Background. Enamel microabrasion can eliminate enamel irregularities and discoloration. This study evaluated the staining susceptibility of enamel after microabrasion using different clinical protocols. Methods. A total of 210 extracted bovine incisors were used in this study. The teeth were randomly divided into five groups of 42 teeth each (n=42), including group 1: control (no treatment), group 2: microabrasion, group 3: microabrasion + fluoride polishing, group 4: macroabrasion (fine-grit diamond bur) + microabrasion, and group 5: macroabrasion (finegrit diamond bur) + microabrasion + fluoride polishing. The groups were then randomly divided into two subgroups for discoloration procedures: coffee and distilled water (n=21). A spectrophotometric evaluation was carried out at baseline and on the 1st, 7th, 14th, and 28th days of the staining period. Statistical analyses were performed using repeated-measures ANOVA and the post hoc Bonferroni test at a significance level of 0.05. Results. The greatest color change was observed in group 2 specimens, which were immersed in coffee solutions. The color change values for groups 3, 4, and 5 specimens, which were immersed in distilled water, were higher than those in group 1 specimens (P<0.05). The fluoride polishing + enamel microabrasion treatment groups (groups 3 and 5) exhibited greater resistance to color changes than the matched non-fluoride-polished groups (groups 2 and 4) (P<0.05). Conclusions. The teeth that underwent enamel microabrasion treatment and were polished with fluoride gel became more resistant to color changes. Our findings confirm that enamel microabrasion treatment is a conservative method for localized discoloration.
{"title":"Effect of microabrasion on the staining susceptibility of enamel: An in vitro study.","authors":"Hasibe Sevilay Bahadir, Merve Haberal, Çiğdem Çelik","doi":"10.34172/joddd.2022.016","DOIUrl":"https://doi.org/10.34172/joddd.2022.016","url":null,"abstract":"<p><p><b>Background.</b> Enamel microabrasion can eliminate enamel irregularities and discoloration. This study evaluated the staining susceptibility of enamel after microabrasion using different clinical protocols. <b>Methods.</b> A total of 210 extracted bovine incisors were used in this study. The teeth were randomly divided into five groups of 42 teeth each (n=42), including group 1: control (no treatment), group 2: microabrasion, group 3: microabrasion + fluoride polishing, group 4: macroabrasion (fine-grit diamond bur) + microabrasion, and group 5: macroabrasion (finegrit diamond bur) + microabrasion + fluoride polishing. The groups were then randomly divided into two subgroups for discoloration procedures: coffee and distilled water (n=21). A spectrophotometric evaluation was carried out at baseline and on the 1st, 7th, 14th, and 28th days of the staining period. Statistical analyses were performed using repeated-measures ANOVA and the post hoc Bonferroni test at a significance level of 0.05. <b>Results.</b> The greatest color change was observed in group 2 specimens, which were immersed in coffee solutions. The color change values for groups 3, 4, and 5 specimens, which were immersed in distilled water, were higher than those in group 1 specimens (<i>P</i><0.05). The fluoride polishing + enamel microabrasion treatment groups (groups 3 and 5) exhibited greater resistance to color changes than the matched non-fluoride-polished groups (groups 2 and 4) (<i>P</i><0.05). <b>Conclusions.</b> The teeth that underwent enamel microabrasion treatment and were polished with fluoride gel became more resistant to color changes. Our findings confirm that enamel microabrasion treatment is a conservative method for localized discoloration.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 2","pages":"95-100"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10421996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cl III malocclusion with a significant skeletal component presents a therapeutic challenge during adolescence. This article presents the encouraging results of an individualized two-stage treatment approach adopted for successful nonsurgical correction of severe skeletal Cl III malocclusion in an adolescent girl after the onset of puberty. An orthopedic approach involving simultaneous alternate rapid maxillary expansion and constriction (Alt-RAMEC) protocol and protraction facemask (PFM) therapy was adopted in phase 1 to correct the sagittal skeletal discrepancy. In phase 2, fixed orthodontic therapy aided by the interim use of a modified occlusal settling appliance was undertaken to obtain well-interdigitated occlusion. Meticulously planned and well-executed orthopedic and orthodontic approach, combined with good patient compliance and favorable growth pattern, helped establish well-balanced facial harmony with a proper maxillomandibular relationship and satisfactory overjet and overbite. The results remained stable during the 4-year follow-up. Alt-RAMEC-PFM therapy accompanied by fixed mechanotherapy is a viable option to treat severe skeletal Cl III malocclusion in adolescents.
{"title":"Management of skeletal Cl III malocclusion using simultaneous alternate rapid maxillary expansion and constriction (Alt-RAMEC) and facemask protraction in adolescence.","authors":"Harpreet Singh, Pranav Kapoor, Poonam Sharma, Raj Kumar Maurya, Tanmay Mittal","doi":"10.34172/joddd.2022.010","DOIUrl":"https://doi.org/10.34172/joddd.2022.010","url":null,"abstract":"<p><p>Cl III malocclusion with a significant skeletal component presents a therapeutic challenge during adolescence. This article presents the encouraging results of an individualized two-stage treatment approach adopted for successful nonsurgical correction of severe skeletal Cl III malocclusion in an adolescent girl after the onset of puberty. An orthopedic approach involving simultaneous alternate rapid maxillary expansion and constriction (Alt-RAMEC) protocol and protraction facemask (PFM) therapy was adopted in phase 1 to correct the sagittal skeletal discrepancy. In phase 2, fixed orthodontic therapy aided by the interim use of a modified occlusal settling appliance was undertaken to obtain well-interdigitated occlusion. Meticulously planned and well-executed orthopedic and orthodontic approach, combined with good patient compliance and favorable growth pattern, helped establish well-balanced facial harmony with a proper maxillomandibular relationship and satisfactory overjet and overbite. The results remained stable during the 4-year follow-up. Alt-RAMEC-PFM therapy accompanied by fixed mechanotherapy is a viable option to treat severe skeletal Cl III malocclusion in adolescents.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 1","pages":"62-69"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40608136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pegah Khamooshi, Maryam Pourhajibagher, Ahmad Sodagar, Abbas Bahador, Badreddin Ahmadi, Sepideh Arab
Background. Microbial accumulation is still a significant problem with removable acrylic appliances. This study aimed to assess the antimicrobial properties of a self-cured acrylic resin containing curcumin nanoparticles (CNPs). Methods. This in vitro study used 48 acrylic discs containing 0.5%, 1%, and 2% CNPs. The antimicrobial properties of the discs against Streptococcus mutans, Streptococcus sanguinis, Lactobacillus acidophilus, and Candida albicans were evaluated using disc agar diffusion (DAD), eluted component, and biofilm inhibition tests. The growth inhibition zones were measured, and the colonies were counted after 1, 3, and 7 days. Results. DAD test showed that none of the curcumin nanoparticle concentrations caused growth inhibition zones for any microorganisms. All the concentrations were effective against all four microorganisms in the biofilm inhibition test except 0.5% for L. acidophilus. In the eluted component test, solutions containing 2% concentration had maximum growth inhibition of all the groups at all time intervals. An increase in curcumin nanoparticle concentration from 0.5% to 1% was effective only against C. albicans. Conclusion. Generally, CNPs in all concentrations were effective against the biofilms of all four microorganisms assessed in this study. Therefore, incorporating 2% CNPs into acrylic resin seems suitable for clinical use.
{"title":"Antibacterial properties of an acrylic resin containing curcumin nanoparticles: An in vitro study.","authors":"Pegah Khamooshi, Maryam Pourhajibagher, Ahmad Sodagar, Abbas Bahador, Badreddin Ahmadi, Sepideh Arab","doi":"10.34172/joddd.2022.032","DOIUrl":"https://doi.org/10.34172/joddd.2022.032","url":null,"abstract":"<p><p><b>Background.</b> Microbial accumulation is still a significant problem with removable acrylic appliances. This study aimed to assess the antimicrobial properties of a self-cured acrylic resin containing curcumin nanoparticles (CNPs). <b>Methods.</b> This in vitro study used 48 acrylic discs containing 0.5%, 1%, and 2% CNPs. The antimicrobial properties of the discs against <i>Streptococcus mutans, Streptococcus sanguinis, Lactobacillus acidophilus,</i> and <i>Candida albicans</i> were evaluated using disc agar diffusion (DAD), eluted component, and biofilm inhibition tests. The growth inhibition zones were measured, and the colonies were counted after 1, 3, and 7 days. <b>Results.</b> DAD test showed that none of the curcumin nanoparticle concentrations caused growth inhibition zones for any microorganisms. All the concentrations were effective against all four microorganisms in the biofilm inhibition test except 0.5% for <i>L. acidophilus</i>. In the eluted component test, solutions containing 2% concentration had maximum growth inhibition of all the groups at all time intervals. An increase in curcumin nanoparticle concentration from 0.5% to 1% was effective only against <i>C. albicans</i>. <b>Conclusion.</b> Generally, CNPs in all concentrations were effective against the biofilms of all four microorganisms assessed in this study. Therefore, incorporating 2% CNPs into acrylic resin seems suitable for clinical use.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 3","pages":"190-195"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10619043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viviane Maria Gonçalves de Figueiredo, Alecsandro de Moura Silva, Marcos Massi, Argemiro Soares da Silva Sobrinho, José Renato Cavalcanti de Queiroz, João Paulo Barros Machado, Renata Falchete do Prado, Lafayette Nogueira Junior
Background. New surface treatments have been proposed to expand the clinical indications of zirconia prostheses. This study aimed to evaluate the effect of silica and fluorine nanofilms on zirconia ceramic on the resin cement bond strength. Methods. Zirconia blocks and discs underwent different surface treatments: untreated zirconia (CON), sandblasted, silica-coated alumina particles (30 µm) (SC), silica nanofilm (SN), and fluorine nanofilm (FN). Nanofilm deposition was performed through plasma enhanced chemical vapor deposition (PECVD). Zirconia surfaces were characterized on disks by morphology (atomic force microscopy, AFM), chemical analysis (x-ray photoelectron spectroscopy, XPS), and contact angle analysis. A silane coupling agent was applied on each treated surface, and a cylinder of resin cement was built up. Half of the specimens in each group were submitted to 6000 thermal cycles (TC). Bond strength was analyzed using the shear test, and the fractographic analysis was performed with stereomicroscopy and SEM/EDS. Statistical analysis was performed through one-way ANOVA and Tukey test in the non-aged and aged specimens. Results. Nanofilms modified the zirconia surface, which became more hydrophilic and chemically reactive. Chemical bonding between Si-O was found in SN, and FN promoted a fluorination process on the ceramic surface, converting zirconia into zirconium oxyfluoride. Specimens of the SN (TC) group failed on pre-testing. FN (TC) bond strength (3.8 MPa) was lower than SC (TC) and CON (TC) after shearing. Adhesive failure predominated in the experimental groups. Silica nanofilm failure occurred after aging. Conclusion. Silica and fluorine nanofilms deposited by PECVD did not promote effective bonding between zirconia and resin cement.
{"title":"Effect of the nanofilm-coated zirconia ceramic on resin cement bond strength.","authors":"Viviane Maria Gonçalves de Figueiredo, Alecsandro de Moura Silva, Marcos Massi, Argemiro Soares da Silva Sobrinho, José Renato Cavalcanti de Queiroz, João Paulo Barros Machado, Renata Falchete do Prado, Lafayette Nogueira Junior","doi":"10.34172/joddd.2022.029","DOIUrl":"https://doi.org/10.34172/joddd.2022.029","url":null,"abstract":"<p><p><b>Background.</b> New surface treatments have been proposed to expand the clinical indications of zirconia prostheses. This study aimed to evaluate the effect of silica and fluorine nanofilms on zirconia ceramic on the resin cement bond strength. <b>Methods.</b> Zirconia blocks and discs underwent different surface treatments: untreated zirconia (CON), sandblasted, silica-coated alumina particles (30 µm) (SC), silica nanofilm (SN), and fluorine nanofilm (FN). Nanofilm deposition was performed through plasma enhanced chemical vapor deposition (PECVD). Zirconia surfaces were characterized on disks by morphology (atomic force microscopy, AFM), chemical analysis (x-ray photoelectron spectroscopy, XPS), and contact angle analysis. A silane coupling agent was applied on each treated surface, and a cylinder of resin cement was built up. Half of the specimens in each group were submitted to 6000 thermal cycles (TC). Bond strength was analyzed using the shear test, and the fractographic analysis was performed with stereomicroscopy and SEM/EDS. Statistical analysis was performed through one-way ANOVA and Tukey test in the non-aged and aged specimens. <b>Results.</b> Nanofilms modified the zirconia surface, which became more hydrophilic and chemically reactive. Chemical bonding between Si-O was found in SN, and FN promoted a fluorination process on the ceramic surface, converting zirconia into zirconium oxyfluoride. Specimens of the SN (TC) group failed on pre-testing. FN (TC) bond strength (3.8 MPa) was lower than SC (TC) and CON (TC) after shearing. Adhesive failure predominated in the experimental groups. Silica nanofilm failure occurred after aging. <b>Conclusion.</b> Silica and fluorine nanofilms deposited by PECVD did not promote effective bonding between zirconia and resin cement.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 3","pages":"170-178"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10619045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. Considering the increased use of preheating and novel resin-based materials to restore teeth, the present study investigated the impact of preheating on the flexural strength of a giomer and compared it with a nanohybrid composite resin. Methods. Two restorative materials (Beautifil II giomer and Alpha III nanohybrid composite resin) were used. Thirty rod-shaped samples (adding up to 60 samples) were prepared from the materials above and divided into two subgroups: with and without preheating (n=15). Before sample preparation, the giomer and nanohybrid composite resin tubes were preheated at 68ºC for 15 minutes in the preheating subgroups. In the subgroups without preheating, the tubes were kept in a room at 25ºC. Then the flexural strength was compared between the two groups with two-way ANOVA at a significance level of P<0.05. Results. The results showed significantly higher flexural strength in the preheated subgroups than in the non-preheated subgroups (P<0.001). In addition, the mean flexural strength values were significantly higher in the giomer groups than in the nanohybrid composite resin groups (P<0.001). Conclusion. Preheating increased the studied materials' flexural strengths significantly. The flexural strength of the giomer restorative material was higher than that of the nanohybrid composite resin, irrespective of preheating.
{"title":"Comparison of the effect of preheating on the flexural strength of giomer and nanohybrid composite resin.","authors":"Soodabeh Kimyai, Zahra Mashayekhi, Narmin Mohammadi, Mahmoud Bahari, Mahdi Abed Kahnamouei, Mohammad Esmaeel Ebrahimi Chaharom","doi":"10.34172/joddd.2022.027","DOIUrl":"https://doi.org/10.34172/joddd.2022.027","url":null,"abstract":"<p><p><b>Background.</b> Considering the increased use of preheating and novel resin-based materials to restore teeth, the present study investigated the impact of preheating on the flexural strength of a giomer and compared it with a nanohybrid composite resin. <b>Methods.</b> Two restorative materials (Beautifil II giomer and Alpha III nanohybrid composite resin) were used. Thirty rod-shaped samples (adding up to 60 samples) were prepared from the materials above and divided into two subgroups: with and without preheating (n=15). Before sample preparation, the giomer and nanohybrid composite resin tubes were preheated at 68ºC for 15 minutes in the preheating subgroups. In the subgroups without preheating, the tubes were kept in a room at 25ºC. Then the flexural strength was compared between the two groups with two-way ANOVA at a significance level of <i>P</i><0.05. <b>Results.</b> The results showed significantly higher flexural strength in the preheated subgroups than in the non-preheated subgroups (<i>P</i><0.001). In addition, the mean flexural strength values were significantly higher in the giomer groups than in the nanohybrid composite resin groups (<i>P</i><0.001). <b>Conclusion.</b> Preheating increased the studied materials' flexural strengths significantly. The flexural strength of the giomer restorative material was higher than that of the nanohybrid composite resin, irrespective of preheating.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 3","pages":"159-163"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10632835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. Although there are various intraoral and extraoral appliances for anchorage management in orthodontics, most fail to preserve the anchorage efficiently. Thus, there is a need for an appliance that can preserve anchorage in the sagittal, vertical, and transverse directions with good patience compliance and cost-effectiveness. This study compared the efficacy of butterfly arch and transpalatal arch (TPA) as an anchorage reinforcing unit during orthodontic space closure using a linear finite element model. Methods. A 3D model of the maxilla and associated structures was developed from CT images of an individual's skull at a slice thickness of 1 mm. The magnitude of movements of anchor teeth in vertical, horizontal, and transverse directions was calculated in first premolar extraction cases during anterior retraction using a linear finite element model analysis and compared in two situations-butterfly arch and TPA attached to maxillary first molar for anchorage. Results. The anterior teeth had similar movements in the case of TPA and butterfly arch. There was more mesial and lingual movement in the first molars with TPA than in the butterfly arch, which had buccal but no mesial movement. The anterior teeth showed extrusion and the second premolars showed intrusion with TPA. Also, the von Mises stress and maximum principal stress were maximum with TPA at the cervical region of anterior and posterior teeth compared to the butterfly arch, where both stresses were uniformly distributed all over the teeth. Conclusion. A butterfly arch with its unique design, configuration, and biomechanical properties can be used as a device that can maintain the posterior anchorage efficiently.
{"title":"Comparison of the effectiveness of butterfly arch versus transpalatal arch in anchorage reinforcement: A linear 3D finite element study.","authors":"Nouf Bano, Sunil Kumar M, Prashantha Govinakovi Shivamurthy, Sharanya Sabrish, Silju Mathew","doi":"10.34172/joddd.2022.017","DOIUrl":"https://doi.org/10.34172/joddd.2022.017","url":null,"abstract":"<p><p><b>Background.</b> Although there are various intraoral and extraoral appliances for anchorage management in orthodontics, most fail to preserve the anchorage efficiently. Thus, there is a need for an appliance that can preserve anchorage in the sagittal, vertical, and transverse directions with good patience compliance and cost-effectiveness. This study compared the efficacy of butterfly arch and transpalatal arch (TPA) as an anchorage reinforcing unit during orthodontic space closure using a linear finite element model. <b>Methods.</b> A 3D model of the maxilla and associated structures was developed from CT images of an individual's skull at a slice thickness of 1 mm. The magnitude of movements of anchor teeth in vertical, horizontal, and transverse directions was calculated in first premolar extraction cases during anterior retraction using a linear finite element model analysis and compared in two situations-butterfly arch and TPA attached to maxillary first molar for anchorage. <b>Results.</b> The anterior teeth had similar movements in the case of TPA and butterfly arch. There was more mesial and lingual movement in the first molars with TPA than in the butterfly arch, which had buccal but no mesial movement. The anterior teeth showed extrusion and the second premolars showed intrusion with TPA. Also, the von Mises stress and maximum principal stress were maximum with TPA at the cervical region of anterior and posterior teeth compared to the butterfly arch, where both stresses were uniformly distributed all over the teeth. <b>Conclusion.</b> A butterfly arch with its unique design, configuration, and biomechanical properties can be used as a device that can maintain the posterior anchorage efficiently.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 2","pages":"101-106"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10414562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. Periradicular surgery is the last treatment option for teeth with persistent periradicular endodontic lesions. This study aimed to assess the adhesion of fibroblasts to root dentin conditioned with ethylenediaminetetraacetic acid (EDTA), MTAD, and QMix. Methods. Twelve dentin discs were fabricated of 6 human single-rooted teeth. Fibroblasts were isolated from the periodontal ligament (PDL) of a premolar tooth. The teeth were healthy and freshly extracted from the socket. The samples were divided into four groups for surface conditioning with (I) EDTA, (II) MTAD, (III) QMix, and the control group. Fibroblasts were cultured on conditioned dentin discs at 37°C, 95% air, and 5% CO2 for 4 hours and then rinsed with PBS three times to eliminate unattached cells from the surface. The mean counts of attached cells were calculated using a Neubauer chamber. Also, the attachment of fibroblasts was evaluated using scanning electron microscopy (SEM). Results. The mean counts of fibroblasts attached to root dentin in EDTA, QMix, MTAD, and control groups were 303±46, 243±41, 213±33, and 347±38, respectively. No significant difference was noted in the number of fibroblasts attached between MTAD, EDTA, and QMix and the control group (P>0.05). Under SEM, the fibroblasts were flat and spindle-shaped, with cytoplasmic processes covering the untreated dentin surface. In the experimental groups, the cells were rounder with fewer processes. All the three groups showed weaker adhesion to dentin compared to the control (untreated dentin) group. Conclusion. Under the limitations of this study, it was concluded that treating the dentin surface with EDTA, MTAD, or QMIX might not be an effective way to improve the adhesion of human PDL fibroblasts.
{"title":"Attachment of human periodontal ligament fibroblasts to root dentin conditioned with different endodontic irrigants: An experimental study.","authors":"Elham Khoshbin, Rezvan Najafi, Maryam Farhadian, Maryam Khalili","doi":"10.34172/joddd.2022.002","DOIUrl":"https://doi.org/10.34172/joddd.2022.002","url":null,"abstract":"Background. Periradicular surgery is the last treatment option for teeth with persistent periradicular endodontic lesions. This study aimed to assess the adhesion of fibroblasts to root dentin conditioned with ethylenediaminetetraacetic acid (EDTA), MTAD, and QMix. Methods. Twelve dentin discs were fabricated of 6 human single-rooted teeth. Fibroblasts were isolated from the periodontal ligament (PDL) of a premolar tooth. The teeth were healthy and freshly extracted from the socket. The samples were divided into four groups for surface conditioning with (I) EDTA, (II) MTAD, (III) QMix, and the control group. Fibroblasts were cultured on conditioned dentin discs at 37°C, 95% air, and 5% CO2 for 4 hours and then rinsed with PBS three times to eliminate unattached cells from the surface. The mean counts of attached cells were calculated using a Neubauer chamber. Also, the attachment of fibroblasts was evaluated using scanning electron microscopy (SEM). Results. The mean counts of fibroblasts attached to root dentin in EDTA, QMix, MTAD, and control groups were 303±46, 243±41, 213±33, and 347±38, respectively. No significant difference was noted in the number of fibroblasts attached between MTAD, EDTA, and QMix and the control group (P>0.05). Under SEM, the fibroblasts were flat and spindle-shaped, with cytoplasmic processes covering the untreated dentin surface. In the experimental groups, the cells were rounder with fewer processes. All the three groups showed weaker adhesion to dentin compared to the control (untreated dentin) group. Conclusion. Under the limitations of this study, it was concluded that treating the dentin surface with EDTA, MTAD, or QMIX might not be an effective way to improve the adhesion of human PDL fibroblasts.","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 1","pages":"11-17"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40608141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Aesthetic expectations have increased the use of aesthetic materials in dentistry. Lithium disilicates are frequently used materials for these expectations. Bleaching is another method used to provide aesthetics. Bleaching processes on restorative materials are not fully known. This study investigated the effect of at-home and in-office bleaching methods on the color change, surface roughness, and topography of lithium disilicate glass-ceramic materials produced with two different techniques and subjected to different polishing procedures.
Methods: A total of 144 disc-shaped pressed and computer-aided design (CAD) lithium disilicate glass-ceramic specimens were randomly divided into four groups. Glazing and three different chair-side polishing procedures were performed. The specimens in each group were randomly divided into two groups and subjected to at-home and in-office bleaching processes (n=9). The home bleaching process was repeated with 16% carbamide peroxide agent for six hours for seven days, while the in-office bleaching process was applied with 40% hydrogen peroxide agent for two sessions of 20 minutes. After the bleaching processes, the final color and surface roughness experiments of the specimens were carried out, and the results were recorded. ANOVA and Tukey multiple comparison tests were used FOR the statistical analysis of the data (α=0.05).
Results: The material*polish*bleaching, polish*bleaching, material*bleaching, and material*polishing interactions were not statistically significant regarding color and roughness changes of both specimens (P>0.05).
Conclusion: Both bleaching processes can be safely applied to lithium disilicate glass-ceramic materials.
{"title":"Effectiveness and safety of bleaching agents on lithium disilicate glass ceramics.","authors":"Alper Ozdogan, Nihan Kaya","doi":"10.34172/joddd.2022.040","DOIUrl":"https://doi.org/10.34172/joddd.2022.040","url":null,"abstract":"<p><strong>Background: </strong>Aesthetic expectations have increased the use of aesthetic materials in dentistry. Lithium disilicates are frequently used materials for these expectations. Bleaching is another method used to provide aesthetics. Bleaching processes on restorative materials are not fully known. This study investigated the effect of at-home and in-office bleaching methods on the color change, surface roughness, and topography of lithium disilicate glass-ceramic materials produced with two different techniques and subjected to different polishing procedures.</p><p><strong>Methods: </strong>A total of 144 disc-shaped pressed and computer-aided design (CAD) lithium disilicate glass-ceramic specimens were randomly divided into four groups. Glazing and three different chair-side polishing procedures were performed. The specimens in each group were randomly divided into two groups and subjected to at-home and in-office bleaching processes (n=9). The home bleaching process was repeated with 16% carbamide peroxide agent for six hours for seven days, while the in-office bleaching process was applied with 40% hydrogen peroxide agent for two sessions of 20 minutes. After the bleaching processes, the final color and surface roughness experiments of the specimens were carried out, and the results were recorded. ANOVA and Tukey multiple comparison tests were used FOR the statistical analysis of the data (α=0.05).</p><p><strong>Results: </strong>The material*polish*bleaching, polish*bleaching, material*bleaching, and material*polishing interactions were not statistically significant regarding color and roughness changes of both specimens (<i>P</i>>0.05).</p><p><strong>Conclusion: </strong>Both bleaching processes can be safely applied to lithium disilicate glass-ceramic materials.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 4","pages":"251-257"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10102922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The purpose of this study was to assess the push-out bond strength of calcium-silicate and silicone based root canal sealers in bulk and with main cone.
Methods: Roots (n=48) randomly divided into 4 groups (n=12) according to the obturation protocol; (1) iRoot SP in bulk; (2) iRoot SP with gutta-percha; (3) GuttaFlow Bioseal in bulk; (4) GuttaFlow Bioseal with gutta-percha. Six horizontal sections were obtained from each root (n=72). Effect of sealers on bond strength was statistically significant (P<0.05).
Results: Highest mean value was obtained in iRoot-Bulk group and lowest in GuttaFlow Bioseal-GP group. Both iRoot SP groups had significantly higher bond strength values than both GuttaFlow Bioseal groups (P<0.05). There was no significant difference between iRoot-GP and iRoot-Bulk groups (P=0.603) also GuttaFlow Bioseal-GP and GuttaFlow Bioseal-Bulk groups (P=0.684).
Conclusion: Based on findings, using calcium silicate-based sealer in bulk can be also suitable in clinical practice.
{"title":"Effect of using calcium-silicate and silicone based root canal sealers in bulk or with main core material on bond strength.","authors":"Gizem Kadı, Esin Özlek, Yousef Saed","doi":"10.34172/joddd.2022.036","DOIUrl":"https://doi.org/10.34172/joddd.2022.036","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this study was to assess the push-out bond strength of calcium-silicate and silicone based root canal sealers in bulk and with main cone.</p><p><strong>Methods: </strong>Roots (n=48) randomly divided into 4 groups (n=12) according to the obturation protocol; (1) iRoot SP in bulk; (2) iRoot SP with gutta-percha; (3) GuttaFlow Bioseal in bulk; (4) GuttaFlow Bioseal with gutta-percha. Six horizontal sections were obtained from each root (n=72). Effect of sealers on bond strength was statistically significant (<i>P</i><0.05).</p><p><strong>Results: </strong>Highest mean value was obtained in iRoot-Bulk group and lowest in GuttaFlow Bioseal-GP group. Both iRoot SP groups had significantly higher bond strength values than both GuttaFlow Bioseal groups (<i>P</i><0.05). There was no significant difference between iRoot-GP and iRoot-Bulk groups (<i>P</i>=0.603) also GuttaFlow Bioseal-GP and GuttaFlow Bioseal-Bulk groups (<i>P</i>=0.684).</p><p><strong>Conclusion: </strong>Based on findings, using calcium silicate-based sealer in bulk can be also suitable in clinical practice.</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 4","pages":"229-233"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10102925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Using antibacterial agents to remove the foul odor of the implant cavity and prevent peri-implantitis can affect the detorque values and lead to the loosening of the abutment screw. This study investigated the effects of tetracycline and chlorhexidine gel on detorque values.
Methods: This in vitro study was carried out on three groups of five implants. Group G1 was the control group, and no material was applied to the implant cavity. In group G2, the implant cavity was first filled with artificial saliva and then with chlorhexidine gel. In group G3, the implant cavity was first filled with artificial saliva and then with tetracycline. The abutments were tightened with 25 N/cm2 and then loosened. Finally, the detorque values were calculated.
Results: The highest detorque values were recorded in group G1. Group G3 showed the lowest detorque values. ANOVA showed significant differences in mean detorque values (P<0.05) between the three groups.
Conclusion: According to this study, applying antibacterial agents decreased the detorque values and increased the risk of screw loosening. The reduction of detorque values was more pronounced with the oil-based antibacterial agent (tetracycline).
{"title":"Comparison of reverse torque values of abutment screws with the application of oil-based and water-based antibacterial agents.","authors":"Amin Nourizadeh, Elnaz Shafiee, Azin Khorramdel, Seyed Amin Mousavi, Mehran Rahbar","doi":"10.34172/joddd.2022.038","DOIUrl":"https://doi.org/10.34172/joddd.2022.038","url":null,"abstract":"<p><strong>Background: </strong>Using antibacterial agents to remove the foul odor of the implant cavity and prevent peri-implantitis can affect the detorque values and lead to the loosening of the abutment screw. This study investigated the effects of tetracycline and chlorhexidine gel on detorque values.</p><p><strong>Methods: </strong>This in vitro study was carried out on three groups of five implants. Group G1 was the control group, and no material was applied to the implant cavity. In group G2, the implant cavity was first filled with artificial saliva and then with chlorhexidine gel. In group G3, the implant cavity was first filled with artificial saliva and then with tetracycline. The abutments were tightened with 25 N/cm<sup>2</sup> and then loosened. Finally, the detorque values were calculated.</p><p><strong>Results: </strong>The highest detorque values were recorded in group G1. Group G3 showed the lowest detorque values. ANOVA showed significant differences in mean detorque values (<i>P</i><0.05) between the three groups.</p><p><strong>Conclusion: </strong>According to this study, applying antibacterial agents decreased the detorque values and increased the risk of screw loosening. The reduction of detorque values was more pronounced with the oil-based antibacterial agent (tetracycline).</p>","PeriodicalId":15599,"journal":{"name":"Journal of Dental Research, Dental Clinics, Dental Prospects","volume":"16 4","pages":"238-242"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10104908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}