Journal of bioscience and bioengineering最新文献_第4页

Production of fucoxanthin using a water surface-floating microalga Chromophyton sp. (Hikarimo) 利用浮在水面的微藻色藻sp. （Hikarimo）生产岩藻黄素。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-11-11 DOI: 10.1016/j.jbiosc.2025.10.009

Akira Umehara , Mardhiah Wahab , Kohei Yoneda , Fazrena Nadia Md Akhir , Koji Iwamoto , Ken-ichiro Ishida , Satomi Hiruta , Masahiro Suzuki , Izzah Nadzirah Rushdan Zaim , Tomoharu Sano , Masanobu Kawachi , Iwane Suzuki , Yoshiaki Maeda

Water-surface floating microalgae have a potential to be promising host organisms to produce useful compounds free from energy-consuming and costly harvesting processes. However, only a few water-surface floating microalgae have been studied for biotechnological applications. In this study, we investigated the potential of a chrysophycean alga Chromophyton sp., also known as Hikarimo in Japan, as a producer of fucoxanthin. The cells of this microalga float on the surface of freshwater in natural environments and can be harvested by attaching plastic film to the floating cells. Three strains, SH01, SH02, and SH03, were isolated from Japanese freshwater environments in Ibaraki, Nagano, and Chiba, and all three strains were identified as Chromophyton sp. by molecular phylogenetic analysis. After we prepared a less contaminated culture of these strains, culture conditions, namely medium concentrations, temperatures, and photon flux densities, were optimized to enhance the cell concentrations. As a result, the cell concentration of Chromophyton sp. reached 16.7 × 10⁶ cells/ml, which is 10.7 times higher than that before the investigation. The cultured cells did not show a water-surface floating phenotype, and thus, we should identify the trigger(s) to induce this floating phenotype. High-performance liquid chromatography analyses revealed that Chromophyton sp. was rich in fucoxanthin. The fucoxanthin content and productivity were estimated to be 48.7 mg/g of dry cell weight and 2.31 mg/L/day, respectively. This is the highest level among the microalgal species studied so far. Since Chromophyton sp. was observed to be free from a cell wall, this microalga would also be favorable for food and feed applications.

水面漂浮的微藻有潜力成为有前途的寄主生物，可以产生有用的化合物，而不需要消耗能量和昂贵的收集过程。然而，只有少数水面漂浮微藻被研究用于生物技术应用。在这项研究中，我们研究了一种chrysophysea藻类Chromophyton sp.，在日本也被称为Hikarimo，作为岩藻黄素的生产者的潜力。这种微藻的细胞漂浮在自然环境的淡水表面，可以通过将塑料薄膜附着在漂浮的细胞上来收获。从日本茨城、长野和千叶的淡水环境中分离到3株菌株SH01、SH02和SH03，经分子系统发育分析，3株菌株均为色藻属。在制备了这些菌株的低污染培养物后，我们优化了培养条件，即培养基浓度、温度和光子通量密度，以提高细胞浓度。实验结果显示，染色菌的细胞浓度达到16.7 × 106个/ml，比实验前提高了10.7倍。培养的细胞没有表现出水面漂浮表型，因此，我们应该确定诱导这种漂浮表型的触发因素。高效液相色谱分析表明，该菌富含岩藻黄质。岩藻黄质含量为48.7 mg/g，产量为2.31 mg/L/d。这是迄今为止所研究的微藻物种中最高的水平。由于观察到色藻没有细胞壁，这种微藻也有利于食品和饲料的应用。

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引用次数: 0

Production of oxygen-generating collagen gel and quantitative analysis of oxygen generation ability 产氧胶原凝胶的制备及产氧能力的定量分析。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-11-04 DOI: 10.1016/j.jbiosc.2025.10.007

Tengchang Li , Toshihisa Kajiwara , Hiroshi Mizumoto

Tissue engineering of thick hepatic tissues is limited by an inadequate oxygen supply, which causes hypoxia and cell death. Oxygen-generating materials have emerged to temporarily relieve hypoxia. However, quantitative analysis of their ability to generate oxygen is still lacking, hindering the precise evaluation of their efficacy. In this study, we developed an oxygen-generating collagen gel (oxy CG) containing calcium peroxide (CaO₂) and quantitatively analyzed its oxygen release dynamics by measuring the oxygen generation rate and calculating its volumetric oxygen transfer coefficient (k_La). Using this method, we derived a theoretical threshold for cultivable cell density that was experimentally validated under hypoxic conditions using primary rat hepatocytes (PRHs). Oxy CG sustained oxygen release, while maintaining pH stability, supporting hepatocyte viability and liver-specific functions, such as urea synthesis and albumin secretion, within the predicted cell density range. This study provides a quantitative framework that balances oxygen supply and cellular demand, providing valuable insights for optimizing oxygen delivery in liver tissue engineering to overcome diffusion limitations in 3D constructs and improve clinical applicability.

厚肝组织的组织工程受到氧气供应不足的限制，导致缺氧和细胞死亡。产生氧气的材料已经出现，可以暂时缓解缺氧。然而，对其产氧能力的定量分析仍然缺乏，阻碍了对其功效的精确评估。本研究研制了一种含氧化钙（CaO2）的产氧胶原凝胶（oxy CG），并通过测量其产氧速率和计算其体积氧传递系数（kLa），定量分析了其氧释放动力学。利用这种方法，我们推导出了一个理论阈值的可培养细胞密度，并在缺氧条件下用原代大鼠肝细胞（PRHs）进行了实验验证。Oxy CG在维持pH稳定的同时持续释放氧气，支持肝细胞活力和肝脏特异性功能，如尿素合成和白蛋白分泌，在预测的细胞密度范围内。本研究提供了平衡氧气供应和细胞需求的定量框架，为优化肝组织工程中的氧气输送提供了有价值的见解，以克服3D构建中的扩散限制，提高临床适用性。

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引用次数: 0

13C-metabolic flux analysis of K562 cells before and after differentiation into erythroid reveals a metabolic shift toward oxidative metabolism K562细胞向红系分化前后的13c代谢通量分析显示其代谢向氧化代谢转变。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.jbiosc.2025.10.002

Eisuke Mochizuki , Nobuyuki Okahashi , Takeo Taniguchi , Fumio Matsuda

In regenerative medicine, it is crucial to discover the key factors associated with erythroid differentiation for efficient production of artificial red blood cells. One such factor is erythroid metabolism as erythroid cells dynamically coordinate their metabolic processes to obtain energy for proper differentiation. However, the details of these metabolic changes are not well understood. In this study, we aimed to analyze the metabolism of K562, a cell line that differentiates into erythroid cells using ¹³C-metabolic flux analysis. The results showed that differentiated cells decreased glycolytic flux and increased TCA cycle flux compared with undifferentiated cells, indicating a shift to oxidative metabolism via differentiation. Based on the finding, the inhibition of ATP synthase by oligomycin treatment significantly suppressed differentiation of K562 cells, suggesting that the activation of oxidative metabolism is required for proper differentiation of K562 cells.

在再生医学中，发现与红细胞分化相关的关键因素对于有效地生产人造红细胞至关重要。其中一个因素是红细胞代谢，红细胞动态协调其代谢过程以获得适当分化所需的能量。然而，这些代谢变化的细节尚不清楚。在这项研究中，我们旨在利用13c代谢通量分析K562这一分化为红系细胞的细胞系的代谢。结果表明，与未分化的细胞相比，分化的细胞糖酵解通量降低，TCA循环通量增加，表明细胞通过分化向氧化代谢转变。综上所述，寡霉素处理对ATP合酶的抑制显著抑制了K562细胞的分化，提示K562细胞的正常分化需要激活氧化代谢。

引用次数: 0

Evaluation of the skin whitening efficiency of Staphylococcus epidermidis fermentation broth and its oligopeptides 表皮葡萄球菌发酵液及其寡肽的皮肤美白效果评价。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-10-29 DOI: 10.1016/j.jbiosc.2025.10.001

Wenlin Geng, Ming Li, Yuhua Cao

In this work, two types of Staphylococcus epidermidis fermentation broth were prepared in beef-protein medium and beef-protein medium with glucose, named as SFB and Glu-SFB. As a positive control, 0.5 mg/mL kojic acid was utilized, which led to a 33.1 ± 1.32 % drop in melanin content and a 30.9 ± 2.95 % reduction in tyrosinase activity in B16–F10 cells. After treatment with SFB and Glu-SFB, the intracellular melanin content diminished by 35.4 ± 0.67 % and 48.5 ± 1.36 %, while tyrosinase activity declined by 59.1 ± 1.49 % and 64.4 ± 2.03 %, respectively. The two S. epidermidis fermentation broth markedly diminish intracellular melanin concentrations and tyrosinase activity, leading to a whitening effect. The whitening efficacy of Glu-SFB surpasses that of SFB and exceeds that of 0.5 mg/mL kojic acid. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with untargeted metabolomics analysis was utilized to identify differential metabolites. Six oligopeptides were identified as Trp-Phe-Tyr-Leu (WFYL), Gln-Ile-Gly-Pro (QIGP), Val-Arg-Phe-Ile (VRFI), Tyr-Ile-Arg (YIR), Glu-Gln-Ile-Trp (EQIW), and His-Gly-Tyr-Lys (HGYK), exhibiting greater relative abundance in Glu-SFB than in SFB. At a dosage of 0.1 mg/mL, oligopeptide exhibits a greater capacity to diminish intracellular melanin levels and tyrosinase activity compared to 0.5 mg/mL kojic acid. Gly-SFB is prepared by replacing glucose with glycerol, the relative concentration of oligopeptides in Gly-SFB is positioned between that of SFB and Glu-SFB, while its whitening efficacy is similarly intermediate between SFB and Glu-SFB. Western blot research shown that all the S. epidermidis fermentation broths may suppress the expression of tyrosinase (TYR), tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2), and microphthalmia-associated transcription factor (MITF), which is the primary mechanism underlying the whitening impact of these broths.

本研究在牛肉蛋白培养基和牛肉蛋白加葡萄糖培养基中制备了两种表皮葡萄球菌发酵液，分别命名为SFB和Glu-SFB。以0.5 mg/mL的曲酸作为阳性对照，B16-F10细胞黑色素含量下降33.1±1.32%，酪氨酸酶活性下降30.9±2.95%。经SFB和Glu-SFB处理后，细胞内黑色素含量分别下降35.4%±0.67%和48.5%±1.36%，酪氨酸酶活性分别下降59.1%±1.49%和64.4±2.03%。两种表皮葡萄球菌发酵液显著降低细胞内黑色素浓度和酪氨酸酶活性，从而达到美白效果。Glu-SFB的美白效果超过SFB，超过0.5 mg/mL的曲酸。采用液相色谱-串联质谱（LC-MS/MS）结合非靶向代谢组学分析鉴定差异代谢物。6个寡肽分别为：trp - fe - il - gly - pro （QIGP）、val - arg - fe - ile （VRFI）、tyr - il - arg （YIR）、glu - gln - il - trp （EQIW）和his - gln - il - lys (HGYK)，其中Glu-SFB中的相对丰度高于SFB。与0.5 mg/mL的曲酸相比，在0.1 mg/mL的剂量下，寡肽表现出更大的降低细胞内黑色素水平和酪氨酸酶活性的能力。Gly-SFB是用甘油代替葡萄糖制备的，Gly-SFB中寡肽的相对浓度介于SFB和Glu-SFB之间，其美白功效也同样介于SFB和Glu-SFB之间。Western blot研究表明，所有表皮葡萄球菌发酵液均可抑制酪氨酸酶（TYR）、酪氨酸酶相关蛋白-1 （TRP-1）、酪氨酸酶相关蛋白-2 （TRP-2）和小眼相关转录因子（MITF）的表达，这是这些发酵液美白作用的主要机制。

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引用次数: 0

Conversion of arachidonic acid into 14,15,19-trihydroxyeicosa-5,8,11-trienoic acid by Torula dematia NBRC 6213 花生四烯酸转化为14,15,19-三羟基二糖-5,8,11-三烯酸的研究。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-10-07 DOI: 10.1016/j.jbiosc.2025.09.004

Wataru Fujii , Michiki Takeuchi , Si-Bum Park , Kazuki Yagi , Shunta Nakamura , Ei-Tora Yamamura , Jun Ogawa

Lipids are one of the three major nutrients that play important roles as sources of energy and as components of cell membranes. Fatty acid metabolites exhibit physiological activities. Among fatty acid metabolites, some hydroxy arachidonic acids (ARAs) have bioactive functions. In this study, we found that Torula dematia NBRC 6213 converts ARA into unknown fatty acids. Liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry, and nuclear magnetic resonance (NMR) analyses were conducted to identify this unknown fatty acid. The unknown fatty acid was identified as 14,15,19-trihydroxyeicosa-5,8,11-trienoic acid. Its production was maximized when T. dematia was cultivated in potato dextrose broth (PDB) medium and reacted for 12 h at pH 7.0 and 28 °C. In addition, multiple intermediates were formed during the conversion of ARA to 14,15,19-trihydroxyeicosa-5,8,11-trienoic acid. LC-MS analysis revealed molecular weights of 320, 336, and 338. This suggests that ARA conversion occurs via the hydroxylation, epoxidation, and hydrolysis of the epoxy group. T. dematia also converts other unsaturated fatty acids into similarly oxidized fatty acids.

脂质是三种主要营养素之一，作为能量来源和细胞膜成分起着重要作用。脂肪酸代谢产物具有生理活性。在脂肪酸代谢产物中，一些羟基花生四烯酸具有生物活性。在这项研究中，我们发现Torula dematia NBRC 6213将ARA转化为未知的脂肪酸。采用液相色谱-质谱（LC-MS）、气相色谱-质谱（gc - ms）和核磁共振（NMR）分析对该未知脂肪酸进行鉴定。未知脂肪酸鉴定为14,15,19-三羟基二糖-5,8,11-三烯酸。在马铃薯葡萄糖肉汤（PDB）培养基中培养，在pH 7.0、28℃条件下反应12 h，其产量最大。此外，ARA在转化为14,15,19-三羟基二糖-5,8,11-三烯酸的过程中形成了多个中间体。LC-MS分析显示分子量为320、336和338。这表明ARA转化通过羟基化、环氧化和环氧基水解发生。脱脂腺也将其他不饱和脂肪酸转化为类似的氧化脂肪酸。

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引用次数: 0

Aspergillus oryzae strains heterogeneously produce plant polysaccharide degradation-related enzymes 米曲霉菌株异质产生植物多糖降解相关酶。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-11-10 DOI: 10.1016/j.jbiosc.2025.10.008

Shimma Fujiwa , Ryousuke Kataoka , Kazuhiro Iwashita , Tomohiko Matsuzawa

Aspergillus oryzae is used for brewing, and many strains with different brewing characteristics have been isolated. We compared enzymatic activities of eight A. oryzae strains, RIB40, 128, 143, 163, 301, 915, 1108, and 1178 strains, toward plant polysaccharides. The plant polysaccharide degradation-related enzymes produced by A. oryzae change depending on the monosaccharides and polysaccharides added to the culture medium. RIB915 produced more cellulolytic and hemicellulolytic enzymes, whereas RIB40, 128, and 301 produced less of these enzymes than the other strains. In addition, A. oryzae RIB128 produced different glycoside hydrolases in response to monosaccharides and polysaccharides compared with other strains. These results indicated that there is diversity in the production of plant polysaccharide degradation-related enzymes within A. oryzae species.

米曲霉用于酿造，已分离出许多具有不同酿造特性的菌株。我们比较了8株稻瘟病菌RIB40、128、143、163、301、915、1108和1178菌株对植物多糖的酶活性。m.o ryzae产生的植物多糖降解相关酶随着培养基中添加的单糖和多糖的不同而变化。与其他菌株相比，RIB915产生更多的纤维素分解酶和半纤维素分解酶，而RIB40、128和301产生的这些酶较少。此外，与其他菌株相比，A. oryzae RIB128对单糖和多糖产生不同的糖苷水解酶。这些结果表明，米芽孢杆菌在植物多糖降解相关酶的生产中存在多样性。

引用次数: 0

Characterization of a molting-related chitinase from a land crab, Chiromantes haematocheir 一种陆地蟹（Chiromantes haematocheir）几丁质酶的特性。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-10-07 DOI: 10.1016/j.jbiosc.2025.09.006

Yuma Nagakura, Katsuhide Miyake

Chitinases play an important role in many biological processes, including molting, digestion, and immunity in crustaceans. This study represents an attempt to apply chitinases in the field of biotechnology, detecting chitinase mRNAs from a land crab, Chiromantes haematocheir, via transcriptome analysis and analyzing their properties. Seven chitinase genes were detected from the RNA-seq data of midgut glands. Among these genes, TRINITY_DN29294 transcripts accounted for virtually all of total expression of the chitinases. The 29294 cDNA contained a 1467 bp open reading frame, coded for 488 amino acid residues, and was classified into the GH18 chitolectin chitotriosidase and the group 3 crab chitinase. The expression of the 29294-chitinase mRNA was detected in all tissues, with the highest levels expressed in the midgut glands. The transcripts increased significantly in the early post-molted crab compared to the non-molting crab. These results suggest that 29294-chitinase plays important roles in the molting process. While the recombinant 29294-chitinase over-produced in Escherichia coli did not show any activity, the enzyme expressed in Pichia pastoris exhibited sufficient activity. The 29294-chitinase had its optimal pH 3.0. The optimal temperature was relatively high at 45 °C. The enzyme hydrolyzed both soluble and crystalline substrates.

几丁质酶在甲壳类动物的蜕皮、消化和免疫等生物过程中起着重要作用。本研究尝试将几丁质酶应用于生物技术领域，通过转录组分析检测陆地蟹Chiromantes haematocheir的几丁质酶mrna并分析其性质。从中肠腺的RNA-seq数据中检测到7个几丁质酶基因。在这些基因中，TRINITY_DN29294转录本几乎占几丁质酶总表达量的全部。29294 cDNA包含1467 bp的开放阅读框，编码488个氨基酸残基，被分类为GH18壳聚糖壳三醇苷酶和第3组螃蟹几丁质酶。29294-几丁质酶mRNA在各组织中均有表达，其中以中肠腺表达量最高。与未蜕皮的蟹相比，早期蜕皮蟹的转录本显著增加。上述结果表明，29294-几丁质酶在玉米的蜕皮过程中起着重要作用。在大肠杆菌中过量产生的重组29294-几丁质酶没有表现出任何活性，而在毕赤酵母中表达的酶表现出足够的活性。29294-几丁质酶的最佳pH值为3.0。最佳温度为45℃。这种酶既能水解可溶性底物，也能水解结晶底物。

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引用次数: 0

Lipid metabolism prediction in oleaginous yeasts across taxonomic levels using single-cell innate fluorescence signature 利用单细胞先天荧光标记预测产油酵母在不同分类水平上的脂质代谢。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-10-31 DOI: 10.1016/j.jbiosc.2025.10.003

Shiomi Yawata , Tomohiro Hirayama , Tatsuro Serita , Haruka Kazama , Koji Mori , Hiroaki Takaku , Nobuhiko Nomura , Yutaka Yawata

Cellular innate fluorescence (IF) is a natural fluorescence derived from cellular metabolites and biomolecules within microbial cells. Although IF is suggested to be a promising tool for probing the physiology of cells in a non-invasive manner, the link between single-cell IF and heterogeneity in material production remains largely unexplored. This study aimed to examine the link between cellular IF in oleaginous yeasts and their lipid-producing capabilities at multiple taxonomic levels: intra-species, inter-species, and inter-genus. Briefly, we developed a novel microscopic method that can directly link cellular IF (a single-cell IF signature) and the lipid-producing capability of cells at single-cell resolution, thereby enabling the recognition of high heterogeneity in single-cell IF and lipid production in cells. At the intra-species level, the time-course analysis revealed a parallelism between the shifts in single-cell IF signatures and lipid production by Lipomyces starkeyi. The regression model constructed based on single-cell IF signatures could predict intracellular lipid contents. The link between IF and lipid production was also observed across the inter-strain, inter-species, and inter-genus levels, where the regression model constructed with single-cell IF signatures of different strains, species, and genera could predict lipid production. Machine learning models established a computational link to predict lipid productivity by relying on the single-cell IF signatures. These results indicate that the single-cell IF signature is a promising tool for lipid production analysis and prediction in oleaginous yeast species across taxa.

细胞固有荧光（IF）是微生物细胞内代谢产物和生物分子产生的一种天然荧光。虽然中频被认为是一种很有前途的工具，可以以非侵入性的方式探测细胞的生理机能，但单细胞中频与物质生产异质性之间的联系在很大程度上仍未被探索。本研究旨在从种内、种间和属间等多个分类水平上研究产油酵母细胞IF与其产脂能力之间的联系。简而言之，我们开发了一种新的显微镜方法，可以直接将细胞中频（单细胞中频特征）与细胞在单细胞分辨率下的脂质产生能力联系起来，从而能够识别单细胞中频和细胞中脂质产生的高度异质性。在种内水平上，时间过程分析揭示了单细胞IF特征的变化与starkeyi脂质产生之间的平行性。基于单细胞IF特征构建的回归模型可以预测细胞内脂质含量。在菌株间、种间和属间水平上也观察到IF与脂质产生之间的联系，其中利用不同菌株、种和属的单细胞IF特征构建的回归模型可以预测脂质产生。机器学习模型建立了一个计算链接，通过依赖单细胞IF特征来预测脂质生产力。这些结果表明，单细胞IF标记是一种很有前途的工具，可以用于分析和预测产油酵母在不同分类群中的产脂过程。

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引用次数: 0

Production of 1-(β-d-ribofuranosyl)-1,2,4-triazole through solid-state fermentation with Purpureocillium lavendulum DQWM-G4 紫紫色紫霉DQWM-G4固态发酵生产1-（β-d-核呋喃基）- 1,2,4-三唑

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2026-01-01 Epub Date: 2025-11-01 DOI: 10.1016/j.jbiosc.2025.10.005

Jian-Wei Dong, Meng Liu, Xue-Jiao Li, Ke-Wen Pan

1-(β-d-Ribofuranosyl)-1,2,4-triazole (RTA) is a nucleotide analog of 1,2,4-triazole that demonstrates broad-spectrum antiviral activity. It is currently the approved drug for treating chronic hepatitis E virus (HEV) infections. However, the production of RTA relies predominantly on chemical synthesis and extraction from plants and animals, with no report of microbial metabolic production. This study introduces a novel approach to producing RTA via solid-state fermentation (SSF) using Purpureocillium lavendulum DQWM-G4, with rice as the substrate. The chemical structure of RTA was elucidated through ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy. Fermentation conditions for producing RTA were optimized by investigating substrate type, temperature, and duration. Under optimized conditions, SSF with P. lavendulum DQWM-G4 on rice at 20 °C for 60 d yielded RTA at a high concentration of 3.46 ± 0.16 mg/g. This paper represents the first report on microbial production of RTA, offering an alternative to chemical synthesis and natural extraction.

1-（β-d-核糖呋喃基）-1,2,4-三唑（RTA）是1,2,4-三唑的核苷酸类似物，具有广谱抗病毒活性。它是目前批准用于治疗慢性戊型肝炎病毒（HEV）感染的药物。然而，RTA的生产主要依赖于化学合成和从植物和动物中提取，没有微生物代谢生产的报道。本研究介绍了一种以水稻为底物，利用紫紫色毛霉DQWM-G4固态发酵生产RTA的新方法。通过1H和13C核磁共振（NMR）谱分析了RTA的化学结构。通过考察底物类型、发酵温度和发酵时间，优化了RTA的发酵条件。在优化条件下，黄花苜蓿DQWM-G4的SSF在水稻上20℃作用60 d， RTA浓度为3.46±0.16 mg/g。本文首次报道了微生物生产RTA，为化学合成和天然提取提供了一种替代方法。

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引用次数: 0

Whole-genome draft assemblies of Paracoccus pantotrophus DSM 11073 and Paracoccus sp. AS002: Phylogenetics entails classification as Paracoccus versutus AS002 泛养副球菌DSM 11073和副球菌sp. AS002的全基因组草图组装：系统发育需要分类为反副球菌AS002。

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering

Pub Date : 2025-12-01 Epub Date: 2025-09-27 DOI: 10.1016/j.jbiosc.2025.09.003

Upasana Pal , Denise Bachmann , Linda Fenske , Lars Mathias Blank , Till Tiso

The Gram-stain-negative bacterium Paracoccus spp., from the class Alphaproteobacteria and family Rhodobacteraceae, exhibits exceptional metabolic flexibility, diverse substrate utilization, and tolerance to abiotic stressors. To broaden the biotechnological applications of the genus, comprehensive sequencing, phylogenetic, and physiological characterization were performed between two strains of the genus, Paracoccus pantotrophus DSM 11073 and Paracoccus sp. AS002. Illumina sequencing yielded a total genome size of 4.2 Mbp for P. pantotrophus DSM 11073 and 4.8 Mbp for Paracoccus sp. AS002. Through phylogenetic analysis using the EDGAR software, Paracoccus sp. AS002 shared with Paracoccus versutus DSM 582 the same clade in the phylogenetic tree and an ANI score of 98.9 % indicating that Paracoccus sp. AS002 could be reclassified as P. versutus AS002. The study was extended to compare various attributes of the sequenced genomes and highlight the metabolic versatility of the genus Paracoccus. The use of a wide substrate panel demonstrated the metabolic versatility of the strains, including the PET monomer ethylene glycol, the C1 carbon source formic acid, and renewable carbon sources such as ethanol. Additionally, the ability of the strains to produce bioplastic was assessed, with P. pantotrophus DSM 11073 producing 36 % and P. versutus AS002 28 % polyhydroxybutyrate (% cell dry weight) on glucose, and 40 % and 16 % on 60 mM ethylene glycol, respectively. This study demonstrates the value of sequencing bacterial strains for biotechnological applications and highlights EDGAR's effectiveness in phylogenetic analysis, paving the way for using Paracoccus as a microbial chassis in sustainable biotechnological processes supporting the circular bioeconomy.

革兰氏染色阴性的副球菌属，属于α变形菌纲和红杆菌科，表现出特殊的代谢灵活性，多种底物利用和对非生物应激源的耐受性。为了扩大该属的生物技术应用，对两株pantotrophus Paracoccus DSM 11073和Paracoccus sp. AS002进行了全面的测序、系统发育和生理特性研究。Illumina测序结果显示，P. pantotrophus DSM 11073的总基因组大小为4.2 Mbp，副球菌sp. AS002的总基因组大小为4.8 Mbp。通过EDGAR软件进行系统发育分析，发现副球菌sp. AS002与反苏副球菌DSM 582在系统发育树上具有相同的进化支，ANI评分为98.9%，表明副球菌sp. AS002可以重新归类为反苏副球菌AS002。该研究扩展到比较测序基因组的各种属性，并突出副球菌属的代谢多样性。广泛底物面板的使用证明了菌株代谢的多功能性，包括PET单体乙二醇、C1碳源甲酸和可再生碳源如乙醇。此外，对菌株产生生物塑料的能力进行了评估，P. pantotrophus DSM 11073在葡萄糖上产生36%的聚羟基丁酸盐，P. versutus AS002在60 mM乙二醇上产生28%的聚羟基丁酸盐（细胞干重的%），分别为40%和16%。该研究证明了细菌菌株测序在生物技术应用中的价值，并强调了EDGAR在系统发育分析中的有效性，为利用副球菌作为支持循环生物经济的可持续生物技术过程中的微生物基础铺平了道路。

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引用次数: 0