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Nutritive Value and Health Benefit of Fermented Milks 发酵乳的营养价值和健康益处
Pub Date : 2018-05-21 DOI: 10.37591/RRJODST.V2I3.859
Tanmay Hazra, Kamal Gandhi, Anamika Das
Fermented milk plays a very vital role in human civilization from ancient time. Fermented milk product is not only nutritionally superior but it has plenty of health benefits, though all the health benefit mechanisms of fermented milk is not known but in future fermented milk would be one of the substitute of medicine. Keywords: Lactic acid, β-galactosidase, biological value, anticholesterolemic
自古以来,发酵乳在人类文明中起着至关重要的作用。发酵乳不仅营养价值优越,而且对健康有益,虽然发酵乳对健康的益处机制尚不清楚,但在未来发酵乳将成为药物的替代品之一。关键词:乳酸,β-半乳糖苷酶,生物学价值,抗胆固醇
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引用次数: 2
Maillard Browning: Pros and Cons in Dairy and Food Industries 美拉德·布朗宁:乳制品和食品工业的利弊
Pub Date : 2018-05-21 DOI: 10.37591/RRJODST.V2I2.853
H. Kumar, N. Choudhary, Varsha Garg, N. Swami, H. Kumar, R. Seth
The Maillard reaction, also known as non-enzymatic browning reaction between reducing sugars and amino groups. This reaction is responsible for the formation of several compounds, called MRP (Maillard Reaction Products) for food products. The Maillard reaction produces undesirable effects during the processing and storage of different liquid foods such as milk or fruit juices whereas for other solid foods the changes are favorable (in the case of bread, breakfast cereals, candies, coffee, chocolate, etc.). The Maillard reaction is a complex reaction, since it is influenced by many factors such as temperature, pH, time, water activity, type and concentration of reactant source and sugar involved. In heated food products, two carcinogenic compounds are produced during maillard reactions, acrylamide and imidazoquinoline. Furosine and HMF are two compounds that indicate the extent of the maillard reaction related to the type and intensity of the food processing conditions. Keywords: Maillard reaction, melanoidin, furfural, amadori rearrangement
美拉德反应,也称为还原糖和氨基之间的非酶褐变反应。这一反应导致了几种化合物的形成,这些化合物在食品中被称为MRP(美拉德反应产物)。美拉德反应在不同液体食品(如牛奶或果汁)的加工和储存过程中产生不良影响,而对其他固体食品(如面包、早餐谷物、糖果、咖啡、巧克力等)的变化是有利的。美拉德反应是一个复杂的反应,它受温度、pH、时间、水活度、反应物来源的类型和浓度以及所涉及的糖等多种因素的影响。在加热的食品中,在美拉德反应中会产生两种致癌化合物:丙烯酰胺和咪唑喹啉。糠氨酸和HMF是指示美拉德反应程度的两种化合物,与食品加工条件的类型和强度有关。关键词:美拉德反应,类黑素,糠醛,amadori重排
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引用次数: 4
Effect of Vidarikand (Extracts) on Oxidative Stability of Ghee: A Comparative Study 维他兰(提取物)对酥油氧化稳定性影响的比较研究
Pub Date : 2018-05-21 DOI: 10.37591/RRJODST.V2I1.847
Kamal Gandhi, S. Arora, Nilkanth Pawar, Anil Kumar
Antioxidant activities of vidarikand (Pueraria tuberosa) extracts were evaluated and compared with BHA, TBHQ, rosemary and green tea using a β-carotene bleaching assay, a 2, 2-diphenyl-β-picrylhydrazyl (DPPH) assay and the Rancimat method. Phenolic content and antioxidant activity (β-carotene–linoleic acid model system and DPPH assay) of ethanolic extract of vidarikand was more compared to its aqueous extract. Ethanolic extract of the vidarikand was more effective in preventing the development of the peroxide value and conjugated diene value in ghee compared to its aqueous extract. Vidarikand ethanolic extract showed the higher induction period as compared to its aqueous extract in the Rancimat. Keywords: antioxidant activity, ghee (butter oil), vidarikand (Pueraria tuberosa), phenolic content, radical-scavenging activity, rancimat, antioxidant activity
采用β-胡萝卜素漂白法、2,2 -二苯基-β-苦酰肼(DPPH)法和rangimat法评价了维达理兰(葛根)提取物的抗氧化活性,并与BHA、TBHQ、迷迭香和绿茶进行了比较。维他兰醇提物的酚含量和抗氧化活性(β-胡萝卜素-亚油酸模型体系和DPPH测定)均高于水提物。与水萃取物相比,维达利兰乙醇萃取物在防止酥油中过氧化值和共轭二烯值的发展方面更有效。维达利兰乙醇提取物的诱导期高于水提取物。关键词:抗氧化活性,酥油,维达力兰,酚含量,自由基清除活性,香料,抗氧化活性
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引用次数: 21
Isolation and Characterization of Lactobacillus spp. of Human Origin for Studying their Prevalence 人源乳杆菌的分离鉴定及其流行病学研究
Pub Date : 2018-05-21 DOI: 10.37591/RRJODST.V2I3.857
M. Puniya, K. Sangu, A. Bhardwaj, Sanjay Kumar, T. Dhewa
A total of 15 samples of healthy human feces were collected for the isolation of lactobacilli using lactobacillus selection MRS agar. Thirty-eight colonies were randomly picked based on colonial morphology. All the isolates were subjected to cell morphology, physiology and an array of biochemical characterization. The isolates showed different growth patterns at different temperatures (15 and 45 °C), oxygen and at different concentrations of NaCl (2.0, 4.0 and 6.5%). On the basis of physiological tests and sugar utilization pattern, all the thirty eight isolates were confirmed to the different species of Lactobacillus (i.e., Lactobacillus plantarum (4), Lactobacillus casei (15), Lactobacillus rhamnosus (2), Lactobacillus fermentum (4), and Lactobacillus acidophilus (10), Lactobacillus helveticus (3). Among isolates Lactobacillus casei was found to be prevalent in the human gut. Keywords: Lactobacilli, biochemical characterization, human gut
收集15例健康人粪便标本,采用乳酸菌筛选MRS琼脂进行乳酸菌分离。根据群落形态随机选取38个群落。所有分离株都进行了细胞形态学、生理学和一系列生化表征。菌株在不同温度(15°C和45°C)、氧气和不同NaCl浓度(2.0、4.0和6.5%)下表现出不同的生长模式。根据生理试验和糖利用模式,38株分离菌均为不同种类的乳杆菌,分别为植物乳杆菌(4株)、干酪乳杆菌(15株)、鼠李糖乳杆菌(2株)、发酵乳杆菌(4株)、嗜酸乳杆菌(10株)、helveticus乳杆菌(3株)。其中,干酪乳杆菌在人体肠道中普遍存在。关键词:乳酸菌,生化表征,人肠道
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引用次数: 1
Effect of Prebiotics on Growth of the Selected Lactobacilli Culture Isolated from Dairy Products 益生元对乳制品乳酸菌生长的影响
Pub Date : 2018-05-21 DOI: 10.37591/RRJODST.V2I2.852
A. Bhardwaj, M. Puniya, K. Sangu, Sanjay Kumar, T. Dhewa
Prebiotics beneficially affects the host by selectively stimulating the growth or activity of one or limited number of bacteria such as bifidobacteria, lactobacilli in the colon that can improve the host health. Inulin and gum acacia are the most commonly available prebiotics. The ability of lactobacilli cultures (i.e. Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei) to utilize inulin, gum acacia, honey and fructo-oligosaccharide @ 2% for different time intervals (6, 12 and 24 h) were tested. The growth rate of Lactobacillus acidophilus was found be higher in 2% honey than other isolates as well as prebiotics taken. Potential probiotic Lactobacillus acidophilus isolated from curd were allowed to grow in selected prebiotics, i.e., honey at different concentrations (2.0, 3.0 and 4.0%) for a time period of 0–24 h. Lactobacillus acidophilus, could utilize all the concentrations of honey (2.0, 3.0, and 4.0%) with control (glucose, 1%) and exhibited maximum growth 4.5 x 107 at 6 h interval, 5.4 x 108 at 12 h time period and 6.3x109 after 24 h of incubation. At 2% honey concentration the growth rate is nearly similar to that of 3% honey. Whereas, at 4% honey the growth of probiotic bacteria decrease in comparison to that of 2 and 3%. When combining both lactobacilli cultures (potential probiotics) and a prebiotic in a single food product, the expected benefits are an improved survival during the passage of probiotic bacteria through the upper intestinal tract. Keywords: prebiotics, lactobacilli, host health
益生元通过选择性地刺激一种或有限数量的细菌(如双歧杆菌、乳酸杆菌)的生长或活性,对宿主产生有益的影响,从而改善宿主的健康。菊粉和金合欢胶是最常见的益生元。测试了乳酸菌培养物(即嗜酸乳杆菌、植物乳杆菌和干酪乳杆菌)在不同时间间隔(6、12和24 h)对2%菊粉、金合木、蜂蜜和低聚果糖的利用能力。在2%蜂蜜中发现嗜酸乳杆菌的生长速度高于其他分离株和益生元。从凝乳中分离出的潜在益生菌嗜酸乳杆菌可以在选定的益生元中生长,即在不同浓度(2.0、3.0和4.0%)的蜂蜜中生长0-24 h。嗜酸乳杆菌可以利用所有浓度的蜂蜜(2.0、3.0和4.0%)和对照(葡萄糖,1%),孵育间隔6 h时最大生长4.5 × 107, 12 h时最大生长5.4 × 108,孵育24 h后最大生长6.3 × 109。在蜂蜜浓度为2%时,其生长速率与蜂蜜浓度为3%时几乎相同。然而,与蜂蜜浓度为2%和3%相比,蜂蜜浓度为4%时益生菌的生长减少。当将乳酸菌培养物(潜在的益生菌)和益生元结合在单一食品中时,预期的好处是益生菌通过上肠道时存活率的提高。关键词:益生元,乳酸菌,宿主健康
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引用次数: 2
Assessment of In Vitro Probiotic Potential of Lactic Acid Bacteria 乳酸菌体外益生菌潜力的评价
Pub Date : 2018-04-21 DOI: 10.37591/RRJODST.V5I1.487
Mital R. Kathiriya, S. Hati, J. Prajapati, Y. Vekariya
Abstract Probiotic potential of fermented milks isolates Lactobacillus rhamnosus NS6, Streptococcus thermophilus MD2 and Streptococcus thermophilus MD8 were carried out by performing various in vitro tests. MD2 and MD8 were able to survive at pH 2 and 3 in broth, while NS6 was found to be pH sensitive and could not survive at pH 2, but maintained its viability at 3 pH. All the cultures were able to survive at 0.5 % (w/v) oxgall (bile) concentration in broth. NS6 was most resistant to bile than rest isolates. They were susceptible to ampicilin, azithromycin, tetracycline, gentamycin and erythromycin while these strains were resistant to nalidixic acid, oxacilin, colistin and kanamycin. Cell supernatant of NS6 showed a higher antimicrobial activity i.e., 24 mm zone against E. coli and S. aureus; 16 mm zone against B. cereus and S. typhi and neutralization of cell supernatant showed significant reduction in antimicrobial activity. All the three strains were hydrophobic to both, xylene and n-hexadecan. The mean percentage hydrophobicity was higher to xylene than n-hexadecanfor all the strains. Cell-auto-aggregation was found to be rising during 5 h of incubation for cultures. They were able to co-aggregate with B. cereus, S, typhi, E. coli and S. aureus (indicators). None of the culture could hydrolyze sodium taurocholate (bile) while they deconjugated sodium taurocholate to release free cholic acid. NS6 gave maximum bile deconjugation ability (364 µg/ml). Similarly, in case of cholesterol reduction and antioxidative activity (ABTS method), NS6 was more potent than others. Keywords: Lactobacillus rhamnosus, Streptococcus thermophilus, lactic acid bacteria, probiotics, bile Cite this Article M.R. Kathiriya, S. Hati, J.B. Prajapati et al. Assessment of In Vitro Probiotic Potential of Lactic Acid Bacteria. Research & Reviews: Journal of Dairy Science and Technology. 2016: 5(1):  17–30p.
摘要通过各种体外实验,研究了发酵乳分离物鼠李糖乳杆菌NS6、嗜热链球菌MD2和嗜热链球菌MD8的益生菌潜力。MD2和MD8在pH为2和3的培养液中均能存活,而NS6对pH敏感,在pH为2的培养液中不能存活,但在pH为3的培养液中仍能存活。所有培养物在0.5% (w/v)的氧化胆(胆汁)培养液中均能存活。NS6对胆汁的耐药程度高于其余分离株。对氨苄西林、阿奇霉素、四环素、庆大霉素和红霉素敏感,对萘啶酸、恶西林、粘菌素和卡那霉素耐药。NS6细胞上清液对大肠杆菌和金黄色葡萄球菌具有较高的抑菌活性(24 mm区);16 mm区对蜡样芽孢杆菌和斑疹伤寒链球菌的抑菌活性显著降低。3株菌株对二甲苯和正十六烷均有疏水性。所有菌株对二甲苯的平均疏水性百分比均高于正十六烷。在培养5小时的培养过程中发现细胞自身聚集增加。它们能够与蜡样芽孢杆菌、S、伤寒杆菌、大肠杆菌和金黄色葡萄球菌(指标)共聚集。没有一种培养物能水解牛磺酸胆酸钠(胆汁),但它们能解牛磺酸胆酸钠释放游离胆酸。NS6具有最大的胆汁解结能力(364µg/ml)。同样,在降低胆固醇和抗氧化活性(ABTS法)的情况下,NS6比其他药物更有效。关键词:鼠李糖乳杆菌,嗜热链球菌,乳酸菌,益生菌,胆汁乳酸菌体外益生菌潜力的评价。研究综述:乳品科技,2016,5(1):17-30p。
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引用次数: 6
Influence of Genetic and Non-Genetic Factors on Lactation Traits in Dairy Cattle: A Review 遗传和非遗传因素对奶牛泌乳性状的影响
Pub Date : 2018-04-21 DOI: 10.37591/rrjodst.v5i3.452
P. Ratwan, Manoj Kumar, A. Mandal
India has about 39.73 million crossbred cattle out of total 190.90 million (37.28%) cattle population as per the all India livestock census, 2012. The decade wise trend in livestock population (1997 to 2012) shows a distinctive swing in composition of dairy animal stock in favor of crossbred cattle, as their number increased by 20.18%, while that of indigenous cattle declined by 8.94%. Selection of dairy animals is usually based on the lactation traits. All the lactation traits are affected by various genetic and non-genetic factors like sire, season of calving, period of calving, parity of animals, age group and genetic group of animals. Accurate estimation or unbiased prediction of genetic parameters depends upon the adjustment of significant effect of environmental or non-genetic factors on lactation traits.  The lactation traits reviewed were 305 days milk yield (305MY), total lactation milk yield (TMY), lactation length (LL), peak yield (PY) and milk production efficiency traits comprised of milk yield per day of lactation length (MY/LL) and milk yield per day of calving interval (MY/CI). In order to enhance the productivity, it is essential to develop an understanding of the factors affecting lactation traits in dairy cattle. Keywords: Genetic and non-genetic factors, dairy cattle, lactation traits Cite this Article Poonam Ratwan, Manoj Kumar, Ajoy Mandal. Influence of Genetic and Non-Genetic Factors on Lactation Traits in Dairy Cattle: A Review. Research & Reviews: Journal of Dairy Science and Technology . 2016; 5(3): 7–22p.
根据2012年全印度牲畜普查,印度共有19909万头牛(37.28%),其中杂交牛约有3973万头。牲畜种群的十年趋势(1997年至2012年)表明,奶牛种群的组成明显倾向于杂交牛,杂交牛的数量增加了20.18%,而土生牛的数量减少了8.94%。奶牛的选择通常以泌乳性状为基础。所有的泌乳性状都受到各种遗传和非遗传因素的影响,如母猪的父系、产犊季节、产犊期、胎次、动物的年龄和遗传群等。遗传参数的准确估计或无偏预测取决于环境或非遗传因素对泌乳性状的显著影响的调整。研究的泌乳性状包括305日产奶量(305MY)、总泌乳量(TMY)、泌乳长度(LL)、峰值产奶量(PY)和产乳效率性状,包括泌乳长度/日产奶量(MY/LL)和产犊间隔/日产奶量(MY/CI)。为了提高奶牛的生产能力,有必要了解影响奶牛泌乳性状的因素。关键词:遗传与非遗传因素;奶牛;泌乳性状遗传和非遗传因素对奶牛泌乳性状的影响研究与评述:乳品科学与技术杂志。2016;5 (3): 7-22p。
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引用次数: 6
Significance of Butyrophilin Gene in Relation to Milk Constituents in Dairy Animals 亲丁酸基因对乳动物乳成分的影响
Pub Date : 2018-04-21 DOI: 10.37591/rrjodst.v6i1.531
P. Ratwan, Manoj Kumar, V. Vohra
Butyrophilin (BTN1A1) has been categorized as member of transmembrane proteins of immunoglobulin family. BTN1A1 is a QTL candidate gene having role in milk production and composition (fat) in dairy animals. The gene product BTN1A1 has function during secretion of milk fat and its expression is tissue specific in lactating mammary tissue. BTN1A1 constitutes more than 40% by weight of total protein associated with fat globule membrane of bovine milk. BTN1A1 is synthesized as a peptide of 526 amino acids with an amino-terminal hydrophobic signal sequence of 26 amino acids which is cleaved before secretion in association with milk fat globule membrane (MFGM). Cholesterolemia-lowering factor, inhibitors of cancer cell growth, vitamin binders and inhibitor of various bacterial diseases are different health favorable components of MFGM. The gene frequency of polymorphism in BTN1A1 gene has been found to vary among dairy animals. In dairy animals, several studies revealed association of BTN1A1 gene with high milk yield, fat content and protein yield. These associations suggest gene polymorphisms that can be further utilized for selecting superior dairy animals. Keywords: bovine , butyrophilin, milk fat globule membrane (MFGM), milk fat secretion Cite this Article Ratwan P, Kumar M, Vohra V. Significance of Butyrophilin Gene in Relation to Milk Constituents in Dairy Animals. Research & Reviews: Journal of Dairy Science and Technology . 2017; 6(1): 24–30p.
Butyrophilin (BTN1A1)是免疫球蛋白家族跨膜蛋白中的一员。BTN1A1是一个与奶牛产奶量和脂肪组成有关的QTL候选基因。基因产物BTN1A1在乳脂分泌过程中发挥作用,其表达在泌乳乳腺组织中具有组织特异性。BTN1A1占牛乳脂肪球膜相关蛋白总重量的40%以上。BTN1A1是一个由526个氨基酸组成的肽,其氨基端有26个氨基酸的疏水信号序列,在分泌前与乳脂球膜(MFGM)相关。降胆固醇因子、癌细胞生长抑制剂、维生素结合剂和各种细菌疾病抑制剂是MFGM的不同健康有益成分。BTN1A1基因多态性的基因频率在奶牛中存在差异。在奶牛中,一些研究发现BTN1A1基因与高产奶量、脂肪含量和蛋白质产量有关。这些关联表明基因多态性可以进一步用于选择优质奶牛。关键词:牛,亲丁蛋白,乳脂球膜(MFGM),乳脂分泌研究与评述:乳品科学与技术杂志。2017;6 (1): 24-30p。
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引用次数: 2
Heterosis–Genetic Basis and Utilization for Improvement of Dairy Cattle: A Review 奶牛杂种优势遗传基础及其改良利用综述
Pub Date : 2018-04-21 DOI: 10.37591/RRJODST.V5I2.479
Manoj Kumar, P. Ratwan, Anil Kumar
The phenomenon in which progeny of crosses between inbred lines or purebred populations are better than the expected average of the two populations or lines for a particular trait is known as heterosis. Utilization of heterosis is the main goal of crossbreeding. The amount of heterosis maintained in a herd depends on the type of crossbreeding system selected for breeding. Heterosis includes greater viability, faster growth rate and greater milk production in dairy cattle. The genetic basis of heterosis is nonadditive gene action (dominance, overdominance, pseudo-overdominance, and epistasis). The main requirements for heterosis are there must be genetic diversity between the breeds crossed and there must be some nonadditive gene effects present for the particular trait involved. No heterosis was observed for traits governed by additive gene action. However, it tends to be greatest for traits with low heritability and least for traits with high heritability in nature. Traits of low heritability (reproductive traits) are generally most benefited from heterosis as compare to high heritability (growth rate). They can be improved through the adequate use of crossbreeding systems. Keywords: Crossbreeding, heterosis, cattle Cite this Article Kumar M, Ratwan P,  Kumar A. Heterosis–Genetic Basis and Utilization for Improvement of Dairy Cattle: A Review. Research & Reviews: Journal of Dairy Science and Technology . 2016; 5(2): 40–50p.
自交系或纯种群体之间的杂交后代在某一特定性状上优于两个群体或系的预期平均值的现象称为杂种优势。杂种优势的利用是杂交育种的主要目的。在一个畜群中保持杂种优势的数量取决于选择杂交育种系统的类型。杂种优势包括奶牛更强的生存能力、更快的生长速度和更高的产奶量。杂种优势的遗传基础是非加性基因作用(显性、超显性、伪超显性和上位性)。杂种优势的主要要求是杂交品种之间必须具有遗传多样性,并且在所涉及的特定性状上必须存在一些非加性基因效应。加性基因作用下的性状未见杂种优势。但在自然界中,遗传力低的性状对遗传力的影响最大,遗传力高的性状对遗传力的影响最小。与高遗传力(生长率)相比,低遗传力性状(生殖性状)通常从杂种优势中获益最多。它们可以通过充分利用杂交育种系统得到改善。库马尔·M,拉特万·P,库马尔·A。奶牛杂种优势的遗传基础及其改良利用综述。研究与评述:乳品科学与技术杂志。2016;5 (2): 40-50p。
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引用次数: 2
Status and Market Potential of Oats-Based Fermented Milk Products 燕麦发酵乳制品的现状与市场潜力
Pub Date : 2018-04-21 DOI: 10.37591/RRJODST.V6I2.498
K. Gawai, S. Balakrishanan
Fermented milk is considered best career of probiotics with its nutritive composition and natural buffering capacity. Positive health effects of fermented foods and especially of those with probiotic microorganisms are reported in many recent publications. Most common food matrices used to deliver probiotic are dairy-based products; plant-based foods, dried formulations and blended products. In connection with these health effects of probiotics ingredients such as WPC, WPI, and Na-Cn improve nutritional values and biological effects of yogurt on health. It could be interpreted that nutritional components from milk, oat (Avena sativa L.) and probiotic bacteria could be a novel concept of designing a functional food. Limited reports are available with regards to the development of fermented product using oat components and milk to produce yoghurt-like products. Keywords: Oats, fermented milks, market, health benefits, β-glucan Cite this Article Gawai KM, Balakrishanan S. Status and Market Potential of Oats-Based Fermented Milk Products. Research & Reviews: Journal of Dairy Science and Technology . 2017; 6(2): 1–6p.
发酵乳以其营养成分和天然缓冲能力被认为是益生菌的最佳来源。最近的许多出版物都报道了发酵食品,特别是那些含有益生菌微生物的发酵食品对健康的积极影响。用于输送益生菌的最常见食物基质是乳制品;植物性食品,干燥配方和混合产品。益生菌成分如WPC、WPI和Na-Cn提高了酸奶的营养价值和对健康的生物效应。从牛奶、燕麦和益生菌中提取营养成分可能是设计功能性食品的新概念。关于使用燕麦成分和牛奶生产酸奶类产品的发酵产品的开发,报告有限。关键词:燕麦,发酵乳,市场,健康益处,β-葡聚糖引用本文Gawai KM, Balakrishanan S.燕麦发酵乳的现状和市场潜力。研究与评述:乳品科学与技术杂志。2017;6 (2): 1-6p。
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引用次数: 0
期刊
Journal of Dairy Science and Technology
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