture of NGC7ΔvanA4B4 strain using this concentrated alkaline extract exhibited increased VA production; however, the accumulation of syringate (SA) and 4-hydroxybenzoate (HBA) was also observed along with VA production, despite the strain possessing the genes responsible for SA and HBA degradation. Analysis of the mutants capable of degrading SA while producing VA revealed that mutations in vanR2, a transcriptional repressor of the genes responsible for SA degradation, enabled SA degradation during VA production. In addition, the expression of praI, an HBA hydroxylase derived from Paenibacillus sp. JJ-1b, was suitable for efficient HBA degradation. Thus, the mutation in vanR2 and the expression of praI represented the key engineering strategies for achieving the selective VA production. As the growth of the engineered strains was promoted by the organic acids present in the extract, VA production from the concentrated extract was evaluated in a flow-through-based medium supplemented with mineral salts and metals. Finally, the engineered VA-producing strain produced 4.30 mM VA selectively at a yield of 77 mol% in the practical medium.

本研究对假单胞菌sp. NGC7进行了工程改造，使其能够从甘蔗渣碱性提取物中的芳香化合物中选择性地产生香草酸（VA）。从NGC7衍生的VA o -去甲基化酶基因（vanA4B4）中断菌株生长并从不含糖的提取物中产生VA。提取物中的有机酸促进了菌株的生长。用DIAION HP20树脂固相萃取进一步浓缩提取液中的芳烃，将有机酸分馏成流动馏分。使用该浓缩碱性提取物对NGC7ΔvanA4B4菌株进行分批补料培养，VA产量增加；然而，尽管菌株具有负责SA和HBA降解的基因，但在产生VA的同时，也观察到紫丁香酸（SA）和4-羟基苯甲酸（HBA）的积累。对能够降解SA同时产生VA的突变体的分析表明，vanR2（负责SA降解的基因的转录抑制因子）的突变使SA在VA产生过程中降解。此外，源自Paenibacillus sp. JJ-1b的HBA羟化酶praI的表达适合于HBA的高效降解。因此，vanR2的突变和praI的表达代表了实现选择性VA产生的关键工程策略。由于提取物中存在的有机酸促进了工程菌株的生长，因此在添加无机盐和金属的流动培养基中评估了浓缩提取物的VA产量。最后，该工程产VA菌株在实际培养基中选择性地产生4.30 mM VA，产率为77 mol%。

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引用次数: 0

One-pot synthesis of β-alanine from 1,3-diaminopropane using two-enzyme cascade in cell-free biotransformation 用双酶级联法在无细胞生物转化中一锅法合成1,3-二氨基丙烷β-丙氨酸。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-17 DOI: 10.1016/j.jbiosc.2025.10.013

Shanthi Shanmugasundaram, Anitha Janet Roshni Yesudhas, Andrea Kagoo, Ramalingam Subramanian

A novel two-enzyme cascade one-pot synthesis of β-alanine from 1,3-diaminopropane (DAP) has been developed. In the first step, DAP was oxidized to 3-aminopropionaldehyde (3-APAL) by diamine oxidase (DAO). In the second step, 3-APAL was oxidized to β-alanine by 3-APAL dehydrogenase (APALDH). Catalase and NADH oxidase were employed to degrade the by-product, H₂O₂, and to regenerate the cofactor, NAD⁺. DAO specific to DAP has not been reported in any prokaryote. Therefore, initial proof of concept was established using commercial eukaryotic DAO_pk (from porcine kidney) and catalase (from bovine liver), along with recombinant APALDH and NOX enzymes sourced from Arthrobacter crystallopoietes and Lactococcus lactis, respectively. β-Alanine was successfully produced via this pathway; 12.2 mM (1.1 g/L) was formed in 41 h with 12 % conversion. To increase the reaction rate, DAO with higher specific activity was identified from Arthrobacter pascens (DAO_Ap). The optimum pH and temperature of DAO_Ap were determined to be 9.0 and 37 °C, respectively. Batch enzymatic biotransformation achieved 6 % conversion, yielding 0.33 mM (29 mg/L) β-alanine in 4 h. The low titre in batch conversion was attributed to substrate inhibition affecting DAO_Ap, NOX, and catalase. Fed-batch enzymatic biotransformation was conducted to overcome substrate inhibition, yielding 47 % conversion, with 2.34 mM (63 mg/L) β-alanine formation in 4 h. Approximately a 7.5-fold increase in conversion was achieved using fed-batch enzymatic biotransformation. This study accomplished a novel two-enzyme cascade biotransformation strategy for one-pot β-alanine synthesis from DAP.

以1,3-二氨基丙烷（DAP）为原料，采用双酶级联一锅法合成β-丙氨酸。第一步，二胺氧化酶（DAO）将DAP氧化为3-氨基丙醛（3-APAL）。第二步，3-APAL脱氢酶（APALDH）将3-APAL氧化为β-丙氨酸。过氧化氢酶和NADH氧化酶被用来降解副产物H2O2，并再生辅助因子NAD+。在任何原核生物中未见DAP特异性DAO的报道。因此，最初的概念证明是使用商业真核DAOpk（来自猪肾）和过氧化氢酶（来自牛肝脏），以及分别来自节杆菌晶体和乳酸球菌的重组APALDH和NOX酶建立的。β-丙氨酸通过该途径成功生成；以12%的转化率，在41 h内形成12.2 mM （1.1 g/L）。为了提高反应速率，从pascens节杆菌（Arthrobacter pascens, DAOAp）中鉴定出具有较高比活性的DAO。测定了DAOAp的最佳pH为9.0℃，最佳温度为37℃。批量酶生物转化达到6%的转化率，在4小时内产生0.33 mM (29 mg/L) β-丙氨酸。批量转化的低滴度归因于底物对DAOAp、NOX和过氧化氢酶的抑制作用。为了克服底物抑制，进行了补料批式酶生物转化，转化率为47%，在4小时内生成2.34 mM (63 mg/L) β-丙氨酸。使用补料批式酶生物转化，转化率提高了约7.5倍。本研究完成了一种新的双酶级联生物转化策略，用于一锅法合成β-丙氨酸。

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引用次数: 0

Biodegradation of recalcitrant environmental pollutants by white-rot fungi 白腐菌降解难降解环境污染物的研究。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-17 DOI: 10.1016/j.jbiosc.2025.10.010

Mingdong Chang , Ru Yin , Jianqiao Wang , Nana Wang , Pengfei Xiao

The understanding of white-rot fungi (WRF) and their role in degrading recalcitrant environmental pollutants has significantly advanced due to developments in bioremediation research. Considerable progress has been made in elucidating the degradation capabilities of WRF against lots of environmental pollutants. In this review, research hotspots on the degradation of WRF were identified through bibliometric analysis. Key findings from systematic studies on the degradation of polycyclic aromatic hydrocarbons (PAHs) and bisphenols by WRF are synthesized and discussed. Furthermore, insights into the molecular and genetic basis underlying the enzymatic systems responsible for the degradation of PAHs and bisphenols are highlighted. Advancements and challenges in understanding the degradation capabilities and degradation mechanisms are examined in order to identify opportunities for developing more effective strategies to harness the bioremediation potential of WRF.

由于生物修复研究的发展，人们对白腐菌及其在降解难降解环境污染物中的作用的了解取得了显著进展。WRF对多种环境污染物的降解能力的研究取得了长足的进展。本文通过文献计量学分析，确定了WRF降解的研究热点。综述了WRF降解多环芳烃（PAHs）和双酚类化合物的系统研究成果。此外，深入了解的分子和遗传基础背后的酶系统负责降解多环芳烃和双酚被强调。研究了在理解降解能力和降解机制方面取得的进展和面临的挑战，以确定制定更有效的战略以利用WRF的生物修复潜力的机会。

引用次数: 0

Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation pseudoncardidia sp. D17对顺式-1,2-二氯乙烯的好氧降解特性：降解动力学，假定的降解途径，以及可溶性二铁单加氧酶在初始氧化中的作用

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-12 DOI: 10.1016/j.jbiosc.2025.10.012

Ryugo Nishimine , Yuna Kaneko , Shinpei Fujiwara , Daisuke Inoue , Masahiro Takeo , Michihiko Ike

Pseudonocardia sp. D17 (D17) is a novel strain capable of aerobically metabolizing cis-1,2-dichloroethene (cDCE), a persistent contaminant in soil and groundwater. This study aimed to investigate the cDCE degradation characteristics of D17 with respect to kinetics, associated degradative enzymes, and degradation pathways. Degradation experiments with cDCE concentrations ranging from 0.267 to 91.3 μM revealed that D17 can efficiently degrade cDCE across this range. The maximum specific degradation rate and half saturation constant for cDCE degradation by D17 were estimated to be 0.418 ± 0.045 nmol/mg-protein/min and 38.5 ± 9.2 μM, respectively. Heterologous expression experiments demonstrated that both group 5 soluble di-iron monooxygenases in D17, namely tetrahydrofuran and propane monooxygenases, can catalyze cDCE degradation with higher catalytic activity observed in the former. This suggests their involvement in cDCE degradation by D17. It was also proposed that D17 completely dechlorinates cDCE through multiple pathways to generate glyoxylic acid, which is either mineralized or incorporated into the glyoxylate cycle, with a minor portion being converted to oxalic acid as a dead-end product. These findings provide novel insights into metabolic aerobic cDCE biodegradation and highlight the potential of D17 as a bioremediation agent.

伪心藻sp. D17 （D17）是一种能够代谢土壤和地下水中持久性污染物顺-1,2-二氯乙烯（cDCE）的新型菌株。本研究旨在从动力学、相关降解酶和降解途径等方面探讨D17对cDCE的降解特性。在0.267 ~ 91.3 μM的cDCE浓度范围内，D17能有效降解cDCE。D17降解cDCE的最大特定降解速率和半饱和常数分别为0.418±0.045 nmol/mg-protein/min和38.5±9.2 μM。异源表达实验表明，D17中第5组可溶性二铁单加氧酶，即四氢呋喃单加氧酶和丙烷单加氧酶都能催化cDCE降解，且前者的催化活性更高。这表明它们参与了D17对cDCE的降解。也有人提出，D17通过多种途径使cDCE完全脱氯生成乙醛酸，乙醛酸要么矿化，要么并入乙醛酸循环，一小部分作为死端产物转化为草酸。这些发现为代谢有氧cDCE生物降解提供了新的见解，并突出了D17作为生物修复剂的潜力。

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引用次数: 0

Breeding bafilomycin A1-resistant sake yeast to improve fermentative capacity 选育抗巴霉素a1型清酒酵母提高发酵能力。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-12 DOI: 10.1016/j.jbiosc.2025.10.006

Mai Nakase , Hiroyuki Senjyu , Takuya Asai , Kaoru Takegawa , Takahiro Akashi

Sake yeast exhibits remarkable fermentative capacity in mash environments, even under various stress conditions. Although breeding techniques aimed at improving the flavor of sake by modifying aroma compounds and organic acid composition have been employed, these approaches often result in reduced fermentative capacity. Furthermore, existing breeding methods aimed at enhancing fermentative capacity often result in increased acidity in the final product. In this study, we aimed to improve sake yeast fermentative capacity while limiting sake acidity. Depletion of intracellular ATP may enhance fermentative capacity, suggesting that strains with high vacuolar-type ATPase (V-ATPase) activity exhibit improved fermentative capacity. Thus, we subjected sake yeast to ultraviolet mutagenesis and bafilomycin A1 (Baf), a V-ATPase inhibitor, to select resistant strains. The selected Baf-resistant strains exhibited no changes in growth rate, cell morphology, or vacuolar morphology compared to the parent strain. However, increased vacuolar acidity and decreased intracellular ATP levels indicated enhanced V-ATPase activity. Moreover, evaluation of brewing characteristics confirmed improved fermentative capacity without increases in acidity or amino acid content. The results of this study suggest that obtaining a Baf-resistant strain can reduce intracellular ATP levels, thereby increasing fermentative capacity without increasing acidity.

清酒酵母在醪环境中表现出显著的发酵能力，即使在各种应激条件下也是如此。虽然育种技术旨在通过改变香气化合物和有机酸组成来改善清酒的风味，但这些方法往往导致发酵能力降低。此外，现有的旨在提高发酵能力的育种方法往往导致最终产品的酸度增加。本研究旨在提高清酒酵母菌的发酵能力，同时限制清酒的酸度。细胞内ATP的消耗可能会增加发酵能力，这表明具有高液泡型ATP酶（v -ATP酶）活性的菌株具有更好的发酵能力。因此，我们对清酒酵母进行紫外诱变和v - atp酶抑制剂巴菲霉素A1 （Baf）筛选耐药菌株。与亲本菌株相比，所选的抗baf菌株在生长速度、细胞形态或液泡形态方面没有变化。然而，空泡酸度增加和细胞内ATP水平降低表明v -ATP酶活性增强。此外，酿造特性的评价证实，在没有增加酸度或氨基酸含量的情况下，发酵能力得到了改善。本研究结果表明，获得抗baf菌株可以降低细胞内ATP水平，从而在不增加酸度的情况下提高发酵能力。

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引用次数: 0

Production of fucoxanthin using a water surface-floating microalga Chromophyton sp. (Hikarimo) 利用浮在水面的微藻色藻sp. （Hikarimo）生产岩藻黄素。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-11 DOI: 10.1016/j.jbiosc.2025.10.009

Akira Umehara , Mardhiah Wahab , Kohei Yoneda , Fazrena Nadia Md Akhir , Koji Iwamoto , Ken-ichiro Ishida , Satomi Hiruta , Masahiro Suzuki , Izzah Nadzirah Rushdan Zaim , Tomoharu Sano , Masanobu Kawachi , Iwane Suzuki , Yoshiaki Maeda

Water-surface floating microalgae have a potential to be promising host organisms to produce useful compounds free from energy-consuming and costly harvesting processes. However, only a few water-surface floating microalgae have been studied for biotechnological applications. In this study, we investigated the potential of a chrysophycean alga Chromophyton sp., also known as Hikarimo in Japan, as a producer of fucoxanthin. The cells of this microalga float on the surface of freshwater in natural environments and can be harvested by attaching plastic film to the floating cells. Three strains, SH01, SH02, and SH03, were isolated from Japanese freshwater environments in Ibaraki, Nagano, and Chiba, and all three strains were identified as Chromophyton sp. by molecular phylogenetic analysis. After we prepared a less contaminated culture of these strains, culture conditions, namely medium concentrations, temperatures, and photon flux densities, were optimized to enhance the cell concentrations. As a result, the cell concentration of Chromophyton sp. reached 16.7 × 10⁶ cells/ml, which is 10.7 times higher than that before the investigation. The cultured cells did not show a water-surface floating phenotype, and thus, we should identify the trigger(s) to induce this floating phenotype. High-performance liquid chromatography analyses revealed that Chromophyton sp. was rich in fucoxanthin. The fucoxanthin content and productivity were estimated to be 48.7 mg/g of dry cell weight and 2.31 mg/L/day, respectively. This is the highest level among the microalgal species studied so far. Since Chromophyton sp. was observed to be free from a cell wall, this microalga would also be favorable for food and feed applications.

水面漂浮的微藻有潜力成为有前途的寄主生物，可以产生有用的化合物，而不需要消耗能量和昂贵的收集过程。然而，只有少数水面漂浮微藻被研究用于生物技术应用。在这项研究中，我们研究了一种chrysophysea藻类Chromophyton sp.，在日本也被称为Hikarimo，作为岩藻黄素的生产者的潜力。这种微藻的细胞漂浮在自然环境的淡水表面，可以通过将塑料薄膜附着在漂浮的细胞上来收获。从日本茨城、长野和千叶的淡水环境中分离到3株菌株SH01、SH02和SH03，经分子系统发育分析，3株菌株均为色藻属。在制备了这些菌株的低污染培养物后，我们优化了培养条件，即培养基浓度、温度和光子通量密度，以提高细胞浓度。实验结果显示，染色菌的细胞浓度达到16.7 × 106个/ml，比实验前提高了10.7倍。培养的细胞没有表现出水面漂浮表型，因此，我们应该确定诱导这种漂浮表型的触发因素。高效液相色谱分析表明，该菌富含岩藻黄质。岩藻黄质含量为48.7 mg/g，产量为2.31 mg/L/d。这是迄今为止所研究的微藻物种中最高的水平。由于观察到色藻没有细胞壁，这种微藻也有利于食品和饲料的应用。

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引用次数: 0

Aspergillus oryzae strains heterogeneously produce plant polysaccharide degradation-related enzymes 米曲霉菌株异质产生植物多糖降解相关酶。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-10 DOI: 10.1016/j.jbiosc.2025.10.008

Shimma Fujiwa , Ryousuke Kataoka , Kazuhiro Iwashita , Tomohiko Matsuzawa

Aspergillus oryzae is used for brewing, and many strains with different brewing characteristics have been isolated. We compared enzymatic activities of eight A. oryzae strains, RIB40, 128, 143, 163, 301, 915, 1108, and 1178 strains, toward plant polysaccharides. The plant polysaccharide degradation-related enzymes produced by A. oryzae change depending on the monosaccharides and polysaccharides added to the culture medium. RIB915 produced more cellulolytic and hemicellulolytic enzymes, whereas RIB40, 128, and 301 produced less of these enzymes than the other strains. In addition, A. oryzae RIB128 produced different glycoside hydrolases in response to monosaccharides and polysaccharides compared with other strains. These results indicated that there is diversity in the production of plant polysaccharide degradation-related enzymes within A. oryzae species.

米曲霉用于酿造，已分离出许多具有不同酿造特性的菌株。我们比较了8株稻瘟病菌RIB40、128、143、163、301、915、1108和1178菌株对植物多糖的酶活性。m.o ryzae产生的植物多糖降解相关酶随着培养基中添加的单糖和多糖的不同而变化。与其他菌株相比，RIB915产生更多的纤维素分解酶和半纤维素分解酶，而RIB40、128和301产生的这些酶较少。此外，与其他菌株相比，A. oryzae RIB128对单糖和多糖产生不同的糖苷水解酶。这些结果表明，米芽孢杆菌在植物多糖降解相关酶的生产中存在多样性。

引用次数: 0

Production of oxygen-generating collagen gel and quantitative analysis of oxygen generation ability 产氧胶原凝胶的制备及产氧能力的定量分析。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-04 DOI: 10.1016/j.jbiosc.2025.10.007

Tengchang Li , Toshihisa Kajiwara , Hiroshi Mizumoto

Tissue engineering of thick hepatic tissues is limited by an inadequate oxygen supply, which causes hypoxia and cell death. Oxygen-generating materials have emerged to temporarily relieve hypoxia. However, quantitative analysis of their ability to generate oxygen is still lacking, hindering the precise evaluation of their efficacy. In this study, we developed an oxygen-generating collagen gel (oxy CG) containing calcium peroxide (CaO₂) and quantitatively analyzed its oxygen release dynamics by measuring the oxygen generation rate and calculating its volumetric oxygen transfer coefficient (k_La). Using this method, we derived a theoretical threshold for cultivable cell density that was experimentally validated under hypoxic conditions using primary rat hepatocytes (PRHs). Oxy CG sustained oxygen release, while maintaining pH stability, supporting hepatocyte viability and liver-specific functions, such as urea synthesis and albumin secretion, within the predicted cell density range. This study provides a quantitative framework that balances oxygen supply and cellular demand, providing valuable insights for optimizing oxygen delivery in liver tissue engineering to overcome diffusion limitations in 3D constructs and improve clinical applicability.

厚肝组织的组织工程受到氧气供应不足的限制，导致缺氧和细胞死亡。产生氧气的材料已经出现，可以暂时缓解缺氧。然而，对其产氧能力的定量分析仍然缺乏，阻碍了对其功效的精确评估。本研究研制了一种含氧化钙（CaO2）的产氧胶原凝胶（oxy CG），并通过测量其产氧速率和计算其体积氧传递系数（kLa），定量分析了其氧释放动力学。利用这种方法，我们推导出了一个理论阈值的可培养细胞密度，并在缺氧条件下用原代大鼠肝细胞（PRHs）进行了实验验证。Oxy CG在维持pH稳定的同时持续释放氧气，支持肝细胞活力和肝脏特异性功能，如尿素合成和白蛋白分泌，在预测的细胞密度范围内。本研究提供了平衡氧气供应和细胞需求的定量框架，为优化肝组织工程中的氧气输送提供了有价值的见解，以克服3D构建中的扩散限制，提高临床适用性。

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引用次数: 0

Production of 1-(β-d-ribofuranosyl)-1,2,4-triazole through solid-state fermentation with Purpureocillium lavendulum DQWM-G4 紫紫色紫霉DQWM-G4固态发酵生产1-（β-d-核呋喃基）- 1,2,4-三唑

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-11-01 DOI: 10.1016/j.jbiosc.2025.10.005

Jian-Wei Dong, Meng Liu, Xue-Jiao Li, Ke-Wen Pan

1-(β-d-Ribofuranosyl)-1,2,4-triazole (RTA) is a nucleotide analog of 1,2,4-triazole that demonstrates broad-spectrum antiviral activity. It is currently the approved drug for treating chronic hepatitis E virus (HEV) infections. However, the production of RTA relies predominantly on chemical synthesis and extraction from plants and animals, with no report of microbial metabolic production. This study introduces a novel approach to producing RTA via solid-state fermentation (SSF) using Purpureocillium lavendulum DQWM-G4, with rice as the substrate. The chemical structure of RTA was elucidated through ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy. Fermentation conditions for producing RTA were optimized by investigating substrate type, temperature, and duration. Under optimized conditions, SSF with P. lavendulum DQWM-G4 on rice at 20 °C for 60 d yielded RTA at a high concentration of 3.46 ± 0.16 mg/g. This paper represents the first report on microbial production of RTA, offering an alternative to chemical synthesis and natural extraction.

1-（β-d-核糖呋喃基）-1,2,4-三唑（RTA）是1,2,4-三唑的核苷酸类似物，具有广谱抗病毒活性。它是目前批准用于治疗慢性戊型肝炎病毒（HEV）感染的药物。然而，RTA的生产主要依赖于化学合成和从植物和动物中提取，没有微生物代谢生产的报道。本研究介绍了一种以水稻为底物，利用紫紫色毛霉DQWM-G4固态发酵生产RTA的新方法。通过1H和13C核磁共振（NMR）谱分析了RTA的化学结构。通过考察底物类型、发酵温度和发酵时间，优化了RTA的发酵条件。在优化条件下，黄花苜蓿DQWM-G4的SSF在水稻上20℃作用60 d， RTA浓度为3.46±0.16 mg/g。本文首次报道了微生物生产RTA，为化学合成和天然提取提供了一种替代方法。

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引用次数: 0

Lipid metabolism prediction in oleaginous yeasts across taxonomic levels using single-cell innate fluorescence signature 利用单细胞先天荧光标记预测产油酵母在不同分类水平上的脂质代谢。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-10-31 DOI: 10.1016/j.jbiosc.2025.10.003

Shiomi Yawata , Tomohiro Hirayama , Tatsuro Serita , Haruka Kazama , Koji Mori , Hiroaki Takaku , Nobuhiko Nomura , Yutaka Yawata

Cellular innate fluorescence (IF) is a natural fluorescence derived from cellular metabolites and biomolecules within microbial cells. Although IF is suggested to be a promising tool for probing the physiology of cells in a non-invasive manner, the link between single-cell IF and heterogeneity in material production remains largely unexplored. This study aimed to examine the link between cellular IF in oleaginous yeasts and their lipid-producing capabilities at multiple taxonomic levels: intra-species, inter-species, and inter-genus. Briefly, we developed a novel microscopic method that can directly link cellular IF (a single-cell IF signature) and the lipid-producing capability of cells at single-cell resolution, thereby enabling the recognition of high heterogeneity in single-cell IF and lipid production in cells. At the intra-species level, the time-course analysis revealed a parallelism between the shifts in single-cell IF signatures and lipid production by Lipomyces starkeyi. The regression model constructed based on single-cell IF signatures could predict intracellular lipid contents. The link between IF and lipid production was also observed across the inter-strain, inter-species, and inter-genus levels, where the regression model constructed with single-cell IF signatures of different strains, species, and genera could predict lipid production. Machine learning models established a computational link to predict lipid productivity by relying on the single-cell IF signatures. These results indicate that the single-cell IF signature is a promising tool for lipid production analysis and prediction in oleaginous yeast species across taxa.

细胞固有荧光（IF）是微生物细胞内代谢产物和生物分子产生的一种天然荧光。虽然中频被认为是一种很有前途的工具，可以以非侵入性的方式探测细胞的生理机能，但单细胞中频与物质生产异质性之间的联系在很大程度上仍未被探索。本研究旨在从种内、种间和属间等多个分类水平上研究产油酵母细胞IF与其产脂能力之间的联系。简而言之，我们开发了一种新的显微镜方法，可以直接将细胞中频（单细胞中频特征）与细胞在单细胞分辨率下的脂质产生能力联系起来，从而能够识别单细胞中频和细胞中脂质产生的高度异质性。在种内水平上，时间过程分析揭示了单细胞IF特征的变化与starkeyi脂质产生之间的平行性。基于单细胞IF特征构建的回归模型可以预测细胞内脂质含量。在菌株间、种间和属间水平上也观察到IF与脂质产生之间的联系，其中利用不同菌株、种和属的单细胞IF特征构建的回归模型可以预测脂质产生。机器学习模型建立了一个计算链接，通过依赖单细胞IF特征来预测脂质生产力。这些结果表明，单细胞IF标记是一种很有前途的工具，可以用于分析和预测产油酵母在不同分类群中的产脂过程。

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引用次数: 0